CN112321338A - Biological organic fertilizer - Google Patents

Biological organic fertilizer Download PDF

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Publication number
CN112321338A
CN112321338A CN202011010650.6A CN202011010650A CN112321338A CN 112321338 A CN112321338 A CN 112321338A CN 202011010650 A CN202011010650 A CN 202011010650A CN 112321338 A CN112321338 A CN 112321338A
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organic fertilizer
parts
agricultural
cfu
bio
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冯艳忠
刘娣
何鑫淼
陈赫书
王文涛
田明
刘自广
吴赛辉
于晓龙
张海峰
何海娟
亓美玉
王欣
郭振华
孟详仁
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Institute of Animal Husbandry of Heilongjiang Academy of Agricultural Sciences
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Institute of Animal Husbandry of Heilongjiang Academy of Agricultural Sciences
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Fertilizers (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses a biological organic fertilizer which is mainly prepared from agricultural and forestry waste, lactic acid bacteria, saccharomycetes and bacillus. The raw materials comprise the following components in parts by weight: 800 portions of forestry and agricultural residues, 0.05 to 6.5 portions of lactobacillus powder, 0.05 to 6 portions of saccharomycete powder and 0.01 to 2.5 portions of spore bacillus powder. Mixing the raw materials, adjusting the water content to 20-85%, and then carrying out facultative fermentation at the temperature higher than 4 ℃ for 50-120 days to obtain the bio-organic fertilizer. The invention can convert organic matters in the agricultural and forestry wastes and animal manure into organic fertilizer, realizes the recycling of the agricultural and forestry wastes and the animal manure, lightens the environmental pollution, and can promote the growth of crops and improve the yield of the crops by applying the biological organic fertilizer.

Description

Biological organic fertilizer
Technical Field
The invention belongs to the technical field of fertilizers, and particularly relates to a biological organic fertilizer.
Background
The rapid development of modern agriculture and forestry in China provides abundant agricultural products and forest products for the life of people. But also produces a great deal of agricultural and forestry waste, such as straws, rice hulls, edible fungus matrixes, leftover materials, firewood, barks, peanut shells, branches, barks, wood shavings and the like. In addition, with the rapid development of the breeding industry, a large amount of waste such as animal waste is correspondingly generated. If the wastes can not be timely recycled, the serious environmental pollution problem can be caused, and the sustainable development of the society and the economy is not facilitated.
Disclosure of Invention
The invention aims to provide a biological organic fertilizer, which converts organic matters in agricultural and forestry wastes and animal manure into an organic fertilizer, realizes the recycling of the agricultural and forestry wastes and the animal manure, and reduces the environmental pollution.
The invention provides a biological organic fertilizer which is mainly prepared from agricultural and forestry waste, lactic acid bacteria, saccharomycetes and bacillus.
Preferably, the bio-organic fertilizer is prepared by mixing the raw materials, adjusting the moisture content to 20-85%, and then performing facultative fermentation at a temperature higher than 4 ℃ for 50-120 days.
Preferably, the raw materials mainly comprise, by weight, 800-1000 parts of the agricultural and forestry waste, 0.05-6.5 parts of lactobacillus powder, 0.05-6 parts of saccharomycete powder and 0.01-2.5 parts of spore bacillus powder.
Preferably, the raw materials mainly comprise 900 parts of the agricultural and forestry waste, 4.7 parts of the lactic acid bacteria powder, 0.05 part of the saccharomycete powder and 2.5 parts of the spore bacteria powder by weight.
Preferably, the lactic acid bacteria include lactobacillus casei and lactobacillus plantarum, the yeasts include saccharomyces cerevisiae and issatchenkia orientalis, and the spores include bacillus subtilis and clostridium butyricum.
Preferably, the bacterium content of the lactobacillus casei and the lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, the bacteria content of the saccharomyces cerevisiae and the Issatchenkia orientalis is more than or equal to 5.0 multiplied by 109cfu/g, the bacterium content of the bacillus subtilis and the clostridium butyricum is more than or equal to 1.0 multiplied by 1010cfu/g。
Preferably, the feedstock also includes animal manure.
