CN105294284B - A kind of preparation method of stalk fermentation bio-feritlizer - Google Patents

A kind of preparation method of stalk fermentation bio-feritlizer Download PDF

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CN105294284B
CN105294284B CN201510805253.0A CN201510805253A CN105294284B CN 105294284 B CN105294284 B CN 105294284B CN 201510805253 A CN201510805253 A CN 201510805253A CN 105294284 B CN105294284 B CN 105294284B
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liquid
fermentation
stalk
decomposed
feritlizer
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CN105294284A (en
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赵红卫
香宾
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河北中仓化肥有限公司
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a kind of preparation method of stalk fermentation bio-feritlizer, it is to smash wheat stalk, maize straw and reed straw, adds water mixing;It is inoculated with decomposed Mixed Microbes, compost fermentation;Sugar residue, sodium humate and poly-aspartate, mixing are sequentially added, sprinkling plant ash aqueous solution adjusts pH value, enzymolysis;Trace element fertilizer and EDTA-2Na stabilizers are added, is uniformly mixed, compound adjusting bacterium is added, packs secondary fermentation;By the low-temperature material drying after fermentation, by the material granulation after drying up to product.The present invention makes full use of stalk resource, a large amount of stalk resources can not only be realized Efficient Conversion, turn waste into wealth, but also the alkalinity in salt-soda soil can be improved, increase the activity of soil, the resistance for improving crop makes the crop that salt-soda soil is planted effectively be increased production, and has very high economic benefit and social benefit.

Description

A kind of preparation method of stalk fermentation bio-feritlizer
Technical field
The present invention relates to plant fermentation organic fertilizer field, specifically a kind of preparation side of stalk fermentation bio-feritlizer Method.
Background technology
China is large agricultural country, and the development of agricultural production be unable to do without fertilizer, and as people chase after planting industry high yield It asks, demand of the China to fertilizer is growing day by day.It is well known that the fertilizer applied in China's agricultural production at present is predominantly changed Fertilizer, chemical fertilizer have many advantages, such as production be easy to scale, transport and apply convenient, efficient raising crop yield, but for a long time with Come the unreasonable soil hardening brought using chemical fertilizer, soil fertility decline, ecological disruption, environmental pollution and agricultural and sideline product quality Decline problem has been subjected to the great attention of national departments concerned and agricultural science and technology circle.
Soil salinization problem has been subjected to the great attention of people, and soil salinization phenomenon mainly appears on more arid Area and continent depression, one band of North China of China Cangzhou there is large stretch of salt-soda soil, plant growth is slow, grain Yield is very low.Method currently used for improveing salt-soda soil is varied, mainly has and soil conditioner, washing leaching, plantation is added In addition salt tolerant crop, biology --- plant combined improvement saline-alkali soil etc. will reinforce land management, strictly control dose, improves The quality of irrigation water, wherein peasant using it is more be plantation salt tolerant crop, be the part absorbed by crop in soil Salinity and have the function that reduce soil salt, the salt branch of absorption shifts with the harvesting of crop.In addition, also there is research aobvious Show that the interaction relationship between microorganism and plant is also beneficial to improve the saline and alkaline of soil, therefore, utilizes microorganism and plant Object is raw material to improve soil alkaline situation should be an environmentally protective desirable route.
