CN1974490A - Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure - Google Patents
Process of fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure Download PDFInfo
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Abstract
The present invention relates to production of fulvic acid, and is especially the process of producing fulvic acid bacterial manure with waste from farm and sideline product processing and through fermentation and conversion. Through selecting and breeding bacteria strain, proper solid-semi-solid fermentation process, and re-compounding fulvic acid and nitrogen fertilizer, phosphate fertilizer and potash fertilizer bacteria, the present invention produces the fulvic acid bacterial manure with excellent synergistic effects of regulating the opening of foliage air pore, reducing water evaporation, retaining water, promoting root system growth and raising the drought fighting capacity of crop.
Description
Technical field
The present invention relates to a kind of method of utilizing microbial solid-semisolid fermentation explained hereafter biochemical fulvic acid.Especially a kind of all kinds of waste from farm and sideline product processing such as pomace, stalk that utilize change into the production technique of fulvic acid bacterial manure after many bacterial classifications of microorganism solid-semi-solid mixed fermentation.
Background technology
" pomace " is meant the fruit juice factory back waste residue that produces of squeezing the juice among the present invention, and its gross protein value is lower, and weight percent is only about 1～2%, but sugar and organic acid content are higher.In addition, also contain major elements such as more rich calcium, phosphorus, potassium, iron, manganese, sulphur and trace element etc.Along with the emergence of China's fruit juice processing industry, produced the pomace of increasing year by year in recent years, be used as the feed except a small amount of, these residues mainly go out of use, and form new industrial or agricultural rubbish, and new problem and burden are brought to environmental protection in the back of rotting.
Biochemical fulvic acid (BAF) is a method of utilizing biotechnology, is raw material with the agricultural tankage, the product that the decomposition of the biology that process is complicated under the microbial fermentation effect, chemistry and building-up reactions generate.The biochemical fulvic acid as fertilizer sources uses, and compares with humic acid, and it has following characteristics of three aspects:
1, molecular weight is little, is utilized by plant absorbing easily;
2, functional group content is many, the physiologically active height;
3, can be directly water-soluble, it is acid that its aqueous solution is.
This of xanthohumic acid three big features the agricultural, pharmaceutically practical application effect is more superior than humic acid.
Biochemical fulvic acid (BFA) is a microbial fermentation and generating, it is the very complicated mixture of a kind of composition, contain in a large number biologically active substance and nutritions such as the influential enzyme of growing process, amino acid, VITAMIN and trace elements, to promoting plant-growth, growing remarkable efficacy is arranged.BFA has good water-solubility, in soil, can strengthen the surge capability of soil by complexing, chelating, adsorption, improve guarantor's fertilizer, the water retention capacity of soil, improve the ventilation property of soil and the activity of soil microorganisms, increase effective flora quantity and reach the effect of improving the soil.BFA is the material of finding at present of regulating blade face pore opening degree most effectively, it can dwindle blade face pore opening degree, reduce moisture transpiration, make plant and Soil conservation more water, promote the root system development growth simultaneously, improve the root system vigor and make crop absorb more water and nutriment that the two complements each other, increase income and decrease expenditure, reach the purpose that improves crop drought resistance water saving ability.BFA is a kind of good plant-growth regulator; BFA has stronger ionic bond ability, can significantly improve plant to trace elements absorbed and running ability.Composite with chemical fertilizer, can obviously increase fertilizer efficiency, reduce fertilizer amount, reduce loss of chemical fertilizer.
At present, produce xanthohumic acid both at home and abroad and mainly contain following method:
1, utilize coal (peat, brown coal, weathered coal) to produce xanthohumic acid.
2, chemosynthesis xanthohumic acid.
3, biological fermentation is produced xanthohumic acid.
First kind of method of utilizing coal (peat, brown coal, weathered coal) to produce xanthohumic acid, need coal to be degraded with chemical process, in its degradation process, need to consume chemical substances such as a large amount of acid, alkali, what have also needs violent condition such as high temperature, high pressure and strict process and specific installation, consume lot of energy, produce waste water, waste gas simultaneously, environment is polluted.
