CN110184220A - One plant height imitates dissolving phosphor and dissolving potassium bacterium and its application - Google Patents
One plant height imitates dissolving phosphor and dissolving potassium bacterium and its application Download PDFInfo
- Publication number
- CN110184220A CN110184220A CN201910475323.9A CN201910475323A CN110184220A CN 110184220 A CN110184220 A CN 110184220A CN 201910475323 A CN201910475323 A CN 201910475323A CN 110184220 A CN110184220 A CN 110184220A
- Authority
- CN
- China
- Prior art keywords
- potassium
- dissolving
- culture
- soil
- decomposing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011591 potassium Substances 0.000 title claims abstract description 127
- 229910052700 potassium Inorganic materials 0.000 title claims abstract description 127
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 title claims abstract description 126
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 99
- 241000894006 Bacteria Species 0.000 title claims abstract description 66
- 239000002689 soil Substances 0.000 claims abstract description 64
- 239000011574 phosphorus Substances 0.000 claims abstract description 56
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 56
- 230000001580 bacterial effect Effects 0.000 claims abstract description 27
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 11
- 230000008635 plant growth Effects 0.000 claims abstract description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 10
- 239000002609 medium Substances 0.000 claims description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 239000003337 fertilizer Substances 0.000 claims description 36
- 239000002068 microbial inoculum Substances 0.000 claims description 34
- 239000007787 solid Substances 0.000 claims description 34
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 28
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 24
- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 230000003381 solubilizing effect Effects 0.000 claims description 19
- 239000001963 growth medium Substances 0.000 claims description 17
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 16
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 14
- 229940095054 ammoniac Drugs 0.000 claims description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 12
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 239000001506 calcium phosphate Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000001103 potassium chloride Substances 0.000 claims description 8
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 6
- 239000010433 feldspar Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 238000012549 training Methods 0.000 claims description 5
- 229940078499 tricalcium phosphate Drugs 0.000 claims description 5
- 235000019731 tricalcium phosphate Nutrition 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 238000009331 sowing Methods 0.000 claims 1
- 235000013311 vegetables Nutrition 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000035558 fertility Effects 0.000 abstract description 2
- 150000003112 potassium compounds Chemical class 0.000 abstract description 2
- 239000002686 phosphate fertilizer Substances 0.000 abstract 1
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 35
- 241000196324 Embryophyta Species 0.000 description 31
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 29
- 239000000243 solution Substances 0.000 description 28
- 230000012010 growth Effects 0.000 description 19
- 235000015097 nutrients Nutrition 0.000 description 17
- 239000008223 sterile water Substances 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 12
- 239000012530 fluid Substances 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 239000003610 charcoal Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 108020004465 16S ribosomal RNA Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 229910019142 PO4 Inorganic materials 0.000 description 5
- 239000002585 base Substances 0.000 description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 description 5
- 235000010755 mineral Nutrition 0.000 description 5
- 239000011707 mineral Substances 0.000 description 5
- 235000021317 phosphate Nutrition 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 239000012533 medium component Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 238000004382 potting Methods 0.000 description 4
- 230000007226 seed germination Effects 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000021749 root development Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000004016 soil organic matter Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 108010011619 6-Phytase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- YSMRWXYRXBRSND-UHFFFAOYSA-N TOTP Chemical compound CC1=CC=CC=C1OP(=O)(OC=1C(=CC=CC=1)C)OC1=CC=CC=C1C YSMRWXYRXBRSND-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- DUHSSNQZRMKHLD-UHFFFAOYSA-N [Ca].[Ca].[Ca].P(O)(O)(O)=O Chemical compound [Ca].[Ca].[Ca].P(O)(O)(O)=O DUHSSNQZRMKHLD-UHFFFAOYSA-N 0.000 description 1
- ZGBSOTLWHZQNLH-UHFFFAOYSA-N [Mg].S(O)(O)(=O)=O Chemical compound [Mg].S(O)(O)(=O)=O ZGBSOTLWHZQNLH-UHFFFAOYSA-N 0.000 description 1
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008638 plant developmental process Effects 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- 150000003109 potassium Chemical class 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Pest Control & Pesticides (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Soil Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a plant heights to imitate dissolving phosphor and dissolving potassium bacterium, its classification naming is Bei Laisi bacillus (Bacillus velezensis), bacterial strain X-P18, it has been preserved in China typical culture collection center, deposit number is CCTCC NO:M 2019317, and the deposit date is on April 29th, 2019.Dissolving phosphor and dissolving potassium bacterium of the invention can convert the Phos of slightly solubility, potassium to high-quality phosphorus, the potassium compound being directly absorbed and utilized for plant, improve insoluble phosphorus, the biological effectiveness of potassium and phosphate fertilizer, potassium utilization rate in soil, with plant growth can be promoted, the advantages that without secondary pollution, applied widely, at low cost, and be of great significance and application value in terms of cultivating and playing soil ecology fertility, keep Agro-ecology balance.
Description
Technical field
The present invention relates to microorganisms technical fields, and in particular to a plant height imitates dissolving phosphor and dissolving potassium bacterium and its application.
Background technique
Phosphorus is one of essential element in plant growth and development process, almost participates in new old generation all in plant
Apologize for having done sth. wrong journey, such as photosynthesis, energy transmission, organic synthesis, signal transduction etc., at the same still cell amplifying nucleic acid, phosphorous enzyme,
The important component of the substances such as ATP, protein.ATP is plant energy i (in vivo) stored substance and referred to as energy currency, albumen
Matter and nucleic acid be form life material base, the substances such as enzyme participate in plant in catalysis reaction guarantee metabolism smoothly into
Row.P elements also have facilitation to the growth and development of the elongation of root system of plant, plant, improve plant to the resistance of environment.
The available P that plant can be absorbed and utilized in soil only accounts for the 2%~3% of full phosphorus amount, and most phosphorus cannot directly be planted
Object absorbs, and needs just be eventually converted into the substance that can be utilized by plant by a series of physics, chemistry and biological respinse.Soil
Phosphorus is non-renewable mineral resource in earth, and there is 1.3 hundred million hectares of farmland in China, and phosphorus content is very high in 74% arable land, but has
The content of phosphorus is imitated less than 5%, and remaining arable land then seriously lacks phosphorus.
