CN110218678A

CN110218678A - Anti- continuous cropping soil renovation agent and preparation method thereof

Info

Publication number: CN110218678A
Application number: CN201910537777.4A
Authority: CN
Inventors: 张传忠; 王玉梅; 高祥照
Original assignee: Henan Railway Locomotive Agricultural Technology Co ltd
Current assignee: Henan Railway Locomotive Agricultural Technology Co ltd
Priority date: 2019-06-20
Filing date: 2019-06-20
Publication date: 2019-09-10
Anticipated expiration: 2039-06-20
Also published as: CN110218678B

Abstract

The present invention relates to a kind of raising crop resistances, increasing yield and improving quality, anti- continuous cropping soil renovation agent of chesson and preparation method thereof, bacillus subtilis antagonistic strain Z-15 scientific name bacillus subtilis, culture presevation number GDMCC NO:60653, including 4 ~ 6 parts of fatty acid cane sugar ester, 4 ~ 6 parts of fatty alcohol polyoxyethylene ether, 3 ~ 5 parts of calcium dodecyl benzene sulfonate, 3 ~ 5 parts of ethylene glycol, 2 ~ 3 parts of bacillus subtilis Z-15, 2 ~ 3 parts of bacillusmusilaginosiengineering, 5 ~ 8 parts of zinc sulfate, 5 ~ 8 parts of manganese sulfate, 5 ~ 8 parts of Boratex, 56 ~ 60 parts of solid support medium, it is low that continuous cropping soil disease resistance can be improved, low output, soil compaction phenomenon, soil colloid is set to adsorb hydrogen ion separate out, increase Cation Exchange Capacity in Soils, improve soil texture, it builds up fertility, Keep water nutrition, improve soil physical and chemical property, reduce the soil weight, increase soil porosity, chesson promotes crop absorbed nitrogen, phosphorus, potassium and middle microelement, makes crop yield upgrading, bacillus subtilis prevents and treats soil-borne disease, inhibits harmful growth of pathogenic bacteria or propagation, enhances the resistivity of plant pair pathogen.

Description

Anti- continuous cropping soil renovation agent and preparation method thereof

Technical field

To improve, soil disease resistance is poor, crop yield is low, soil hardening caused by continuous cropping soil the present invention relates to a kind of Phenomenon, it is intended to for the disease resistance of raising crop, chesson, the anti-continuous cropping soil renovation agent of increasing yield and improving quality and preparation method thereof.

Background technique

China be individual it is more, few big country, it is relatively common that regionality plants same crop, and planting site for years Block is more, will appear under the conditions of normal cultivation management in this case growing way die down, soil pest and disease damage increase, soil Earth nutrient numerous imbalances, soil physico-chemical property deteriorate, soil enzyme activities changes, (crop passes through crop generation Auto toxicity The approach such as overground part leaching, root exudates and plant stubble discharge some suppression harmful bacterias, when suppression harmful bacteria reaches a certain amount of Afterwards, can or equal plant growth of the same race to same stubble or lower stubble generate inhibiting effect), cause soil degradation area larger, crop disease Insect pest is serious, has adverse effect on to the growth and development of crop, leads to that the disease resistance of crop is poor, soil hardening, thus That limits output further increases the improvement with quality.

For this problem, the crop that can make on the soil of continuous cropping there is presently no one, by improving soil physical chemistry ring Border, the crumb structure for increasing soil permeability, increasing soil, water, fertilizer, air and heat and the advanced microbial technique for adjusting soil It combines, it is complimentary to one another using the reciprocation between different material in addition to single effect, reach the effect of 2+2 > 5, so that The soil environment of continuous cropping plantation is improved year after year, solves the adverse consequences of continuous cropping bring undesirable element and generation.

It is applied in soil improvement currently, everybody starts with beneficial microbe to improve soil texture, improves water conservation, protects Fertile ability promotes largely necessary element and the microelement such as crop absorbed nitrogen, phosphorus, potassium, and reaching makes promoting crop growth increase production purpose. Microbial bacterial agent is applied for a patent by many inventors as a kind of environmental friendly fertilizer at present, is become a kind of and is solved not Carry out one of the important directions of agricultural sustainable development.Such as patent application publication CN103103149 A, disclosing one kind has The bacillus subtilis S001 of broad spectrum antibacterial activity and growth-promoting ability, patent application publication CN107056524 A, discloses A kind of novel microbial nursery nutrient solution and preparation method thereof, above-mentioned patent application, which has, promotes crop growth, promoting root growth, strong Seedling, antibacterial, disease-resistant feature prepare microbial pesticide and microorganism fertilizer using the antibacterial and growth-promoting ability of bacillus subtilis Material.In addition to the above literature, it is disclosed there are also many microbial manures, but its major part collects the research of microbial bacterial agent more In in fixed nitrogen, decompose soil organic substance and slightly solubility mineral, the disease-resistant fields such as with stimulation plant growth, Root Symbiont bacterium.To the greatest extent The patent application for managing these many microbial bacterial agents largely occurs, but general lack of high efficient strain, microorganism resource lacks, very much The strain of producer is all utilizing the same bacterial strain, and bacterial strain is single, in face of current complicated soil degradation situation, relies solely on one kind Strain is difficult to solve soil issues, for soil improvement, the effective microbial bacteria bolus of crop yield is promoted to be rarely reported, i.e., Two or more strains has been used to overcome the above problem, when using multi-cultur es improvement soil, because lacking synergy The protection of composition and other active materials, and without the supplement of other microelements, causes that effect is poor, coordinating protection ability Low, activity is difficult to the defects of reaching peak, stability and poor compatibility.

Complex microorganism can produce synergistic effect in the soil, complimentary to one another, effectively improve soil texture, improve soil Earth fertility keeps soil moisture and nutrition, reduces soil pH value and salt content, promotes a great number of elements such as crop absorbed nitrogen, phosphorus, potassium And middle microelement, achieve the purpose that increase production promoting crop growth, but each microorganism collective effect how is coordinated, it plays It is still this field technical problem urgently to be solved at present to optimum efficiency.

Summary of the invention

Caused by overcoming the deficiencies in the prior art and a kind of improvement continuous cropping soil be provided Soil-borne disease is serious, disease resistance is poor, low output, the serious anti-continuous cropping soil renovation agent of soil hardening and preparation method thereof, with solution Higher cost certainly of the existing technology and it is ineffective the problems such as.

To achieve the goals above, technical solution provided by the invention is achieved in that

A kind of bacillus subtilis antagonistic strain Z-15, the taxology scientific name of bacillus subtilis antagonistic strain Z-15 are withered grass buds Spore bacillus (Bacillus subtilis) is preserved in Guangdong Province's Culture Collection on May 5th, 2019, Address is 5 building, No. 59 building of Xianlie Middle Road, Guangzhou City 100, and culture presevation number is GDMCC NO:60653.

A kind of anti-continuous cropping soil renovation agent made using the bacillus subtilis antagonistic strain Z-15, the anti-continuous cropping soil Earth renovation agent is made including the following raw material in parts by weight:

(1) active agent component: 4 ~ 6 parts of fatty acid cane sugar ester, 4 ~ 6 parts of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3 ~ 5 Part, 3 ~ 5 parts of ethylene glycol；

(2) micropopulation: 2 ~ 3 parts of bacillus subtilis Z-15,2 ~ 3 parts of bacillusmusilaginosiengineering;

(3) microelement component: 5 ~ 8 parts of zinc sulfate, 5 ~ 8 parts of manganese sulfate, 5 ~ 8 parts of Boratex；

(4) solid support medium: 56 ~ 60 parts of the solid support medium containing crude fat.

The anti-continuous cropping soil renovation agent form is solid-state.

The fatty acid cane sugar ester is generated by sucrose and fatty acid through esterification at a temperature of 40~45 DEG C.

The fatty alcohol polyoxyethylene ether is to make catalyst with sodium hydroxide, and long-chain fatty alcohol exists in anhydrous and no oxygen In the case where with ethylene oxide occur ring-opening polymerization made from.

The solid support medium refers to the solid support medium containing crude fat, is the sesame with pure raw sesame cake fermentation maturity Cake fertilizer, crude fat weight percentage >=5%, organic matter weight percentage >=50%, moisture weight percentage composition≤20%, pH 5.5 ~ 8.5, under the conditions of this, special better living environment and nutrition are created to bacillus subtilis antagonism strain Z-15 Supply, is more advantageous to the breeding of the strain.

A kind of preparation method of the anti-continuous cropping soil renovation agent, specific production step are as follows:

Step 1) fatty alcohol polyoxyethylene ether is to make catalyst with sodium hydroxide, and long-chain fatty alcohol is existing for the anhydrous and no oxygen In the case of with ethylene oxide occur ring-opening polymerization be made；

Step 2 fatty acid cane sugar ester is generated by sucrose and fatty acid through esterification at a temperature of 40~45 DEG C；

Solid support medium of the step 3) containing crude fat is that fermentation liquid is added after pure raw sesame cake crushes, 50 are increased in temperature~ After 65 DEG C, stir stirring, aeration work, fermented and manufactured, high temperature is mainly for effectively killing the disease in raw material Bacterium worm's ovum, and large quantity of moisture is evaporated, pay special attention to ferment hot stage when temperature reaches 65 DEG C or so in mid-term, it is timely Turning is unlikely to heap temperature more than 70 DEG C, so that crude fat weight percentage >=5% in the sesame cake fertilizer fermented, organic Matter weight percentage >=50%, moisture weight percentage composition≤20%, pH 5.5 ~ 8.5 under the conditions of this, give bacillus subtilis Bacterium antagonism strain Z-15 creates the supply of special better living environment and nutrition, is more advantageous to the breeding of the strain.