External many people adopt the method for second kind of chemosynthesis xanthohumic acid, the generative process of simulation natural humus acid, utilize polyphenol, quinone, carbohydrate, urea, xylogen or itrogenous organic substance etc. to carry out the synthetic xanthohumic acid of oxidative condensation, cost is high and be difficult to scale operation.
Utilize agricultural and sideline processing waste to produce biochemical fulvic acid by microbial fermentation, can make refuse obtain utilizing, can not pollute environment, raw material is easy to get, and cost is low.
Summary of the invention
The present invention is to be raw material with all kinds of waste from farm and sideline product processing such as pomace, stalks, adopt many bacterial classifications of microorganism solid-semi-solid mixed fermentation technology to produce the production technique of fulvic acid bacterial manure, both solve the problem of environmental pollution that a large amount of pomaces rot to bring, again it had been become the biological organic fungi-manure of super quality and competitive price.
The present invention realizes by the following technical solutions:
With aspergillus niger (Aspergillus niger), viride (Trichoderma viride), Bacillus subtillis (Bacillus subtilis), false pseudomonas bacillus (Pseudomonas sp.), distillery yeast (Saccharomycescerevisiae), candiyeast (Candida sp.) respectively through test tube cultivation, shake-flask culture, seed tank culture, insert the fermentor tank liquid culture respectively by 10% inoculum size, culture temperature is 25～35 ℃, incubation time is 24～72 hours, cultivate into production high density thalline bacteria suspension, viable count is 〉=10
8Individual/ml.
Lactobacillus delbruckii is that the static anaerobism of liquid is cultivated, and progressively classification enlarged culturing, culture temperature are 40～60 ℃, and incubation time is 24～48 hours, and viable count is 〉=10
8Individual/ml.。
With fermentor tank gained aspergillus niger, viride, Bacillus subtillis, false pseudomonas bacillus high density thalline bacteria suspension, with 0.5～2: 0.5～2: 0.5～2: 0.5～2 volume ratio inserts the sterilization solid medium respectively, make after stirring and produce that to use solid mix bacterium agent, viable count be 〉=10
8Individual/g.Described solid medium can be a bran mass.
Aspergillus niger, viride, Bacillus subtillis, false pseudomonas bacillus high density thalline bacteria suspension are preferably with 1: 1: 1: 1 volume ratio inserts the sterilization solid medium.
Because material variety and fruit picking phase difference, the pH value of material distributes very big, the apple pomace pH value 5.5 in ripening stage, and immature apple pomace pH value 3.5～4, inoculation is preceding earlier with potassium primary phosphate (KH
2PO
4) or lime carbonate (CaCO
3) pH value of described material is transferred to 5.5.Need not to regulate pH value for PH greater than 5.5 material.
To use the urea of solid mix bacterium agent and 1～2% to insert pretreated material with the described production of weight of material 1～5%, and stir and carry out solid fermentation, 25～45 ℃ of leavening temperatures, fermentation time be 5～10 days, and viable count is 〉=10
8Individual/g.
Lactobacillus delbruckii, distillery yeast, candiyeast bacteria suspension, three kinds of bacteria suspensions are by 0.5～2: 0.5～2: 0.5～2 volume ratio is mixed into to produce uses the mixed bacterium suspension; Preferably mix with 1: 1: 1 volume ratio.
After the material solid fermentation is finished, tunning is semi-solid state because of there being big quantity of fluid to ooze out, be that 1～5% described production inserts the semisolid fermentation product with the mixed bacterium suspension with tunning weight count number, stir and carry out semisolid fermentation, leavening temperature is 20～40 ℃, and fermentation time is 5～10 days.
Reduce to below 1% when the fermented liquid sugar, pH value is reduced to below 3, and viable count is 〉=10
8/ ml, fermentation is promptly finished.Tunning is carried out solid-liquid separation by plate filter, and gained filtrate is the liquid fulvic acid bacterial manure, and filter residue can be used as the solid fulvic acid bacterial manure.Filter residue is through drying or drying, and moisture is reduced to about 13%, is squeezed into desired shape, size particles with single-lead-screw extruding briquetting machine, through weighing, packing, promptly becomes the solid fulvic acid bacterial manure.