The rich content of potassium in the earth's crust, average out to 2.6%, and can reach 3% in mineral soil, the content of potassium is in the earth's crust
The 4th is come in contained all nutrients, and the content of potassium is highest in the nutrient contained by soil.Soil
The form of diverse of potassium in earth, can be divided into mineral K, water-soluble potassium, noncommutative wavelet and exchangeable potassium.Full potassium content in soil
90% or more is to exist in the form of mineral K, and this potassium is only released from mineral and can be just absorbed and used by plants.
In the soil, by that can change in each form with some physics, chemistry or biological activity potassium and there is a kind of dynamic is flat
Weighing apparatus, therefore the existence form of the potassium in soil can be adjusted by way of artificial interference.Under normal circumstances, plant needs potassium amount
It is suitable with nitrogen, or even demand will also be more than nitrogen under special circumstances.However, in some areas, in soil, potassium content deficiency has been
An important factor for as crop yield is restricted.
Phosphate solubilizing microorganism has huge conversion capability to difficultly-soluble phosphates in soil, and can secrete needed for plant growth
Auximone substance promotes plant growth and development.The acidic materials that phosphate solubilizing bacteria generates can accelerate phosphorous insoluble organise
The decomposition of object is closed, phytase, nuclease and the phosphatase etc. of secretion can be chelated with Phos, be made not available inorganic
Phosphorus is converted into available phosphorus, promotes phosphorus element release；It is extracellular that potassium solubilizing bacteria generates a large amount of high-viscosity material one during growth and breeding
Polysaccharide and capsular polysaccharide.These glucides not only have multi-functional physiological activity, but also form soil aggregate structure
Adhesive, generally forms that small crumb structure body is more around root system, is exactly the cementing grogs of polysaccharide of cell secretion as a result, group
The formation of kernel structure make soil become dredging, it is soft, the enhancing of retain water and nutrients performance, water, gas heat are more coordinated.If using dissolving phosphor and dissolving potassium
For bacterium as bio-feritlizer, advantage is obvious: at low cost, effect is good, and sustained release is sustainable, can not only increase after application
Crop yield improves soil quality and structure, moreover it is possible to improve soil organic matter content, improve the effective use of phosphorus, potassium in soil
Rate saves fertilizer volume increase, is of great significance to keeping ecological environment to balance.
Summary of the invention
Technical problem to be solved by the present invention lies in the screenings for passing through dissolving phosphor and dissolving potassium solid medium, and providing one kind has
Degradation soil indissoluble Phos, potassium, a kind of efficient phosphate-solubilizing potassium decomposing of the high-quality phosphorus, potassium that can directly absorb is provided for plant
Bacterium.
The present invention also technical problems to be solved are to provide the application of above-mentioned dissolving phosphor and dissolving potassium bacterial strain.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
One plant height imitates dissolving phosphor and dissolving potassium bacterium, and classification naming is Bei Laisi bacillus (Bacillus velezensis), bacterium
Strain X-P18 has been preserved in China typical culture collection center (abbreviation CCTCC), and address is the Wuhan Wuhan University, China,
Postcode is 430072, and deposit number is CCTCC NO:M 2019317, and the deposit date is on April 29th, 2019.The bacterial strain is hair
Bright people is in the bacterial strain of on the May 15th, 2018 of breeding in the soil sample of farmland area near Ma'an Mountain, Anhui Province potassium mine.
The process of screening, includes the following steps:
A. the preparation of Selective agar medium
B. the primary dcreening operation of dissolving phosphor and dissolving potassium bacterium
C. the secondary screening of dissolving phosphor and dissolving potassium bacterium
D. dissolving phosphor and dissolving potassium bacterial strain ?application in leaf palm-leaf fun Chinese cabbage potting
The step a may further include: preparation phosphorus decomposing solid medium
The step b may further include: 5g soil being taken to be added to enrichment culture in the sterile water of 50ml, condition of culture 20
DEG C, 160r/min cultivate 2h；Take enrichment culture liquid by 10-1、10-2、10-3、10-4、10-5、10-6Gradient dilution is trained in phosphorus decomposing solid
It supports and is coated on base, purifying scribing line culture 5-6 times when growing single colonie is trained by Phos fluid nutrient medium, inorganic potassium liquid
The secondary screening of base is supported, to obtain efficient dissolving phosphor and dissolving potassium bacterial strain.
The step c may further include: by the strain inoculated screened in 100ml Phos fluid nutrient medium and nothing
In machine potassium fluid nutrient medium, 30 DEG C of 160r/min cultivate 4d and 7d respectively, measure the Soluble phosphorus ability of dissolving potassium of bacterial strain.
The step d may further include: the efficient phosphate-solubilizing potassium decomposing bacterial strain that screening obtains being configured to microbial inoculum and is applied to
?in leaf palm-leaf fun Chinese cabbage potting.
Bacterial strain identification method:
16sRNA sequence analysis: PCR amplification uses bacterium 16sRNA universal primer:
27F(5’-AGAGTTTGATCMTGGCTCAG-3’)
1492R(5’-GGTTACCTTGTTACGACTT-3’)
PCR reaction system (2.5 μ L): 5 × amplification buffer (5 μ L), genomic DNA (0.1 μ L),
DNTP (2 μ L), primers F (0.5 μ L), primer R (0.5 μ L), Taq archaeal dna polymerase (0.25 μ L), deionized water
(16.65μL).Reaction condition: 98 DEG C of initial denaturation 10min, 98 DEG C of deformation 10s, 58 DEG C of annealing 10s, 72 DEG C of extension 90s, totally 30
Circulation, 72 DEG C sufficiently extend 10min.
16S rDNA major part sequence is measured, as shown in SEQ ID No:1.
Strain X-P18 has the following properties:
1, colonial morphology feature:
On peptone agar medium 30 DEG C culture 1~2 day after microscope observe vegetative cell be unicellular, bar
Shape has oval gemma.30 DEG C of culture 12h thallus can be with raised growth in above-mentioned culture medium.Bacterium colony is creamy white, round,
Surface is smooth, neat in edge, sticky, intermediate projections.
2, physio-biochemical characteristics:
(1) cultivation temperature: 20-50 DEG C.