Under the conditions of temperature is 32~36 DEG C, 6.5 ~ 7.0 pH, bacillus subtilis antagonistic strain Z-15's step 4) exists 48 h are cultivated on culture medium；

For step 5) under the conditions of temperature is 30 ~ 36 DEG C, 7.0 ~ 7.5 pH, bacillusmusilaginosiengineering bacterial strain cultivates 33 on culture medium h；

Step 6) forms the fatty alcohol polyoxyethylene ether prepared, fatty acid cane sugar ester, calcium dodecyl benzene sulfonate, ethylene glycol Active agent component, zinc sulfate, manganese sulfate and Boratex composition microelement component be added to according to parts by weight containing subtracting In the twin-shaft mixer for shaking seat, after carrying out concussion mixing, under normal temperature conditions, carries out that 50 min are sufficiently stirred, then shift After carrying out sufficiently reaction in closed instrument, container is opened, it is aeration-cooling to arrive room temperature；

It is good that cultured bacillus subtilis antagonistic strain Z-15 and bacillusmusilaginosiengineering are placed on fermentation maturity by step 7) Containing crude fat solid support medium (sesame cake fertilizer of pure raw sesame cake fermentation maturity, crude fat content >=5%, organic matter >= 50%, moisture≤20%, pH 5.5 ~ 8.5) in blending, bred in the environment of adaptation, for use；

The cultured sample of step 7) is placed in blender by step 8), then is put into step 6) sample is prepared, and is carried out abundant It mixes, then sub-sieve, metering, packaging, storage.

The novel bacillus subtilis antagonistic strain Z-15 is by my company and Agricultural University Of Hebei's life science Institute's cooperative research and development, there is broad spectrum antibiotic activity and extremely strong anti-adversity ability, a variety of antibiotic and mould substance can be secreted, stimulate The unfavorable growing environment of harmful microbe prevents and treats soil-borne disease, inhibits the growth or propagation of harmful pathogen, enhances plant to disease The resistivity of opportunistic pathogen.

The living bacteria count >=2.0 hundred million/g.

The present invention has following good effect: the present invention relates to one kind to improve soil disease resistance caused by continuous cropping soil Difference, phenomena such as crop yield is low, soil hardening, it is intended to for improve the disease resistance of crop, increasing yield and improving quality, chesson anti-continuous cropping Phenomena such as soil-repairing agent and preparation method thereof, disease resistance, the crop yield that can improve continuous cropping soil be low, soil hardening.Soil Colloid can make the hydrogen ion separate out of adsorption by soil, increase Cation Exchange Capacity in Soils, effectively improve soil texture, improve Soil fertility keeps soil moisture and nutrition, improves soil physical and chemical property, reach reduce the soil weight, increase soil porosity, Chesson purpose promotes a great number of elements and the middle microelements such as crop absorbed nitrogen, phosphorus, potassium, and reaching makes crop yield upgrading Purpose, and bacillus subtilis antagonistic strain Z-15 can stimulate the unfavorable growing environment of harmful microbe, prevention and treatment soil pass Disease, the characteristic for inhibiting pathogen to propagate.

The activating agent that the present invention is added passes through the physical chemistry absorbent properties principle of soil, activates having for it using the soil water Ingredient is imitated, is reacted and is played a role in the soil by soil stomata, carry out the ion exchange of soil, make the hydrogen of adsorption by soil Ion separate out increases Cation Exchange Capacity in Soils, makes the more holes of Zinc fractions, improves soil aggregate structure, enhancing The gas permeability and rich water penetrating power of soil, to reach the mesh for reducing the soil weight, increasing soil porosity, chesson 's.

The effective microorganisms body can kill the fraction of pathogens bacterial strain in soil, and secretion, which generates one kind, can eliminate soil The toxin of middle pathogen.The unfavorable growth that bacillus subtilis antagonistic strain Z-15 passes through stimulation harmful microorganism and pathogen Environment, thus the characteristic for preventing and treating soil-borne disease, pathogen being inhibited to propagate.Bacillus subtilis antagonistic strain Z-15 was being grown Cheng Zhongneng generates a variety of with antibacterial, bacteriolysis substance, such as subtilin, organic acid, antibacterial protein, these substances It can inhibit the growth and breeding of pathogen, or even destroy bacterial structure, kill pathogen.Therefore, bacillus subtilis antagonism Bacterial strain Z-15 is fine for disease prevention and cure effects such as continuous cropping disease, root rot, gray molds.Bacillus subtilis antagonistic strain Z-15 It colonizes at positions such as root of the crop table, the tip of a root, ground diameters in reproductive process, the Fungicidal substances such as lipopeptid class and protide can be generated, from And crop pathogens are killed, reach protection effect.Bacillus subtilis antagonistic strain Z-15 can be activated with secretion activity substance Plant defense system enhances the immunity and disease resistance of crop, and harm of the pathogen to plant is mitigated or eliminated, may additionally facilitate more The growth and development of kind vegetable seeds, seedling and root system, enhances the disease resistance of plant, to reduce disease indirectly, such as Increasing the formation of the auxin such as heteroauxin, stimulation crop root is developed, enhance photosynthesis, while hardly possible in soil being absorbed Substance is converted into the substance for being easy to crop absorption, promotes crop that nutriment is absorbed and utilized, and improves utilization rate of fertilizer, promotes Crop root and plant strain growth improve the disease resistance of crop.Bacillus subtilis antagonistic strain Z-15 not only can directly inhibit Phytopathogen, and the disease resistance of plant, such as rice sheath blight disease can be enhanced by the disease-resistant potential of inducing plant itself Biocontrol microorganisms bacillus subtilis can induce disease-resistant relevant enzyme (POD, the PPO and SOD) increased activity of rice leaf sheath cell, reach Disease resisting effect.Bacillus subtilis antagonistic strain Z-15 forms probiotics environment in the soil, promotes crumb structure to be formed, mentions High soil fertilizer water holding capacity increases loosing soil degree, promotes root growth.It is in particular in, accelerates nutriment mineralization, rest Dividing from invalid state and slow state becomes available state and quick-acting states.Meanwhile accelerating nutriment humification, and secretion of phytase, soil of degrading In most phytate；Auxin is generated, plant growth is stimulated, improves the survival rate and emergence rate of seed.Bacillus subtilis A variety of effective antibacterial materials have been isolated and purified in the metabolite of bacterium different strains.Bacillus subtilis antagonistic strain Z-15 Bacteriolysis be mainly manifested in be by being adsorbed on the mycelia of pathogen, and with mycelia grow and grow, then generate Plasm is caused to reveal for lysozyme so that mycelium is broken；It either generates antibacterial material and passes through the thin of dissolution pathogen spore Cell wall or cell membrane, phenomena such as causing cell wall perforation, deformity, is to inhibiting spore germination.Plant is induced to generate resistance and promotion Plant growth refers to that bacillus subtilis is not only able to inhibit phytopathogen, but also being capable of inducing plant itself disease resistance mechanisms To enhance the effect of the disease-resistant performance of plant, wherein the most strong, function with the resistance of bacillus subtilis antagonistic strain Z-15 At most, adaptability is most wide, effect is most stable.Bacillus subtilis antagonistic strain Z-15 can generate similar cell mitogen, plant The substance of object growth hormone promotes the growth of plant to make the infringement of plant resistant pathogen.Bacillus subtilis antagonistic strain Z- 15 when acting on soil, can be in crop rhizosphere or Colonization inside plants, and plays specific fertilizer effect.Bacillus subtilis is to soil In phenanthrene be known as soil-rice system system with the absorption of BaP and biological degradation function, the coupled water environment of soil, In there is a large amount of soil autochthonous microorganisms, and on surface there are biomembrane, because of biofilm formation separation layer, organic dirt Dye object is before the solid substrate for touching support biomembrane, it is necessary to arrive first at and pass through this separation layer, thus by force The absorption behavior for changing mineral grain or substrate strongly has important influence to suction-operated, in recent years studies have shown that due to by Pollution effect causes to contain polycyclic aromatic hydrocarbon (PAHs) in soil, and PAHs is mainly crude oil pollution and industry or the people in deposit Caused by coal imperfect combustion, bacillus subtilis studies the luxuriant and rich with fragrance absorption with BaP and biodegrade, research shows that with withered Careless bacillus is microbe inoculation, can all carry out absorption to luxuriant and rich with fragrance and BaP or biodegrade, 48 h liquid phase P AHs concentration reach To when balancing, microorganism eliminates 98% to phenanthrene, eliminates 85% to BaP；The 48 h adsorption isotherm of sample of inoculation is in line Shape can better conform to linear equation；In microbe inoculation, surface sediments and soils are equal to luxuriant and rich with fragrance and BaP adsorpting characteristic It varies widely, about 35 times is increased to luxuriant and rich with fragrance adsorbance, and 2/3 or so is reduced to the adsorbance of BaP；It is not inoculated with The soils and sediments of microorganism are 20% to luxuriant and rich with fragrance desorption efficiency, and the sample sets of inoculation are 2.9%, and to the desorption result of BaP with For phenanthrene on the contrary, nonvaccinated control group is 4%, the sample sets of inoculation are 3%.Intensity of soil respiration as biological activity of soil index it One, the conversion and deliverability of soil nutrient object, respiratory rate variation and change direction can be reacted to a certain extent The ecosystem has been reacted to the sensitivity and response modes of stress, is an important indicator of environmental safety assessment, is worked as soil When being polluted by external contaminant, microorganism may need more energy to survive, and make the generation of edaphon It thanks to activity and different degrees of response occurs.Research shows that the bacillus subtilis of each mass fraction processing shows as exhaling soil The stimulating effect of suction effect, and bacillus subtilis mass fraction is bigger in soil, to the stimulation of intensity of soil respiration Bigger, i.e. stimulus intensity and application mass fraction is positively correlated.Using soil enzyme as monitoring index, the ecology poison of pesticide is evaluated Reason effect has become one of research hotspot of environmental science, and urase belongs in soil a kind of water for studying more deep Enzyme is solved, is uniquely to be converted in the soil to urea and urea desorption column has the enzyme of significant impact.After urea is manured into soil, in urea Under the catalytic action of enzyme, carbon dioxide and ammonia are resolved into rapidly, so the reduction of soil urease liveness, can not only make hydrolysis of urea Slow down, enables its hydrolysate more be effectively reduced the volatilization loss of hydrolysis of urea product ammonia by adsorption by soil, it is also possible to phase Hydrolysate NH soil nitrification potential should be reduced.Research shows that all processing are with the processed soil of bacillus subtilis to soil Urase shows stimulating effect, and wherein highest mass fractions processing (3200mg/kg dry ground) rose in the 28th day urease activity To highest, stimulation rate reaches 101.07%.The mechanism that bacillus subtilis stimulates urase, it may be possible to due to microbial pesticide The growth of artificial microorganism is added to provide carbon source and nutrition, thus increase the micro organism quantity for generating this kind of enzyme, increased activity, The thus activity of urase also corresponding enhancing in soil, bacillus subtilis can be to alkaline land improving.