Preferably, the raw material further comprises animal manure, and the animal manure is 10-5000 parts by weight.
Preferably, the feedstock also includes nitrogen.
Preferably, the raw material also comprises nitrogen, and the nitrogen accounts for 1-20 parts by weight.
The biological organic fertilizer can convert organic matters in agricultural and forestry wastes and animal manure into the organic fertilizer, realizes the recycling of the agricultural and forestry wastes and the animal manure, reduces the environmental pollution, can promote the growth of crops and improves the yield of the crops by applying the biological organic fertilizer.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The invention prepares the biological organic fertilizer by fermenting agricultural and forestry wastes, animal wastes and the like by using lactic acid bacteria, saccharomycetes and bacillus. The organic matters in the agricultural and forestry wastes and the animal manure are converted into the organic fertilizer, so that the recycling of the agricultural and forestry wastes and the animal manure is realized, the environmental pollution is reduced, the growth of crops can be promoted by applying the biological organic fertilizer, and the yield of the crops is increased.
In the first embodiment of the invention, the bio-organic fertilizer is prepared by adopting raw materials consisting of agricultural and forestry waste, lactic acid bacteria, saccharomycetes and bacillus. The specific preparation process comprises the following steps: step 1, pretreating raw materials; step 2, preparing a mixed material; and step 3, carrying out facultative fermentation on the mixed material.
In the step 1 of raw material pretreatment, agricultural and forestry waste is selected, and the agricultural and forestry waste is preferably selected from any one or a combination of two or more of crop straws, fungus dregs, waste branches and leaves of tree pruning and vines. In order to make the fermentation sufficient, the agricultural and forestry waste is mechanically crushed to the length of 3cm-5 cm.
In the step 2 of preparing the mixed material, various raw material components are weighed according to a preset weight ratio, then all the raw materials are stirred and mixed, and meanwhile, clear water is added into the mixed material to adjust the water content of the mixed material. The weight ratio of each component in the raw materials is respectively optimized as follows: 800 portions of forestry and agricultural residues, 0.05 to 6.5 portions of lactobacillus powder, 0.05 to 6 portions of saccharomycete powder and 0.01 to 2.5 portions of spore bacillus powder. The water content of the mixed materials is preferably 20-85%.
In the step 3 of carrying out facultative fermentation on the mixed materials, the initial pH of the materials is natural, the facultative fermentation is carried out at the temperature higher than 4 ℃, and the products after the fermentation are used as the bio-organic fertilizer. The fermentation mode can adopt any one of a pool method, a haystack method, a strip-stack method, a sunlight room method, a fermentation bed method and a stacking method in the prior art. Since the fermentation process is affected by the kind of raw material, fermentation mode, external environmental conditions (such as temperature change), etc., the specific fermentation period is determined according to the actual fermentation condition, preferably 50-120 days.
In the preparation process of the biological organic fertilizer, the lactic acid bacteria comprise lactobacillus casei and lactobacillus plantarum, and the bacteria content of the two lactic acid bacteria is more than or equal to 1.0 multiplied by 1010cfu/g; the yeast comprises Saccharomyces cerevisiae and Issatchenkia orientalis, and the bacteria content of both yeast is not less than 5.0 × 109cfu/g; the bacillus comprises Bacillus subtilis and Clostridium butyricum, and the bacterial content of both the bacillus and the clostridium butyricum is more than or equal to 1.0 multiplied by 1010cfu/g。
The strains can be prepared by themselves, and lactobacillus casei, lactobacillus plantarum, saccharomyces cerevisiae, issatchenkia orientalis, bacillus subtilis and clostridium butyricum are fermented and cultured in corresponding liquid culture media to obtain bacterial liquids. Then separating thalli from the fermentation culture, drying and concentrating to prepare single-strain solid bacterial powder, wherein the bacterial content of each single-strain bacterial powder is respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
In a second embodiment of the invention, the bio-organic fertilizer is prepared by adopting raw materials consisting of agricultural and forestry waste, lactic acid bacteria, saccharomycetes, bacillus and animal wastes. The preparation process is similar to that of the bio-organic fertilizer in the first embodiment, except that 10-5000 parts by weight of animal wastes are additionally added into the raw materials.