In China, annual stalk total output is about hundred million tons of 8-10, is occupied first place in the world, especially northern wheat, corn and reed Reed is resourceful, widely distributed, and growth is concentrated;The yield of its stalk increases year by year with the raising of Per Unit Area Grain Yield and total yield, But corresponding treatment technology lag, thus brings a series of environment and social concern, as atmosphere pollution, fire, water body are rich Fosterization etc..The approach of the stalk of rural area processing at present is mostly burning, feed and the returning to the field of extensive style, and straw-returning is as Traditional Agricultural Important component in industry production, and relevant report has also affirmed the nutrient effects of straw-returning, crop yield effect, ring Border and ecological effect etc., but stalk enter after soil to a certain extent there is also Accumulation of Organic Acids, decomposition slowly and To the adverse effect of farming and agronomic conditions etc..For this purpose, technical research personnel are also constantly searching for more efficiently straw in the industry Stalk cropland application approach includes the following steps if 104193429 A of CN disclose a kind of method of stalk fermentation organic fertilizer:Institute It states after drying agricultural crop straw, puts into reaction kettle, launch zymophyte together while launching stalk;After dispensing, It adds water to and buries whole stalks;After adding water, reaction kettle is completely cut off into air, under 30 DEG C and 1.51-2.01MPa of pressure Anaerobic fermentation is carried out, the zymogenic amount is launch stalk volume 0.2%, and fermentation period is 7~9 days;Fermentation finishes Afterwards, that supernatant liquor is extracted into reservoir is spare, and remaining part attaches animal wastes fermentation, and the additive amount of animal wastes is to throw Put the 17% of stalk volume;Remaining part uniformly mixes remaining part with the part in reservoir after fermenting;The hair Yeast-like fungi is Bacillus circulans, bacillus licheniformis, bacillus megaterium, bacillus subtilis, Bacillus macerans, bifid bar It is one or more in bacterium, compressed years, Nocard's bacillus;The agricultural crop straw is rice straw, asparagus straws, rape straw Stalk, wheat stalk, maize straw it is one or more;Animal wastes be selected from chicken manure, pig manure, Oletum Columba livia it is one or more.It should Fertilizer prepared by method has certain beneficial microbe for participating in farm-forestry crop growth, and effect is good, can be in certain journey after use The volume increase for improveing soil on degree, improving crops.But this method, using anaerobic fermentation, straw utilization degree is low, non-dry state Fertilizer is not readily transportable and applies, and preparation process is cumbersome, poor to the improved effect of basic soil.Therefore, if can research and develop One kind is converted into that nutrient is comprehensive, fertility is strong, can effectively improve alkaline land soil and improve and make using stalk as primary raw material For the production technology of the preferable bio-feritlizer of the comprehensive performances such as yield, being utilized to local straw-returning has highly important meaning Justice.
Invention content
The object of the present invention is to provide a kind of preparation methods of stalk fermentation bio-feritlizer, to solve existing method preparation Straw biological fertilizer fertility is weaker, preparation process very complicated poor to the ameliorative of soil, and finished product is not readily transportable and applies With, be not easy to the problems such as industrialized production.
The purpose of the present invention is what is be achieved through the following technical solutions:A kind of preparation method of stalk fermentation bio-feritlizer, Include the following steps:
(a)It is 2-3 by weight by wheat stalk, maize straw and reed straw:1-2:3-5 is mixed, and is smashed, will be crushed Stalk batch mixing and water press 3.5-5.5:1 mixing, obtains fermentation raw material;
(b)Decomposed Mixed Microbes are inoculated in the fermentation raw material, watering adjusts water content to 55-60%, in heat-retaining condition Under, 40-65 DEG C of compost fermentation 7-15 days;Obtain decomposed material;The decomposed Mixed Microbes are the 0.5-3% of the fermentation raw material weight;Institute Decomposed Mixed Microbes are stated by following weight part ratio material composition:Her 15-25 parts of Sa zymotic fluid of east, 2-4 parts of lactobacillus plantarum liquid, 1.5-2.5 parts of bacillus subtilis bacterium solution, 1-3 parts of Trichoderma viride liquid, 1-2 parts of thermophilic bacillus licheniformis liquid and candida utili 0.5-1.5 parts of liquid;
(c)Sugar residue, sodium humate and poly-aspartate are sequentially added in the decomposed material, mixing sprays plant ash The pH value that aqueous solution adjusts mixture is 6.0-7.8, and water content is to 55-60%, under heat-retaining condition, 40- in water use regulation material 55 DEG C digest 1-2 days;Material must be digested;The decomposed material:Sugar residue:Sodium humate:The weight ratio of poly-aspartate is 30-40: 10-20:5-7:3-5;
(d)Trace element fertilizer and EDTA-2Na stabilizers are added in the enzymolysis material, is uniformly mixed, adds compound Bacterium is adjusted, water content to 55%, at 45-55 DEG C ferment 3-5 days by pack in water use regulation material;Obtain secondary fermentation material;The enzyme It is 80-100 that material, which is solved, with trace element fertilizer, EDTA-2Na stabilizers, the compound weight part ratio for adjusting bacterium:2-3:0.5-1:0.5- 1;The compound adjusting bacterium bag includes following weight part ratio substance:1.5-2.5 parts of bacillus megaterium liquid, Paenibacillus polymyxa 1-2 parts of 0.5-1 parts of liquid, 2-4 parts of azotobacter vinelandii liquid and lactobacillus plantarum liquid;
(e)By the secondary fermentation material low temperature drying, graininess straw are made by comminutor in the mixed raw material after drying Stalk fermenting organism fertilizer.
Step in the present invention(a)The weight ratio of the wheat stalk, maize straw and reed straw is preferably 2:1:5 is mixed It closes;The crushing refers to that wheat stalk, maize straw and its reed straw are crushed to 0.5-1.0cm.
Step in the present invention(b)The preferably following weight part ratio material composition of the decomposed Mixed Microbes:Her Sa zymophyte of east Liquid:Lactobacillus plantarum liquid:Bacillus subtilis bacterium solution:Trichoderma viride liquid:Thermophilic bacillus licheniformis liquid:Candida utili Liquid=22:3:2.5:3:1:1;Stirring was carried out every 2 days 1 time, and control temperature in compost and be no more than 65 DEG C during fermentation, if Temperature reaches 65 DEG C will carry out turning when the time is not every 2 days number.
Step in the present invention(c)The sugar residue is the glucose manufacture waste of recycling, can be beet sugar slag, corn The mixture of any one of sugar residue, sugarcane sugar residue, watermelon sugar residue or two or more arbitrary proportions.
Step in the present invention(c)The mass percent concentration of the plant ash aqueous solution is 10-15%.
Step in the present invention(d)Trace element fertilizer is the mixing of boron fertilizer, zinc fertilizer, manganese fertilizer, molydbenum fertilizer, copper fertilizer, iron fertilizer, cobalt fertilizer Fertilizer belongs to commercial product, comes from Hebei Chao Qun agricultural science and technologys Co., Ltd.
Step in the present invention(e)The low temperature drying refers to being dried to the moisture of material to 2-8% at 40-60 DEG C.
The present invention is vaccinated with specific decomposed mixed mainly using northern wheat stalk, reed straw and maize straw as raw material Combined bacteria, by the stalk of the zymotechnique fermentative degradation under preference temperature and time, and by specific enzymolysis process and filling, And carry out secondary fermentation by the way that compound adjusting bacterium is added so that the bio-feritlizer finally prepared not only contains higher nutrition Element, fertility is strong, can significantly improve plant growth and yield, and containing active more abundant beneficial bacterium, can be in difference The pH value and vigor that salt affected soil is improved in degree, improve the grain yield of alkaline land soil long-term cropping.In addition, testing It is more existing general surprisingly, it was found that bio-feritlizer prepared by the present invention has certain disease-resistant performance and insect prevention performance in journey Logical biological organic fertilizer has more application value.
Preparation method provided by the invention is at low cost, simple for process, and operability is strong, easy to utilize, obtained straw Stalk fermenting organism fertilizer fertility is high, dry state convenience in transport and application, can not only really realize a large amount of stalk resources and efficiently turn Change, turns waste into wealth, but also the alkalinity in salt-soda soil can be improved, increase the activity of soil, improve the resistance of crop, make salt-soda soil The crop of plantation is effectively increased production, and has very high economic benefit and social benefit.
Specific implementation mode
Following example is for present invention be described in more detail, but the invention is not limited in any way.
Various bacterium spreads cultivation in the present invention:
Her Sa zymophyte, lactobacillus plantarum, bacillus subtilis, Trichoderma viride, thermophilic lichens bud of activation east respectively Spore bacillus, candida utili, bacillus megaterium, Paenibacillus polymyxa, azotobacter vinelandii;It then will be various after activation Bacterium is fermented after being inoculated into industrial fermentation tank respectively;Strain after each activation is with opportunistic pathogen kind and the weight ratio of culture medium for 1:5000 Ratio be seeded to fermentation tank dilatation culture.Single culture living bacteria count is not in decomposed Mixed Microbes and compound adjusting bacterium after spreading cultivation Less than 300,000,000/gram, fermentation condition is respectively:
Her Sa zymophyte nutrient media components percentage composition by weight of east is glucose 2%, yeast extract 1%, peptone 2%, Remaining is water;180 r/min shaking table cultures 10-16 hours at 38 DEG C;
Lactobacillus plantarum nutrient media components percentage composition by weight be 0.5% yeast extract, 0.5% peptone, 5% brown sugar, 0.1% Tween-80,0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% calcium carbonate, remaining is water;Culture Base temperature is 35 DEG C, and incubation time is 24-28 hours;
Bacillus subtilis nutrient media components percentage composition by weight is glucose 6%, yeast extract 1%, peptone 0.2%, CaCO31%, remaining is water, and 30 DEG C are cultivated 48-52 hours;
Trichoderma viride nutrient media components percentage composition by weight is 6% corn stalk powder, 2% corn flour, 0.1% phosphoric acid hydrogen Dipotassium, 0.3% ammonium nitrate, 0.05% magnesium sulfate, 0.001% ferrous sulfate, 1% soluble starch, remaining is water;Culture medium temperature is 25 DEG C, incubation time is 165-175 hours;
Thermophilic bacillus licheniformis component percentage composition by weight be 0.5% yeast extract, 0.5% peptone, 5% brown sugar, 0.1% Tween-80,0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% calcium carbonate, remaining is water.Culture medium Temperature is 65 DEG C;Incubation time is 36-45 hours;
Candida utili nutrient media components percentage composition by weight is potato 15%, glucose 2%, remaining is water, training It is 30 DEG C to support temperature, is cultivated 24-32 hours;
Bacillus megaterium nutrient media components percentage composition by weight be 0.5% yeast extract, 0.5% peptone, 5% brown sugar, 0.1% Tween-80,0.05% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% calcium carbonate, remaining is water;Culture Base temperature is 35 DEG C, and incubation time is 24-36 hours;
Paenibacillus polymyxa nutrient media components percentage composition by weight is potato 15%, glucose 2%, remaining is water, Cultivation temperature is 32 DEG C, and incubation time is 48-52 hours;
Azotobacter vinelandii nutrient media components percentage composition by weight is glucose 1%, KH2PO40.02%, MgSO4·7H2O 0.02%, NaCl 0.02%, CaSO4·7H2O 0.02%, CaCO30.5%, remaining is distilled water, pH value 7.0-7.2, proliferation training The condition of supporting cultivates 24-48h under the conditions of 36 DEG C.
Embodiment 1
(1)It is 2 by weight by wheat stalk, maize straw and reed straw:1:5 mixing, smash to 0.5cm, by powder Broken stalk batch mixing presses 4 with water:1 mixing, obtains fermentation raw material;
(2)Decomposed Mixed Microbes are inoculated with by the inoculum concentration of fermentation raw material weight 2% in fermentation raw material, watering adjusts water content To 55%, under heat-retaining condition, 55 DEG C of compost fermentations 12 days obtain decomposed material;Wherein decomposed Mixed Microbes spread cultivation described in various bacterium Liquid is formed in following volumes ratio:Her Sa zymocyte liquid 22L of east, lactobacillus plantarum liquid 3L, bacillus subtilis bacterium solution 2.5L, Trichoderma viride liquid 3L, thermophilic bacillus licheniformis liquid 1L and candida utili liquid 1L;During fermentation stirring was carried out every 2 days 1 time, and in compost temperature close to 65 DEG C when add stirring primary;
(3)A certain amount of decomposed material is taken, is in mass ratio by decomposed material and sugar residue, sodium humate and poly-aspartate 35:20:5:4 mixing, mixing, the pH value that the plant ash aqueous solution that sprinkling mass percent concentration is 15% adjusts mixture are 6.5, watering adjust material in water content to 55%, under heat-retaining condition, 45 DEG C digest 2 days;Material must be digested;
(4)A certain amount of enzymolysis material is taken, by enzymolysis material and trace element fertilizer, EDTA-2Na stabilizers, compound adjusting Bacterium is 100 in mass ratio:3:0.5:1 is uniformly mixed, and for water content to 55%, pack ferments 3 at 50 DEG C in water use regulation material It;Secondary fermentation material, the compound adjusting bacterium liquid that spreads cultivation described in various bacterium are formed in following volumes ratio:Bacillus megaterium Liquid 2.5L, Paenibacillus polymyxa liquid 1L, azotobacter vinelandii liquid 4L and lactobacillus plantarum liquid 2L;
(5)Secondary fermentation material is dried at 40 DEG C to the moisture of material to 5%, the mixed raw material after drying is led to It crosses comminutor and graininess stalk fermentation bio-feritlizer is made.