The bacillusmusilaginosiengineering has phosphorus decomposing, potassium decomposing effect, activates phosphorus potassium activity in soil, promotes crop root Growth improves soil and fertilizer utilization rate, increases the microbial bacteria population number in soil.Bacillusmusilaginosiengineering can decompose in mineral Potassium, silicon, can also decomposite the phosphorus in apatite, have the function of Potassium release and fixed nitrogen, while can generate during growth and breeding The substance that organic acid, amino acid, polysaccharide, hormone etc. are conducive to plant absorption and utilize.After breeding in the soil, plant can be secreted Growth stimulant and a variety of enzymes, to enhance crop to the resistance of some pest and disease damages；Also inhibit the growth of other pathogens.

The microelement is capable of providing the supply of part nutrient, nutrient caused by supplement continuous cropping plantation It is lost, alleviates the autotoxicity that continuous cropping crop generates.

The solid support medium containing crude fat is the sesame cake fertilizer that fermentation maturity is carried out using pure raw sesame cake, interior Containing organic matter abundant (>=50%), crude fat (>=5%), protein, amino acid, microelement, sugar and part nitrogen, phosphorus, potassium Abundant nutrition carrier.

The reciprocation that above-mentioned substance is grouped together can effectively improve soil texture, increase soil fertility, keep Soil moisture and nutrition improve soil physical and chemical property, promote a great number of elements and the middle microelements such as crop absorbed nitrogen, phosphorus, potassium, Reaching makes promoting crop growth increase production purpose, and bacillus subtilis antagonistic strain Z-15 can stimulate the unfavorable life of harmful microbe Long environment, characteristic prevention and treatment soil-borne disease, pathogen is inhibited to propagate.

The present invention tests in Different Crop, demonstrating obtains following result:

Under fertilizer application condition of the same race, the Xiayi County town Hui Ting watermelon in 2019 applies anti-continuous cropping soil renovation agent actual observation result card Bright, the diseased plant rate that watermelon applies anti-continuous cropping soil renovation agent reduces by 4.4 percentage points than control, and cell watermelon average weight compares According to increasing by 30.8 kg, increment rate is 33.89 %, and sugar content increases by 0.83 percentage point, and under the conditions of identical topsoil, soil is tight Solidity reduces 1.65kg/cm², the amplitude that reduces is 9.52%.2019 Nian Wuli poplar watermelon diseased plant rates the survey showed that continuous cropping greenhouse After watermelon applies anti-continuous cropping soil renovation agent, diseased plant rate reduces by 4.4 percentage points, and fruit deformation rate reduces by 0.6 percentage point.

Under fertilizer application condition of the same race, Yantai apple application the result shows that: anti-continuous cropping soil renovation agent can reduce soil Bulk density has the function of chesson；It can promote the development of root system of the apple, root system is healthy and strong, capillary root is more；Increase production apple 965kg/ mus, rate of growth 31.7%, volume increase reaches extremely significant level；In the side such as apple color, fruit size, apple commodity rate Face is all substantially better than control；Diseased fruit rate reduces 5.3%, and I and II fruit rate increases 5%, and the output value increases 6598 yuan/mu, only 6588.6 yuan/mu of volume increase value, input-output ratio 1:59.3.

Under fertilizer application condition of the same race, grape applies anti-continuous cropping soil renovation agent and alleviates the few jelly by extreme cold weather of grape Evil applies the Table Grape yield of anti-continuous cropping soil renovation agent up to 1798.01 kg, relatively compares (per mu yield Table Grape 1314.66 Kg) increase production 480.35 kg, volume increase is up to 36.76%, and average yield per mu value increases by 5764.2 yuan, remarkable in economical benefits, berry sugar content Average out to 16.86%(n ﹦ 5), compare berry sugar content average out to 15.76%(n ﹦ 5), berry sugar content application relatively control increases by 1.1 Percentage point.It is opposite to improve 6.98%.The soil profile discovery in vineyard is observed simultaneously, and loosing soil, grape root system is more, root system Healthy and strong flourishing, the soil weight reduces by 0.5 ~ 1.2 g/cm³, reduce by 56 ~ 135 N/cm of soil hardness²。

Under fertilizer application condition of the same race, tobacco, which applies anti-continuous cropping soil renovation agent, reduces flower caused by the plantation of tobacco leaf many years continuous cropping Leaf disease and balck shank, tobacco mosaic disease rate decline 2~58.7 percentage points, relative drop amplitude 15.4%~96.5%；Tobacco is black Shin disease diseased plant rate declines 6~30 percentage points, and relative drop amplitude 16.3%~65.2%, the Yunnan village Hua Luping observation point is even Up to 100%, effect highly significant, reduce plant leaf absorb heavy metal (lead, chromium, cadmium, mercury, arsenic content reduce respectively 1.39 mg/kg, 3.01 mg/kg, 0.102 mg/kg, 0.02mg/kg, 0.012 mg/kg), reduce containing for pesticide in plant Amount improves quality of tobacco, increases by 640.51 ~ 1374.05 yuan/mu of tobacco grower's economic benefit.

Specific embodiment

A kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent are made including the following raw material in parts by weight:

The anti-continuous cropping soil renovation agent form is solid-state.

The solid support medium refers to the solid support medium containing crude fat, is the sesame with pure raw sesame cake fermentation maturity Cake fertilizer, crude fat weight percentage >=5%, organic matter >=50%, moisture≤20%, pH 5.5 ~ 8.5.Under the conditions of this, to withered Careless bacillus antagonism strain Z-15 creates the supply of special better living environment and nutrition, is more advantageous to the numerous of the strain It grows.

A kind of preparation method of the anti-continuous cropping soil renovation agent, the specific steps are as follows:

Solid support medium of the step 3) containing crude fat is that fermentation liquid is added after pure raw sesame cake crushes, 50 are increased in temperature~ After 65 DEG C, stir stirring, aeration work, fermented and manufactured, high temperature is mainly for effectively killing the disease in sample Bacterium worm's ovum, and large quantity of moisture is evaporated, pay special attention to ferment hot stage when temperature reaches 65 DEG C or so in mid-term, it is timely Turning is unlikely to heap temperature more than 70 DEG C so that crude fat content >=5% in the sesame cake fertilizer fermented, organic matter >=50%, Moisture≤20%, pH 5.5 ~ 8.5.Under the conditions of this, special better life is created to bacillus subtilis antagonism strain Z-15 The supply of dis environment and nutrition is more advantageous to the breeding of the strain.

Under the conditions of temperature is 32~36 DEG C, 6.5 ~ 7.0 pH, bacillus subtilis antagonistic strain Z-15's step 4) exists 48h is cultivated on culture medium；

The living bacteria count >=2.0 hundred million/g.

Embodiment 1: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(1) active agent component: 6 kilograms of fatty acid cane sugar ester, 4 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram, 3 kilograms of ethylene glycol；

(2) micropopulation: 2 kilograms of bacillus subtilis Z-15,2 kilograms of bacillusmusilaginosiengineering;

(3) microelement component: 5 kilograms of zinc sulfate, 5 kilograms of manganese sulfate, 5 kilograms of Boratex；

(4) solid support medium: 56 kilograms of the solid support medium containing crude fat.

The anti-continuous cropping soil renovation agent form is solid-state.

Solid support medium of the step 3) containing crude fat is that fermentation liquid is added after pure raw sesame cake crushes, 50 are increased in temperature~ After 65 DEG C, stir stirring, aeration work, fermented and manufactured, high temperature is mainly for effectively killing the disease in raw material Bacterium worm's ovum, and large quantity of moisture is evaporated, pay special attention to ferment hot stage when temperature reaches 65 DEG C or so in mid-term, it is timely Turning is unlikely to heap temperature more than 70 DEG C so that crude fat content >=5% in the sesame cake fertilizer fermented, organic matter >=50%, Moisture≤20%, pH 5.5 ~ 8.5.Under the conditions of this, special better life is created to bacillus subtilis antagonism strain Z-15 The supply of dis environment and nutrition is more advantageous to the breeding of the strain.

The novel bacillus subtilis antagonistic strain Z-15 is joined by my company and Agricultural University Of Hebei's Life Science College Research and development are closed, there is broad spectrum antibiotic activity and extremely strong anti-adversity ability, a variety of antibiotic and mould substance can be secreted, stimulation is harmful The unfavorable growing environment of microorganism prevents and treats soil-borne disease, inhibits the growth or propagation of harmful pathogen, enhances plant to pathogen Resistivity.

The living bacteria count >=2.0 hundred million/g.

Embodiment 2: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram, 3 kilograms of ethylene glycol；

The anti-continuous cropping soil renovation agent form is solid-state.

Embodiment 3: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 4 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 5,000 Gram, 3 kilograms of ethylene glycol；

Embodiment 4: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 4 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram, 5 kilograms of ethylene glycol；

Embodiment 5: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 4 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram, 3 kilograms of ethylene glycol；

(2) micropopulation: 3 kilograms of bacillus subtilis Z-15,2 kilograms of bacillusmusilaginosiengineering;

Embodiment 6: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 4 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram part, 3 kilograms of ethylene glycol；

(2) micropopulation: 2 kilograms of bacillus subtilis Z-15,3 kilograms of bacillusmusilaginosiengineering;

Embodiment 7: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(3) microelement component: 8 kilograms of zinc sulfate, 5 kilograms of manganese sulfate, 5 kilograms of Boratex；

Embodiment 8: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

Embodiment 9: a kind of anti-continuous cropping soil renovation agent, which includes the following raw material It is made:

(3) microelement component: 5 kilograms of zinc sulfate, 8 kilograms of manganese sulfate, 5 kilograms of Boratex；

Embodiment 10: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(3) microelement component: 5 kilograms of zinc sulfate, 5 kilograms of manganese sulfate, 8 kilograms of Boratex；

Embodiment 11: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(4) solid support medium: 60 kilograms of the solid support medium containing crude fat.

Embodiment 12: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 5 kilograms of fatty acid cane sugar ester, 5 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 4,000 Gram, 4 kilograms of ethylene glycol；

(3) microelement component: 7 kilograms of zinc sulfate, 7 kilograms of manganese sulfate, 7 kilograms of Boratex；

(4) solid support medium: 58 kilograms of the solid support medium containing crude fat.

Embodiment 13: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 5,000 Gram, 5 kilograms of ethylene glycol；

(2) micropopulation: 3 kilograms of bacillus subtilis Z-15,3 kilograms of bacillusmusilaginosiengineering;

(3) microelement component: 8 kilograms of zinc sulfate, 8 kilograms of manganese sulfate, 8 kilograms of Boratex；

Embodiment 14: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 6 kilograms of fatty acid cane sugar ester, 4 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 5,000 Gram, 5 kilograms of ethylene glycol；

Embodiment 15: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 6 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram, 5 kilograms of ethylene glycol；

Embodiment 16: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 6 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 5,000 Gram, 3 kilograms of ethylene glycol；

Embodiment 17: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 6 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 5,000 Gram, 5 kilograms of ethylene glycol；

Embodiment 18: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

Embodiment 19: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(3) microelement component: 5 kilograms of zinc sulfate, 8 kilograms of manganese sulfate, 8 kilograms of Boratex；

Embodiment 20: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(3) microelement component: 8 kilograms of zinc sulfate, 5 kilograms of manganese sulfate, 8 kilograms of Boratex；

Embodiment 21: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(3) microelement component: 8 kilograms of zinc sulfate, 8 kilograms of manganese sulfate, 5 kilograms of Boratex；

Embodiment 22: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

Embodiment 23: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 3,000 Gram, 5 kilograms of ethylene glycol；

(4) solid support medium: 57 kilograms of the solid support medium containing crude fat.

Embodiment 24: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 5 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 4,000 Gram, 5 kilograms of ethylene glycol；

(3) microelement component: 7 kilograms of zinc sulfate, 6 kilograms of manganese sulfate, 7 kilograms of Boratex；

(4) solid support medium: 59 kilograms of the solid support medium containing crude fat.

Embodiment 25: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 5.5 kilograms of fatty acid cane sugar ester, 6 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 4.5 kilograms, 4.5 kilograms of ethylene glycol；

(2) micropopulation: 2.5 kilograms of bacillus subtilis Z-15,2.5 kilograms of bacillusmusilaginosiengineering;

(3) microelement component: 6 kilograms of zinc sulfate, 7 kilograms of manganese sulfate, 8 kilograms of Boratex；

Embodiment 26: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 6 kilograms of fatty acid cane sugar ester, 5 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 4,000 Gram, 5 kilograms of ethylene glycol；

Embodiment 27: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

Embodiment 28: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(3) microelement component: 6 kilograms of zinc sulfate, 6 kilograms of manganese sulfate, 6 kilograms of Boratex；

Embodiment 29: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

(1) active agent component: 4 kilograms of fatty acid cane sugar ester, 5 kilograms of fatty alcohol polyoxyethylene ether, calcium dodecyl benzene sulfonate 5,000 Gram, 3 kilograms of ethylene glycol；

(3) microelement component: 7 kilograms of zinc sulfate, 8 kilograms of manganese sulfate, 5 kilograms of Boratex；

Embodiment 30: a kind of anti-continuous cropping soil renovation agent, the anti-continuous cropping soil renovation agent grams by weight include following former Material is made:

Test 1: the application effect of the Xiayi County town Hui Ting watermelon application " anti-continuous cropping soil renovation agent " is reported:

As the supply and demand amount of watermelon increasingly increases, plantation watermelon amount is more and more, due to planting west in same soil for a long time When melon or close relative edge crop, it just will appear continuous cropping phenomenon.And watermelon is a kind of plant for most avoiding continuous cropping, even if normally planting Also will appear under the conditions of training management growing way die down, the increase of soil pest and disease damage, soil nutrient numerous imbalances, soil physico-chemical property dislike Change, soil enzyme activities changes, crop is produced from poison.Locomotive engine agricultural technology Co., Ltd, Henan Province develops out one kind The problem of can solve the anti-continuous cropping in watermelon soil, therefore carry out watermelon in the Shangqiu City Xiayi County town Hui Ting within 2019 and apply anti-continuous cropping soil The test of earth renovation agent, profile test and measured result are summarized as follows:

One, test situation

1. test site

Watermelon application " anti-continuous cropping soil renovation agent " test site is arranged in Henan Province, the town Hui Ting, Shangqiu City Xiayi County, soil types For light loam.

2. material to be tested

It is " 8424 ", greenhouse gardening for examination variety of watermelon.For examination " anti-continuous cropping soil renovation agent " by Henan Province's locomotive engine agricultural skill Art Co., Ltd development & production simultaneously provides.

3. experimental design

Single factor test cell control methods is taken in watermelon application " anti-continuous cropping soil renovation agent " test, that is, sets 2 processing: processing 1(is not Application, that is, compare, code CK) and processing 2(application, code AS), 3 repetitions are arranged in each processing.

Two, production method is surveyed

In test test area, control by several times picks mature watermelon with application " anti-continuous cropping soil renovation agent ", real to produce It is paid, while watermelon saccharinity is measured with hand-held saccharous detector, depth of soil and soil density are measured with soil density instrument.

Three, the increasing yield and improving quality effect of watermelon garden application " anti-continuous cropping soil renovation agent "

The influence of " 1. anti-continuous cropping soil renovation agent " to watermelon diseased plant number

As a result, the diseased plant number of watermelon application " anti-continuous cropping soil renovation agent " processing has 2 plants, diseased plant rate is live actual observation 6.7%；The diseased plant number of control has 10 plants, and diseased plant rate 11.1%, the diseased plant rate of application relatively control reduces by 4.4 percentage points.

