CN102240664A - Method for restoring soil polluted by manganese - Google Patents
Method for restoring soil polluted by manganese Download PDFInfo
- Publication number
- CN102240664A CN102240664A CN2011101212988A CN201110121298A CN102240664A CN 102240664 A CN102240664 A CN 102240664A CN 2011101212988 A CN2011101212988 A CN 2011101212988A CN 201110121298 A CN201110121298 A CN 201110121298A CN 102240664 A CN102240664 A CN 102240664A
- Authority
- CN
- China
- Prior art keywords
- parts
- manganese
- soil
- culture medium
- adopts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a method for restoring soil polluted by manganese. A bacterial strain with strong manganese resistance is sorted from rhizosphere (2mm around root) soil of castor-oil plant grown in a manganese mine polluted area. After sorting a bacterial strain with strong tolerance and adsorption capability to manganese, through biology fermentation technology, bacterial manure is prepared. According to the method, phytolacca americana is planted in an alkaline manganese mine tailings stacking area, the bacterial manure and a mixed fertilizer are spread into a plant pit first in planting, after uniform mixing of the bacterial manure and the mixed fertilizer phytolacca americana seeds are sowed into the plant pit and followed by covering with soil. In this way, the phytolacca americana grows fast in the manganese mine tailings stacking area, a plurality of phytolacca americana plants are obtained in a short time. After a first time artificial rearing, repeated sowing is not needed, and heavy metal pollution in the manganese mine area is absorbed and controlled. The method can be used for ecology restoration of a degenerated ecology system of a mine, greening of a mining area and the like.
Description
Technical field
The present invention relates to biological field, particularly, provide a kind of method of repairing pollution by manganese soil.
Background technology
Content of beary metal height, nutrition poorness, acid-base value is not suitable for retention ability a little less than etc. factor cause the manganese mine tailings to store up the region vegetation to lack, severe water and soil erosion, heavy metal spreads with rainwater and airborne dust.The manganese mine tailings have become a kind of manganese ore area important pollution sources.By screening and the suitable plant of cultivation, adopt simultaneously and make ancillary method, can in mine tailings are stored up district's short time, plant be settled down, thus revegetation.Some plant can absorb fixedly heavy metal at its root, and the plant that has can combine with heavy metal by the analyte of its root exudates and the thing that withers and falls thereof and form insoluble compound, thereby resistance control heavy-metal movement moves diffusion.The vegetation of rebuilding can be controlled the soil erosion and soil erosion, increases soil nutrient, recovers the function of degraded ecosystem and beautifies the environment.At large tracts of land mine tailings Polluted area revegetation, possess skills reliable, easy and simple to handle, cost is low, control multiple advantages such as pollutant is effective and environmentally friendly.
When the mine tailings laydown area carried out revegetation, the plant of selection should have that to absorb the pollutant ability strong or the root absorbability is strong, and growth is fast, well developed root system, and drought tolerance is strong and to features such as the heavy metal tolerance are strong; Select the plant of anti-soil depletion,, increase the nutrition of soil by the reparation of uniting of plant-microorganism.At present, there is not vegetation on the slag laydown area in the mine tailing storehouse of a lot of manganese ores, we find by experimental study, the acid-base value of the slag in the mine tailings laydown area is the critical limitation factor that plant survives in the above, because the alkalescence of slag and organic shortage, cause plant in mine tailing district soil, not grow, therefore when phytomicroorganism is united reparation, adopt the reinforcement regulation measure can promote that plant grows on mine tailing.
Based on our result of study, when phytoremediation manganese mine tailing storehouse, according to slag composition, nutrient content and acid-base value, the present invention chooses environment amenable plant, promote that by microbial-bacterial fertilizer plant grows on mine tailings, thereby unite a kind of new method that provides of repairing for fast and effeciently manganese tailings pollution ground being carried out phytomicroorganism.
Summary of the invention
The purpose of this invention is to provide a kind of method of repairing pollution by manganese soil, this method cost is low, improves mine entironment thereby the plant dyers' grapes are grown on the manganese mine tailings, and control is polluted, and can be widely used in the improvement reparation of mine pollution soil.
