CN100497267C - Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure - Google Patents
Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure Download PDFInfo
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- CN100497267C CN100497267C CNB2006101620416A CN200610162041A CN100497267C CN 100497267 C CN100497267 C CN 100497267C CN B2006101620416 A CNB2006101620416 A CN B2006101620416A CN 200610162041 A CN200610162041 A CN 200610162041A CN 100497267 C CN100497267 C CN 100497267C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
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Abstract
The present invention relates to production of fulvic acid, and is especially the process of producing fulvic acid bacterial manure with waste from farm and sideline product processing and through fermentation and conversion. Through selecting and breeding bacteria strain, proper solid-semi-solid fermentation process, and re-compounding fulvic acid and nitrogen fertilizer, phosphate fertilizer and potash fertilizer bacteria, the present invention produces the fulvic acid bacterial manure with excellent synergistic effects of regulating the opening of foliage air pore, reducing water evaporation, retaining water, promoting root system growth and raising the drought fighting capacity of crop.
Description
Technical field
The present invention relates to a kind of agricultural waste of utilizing and make the method for bacterial manure through microbial fermentation, particularly a kind of pomace, all kinds of agricultural crop straw of utilizing changes into the production technique of fulvic acid bacterial manure after many bacterial classifications of microorganism solid-semi-solid mixed fermentation.
Background technology
" pomace " among the present invention be meant all kinds of fruit fruit juice source mill squeeze the juice back or a kind of watermelon get seed after the general designation of the waste residue that produces.The pomace gross protein value is lower, the only about 1-2% of weight percent, but sugar and organic acid content are higher.In addition, also contain major elements such as more rich calcium, phosphorus, potassium, iron, manganese, sulphur and trace element etc.Along with the emergence of China's fruit juice processing industry, the pomace generation increases year by year in recent years, is used as the feed except a small amount of, and these residues mainly go out of use, and form new industrial or agricultural rubbish, and new problem and burden are brought to environmental protection in the back of rotting.
Biochemical fulvic acid (BFA) is a method of utilizing biotechnology, is raw material with agricultural wastes, the product that the decomposition of the biology that process is complicated under the microbial fermentation effect, chemistry and building-up reactions generate.The biochemical fulvic acid as fertilizer sources uses, and compares with the mineral resources humic acid, and it has following characteristics of three aspects:
1, molecular weight is little, is utilized by plant absorbing easily;
2, functional group content is many, the physiologically active height;
3, can be directly water-soluble, it is acid that its aqueous solution is.
This of biochemical fulvic acid three big features the agricultural, pharmaceutically practical application effect is more superior than the mineral resources humic acid.
Biochemical fulvic acid (BFA) is a microbial fermentation and generating, it is the very complicated mixture of a kind of composition, contain in a large number biologically active substance and nutritions such as the influential enzyme of growing process, amino acid, VITAMIN and trace elements, to promoting plant-growth, growing remarkable efficacy is arranged.BFA has good water-solubility, in soil, can strengthen the surge capability of soil by complexing, chelating, adsorption, improve guarantor's fertilizer, the water retention capacity of soil, improve the ventilation property of soil and the activity of soil microorganisms, increase effective flora quantity and reach the effect of improving the soil.BFA is the material of finding at present of regulating blade face pore opening degree most effectively, it can dwindle blade face pore opening degree, reduce moisture transpiration, make plant and Soil conservation more water, promote the root system development growth simultaneously, improve the root system vigor and make crop absorb more water and nutriment that the two complements each other, increase income and decrease expenditure, reach the purpose that improves crop drought resistance water saving ability.BFA is a kind of good plant-growth regulator; BFA has stronger ionic bond ability, can significantly improve plant to trace elements absorbed and running ability.Composite with chemical fertilizer, can obviously increase fertilizer efficiency, reduce fertilizer amount, reduce loss of chemical fertilizer.