The used bacterial classification of the present invention all is known bacterial classification, can obtain by known screening, commercial means or other approach, candiyeast and pseudomonas all are bacterial classifications commonly used in this area, as candiyeast can be candida tropicalis (Candida tropicalis), and pseudomonas can be fluorescent pseudomonas (Pseudomonas fluorescens).
Each used substratum all is the conventional substratum of the described microorganism of known suitable cultivation in the cultural methods such as test tube cultivation of the present invention, shake-flask culture, seeding tank training, fermentor cultivation, enlarged culturing.
Above treatment process both had been applicable to that fruit juice factory squeezed the juice the back without the fresh fruit slag of any processing, also was applicable to agricultural byproducts tankage such as pomace after handling with the fresh fruit slag oven dry or with additive method, stalk.
The invention has the advantages that:
1, adopts modern advanced strain improvement technology, selected and efficiently to have transformed all kinds of production bacterial classifications that pomace becomes biochemical fulvic acid, reach highly active vinelandii, phosphorus bacteria, potassium bacterium, guaranteed to produce the high quality of bacterial classification.
2, the process that generates under all kinds of microbial processs according to xanthohumic acid is designed and is fit to solid-semisolid zymotechnique that xanthohumic acid is produced, make all kinds of floras can high-quality under the suitable culture condition, fermentative production xanthohumic acid efficiently.
3, adopt advanced deep layer fermenting process; Solved a technology and the prescription difficult problem of mixed fermentation N.P.K bacterium, both saved equipment, can obtain effective viable bacteria body of lot of pure again, (the effective viable bacteria body of fermented liquid can reach 10
10Individual/ml), be that three kinds of bacterium synergy raising fertilizer efficiency lay the foundation.
4, worked out the compounded formula of biochemical fulvic acid and N.P.K bacterium, it is composite to carry out science at the special-purpose fertilizer of dissimilar crops, two biolobic material fertilizer can be organically combined, collaborative performance effect.This yet there are no analogous products in fertilizer industry at present.
5, owing to the inexpensive raw material of employing, good production bacterial classification, advanced technology, scientific formula, this project various product is compared production cost with similar other products and will be descended significantly, and action effect is then very remarkable.
6, the xanthohumic acid bacterial manure that obtains of the present invention is the material that can regulate blade face pore opening degree effectively, can play and dwindle blade face pore opening degree, reduce moisture transpiration, make plant and Soil conservation more water, promote the root system development growth simultaneously, reach the purpose that improves crop drought resistance water saving ability.
Embodiment
Example I:
20 tons of fermentative processing fresh apple slags, its main component is: carbohydrate about 20%, robust fibre 10～15%, water content 45%～55%, crude protein 5.6%, crude fat 6.2%, calcium 0.06%, phosphorus 0.05%, trace copper 11.8mg/kg, zinc 15.4mg/kg, manganese 14.0mg/kg, iron 158mg/kg, selenium 0.08mg/kg, aminoacids content 0.5%; PH value 4.5.
The preparation method:
(1), aspergillus niger, viride, Bacillus subtillis, Pseudomonas fluorescens, distillery yeast and candida tropicalis are cultivated through test tube respectively, well-known different production bacterial classifications adopts corresponding separately substratum, culture temperature is 25～35 ℃, and incubation time is 24～72 hours; Bacterial classification changed shake-flask culture over to after test tube was cultivated and finished, and culture temperature is 25～35 ℃, and rotating speed is 180～220 rev/mins, and incubation time is 24-48 hour; Bacterial classification inserted the seeding tank stir culture after shake-flask culture was finished, and culture temperature is 25～35 ℃, and incubation time is 24～48 hours; After seed tank culture is finished, insert the fermentor tank liquid culture by 10% weight inoculum size, culture temperature is 28～32 ℃, incubation time 36～42 hours; Aspergillus niger, viride, Bacillus subtillis, pseudomonas are cultivated gained high-concentration bacterial suspension respectively in 1: 1: 1: 1 ratio inserts the sterilization bran mass, makes to produce and uses the solid mix bacterium agent;
(2), lactobacillus delbruckii adopts the static culture method of liquid, progressively classification enlarged culturing, culture temperature are 50～54 ℃, each incubation time is 34～40 hours;
(3), utilize potassium primary phosphate (KH
2PO
4) pomace is transferred to pH value 5.5;
(4), will be that the urea of 2～4% described solid mix bacterium agent and 1～2% and modulated pomace stir and carry out solid fermentation with pomace weight count number, leavening temperature is 30～40 ℃, fermentation time 6～8 days;
(5), lactobacillus delbruckii, distillery yeast, candida tropicalis bacteria suspension, three kinds of bacteria suspensions become the mixed bacterium suspension by 1: 1: 1 mixed;
(6), after the pomace solid fermentation is finished, in the tunning because of there being big quantity of fluid to ooze out, be semi-solid state, to be 1～5% above-mentioned production mixed bacterium suspension access semisolid fermentation product this moment with tunning weight count number, stir and carry out semisolid fermentation, leavening temperature is 20～40 ℃, and fermentation time is 5～10 days.