A kind of method of repairing pollution by manganese soil at alkaline manganese ore tailings laydown area plantation dyers' grapes, is characterized in that, during sowing, applies bacterial manure and mixed fertilizer earlier in the plantation hole, sows dyers' grapes seed, overburden soil on the seed after mixing again.
Described bacterial manure is the high anti-manganese bacterial strain that obtains by to screening from the castor-oil plant rhizosphere soil, carries out biofermentation and is made, and its preparation method step is as follows:
(1) get castor-oil plant rhizosphere soil sample, water and several beades, add in the bottle of having sterilized, placing rotating speed is that 150 r/min, temperature are that 27.5 ℃ shaking table vibrates, and gets suspension and does concentration gradient and be respectively 10
-2, 10
-3, 10
-4, 10
-5Bacteria suspension, the bacteria suspension of drawing variable concentrations with the spreading rod coating evenly, is inverted in 28 ℃ of incubators and is cultivated in corresponding Ma Dingshi culture medium; After 72h cultivates, observe microbial growth situation in the culture medium;
(2) adopt the plate streaking method of purification, choose single colony inoculation in Mn
2+Concentration is in the Ma Dingshi solid medium of 5500mg/L, is inverted in 28 ℃ of incubators and cultivates, and the purifying of ruling repeatedly is until obtaining pure bacterial strain;
(3) above-mentioned separation and purification being obtained the bacterial strain number consecutively is 1
#, 2
#, 3
#, being inoculated in the manganese concentration gradient in batches is in the liquid culture medium of 11000mg/L, 16500 mg/L, 22000 mg/L, 27500 mg/L, 33000mg/L, placing temperature is that 28 ℃, rotating speed are that the shaking table of 150 r/min is cultivated, and filters out 4
#Bacterium is the bacterial strain of high anti-manganese;
(4) the PDA culture medium is sub-packed in the test tube of sterilization, sterilization back pendulum inclined-plane, treat that the inclined-plane solidifies after, sterile working inoculation 4
#Bacterial classification is cultivated in 27.5 ℃ of incubators, and the spore growth of lawn surface is plentiful, is celadon, and dewdrop is arranged, and can take out standby;
(5) adopt starch, glucose, groundnut meal, peptone, ammonium sulfate, magnesium sulfate, dipotassium hydrogen phosphate and water preparation culture medium, with 10% NaOH adjust pH 7.2-7.4, with three conical flask packing, autoclaving, taking-up cools to till 40 ℃ of non-scald on hand, connects 4
#Bacterial classification shaken cultivation to the shaking table forms until mycelia, obtains mycelia;
(6) adopt groundnut meal, sucrose, ammonium sulfate, sweet potato flour, corn flour, peptone, calcium carbonate preparation culture medium, with fermentation tank disinfection and sterilization, then culture medium is put into fermentation tank, insert mycelia, continuous stirring, keep 28 ℃ of temperature, the throughput volume ratio is 1: 0.5, guarantees that each valve of fermentation tank is tight; Cultivate mycelia to ripe, mycelium is sturdy, and bacterium liquid is sticky foam.
(7) paddy skin, sweet potato flour, wheat bran, loam, ashes and soybean cake powder are sterilized, adopt two or more raw material preparation solid absorbent wherein then, put into the culturing room that adopts formaldehyde and sulfur fumigation to sterilize, inoculate mycelia then, cultivated 4-5 days, 35-45 ℃ of oven dry gets bacterium powder, i.e. bacterial manure.
Castor-oil plant rhizosphere soil sample in the described step (1) is the soil sample of 2mm around the root; PDA culture medium in the described step (4) adopts 200 parts of potatos, 20 parts of sucrose, and 20 parts in agar and 1000 parts in water are formulated; Culture medium in the described step 2 (2) adopts 2 parts of starch, 1 part of glucose, 2 parts of groundnut meals, 0.4 part of peptone, 0.25 part in ammonium sulfate, 0.025 part in magnesium sulfate, 0.02 part of dipotassium hydrogen phosphate and 1000 parts in water formulated; Culture medium in the described step (4) adopts 3 parts of groundnut meals, sucrose part, 0.25 part in ammonium sulfate, 2 parts of sweet potato flours, 1 part of corn flour, 0.1 part of peptone and 0.5 part in calcium carbonate formulated.