(2) it is grown in the range of pH4.5~11；
(3) it is grown in 1%~8% range of salinity
(4) Gram's staining: positive
(5) catalase: positive
(6) V-P reacts: positive
(7) Starch Hydrolysis enzyme reaction: positive
(8) anaerobic condition: positive
(9) citrate utilizes: positive
(10) sugar alcohol ferments: using mannitol, xylose, cellobiose, maltose, lactose
(11) M-R reacts: positive
3,16S rDNA sequence is analyzed
16sRNA sequence analysis: PCR amplification uses bacterium 16sRNA universal primer:
27F(5’-AGAGTTTGATCMTGGCTCAG-3’)
1492R(5’-GGTTACCTTGTTACGACTT-3’)
PCR reaction system (2.5 μ L): 5 × amplification buffer (5 μ L), genomic DNA (0.1 μ L),
DNTP (2 μ L), primers F (0.5 μ L), primer R (0.5 μ L), Taq archaeal dna polymerase (0.25 μ L), deionized water
(16.65μL).Reaction condition: 98 DEG C of initial denaturation 10min, 98 DEG C of deformation 10s, 58 DEG C of annealing 10s, 72 DEG C of extension 90s, totally 30
Circulation, 72 DEG C sufficiently extend 10min.
16S rDNA major part sequence is measured, as shown in SEQ ID No:1.The BLAST of the column website NCBI will be sequenced
The comparison of base sequence is carried out, the phylogenetic tree based on 16S rDNA complete sequence is constructed.The result shows that: bacterial strain and Bei Laisi
It is homologous that bacillus reaches 98%.So assert that the present invention uses Bei Laisi bacillus (Bacillus
Velezensis), specific location Bei Laisi bacillus velezensis X-P18.
Above-mentioned efficient phosphate-solubilizing potassium solubilizing bacteria in soil phosphorus decomposing and/or potassium decomposing application also protection scope of the present invention it
It is interior.
Wherein, efficient phosphate-solubilizing potassium solubilizing bacteria is prepared as follows into the phosphorus decomposing and/or potassium decomposing that microbial inoculum is used for soil, applied
Amount is 1.3-2.0L/m2:
It will be stored in -20 DEG C of dissolving phosphor and dissolving potassium bacterium activation, is coated on phosphorus decomposing solid medium, culture 16~for 24 hours；Picking solution
Strain on phosphorus solid medium is purified on fresh phosphorus decomposing solid medium and is crossed, and culture 16~for 24 hours；Picking phosphorus decomposing is solid
Single colonie on body culture medium, is inoculated in fermentation medium, and 30 DEG C of cultures 16~for 24 hours, expand training in fermentation medium
It supports, is 10 to bacterial concentration8-109When CFU/g, phosphorus decomposing of the fermentation liquid for soil is obtained, alternatively,
It will be stored in -20 DEG C of dissolving phosphor and dissolving potassium bacterium activation, is coated on potassium decomposing solid medium, culture 16~for 24 hours；Picking solution
Strain on potassium solid medium is purified on fresh potassium decomposing solid medium and is crossed, and culture 16~for 24 hours；Picking potassium decomposing is solid
Single colonie on body culture medium, is inoculated in fermentation medium, and 30 DEG C of cultures 16~for 24 hours, expand training in fermentation medium
It supports, is 10 to bacterial concentration8-109When CFU/g, potassium decomposing of the fermentation liquid for soil is obtained.
Wherein, the phosphorus decomposing solid culture based formulas are as follows: glucose 5g, tricalcium phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac
0.5g, potassium chloride 1.7g, calcium carbonate 0.1g, ferric sesquichloride 0.005g, agar 20g, water 1000ml.
Fermentation medium are as follows: glucose 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, calcium carbonate 0.1g,
Ferric sesquichloride 0.005g, water 1000ml.
Potassium decomposing solid culture based formulas are as follows: sucrose 5g, feldspar in powder 3g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, biphosphate
Sodium 3.14g, calcium carbonate 0.1g, ferric sesquichloride 0.005g, agar 20g, water 1000ml.
Application of the above-mentioned efficient phosphate-solubilizing potassium solubilizing bacteria in promotion plant growth, reduction fertilizer amount is also in protection of the invention
Within the scope of.
Above-mentioned efficient phosphate-solubilizing potassium solubilizing bacteria can be used in mixed way with charcoal, also can be used alone, and improve rhizosphere microorganism ring
Border promotes plant growth, reduces fertilizer application amount.
Efficient phosphate-solubilizing potassium solubilizing bacteria of the invention is at 30 DEG C, shaken cultivation 4 days under the conditions of 160rpm, can be by 5.0g/L tricresyl phosphate
Calcium phosphorus decomposing, water-soluble phosphorus content are 416.62-534.52mg/L；At 30 DEG C, shaken cultivation 7 days under the conditions of 160rpm, by 3.0g/
L feldspar in powder potassium decomposing, water-soluble potassium content are 2.78-3.48mg/L.
Efficient phosphate-solubilizing potassium decomposing bacterial strain of the invention can survive in the LB culture medium that pH range is 4.5-11, and highest can also
It survives in the LB culture medium of salinity 8wt%, survives under the conditions of 20-50 DEG C of temperature, there is alkali resistance, the salt tolerance of height, energy
It is enough to be applied in salt-soda soil.
The utility model has the advantages that efficient phosphate-solubilizing potassium solubilizing bacteria of the invention and its microbial inoculum being prepared have the advantage that
The present invention filters out the dissolving phosphor and dissolving potassium bacterium Bacillus with degradation slightly solubility Phos, potassium from soil
Velezensis X-P18, and solution is prepared using phosphate solubilizing bacteria Bacillus velezensis X-P18 as strain fermentation culture
Phosphorus potassium decomposing microbial inoculum, the dissolving phosphor and dissolving potassium microbial inoculum can convert slightly solubility Phos, potassium to be directly absorbed and utilized for plant it is excellent
Insoluble phosphorus in soil, the biological effectiveness of potassium and phosphorus, potash fertilizer utilization efficiency can be improved in matter phosphorus, potassium compound, saves fertilizer and increases
It produces, and soil texture can be improved, improve soil organic matter content, to giving full play to soil fertility, keep agroecological environment
Balance etc. all have extremely important meaning and application value.
Dissolving phosphor and dissolving potassium bacterium provided by the invention and its microbial inoculum not only can increase available phosphorus, potassium in soil, but also can promote
Absorption of the plant to other nutrients, dissolving phosphor and dissolving potassium bacterium can adsorb the nutrients such as zinc, copper, calcium around root system of plant,
Improve plant nutrient；But also growth regulatory substance can be secreted, promote root growth；And it after the inoculation of dissolving phosphor and dissolving potassium bacterium, is planting
The breeding of object rhizosphere raised growth, becomes the dominant bacteria of plant rhizosphere, to can inhibit or reduce the micro- life of cause of disease whithin a period of time
The growth machine meeting of object, improves the disease resistance of plant.