(7), reduce to below 1%, pH value is reduced to below 3, and viable count is 〉=10 when the fermented liquid sugar
8Fermentation is finished during/ml.Tunning is carried out solid-liquid separation by plate filter, and gained filtrate is the liquid fulvic acid bacterial manure, and filter residue can be used as the solid fulvic acid bacterial manure.Filter residue is through drying or drying, and moisture is reduced to about 13%, is squeezed into desired shape, size particles with single-lead-screw extruding briquetting machine, through weighing, packing, promptly becomes the solid fulvic acid bacterial manure.
Liquid fulvic acid bacterial manure and solid fulvic acid bacterial manure main component are:
Liquid fulvic acid bacterial manure main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Viable count 〉=10
8/ ml.
Solid fulvic acid bacterial manure main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Organic 〉=40%; Viable count 〉=10
9/ g; Moisture≤13%; Granularity 2.5～4.5mm 〉=80%.
Example II:
20 tons of fermentative processing sweet sorghum straws, its main component is: crude protein 8.2% in the sweet sorghum seed, crude fat 2.6%, robust fibre 3.6%, ash content 3.9%, calcium 0.07%, phosphorus 0.24%; Juice containing amount is 70～80% in the cane, and it contains sugar hammer degree Bri is 17～21%, pH value 7.0.Be crushed to 〉=80% material particles diameter is 2.5-4.5mm.
The preparation method:
Aspergillus niger, viride, Bacillus subtillis, Pseudomonas fluorescens, distillery yeast and candida tropicalis are cultivated through test tube respectively, well-known different production bacterial classifications adopts corresponding separately substratum, the test tube culture temperature is 25～35 ℃, incubation time 24～72 hours; Bacterial classification changed over to and shakes bottle after test tube was cultivated and finished, and the shake-flask culture temperature is 25～35 ℃, and rotating speed is 180～220 rev/mins, incubation time 24～48 hours; Bacterial classification inserted the seeding tank stir culture after shake-flask culture was finished, and the seed tank culture temperature is 25～35 ℃, incubation time 24～48 hours; Insert the fermentor tank liquid culture by 10% weight inoculum size, culture temperature is 25-35 ℃, incubation time 24-72 hour; Aspergillus niger, viride, Bacillus subtillis, Pseudomonas fluorescens are cultivated gained high-concentration bacterial suspension respectively in 1: 1: 1: 1 ratio inserts the sterilization bran mass, makes to produce and uses the solid mix bacterium agent;
(2), lactobacillus delbruckii adopts progressively classification enlarged culturing of the static culture method of liquid, culture temperature is 50～54 ℃, incubation time is 34～40 hours;
(3), will to be 2～4% described production with the urea of solid mix bacterium agent and 1～2% and modulated stalk stir carries out solid fermentation with stalk weight count number, leavening temperature is 30～45 ℃, fermentation time 6～10 days;
(4), lactobacillus delbruckii, distillery yeast, candiyeast bacteria suspension, three kinds of bacteria suspensions become the mixed bacterium suspension by 1: 1: 1 mixed;
(5), after the stalk solid fermentation is finished, there is big quantity of fluid to ooze out in the tunning, be semi-solid state, to be 1～5% above-mentioned production mixed bacterium suspension access semisolid fermentation product this moment with tunning weight count number, stir and carry out semisolid fermentation, leavening temperature is 20～40 ℃, and fermentation time is 5～10 days;
(6), the fermented liquid sugar reduces to below 1%, pH value is reduced to below 3, viable count is 〉=10
8Fermentation is finished during/ml.Tunning is carried out solid-liquid separation by plate filter, and gained filtrate is the liquid fulvic acid bacterial manure, and filter residue can be used as the solid fulvic acid bacterial manure.Filter residue is through drying or drying, and moisture content should be squeezed into desired shape, size particles with single-lead-screw extruding briquetting machine less than 13%, through weighing, packing, promptly becomes the solid fulvic acid bacterial manure.