As preferred version one, solid absorbent in the described step (7) adopts 90 parts of paddy skins, 10 parts of sweet potato flours formulated, and the weight of described inoculation mycelia is 100% of solid absorbent.
As preferred version two, the solid absorbent in the described step (7) adopts 10 parts in wheat bran, 30 parts in ashes and 60 parts of loam formulated, and the weight of described inoculation mycelia is 40% of solid absorbent.
As preferred version three, the solid absorbent in the described step (7) adopts 10 parts of soybean cake powders, 60 parts of loam, 20 parts in ashes and 10 parts in wheat bran formulated, and the weight of described inoculation mycelia is 40% of solid absorbent.
Described paddy skin, sweet potato flour, soybean cake powder, wheat bran are through 121 ℃ of steam sterilizing 1h; Described loam, ashes are 160 ℃ of sterilization 1h on baker.
Described mixed fertilizer adopts 15 parts of rape cakes, 20 parts of animal wastes, 40 parts of paddy soils, 5 parts in urea, KH
2PO
410 parts, (NH
4)
2SO
412 parts, Ca (H
2PO
4)
215 parts formulated with 10 parts of CaSO4.
Beneficial effect of the present invention: dyers' grapes are perennial plants, and modes of reproduction is seminal propagation, and its alkali resistance and drought tolerance are strong, are dominant plants in the manganese ore district, and root is distributed in the dark soil of 0-70cm; Because it is acid that described bacterial manure and mixed fertilizer are, can weaken the alkalescence of alkaline mine tailings, can increase the water-soluble of mineral nutrition in the alkaline mine tailings, help the plant absorbing nutrient, and, grow normally so can make in the alkaline mine tailings of dyers' grapes a little less than retention ability, and can obtain a large amount of plant at short notice for fertile longer duration, behind first time artificial cultivation, do not need to sow once more.Elements such as a large amount of enrichment manganeses of dyers' grapes root energy, manganese element fixed efficiency height.The present invention can be used for mine degraded ecosystem phytomicroorganism and unites and repair and aspect such as saline land greening, and has the good and advantage such as beautify the environment of diffuser efficiency simple to operate, that expense is low, poisonous metal in the manganese tailings is controlled in resistance.
Below in conjunction with embodiment the present invention is further elaborated.
The specific embodiment
Embodiment 1:
(1) get castor-oil plant rhizosphere soil sample 10g and be added in the 250ml of the sterilization triangular flask that contains 90ml water and several beades, place shaking table (being set at 150 r/min, 27.5 ℃ of temperature) vibration 20min, getting suspension, to do concentration gradient respectively be 10
-2, 10
-3, 10
-4, 10
-5, (draw suspension 1ml in containing 9ml water test tube (sterilizing), this moment, bacteria suspension concentration was 10
-1, from the multiple above-mentioned steps of this concentration bacterium suspending weight, getting concentration is 10
-2Bacteria suspension, the rest may be inferred, concentration be respectively 10
-3, 10
-4, 10
-5Bacteria suspension); The bacteria suspension of drawing 0.1 ml variable concentrations separately with the spreading rod coating evenly, is inverted in 28 ℃ of incubators and is cultivated in corresponding Ma Dingshi culture medium; After 72h cultivates, observe microbial growth situation in the plating medium.
(2) adopt the plate streaking method of purification, choose single colony inoculation in Mn
2+Concentration is in the Ma Dingshi solid medium of 5500mg/L, is inverted in 28 ℃ of incubators and cultivates, and the purifying of ruling repeatedly is until obtaining pure bacterial strain.
(3) above-mentioned separation and purification being obtained the bacterial strain number consecutively is 1
#, 2
#, 3
#, be inoculated in the manganese concentration gradient in batches and be in the liquid culture medium of 11000mg/L, 16500 mg/L, 22000 mg/L, 27500 mg/L, 33000mg/L (28 ℃, 150 r/min clock shaking tables were cultivated 3 days), as a result 4
#Bacterial strain can be in 27500 mg/L culture mediums raised growth, and trace growth in the 33000mg/L culture medium, other bacterial strain all can not be grown in 27500 mg/L culture mediums.Filter out 4 thus
#Bacterial classification is the bacterial strain of high anti-manganese.