Dissolving phosphor and dissolving potassium bacterium provided by the invention and can by slightly solubility Phos in soil, that potassium is degraded to plant is utilizable
Soluble Inorganic Phosphorus, potassium directly discharge high-quality phosphorus, potassic fertilizer into soil, therefore there is no dirty to environmental emission in production process
Object is contaminated, dissolving phosphor and dissolving potassium microorganism is a kind of environment-friendly type fertilizer truly；And dissolving phosphor and dissolving potassium bacterium provided by the invention and
Preparation method, simple production process, high production efficiency are able to achieve industrialized production, have good economic benefits and society
Effect.
Detailed description of the invention
Fig. 1 is X-P18 Gram's staining.
Fig. 2 is X-P18 colonial morphology on LB plate.
Fig. 3 is phosphorus decomposing effect of the X-P18 in phosphorus decomposing fluid nutrient medium.
Fig. 4 is potassium decomposing effect of the X-P18 in potassium decomposing fluid nutrient medium.
Fig. 5 is X-P18 systematic evolution tree
Specific embodiment
The present invention is furture elucidated With reference to embodiment, it should be understood that these embodiments are merely to illustrate the present invention
Rather than limit the scope of the invention, after the present invention has been read, those skilled in the art are to various equivalences of the invention
The modification of form falls within the application range as defined in the appended claims.
The screening of 1: Bei Laisi bacillus X-P18 of embodiment
(1) pedotheque source: nearby farmland is regional for Ma'an Mountain's potassium mine in Anhui city, Jiangsu Province
(2) bacterial strain screening: 5g soil is taken to be added to enrichment culture in the sterile water of 50ml, 20 DEG C of condition of culture, 160r/min
Cultivate 2h；Take enrichment culture liquid by 10-1、10-2、10-3、10-4、10-5、10-6Gradient dilution is coated on phosphorus decomposing solid medium,
Purifying scribing line culture 5-6 times when growing single colonie, by the secondary screening of Phos fluid nutrient medium, inorganic potassium fluid nutrient medium,
To obtain efficient dissolving phosphor and dissolving potassium bacterial strain.
Phos fluid nutrient medium, medium component are glucose 10g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, calcium carbonate
0.1g, ferric sesquichloride 0.005g, tricalcium phosphate 5g, water 1000ml.
Inorganic potassium fluid nutrient medium, medium component are sucrose 10g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, calcium carbonate
0.1g, sodium dihydrogen phosphate 3.14g, ferric sesquichloride 0.005g, feldspar in powder 3g, water 1000ml.
(3) colony morphological observation of X-P18 bacterial strain
By the X-P18 bacterial strain screened on LB solid medium 30 DEG C culture 1~2 day after carry out Gram's staining, lead to
Cross microscope observe vegetative cell be it is unicellular, it is rod-shaped, have oval gemma, as shown in Figure 1.30 DEG C in above-mentioned culture medium
Cultivating 12h thallus can be with raised growth.Bacterium colony is creamy white, and round, surface is smooth, neat in edge, sticky, intermediate projections, such as
Shown in Fig. 2.
Embodiment 2: the dissolving P capacity measurement of bacterial strain.
100ml Phos fluid nutrient medium is separately added into 250ml triangular flask, medium component is glucose 10g, sulphur
Sour magnesium 0.5g, sulfate of ammoniac 0.5g, calcium carbonate 0.1g, ferric sesquichloride 0.005g, tricalcium phosphate 5g, water 1000ml.Inoculate 2ml use
The bacteria suspension of sterile water preparation, while doing and not being inoculated with control (2ml sterile water is added in culture medium), all processing carry out 3 times
It repeats.At 30 DEG C, 160rpm vibrates 4d respectively, and culture solution is centrifuged 15min at 10000r/min, passes through molybdenum antimony resistance colorimetric method
It measures phosphorus decomposing amount (water-soluble phosphorus content).As shown in figure 3, available phosphorus content was just dramatically increased at second day, incrementss are
Between 420.95-433.94mg/L, third day is little compared with second day increments, incrementss 34.65-111.65mg/L, third
Phosphorus incrementss are basically unchanged in solution after it, and the phosphorus decomposing amount of X-P18 bacterial strain is 416.62-534.52mg/L.Dissolving phosphor and dissolving potassium bacterium X-
P18 dissolving P capacity with higher.
Embodiment 3: the ability of dissolving potassium measurement of bacterial strain.
100ml inorganic potassium fluid nutrient medium is separately added into 250ml triangular flask, medium component is sucrose 10g, sulfuric acid
Magnesium 0.5g, sulfate of ammoniac 0.5g, calcium carbonate 0.1g, sodium dihydrogen phosphate 3.14g, ferric sesquichloride 0.005g, feldspar in powder 3g, water
1000ml.The bacteria suspension that 2ml is prepared with sterile water is inoculated, while doing and not being inoculated with control (2ml sterile water is added in culture medium),
All processing carry out 3 repetitions.At 30 DEG C, 160rpm vibrates 7d respectively, and culture solution is centrifuged 15min at 10000rpm,
Pass through atomic absorption measuring potassium decomposing amount (water-soluble potassium content).As shown in figure 4, a few days ago incrementss are negative value, it may be possible to because
It is greater than the effective potassium total amount for decomposing release for effective potassium total amount that X-P18 bacterium is absorbed and utilized.From third day to the 7th day, potassium increases
Dosage becomes positive value, and curve is in rising trend, increases to 3.48mg/L from 0.12mg/L.Incrementss are 2.78-3.48mg/L.Solution
Phosphorus potassium solubilizing bacteria X-P18 has certain ability of dissolving potassium.
Embodiment 4: strain idenfication.
16sRNA sequence analysis: PCR amplification uses bacterium 16sRNA universal primer:
27F(5’-AGAGTTTGATCMTGGCTCAG-3’)
1492R(5’-GGTTACCTTGTTACGACTT-3’)
PCR reaction system (2.5 μ L): 5 × amplification buffer (5 μ L), genomic DNA (0.1 μ L),
DNTP (2 μ L), primers F (0.5 μ L), primer R (0.5 μ L), Taq archaeal dna polymerase (0.25 μ L), deionized water
(16.65μL).Reaction condition: 98 DEG C of initial denaturation 10min, 98 DEG C of deformation 10s, 58 DEG C of annealing 10s, 72 DEG C of extension 90s, totally 30
Circulation, 72 DEG C sufficiently extend 10min.