Liquid and solid xanthohumic acid main component are:
Liquid xanthohumic acid main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Viable count 〉=10
8/ ml.
Solid xanthohumic acid main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Viable count 〉=10
9/ g; Organic 〉=40%; Moisture≤13%; Granularity 2.5～4.5mm 〉=80%.
The application test of the xanthohumic acid fertilizer that the present invention produces:
On wheat, tobacco two big crops, carried out experiment and demonstration according to Fu County, Shaanxi Province agricultural technology center, Fu County drought resisting service team with regard to biological fermentation fulvic acid bacterial manure of the present invention.
One, experiment and demonstration place overview
The wheat point: test arrangement is at dealings town, Fu County, Shaanxi Province Dong Qiezicun, and family Feng Yun is prosperous in test.Experimental field preceding stubble is a Lou soil, and sampling survey soil organic matter content is 1.06% before broadcasting, alkali-hydrolyzable nitrogen 79.3ppm, and rapid available phosphorus 15.0ppm, available potassium 147.3ppm broadcasts preceding in conjunction with 25 kilograms in whole land bulk application urea, 50 kilograms of calcium superphosphate.Adopted the drilling of artificial trench digging on October 7,0.2 meter of line-spacing, ripe back is by sub-district sampling product.Wheat breed is for laying down for a short time No. 22.
Flue-cured tobacco point: two groups of test arrangements, test family Liu two dogs in Niu Wu village, Niu Wu town, Fu County, Shaanxi Province.Experimental field P in soil H value is 8.1, and organic content is 1.3%, alkali-hydrolyzable nitrogen 67.5ppm, rapid available phosphorus 14.1ppm, available potassium 158.3ppm, every mu of 20 kilograms of ammonium nitrate of bulk application, 50 kilograms of calcium superphosphate before broadcasting.
Two, test design
1, wheat:
If four processing are dressed seed, dressed seed+spray, spray and contrast to biochemical fulvic acid.Seed dressing sprays with said preparation 100 gram/mus and converts 50 kilograms in water with 60 times of diluents of said preparation, dials twice of joint period interval spray in 20 days at wheat.Randomized block design is adopted in this test, triplicate, and 7 meters of zone leaders, wide 4 meters, 28 square metres of sub-district areas.
2 flue-cured tobaccos:
Test divides two processing.
(1) biochemical fulvic acid is handled: mu is converted the water 120 kg with 200 grams, in the perpendicular leaf phase of tobacco, rolls into a ball a phase, prosperously respectively sprays once for a long time.
(2) clear water: contrast field piece sprays the equivalent clear water simultaneously.66.7 square metres of sub-district areas. test random alignment, triplicate. field management is identical.
Three, test-results and conclusion
1, wheat
Each treatment process economic characters sees Table 1.As can be seen from the table, using biochemical fulvic acid handles its economic characters and all is better than control group, particularly grain number per spike and thousand seed weight all apparently higher than control group.
From the yield result of each treatment process of table 2, the seed dressing group is than control group volume increase 22.8%, and the group of dressing seed+spray sprays twice than control group volume increase 10.8% than control group volume increase 34.3%, each treatment effect be successively dress seed+spray＞dress seed＞spray＞contrast.