(4) adopt potato 200g, sucrose 20g, agar 20g and water 1000ml preparation PDA culture medium, pH nature; Compound method: potato cutting, poach filters, and filtrate is boiled dissolving agar with other compositions, is settled to 1000ml and gets final product.Get PDA culture medium 100ml, be sub-packed in the test tube of 170 ℃ of sterilizations of baking oven, tampon bandages with two-layer brown paper above beyond the Great Wall, 121 ℃ of sterilization 20min.Take out pendulum inclined-plane, back, treat that the inclined-plane solidifies after, sterile working inoculation 4# bacterial classification was cultivated 4 days in 27.5 ℃ of incubators, the spore growth of lawn surface is plentiful, is celadon, and dewdrop is arranged, and can take out standby.
(5) adopt starch 2g, glucose 1g, groundnut meal 20g, peptone 4g, ammonium sulfate 2.5g, magnesium sulfate 2.5g, dipotassium hydrogen phosphate 2g and water 1000ml preparation culture medium, use 10% NaOH adjust pH 7.2-7.4 then, with the packing of 1000ml conical flask, every bottle of 300ml, through 121 ℃ of autoclaving 20min, taking-up cools to till 40 ℃ of non-scald on hand, connects 4
#Bacterial classification shaken cultivation to the shaking table.The incipient stage that from the spore germination to the mycelia, forms, the about 24-36h of cell age.In this growth course, be subjected to about the influence institute of nutrition, pH value.Insert in seeding tank, the fermentation tank in this stage, under normal circumstances thalli growth is good.Put a jar back and observe, bacterium liquid is sticky, and micro-foam is arranged, and is blush, and borneol fragrance is arranged.
(6) adopt groundnut meal 3g, sucrose 1g, ammonium sulfate 0.25g, sweet potato flour 2g, corn flour 1g, peptone 0.1g and calcium carbonate 0.5g preparation culture medium, with fermentation tank disinfection and sterilization, 28 ℃ of jar temperature, 1: 0.5 (volume ratio) continuous stirring of throughput, often notice whether each valve is tight, otherwise make steam water enter the undergrowth that causes bacterium in the jar.Put a jar index, microscopy mycelia amount is many, and mycelium is sturdy, and mycelial growth is to the stage of ripeness, and bacterium liquid is sticky to have foam to become redness, generally cultivates 48h and can put jar.
(7) adopt paddy skin 90g, sweet potato flour 10g prepares solid absorbent.During preparation, paddy skin, sweet potato flour are being taken out behind 160 ℃ of sterilization 1h on the baker, be placed on (through formaldehyde and sulfur fumigation sterilization) in the culturing room, treating that material cools to inoculation about 40 ℃.Bacterium liquid and solid absorbent are in the inoculation of 1:1 ratio, and after the cultivation of ventilation pond was put in inoculation, bacteria containing amount can reach 130-200 * 10
8Individual/g.
(8) cultivate 4-5 days, with the bacterium powder drying pool 20-40cm that packs into, stir in the drying course for several times, drying time 12-24h, bake out temperature promptly get bacterial manure at 35-45 ℃, and be standby;
(9) adopt rape cake 15kg, animal wastes 20kg, paddy soils 40kg, urea 5kg, KH
2PO
410kg, (NH
4)
2SO
412kg, Ca (H
2PO
4)
215kg and CaSO4 10kg preparation mixed fertilizer, standby;
(10) manganese ore tailings natural air drying ground 0.5 mm aperture sieve, got pure manganese ore tailings, and was standby.Pure manganese mine tailings, bacterial manure and mixed fertilizer are mixed by mass ratio at 3: 1: 1, must improve the manganese ore tailings.Select 6 flowerpots, 3 dress improvement manganese ore tailings are adorned pure manganese mine tailings for 3, and loadings is the 3kg/ basin, install the every basin in the back 600ml that waters, and are placed with watertight plate below the flowerpot, place natural environment, in contrast.
(11) early March is broadcast the seed of dyers' grapes (Droopraceme Pokeweed) in 6 flowerpots, after planting waters 3 times.Statistics dyers' grapes seed germination rate after 10 days is lower than improvement manganese ore tailings though find pure manganese ore tailings germination percentage, and the two does not have significant difference.
(12) add up the survival rate of dyers' grapes seedling April, find that the average survival of improvement manganese mine tailings is 90.0%, and pure manganese mine tailings survival rate of plant only is 10%.