At present, produce xanthohumic acid both at home and abroad and mainly contain following method:
1, utilize coal (peat, brown coal, weathered coal) to produce xanthohumic acid.
2, chemosynthesis xanthohumic acid.
3, biological fermentation is produced xanthohumic acid.
First kind of method of utilizing coal (peat, brown coal, weathered coal) to produce xanthohumic acid, need coal to be degraded with chemical process, in its degradation process, need to consume chemical substances such as a large amount of acid, alkali, what have also needs violent condition such as high temperature, high pressure and strict process and specific installation, consume lot of energy, produce waste water, waste gas simultaneously, environment is polluted.
External many people adopt the method for second kind of chemosynthesis xanthohumic acid, the generative process of simulation natural humus acid, utilize polyphenol, quinone, carbohydrate, urea, xylogen or itrogenous organic substance etc. to carry out the synthetic xanthohumic acid of oxidative condensation, cost is high and be difficult to scale operation.
Utilize agricultural wastes such as pomace, stalk, produce biochemical fulvic acid by microbial fermentation, can make refuse obtain utilizing, can not pollute environment, raw material is easy to get, and cost is low.
Summary of the invention
The purpose of this invention is to provide a kind of agricultural waste such as pomace, stalk of utilizing, adopt many bacterial classifications of microorganism solid-semi-solid mixed fermentation technology to produce the production technique of fulvic acid bacterial manure, both solve the problem of environmental pollution that a large amount of pomaces are rotted, crop straw burning brings, again it had been become the biological organic fungi-manure of super quality and competitive price.
The present invention realizes by the following technical solutions:
1, the present invention is finished by following steps,
(a), with aspergillus niger (Aspergillus niger), viride (Trichodermaviride), subtilis (Bacillus subtilis), false monospore bacillus (Pseudomonas sp.), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), candiyeast (Candida sp.) is cultivated through test tube respectively successively, shake-flask culture, seed tank culture, seed tank culture liquid is inserted the fermentor tank liquid culture respectively by 10% weight inoculum size, culture temperature is 25-35 ℃, incubation time is 24-72 hour, cultivate into production high density thalline bacteria suspension, viable count 〉=10
8Individual/ml;
(b), lactobacillus delbruckii (Lactobacillus delbrueckii) is that the static anaerobism of liquid is cultivated, progressively classification enlarged culturing, culture temperature are 40-60 ℃, incubation time is 24-48 hour, its viable count 〉=10
8Individual/ml;
(c), with fermentor tank gained aspergillus niger, viride, subtilis, false monospore bacillus high-concentration bacterial suspension, volume ratio with 0.5-2:0.5-2:0.5-2:0.5-2 inserts the sterilization solid medium respectively respectively, make after stirring and produce that to use solid mix bacterium agent, viable count be 〉=10
8Individual/g, described solid medium can be a bran mass;
Aspergillus niger, viride, subtilis, false monospore bacillus thalline bacteria suspension preferably insert the sterilization solid medium with the volume ratio of 1:1:1:1;
(d), material pre-treatment: owing to material variety and fruit picking phase difference, the pH value of material distributes very big, the apple pomace pH value 5.5 in ripening stage, immature apple pomace pH value 3.5-4, the potassium primary phosphate (KH of elder generation before the inoculation
2PO
4) or lime carbonate (CaCO
3), pH value is transferred to pH value 5.5 less than 5.5 material; Need not to regulate pH value for pH value greater than 5.5 material;
(e), will insert pretreated material with the urea of solid mix bacterium agent and 1-2% with the production of weight of material 1-5%, stir and carry out solid fermentation, leavening temperature 25-45 ℃, fermentation time is 5-10 days, viable count is 〉=10
8Individual/g;
(f), lactobacillus delbruckii, yeast saccharomyces cerevisiae, candiyeast bacteria suspension, three kinds of bacteria suspensions are mixed into to produce by the volume ratio of 0.5-2:0.5-2:0.5-2 uses the mixed bacterium suspension; Preferably the volume ratio with 1:1:1 mixes;
(g), after the material solid fermentation is finished, tunning is semi-solid state because of there being big quantity of fluid to ooze out, with tunning weight count number is the described production mixed bacterium suspension access semisolid fermentation product of 1-5%, stir and carry out semisolid fermentation, leavening temperature is 20-40 ℃, and fermentation time is 5-10 days;
(h), reduce to below 1%, pH value is reduced to below 3 when the tunning sugar, and viable count reaches 〉=and 10
8/ ml, the fermentation promptly finish, tunning after filtration, gained filtrate is the liquid fulvic acid bacterial manure, filter residue is the solid fulvic acid bacterial manure; Filter residue is through drying or drying, and moisture content is squeezed into desired shape, size particles less than 13% with single-lead-screw extruding briquetting machine, through weighing, packing, promptly becomes solid fulvic acid bacterial manure product.