16S rDNA major part sequence is measured, as shown in SEQ ID No:1.The BLAST of the column website NCBI will be sequenced
The comparison of base sequence is carried out, constructs the systematic evolution tree based on 16S rDNA complete sequence, as shown in Figure 5.The result shows that: bacterium
It is homologous that strain with Bei Laisi bacillus reaches 98%.So assert that the present invention uses Bei Laisi bacillus (Bacillus
Velezensis), specific location Bei Laisi bacillus velezensis X-P18.
Embodiment 5: the preparation of phosphorus decomposing microbial inoculum
It will be stored in -20 DEG C of phosphate solubilizing bacteria Bacillus velezensis X-P18 activation, is coated on phosphorus decomposing solid medium
On, culture 16~for 24 hours；Strain on picking phosphorus decomposing culture medium purifies scribing line, culture 16 on fresh phosphorus decomposing solid medium
~for 24 hours；Single colonie on picking phosphorus decomposing solid medium, is inoculated in 15ml fermentation medium, and 30 DEG C of cultures 16~for 24 hours, then
Expand culture in fermentation medium, is 10 to bacterial concentration8-109When CFU/g, phosphorus decomposing microbial inoculum, amount of application 1.3-2.0L/ are obtained
m2。
The wherein phosphorus decomposing solid medium, component are as follows: glucose 5g, tricalcium phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac
0.5g, calcium carbonate 0.1g, potassium chloride 1.7g, ferric sesquichloride 0.005g, agar 20g, water 1000ml.
Fermentation medium are as follows: glucose 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, calcium carbonate 0.1g,
Ferric sesquichloride 0.005g, water 1000ml.
Embodiment 6: the preparation of potassium decomposing microbial inoculum
It will be stored in -20 DEG C of potassium solubilizing bacteria Bacillus velezensis X-P18 activation, is coated on potassium decomposing solid medium
On, culture 16~for 24 hours；Strain on picking potassium decomposing solid medium purifies scribing line, training on fresh potassium decomposing solid medium
Feeding 16~for 24 hours；Single colonie on picking potassium decomposing solid medium, is inoculated in 15ml fermentation medium, and 30 DEG C of cultures 16~
For 24 hours, expand culture in fermentation medium, be 10 to bacterial concentration8-109When CFU/g, potassium decomposing microbial inoculum is obtained, amount of application is
1.3-2.0L/m2。
The wherein potassium decomposing solid medium, component are as follows: sucrose 5g, feldspar in powder 3g, magnesium sulfate 0.5g, sulfate of ammoniac
0.5g, sodium dihydrogen phosphate 3.14g, calcium carbonate 0.1g, ferric sesquichloride 0.005g, agar 20g, water 1000ml.
Fermentation medium are as follows: glucose 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, calcium carbonate 0.1g,
Ferric sesquichloride 0.005g, water 1000ml.
Embodiment 7: the growth characteristics of bacterial strain.
The LB culture medium that salinity mass percent is 2%, 4%, 6%, 8%, 10% is prepared, each gradient is in each test tube
5ml culture medium is dispensed, and is sterilized after doing blank control, X-P18 bacterium solution is then inoculated with, is cultivated in 30 DEG C, 160r/min shaking table
1-3 days, observe whether it can grow.
The NaOH solution for preparing 1mol/L is used to deploy the pH value of LB culture medium, and LB solution and NaOH solution are sterilized, in
The LB solution that pH is 8,9,10,11 is deployed in super-clean bench, each gradient dispenses 5ml culture medium in each sterilizing test tubes, and does sky
White control is cultivated 1-3 days in 30 DEG C, 160r/min shaking table, observes whether it can grow.
100ml LB culture medium is prepared, 5ml culture medium is dispensed in 8 test tubes, in super-clean bench after inoculating strain, respectively
It is put into 20 DEG C, 30 DEG C, 40 DEG C, 160r/min culture 1-3d in 50 DEG C of shaking table with blank control, whether observation bacterial strain can grow.
The growth characteristics of table 1.X-P18
| PH range | Whether can grow | Salinity range | Whether can grow | Temperature (DEG C) | Whether can grow |
| 8 | + | 2% | + | 20 | + |
| 9 | + | 4% | + | 30 | + |
| 10 | + | 6% | + | 40 | + |
| 11 | + | 8% | + | 50 | + |
| 12 | - | 10% | - |
As shown in Table 1, X-P18 bacterial strain can be grown in the case where pH is the range within 8-11, salinity mass percent 8%,
Growth temperature range is larger, can grow at 20-50 DEG C.
Embodiment 8: crop yield can be improved in dissolving phosphor and dissolving potassium microbial inoculum.
Flowerpot bore 140mm, the high 115mm used is tested, every basin fills soil 650g, rate of fertilizer 63.7g/m2Phosphoric acid
Tricalcium, 11.7g/m2Potassium chloride, 13.7g/m2Urea, bacterium solution amount of application are 1.3L/m2, mix fertilizer and soil thoroughly dress basin, then broadcast
Fungus scattering liquid (sterile water wash 3 times) is sowed after standing for 1 night, and every basin pakchoi sows 6 seeds (percentage of seedgermination 98%),
Top earthing 0.5cm, thinning after two weeks to Chinese cabbage growth, adds bacterium solution along plant root again to 3 after germination
(sterile water wash 3 times), bacterium solution amount of application are 1.3L/m2.Using ?leaf palm-leaf fun Chinese cabbage as experimental subjects, wherein
CK group: normal soil+fertilizer
A1 group: normal soil+fertilizer+X-P18 microbial inoculum
Each processing group harvests, plant is cut along root, is eluted with water, biomass is measured for the 30th day in plant growth.
Growth characteristics and yield effect of the 2. dissolving phosphor and dissolving potassium microbial inoculum of table to Chinese cabbage
| Experimental group | Pakchoi plant height (cm) | Pakchoi fresh weight (g/ basin) | Increase production compared with CK |
| CK | 19.63±0.27a | 26.79±0.88a | - |
| A1 | 21.95±0.42b | 32.83±0.57b | 22.55% |
As shown in Table 2, dissolving phosphor and dissolving potassium microbial inoculum can significantly improve pakchoi plant height and fresh weight, increase production with microbial inoculum comparison is not added
22.55%, illustrate that dissolving phosphor and dissolving potassium microbial inoculum can significantly improve Growth of Cabbage characteristic and yield.