(13) September, measure soil pH value, organic content sees Table 1, and the organic matter in the improvement manganese mine tailings is apparently higher than control group, and the pH value descends, and is more conducive to the existence of plant.Dyers' grapes on the improvement manganese mine tailings grow fine, and can produce a large amount of seeds.
Organic matter in table 1 soil and pH value
| ? | Organic (g/kg) | pH |
| Contrast | 1.83±0.09 | 8.40±0.22 |
| Improvement manganese mine tailings | 5.76±0.12 | 6.57±0.34 |
Experiment shows, utilizes method provided by the invention, and plant can be survived and well-grown on the manganese ore tailings, and can natural propagation, repairs pollution by manganese soil.
Embodiment 2
In the step (7) of embodiment 1, adopt wheat bran 10g, ashes 30g, loam 60g prepares solid absorbent.During preparation, wheat bran is through 121 ℃ of steam sterilizing 1h, and loam, ashes are taking out behind 160 ℃ of sterilization 1h on the baker, is placed on (through formaldehyde and sulfur fumigation sterilization) in the culturing room, treats that material cools to inoculation about 40 ℃.Bacterium liquid and solid absorbent are inoculated in the 2:5 ratio.Other is with embodiment 1
Embodiment 3
In step (7) at embodiment 1, adopt soybean cake powder 10g, loam 60g, ashes 20g, wheat bran 10g prepares solid absorbent, during preparation, soybean cake powder, wheat bran are through 121 ℃ of steam sterilizing 1h, loam, ashes are taking out behind 160 ℃ of sterilization 1h on the baker, are placed on (through formaldehyde and sulfur fumigation sterilization) in the culturing room, treat that material cools to inoculation about 40 ℃.Bacterium liquid and solid absorbent are inoculated in the 2:5 ratio.Other is with embodiment 1.
Embodiment 4
(1) prepare bacterial manure by embodiment 1 or embodiment 2 or embodiment 3 described preparation methods, standby.
(2) adopt rape cake 150kg, animal wastes 200kg, paddy soils 400kg, urea 50kg, KH
2PO
4100kg, (NH
4)
2SO
4120kg, Ca (H
2PO
4)
2150kg and CaSO
4100kg prepares mixed fertilizer, and is standby;
(3) March, on the smooth sample of alkaline manganese ore tailings laydown area ground, dig the plantation hole, the specification in plantation hole is: 40-60 cm, wide 30-50 cm, dark 20-30 cm;
(4) apply bacterial manure 5.0kg-8.0kg in each plantation hole, mixed fertilizer A 4.0-5.0kg, and the two mixing.
(5) late March is sowed dyers' grapes (Droopraceme Pokeweed) seed in each plantation hole, and seed is broadcast above the mixed fertilizer behind mixing, and the about 2.5-3.5cm of overburden soil waters 2-3 time.
(6) April, add up the seedling number of seminal propagation in each ditch, found that seedling number of each average breeding in plantation hole is 32 strains, the dyers' grapes plant can be settled down and can raise up seed fast at manganese ore tailings laydown area.
(7) 8-9 month is gathered in the aerial part of dyers' grapes, and the plant of gathering in is covered the plant base portion.
Practice result shows, utilizes method provided by the invention, the plant dyers' grapes are survived on the manganese ore tailings and grow, and can natural propagation, improve pollution by manganese soil effectively.
Claims (10)
1. a method of repairing pollution by manganese soil at alkaline manganese ore tailings laydown area plantation dyers' grapes, is characterized in that, during sowing, applies bacterial manure and mixed fertilizer earlier in the plantation hole, sows the dyers' grapes seed after mixing again, and earthing waters.