Table 1 wheat is respectively handled the economic characters cartogram
| Treatment process | Plant height (cm) | Spike length (cm) | Mu spike length (cm) | Grain number per spike (grain) | Thousand seed weight (gram) |
| Contrast | 79.0 | 8.6 | 24.3 | 30.3 | 35.3 |
| Seed dressing | 81.0 | 9.5 | 24.1 | 35.1 | 39.5 |
| Dress seed+spray | 82.6 | 10.2 | 25.6 | 35.8 | 39.9 |
| Spray | 81.3 | 9.6 | 24.8 | 33.3 | 39.1 |
Table 2 is respectively handled the output statistics table
| Treatment process | Repeat (folding per mu yield/jin) | On average (jin/mu) | Stimulation ratio (%) | ||
| I | II | III | |||
| Contrast (water spray) | 565.9 | 510.0 | 528.0 | 534.6 | |
| Seed dressing | 732.3 | 622.0 | 615.5 | 656.6 | 22.8 |
| Dress seed+spray | 676.7 | 787.8 | 689.8 | 718.1 | 34.3 |
| Spray | 566.0 | 633.3 | 578.0 | 592.4 | 10.8 |
2, flue-cured tobacco point
As seen from Table 1, the cigarette seedling spray biochemical fulvic acid to the plant height of seedling, root is long and lateral root has obvious facilitation, the experimental plot comparison increases by 7～8cm according to the field plant height, long 6～the 8cm that increases of root, the lateral root number increases by 19, the leaf look becomes dark greenly by yellowish green, and the cigarette seedling of Pei Yuing is that suitable the producing of later high-quality laid a good foundation like this.
Each processing of table 1 is to the influence of tobacco seedling growth
| Handle | Plant height (cm) | Root long (cm) | Lateral root number (cm) | The leaf look | |
| The experimental plot | I | 15 | 11.3 | 45 | Dark green |
| II | 16.4 | 12.1 | 46 | Dark green | |
| III | 16.8 | 11.9 | 50 | Dark green | |
| The contrast field | I | 7 | 5 | 25 | Yellowish green |
| II | 6.8 | 5.8 | 28 | Yellowish green | |
| III | 6.9 | 5.6 | 31 | Yellowish green | |
Each handles table 2 the yield of tobacco contrast table
| Handle | I (jin/mu) | II (jin/mu) | III (jin/mu) | On average (jin/mu) |
| The experimental plot | 206 | 220 | 249 | 225 |
| The contrast field | 180 | 160 | 200 | 180 |
| Volume increase | 26 | 60 | 49 | 45 |
Use biochemical fulvic acid and have significant effect of increasing production on tobacco, the yield increased of every mu of tobacco leaf can increase by 25%, 90 yuan of every mu of net return, and synergy significantly can large scale application.
Utilize the present invention to handle fresh fruit slag and stalk, technology is simple, and selected is sophisticated industrialization suite of equipment, and it is big to handle pomace and stalk amount.The process that under all kinds of microbial processs, generates according to xanthohumic acid bacterial manure, design and be fit to solid, the semisolid zymotechnique that xanthohumic acid bacterial manure is produced, make all kinds of floras can high-quality under the suitable culture condition, fermentative production fulvic acid bacterial manure efficiently.
Environment is not only protected in the production application of this product, has made full use of resource, can also improve the soil and reduce growing of plant pest, improves land quality and utilization ratio, reaches farm crop high-quality, highly efficient and productive purpose.
The invention solves the pollution difficult problem of present fruit juice factory and office reason pomace, utilize waste to produce fulvic acid bacterial manure,, promote the development of the ecological agriculture, have significant environmental benefit, economic benefit and social benefit for enterprise increases considerable profit.