Embodiment 9: dissolving phosphor and dissolving potassium microbial inoculum can reduce the application of chemical fertilizer.
A kind of efficient phosphate-solubilizing potassium solubilizing bacteria promotion crop growth, the method for reducing chemical fertilizer application test the flowerpot mouth of use
Diameter 140mm, high 115mm, every basin fill soil 650g, rate of fertilizer 63.7g/m2Tricalcium phosphate, 11.7g/m2Potassium chloride,
13.7g/m2Urea, bacterium solution amount of application are 1.3L/m2, mix fertilizer and soil thoroughly dress basin, then sow bacterium solution (sterile water wash 3
All over), it is sowed after standing for 1 night, every basin pakchoi sows 6 seeds (percentage of seedgermination 98%), top earthing 0.5cm, wait plant
Thinning after two weeks to Chinese cabbage growth, adds bacterium solution (sterile water wash 3 times), bacterium solution along plant root again to 3 after son germination
Amount of application is 1.3L/m2.Using ?leaf palm-leaf fun Chinese cabbage as experimental subjects, wherein
CK group: normal soil+fertilizer
A1 group: normal soil+fertilizer+X-P18 microbial inoculum
A2 group :+70% fertilizer+X-P18 microbial inoculum of normal soil
Each processing group harvests, plant is cut along root, is eluted with water, biomass is measured for the 30th day in plant growth.
3. dissolving phosphor and dissolving potassium microbial inoculum of table and chemical fertilizer are to potting pakchoi characteristic and yield effect
| Experimental group | Pakchoi plant height (cm) | Pakchoi fresh weight (g/ basin) | Increase production compared with CK |
| CK | 19.75±0.44b | 26.34±0.59c | - |
| A1 | 22.66±0.37a | 32.95±0.66a | 25.09% |
| A2 | 20.70±0.6b | 30.70±0.40b | 16.55% |
As shown in Table 3, dissolving phosphor and dissolving potassium microbial inoculum is mixed with fertilizer and is applied, and can promote the root development of pakchoi, to pakchoi strain
High, yield has facilitation, can increase production 25.09%.When subtracting the fertilizer for applying 30% mass, still the yield of Chinese cabbage is mentioned
Height can increase production 16.55%, this illustrates that X-P18 microbial inoculum can effectively reduce the amount of application of chemical fertilizer.
Embodiment 10: dissolving phosphor and dissolving potassium microbial inoculum improves crop yield in salt-soda soil
A kind of efficient phosphate-solubilizing potassium solubilizing bacteria promotes the method for crop growth in salt-soda soil, tests the flowerpot bore of use
140mm, high 115mm, every basin fill soil 650g, rate of fertilizer 63.7g/m2Tricalcium phosphate, 11.7g/m2Potassium chloride, 13.7g/
m2Urea, bacterium solution amount of application are 1.3L/m2, mix fertilizer and soil thoroughly dress basin, then sow bacterium solution (sterile water wash 3 times), stand
It is sowed after 1 night, every basin pakchoi sows 6 seeds (percentage of seedgermination 98%), top earthing 0.5cm, after germination
Thinning after two weeks to Chinese cabbage growth, adds bacterium solution (sterile water wash 3 times) along plant root again, bacterium solution amount of application is to 3
1.3L/m2.Alkaline land soil property is that pH is 8.3, salinity 0.5%.Using ?leaf palm-leaf fun Chinese cabbage as experimental subjects, wherein
CK group: salt affected soil+fertilizer
A1 group: normal soil+fertilizer
A2 group: normal soil+fertilizer+X-P18 microbial inoculum
Each processing group harvests, plant is cut along root, is eluted with water, biomass is measured for the 30th day in plant growth.
4. dissolving phosphor and dissolving potassium microbial inoculum of table and chemical fertilizer are to salt affected soil potting pakchoi characteristic and yield effect
| Experimental group | Pakchoi plant height (cm) | Pakchoi fresh weight (g/ basin) | Increase production compared with CK |
| CK | 17.70±0.15b | 22.68±0.50b | - |
| A1 | 20.00±0.30a | 28.02±0.80b | 23.54% |
| A2 | 20.84±0.48a | 29.94±0.38a | 32.01% |
As shown in Table 4, dissolving phosphor and dissolving potassium microbial inoculum can significantly improve pakchoi plant height and fresh weight in alkaline land soil, and be not added
Microbial inoculum comparison volume increase 32.01%, increases production 6.85% compared with normal soil, illustrates that dissolving phosphor and dissolving potassium microbial inoculum can be in alkaline land soil
Also the growth-promoting functions to pakchoi are played.
Embodiment 11: dissolving phosphor and dissolving potassium microbial inoculum and charcoal combination improve crop yield
A kind of method of efficient phosphate-solubilizing potassium solubilizing bacteria and charcoal combination promotion crop growth, tests the flowerpot bore of use
140mm, high 115mm, every basin fill soil 650g, rate of fertilizer 63.7g/m2Tricalcium phosphate, 11.7g/m2Potassium chloride, 13.7g/
m2Urea, bacterium solution amount of application are 1.3L/m2, mix fertilizer and soil thoroughly dress basin, then sow bacterium solution (sterile water wash 3 times), stand
It is sowed after 1 night, every basin pakchoi sows 6 seeds (percentage of seedgermination 98%), top earthing 0.5cm, after germination
Thinning after two weeks to Chinese cabbage growth, adds bacterium solution (sterile water wash 3 times) along plant root again, bacterium solution amount of application is to 3
1.3L/m2.Charcoal dosage is 1.5kg/m2(charcoal can be with peanut shell, soybean stalk, straw, rice husk, corncob etc.
Raw material are made).Using ?leaf palm-leaf fun Chinese cabbage as experimental subjects, wherein
CK group: normal soil+fertilizer
A1 group: normal soil+charcoal+fertilizer
A2 group: normal soil+fertilizer+X-P18 microbial inoculum
A3 group: normal soil+charcoal+fertilizer+X-P18 microbial inoculum
Each processing group harvests, plant is cut along root, is eluted with water, biomass is measured for the 30th day in plant growth.