The used bacterial classification of the present invention all is known bacterial classification, can obtain by known screening, commercial means or other approach, candiyeast and false monospore bacillus all are bacterial classifications commonly used in this area, as candiyeast can be candida tropicalis (Candida tropicalis), and false monospore bacillus can be pseudomonas fluorescens (Pseudomonas fluorescens).
Each used substratum all is the conventional substratum of the described microorganism of known suitable cultivation in the cultural methods such as test tube cultivation of the present invention, shake-flask culture, seed tank culture, fermentor cultivation, enlarged culturing.
Above treatment process both had been applicable to fresh fruit slag without any processing, fresh straw, also had been applicable to pomace, stalk after handling with the fresh fruit slag oven dry or with additive method.
The invention has the advantages that:
1, adopts modern advanced strain improvement technology, selected and efficiently to have transformed all kinds of production bacterial classifications that pomace, stalk become biochemical fulvic acid, and highly active vinelandii, phosphorus bacteria, potassium bacterium, guaranteed to produce the high quality of bacterial classification.
2, the process that generates under all kinds of microbial processs according to xanthohumic acid is designed and is fit to solid-semisolid zymotechnique that xanthohumic acid is produced, make all kinds of floras can high-quality under the suitable culture condition, ferment efficiently and produce xanthohumic acid.
3, adopt advanced deep layer fermenting process, solved mixed fermentation nitrogen. phosphorus. a technology of potassium bacterium and a prescription difficult problem, both saved equipment, can obtain effective viable bacteria body of lot of pure again, (the effective viable bacteria body of fermented liquid can reach 10
10Individual/ml), be that three kinds of bacterium synergy raising fertilizer efficiency lay the foundation.
4, worked out biochemical fulvic acid and nitrogen. phosphorus. the compounded formula of potassium bacterium, it is composite to carry out science at the special-purpose fertilizer of different sorts crop, two biolobic material fertilizer can be organically combined, collaborative performance effect.Analogous products have been yet there are no in the fertilizer industry at present.
5, owing to the inexpensive raw material of employing, good production bacterial classification, advanced technology, scientific formula, this project various product is compared production cost with similar other products and will be descended significantly, and action effect is then very remarkable.
6, the xanthohumic acid bacterial manure that obtains of the present invention can be regulated the material of blade face pore opening degree effectively, can play and dwindle blade face pore opening degree, reduce moisture transpiration, make plant and Soil conservation more water, promote the root system development growth simultaneously, reach the purpose that improves crop drought resistance water saving ability.
Embodiment
Following embodiment is to further explanation of the present invention and explanation, and the present invention is not constituted any limitation.
Example I:
20 tons of fermentative processing fresh apple slags, its main component is: carbohydrate about 20%, robust fibre 10-15%, water content 45%-55%, crude protein 5.6%, crude fat 6.2%, calcium 0.06%, phosphorus 0.05%, trace copper 11.8mg/kg, zinc 15.4mg/kg, manganese 14.0mg/kg, iron 158mg/kg, selenium 0.08mg/kg, aminoacids content 0.5%; PH value 4.5.