Claims (9)
1. one kind is utilized the fermenting waste from farm and sideline product processing to produce fulvic acid bacterial manure method, and all kinds of waste from farm and sideline product processing are changed into xanthohumic acid through microbial solid one semisolid fermentation, and its processing step is as follows:
(1) aspergillus niger (Aspergillus niger), viride (Trichoderma viride), Bacillus subtillis (Bacillus subtilis), false pseudomonas bacillus (Pseudomonas sp.), distillery yeast (Saccharomyces cerevisiae), candiyeast (Candida sp.) are distinguished successively through test tube cultivation, shake-flask culture, seed tank culture, seed culture fluid is inserted the fermentor tank liquid culture by 10% weight inoculum size, culture temperature is 25～35 ℃, incubation time is 24～72 hours, cultivate into bacteria suspension, its viable count 〉=10
8Individual/ml;
Lactobacillus delbruckii is that the static anaerobism of liquid is cultivated, and progressively classification enlarged culturing, culture temperature are 40～60 ℃, and incubation time is 24～48 hours, its viable count 〉=10
8Individual/ml;
(2) with fermentor tank gained aspergillus niger, viride, Bacillus subtillis, false pseudomonas bacillus bacteria suspension, respectively with 0.5～2: 0.5～2: 0.5～2: 0.5～2 volume ratio inserts the sterilization solid medium, makes after stirring to produce and uses the solid mix bacterium agent;
(3) material pre-treatment: pH value is transferred to 5.5 less than the pH value of 5.5 waste from farm and sideline product processing;
(4) will be 1～5% described production with the weight of material count number inserts pretreated material with the urea of solid mix bacterium agent and 1～2%, stirs and carries out solid fermentation, and 25～45 ℃ of leavening temperatures, fermentation time are 5～10 days;
(5) lactobacillus delbruckii, distillery yeast, candiyeast bacteria suspension, three kinds of bacteria suspensions are by 0.5～2: 0.5～2: 0.5～2 volume ratio is mixed into to produce uses the mixed bacterium suspension;
(6) after the material solid fermentation is finished, tunning is semi-solid state because of there being big quantity of fluid to ooze out, be that 1～5% described production inserts the semisolid fermentation product with the mixed bacterium suspension with tunning weight count number, stir and carry out semisolid fermentation, leavening temperature is 20～40 ℃, and fermentation time is 5～10 days;
(7) reduce to below 1% when the tunning sugar, pH value is reduced to below 3, and viable count reaches 10
8/ ml, fermentation is promptly finished; Tunning after filtration, gained filtrate is the liquid fulvic acid bacterial manure, filter residue is the solid fulvic acid bacterial manure.
2, method according to claim 1, wherein aspergillus niger, viride, Bacillus subtillis, false pseudomonas bacillus bacteria suspension, respectively with 1: 1: 1: 1: 1 volume ratio inserts the sterilization solid medium.
3, method according to claim 1, wherein lactobacillus delbruckii, distillery yeast, candiyeast bacteria suspension, three kinds of bacteria suspensions mix by 1: 1: 1 volume ratio.
4, method according to claim 1, wherein different production bacterial classifications adopts corresponding separately substratum in described step (1), and the culture temperature that test tube is cultivated is 25～35 ℃, and incubation time is 24～72 hours; The culture temperature of shake-flask culture is 25～35 ℃, and rotating speed is 180～220 rev/mins, and incubation time is 24-48 hour; The culture temperature of seed tank culture is 25～35 ℃, and incubation time is 24～48 hours.
5, use potassium primary phosphate (KH in the method according to claim 1, wherein said step (3)
2PO
4) or lime carbonate (CaCO
3) pH value of described material is transferred to 5.5.
6, method according to claim 1, wherein waste from farm and sideline product processing is pomace and stalk.
7, tunning carries out solid-liquid separation by plate filter in the method according to claim 1, wherein said step (7).
8, method according to claim 1, wherein said filter residue is through drying or drying, and moisture content is less than 13%.
9, method according to claim 8 wherein is pressed into desired shape, size particles with single-lead-screw extruding briquetting machine with filter residue, through weighing, packing, becomes the solid fulvic acid bacterial manure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101620416A CN100497267C (en) | 2006-12-11 | 2006-12-11 | Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101620416A CN100497267C (en) | 2006-12-11 | 2006-12-11 | Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure |
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| Publication Number | Publication Date |
|---|---|
| CN1974490A true CN1974490A (en) | 2007-06-06 |
| CN100497267C CN100497267C (en) | 2009-06-10 |
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| CN101774853A (en) * | 2010-01-07 | 2010-07-14 | 杨雪枣 | Antibacterial type composite biological organic liquid fertilizer and preparation method thereof |
| CN101401589B (en) * | 2008-11-19 | 2011-04-20 | 东北农业大学 | Method of preparing microbial source stress resistance |
| CN101665375B (en) * | 2009-09-04 | 2011-06-15 | 上海交通大学 | Preparation method of straw bacterial manure |
| CN102206102A (en) * | 2011-03-11 | 2011-10-05 | 四川省兰月农化科技开发有限责任公司 | Production method for fulvic acid with rice straws |
| CN101439996B (en) * | 2008-12-18 | 2012-01-25 | 杨雪枣 | Flower-protecting and sweet-enhancing composite biological-organic liquid fertilizer |
| CN101602623B (en) * | 2009-07-14 | 2012-07-11 | 武汉诚亿生物制品有限公司 | Microbial organic fertilizer and compound fertilizer, and preparation methods thereof |
| CN103467144A (en) * | 2012-06-06 | 2013-12-25 | 福建省诏安县绿洲生化有限公司 | Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof |
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| CN109355346A (en) * | 2018-11-26 | 2019-02-19 | 萍乡市乐乐腐植酸厂 | A kind of method of apple pomace semisolid fermentation production biochemical fulvic acid |
| CN110668880A (en) * | 2019-11-10 | 2020-01-10 | 砀山海升果胶有限责任公司 | Method for producing carrot liquid organic fertilizer from pomace after apple pectin extraction |
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| CN101401589B (en) * | 2008-11-19 | 2011-04-20 | 东北农业大学 | Method of preparing microbial source stress resistance |
| CN101439996B (en) * | 2008-12-18 | 2012-01-25 | 杨雪枣 | Flower-protecting and sweet-enhancing composite biological-organic liquid fertilizer |
| CN101602623B (en) * | 2009-07-14 | 2012-07-11 | 武汉诚亿生物制品有限公司 | Microbial organic fertilizer and compound fertilizer, and preparation methods thereof |
| CN101665375B (en) * | 2009-09-04 | 2011-06-15 | 上海交通大学 | Preparation method of straw bacterial manure |
| CN101774853A (en) * | 2010-01-07 | 2010-07-14 | 杨雪枣 | Antibacterial type composite biological organic liquid fertilizer and preparation method thereof |
| CN101774853B (en) * | 2010-01-07 | 2014-04-02 | 杨雪枣 | Antibacterial type composite biological organic liquid fertilizer and preparation method thereof |
| CN102206102B (en) * | 2011-03-11 | 2012-12-05 | 四川省兰月农化科技开发有限责任公司 | Production method for fulvic acid with rice straws |
| CN102206102A (en) * | 2011-03-11 | 2011-10-05 | 四川省兰月农化科技开发有限责任公司 | Production method for fulvic acid with rice straws |
| CN103467144A (en) * | 2012-06-06 | 2013-12-25 | 福建省诏安县绿洲生化有限公司 | Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof |
| CN103467144B (en) * | 2012-06-06 | 2015-01-07 | 福建省诏安县绿洲生化有限公司 | Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof |
| CN104069042A (en) * | 2014-07-23 | 2014-10-01 | 李雪萍 | Biochemical fulvic acid (BFA) cosmetic preparation |
| CN109355346A (en) * | 2018-11-26 | 2019-02-19 | 萍乡市乐乐腐植酸厂 | A kind of method of apple pomace semisolid fermentation production biochemical fulvic acid |
| CN109355346B (en) * | 2018-11-26 | 2022-08-09 | 萍乡市乐乐腐植酸厂 | Method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace |
| CN110668880A (en) * | 2019-11-10 | 2020-01-10 | 砀山海升果胶有限责任公司 | Method for producing carrot liquid organic fertilizer from pomace after apple pectin extraction |
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| CN100497267C (en) | 2009-06-10 |
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Assignee: Xi'an Rui Rui Biotechnology Co., Ltd. Assignor: Chen Wuling Contract fulfillment period: 2009.12.1 to 2019.11.30 Contract record no.: 2009610000145 Denomination of invention: Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure Granted publication date: 20090610 License type: Exclusive license Record date: 2009.12.9 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.12.1 TO 2019.11.30; CHANGE OF CONTRACT Name of requester: XI'AN ZIRUI BIOTECHNOLOGY CO., LTD. Effective date: 20091209 |
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Effective date of registration: 20190701 Address after: 710077 No. 56 Jinye Road, Xi'an Hi-tech District, Shaanxi Province, 2129, 21 Floor, Block B, Xi'an Yanxiang City Square Patentee after: Xi'an Rui Rui Biotechnology Co., Ltd. Address before: 710069 No. 202, 3A Building, Taoyuan Campus, Northwest University, High-tech Fourth Road, Lianhu District, Xi'an City, Shaanxi Province Patentee before: Chen Wuling |