CN112645746A - Animal manure straw bio-organic fertilizer and preparation method thereof - Google Patents

Animal manure straw bio-organic fertilizer and preparation method thereof Download PDF

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CN112645746A
CN112645746A CN202011566448.1A CN202011566448A CN112645746A CN 112645746 A CN112645746 A CN 112645746A CN 202011566448 A CN202011566448 A CN 202011566448A CN 112645746 A CN112645746 A CN 112645746A
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animal manure
straw
organic fertilizer
liquid
parts
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李凤兰
张世宏
刘娣
何流琴
冯艳忠
袁强
冯哲
李柱刚
吴建
杨秀梅
梁飞
田文会
常婷婷
杨劲松
姜雪峰
贺付蒙
赫兰保
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Northeast Agricultural University
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention discloses an animal manure straw bio-organic fertilizer and a preparation method thereof. The raw material components by weight are as follows: 60-70 parts of animal manure, 30-40 parts of straws, 0.2-0.3 part of straw decomposition agent and 0.1-0.2 part of probiotic liquid. The method comprises the following steps: mixing bacillus licheniformis, lactobacillus casei and saccharomyces cerevisiae to obtain probiotic liquid, and mixing bacillus subtilis, aspergillus oryzae and candida utilis to obtain a straw decomposition agent; mixing the raw material components to obtain a mixed material; the initial pH of the material is natural, the fermentation is carried out for 90-120 days at the temperature higher than 4 ℃, and the fermentation is finished when the number of faecal coliform bacteria in the material is less than or equal to 50, so as to obtain the biological organic fertilizer. The invention can convert animal manure urine and straws into organic fertilizer, and can promote the growth of crops and improve the yield of the crops by applying the fertilizer.

Description

Animal manure straw bio-organic fertilizer and preparation method thereof
Technical Field
The invention belongs to the technical field of fertilizers, and particularly relates to an animal manure straw bio-organic fertilizer and a preparation method thereof.
Background
In recent years, with the development of economy in China and the improvement of the living standard of people, the demand of domestic markets for animal products is continuously increased, the rapid development of animal breeding industry is promoted, and large-scale and industrialized farms and breeding enterprises grow rapidly. However, animals produce a large amount of waste materials such as feces and urine during their growth, and a large amount of organic substances are contained in the waste materials, so that the direct discharge of the waste materials causes waste of resources and serious environmental pollution. Therefore, how to effectively treat the wastes and reduce environmental pollution becomes a difficult problem for farms and breeding enterprises.
In addition, the planting industry generates a large amount of straws every year while providing people with abundant agricultural products such as grains and vegetables. Because the straws contain a large amount of organic matters, if the straws cannot be effectively treated, the waste of resources and environmental pollution can be caused, for example, the smoke generated by straw burning can pollute the air.
Therefore, the animal excrement urine and the straws are converted into the organic fertilizer required by the growth of crops, and the organic fertilizer has great significance for resource utilization of the animal excrement urine and the straws and improvement of soil fertility.
Disclosure of Invention
The invention aims to solve the technical problem of providing an animal manure straw bio-organic fertilizer and a preparation method thereof, which convert animal manure urine and straws into organic fertilizers through fermentation, realize the recycling treatment of the animal manure urine and straws and reduce the environmental pollution.
On one hand, the invention provides an animal manure straw bio-organic fertilizer which is prepared from raw materials consisting of animal manure urine, straws, a straw decomposition agent and a composite probiotic liquid.
Preferably, the animal manure straw bio-organic fertilizer is prepared by uniformly mixing the raw materials and then carrying out facultative fermentation for 90-120 days at the temperature higher than 4 ℃, wherein the number of faecal coliform bacteria in the animal manure straw bio-organic fertilizer is less than or equal to 50.
Preferably, the raw materials comprise, by weight, 60-70 parts of the animal manure, 30-40 parts of the straws, 0.2-0.3 part of the straw decomposition agent and 0.1-0.2 part of the compound probiotic liquid.
Preferably, the raw materials comprise, by weight, 60 parts of the animal manure, 33 parts of the straws, 0.23 part of the straw decomposition agent and 0.1 part of the compound probiotic liquid.
Preferably, the raw materials comprise 63 parts of animal manure urine, 30 parts of straws, 0.2 part of straw decomposition agent and 0.14 part of composite probiotic liquid by weight.
Preferably, the raw materials comprise 67 parts of animal manure urine, 40 parts of straws, 0.3 part of straw decomposition agent and 0.18 part of composite probiotic liquid by weight.
Preferably, the raw materials comprise 70 parts of animal manure urine, 37 parts of straws, 0.26 part of straw decomposition agent and 0.2 part of composite probiotic liquid by weight.
Preferably, the composite probiotic liquid comprises bacillus licheniformis, lactobacillus casei and saccharomyces cerevisiae, and the straw decomposition agent comprises bacillus subtilis, aspergillus oryzae and candida utilis.
Preferably, the bacteria content of the bacillus licheniformis is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 108cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the aspergillus oryzae is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis is more than or equal to 3.0 multiplied by 109cfu/g。
On the other hand, the invention also provides a preparation method of the animal manure straw bio-organic fertilizer, which comprises the following steps:
step 1, preparing a composite probiotic liquid and a straw decomposition agent, mixing a bacillus licheniformis liquid, a lactobacillus casei liquid and a saccharomyces cerevisiae liquid to obtain the composite probiotic liquid, and mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder to obtain the straw decomposition agent;
step 2, preparing a mixed material, and uniformly mixing animal manure urine, straws, the straw decomposition agent and the composite probiotic liquid to obtain the mixed material;
and 3, fermenting, wherein the initial pH of the material is natural, performing facultative fermentation for 90-120 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform groups in the fermented material is less than or equal to 50, and taking the fermented product as the animal manure straw bio-organic fertilizer.
The animal manure straw bio-organic fertilizer can convert animal manure urine and straws into organic fertilizer through fermentation, realizes the recycling of the animal manure urine and the straws, reduces the environmental pollution, and can promote the growth of crops and improve the crop yield by applying the animal manure straw bio-organic fertilizer.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The animal manure and straw bio-organic fertilizer is prepared by mixing animal manure urine and straws and fermenting by adopting probiotics and a straw decomposition agent (for convenience, the bio-organic fertilizer is hereinafter referred to as bio-organic fertilizer). The animal manure urine and the straws are converted into the organic fertilizer through fermentation, so that the recycling of the animal manure urine and the straws is realized, the environmental pollution is reduced, the crop growth can be promoted by applying the biological organic fertilizer, and the crop yield is increased.
In a specific embodiment of the invention, the bio-organic fertilizer is prepared from raw materials consisting of animal manure, straw decomposition agent and composite probiotic liquid. The specific preparation process comprises the following steps: step 1, preparing a composite probiotic liquid and a straw decomposition agent; step 2, preparing a mixed material; and 3, fermenting the mixed material.
In the step 1 of preparing the composite probiotic liquid and the straw decomposition agent, on one hand, a Bacillus licheniformis (Bacillus licheniformis) liquid, a Lactobacillus casei (Lactobacillus casei) liquid and a Saccharomyces cerevisiae (Saccharomyces cerevisiae) liquid are selected, and then the three kinds of liquid are compounded according to the proportion relation of weight to obtain the composite probiotic liquid, preferably, the Bacillus licheniformis liquid is selected25-40 parts of bacterial liquid, 30-45 parts of lactobacillus casei bacterial liquid and 25-35 parts of saccharomyces cerevisiae bacterial liquid. Wherein the bacterial content of the bacillus licheniformis liquid is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei bacterium liquid is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL. The three kinds of bacterial liquids can be prepared by self, and the bacillus licheniformis, the lactobacillus casei and the saccharomyces cerevisiae are respectively subjected to high-density fermentation culture in corresponding liquid culture media to obtain the bacterial liquids.
On the other hand, selecting Bacillus subtilis powder, Aspergillus oryzae (Aspergillus oryzae) powder and Candida utilis (Candida utilis) powder, and then compounding the three kinds of powder according to the weight proportion to obtain the straw decomposing inoculant, wherein 25-35 parts of the Bacillus subtilis powder, 30-40 parts of the Aspergillus oryzae powder and 20-30 parts of the Candida utilis powder are preferably selected. Wherein the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the Aspergillus oryzae bacterium powder is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis powder is more than or equal to 3.0 multiplied by 109cfu/mL. The three kinds of bacteria powder can be prepared by themselves. On one hand, bacillus subtilis and candida utilis are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids, and then thalli are separated from fermentation cultures, dried and concentrated to prepare single-strain solid bacterial powder. On the other hand, Aspergillus oryzae is fermented in a corresponding solid culture medium to obtain a solid culture, and then the solid culture is dried and crushed to prepare single-strain solid bacterial powder.
In the step 2 of preparing the mixed material, various raw material components are weighed according to a preset weight ratio, and then all the raw materials are mixed and stirred uniformly. The weight ratio of each component in the raw materials is respectively optimized as follows: 60-70 parts of animal manure, 30-40 parts of straws, 0.2-0.3 part of straw decomposition agent and 0.1-0.2 part of composite probiotic liquid. Wherein the straw is preferably a particle with the length of 3cm-5 cm.
And 3, fermenting the mixed material, namely performing aerobic fermentation on the mixed material at the initial pH of the mixed material for 90-120 days at the temperature of higher than 4 ℃, ending the fermentation when the number of faecal coliform groups in the fermented material is less than or equal to 50, and taking the fermented product as the bio-organic fertilizer.
To help better understand the technical scheme of the present invention, the following examples are provided for illustrating the bio-organic fertilizer and the preparation process thereof according to the present invention.
Example one
The raw materials of the bio-organic fertilizer comprise, by weight, 60 parts of animal manure, 33 parts of straws, 0.23 part of straw decomposition agent and 0.1 part of composite probiotic liquid. The composite probiotic liquid is formed by mixing bacillus licheniformis liquid, lactobacillus casei liquid and saccharomyces cerevisiae liquid, wherein the three liquid are respectively 25 parts by weight of the bacillus licheniformis liquid, 40 parts by weight of the lactobacillus casei liquid and 35 parts by weight of the saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacteria content of the bacillus licheniformis liquid is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei bacterium liquid is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL. The straw decomposing inoculant is prepared by mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder, wherein the three kinds of the bacteria powder are 25 parts of the bacillus subtilis powder, 36 parts of the aspergillus oryzae powder and 27 parts of the candida utilis powder by weight. The bacterium contents of the three bacterium powders are respectively as follows: the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the Aspergillus oryzae bacterium powder is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis powder is more than or equal to 3.0 multiplied by 109cfu/mL。
The biological organic fertilizer is prepared by the following steps.
Step 1, preparing a composite probiotic liquid and a straw decomposition agent, and uniformly mixing a bacillus licheniformis liquid, a lactobacillus casei liquid and a saccharomyces cerevisiae liquid to obtain the composite probiotic liquid; uniformly mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder to obtain the straw decomposing inoculant.
And 2, mixing the raw materials to prepare a mixed material, adding the animal manure urine, the straws, the straw decomposition agent and the composite probiotic liquid together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, carrying out facultative fermentation for 90 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform bacteria in the fermented material is less than or equal to 50, and taking the fermented product as the bio-organic fertilizer 1.
Example two
The raw materials of the bio-organic fertilizer comprise 63 parts of animal manure urine, 30 parts of straws, 0.2 part of straw decomposition agent and 0.14 part of composite probiotic liquid by weight. The composite probiotic liquid is formed by mixing bacillus licheniformis liquid, lactobacillus casei liquid and saccharomyces cerevisiae liquid, wherein the three liquid are 30 parts of the bacillus licheniformis liquid, 45 parts of the lactobacillus casei liquid and 29 parts of the saccharomyces cerevisiae liquid respectively. The bacterial contents of the three bacterial liquids are respectively as follows: the bacteria content of the bacillus licheniformis liquid is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei bacterium liquid is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL. The straw decomposing inoculant is prepared by mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder, wherein the three kinds of the bacteria powder are 29 parts of the bacillus subtilis powder, 40 parts of the aspergillus oryzae powder and 23 parts of the candida utilis powder respectively by weight. The bacterium contents of the three bacterium powders are respectively as follows: the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the Aspergillus oryzae bacterium powder is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis powder is more than or equal to 3.0 multiplied by 109cfu/mL。
The biological organic fertilizer is prepared by the following steps.
Step 1, preparing a composite probiotic liquid and a straw decomposition agent, and uniformly mixing a bacillus licheniformis liquid, a lactobacillus casei liquid and a saccharomyces cerevisiae liquid to obtain the composite probiotic liquid; uniformly mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder to obtain the straw decomposing inoculant.
And 2, mixing the raw materials to prepare a mixed material, adding the animal manure urine, the straws, the straw decomposition agent and the composite probiotic liquid together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, carrying out facultative fermentation for 100 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform bacteria in the fermented material is less than or equal to 50, and taking the fermented product as a biological organic fertilizer 2.
EXAMPLE III
The raw materials of the bio-organic fertilizer comprise 67 parts of animal manure urine, 40 parts of straws, 0.3 part of straw decomposition agent and 0.18 part of composite probiotic liquid by weight. The composite probiotic liquid is formed by mixing bacillus licheniformis liquid, lactobacillus casei liquid and saccharomyces cerevisiae liquid, wherein the three liquid are 35 parts of the bacillus licheniformis liquid, 37 parts of the lactobacillus casei liquid and 25 parts of the saccharomyces cerevisiae liquid respectively. The bacterial contents of the three bacterial liquids are respectively as follows: the bacteria content of the bacillus licheniformis liquid is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei bacterium liquid is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL. The straw decomposing inoculant is prepared by mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder, wherein the three kinds of the bacteria powder are 35 parts of the bacillus subtilis powder, 33 parts of the aspergillus oryzae powder and 20 parts of the candida utilis powder respectively by weight. The bacterium contents of the three bacterium powders are respectively as follows: the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the Aspergillus oryzae bacterium powder is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis powder is more than or equal to 3.0 multiplied by 109cfu/mL。
The biological organic fertilizer is prepared by the following steps.
Step 1, preparing a composite probiotic liquid and a straw decomposition agent, and uniformly mixing a bacillus licheniformis liquid, a lactobacillus casei liquid and a saccharomyces cerevisiae liquid to obtain the composite probiotic liquid; uniformly mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder to obtain the straw decomposing inoculant.
And 2, mixing the raw materials to prepare a mixed material, adding the animal manure urine, the straws, the straw decomposition agent and the composite probiotic liquid together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, performing facultative fermentation for 110 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform bacteria in the fermented material is less than or equal to 50, and taking the fermented product as a biological organic fertilizer 3.
Example four
The raw materials of the bio-organic fertilizer comprise 70 parts of animal manure urine, 37 parts of straws, 0.26 part of straw decomposition agent and 0.2 part of composite probiotic liquid by weight. The composite probiotic liquid is formed by mixing bacillus licheniformis liquid, lactobacillus casei liquid and saccharomyces cerevisiae liquid, wherein the three kinds of liquid are 40 parts by weight of the bacillus licheniformis liquid, 30 parts by weight of the lactobacillus casei liquid and 27 parts by weight of the saccharomyces cerevisiae liquid. The bacterial contents of the three bacterial liquids are respectively as follows: the bacteria content of the bacillus licheniformis liquid is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei bacterium liquid is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 4.0 multiplied by 108cfu/mL. The straw decomposing inoculant is prepared by mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder, wherein the three kinds of the bacteria powder are 33 parts of the bacillus subtilis powder, 30 parts of the aspergillus oryzae powder and 30 parts of the candida utilis powder respectively by weight. The bacterium contents of the three bacterium powders are respectively as follows: the bacterial content of the bacillus subtilis powder is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the Aspergillus oryzae bacterium powder is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis powder is more than or equal to 3.0 multiplied by 109cfu/mL。
The biological organic fertilizer is prepared by the following steps.
Step 1, preparing a composite probiotic liquid and a straw decomposition agent, and uniformly mixing a bacillus licheniformis liquid, a lactobacillus casei liquid and a saccharomyces cerevisiae liquid to obtain the composite probiotic liquid; uniformly mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder to obtain the straw decomposing inoculant.
And 2, mixing the raw materials to prepare a mixed material, adding the animal manure urine, the straws, the straw decomposition agent and the composite probiotic liquid together, stirring and uniformly mixing.
And 3, fermenting, wherein the initial pH of the material is natural, performing facultative fermentation for 120 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform bacteria in the fermented material is less than or equal to 50, and taking the fermented product as a bio-organic fertilizer 4.
In order to help better understand the technical scheme of the invention, a test example of rice planting is provided below for illustrating the application effect of the invention.
The first test example: influence of bio-organic fertilizer on rice growth
A rice planting test is carried out in the same region of Daqing city, the same field is selected, the basic physicochemical property of field soil is pH value 7.46, the organic matter content of the soil of a plough layer is 26.5g/kg, the quick-acting phosphorus amount is 28mg/kg, the quick-acting potassium amount is 185mg/kg, and the alkaline hydrolysis nitrogen amount is 126 mg/kg. The experimental design 5 groups comprise 4 experimental groups and 1 control group, each group of experimental designs comprises 3 experimental cells, and the area of each experimental cell is 20m2All test cells are randomly distributed.
The test planting variety is Longdao No. 28, the bowl tray seedling culture is adopted in the first ten days of 4 months, the artificial seedling transplanting is carried out in the middle ten days of 5 months, 5 seedlings/hole, the plant distance is 10cm, and the row distance is 30 cm. The control group was fertilized according to the conventional method with urea 180kg/hm2120kg/hm of diammonium phosphate2And potassium sulfate 75kg/hm2The nitrogen fertilizer is applied according to the ratio of base fertilizer to tiller fertilizer to spike fertilizer to 4:3:3, the potassium fertilizer is applied according to the ratio of base fertilizer to spike fertilizer to 1:1, and the phosphate fertilizer is applied as the base fertilizer. Test group applied with 90kg/hm urea260kg/hm diammonium phosphate2And potassium sulfate 37.5kg/hm2The application mode is the same as that of a control group, and the prepared biological organic fertilizer is applied as a base fertilizer at the same time, wherein the fertilizing amount is 1500kg/hm2. The same general management is adopted for each cell, and the cells are harvested in the last 10 months.
Before harvesting rice, randomly and continuously taking 10 plants from each cell for indoor seed test, and calculating the average plant height, the average effective spike number, the average solid grain number per spike and the average thousand grain weight of each group; after harvesting the rice, the yield of each cell was counted and the average cell yield per group was calculated. The results are shown in Table 1.
TABLE 1
Figure BDA0002861137750000071
Figure BDA0002861137750000081
As can be seen from the data in Table 1, the 4 groups of rice applied with the bio-organic fertilizer are obviously higher than the control group in five properties of plant height, effective spike number, solid grain number per spike, thousand grain weight and cell yield. Therefore, the prepared bio-organic fertilizer 1-4 can obviously promote the growth of rice and improve the yield of rice.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.

Claims (10)

1. An animal manure straw bio-organic fertilizer is prepared from raw materials consisting of animal manure urine, straws, a straw decomposition agent and a composite probiotic liquid.
2. The animal manure straw bio-organic fertilizer of claim 1, which is characterized in that: the animal manure straw bio-organic fertilizer is prepared by uniformly mixing the raw materials and then carrying out facultative fermentation for 90-120 days at the temperature higher than 4 ℃, wherein the number of faecal coliform groups in the animal manure straw bio-organic fertilizer is less than or equal to 50.
3. The animal manure straw bio-organic fertilizer of claim 1, which is characterized in that: the raw materials comprise, by weight, 60-70 parts of the animal manure urine, 30-40 parts of the straw, 0.2-0.3 part of the straw decomposition agent and 0.1-0.2 part of the composite probiotic liquid.