The preparation method:
(1), aspergillus niger, viride, subtilis, pseudomonas fluorescens, yeast saccharomyces cerevisiae and candida tropicalis are cultivated through test tube respectively, well-known different production bacterial classifications adopts corresponding separately substratum, culture temperature is 25-35 ℃, and incubation time is 24-72 hour; Bacterial classification changed shake-flask culture over to after test tube was cultivated and finished, and culture temperature is 25-35 ℃, and rotating speed is 180-220 rev/min, and incubation time is 24-48 hour; Bacterial classification inserted the seeding tank stir culture after shake-flask culture was finished, and culture temperature is 25-35 ℃, and incubation time is 24-48 hour; After seed tank culture is finished, insert the fermentor tank liquid culture by 10% weight inoculum size, culture temperature is 28-32 ℃, incubation time 36-42 hour; Aspergillus niger, viride, subtilis, pseudomonas fluorescens are cultivated gained high-concentration bacterial suspension insert the sterilization bran mass in the ratio of 1:1:1:1 respectively, make to produce and use the solid mix bacterium agent; Yeast saccharomyces cerevisiae and candida tropicalis are used in the bacteria suspension mode;
(2), lactobacillus delbruckii adopts the static culture method of liquid, progressively classification enlarged culturing, culture temperature are 50-54 ℃, each incubation time is 34-40 hour;
(3), utilize potassium primary phosphate (KH
2PO
4) pomace is transferred to pH value 5.5;
(4), will be that the urea of the described solid mix bacterium agent of 2-4% and 1-2% and modulated pomace stir and carry out solid fermentation with pomace weight count number, leavening temperature is 30-40 ℃, fermentation time 6-8 days;
(5), lactobacillus delbruckii, yeast saccharomyces cerevisiae, candida tropicalis bacteria suspension, three kinds of bacteria suspensions become the mixed bacterium suspension by the 1:1:1 mixed;
(6), after the pomace solid fermentation is finished, in the tunning because of there being big quantity of fluid to ooze out, be semi-solid state, to be the above-mentioned production mixed bacterium suspension access semisolid fermentation product of 1-5% with tunning weight count number this moment, stir and carry out semisolid fermentation, leavening temperature is 20-40 ℃, and fermentation time is 5-10 days;
(7), reduce to below 1%, pH value is reduced to below 3, and viable count is 〉=10 when the fermented liquid sugar
8Fermentation is finished during/ml.Tunning is carried out solid-liquid separation by plate filter, and gained filtrate is the liquid fulvic acid bacterial manure, and filter residue can be used as the solid fulvic acid bacterial manure.Filter residue is through drying or drying, and moisture is reduced to about 13%, is squeezed into desired shape, size particles with single-lead-screw extruding briquetting machine, through weighing, packing, promptly becomes solid fulvic acid bacterial manure product.
Liquid fulvic acid bacterial manure and solid fulvic acid bacterial manure main component are:
Liquid fulvic acid bacterial manure main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Viable count 〉=10
8/ ml.
Solid fulvic acid bacterial manure main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Organic 〉=40%; Viable count 〉=10
9/ g; Shui Fen ≦ 13%; Granularity
2.5～4.5mm 〉=80%.
Example II:
20 tons of fermentative processing sweet sorghum straws, its main component is: crude protein 8.2% in the sweet sorghum seed, crude fat 2.6%, robust fibre 3.6%, ash content 3.9%, calcium 0.07%, phosphorus 0.24%; Juice containing amount is 70-80% in the cane, and it contains sugar hammer degree Bri is 17-21%, pH value 7.0.Be crushed to 〉=80% material particles diameter is 2.5-4.5mm.