5. dissolving phosphor and dissolving potassium microbial inoculum of table and charcoal are combined to pakchoi characteristic and yield effect
| Experimental group | Pakchoi plant height (cm) | Pakchoi fresh weight (g/ basin) | Increase production compared with CK |
| CK | 19.90±0.30b | 27.34±1.00c | - |
| A1 | 20.21±0.31b | 29.37±0.75b | 7.43% |
| A2 | 22.45±0.35a | 33.70±0.30a | 23.26% |
| A3 | 22.78±0.45a | 36.99±1.24a | 35.30% |
As shown in Table 5, charcoal can increase the yield of pakchoi to a certain extent, and dissolving phosphor and dissolving potassium microbial inoculum and charcoal
It is mixed to apply, it can more promote the root development of pakchoi, have facilitation to yield of pakchoi, 35.30% can be increased production, than individually applying
It is more significant with dissolving phosphor and dissolving potassium bacterial manure effect.
Sequence table
<110>Nanjing University of Technology
<120>one plant heights imitate dissolving phosphor and dissolving potassium bacterium and its application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 988
<212> DNA
<213>Bei Laisi bacillus (Bacillus velezensis)
<400> 1
ttgatgttat ctgcaagtcg agcggacaga tgggagcttg ctccctgatg ttagcggcgg 60
acgggtgagt aacacgtggg taacctgcct gtaagactgg gataactccg ggaaaccggg 120
gctaataccg gatggttgtt tgaaccgcat ggttcaaaca taaaaggtgg cttcggctac 180
cacttacaga tggacccgcg gcgcattagc tagttggtga ggtaacggct caccaaggca 240
acgatgcgta gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga 300
ctcctacggg aggcagcagt agggaatctt ccgcaatgga cgaaagtctg acggagcaac 360
gccgcgtgag tgatgaaggt tttcggatcg taaagctctg ttgttaggga agaacaagta 420
ccgttcgaat agggcggtac cttgacggta cctaaccaga aagccacggc taactacgtg 480
ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gaattattgg gcgtaaaggg 540
ctcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt 600
ggaaactggg gaacttgagt gcagaagagg agagtggaat tccacgtgta gcggtgaaat 660
gcgtagagat gtggaggaac accagtggcg aaggcgactc tctggtctgt aactgacgct 720
gaggagcgaa agcgtggggg agcgaacagg attagatacc ctggtagtcc acgccgtaaa 780
cgatgagtgc taagtgttag ggggtttccg ccccttagtg ctgcagctaa cgcattaagc 840
actccgccct ggggagtacg gtcgcaagac tggaaactca aggaattgac ggggggcccg 900
cacaagcgtg gagcatgtgg tttaattcga accacgcgaa gaaccttacc agtcttgaca 960
tcctctgaca atccctagag atagacgc 988
Claims (7)
1. a plant height imitates dissolving phosphor and dissolving potassium bacterium, classification naming is Bei Laisi bacillus (Bacillus velezensis), bacterial strain
Number X-P18, has been preserved in China typical culture collection center, and deposit number is CCTCC NO:M 2019317, preservation date
For on April 29th, 2019.
2. application of the efficient phosphate-solubilizing potassium solubilizing bacteria described in claim 1 in soil phosphorus decomposing and/or potassium decomposing.
3. application according to claim 2, which is characterized in that efficient phosphate-solubilizing potassium solubilizing bacteria is prepared as follows into microbial inoculum
For the phosphorus decomposing and/or potassium decomposing of soil, amount of application 1.3-2.0L/m2:
It will be stored in -20 DEG C of dissolving phosphor and dissolving potassium bacterium activation, is coated on phosphorus decomposing solid medium, culture 16~for 24 hours；Picking phosphorus decomposing is solid
Strain on body culture medium is purified on fresh phosphorus decomposing solid medium and is crossed, and culture 16~for 24 hours；The training of picking phosphorus decomposing solid
The single colonie on base is supported, is inoculated in fermentation medium, culture 16~for 24 hours, expand culture in fermentation medium, to bacterium solution
Concentration is 108-109When CFU/g, phosphorus decomposing of the fermentation liquid for soil is obtained, alternatively,
It will be stored in -20 DEG C of dissolving phosphor and dissolving potassium bacterium activation, is coated on potassium decomposing solid medium, culture 16~for 24 hours；Picking potassium decomposing is solid
Strain on body culture medium is purified on fresh potassium decomposing solid medium and is crossed, and culture 16~for 24 hours；The training of picking potassium decomposing solid
The single colonie on base is supported, is inoculated in fermentation medium, culture 16~for 24 hours, expand culture in fermentation medium, to bacterium solution
Concentration is 108-109When CFU/g, potassium decomposing of the fermentation liquid for soil is obtained.
4. application according to claim 3, which is characterized in that the phosphorus decomposing solid culture based formulas are as follows: glucose 5g,
Tricalcium phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, potassium chloride 1.7g, calcium carbonate 0.1g, ferric sesquichloride 0.005g, agar
20g, water 1000ml.
Fermentation medium are as follows: glucose 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, calcium carbonate 0.1g, chlorination
High-speed rail 0.005g, water 1000ml.
Potassium decomposing solid culture based formulas are as follows: sucrose 5g, feldspar in powder 3g, magnesium sulfate 0.5g, sulfate of ammoniac 0.5g, sodium dihydrogen phosphate
3.14g, calcium carbonate 0.1g, ferric sesquichloride 0.005g, agar 20g, water 1000ml.
5. efficient phosphate-solubilizing potassium solubilizing bacteria described in claim 1 is promoting plant growth, is reducing the application in fertilizer amount.