Embodiment 2
(1)It is 3 by weight by wheat stalk, maize straw and reed straw:2:5 mixing, smash to 1cm, will crush Stalk batch mixing and water press 5.5:1 mixing, obtains fermentation raw material;
(2)Decomposed Mixed Microbes are inoculated with by the inoculum concentration of fermentation raw material weight 0.5% in fermentation raw material, watering adjusts aqueous Amount is to 55%, and under heat-retaining condition, 65 DEG C of compost fermentations 7 days obtain decomposed material;Wherein decomposed Mixed Microbes by various bacterium the expansion Liquid is trained to form in following volumes ratio:Her Sa zymocyte liquid 25L, lactobacillus plantarum liquid 4L, bacillus subtilis bacterium solution of east 2.5L, Trichoderma viride bacterium solution 3L, thermophilic bacillus licheniformis liquid 2L and candida utili liquid 1.5L;During fermentation every 2 It carries out stirring 1 time, and in compost temperature close to 65 DEG C when add stirring primary;
(3)A certain amount of decomposed material is taken, is in mass ratio by decomposed material and sugar residue, sodium humate and poly-aspartate 40:20:7:5 mixing, mixing, the pH value that the plant ash aqueous solution that sprinkling mass percent concentration is 15% adjusts mixture are 6.0, watering adjust material in water content to 55%, under heat-retaining condition, 55 DEG C digest 2 days;Material must be digested;
(4)A certain amount of enzymolysis material is taken, by enzymolysis material and trace element fertilizer, EDTA-2Na stabilizers, compound adjusting Bacterium is 100 in mass ratio:3:1:0.5 is uniformly mixed, and for water content to 55%, pack ferments 3 at 50 DEG C in water use regulation material It;Secondary fermentation material, the compound adjusting bacterium liquid that spreads cultivation described in various bacterium are formed in following volumes ratio:Bacillus megaterium Liquid 2.5L, Paenibacillus polymyxa liquid 1L, azotobacter vinelandii liquid 4L and lactobacillus plantarum liquid 1.5L;
(5)Secondary fermentation material is dried at 40 DEG C to the moisture of material to 8%, the mixed raw material after drying is led to It crosses comminutor and graininess stalk fermentation bio-feritlizer is made.
Embodiment 3
(1)It is 2 by weight by wheat stalk, maize straw and reed straw:1:3 mixing, smash to 0.8cm, by powder Broken stalk batch mixing presses 3.5 with water:1 mixing, obtains fermentation raw material;
(2)Decomposed Mixed Microbes are inoculated with by the inoculum concentration of fermentation raw material weight 2% in fermentation raw material, watering adjusts water content To 60%, under heat-retaining condition, 40 DEG C of compost fermentations 15 days obtain decomposed material;Wherein decomposed Mixed Microbes spread cultivation described in various bacterium Liquid is formed in following volumes ratio:Her Sa zymocyte liquid 15L of east, lactobacillus plantarum liquid 2L, bacillus subtilis bacterium solution 1.5L, Trichoderma viride liquid 1L, thermophilic bacillus licheniformis liquid 1L and candida utili liquid 0.5L;It was turned over during fermentation every 2 days Material 1 time, and in compost temperature close to 65 DEG C when add stirring primary;
(3)A certain amount of decomposed material is taken, is in mass ratio by decomposed material and sugar residue, sodium humate and poly-aspartate 30:10:5:3 mixing, mixing, the pH value that the plant ash aqueous solution that sprinkling mass percent concentration is 10% adjusts mixture are 7.8, watering adjust material in water content to 55%, under heat-retaining condition, 40 DEG C digest 2 days;Material must be digested;
(4)A certain amount of enzymolysis material is taken, by enzymolysis material and trace element fertilizer, EDTA-2Na stabilizers, compound adjusting Bacterium is 80 in mass ratio:2:0.5:0.8 is uniformly mixed, and for water content to 55%, pack ferments 5 at 45 DEG C in water use regulation material It;Obtain secondary fermentation material;The compound adjusting bacterium liquid that spreads cultivation described in various bacterium is formed in following volumes ratio:Bacillus megaterium Liquid 1.5L, Paenibacillus polymyxa liquid 0.5L, azotobacter vinelandii liquid 2L and lactobacillus plantarum liquid 1L;
(5)Secondary fermentation material is dried at 60 DEG C to the moisture of material to 4%, the mixed raw material after drying is led to It crosses comminutor and graininess stalk fermentation bio-feritlizer is made.
Embodiment 4
(1)It is 2 by weight by wheat stalk, maize straw and reed straw:1.5:4 mixing, smash to 1cm, by powder Broken stalk batch mixing presses 5 with water:1 mixing, obtains fermentation raw material;
(2)Decomposed Mixed Microbes are inoculated with by the inoculum concentration of fermentation raw material weight 2.5% in fermentation raw material, watering adjusts aqueous Amount is to 55%, and under heat-retaining condition, 60 DEG C of compost fermentations 10 days obtain decomposed material;Wherein decomposed Mixed Microbes by various bacterium the expansion Liquid is trained to form in following volumes ratio:Her Sa zymocyte liquid 20L, lactobacillus plantarum liquid 2.5L, bacillus subtilis bacterium solution of east 1.8L, Trichoderma viride liquid 2L, thermophilic bacillus licheniformis liquid 1.5L and candida utili liquid 1L;During fermentation every 2 days Carry out stirring 1 time, and in compost temperature close to 65 DEG C when add stirring primary;
(3)A certain amount of decomposed material is taken, is in mass ratio by decomposed material and sugar residue, sodium humate and poly-aspartate 35:15:5.5:4.5 mixing, mixing, the plant ash aqueous solution that sprinkling mass percent concentration is 12% adjust the pH value of mixture It is 7.0, water content is to 55% in watering adjusting material, and under heat-retaining condition, 50 DEG C digest 1 day;Material must be digested;
(4)A certain amount of enzymolysis material is taken, by enzymolysis material and trace element fertilizer, EDTA-2Na stabilizers, compound adjusting Bacterium is 90 in mass ratio:2.5:1:0.5 is uniformly mixed, and for water content to 55%, pack ferments 5 at 55 DEG C in water use regulation material It;Obtain secondary fermentation material;The compound adjusting bacterium liquid that spreads cultivation described in various bacterium is formed in following volumes ratio:Bacillus megaterium Liquid 2L, Paenibacillus polymyxa liquid 0.8L, azotobacter vinelandii liquid 3L and lactobacillus plantarum liquid 1L;
(5)Secondary fermentation material is dried at 55 DEG C to the moisture of material to 2%, the mixed raw material after drying is led to It crosses comminutor and graininess stalk fermentation bio-feritlizer is made.
Embodiment 5
(1)It is 2.5 by weight by wheat stalk, maize straw and reed straw:1:5 mixing are smashed to 0.5cm, will The stalk batch mixing of crushing presses 4 with water:1 mixing, obtains fermentation raw material;
(2)Decomposed Mixed Microbes are inoculated with by the inoculum concentration of fermentation raw material weight 1% in fermentation raw material, watering adjusts water content To 55%, under heat-retaining condition, 45 DEG C of compost fermentations 15 days obtain decomposed material;Wherein decomposed Mixed Microbes spread cultivation described in various bacterium Liquid is formed in following volumes ratio:Her Sa zymocyte liquid 25L of east, lactobacillus plantarum liquid 2L, bacillus subtilis bacterium solution 1.5L, Trichoderma viride liquid 1L, thermophilic bacillus licheniformis liquid 1L and candida utili liquid 0.5L;It was turned over during fermentation every 2 days Material 1 time, and in compost temperature close to 65 DEG C when add stirring primary;
(3)A certain amount of decomposed material is taken, is in mass ratio by decomposed material and sugar residue, sodium humate and poly-aspartate 40:10:7:3 mixing, mixing, the pH value that the plant ash aqueous solution that sprinkling mass percent concentration is 10% adjusts mixture are 6.5, watering adjust material in water content to 55%, under heat-retaining condition, 45 DEG C digest 1.5 days;Material must be digested;
(4)A certain amount of enzymolysis material is taken, by enzymolysis material and trace element fertilizer, EDTA-2Na stabilizers, compound adjusting Bacterium is 100 in mass ratio:2:0.5:1 is uniformly mixed, and for water content to 55%, pack ferments 5 at 50 DEG C in water use regulation material It;Obtain secondary fermentation material;The compound adjusting bacterium liquid that spreads cultivation described in various bacterium is formed in following volumes ratio:Bacillus megaterium Liquid 2L, Paenibacillus polymyxa liquid 1L, azotobacter vinelandii liquid 3.5L and lactobacillus plantarum liquid 1L;
(5)Secondary fermentation material is dried at 55 DEG C to the moisture of material to 2%, the mixed raw material after drying is led to It crosses comminutor and graininess stalk fermentation bio-feritlizer is made.