4. The animal manure straw bio-organic fertilizer of claim 3, which is characterized in that: the raw materials comprise, by weight, 60 parts of animal manure urine, 33 parts of straws, 0.23 part of straw decomposition agent and 0.1 part of composite probiotic liquid.
5. The animal manure straw bio-organic fertilizer of claim 3, which is characterized in that: the raw materials comprise 63 parts of animal manure urine, 30 parts of straws, 0.2 part of straw decomposition agent and 0.14 part of composite probiotic liquid by weight.
6. The animal manure straw bio-organic fertilizer of claim 3, which is characterized in that: the raw materials comprise 67 parts of animal manure urine, 40 parts of straws, 0.3 part of straw decomposition agent and 0.18 part of composite probiotic liquid by weight.
7. The animal manure straw bio-organic fertilizer of claim 3, which is characterized in that: the raw materials comprise 70 parts of animal manure urine, 37 parts of straws, 0.26 part of straw decomposition agent and 0.2 part of composite probiotic liquid by weight.
8. The animal manure straw bio-organic fertilizer according to any one of claims 1 to 7, wherein: the composite probiotic liquid comprises bacillus licheniformis, lactobacillus casei and saccharomyces cerevisiae, and the straw decomposition agent comprises bacillus subtilis, aspergillus oryzae and candida utilis.
9. The animal manure straw bio-organic fertilizer of claim 8, which is characterized in that: the bacteria content of the bacillus licheniformis is more than or equal to 1.0 multiplied by 1010cfu/mL, the bacterium content of the lactobacillus casei is more than or equal to 5.0 multiplied by 1011cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 108cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 2.0 multiplied by 1010cfu/g, the bacterium content of the aspergillus oryzae is more than or equal to 4.0 multiplied by 109cfu/g, the bacteria content of the candida utilis is more than or equal to 3.0 multiplied by 109cfu/g。
10. A preparation method of an animal manure straw bio-organic fertilizer is characterized by comprising the following steps: the preparation method comprises the following steps:
step 1, preparing a composite probiotic liquid and a straw decomposition agent, mixing a bacillus licheniformis liquid, a lactobacillus casei liquid and a saccharomyces cerevisiae liquid to obtain the composite probiotic liquid, and mixing bacillus subtilis powder, aspergillus oryzae powder and candida utilis powder to obtain the straw decomposition agent;
step 2, preparing a mixed material, and uniformly mixing animal manure urine, straws, the straw decomposition agent and the composite probiotic liquid to obtain the mixed material;
and 3, fermenting, wherein the initial pH of the material is natural, performing facultative fermentation for 90-120 days at the temperature higher than 4 ℃, finishing fermentation when the number of faecal coliform groups in the fermented material is less than or equal to 50, and taking the fermented product as the animal manure straw bio-organic fertilizer.
CN202011566448.1A 2020-12-25 2020-12-25 Animal manure straw bio-organic fertilizer and preparation method thereof Pending CN112645746A (en)

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CN112500216A (en) * 2020-11-02 2021-03-16 东北农业大学 Bio-organic fertilizer with crop rooting promoting function and preparation method thereof
CN112960994A (en) * 2021-03-12 2021-06-15 东北农业大学 Method for preparing organic fertilizer by fermenting straw and sludge
CN113372148A (en) * 2021-07-22 2021-09-10 创想未来生物工程(北京)有限公司 Organic material decomposing microbial agent and preparation method thereof
CN115504841A (en) * 2022-11-02 2022-12-23 安徽省农业科学院棉花研究所 Fermented pig manure organic fertilizer and production method thereof

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CN112500216A (en) * 2020-11-02 2021-03-16 东北农业大学 Bio-organic fertilizer with crop rooting promoting function and preparation method thereof
CN112960994A (en) * 2021-03-12 2021-06-15 东北农业大学 Method for preparing organic fertilizer by fermenting straw and sludge
CN113372148A (en) * 2021-07-22 2021-09-10 创想未来生物工程(北京)有限公司 Organic material decomposing microbial agent and preparation method thereof
CN115504841A (en) * 2022-11-02 2022-12-23 安徽省农业科学院棉花研究所 Fermented pig manure organic fertilizer and production method thereof

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