In a third embodiment of the invention, the bio-organic fertilizer is prepared by adopting raw materials consisting of agricultural and forestry waste, lactic acid bacteria, saccharomycetes, bacillus and nitrogen. The preparation process is similar to that of the biological organic fertilizer in the first embodiment, except that 1-20 parts by weight of nitrogen is additionally added into the raw materials. The nitrogen is preferably provided by nitrogen fertilizer and/or animal excrement, wherein the nitrogen fertilizer is preferably one or a combination of two or more of urea, ammonia water, ammonium bicarbonate, ammonium nitrate and ammonium sulfate.
To help better understand the technical scheme of the present invention, the following examples are provided for illustrating the bio-organic fertilizer and the preparation process thereof according to the present invention.
Example one
The raw materials of the bio-organic fertilizer comprise, by weight, 800 parts of agricultural and forestry waste, 0.05 part of lactobacillus powder, 6 parts of saccharomycete powder and 1.5 parts of spore-bacillus powder. Wherein, the lactobacillus comprises 0.02 part of lactobacillus casei and 0.03 part of lactobacillus plantarum, the microzyme comprises 4 parts of saccharomyces cerevisiae and 2 parts of issatchenkia orientalis, and the spore bacterium comprises 0.5 part of bacillus subtilis and 1 part of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes and bacillus together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 20%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 50 days by adopting a sunlight room method and aerobic fermentation at the temperature higher than 4 ℃, and a product after the fermentation is finished is used as the bio-organic fertilizer 1.
Example two
The raw materials of the bio-organic fertilizer comprise, by weight, 900 parts of agricultural and forestry waste, 4.7 parts of lactobacillus powder, 0.05 part of saccharomycete powder and 2.5 parts of spore bacillus powder. Wherein, the lactobacillus comprises 2.3 parts of lactobacillus casei and 2.4 parts of lactobacillus plantarum, the microzyme comprises 0.03 part of saccharomyces cerevisiae and 0.02 part of issatchenkia orientalis, and the spore bacterium comprises 1 part of bacillus subtilis and 1.5 parts of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes and bacillus together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 63%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the material is subjected to aerobic fermentation for 85 days by a strip-pile method at the temperature higher than 4 ℃, and a product obtained after the fermentation is finished is used as the bio-organic fertilizer 2.
EXAMPLE III
The raw materials of the bio-organic fertilizer comprise, by weight, 1000 parts of agricultural and forestry waste, 6.5 parts of lactobacillus powder, 4.7 parts of saccharomycete powder and 0.01 part of spore bacteria powder. Wherein, the lactobacillus comprises 4.3 parts of lactobacillus casei and 2.2 parts of lactobacillus plantarum, the microzyme comprises 2.3 parts of saccharomyces cerevisiae and 2.4 parts of issatchenkia orientalis, and the spore bacterium comprises 0.003 part of bacillus subtilis and 0.007 part of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes and bacillus together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 85%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 120 days by adopting a stacking method and aerobic fermentation at the temperature higher than 4 ℃, and a product obtained after the fermentation is finished is used as a biological organic fertilizer 3.
Example four
The raw materials of the bio-organic fertilizer comprise, by weight, 800 parts of agricultural and forestry waste, 0.05 part of lactobacillus powder, 6 parts of saccharomycete powder, 1.2 parts of spore-bacillus powder and 10 parts of animal waste. Wherein, the lactobacillus comprises 0.03 part of lactobacillus casei and 0.02 part of lactobacillus plantarum, the microzyme comprises 3.5 parts of saccharomyces cerevisiae and 2.5 parts of issatchenkia orientalis, and the spore bacterium comprises 0.3 part of bacillus subtilis and 0.9 part of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes, spore bacteria and animal manure together, stirring and mixing, and adding clear water while mixing and stirring to adjust the water content of the mixed material to 20%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 50 days by adopting a sunlight room method and aerobic fermentation at the temperature higher than 4 ℃, and a product after the fermentation is finished is used as a bio-organic fertilizer 4.