The influence of " 2. anti-continuous cropping soil renovation agent " to the yield of watermelon

Practical survey produces as a result, " anti-continuous cropping soil reparation is applied in watermelon garden under identical production, ecology, kind, edaphic condition Agent ", average each 3.4 kg of the yield of watermelon, cell average product are 90.87 kg；The average single melon of control weighs 3.88 kg, cell Average product is 121.67 kg, and application increases by 0.48 kg than compareing single melon again, and cell increases production 30.8 kg, and rate of growth is 33.89 %。

The influence of " 3. anti-continuous cropping soil renovation agent " to watermelon saccharinity

With hand-held saccharous detector scene practical measurement result, watermelon applies " anti-continuous cropping soil renovation agent ", sugar content average out to 10.28 %(n ﹦ 10), compare watermelon saccharinity average out to 9.45%(n ﹦ 10), sugar content application relatively control increases by 0.83 percentage point.

The influence of " 4. anti-continuous cropping soil renovation agent " to melonry soil density

With soil density instrument scene practical measurement result, under conditions of identical topsoil depth, watermelon applies " anti-continuous cropping soil Renovation agent ", 15.69 kg/cm of soil density average out to², compare 17.34 kg/cm of average out to², application relatively control reduction 1.65 kg/cm², the amplitude that reduces is 9.52%.

Four, conclusion

In summary result illustrates, after continuous cropping greenhouse watermelon applies anti-continuous cropping soil renovation agent, diseased plant rate reduces by 4.4 percentages Point, cell watermelon average weight increase by 30.8 kg than control, and increment rate is 33.89 %, and sugar content increases by 0.83 percentage point, Under the conditions of identical topsoil, soil density reduces by 1.65 kg/cm², the amplitude that reduces is 9.52%.

Test 2: the application effect for implementing Table Grape application " anti-continuous cropping soil renovation agent " to the present invention is reported:

One, exemplary scenario:

1. place of demonstrating: Table Grape application " anti-continuous cropping soil renovation agent " demonstration place is arranged in Shangqiu City Zhecheng County, Henan Province Qi great Zhuan Cunru poem home farm, great favour township (N34 ° 02 ' 5.44 of north latitude ", east longitude E115 ° 26 ' 45.96 "), soil types is tide Heavy loamy texture soil in native subclass.

2. material to be tested: it is seedless type " early honey " for examination grape variety, the age of tree 5 years, for 7 mu of vineyard area of examination. Material to be tested is " anti-continuous cropping soil renovation agent " of the invention.

3. exemplary design: single factor test great Qu control methods is taken in Table Grape application " anti-continuous cropping soil renovation agent " demonstration, Set 2 processing: processing 1(is not applied, that is, is compareed, code CK) and processing 2(application, code AS), in the demonstration area of setting Interior, two processing are 9 row areas.

4. other management: " anti-continuous cropping soil renovation agent " of the invention is spread fertilizer over the fields around trunk, at any time earthing, amount of application 2kg/ mus.Other agronomic measures are identical, and Ji Mushi compound fertilizer (14-16-15s) 50kg, organic fertilizer 80kg make base manure；Berry expands Phase and coloring phase, mu applied liquid fertilizer 10kg, potassium dihydrogen phosphate 1kg respectively.Prevent and treat pest and disease damage, early period Mancozeb and stone sulphur close Agent, later period add foliar fertilizer with the odd positive Traditional Chinese medicine fertilizer of standing grain, and discovery has downy mildew to reveal plus Du Pont gram, and insect prevention is wide with Du Pont's health.In addition, Berry expansion period combines watering punching to apply biogas liquid fertilizer twice.

Two, survey production method: in experiment and demonstration region, control (CK) and application " anti-continuous cropping soil renovation agent " (AS) with Machine respectively selects two rows, each 20 plants of selection, carries out fruit ear observation and paid meter produces, while measuring berry sugar content with hand-held saccharous detector Deng.

Three, the increasing yield and improving quality effect of vineyard application " anti-continuous cropping soil renovation agent ":

The influence of " 1. anti-continuous cropping soil renovation agent " to the effective spike number of grape: live actual observation is as a result, Table Grape is applied " anti-continuous cropping soil renovation agent " processing, 20 plants of effective fruit ears add up to 174 fringes, and average every plant of 8.7 fringes do not apply " anti-continuous cropping soil Earth renovation agent " processing (compare), 20 plants of effective fruit ears add up to 152 fringes, and be averaged every plant of 7.6 fringes, identical soil, ecology, Under kind, management condition, application " anti-continuous cropping soil renovation agent " relatively compares effective fruit ear and increases by 22 fringes, and amplification 14.47% is average Every plant of effective fruit ear increases by 1.1 fringes, 14.47 % of amplification.

The influence of " 2. anti-continuous cropping soil renovation agent " to Table Grape yield: practical survey produces as a result, in identical production, life Under state, kind, edaphic condition, " anti-continuous cropping soil renovation agent " is applied in vineyard, 20 plants of Table Grape yield 161.74kg, average Every plant of Table Grape yield 8.087kg converts into per mu yield Table Grape 1798.01kg, relatively compares per mu yield Table Grape 1314.66kg Increase production 480.35kg, amount of increase in production is up to 36.76%.If calculated by locality 12 yuan/kg of Table Grape average sales price, Table Grape Garden increases 2kg " anti-continuous cropping soil renovation agent " per acre, and average yield per mu value increases by 5764.2 yuan, remarkable in economical benefits.

The influence of " 3. anti-continuous cropping soil renovation agent " to Table Grape sugar content: using hand-held saccharous detector scene practical measurement knot Fruit, Table Grape apply " anti-continuous cropping soil renovation agent ", 16.86 %(n ﹦ 5 of berry sugar content average out to), compare berry sugar content 15.76 %(n ﹦ 5 of average out to), berry sugar content application relatively control increases by 1.1 percentage points, improves 6.98% relatively.

Four, conclusion: under the influence of vineyard is by extreme cold air, the grape for applying anti-continuous cropping soil renovation agent is effective Fruit ear increases by 22 fringes, amplification 14.47%, and average every plant of effective fruit ear increases by 1.1 fringes, amplification 14.47%, and sugar content increases by 1.1 Percentage point, per mu yield relatively control increase 480.35kg, and average yield per mu value increases by 5764.2 yuan, and remarkable in economical benefits illustrates anti-heavy The resistance of grape can be improved in stubble soil-repairing agent, increases the yield of grape, improves the sugariness of grape, reach increasing yield and improving quality Effect.(rear attached expert's signature table)

Test 3: anti-continuous cropping soil renovation agent is reported in Yunnan-Guizhou Hunan Yu Sisheng tobacco producing region flue-cured tobacco application effect:

One, Yu Sisheng cigarette district in Yunnan-Guizhou Hunan applies anti-continuous cropping soil renovation agent overview:

1, application method designs: each province takes unified single factor test field comparative's method to be designed using basic point, that is, sets control Anti- continuous cropping soil renovation agent (code AS) two processing of (not having to anti-continuous cropping soil renovation agent, code CK) and application.Anti- continuous cropping soil Earth renovation agent dosage 1kg per acre, cave is applied when tobacco leaf transplanting.Basic (the kind of fertilizer of the fertilising of various chemical fertilizer and organic fertilizer at two Class and quantity) it is corresponding consistent, i.e. each point fertilising (bottom, top dressing) is applied as throughout the year conventional under respective production, ecology, technical conditions respectively Fertile standard is implemented, and other field management measures are consistent.20-40 mus of each basic point processing area, the flue-cured tobacco cultivars of plantation are mostly cloud and mist Yun yan85, cloud and mist 87, cloud and mist 99, cloud and mist 105 and K326, Zhongyan-100, the Qin's cigarette 96 etc. of series, field planting spacing is more For 50cm × 120cm, planting density is generally at 1000-1100 plants/acre or so.

2, using tobacco field ecological setting: various regions soil types is related to red soil, yellow earth, brown earth, yellowish soil, tide from south to north Soil, cinnamon soil etc.；Topography and geomorphology has mountainous region, has Plain, also has hills zone of transition, and height above sea level difference is also very big, from deficiency 100 meters to more than 1000 meters or even 2000 meters or more；Application region span is big, is related to Southwest tobacco-growing areas (Yunnan-Guizhou), cigarette district (Hunan, Central China West), North China cigarette district (Henan), 8 latitudes are crossed in cigarette district physical geography position from south to north, i.e., from 26 ° of north latitude or so up to 34 ° Left and right, for west and east span also from 114 ° of east longitude or so to 102 ° or so, natural ecology weather conditions have very big difference.Specified place one As be selected in that vega soil fertility is better-than-average, soil property is good, has the old cigarette district for preferably filling row's condition, tobacco grower has cultivation skill abundant Art experience and perfect tobacco flue-curing facility.

3, using observation and analysis method: during each base development of flue-cured tobacco, all every processing of economical character observation are equal By 5 point samplings, every sampling point random continuous chooses representative 10 plants of cigarette strain, observes its plant height, effective blade number, blade respectively Maximum length and the economical characters such as width, observe the degree of packing index of cigarette strain incidence and topsoil soils；Tobacco leaf is adopted by several times Every processing determines 100~110 plants of samples at random when plucking, and weighs the fresh weight of all picking blades respectively, marks to it laggard Row baking；10 plants of stalks and its 0~20 subsidiary cm topsoil root mass are selected in every processing after picking by several times, use clear water in time Laboratory is taken back after rinsing well carries out stalk and the fresh of root system, dry weight observation respectively；After the tobacco flue-curing picked to gradation again The dry weight for weighing sample blade respectively, counts per mu yield, while carrying out flue-cured tobacco grade classification together；Finally produced according to flue-cured tobacco Amount, the output value, income technical parameter, which calculate, to be produced, throws ratio, and Economic and Efficiency Analysis is carried out.