2. the method for reparation pollution by manganese soil according to claim 1 is characterized in that, described bacterial manure is the high anti-manganese bacterial strain that obtains by to screening from the castor-oil plant rhizosphere soil, carries out biofermentation and is made, and its preparation method step is as follows:
(1) get castor-oil plant rhizosphere soil sample, water and several beades, add in the bottle of having sterilized, placing rotating speed is that 150 r/min, temperature are that 27.5 ℃ shaking table vibrates, and gets suspension and does concentration gradient and be respectively 10
-2, 10
-3, 10
-4, 10
-5Bacteria suspension, the bacteria suspension of drawing variable concentrations with the spreading rod coating evenly, is inverted in 28 ℃ of incubators and is cultivated in corresponding Ma Dingshi culture medium; After 72h cultivates, observe microbial growth situation in the culture medium;
(2) adopt the plate streaking method of purification, choose single colony inoculation in Mn
2+Concentration is in the Ma Dingshi solid medium of 5500mg/L, is inverted in 28 ℃ of incubators and cultivates, and the purifying of ruling repeatedly is until obtaining pure bacterial strain;
(3) above-mentioned separation and purification being obtained the bacterial strain number consecutively is 1
#, 2
#, 3
#, being inoculated in the manganese concentration gradient in batches is in the liquid culture medium of 11000mg/L, 16500 mg/L, 22000 mg/L, 27500 mg/L, 33000mg/L, placing temperature is that 28 ℃, rotating speed are that the shaking table of 150 r/min is cultivated, and filters out 4
#Bacterium is the bacterial strain of high anti-manganese;
(4) the PDA culture medium is sub-packed in the test tube of sterilization, sterilization back pendulum inclined-plane, treat that the inclined-plane solidifies after, sterile working inoculation 4
#Bacterial classification is cultivated in 27.5 ℃ of incubators, and the spore growth of lawn surface is plentiful, is celadon, and dewdrop is arranged, and can take out standby;
(5) adopt starch, glucose, groundnut meal, peptone, ammonium sulfate, magnesium sulfate, dipotassium hydrogen phosphate and water preparation culture medium, with 10% NaOH adjust pH 7.2-7.4, with three conical flask packing, autoclaving, taking-up cools to till 40 ℃ of non-scald on hand, connects 4
#Bacterial classification shaken cultivation to the shaking table forms until mycelia, obtains mycelia;
(6) adopt groundnut meal, sucrose, ammonium sulfate, sweet potato flour, corn flour, peptone, calcium carbonate preparation culture medium, with fermentation tank disinfection and sterilization, then culture medium is put into fermentation tank, insert mycelia, continuous stirring, keep 28 ℃ of temperature, the throughput volume ratio is 1: 0.5, guarantees that each valve of fermentation tank is tight; Cultivate mycelia to ripe, mycelium is sturdy, and bacterium liquid is sticky foam;
(7) paddy skin, sweet potato flour, wheat bran, loam, ashes and soybean cake powder are sterilized, adopt two or more raw material preparation solid absorbent wherein then, put into the culturing room that adopts formaldehyde and sulfur fumigation to sterilize, inoculate mycelia then, cultivated 4-5 days, 35-45 ℃ of oven dry gets bacterium powder, i.e. bacterial manure.
3. the method for reparation pollution by manganese soil according to claim 2 is characterized in that: the castor-oil plant rhizosphere soil sample in the described step (1) is the soil sample of 2mm around the root; PDA culture medium in the described step (4) adopts 200 parts of potatos, 20 parts of sucrose, and 20 parts in agar and 1000 parts in water are formulated; Culture medium in the described step 2 (2) adopts 2 parts of starch, 1 part of glucose, 2 parts of groundnut meals, 0.4 part of peptone, 0.25 part in ammonium sulfate, 0.025 part in magnesium sulfate, 0.02 part of dipotassium hydrogen phosphate and 1000 parts in water formulated; Culture medium in the described step (4) adopts 3 parts of groundnut meals, sucrose part, 0.25 part in ammonium sulfate, 2 parts of sweet potato flours, 1 part of corn flour, 0.1 part of peptone and 0.5 part in calcium carbonate formulated.
4. the method for reparation pollution by manganese soil according to claim 2 is characterized in that: solid absorbent in the described step (7) adopts 90 parts of paddy skins, 10 parts of sweet potato flours formulated.
5. the method for reparation pollution by manganese soil according to claim 2 is characterized in that: the solid absorbent in the described step (7) adopts 10 parts in wheat bran, 30 parts in ashes and 60 parts of loam formulated.
6. the method for reparation pollution by manganese soil according to claim 2 is characterized in that: the solid absorbent in the described step (7) adopts 10 parts of soybean cake powders, 60 parts of loam, 20 parts in ashes and 10 parts in wheat bran formulated.