The preparation method:
Aspergillus niger, viride, subtilis, pseudomonas fluorescens, yeast saccharomyces cerevisiae and candida tropicalis are cultivated through test tube respectively, well-known different production bacterial classifications adopts corresponding separately substratum, the test tube culture temperature is 25-35 ℃, incubation time 24-72 hour; Bacterial classification changed over to and shakes bottle after test tube was cultivated and finished, and the shake-flask culture temperature is 25-35 ℃, and rotating speed is 180-220 rev/min, incubation time 24-48 hour; Bacterial classification inserted the seeding tank stir culture after shake-flask culture was finished, and the seed tank culture temperature is 25-35 ℃, incubation time 24-48 hour; Insert the fermentor tank liquid culture by 10% weight inoculum size, culture temperature is 25-35 ℃, incubation time 24-72 hour; Aspergillus niger, viride, subtilis, pseudomonas fluorescens are cultivated gained high-concentration bacterial suspension insert the sterilization bran mass in the ratio of 1:1:1:1 respectively, make to produce and use the solid mix bacterium agent;
(2), lactobacillus delbruckii adopts progressively classification enlarged culturing of the static culture method of liquid, culture temperature is 50-54 ℃, incubation time is 34-40 hour;
(3), will be that the described production of 2-4% stirs with the urea of solid mix bacterium agent and 1-2% and modulated stalk and carries out solid fermentation with stalk weight count number, leavening temperature is 30-45 ℃, fermentation time 6-10 days;
(4), lactobacillus delbruckii, yeast saccharomyces cerevisiae, candiyeast bacteria suspension, three kinds of bacteria suspensions become the mixed bacterium suspension by the 1:1:1 mixed;
(5), after the stalk solid fermentation is finished, there is big quantity of fluid to ooze out in the tunning, be semi-solid state, to be the above-mentioned production mixed bacterium suspension access semisolid fermentation product of 1-5% with tunning weight count number this moment, stir and carry out semisolid fermentation, leavening temperature is 20-40 ℃, and fermentation time is 5-10 days;
(6), the fermented liquid sugar reduces to below 1%, pH value is reduced to below 3, viable count is 〉=10
8Fermentation is finished during/ml.Tunning is carried out solid-liquid separation by plate filter, and gained filtrate is the liquid fulvic acid bacterial manure, and filter residue can be used as the solid fulvic acid bacterial manure.Filter residue is through drying or drying, and moisture content should be squeezed into desired shape, size particles with single-lead-screw extruding briquetting machine less than 13%, through weighing, packing, promptly becomes the solid fulvic acid bacterial manure.
Liquid and solid xanthohumic acid main component are:
Liquid xanthohumic acid main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Viable count 〉=10
8/ ml.
Solid xanthohumic acid main component: biochemical fulvic acid 〉=8%; Trace element 〉=3%; Aminoacids content 〉=1.5%; Viable count 〉=10
9/ g; Organic 〉=40%; Moisture≤13%; Granularity
2.5～4.5mm 〉=80%.
The application test of the xanthohumic acid fertilizer that the present invention produces:
On wheat, tobacco two big crops, carried out experiment and demonstration according to Fu County, Shaanxi Province agricultural technology center, Fu County drought resisting service team with regard to biological fermentation fulvic acid bacterial manure of the present invention.
One, experiment and demonstration place overview
The wheat point: test arrangement is at dealings town, Fu County, Shaanxi Province Dong Qiezicun, and family Feng Yun is prosperous in test.Experimental field preceding stubble is a Lou soil, and sampling survey soil organic matter content is 1.06% before broadcasting, alkali-hydrolyzable nitrogen 79.3p pm, and rapid available phosphorus 15.0ppm, available potassium 147.3ppm broadcasts preceding in conjunction with 25 kilograms in whole land bulk application urea, 50 kilograms of calcium superphosphate.Adopted the drilling of artificial trench digging on October 7,0.2 meter of line-spacing, ripe back is by sub-district sampling product.Wheat breed is for laying down for a short time No. 22.