Two, flue-cured tobacco is using anti-continuous cropping soil renovation agent result and analysis

1. influence of the anti-continuous cropping soil renovation agent to flue-cured tobacco Other Main Agronomic Characters

(1) promotion effect of the anti-continuous cropping soil renovation agent to tobacco plant field height.

Various regions observed result is included in table 1 respectively.As seen from Table 1, flue-cured tobacco apply anti-continuous cropping soil renovation agent with compare Compare, Yunnan-Guizhou Hunan various regions cigarette district tobacco leaf plant height increases separately 3.6~45.5cm(and is averaged 18.0cm), amplification is respectively 3.6%~ 63.6%(is average 21.0%), 2) tobacco leaf stalk dry matter weight amplification is shown in Table up to 12.5%(.Show it is existing production, ecology, Under the conditions of technology, kind, flue-cured tobacco promotes the growth and development of plant stalk using anti-continuous cropping soil renovation agent, mainly passes through for flue-cured tobacco Growth, development or even the tobacco leaf volume increase and quality improving etc. of Ji organ blade provide important supporting substances.

1 Yunnan-Guizhou Hunan cigarette district of table applies influence of the anti-continuous cropping soil renovation agent to flue-cured tobacco plant height

Dry, fresh weight the influence (the Zunyi City, Guizhou Province area Bo Zhou Leshan Technology Park) to tobacco leaf plant stalk of table 2

(2) promotion effect of the anti-continuous cropping soil renovation agent to the economic organ blade of tobacco.

3 Yunnan-Guizhou Hunan cigarette district of table applies influence of the anti-continuous cropping soil renovation agent to Flue-cured growth and development

Tobacco leaf is the major organs to form economic flow rate, in the metastable situation of unit area tobacco plant group, Although variant between kind, the quantity and its leaf area size of single plant effective blade be obtain tobacco production it is decisive because Element also has substantial connection with cigarette quality superiority and inferiority.The result shows that (table 3), the anti-continuous cropping soil renovation agent of tobacco use is raw to blade The facilitation trend of long development is all consistent, and effect is it will be evident that different cigarette districts, different cultivars, different soils, different lifes State weather conditions are unexceptional.Anti- continuous cropping soil renovation agent is increased by promoting effect to effective blade and its leaf area for tobacco It produces and improving quality provides important material base.

(3) influence of the anti-continuous cropping soil renovation agent to flue-cured tobacco root growth and development.The main function of anti-continuous cropping soil renovation agent One of energy is exactly to remarkably promote root system while improvement soil penetrating situation to increase and extend.Table 4 as it can be seen that no matter southwestern cigarette Area or Henan tobacco region, after the anti-continuous cropping soil renovation agent of tobacco use, tobacco field topsoil, root system fresh weight, dry weight are dramatically increased, Its amplification is respectively that 7.4%~19.3%(is average 14.1%), 14.4%~28.6%(is average 23.1%), wherein again with dry weight It is more significant to increase effect.

Dry, fresh weight the influence to tobacco field topsoil root system of the anti-continuous cropping soil renovation agent of table 4

2. influence of the anti-continuous cropping soil renovation agent to flue-cured tobacco disease resistance

Continuous due to working condition improves and complicated and changeable, certain cigarette district (such as high and cold mountains in addition of tobacco field ecology miniclimate Ground etc.) it is difficult to the continuous cropping factor regulated and controled, each main cigarette district tobacco diseases in China have the tendency that more and more heavy.In flue-cured tobacco using anti-heavy During stubble soil-repairing agent, the observation compared with system is carried out to its disease resistance, has further demonstrated anti-continuous cropping soil renovation agent Significant disease resisting effect is shown in Table 5,6 respectively.Tobacco mosaic disease rate declines 2~58.7 percentage points, relative drop amplitude 15.4%~96.5%；6~30 percentage points of tobacco black shank diseased plant rate decline, relative drop amplitude 16.3%~65.2%, cloud The level ground Nan Hualu village's observation point is even up to 100%, effect highly significant.

Influence of the anti-continuous cropping soil renovation agent of table 5 to tobacco mosaic disease disease incidence

Influence of the anti-continuous cropping soil renovation agent of table 6 to tobacco black shank disease incidence

3. influence of the anti-continuous cropping soil renovation agent to the elastic situation of soil

7 Yunnan-Guizhou Hunan cigarette district of table applies influence of the anti-continuous cropping soil renovation agent to vega soil hardness

Each cigarette district multipoint system field scene uses soil hardness analyzer and soil density analyzer measurement result respectively It confirms (table 7), after Yunnan-Guizhou Hunan cigarette district each point tobacco field applies anti-continuous cropping soil renovation agent, fathoms range in certain soil layer Interior, soil hardness averagely declines 45N/cm compared with the control for application², average fall 10.8%.It is converted into measurement range Every cm soil layer pressure, 0.59 ~ 5.88 N/cm of reduction of the ratio control of Yunnan-Guizhou Hunan application.Anti- continuous cropping soil renovation agent is in very great Cheng The elastic situation that tobacco plant root soil is improved on degree is conducive to the growth and development of root system and to Directional Extension in length and breadth.

Each cigarette district in 8 Henan Province of table applies influence of the anti-continuous cropping soil renovation agent to the vega soil degree of packing

4. anti-continuous cropping soil renovation agent comprehensive resistance new discovery

(1) anti-continuous cropping soil renovation agent has anti-waterflooding function.In western Hunan flue-cured tobacco growing district Longshan County big Anxiang five-pointed star village (north latitude N29 ° 30 ' 15.54 ", east longitude E109 ° 39 ' 42.49 ") there was a pouring rain, and there is ponding situation in tobacco field.Field observation discovery, There is notable difference between the upgrowth situation processing of No. 2 demonstration plots Flue-cureds, in control treatment area, the blade of plant base portion Withered and yellow or even withered fallen leaves it is more, while normal green blade is also fewer, and applies anti-continuous cropping soil renovation agent processing Qu Ze is on the contrary, not only the withered and yellow blade of plant base portion relatively compares reduction 27.5%, but also normal green blade is but than control increasing Add 24.1%.Anti- continuous cropping soil renovation agent plot is applied, due to the improvement of the penetrating situation of soil, correspondingly root system development is being substantially just Often, organic synthesis is more, and consumption is few, so improving the growth quality of aerial part stalk and blade, counteracts waterflooding in more days Bad ecology influence.

(2) applied nitrogen adverse effect on the high side can be eliminated or buffered using anti-continuous cropping soil renovation agent.In Yunnan Province The Zhaotong City Ludian County village Utopia Tuo Gu (N27 ° 10 ' 30.53 of north latitude ", east longitude E103 ° 36 ' 22.88 ") anti-continuous cropping soil of application Earth renovation agent point is observed, and the tobacco field inferior leads and a part of middle leaf had picked end and carried out normal a heatable brick bed cigarette at that time, It waits picking baking there are also upper leaf.Observation as a result, appropriate using anti-continuous cropping soil renovation agent plot tobacco plant blade thickness, at Ripe to fall Huang Zhengchang, field tobacco plant population growth and development is consistent, and robust plant, diseased plant is few, and especially Maturity of Tobacco Leaf is high, Leaf color is good, and blade is vivid glossy, and hand touches blade and feels oil foot, and adjacent does not apply anti-continuous cropping soil renovation agent plot, cigarette Field plant population has prosperous growth trend, and leaf color is gloomy, and remaining green when it is due to become yellow and ripe ripe lateral bud late is more, and Field diseased plant rate, sick leaf rate are on the high side, bright It is aobvious to be seen to be that amount of application of nitrogen fertilizer is bigger than normal or nitrogenous fertilizer imposes result caused by evening partially.Anti- continuous cropping soil renovation agent has certain elimination or slow Rush the unreasonable effect for leading to negative effect of nitrogen application.

5. the increasing yield and improving quality effect that flue-cured tobacco applies anti-continuous cropping soil renovation agent

9 Yunnan-Guizhou Hunan cigarette district of table applies influence of the anti-continuous cropping soil renovation agent to yield of flue-cured tobacco, grade and economic benefit

Systematic observation after tobacco flue-curing the result shows that, it is relatively more aobvious that Yunnan-Guizhou Hunan cigarette district flue-cured tobacco applies anti-continuous cropping soil renovation agent volume increase It writes, per unit area yield amount of increase in production 2.5%~10.9%, averagely increases production 6.3%, first-class cigarette ratio is opposite to improve 11.4%, and per mu yield value is average Increase by 461.82 yuan, based on anti-40 yuan of continuous cropping soil renovation agent sale price per kilogram, produces, throws than average out to 11.55.