7. according to the method for claim 4 or 5 or 6 described reparation pollution by manganese soil, it is characterized in that: described paddy skin, sweet potato flour, soybean cake powder, wheat bran are through 121 ℃ of steam sterilizing 1h; Described loam, ashes are 160 ℃ of sterilization 1h on baker.
8. the method for reparation pollution by manganese soil according to claim 4 is characterized in that: the weight of described inoculation mycelia is 100% of solid absorbent.
9. according to the method for claim 5 or 6 described reparation pollution by manganese soil, it is characterized in that: the weight of described inoculation mycelia is 40% of solid absorbent.
10. the method for reparation pollution by manganese soil according to claim 1 is characterized in that, described mixed fertilizer adopts 15 parts of rape cakes, 20 parts of animal wastes, 40 parts of paddy soils, 5 parts in urea, KH
2PO
410 parts, (NH
4)
2SO
412 parts, Ca (H
2PO
4)
215 parts formulated with 10 parts of CaSO4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101212988A CN102240664A (en) | 2011-05-11 | 2011-05-11 | Method for restoring soil polluted by manganese |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101212988A CN102240664A (en) | 2011-05-11 | 2011-05-11 | Method for restoring soil polluted by manganese |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102240664A true CN102240664A (en) | 2011-11-16 |
Family
ID=44959016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101212988A Pending CN102240664A (en) | 2011-05-11 | 2011-05-11 | Method for restoring soil polluted by manganese |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102240664A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102632074A (en) * | 2012-04-19 | 2012-08-15 | 西南大学 | Method for repairing heavy metal polluted soil by using radix achyranthis bidentatae as manganese hyperaccumulator |
| CN102676807A (en) * | 2012-05-18 | 2012-09-19 | 中南大学 | Method for leaching manganese oxide |
| CN103922822A (en) * | 2014-03-03 | 2014-07-16 | 江苏上田环境修复有限公司 | Method for making manganese-containing organic fertilizer by utilization of manganese hyperaccumulation remediation plant Phytolacca acinosa |
| CN103962369A (en) * | 2014-04-30 | 2014-08-06 | 中南林业科技大学 | Method for remedying heavy metal contaminated soil by energy plant configuration mode |
| CN104096710A (en) * | 2014-07-02 | 2014-10-15 | 西南科技大学 | Method for removing heavy metal in soil in situ by cultivating mycoderma of helicobasidium mompa |
| CN105993260A (en) * | 2016-05-13 | 2016-10-12 | 中南林业科技大学 | Method for restoring degraded wetland vegetation by improved wetland soil seed banks |
| CN106269848A (en) * | 2016-08-24 | 2017-01-04 | 宁波枫叶杰科生物技术有限公司 | A kind of method extracting heavy metal resistance strain secretes polypeptide improvement heavy-metal contaminated soil |
| CN107081334A (en) * | 2017-06-12 | 2017-08-22 | 湖南科技大学 | The method of safe Planting Crops on soil polluted by manganese |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
| CN107980266A (en) * | 2017-12-09 | 2018-05-04 | 湖南科技大学 | Suppress the method for brassica plant absorption cadmium using the bacterium for producing ABA |
| CN108668552A (en) * | 2018-04-16 | 2018-10-19 | 中山大学 | A kind of ion type rareearth tailings ground modifying agent and its restorative procedure |
| CN112264460A (en) * | 2020-09-30 | 2021-01-26 | 青岛衡立环境技术研究院有限公司 | Method for enhancing capability of pokeberry to restore petroleum-polluted soil |
-
2011
- 2011-05-11 CN CN2011101212988A patent/CN102240664A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102632074A (en) * | 2012-04-19 | 2012-08-15 | 西南大学 | Method for repairing heavy metal polluted soil by using radix achyranthis bidentatae as manganese hyperaccumulator |
| CN102676807A (en) * | 2012-05-18 | 2012-09-19 | 中南大学 | Method for leaching manganese oxide |
| CN103922822A (en) * | 2014-03-03 | 2014-07-16 | 江苏上田环境修复有限公司 | Method for making manganese-containing organic fertilizer by utilization of manganese hyperaccumulation remediation plant Phytolacca acinosa |
| CN103922822B (en) * | 2014-03-03 | 2016-04-20 | 上田环境修复股份有限公司 | A kind of manganese ultraproduct that utilizes tires out the method for rehabilitation plant Phytolacca acinosa making containing manganese organic fertilizer |