EXAMPLE five
The raw materials of the bio-organic fertilizer comprise 940 parts of agricultural and forestry waste, 5.3 parts of lactobacillus powder, 0.05 part of saccharomycete powder, 2.5 parts of spore bacillus powder and 3000 parts of animal waste by weight. Wherein, the lactobacillus comprises 3.0 parts of lactobacillus casei and 2.3 parts of lactobacillus plantarum, the microzyme comprises 0.04 part of saccharomyces cerevisiae and 0.01 part of issatchenkia orientalis, and the spore bacterium comprises 0.8 part of bacillus subtilis and 1.7 parts of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes, spore bacteria and animal manure together, stirring and mixing, and adding clear water while mixing and stirring to adjust the water content of the mixed material to 55%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 90 days by adopting a fermentation bed method and aerobic fermentation at the temperature higher than 4 ℃, and a product after the fermentation is finished is used as a bio-organic fertilizer 5.
EXAMPLE six
The raw materials of the bio-organic fertilizer comprise, by weight, 1000 parts of agricultural and forestry waste, 6.5 parts of lactobacillus powder, 5.5 parts of saccharomycete powder, 0.01 part of spore bacteria powder and 5000 parts of animal waste. Wherein the lactobacillus comprises lactobacillus casei 4.0 parts and lactobacillus plantarum 2.5 parts, and the yeast comprises Saccharomyces cerevisiae 3.4 parts
And Issatchenkia orientalis 2.1 parts, and the spore bacteria comprise 0.002 part of bacillus subtilis and 0.008 part of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes, spore bacteria and animal manure together, stirring and mixing, and adding clear water while mixing and stirring to adjust the water content of the mixed material to 85%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 120 days by adopting a strip-stacking method and aerobic fermentation at the temperature higher than 4 ℃, and a product obtained after the fermentation is finished is used as a bio-organic fertilizer 6.
EXAMPLE seven
The raw materials of the bio-organic fertilizer comprise, by weight, 800 parts of agricultural and forestry waste, 3.5 parts of lactobacillus powder, 6 parts of saccharomycete powder, 1.8 parts of spore-bacillus powder and 1 part of nitrogen. Wherein, the lactobacillus comprises 1.9 parts of lactobacillus casei and 1.6 parts of lactobacillus plantarum, the microzyme comprises 3.7 parts of saccharomyces cerevisiae and 2.3 parts of issatchenkia orientalis, and the spore bacterium comprises 0.4 part of bacillus subtilis and 1.4 parts of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes, bacillus and nitrogen together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 20%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 50 days by adopting a pit-and-pond method at the temperature higher than 4 ℃, and a product obtained after the fermentation is finished is used as a bio-organic fertilizer 7.
Example eight
The raw materials of the bio-organic fertilizer comprise, by weight, 880 parts of agricultural and forestry waste, 0.05 part of lactobacillus powder, 5.7 parts of saccharomycete powder, 2.5 parts of spore-bacillus powder and 13 parts of nitrogen. Wherein, the lactobacillus comprises 0.15 part of lactobacillus casei and 0.35 part of lactobacillus plantarum, the microzyme comprises 4.7 parts of saccharomyces cerevisiae and 1.0 part of issatchenkia orientalis, and the spore bacterium comprises 1.1 part of bacillus subtilis and 1.4 parts of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes, bacillus and nitrogen together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 60%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 80 days by adopting a haystack method and aerobic fermentation at the temperature higher than 4 ℃, and a product obtained after the fermentation is finished is used as a bio-organic fertilizer 8.
Example nine
The raw materials of the bio-organic fertilizer comprise, by weight, 1000 parts of agricultural and forestry waste, 6.5 parts of lactobacillus powder, 0.05 part of saccharomycete powder, 0.01 part of spore bacteria powder and 20 parts of nitrogen. Wherein, the lactobacillus comprises 3.0 parts of lactobacillus casei and 3.5 parts of lactobacillus plantarum, the microzyme comprises 0.02 part of saccharomyces cerevisiae and 0.03 part of issatchenkia orientalis, and the bacillus comprises 0.004 part of bacillus subtilis and 0.006 part of clostridium butyricum. The bacterial contents of various bacteria are respectively as follows: lactobacillus casei is greater than or equal to 1.0 x 1010cfu/g, lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, Saccharomyces cerevisiae ≥ 5.0 × 109cfu/g, Issatchenkia orientalis more than or equal to 5.0 multiplied by 109cfu/g, bacillus subtilis is more than or equal to 1.0 multiplied by 1010cfu/g, clostridium butyricum ≥ 1.0 × 1010cfu/g。
The biological organic fertilizer is prepared by the following steps.
Step 1, pretreating raw materials, namely crushing agricultural and forestry waste into particles with the length of 3-5 cm.
And 2, mixing the raw materials to prepare a mixed material, adding the crushed agricultural and forestry waste particles, lactic acid bacteria, saccharomycetes, bacillus and nitrogen together, stirring and mixing, and adding clear water while stirring to adjust the water content of the mixed material to 85%.
And 3, a fermentation step, wherein the initial pH of the material is natural, the fermentation is carried out for 120 days by adopting a strip-stacking method and aerobic fermentation at the temperature higher than 4 ℃, and a product obtained after the fermentation is finished is used as a bio-organic fertilizer 9.
In order to help better understand the technical scheme of the invention, a test example of soybean planting is provided below for illustrating the application effect of the invention.
The first test example: effect of bioorganic fertilizer on Soybean growth
A165-day soybean planting test is carried out on a bridge in Liaoning province, the same field is selected, the basic physicochemical property of the field soil is that the pH value is 6.4, the organic matter content of the plough layer soil is 12.7g/kg, and the total nitrogen is containedThe amount of the phosphorus is 1.12g/kg, the total phosphorus amount is 0.75g/kg, the total potassium amount is 18.30g/kg, the quick-acting phosphorus amount is 6.0mg/kg, the quick-acting potassium amount is 85.03mg/kg, and the alkaline hydrolysis nitrogen amount is 87.23 mg/kg. 10 groups of test designs, 9 test groups and 1 control group are designed, each group of test designs comprises 3 test cells, and the area of each test cell is 15m2All test cells are randomly distributed.
The experimental planting variety is Tiefeng 30, soybeans are sown in a direct seeding mode in late 4 months, 2 grains are planted in each hole, and about 250 seedlings are left in each cell. The test group adopts a hole application mode, the control group does not apply the bio-organic fertilizer, and the test group is according to 3000kg/hm2The bio-organic fertilizer prepared by the invention is applied in the dosage. 125 g of urea is applied to each cell in the flowering period, and watering and spraying agents for preventing and treating plant diseases and insect pests are properly carried out according to the growth condition. The soybeans in each plot were harvested and the yield was counted over 10 months.
The growth condition of the soybean is observed and recorded from 5 days after the plant field planting, and the observation is carried out once every 15 days. After the soybeans are harvested, 10 soybeans are randomly selected from each cell for counting the plant height and the pod number, meanwhile, the weight per hundred grains and the cell yield of the soybeans in each cell are counted, and the average plant height, the average pod number, the average weight per hundred grains and the average cell yield of each group of soybeans are calculated, and the results are shown in table 1.
TABLE 1
Figure BDA0002697454590000091
As can be seen from the data in Table 1, the 9 groups of soybeans applied with the bio-organic fertilizer are obviously higher than the control group in four properties of plant height, pod number, hundred grain weight and cell yield. Therefore, the prepared bio-organic fertilizer 1-9 can obviously promote the growth of soybeans and improve the yield of the soybeans.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.