Flue-cured tobacco point: two groups of test arrangements, test family Liu two dogs in Niu Wu village, Niu Wu town, Fu County, Shaanxi Province.Experimental field P in soil H value is 8.1, and organic content is 1.3%, alkali-hydrolyzable nitrogen 67.5ppm, rapid available phosphorus 14.1ppm, available potassium 158.3ppm, every mu of 20 kilograms of ammonium nitrate of bulk application, 50 kilograms of calcium superphosphate before broadcasting.
Two, test design
1, wheat:
If four processing are dressed seed, dressed seed+spray, spray and contrast to biochemical fulvic acid.Seed dressing sprays with said preparation 100 gram/mus and converts 50 kilograms in water with 60 times of diluents of said preparation, dials twice of joint period interval spray in 20 days at wheat.Randomized block design is adopted in this test, triplicate, and 7 meters of zone leaders, wide 4 meters, 28 square metres of sub-district areas.
2 flue-cured tobaccos:
Test divides two processing.
(1) biochemical fulvic acid is handled: mu is converted the water 120 kg with 200 grams, in the perpendicular leaf phase of tobacco, rolls into a ball a phase, prosperously respectively sprays once for a long time.
(2) clear water: contrast field piece sprays the equivalent clear water simultaneously.66.7 square metres of sub-district areas. test random alignment, triplicate. field management is identical.
Three, test-results and conclusion
1, wheat
Each treatment process economic characters sees Table 1.As can be seen from the table, using biochemical fulvic acid handles its economic characters and all is better than control group, particularly grain number per spike and thousand seed weight all apparently higher than control group.
From the yield result of each treatment process of table 2, the seed dressing group is than control group volume increase 22.8%, and the group of dressing seed+spray sprays twice than control group volume increase 10.8% than control group volume increase 34.3%, and each treatment effect is to dress seed+spray successively〉seed dressing〉spray contrast.
Table 1 wheat is respectively handled the economic characters cartogram
| Treatment process | Plant height (cm) | Spike length (cm) | Mu spike length (cm) | Grain number per spike (grain) | Thousand seed weight (gram) |
| Contrast | 79.0 | 8.6 | 24.3 | 30.3 | 35.3 |
| Seed dressing | 81.0 | 9.5 | 24.1 | 35.1 | 39.5 |
| Dress seed+spray | 82.6 | 10.2 | 25.6 | 35.8 | 39.9 |
| Spray | 81.3 | 9.6 | 24.8 | 33.3 | 39.1 |
Table 2 is respectively handled the output statistics table
2, flue-cured tobacco point
As seen from Table 1, the cigarette seedling spray biochemical fulvic acid to the plant height of seedling, root is long and lateral root has obvious facilitation, the experimental plot comparison increases 7-8cm according to the field plant height, the long 6-8cm that increases of root, the lateral root number increases by 19, the leaf look becomes dark greenly by yellowish green, and the cigarette seedling of Pei Yuing is that suitable the producing of later high-quality laid a good foundation like this.
Each processing of table 1 is to the influence of tobacco seedling growth
Each handles table 2 the yield of tobacco contrast table
| Handle | I (jin/mu) | II (jin/mu) | III (jin/mu) | On average (jin/mu) |
| The experimental plot | 206 | 220 | 249 | 225 |
| The contrast field | 180 | 160 | 200 | 180 |
| Increase) | 26 | 60 | 49 | 45 |
Use biochemical fulvic acid and have significant effect of increasing production on tobacco, the yield increased of every mu of tobacco leaf can increase by 25%, 90 yuan of every mu of net return, and synergy significantly can large scale application.
Utilize the present invention to handle agricultural waste such as fresh fruit slag, granulated slag, all kinds of agricultural crop straw, excrement of animals, technology is simple, and selected is sophisticated industrialization suite of equipment, and it is big to handle agricultural waste material amount.The process that under all kinds of microbial processs, generates according to xanthohumic acid bacterial manure, design and be fit to solid, the semisolid zymotechnique that xanthohumic acid bacterial manure is produced, make all kinds of floras can high-quality under the suitable culture condition, ferment efficiently and produce fulvic acid bacterial manure.