Embodiment 6
(1)It is 2 by weight by wheat stalk, maize straw and reed straw:1:3 mixing, smash to 0.5cm, by powder Broken stalk batch mixing presses 5.5 with water:1 mixing, obtains fermentation raw material;
(2)Decomposed Mixed Microbes are inoculated with by the inoculum concentration of fermentation raw material weight 3% in fermentation raw material, watering adjusts water content To 60%, under heat-retaining condition, 55 DEG C of compost fermentations 12 days obtain decomposed material;Wherein decomposed Mixed Microbes spread cultivation described in various bacterium Liquid is formed in following volumes ratio:Her Sa zymocyte liquid 20L of east, lactobacillus plantarum liquid 4L, bacillus subtilis bacterium solution 2.5L, Trichoderma viride liquid 3L, thermophilic bacillus licheniformis liquid 1L and candida utili liquid 0.5L;It was turned over during fermentation every 2 days Material 1 time, and in compost temperature close to 65 DEG C when add stirring primary;
(3)A certain amount of decomposed material is taken, is in mass ratio by decomposed material and sugar residue, sodium humate and poly-aspartate 35:20:6:3 mixing, mixing, the pH value that the plant ash aqueous solution that sprinkling mass percent concentration is 15% adjusts mixture are 7.2, watering adjust material in water content to 55%, under heat-retaining condition, 45 DEG C digest 1.5 days;Material must be digested;
(4)A certain amount of enzymolysis material is taken, is in mass ratio with trace element fertilizer, EDTA-2Na stabilizers by enzymolysis material 100:3:0.5:0.5 is uniformly mixed, and water content to 55%, at 55 DEG C ferment 4 days by pack in water use regulation material;Obtain secondary hair Ferment material;The compound adjusting bacterium liquid that spreads cultivation described in various bacterium is formed in following volumes ratio:Bacillus megaterium liquid 2L, it glues more Series bacillus liquid 1L, azotobacter vinelandii liquid 4L and lactobacillus plantarum liquid 1L;
(5)Secondary fermentation material is dried at 50 DEG C to the moisture of material to 6%, the mixed raw material after drying is led to It crosses comminutor and graininess stalk fermentation bio-feritlizer is made.
Comparative example 1
The bio-feritlizer of mixed fungus fermentation stalk in the prior art:It is 2 by weight by wheat stalk and maize straw:1 is mixed It closes, smashes, the stalk batch mixing of crushing and water are pressed 4:1 mixing, obtains fermentation raw material;Fermentation raw material weight 2% is pressed in fermentation raw material Inoculum concentration be inoculated with decomposed Mixed Microbes, watering is adjusted to water content to 55%, and under heat-retaining condition, 55 DEG C of compost fermentations 15 days obtain Decomposed material;The wherein decomposed Mixed Microbes liquid that spreads cultivation described in various bacterium is formed in following volumes ratio:Lactobacillus plantarum liquid 5L, Bacillus subtilis bacterium solution 2L, Trichoderma viride liquid 3L, thermophilic bacillus licheniformis liquid 1L, candida utili liquid 0.5L, huge bud Spore bacillus liquid 2.5L, Paenibacillus polymyxa liquid 1L and azotobacter vinelandii liquid 4L;It must be turned within every two days or three days in fermentation process Mixed once material, with the pH values of plant ash water management mixed material between 6 to 7.8;Be granulated, dried after fermentation, Up to bio-feritlizer.
Embodiment 7 detects the application effect of bio-feritlizer prepared by the present invention and comparative example.
For trying wheat breed:Ji 5418
1-6 of the embodiment of the present invention prepares bio-feritlizer, bio-feritlizer prepared by comparative example 1 and blank control at totally 8 Reason, each to handle 100 square metres of plot area, sowing ground is Cangzhou Nanpi salt-soda soil.The fertilizer that the present invention and comparative example are applied The method of material is:It all uniformly spreads fertilizer over the fields to turn over after earth's surface before wheat cultivation using machinery and bury, apply fertilizer 80 kg per cell(8 t/hm2), no longer impose urea;Blank control refers to not applying the processing of any fertilizer.10 the first tenday period of a month of wheat are sowed, and adopt according to demand Collect experimental group, the data of contrast groups and control group.
Each processing chooses 5 square metres and uses the manual-induced banded sclerotial blight of seed furrow soil bacterination process.By rhizoctonia cerealis Culture to wheat covers with mycelia in inoculation to the wheat seed of high pressure steam sterilization.When wheat cultivation, in seed furrow It is even to broadcast sowing the cultured wheat that carries disease germs, then the seed item of experimental cultivar is sowed in seed furrow, last earthing, water spray moisturizing 1 week. The cultured wheat that carries disease germs uniformly is spread on Mai Hangzhong, is connect bacterium for the second time, and intertill mid-March next year, moisturizing one of spraying water The section time ensures that morbidity is abundant.
(1)Yield is investigated:Each processing randomly selects 52 square metres of plot, grain yield is weighed after harvesting, and change Calculate the yield-increasing efficiency of each processing.
Rate of growth %=(Experimental group gross production rate-blank control group total output)/ blank control group total output * 100
(2)Banded sclerotial blight is investigated:Second year investigates the state of an illness mid-May, pulls out strain investigation, every group of experiment duplication before harvest again 30 plants, grade scale is as follows:0 grade:It is disease-free;1 grade:Scab is around 1/4 or less stem;2 grades:Scab is around stem 1/4~1/2;3 grades:Scab Around stem 1/2~3/4;4 grades:Scab is not but withered around 3/4 or more stem;5 grades:Plant is withered or generates dead ears.