| CN103962369A (en) * | 2014-04-30 | 2014-08-06 | 中南林业科技大学 | Method for remedying heavy metal contaminated soil by energy plant configuration mode |
| CN103962369B (en) * | 2014-04-30 | 2016-01-20 | 中南林业科技大学 | A kind of method utilizing energy-source plant configuration mode restoration of soil polluted by heavy metal |
| CN104096710B (en) * | 2014-07-02 | 2016-03-30 | 西南科技大学 | A kind of method of cultivating purple striae plumage volume load bacterium mycoderma original position removal heavy metal in soil |
| CN104096710A (en) * | 2014-07-02 | 2014-10-15 | 西南科技大学 | Method for removing heavy metal in soil in situ by cultivating mycoderma of helicobasidium mompa |
| CN105993260A (en) * | 2016-05-13 | 2016-10-12 | 中南林业科技大学 | Method for restoring degraded wetland vegetation by improved wetland soil seed banks |
| CN106269848A (en) * | 2016-08-24 | 2017-01-04 | 宁波枫叶杰科生物技术有限公司 | A kind of method extracting heavy metal resistance strain secretes polypeptide improvement heavy-metal contaminated soil |
| CN107081334A (en) * | 2017-06-12 | 2017-08-22 | 湖南科技大学 | The method of safe Planting Crops on soil polluted by manganese |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
| CN107980266A (en) * | 2017-12-09 | 2018-05-04 | 湖南科技大学 | Suppress the method for brassica plant absorption cadmium using the bacterium for producing ABA |
| CN108668552A (en) * | 2018-04-16 | 2018-10-19 | 中山大学 | A kind of ion type rareearth tailings ground modifying agent and its restorative procedure |
| CN112264460A (en) * | 2020-09-30 | 2021-01-26 | 青岛衡立环境技术研究院有限公司 | Method for enhancing capability of pokeberry to restore petroleum-polluted soil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102240664A (en) | Method for restoring soil polluted by manganese | |
| CN101054568B (en) | Special biological organic fertilizer for tobacco, preparation and use method | |
| CN103125251B (en) | Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation | |
| CN100579940C (en) | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof | |
| CN103740693B (en) | A kind of straw decomposing inoculant and preparation method thereof | |
| CN106701095B (en) | Soil conditioner and application thereof in desert control | |
| CN102030581B (en) | Novel multi-functional biological organic seed dressing agent and method for preparing same | |
| CN106591193A (en) | Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects | |
| CN103435380B (en) | Device and method for planting fast-growing plants and replacing chemical fertilizers with plant composts | |
| CN110106126A (en) | One plant of colloid bacillus cereus and its preparing the application in saline-alkali soil conditioner | |
| CN108191480A (en) | A kind of biological organic fertilizer and preparation method thereof | |
| CN110184220A (en) | One plant height imitates dissolving phosphor and dissolving potassium bacterium and its application | |
| CN112501072B (en) | Crop rhizosphere soil conditioner and preparation method and application thereof | |
| CN110100670A (en) | A method of prevention and treatment crop in cruciferae clubroot | |
| CN112538431A (en) | Chlorella pyrenoidosa and biological environment restoration liquid prepared from chlorella pyrenoidosa and application of chlorella pyrenoidosa | |
| CN104911129A (en) | Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof | |
| CN101886055B (en) | Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt | |
| CN110437000A (en) | A kind of microbial manure and its preparation method and application | |
| CN108409411B (en) | Biological seedling strengthening agent for enhancing stress resistance of rice and preparation method thereof | |
| CN108034620A (en) | One plant of bacillus megaterium bacterial strain, peanut drop cadmium agent and its application | |
| Sethi et al. | Microbial biofertilizers and their pilot-scale production | |
| CN107382496A (en) | A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity | |
| CN102603372B (en) | Simple production technology of special AM (arbuscular mycorrhiza) fungal manure for tobacco | |
| CN110218678A (en) | Anti- continuous cropping soil renovation agent and preparation method thereof | |
| CN112119864B (en) | Ceramsite soilless culture substrate as well as preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111116 |