The production application of this product not only helps reduce polluting, and the protection environment has made full use of resource, can also improve the soil and reduce growing of plant pest, improves land quality and utilization ratio, reaches to impel crop growth and high-quality, highly efficient and productive purpose.
The invention solves the pollution difficult problem of agricultural waste such as present processing pomace, all kinds of agricultural crop straws, utilize agricultural waste to produce fulvic acid bacterial manure,, promote the development of the ecological agriculture for enterprise increases considerable profit, has significant environmental benefit, economic benefit and social benefit.
Claims (9)
1. the method that pomace, stalk fermentation are produced fulvic acid bacterial manure is characterized in that pomace, stalk are changed into fulvic acid bacterial manure through microbial solid-semisolid fermentation, finish by following steps,
(a), with aspergillus niger (Aspergillus niger), viride (Trichodermaviride), subtilis (Bacillus subtilis), false monospore bacillus (Pseudomonassp.), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), candiyeast (Candida sp.) is cultivated through test tube respectively successively, shake-flask culture, seed tank culture, seed tank culture liquid is inserted the fermentor tank liquid culture respectively by 10% weight inoculum size, culture temperature is 25-35 ℃, incubation time is 24-72 hour, cultivate into production high density thalline bacteria suspension, viable count 〉=10
8Individual/ml;
(b), lactobacillus delbruckii (Lactobacillus delbrueckii) is that the static anaerobism of liquid is cultivated, progressively classification enlarged culturing, culture temperature are 40-60 ℃, incubation time is 24-48 hour, its viable count 〉=10
8Individual/ml;
(c), with fermentor tank gained aspergillus niger, viride, subtilis, false monospore bacillus high density thalline bacteria suspension, volume ratio with 0.5-2:0.5-2:0.5-2:0.5-2 inserts the sterilization solid medium respectively respectively, makes after stirring to produce and uses the solid mix bacterium agent;
(d), material pre-treatment: earlier pH value is transferred to pH value 5.5 less than 5.5 material before the inoculation;
(e), will insert pretreated material with the urea of solid mix bacterium agent and 1-2% with the production of weight of material 1-5%, stir and carry out solid fermentation, leavening temperature 25-45 ℃, fermentation time is 5-10 days, viable count is 〉=10
8Individual/g;
(f), with lactobacillus delbruckii, yeast saccharomyces cerevisiae, candiyeast bacteria suspension, three kinds of bacteria suspensions are mixed into to produce by the volume ratio of 0.5-2:0.5-2:0.5-2 uses the mixed bacterium suspension;
(g), after the material solid fermentation is finished, tunning is semi-solid state because of there being big quantity of fluid to ooze out, with tunning weight count number is the described production mixed bacterium suspension access semisolid fermentation product of 1-5%, stir and carry out semisolid fermentation, leavening temperature is 20-40 ℃, and fermentation time is 5-10 days;
(h), reduce to below 1%, pH value is reduced to below 3 when the tunning sugar, and viable count reaches 〉=and 10
8/ ml, the fermentation promptly finish, tunning after filtration, gained filtrate is the liquid fulvic acid bacterial manure, filter residue is the solid fulvic acid bacterial manure.
2, the method for pomace according to claim 1, stalk fermentation production fulvic acid bacterial manure, it is characterized in that in the described step (c), aspergillus niger, viride, subtilis, false monospore bacillus bacteria suspension are with the volume ratio access sterilization solid medium of 1:1:1:1.
3. the method that pomace according to claim 1, stalk fermentation are produced fulvic acid bacterial manure, it is characterized in that in the described step (f), lactobacillus delbruckii, yeast saccharomyces cerevisiae, candiyeast bacteria suspension, three kinds of bacteria suspensions are mixed into to produce with the volume ratio of 1:1:1 uses the mixed bacterium suspension.