Incidence=[Σ (morbidity grade × plant quantity)/(total plant quantity × highest levels)] × 100%
Control rate=[(control incidence-bio-feritlizer handles incidence)/control incidence] × 100%
(3)Soil acid-base sex investigation:
The PH of each processing soil is measured in sowing and harvest respectively.Each processing chooses 20 points along cell diagonal line, Soil pH is directly read on pH meter with potentiometry.
Experimental result is shown in Table 1.
The Indexs measure result of 1 each embodiment of table and comparative example
The method of administration of bio-feritlizer provided by the present invention can uniformly spread fertilizer over the fields it in alkaline land soil table to be improved Face determines dosage according to the actual conditions in salt-soda soil, then is turned over rotary cultivator, and improvement fertilizer is made to be filled with alkaline land soil to be improved Tap is touched.Such as coordinate plantation Saline alkali tolerance crop, preferable effect is all had to improvement soil and raising yield.In addition to this, The organic fertilizer prepared during the experiment using the present invention has the characteristic of certain mosquito-proof sting compared with comparative example and blank.

Claims (4)

1. a kind of preparation method of stalk fermentation bio-feritlizer, which is characterized in that include the following steps:
(a)It is 2-3 by weight by wheat stalk, maize straw and reed straw:1-2:3-5 is mixed, and is smashed, by the straw of crushing Stalk batch mixing presses 3.5-5.5 with water:1 mixing, obtains fermentation raw material;
(b)Decomposed Mixed Microbes are inoculated in the fermentation raw material, watering adjusts water content to 55-60%, under heat-retaining condition, 40- 65 DEG C of compost fermentations 7-15 days;Obtain decomposed material;The decomposed Mixed Microbes are the 0.5-3% of the fermentation raw material weight;It is described decomposed Mixed Microbes are by following weight part ratio material composition:Her 15-25 parts of Sa zymotic fluid, 2-4 parts of lactobacillus plantarum liquid, withered grass bud of east 1.5-2.5 parts of spore bacillus liquid, 1-3 parts of Trichoderma viride liquid, 1-2 parts of thermophilic bacillus licheniformis liquid and candida utili liquid 0.5- 1.5 part;
(c)Sugar residue, sodium humate and poly-aspartate are sequentially added in the decomposed material, it is water-soluble to spray plant ash for mixing The pH value that liquid adjusts mixture is 6.0-7.8, and water content is to 55-60%, under heat-retaining condition, 40-55 DEG C in water use regulation material Enzymolysis 1-2 days;Material must be digested;The decomposed material:Sugar residue:Sodium humate:The weight ratio of poly-aspartate is 30-40:10- 20:5-7:3-5;
(d)Trace element fertilizer and EDTA-2Na stabilizers are added in the enzymolysis material, is uniformly mixed, adds compound adjusting Bacterium, water content to 55%, at 45-55 DEG C ferment 3-5 days by pack in water use regulation material;Obtain secondary fermentation material;The zymolyte Material is 80-100 with trace element fertilizer, EDTA-2Na stabilizers, the compound weight part ratio for adjusting bacterium:2-3:0.5-1:0.5-1;Institute It states compound adjusting bacterium bag and includes following weight part ratio substance:1.5-2.5 parts of bacillus megaterium liquid, Paenibacillus polymyxa liquid 1-2 parts of 0.5-1 parts, 2-4 parts of azotobacter vinelandii liquid and lactobacillus plantarum liquid;
(e)By the secondary fermentation material low temperature drying, graininess stalk hair is made by comminutor in the mixed raw material after drying Ferment bio-feritlizer.
2. the preparation method of stalk fermentation bio-feritlizer according to claim 1, which is characterized in that step(a)Described Crushing refers to that wheat stalk, maize straw and reed straw are crushed to 0.5-1.0cm.
3. the preparation method of stalk fermentation bio-feritlizer according to claim 1, which is characterized in that in step(b)Hair During ferment stirring was carried out every 2 days 1 time.
4. the preparation method of stalk fermentation bio-feritlizer according to claim 1, which is characterized in that step(e)It is described low Warm drying refer at 40-60 DEG C drying to the moisture of material to 2-8%.
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CN107759397A (en) * 2016-08-18 2018-03-06 曹芳 A kind of agrochemical
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