6. flue-cured tobacco reduces the active effect of heavy metal ion using anti-continuous cropping soil renovation agent

After the tobacco leaf of Guizhou Province Yan Keyuan Pingba proving ground applies anti-continuous cropping soil renovation agent, heavy metal cadmium in tobacco leaf is contained Amount is detected, and obtain result (table 10): from the point of view of the detection of cured tobacco leaf cadmium, applying anti-continuous cropping soil renovation agent can effectively drop The content of low tobacco leaf cadmium, under tobacco leaf, in, top content is respectively than compareing low 0.17mg/kg, 0.72 mg/kg, 0.48mg/kg.

10 Guizhou Province's Yan Keyuan Pingba proving ground tobacco leaf of table is applied anti-continuous cropping soil renovation agent and is contained to cadmium in cured tobacco leaf The influence (unit: mg/kg) of amount

Position	CK	AS	AS-CK	Reduction amplitude/%
					Lower part cigarette	2.73	2.55	-0.17	6.23
Middle part cigarette	2.12	1.41	-0.72	33.96
					Upper smoke	2.05	1.56	-0.48	23.41

Three, the anti-continuous cropping soil renovation agent conclusion of flue-cured tobacco Demonstration Application: flue-cured tobacco has significant and comprehensive using anti-continuous cropping soil renovation agent The promotion tobacco growing of conjunction is developed, disease-resistant degeneration-resistant, chesson, improving quality, is increased economic efficiency, reducing weight in Flue-cured The effect of tenor.

1. more fully promoting the growth and development of flue-cured tobacco stalk, blade, root system, tobacco plant ground, underground active development Stalwartness is coordinated, and mutually promotes, provides material base for yield composition.

2. enhancing tobacco plant to the disease resistance of tobacco leaf balck shank and mosaic disease, growth phase is the improvement of later baking properties Fundamental role is played, due to the spy that is nontoxic, harmless, pollution-free, adapting to wide (broad spectrum activity) of anti-continuous cropping soil renovation agent Distinguished service energy provides a new way for tobacco non-polluted cultivation, green cultivation, high benefit cultivation, rehabilitating soil cultivation.

3. being effectively improved for flue-cured tobacco economical character also improves soil plate with one of the major function of anti-continuous cropping soil renovation agent Knot, enhancing soil permeability can have direct or indirect relationship, for new, the old cigarette district vega soil ecological environment of improvement and differently The robust techniques guarantee of shape landforms, providing the foundation property of land occupation condition field microclimate, duration, novelty complicated and changeable.

4. anti-continuous cropping soil renovation agent can significantly improve flue-cured tobacco grade, yield not only can be improved, but also can improve Quality optimizes vega soil ecological environment, negatively affects caused by elimination nitrogen application is improper, amount of increase in production 6% ~ 23.5%, increases 493.36 ~ 1181.4 yuan of value, amplitude 11.7% ~ 41.7% of rising in value produces throwing ratio and commonly reaches 12.33 ~ 29.79:1.5. applying anti-heavy Stubble soil-repairing agent can be substantially reduced the content of heavy metal cadmium in cured tobacco leaf, so that cadmium in middle part and top cured tobacco leaf Content reduces by 2.5% ~ 52.2%, 6.7% ~ 34.7% respectively.

Test 4: the effect of watermelon application " anti-continuous cropping soil renovation agent ", which is surveyed, produces report:

When due to planting watermelon or close relative edge crop in same soil for a long time, it just will appear continuous cropping phenomenon.And watermelon is A kind of plant for most avoiding continuous cropping, will appear under the conditions of normal cultivation management growing way die down, soil pest and disease damage increase, Soil nutrient numerous imbalances, soil physico-chemical property deteriorates, soil enzyme activities changes, crop is produced from poison.In Suiyang District Lee The inner Yang Jinhang watermelon in mouth town five applies the test of anti-continuous cropping soil renovation agent, and profile test and survey produce result and be summarized as follows:

One, test situation:

1. test site: being located at the inner poplar in Henan Province, the town Li Kou, Shangqiu City Suiyang District five, soil types is light loam.

2. material to be tested: being " surmounting dream ", greenhouse gardening for examination variety of watermelon.

3. experimental design: single factor test cell control methods is taken in test, if 2 processing: processing 1(is not applied, that is, is compareed, Code CK) and processing 2(application, code AS), 3 repetitions are arranged in each processing, and each plot area is the ㎡ of 3m × 8m=24.

4. other management: about 3 side of conventional treatment bottom application cow dung, nitre sulfenyl 45%, 15-15-15 compound fertilizer 50 kg per acre.

Two, survey production method: in test test area, control (CK) and application " anti-continuous cropping soil renovation agent " (AS) are by several times Mature watermelon is picked, real production is paid, while measuring watermelon saccharinity etc. with hand-held saccharous detector.

The influence of " 1. anti-continuous cropping soil renovation agent " to watermelon diseased plant number: live actual observation is as a result, watermelon application " anti-continuous cropping soil Earth renovation agent " processing, a total of 181 plants of 3 repetitions, wherein 10 plants of diseased plant number, 2 plants of malformed fruit；A total of 184 plants of control, Wherein 2 plants of diseased plant number, 1 plant of malformed fruit, diseased plant rate reduces by 4.4 percentage points, and fruit deformation rate reduces by 0.6 percentage point.

The influence of " 2. anti-continuous cropping soil renovation agent " to the yield of watermelon: it is practical survey produce as a result, identical production, ecology, Under kind, edaphic condition, " anti-continuous cropping soil renovation agent " is applied in watermelon garden, and picking 66 watermelon weight is 138.5kg, average every A the yield of watermelon 2.1kg picks watermelon gross weight 356.6kg twice, compares 51 the yield of watermelon 90.3kg, and it is total to pick watermelon twice Weight 458.3kg, averagely each the yield of watermelon 1.8kg, single watermelon increase by 0.3 kg, 101.7 kg of increasing yield of water melons, and rate of growth is 28.5%。

The influence of " 3. anti-continuous cropping soil renovation agent " to watermelon saccharinity: hand-held saccharous detector scene practical measurement result is used, Watermelon applies " anti-continuous cropping soil renovation agent ", 10.13 %(n ﹦ 6 of sugar content average out to), compare watermelon saccharinity average out to 9.75%(n ﹦ 6), sugar content application relatively control increases by 0.38 percentage point, improves 3.90% relatively.

Four, conclusion: after continuous cropping greenhouse watermelon applies anti-continuous cropping soil renovation agent, diseased plant rate reduces by 4.4 percentage points, abnormal fruit Rate reduces by 0.6 percentage point, picks watermelon total weight twice than control and increases by 101.7 kg, increment rate is 28.5 %, sugar content Increase by 0.38 percentage point, opposite raising 3.90%.

Test the fertilizer efficiency demonstration of 5: Mouping County, shandong Province area 2017-2018 anti-continuous cropping soil renovation agent compounding application on apple Final report:

1 demonstration carrys out source and destination

Increase for the anti-continuous cropping soil renovation agent of verifying Henan Province's locomotive engine agricultural technology Co., Ltd production in weight-reducing volume increase, upgrading The effect of effect, improvement soil etc., provides foundation to be widely applied, Yantai City, clay fertilizer station, Mouping District was in 2017 10 The moon has carried out field demonstration in October, 2018 on apple.

2 exemplary materials and method

2.1 for trying soil

Exemplary arrangement is in Yantai City, the town Ju Gezhuan, the Mouping District village the QuinceLuo orchard Zhao Shuhai, soil types brown earth, the light earth of surface layer quality, Fertility is uniform, and irrigation condition is good, does not do fertilizer test.It demonstrates and preceding 18 days October in 2017 physical and chemical property has been carried out to demonstration Shape measurement, it is as a result as follows: organic matter 10.8g/kg, full nitrogen 0.74g/kg, available phosphorus 53.7mg/kg, available potassium 195mg/kg, pH Value 5.0, bulk density 1.44g/cm3.

2.2 for trying fertilizer

Anti- continuous cropping soil renovation agent is provided by locomotive engine agricultural technology Co., Ltd, Henan Province.

2.3 for studying object

Apple, kind Fuji apple (bagging), life in 12 years, 50 plants per acre.

2.4 exemplary designs and method

2.4.1 exemplary design

Demonstration sets 3 processing.

Processing 1: local habits fertilization (CK)；

Handle the anti-continuous cropping soil renovation agent of 2:CK+；

2.4.2 demonstration methods

Processing 1 applies 45% organic fertilizer 375kg, compound fertilizer (20-10-15) 80kg in mu base on November 3rd, 2017, and base manure is used and put Penetrate shape ditch spread；The mu on the 21st of August in 2018 imposes Water soluble fertilizer (15-5-30) 10kg, using spreading fertilizer over the fields the border irrigation behind ground.Processing 2 in On the same day mu base apply the anti-continuous cropping soil renovation agent of locomotive engine organic fertilizer 375kg+2 kg (being collectively referred to as anti-continuous cropping soil renovation agent, similarly hereinafter), Formula fertilizer (17-13-15) 64kg, base manure use radial ditch spread；The mu on the 21st of August in 2018 imposes Water soluble fertilizer (15-5-30) 8kg, using spreading fertilizer over the fields the border irrigation behind ground.Processing 3 applies anti-continuous cropping soil renovation agent 375kg, Water soluble fertilizer (20-12- in mu base on the same day 18) 30kg, mu on June 13rd, 2018 apply Water soluble fertilizer (15-30-5) 10kg, August mu on the 21st applies Water soluble fertilizer (15-5-30) 10kg, Organic fertilizer uses radial ditch spread, and chemical fertilizer is all made of fertigation.Each processing is other than above-mentioned difference, other agronomic measures and field Between manage it is consistent.