4. the method that pomace according to claim 1, stalk fermentation are produced fulvic acid bacterial manure, it is characterized in that in the described step (a), different production bacterial classifications adopts corresponding separately substratum, and the culture temperature that test tube is cultivated is 25～35 ℃, and incubation time is 24～72 hours; The culture temperature of shake-flask culture is 25～35 ℃, and rotating speed is 180～220 rev/mins, and incubation time is 24-48 hour; The culture temperature of seed tank culture is 25～35 ℃, and incubation time is 24～48 hours.
5, the method for pomace according to claim 1, stalk fermentation production fulvic acid bacterial manure is characterized in that in the described step (d), uses earlier potassium primary phosphate KH before the inoculation
2PO
4Or lime carbonate CaCO
3, pH value is transferred to pH value 5.5 less than 5.5 material; Need not to regulate pH value for pH value greater than 5.5 material.
6, the method for pomace according to claim 1, stalk fermentation production fulvic acid bacterial manure is characterized in that material is meant pomace and stalk in the described step (d).
7, the method for pomace according to claim 1, stalk fermentation production fulvic acid bacterial manure is characterized in that in the described step (h) tunning being carried out solid-liquid separation by plate filter.
8. the method that pomace according to claim 1, stalk fermentation are produced fulvic acid bacterial manure is characterized in that: in the described step (h), filter residue is through drying or drying, and moisture content is less than 13%.
9. the method that pomace according to claim 1, stalk fermentation are produced fulvic acid bacterial manure, it is characterized in that: in the described step (h), with single-lead-screw extruding briquetting machine filter residue is squeezed into desired shape, size particles,, promptly becomes solid fulvic acid bacterial manure product through weighing, packing.
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| CN101401589B (en) * | 2008-11-19 | 2011-04-20 | 东北农业大学 | Method of preparing microbial source stress resistance |
| CN101439996B (en) * | 2008-12-18 | 2012-01-25 | 杨雪枣 | Flower-protecting and sweet-enhancing composite biological-organic liquid fertilizer |
| CN101602623B (en) * | 2009-07-14 | 2012-07-11 | 武汉诚亿生物制品有限公司 | Microbial organic fertilizer and compound fertilizer, and preparation methods thereof |
| CN101665375B (en) * | 2009-09-04 | 2011-06-15 | 上海交通大学 | Preparation method of straw bacterial manure |
| CN101774853B (en) * | 2010-01-07 | 2014-04-02 | 杨雪枣 | Antibacterial type composite biological organic liquid fertilizer and preparation method thereof |
| CN102206102B (en) * | 2011-03-11 | 2012-12-05 | 四川省兰月农化科技开发有限责任公司 | Production method for fulvic acid with rice straws |
| CN103467144B (en) * | 2012-06-06 | 2015-01-07 | 福建省诏安县绿洲生化有限公司 | Refined fulvic-acid-containing agricultural microbial inoculum and production method thereof |
| CN104069042A (en) * | 2014-07-23 | 2014-10-01 | 李雪萍 | Biochemical fulvic acid (BFA) cosmetic preparation |
| CN109355346B (en) * | 2018-11-26 | 2022-08-09 | 萍乡市乐乐腐植酸厂 | Method for producing biochemical fulvic acid by semi-solid fermentation of apple pomace |
| CN110668880A (en) * | 2019-11-10 | 2020-01-10 | 砀山海升果胶有限责任公司 | Method for producing carrot liquid organic fertilizer from pomace after apple pectin extraction |
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Assignee: Xi'an Rui Rui Biotechnology Co., Ltd. Assignor: Chen Wuling Contract fulfillment period: 2009.12.1 to 2019.11.30 Contract record no.: 2009610000145 Denomination of invention: Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure Granted publication date: 20090610 License type: Exclusive license Record date: 2009.12.9 |
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