Claims (10)

1. A biological organic fertilizer is mainly prepared from agricultural and forestry waste, lactic acid bacteria, saccharomycetes and bacillus.
2. The bio-organic fertilizer according to claim 1, characterized in that: the bio-organic fertilizer is prepared by mixing the raw materials, adjusting the water content to be 20-85%, and then carrying out facultative fermentation at the temperature higher than 4 ℃ for 50-120 days.
3. The bio-organic fertilizer according to claim 1, characterized in that: the raw materials mainly comprise, by weight, 800-1000 parts of agricultural and forestry waste, 0.05-6.5 parts of lactobacillus powder, 0.05-6 parts of saccharomycete powder and 0.01-2.5 parts of spore bacillus powder.
4. The bio-organic fertilizer according to claim 3, characterized in that: the raw materials mainly comprise 900 parts of agricultural and forestry waste, 4.7 parts of lactobacillus powder, 0.05 part of saccharomycete powder and 2.5 parts of spore bacillus powder by weight.
5. Bio-organic fertilizer according to any one of claims 1 to 4, characterized in that: the lactobacillus comprises lactobacillus casei and lactobacillus plantarum, the yeast comprises saccharomyces cerevisiae and issatchenkia orientalis, and the bacillus comprises bacillus subtilis and clostridium butyricum.
6. The bio-organic fertilizer according to claim 5, characterized in that: the bacteria content of the lactobacillus casei and the lactobacillus plantarum is more than or equal to 1.0 multiplied by 1010cfu/g, the bacteria content of the saccharomyces cerevisiae and the Issatchenkia orientalis is more than or equal to 5.0 multiplied by 109cfu/g, the bacterium content of the bacillus subtilis and the clostridium butyricum is more than or equal to 1.0 multiplied by 1010cfu/g。
7. Bioorganic fertilizer according to claim 1 or 2, characterized in that: the raw material also comprises animal waste.
8. The bio-organic fertilizer according to claim 3, characterized in that: the raw material also comprises animal manure, and the animal manure is 10-5000 parts by weight.
9. Bioorganic fertilizer according to claim 1 or 2, characterized in that: the raw material also comprises nitrogen.
10. The bio-organic fertilizer according to claim 3, characterized in that: the raw material also comprises nitrogen, and the weight of the nitrogen is 1-20 parts.
CN202011010650.6A 2020-09-23 2020-09-23 Biological organic fertilizer Pending CN112321338A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112500216A (en) * 2020-11-02 2021-03-16 东北农业大学 Bio-organic fertilizer with crop rooting promoting function and preparation method thereof
CN113264786A (en) * 2021-06-30 2021-08-17 上海为绿景观建设有限公司 Fertilizer prepared by fermenting agricultural and forestry wastes and fermentation process thereof
CN113604380A (en) * 2021-07-15 2021-11-05 山东益昊生物科技有限公司 Method for producing composite microbial inoculum by fermenting clostridium butyricum centrifugal waste liquid, corresponding composite microbial inoculum and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105152705A (en) * 2015-08-28 2015-12-16 安徽欧杰利生态肥业有限公司 Bio-organic fertilizer based on agricultural and forestry wastes and production method of bio-organic fertilizer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105152705A (en) * 2015-08-28 2015-12-16 安徽欧杰利生态肥业有限公司 Bio-organic fertilizer based on agricultural and forestry wastes and production method of bio-organic fertilizer

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112500216A (en) * 2020-11-02 2021-03-16 东北农业大学 Bio-organic fertilizer with crop rooting promoting function and preparation method thereof
CN113264786A (en) * 2021-06-30 2021-08-17 上海为绿景观建设有限公司 Fertilizer prepared by fermenting agricultural and forestry wastes and fermentation process thereof
CN113604380A (en) * 2021-07-15 2021-11-05 山东益昊生物科技有限公司 Method for producing composite microbial inoculum by fermenting clostridium butyricum centrifugal waste liquid, corresponding composite microbial inoculum and application thereof

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Application publication date: 20210205