3 demonstration results and analysis

Influence of 3.1 different disposals to the soil weight

We have carried out the measurement of topsoil soils bulk density to different disposal respectively for September in 2018 29 days, as a result see (table 1).By table In obtain, processing 2 than processing 1 Volume-weight Determination values reduce 0.09 g/cm³, illustrate that anti-continuous cropping soil renovation agent has loose soil The effect of earth.

Different disposal topsoil soils Volume-weight Determination value unit after table 1 is demonstrated: g/cm³

Processing	Bulk density	It is reduced than CK
			Handle 1(CK)	1.44	-
Processing 2	1.40	0.04

Influence of 3.2 different disposals to crop root

September in 2018 29 days we comparison of uprooting has been done to different disposal apple tree underground root system.Distinguishing at 1 meter of trunk Dig long 1 meter, wide 50 centimetres and deep 50 centimetres of soil layer, it can be seen that handle 2 lateral root it is healthy and strong, more, bifurcated is more, and capillary root is sent out Reach, handle 1 lateral root it is obviously weak, lateral root is relatively tiny, and bifurcated is few, and capillary root is few.Illustrate to apply anti-continuous cropping soil renovation agent, It can promote the development of root system of the apple.

Influence of 3.3 different disposals to crop yield and the output value

3.3.1 influence of the different disposal to crop yield

When survey production, we measure 3 surveys production cells at each handle at random, each survey production 6 plants of apple trees of cell, and Yu Shiyue 10 days one Secondary property harvest, each cell list is received, list claims, Dan Jichong, counts each cell production, yield result is shown in Table 2.The results show that anti-heavy Stubble soil-repairing agent increases production 965 kg/ mus of apple compared with habits fertilization, rate of growth 31.7%.Analysis of variance, the > of F=36.08 F0.01=10.90, difference reaches extremely significant level between processing.

2 apple production result table of table

Through Multiple range test, volume variance reaches the level of signifiance between processing 1 and processing 2.In this test, anti-continuous cropping soil reparation Agent is extremely significant compared with habits fertilization volume increase, illustrates to apply anti-continuous cropping soil renovation agent energy chesson, promotes root system of the apple development, changes The nutrient environment of apple root layer has been apt to it, has been conducive to root system and absorbs more nutrition, to increase apple production, effect of increasing production is extremely aobvious It writes.

3 different disposal yield result Multiple range test table of table

3.3.2 influence of the different disposal to the crop output value

It applies anti-continuous cropping soil renovation agent apple and is relatively all substantially better than control to impinging upon apple color, fruit size etc., disease Relatively control is also opposite in terms of insect pest is reduced.We randomly select 3 plants of apple trees at each processing, have carried out apple output value constituent element Investigation.As the result is shown: applying anti-continuous cropping soil renovation agent than control single plant fruiting amount and increase 73, diseased fruit rate reduces 5.3%, I and II fruit rate increases 5%, and the output value increases 6598 yuan/mu (see Table 4 for details).

4 different disposal apple output value constituent element summary sheet of table

Note: I and II fruit is counted according to average 7 yuan/kg, remaining fruit according to average 2 yuan/kg.

3.4 different disposals are to increasing production and improying productivity and the influence of input-output ratio

Under conditions of field management is consistent, if only considering to apply with the cost for testing related fertilizer and fertilizing, watering Anti- continuous cropping soil renovation agent is total to more 109.4 yuan/mu of input cost, only than 150 yuan/mu of the increased costs that habits fertilization is put into 6588.6 yuan/mu of volume increase value, see Table 5 for details by input-output ratio 1:59.3().

5 different disposal Economic and Efficiency Analysis unit of table: member/mu

4 demonstration conclusions

The anti-continuous cropping soil renovation agent of locomotive engine agricultural technology Co., Ltd, Henan Province production can reduce the soil weight, has and dredges The effect of mellow soil；It can promote the development of root system of the apple, root system is healthy and strong, capillary root is more；Apple 965kg/ mus of volume increase, rate of growth It is 31.7%, volume increase reaches extremely significant level；It is all substantially better than in terms of apple color, fruit size, apple pair According to；The output value increases 6598 yuan/mu, has a net increase of 6488.6 yuan/mu of the output value, input-output ratio 1:59.3.In short, Henan Province's locomotive engine The anti-continuous cropping soil renovation agent of agricultural technology Co., Ltd production loses weight volume increase, upgrading synergy, improvement soil etc. just on apple The significant effect in face, it is proposed that be widely applied.(rear attached expert's signature table).

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Claims

1. a kind of bacillus subtilis antagonistic strain Z-15, which is characterized in that the classification of bacillus subtilis antagonistic strain Z-15 Learning scientific name is bacillus subtilis (Bacillus subtilis), is preserved in the micro- life in Guangdong Province on May 5th, 2019 Object Culture Collection Center, address are 5 building, No. 59 building of Xianlie Middle Road, Guangzhou City 100, and culture presevation number is GDMCC NO: 60653。

2. a kind of anti-continuous cropping soil renovation agent made using bacillus subtilis antagonistic strain Z-15 described in claim 1, It is characterized in that, which includes that following sample is made in parts by weight:

3. anti-continuous cropping soil renovation agent according to claim 2, which is characterized in that the anti-continuous cropping soil repairs dosage form State is solid-state.

4. anti-continuous cropping soil renovation agent according to claim 2, which is characterized in that the solid support medium refers to containing thick The solid support medium of fat, be with the sesame cake fertilizer of pure raw sesame cake fermentation maturity, crude fat weight percentage >=5%, Organic matter weight percentage >=50%, moisture weight percentage composition≤20%, pH 5.5 ~ 8.5 under the conditions of this, give withered grass bud Spore bacillus antagonism strain Z-15 creates the supply of special better living environment and nutrition, is more advantageous to the breeding of the strain.

5. anti-continuous cropping soil renovation agent according to claim 2, which is characterized in that fatty alcohol polyoxyethylene ether is to use hydrogen Sodium oxide molybdena makees catalyst, and with ethylene oxide ring-opening polymerization occurs for long-chain fatty alcohol in the presence of anhydrous and no oxygen It is made.

6. anti-continuous cropping soil renovation agent according to claim 2, which is characterized in that fatty acid cane sugar ester is at 40~45 DEG C At a temperature of, it is generated by sucrose and fatty acid through esterification.

7. a kind of preparation method of anti-continuous cropping soil renovation agent as claimed in claim 2, which is characterized in that specific production step It is rapid as follows:

Solid support medium of the step 3) containing crude fat is that fermentation liquid is added after pure raw sesame cake crushes, 50 are increased in temperature~ After 65 DEG C, stir stirring, aeration work, fermented and manufactured, high temperature is mainly for effectively killing the disease in sample Bacterium worm's ovum, and large quantity of moisture is evaporated, pay special attention to ferment hot stage when temperature reaches 65 DEG C or so in mid-term, it is timely Turning is unlikely to heap temperature more than 70 DEG C, so that crude fat weight percentage >=5% in the sesame cake fertilizer fermented, organic Matter weight percentage >=50%, moisture weight percentage composition≤20%, pH 5.5 ~ 8.5 under the conditions of this, give bacillus subtilis Bacterium antagonism strain Z-15 creates the supply of special better living environment and nutrition, is more advantageous to the breeding of the strain；

Under the conditions of temperature is 32~36 DEG C, 6.5 ~ 7.0 pH, bacillus subtilis antagonistic strain Z-15 is being cultivated step 4) 48h is cultivated on base；

It is good that cultured bacillus subtilis antagonistic strain Z-15 and bacillusmusilaginosiengineering are placed on fermentation maturity by step 7) It is blended in solid support medium containing crude fat, solid support medium refers to solid support medium containing crude fat, in particular to pure life The sesame cake fertilizer of sesame cake fermentation maturity, crude fat weight percentage >=5% of sesame cake fertilizer, organic matter weight percent contain >=50%, moisture weight percentage composition≤20%, pH 5.5 ~ 8.5 are measured, is bred in the environment of adaptation, for use；

8. the preparation method of anti-continuous cropping soil renovation agent according to claim 7, which is characterized in that the withered grass bud Spore bacillus antagonistic strain Z-15 be by my company and Agricultural University Of Hebei's Life Science College cooperative research and development, it is anti-with wide spectrum Bacterium activity and extremely strong anti-adversity ability can secrete a variety of antibiotic and mould substance, stimulate the unfavorable growth ring of harmful microbe Border, prevention and treatment soil-borne disease, the growth or propagation for inhibiting harmful pathogen, resistivity of the enhancing plant to pathogen.

9. the preparation method of anti-continuous cropping soil renovation agent according to claim 7, which is characterized in that effective work Bacterium number >=2.0 hundred million/g.