6. application according to claim 5, which is characterized in that after being uniformly sprinkled into vegetable seeds into soil, top earthing
0.5cm。
7. application according to claim 5 or 6, which is characterized in that into soil, sowing fertilizers are 63.7g/m2Tricresyl phosphate
Calcium, 11.7g/m2Potassium chloride, 13.7g/m2Urea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910475323.9A CN110184220B (en) | 2019-05-31 | 2019-05-31 | Efficient phosphate and potassium solubilizing bacterium and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910475323.9A CN110184220B (en) | 2019-05-31 | 2019-05-31 | Efficient phosphate and potassium solubilizing bacterium and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110184220A true CN110184220A (en) | 2019-08-30 |
| CN110184220B CN110184220B (en) | 2020-07-31 |
Family
ID=67719825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910475323.9A Active CN110184220B (en) | 2019-05-31 | 2019-05-31 | Efficient phosphate and potassium solubilizing bacterium and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110184220B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269039A (en) * | 2020-03-27 | 2020-06-12 | 常州市研核生物科技有限公司 | Microbial fertilizer and preparation method thereof |
| CN111621448A (en) * | 2020-07-03 | 2020-09-04 | 沈阳农业大学 | Bacillus belgii SN-1 and method for producing exopolysaccharides through fermentation of bacillus belgii SN-1 |
| CN112322518A (en) * | 2020-10-15 | 2021-02-05 | 华南农业大学 | Bacillus alpinus with potassium-dissolving effect and application thereof |
| CN112940962A (en) * | 2021-01-07 | 2021-06-11 | 华南农业大学 | Bacillus belgii and application thereof in improving copper pollution in water body |
| CN113215044A (en) * | 2021-05-13 | 2021-08-06 | 安徽农业大学 | Potassium-decomposing bacterium and application thereof |
| CN114196563A (en) * | 2021-08-25 | 2022-03-18 | 广西民族大学 | Bacillus belgii and application thereof |
| CN116023180A (en) * | 2022-05-11 | 2023-04-28 | 南京环境集团有限公司 | Method for preparing microbial fertilizer by mixing biogas residues and kitchen waste with aerobic compost |
| CN116622576A (en) * | 2023-05-26 | 2023-08-22 | 中国水稻研究所 | Mars agricultural bacterium, composite microbial inoculant and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112011487B (en) * | 2020-09-14 | 2021-12-21 | 河南农业大学 | Aroma-like bacterial strain C40 capable of degrading phenol and having growth promoting function and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232538A (en) * | 2014-09-03 | 2014-12-24 | 南京聚肽高科农业有限公司 | Efficient potassium bacterium and application thereof |
| CN104263679A (en) * | 2014-09-03 | 2015-01-07 | 南京聚肽高科农业有限公司 | High-efficiency phosphate-solubilizing bacteria and application thereof |
| CN104276882A (en) * | 2014-09-03 | 2015-01-14 | 南京聚肽高科农业有限公司 | Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist |
-
2019
- 2019-05-31 CN CN201910475323.9A patent/CN110184220B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232538A (en) * | 2014-09-03 | 2014-12-24 | 南京聚肽高科农业有限公司 | Efficient potassium bacterium and application thereof |
| CN104263679A (en) * | 2014-09-03 | 2015-01-07 | 南京聚肽高科农业有限公司 | High-efficiency phosphate-solubilizing bacteria and application thereof |
| CN104276882A (en) * | 2014-09-03 | 2015-01-14 | 南京聚肽高科农业有限公司 | Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist |
Non-Patent Citations (2)
| Title |
|---|
| KYEONG HWANGBO ET AL.: "Complete Genome Sequence of Bacillus velezensis CBMB205, a Phosphate-Solubilizing Bacterium Isolated from the Rhizoplane of Rice in the Republic of Korea", 《GENOME ANNOUNCEMENTS》 * |
| 王海德: "溶磷解钾菌株的筛选及培养基优化", 《中国优秀硕士学位论文全文数据库(电子期刊)工程科技辑》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269039A (en) * | 2020-03-27 | 2020-06-12 | 常州市研核生物科技有限公司 | Microbial fertilizer and preparation method thereof |
| CN111621448A (en) * | 2020-07-03 | 2020-09-04 | 沈阳农业大学 | Bacillus belgii SN-1 and method for producing exopolysaccharides through fermentation of bacillus belgii SN-1 |
| CN112322518A (en) * | 2020-10-15 | 2021-02-05 | 华南农业大学 | Bacillus alpinus with potassium-dissolving effect and application thereof |
| CN112940962A (en) * | 2021-01-07 | 2021-06-11 | 华南农业大学 | Bacillus belgii and application thereof in improving copper pollution in water body |
| CN112940962B (en) * | 2021-01-07 | 2021-09-14 | 华南农业大学 | Bacillus belgii and application thereof in improving copper pollution in water body |
| CN113215044A (en) * | 2021-05-13 | 2021-08-06 | 安徽农业大学 | Potassium-decomposing bacterium and application thereof |
| CN114196563A (en) * | 2021-08-25 | 2022-03-18 | 广西民族大学 | Bacillus belgii and application thereof |
| CN116023180A (en) * | 2022-05-11 | 2023-04-28 | 南京环境集团有限公司 | Method for preparing microbial fertilizer by mixing biogas residues and kitchen waste with aerobic compost |
| CN116622576A (en) * | 2023-05-26 | 2023-08-22 | 中国水稻研究所 | Mars agricultural bacterium, composite microbial inoculant and application thereof |
| CN116622576B (en) * | 2023-05-26 | 2024-03-26 | 中国水稻研究所 | Mars agricultural bacterium, composite microbial inoculant and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110184220B (en) | 2020-07-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110184220A (en) | One plant height imitates dissolving phosphor and dissolving potassium bacterium and its application | |
| CN105838644B (en) | Complex micro organism fungicide and bacterial manure and preparation method thereof and the application in reparation salt affected soil | |
| CN104672033B (en) | Amino acid bio-organic fertilizer and its production and use | |
| CN100572337C (en) | A kind of whole-fertilizer compound fertilizer and preparation method thereof | |
| CN101225367B (en) | Microorganism, microorganism phosphatic fertilizer manufactured thereby and manufacturing method thereof | |
| CN102344812B (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN102432399B (en) | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN105936881B (en) | One kind is for alignic thermophilic sugared bacillus and its application method of degrading | |
| CN103011961B (en) | Active organic bacterial manure and preparation method thereof | |
| CN104262047A (en) | High-activity humic acid composite microorganism fertilizer and preparation method thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN104498040B (en) | Application of the bacillus amyloliquefaciens as soil conditioner | |
| CN106495933B (en) | A kind of cucumber seedling-raising substrate and preparation method thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN102240664A (en) | Method for restoring soil polluted by manganese | |
| CN106518535A (en) | Full-nutrition ecological substrate nutrient soil and preparation method | |
| CN109762765A (en) | A kind of decomposed solid fermentation microbial inoculum and its application in agricultural wastes | |
| CN108017446A (en) | The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium | |
| CN103819282A (en) | Orange peel dregs seedling raising substrate and preparation method thereof | |
| CN110437000A (en) | A kind of microbial manure and its preparation method and application | |
| CN104293719B (en) | Fast decomposing agent for fermentation bed aging padding, organic fertilizer and production method of organic fertilizer | |
| CN1974490A (en) | Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure | |
| CN105985195A (en) | Organic liquid fertilizer and preparation method thereof | |
| CN102942420B (en) | Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |