CN100569704C - A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate - Google Patents
A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate Download PDFInfo
- Publication number
- CN100569704C CN100569704C CNB2007100229114A CN200710022911A CN100569704C CN 100569704 C CN100569704 C CN 100569704C CN B2007100229114 A CNB2007100229114 A CN B2007100229114A CN 200710022911 A CN200710022911 A CN 200710022911A CN 100569704 C CN100569704 C CN 100569704C
- Authority
- CN
- China
- Prior art keywords
- phosphate
- bacterial strain
- organic fertilizer
- fertilizer
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Fertilizers (AREA)
Abstract
The present invention is a kind of can dissolve the bacterium K3 bacterial strain of soil calcium phosphate and the microorganism organic fertilizer of production thereof, belongs to the intensive agriculture production technology.Be separated to 1 strain from calcareous soil and have the very capable bacterium of dissolving phosphoric acid calcium, this bacterium is carried out liquid fermenting production earlier, be mixed and made into the phosphate solubilizing microorganism organic fertilizer with specific organic carrier then, contain 5 * 10 in the fertilizer
7Above phosphate solubilizing microorganism quantity, the total nitrogen content of individual/g is 4% (being organonitrogen more than 90%), full potassium content 1～2%, content of tatal phosphorus 1～2%, and organic content is 35%.Test-results shows, uses 100 kilograms of this microorganism organic fertilizers for every mu on calcareous soil, and it provides the crop effect of the plain nutrition of phosphorus to be equivalent to every mu and uses 10 kilograms of P
2O
5Chemical phosphatic ferfilizer.Laboratory culture result shows that this phosphorus bacteria can make that titanium pigment concentration reaches 643mg/L in the calcium phosphate solution, and is higher more than 4 times than the foreign latest results reported.
Description
One, technical field
The present invention is a kind of can dissolve the bacterium K3 bacterial strain of soil calcium phosphate and the microorganism organic fertilizer of making thereof, and belongs to the intensive agriculture production technical field, is exclusively used in to overcome the scarce phosphorus problem of crop on the calcareous soil.
Two, background technology
The chemical phosphatic ferfilizer back this season crop that is manured into soil generally can only utilize about 15%, and about 85% phosphate fertilizer is fixed in the soil and is progressively aging and lose validity, even if following season crop also be difficult to utilize the phosphate fertilizer that applied in last season.Therefore, the peasant has to per season and uses chemical phosphatic ferfilizer, like this, a large amount of phosphate rock resources is exhausted on the one hand, it is predicted that the existing higher-grade phosphate rock resource of China had only all to be exhausted in 30 years by present development and use speed, on the other hand, the peasant uses phosphate fertilizer and make in the soil accumulation attitude phosphorus a lot of in per season, but plant is difficult to utilize.Therefore, inventing a kind of microorganism or microorganism organic fertilizer and can dissolve unavailable phosphorus in the soil, make its effectuation, is a very important ring in the management of China's soil phosphorus and phosphate fertilizer.
Phosphorus bacteria has two kinds according to its mode of action difference in the soil, a kind of is inorganic phosphorus bacteria, a kind of is the organophosphorus bacterium, the former is the vital movement generation organic acid (as citric acid, lactic acid, oxysuccinic acid etc.) by thalline, these organic acids are by the calcium in the chelating calcareous soil and reduce pH and make calcium phosphate instability in the soil, and discharge phosphorus, thereby will in the soil invalid inorganic calcium phosphorus (except the acid soil) compound of plant be become effective inorganic phosphorus.Utilize the microorganism of some specific functions transfer in the soil unavailable phosphorus this be one of developing direction of microorganism organic fertilizer.The another kind of phosphorus bacteria fertilizer (organophosphorus bacterium) is will in the soil the invalid organo phosphorous compounds of plant to be resolved into the absorbent inorganic phosphorus of plant by the microorganism secretion Phosphoric acid esterase, and it is more obvious that this class phosphorus bacteria fertilizer applies on the organic content soil with high effect.
On the other hand, the solid organic castoff of agricultural products in China processing industry and the feces of livestock and poultry behind the large-scale cultivation have been thrown aside etc. not only severe contamination everywhere environment, also greatly having wasted can be as the raw material of fertilizer and microbial organic fertilizer product; A large amount of nutrient resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened the ability of China's agricultural sustainable development significantly.The nutritive element how will take away because of harvesting crops from soil is returned in the soil to greatest extent and goes, and unique approach is made the organic commercial fertilizer material with these solid organic castoffs exactly and is manured into soil.If these solid organic castoffs through the synthetic high-grade organic compost of thermophilic fermentation, are used further to the organic carrier of agriculture function yeast, made microorganism organic fertilizer definite functions will have good application prospects.
Three, summary of the invention
Technical problem the objective of the invention is to develop a kind ofly can dissolve the bacterium of soil calcium phosphate and the microorganism organic fertilizer of making thereof, unavailable phosphorus in the calcareous soil (calcium phosphate) is changed into part available state phosphorus, thereby improve the plain nutrition of crop phosphorus, save a large amount of phosphate rock resources, guarantee the Sustainable development of China's agriculture production.
Technical scheme
1, the physio-biochemical characteristics of phosphate-solubilizing bacteria K3 bacterial strain
The K3 bacterial strain of a kind of energy dissolving phosphoric acid calcium of the present invention, it is characterized in that, it is characterized in that this bacterial strain is Pseudomonas aeruginosa (Pseudomonas aeruginosa), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 14th, 2007, culture presevation number is CGMCC NO.2045, and main biological characteristics is G
-, thalline is shaft-like, amphimicrobian; Catalase, oxydase, indole test, clark and Lubsreaction are positive, and V.P tests negative; Can utilize gelatin, litmus milk is solidified, but can not utilize starch and Citrate trianion; Glucose, sucrose and lactose fermentation be aerogenesis not.The query ID of this K3 bacterial strain 16S rDNA in Genban is: EF064786.
2, the microorganism organic fertilizer of being produced with phosphate-solubilizing bacteria K3 bacterial strain
Contain 5 * 10 in this fertilizer
7The phosphate solubilizing microorganism quantity that individual/g is above; Total nitrogen content is 4%, wherein is organonitrogen more than 90%; Full potassium content 1～2%; Content of tatal phosphorus 1～2%; Organic content is 35%.
3, the method with K3 bacterial strain production microorganism organic fertilizer comprises:
1) select the described phosphate-solubilizing bacteria K3 of claim 1 bacterial strain for use, carry out liquid fermenting production earlier, the condition of its fermentative production is: pH growth scope 5.0～8.0,25～45 ℃ of culture temperature scopes, liquid nutrient medium is that LB adds richness, fermentation period 18～30 hours, K3 bacterial strain bacteria containing amount 〉=1 * 10 in the fermented liquid
10Individual/ml;
2) the organic carrier production of phosphate-solubilizing bacteria: " utilize agricultural solid residue to make the method and the fertilizer product thereof of organic fertilizer with high nitrogen " according to the patent that this seminar obtains, the organic fertilizer with high nitrogen that the patent No.: ZL2004 1 0014773.1 described methods are produced is an organic carrier;
3) production of phosphate-solubilizing bacteria K3 bacterial strain organic fertilizer: placed under field conditions (factors) 3-5 days after above-mentioned organic carrier being added by weight the fermented liquid of 5% phosphate-solubilizing bacteria K3 bacterial strain fermentation liquor phosphate-solubilizing bacteria and organic carrier thorough mixing, phosphate-solubilizing bacteria quantity will be from 1 * 10 therebetween
7Breeding is to 1 * 10 about individual/g
8Individual/g, contain phosphate-solubilizing bacteria quantity in the finished product and reach 5 * 10
7Individual/more than the g, water content is less than 30%, and total nitrogen content reaches 4% (organonitrogen is greater than 90%), and organic content reaches 35%, reaches the standard of national microorganism organic fertilizer product.
Phosphate solubilizing bacteria fermentative production optimum temps is 37.5 ℃ in the aforementioned production method.Substratum LB adds richness and refers to add ammonium nitrate, sulphur ammonium, analysis for soybean powder etc. in LB in its microbial fermentation production method.
Beneficial effect
A kind of microorganism organic fertilizer and the production method thereof that can dissolve soil calcium phosphate of the present invention, utilize cultural solid organic waste composting to become specific organic carrier, make microorganism organic fertilizer again with after the phosphate-solubilizing bacteria fermented liquid mixes, its product is compared with product in the market has following advantage:
1) contain the efficient bacterial strain that dissolves soil calcium phosphate in the fertilizer product, its phosphorus decomposing effect highly significant, the content of this efficient bacterial strain reaches 5 * 10 in the fertilizer
7Individual/as more than the g, to form the advantage group rapidly after being manured into soil, and under in soil, surviving.
2) contain abundant organic carbon and organonitrogen in the fertilizer product, very superior growing environment is provided for phosphorus decomposing function bacterium, greatly improved the field planting of external functional microorganism in soil and the ability that plays a role.
3) the laboratory culture result shows that this phosphorus bacteria can make that titanium pigment concentration reaches 643mg/L in the calcium phosphate solution, and is higher more than 4 times than the foreign latest results reported.
4) this products production raw material sources extensively enrich, raw materials cost is very low, production technique is easy, the fixed capital less investment, the power consumption water consumption is few in the production process, therefore the product price is low and with strong points, 1 ton of microorganism organic fertilizer that contains phosphate-solubilizing bacteria of the every production of enterprise, its all costs are no more than 600 yuan, and the microorganism organic fertilizer market value that resembles this special efficacy special use is higher, generally about 1000 yuan/ton, and very in short supply in the market, so this product has powerful society, environment and economic vaibility.
5) biological test result in land for growing field crops shows, after using this product on corn, the Soybean and Other Crops, phosphate-solubilizing bacteria is bred rapidly, formation advantage group in soil, make that available phosphorus significantly improves in the soil, use 100 kilograms of this microorganism organic fertilizers for every mu on calcareous soil, it provides the crop effect of the plain nutrition of phosphorus to be equivalent to every mu and uses 10 kilograms of (P
2O
5) chemical phosphatic ferfilizer (75 kilograms of calcium superphosphate), the chemical nitrogen fertilizer (18 kilograms of urea) of 8 kilograms (N), 1-2 kilogram (K
2O) chemical potash fertilizer (3 kilograms of Repone K).The input cost that the peasant uses this microorganism organic fertilizer is 100 yuan, and the input cost of using above-mentioned chemical fertilizer is 120 yuan.Moreover, after using microorganism organic fertilizer product of the present invention, quality of agricultural product and the local flavor produced improve a lot, and ecotope and soil quality are greatly improved.
Four, description of drawings
The efficient phosphate-solubilizing bacteria K3 of Fig. 1 strains separation screening process figure
Fig. 2 obtains efficient phosphate-solubilizing bacteria K3 bacterial strain by PVK solid medium separation screening
The liquid fermentation and culture technical process of Fig. 3 phosphate-solubilizing bacteria
Five, embodiment
(1) acquisition of phosphorus decomposing bacterial strain
Lack well-grown plant rhizosphere collection soil of selection and plant root the phosphorus soil from calcareous, sample low temperature (4-15 ℃) is preserved and is taken back the laboratory; Adopt seriation to utilize solid plate selective medium (glucose 10g, tricalcium phosphate 5g, the ammonium sulfate 0.5g of PVK improvement, sodium-chlor 0.2g, sal epsom 0.1g, Repone K 0.2g, yeast extract 0.5g, manganous sulfate 0.002g, ferrous sulfate 0.002g, 0.4% tetrabromophenol sulfonphthalein 6ml, agar 18g, distilled water 1000ml, pH nature) cultivates under 37.5 ℃ of conditions and obtained phosphate-solubilizing bacteria list bacterium colony in 36-48 hour, select the bigger bacterial strain of molten phosphorus circle and bacterium colony circle ratio further to carry out shaking table and screen; Adopt NBRIP liquid nutrient medium (glucose 10g, tricalcium phosphate 5g, magnesium chloride 5g, sal epsom 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, distilled water 1000ml is pH7.0.) with ground phosphate rock improved culture medium (glucose 10g, ground phosphate rock 5g, magnesium chloride 5g, sal epsom 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, distilled water 1000ml, pH7.0) under 37.5 degree conditions, cultivate 18-24 hour acquisition phosphate solubilizing bacteria fermented liquid, measure phosphorus content in fermented liquid and the thalline, select 4 higher strain bacterial strains of amount of phosphorus dissolved to carry out pot experiment; On the calcareous soil that lacks phosphorus, carry out pot experiment with the live mode of corn, it is in full accord that nitrogen potassium nutrition rate of fertilizer application keeps, the phosphorus nutrient is used calcium superphosphate respectively, potassium primary phosphate, the phosphate-solubilizing bacteria fermented liquid, deactivation phosphate-solubilizing bacteria fermented liquid substitutes and applies fertilizer, hot-house culture through 30-35 days, measure available phosphorus content in full phosphorus amount of plant and the soil, according to healthy and strong situation of plant strain growth and the full phosphorus amount of plant, soil available phosphorus content just screens efficient phosphorus decomposing bacterial strain, promptly obtain the K3 bacterial strain, plant definite the genus through Physiology and biochemistry and 16sRNA Molecular Identification, the bacterial classification freeze-drying is preserved in-20 ℃.
(2) fermentative production of phosphorus decomposing bacterial strain
1, the seed culture of phosphate-solubilizing bacteria K3 bacterial strain
Seed culture medium (shake-flask culture): substratum LB adds richness, 121 ℃ of steam sterilizings 20 minutes;
Seed culture condition: 5-10% inoculum size, 37.5 degree, 150-200 rev/min; Culture cycle 18-25 hour.
2, the fermentative production of bacterial strain (enlarged culturing): utilize the LB enriched medium to carry out the high-density enlarged culturing and obtain high gravity fermentation liquid (bacteria containing amount 〉=1 * 10
10Individual/ml).
Fermention medium: substratum LB adds richness (adding rich major ingredient is ammonium nitrate, sulphur ammonium and analysis for soybean powder etc.), 121 ℃ of steam sterilizings 20 minutes;
Fermentation condition: 5-10% inoculum size, 80-100 rev/min, 37.5 ℃ of culture temperature, pH7.0-7.5, fermentation period 20-25 hour.Dissolved oxygen is stabilized in 100%-60%, showing that when the dissolved oxygen bottom out and when progressively trending towards 75% fermentation enters the mild phase and can put jar, puts jar condition and is: living bacteria count 〉=1 * 10
10Individual/ml, high density fermentation bacterium liquid cryopreservation is stand-by.
3, the production of phosphate-solubilizing bacteria K3 bacterial strain biological organic fertilizer
Organic carrier is (by Chinese patent " method and the fertilizer product thereof that utilize agricultural solid residue to make ", the organic fertilizer with high nitrogen that the patent No.: ZL2004 1 0014773.1 described methods are produced is an organic carrier) preparation: with feces of livestock and poultry, crop material, inorganic nitrogenous fertilizer and bean dregs are pressed dry weight basis respectively with 75-85: 10-20: 1-8: the secondary fermentation of 5-10 mixed, treat to keep when compost temperature rises to 60-70 ℃ 3-7 days, turning in later every 2-3 days once, the inorganic nitrogenous fertilizer that at every turn adds 1-4% (dry weight percentage) equably, begin cooling until compost temperature from 50-60 ℃, further after-ripening in composting 5-8 days again, the organic fertilizer product of making is the organic fertilizer with high nitrogen of acquisition.
Phosphate-solubilizing bacteria and above-mentioned organic carrier thorough mixing (are added the phosphate-solubilizing bacteria fermented liquid by weight 5%,) mixture behind the mixing scatter and disappear under field conditions (factors) moisture to water content less than 30%, this process approximately needs 3-5 days time, and phosphate-solubilizing bacteria quantity will be from 1 * 10 therebetween
7Breeding is to 1 * 10 about individual/g
8Individual/g, contain phosphate-solubilizing bacteria quantity in the finished product and reach 5 * 10
7Individual/more than the g, total nitrogen content reaches 4% (organonitrogen is greater than 90%), and organic content reaches 35%, and product index reaches the standard of national microorganism organic fertilizer.Cool place, dry place are deposited in the packing of product.
Table 1 some characteristic of microorganism organic fertilizer product of the present invention
| Total organic matter (%) | Dissolved Organic Matter (DOM) (%) | Total organic nitrogen (%) | Full phosphorus P 2O 5 (％) | Full potassium K 2O (％) | PH (flooding) | Phosphate-solubilizing bacteria K3 viable count (individual/g) |
| 35.8 | 9.52 | 4.12 | 1.65 | 1.13 | 6.8 | 5×10 7 |
Table 1 shows that organic content is 35.8% in the product of producing by the technology of the present invention and production technique, and contain very a high proportion of Dissolved Organic Matter (DOM) (9.52%) and very high organonitrogen content (4.12%), this is the prolific basic substance of phosphorus decomposing function bacterium after this product is manured into soil; Simultaneously, also significantly promoted the biological activity of soil microorganisms after organic carbon that these are a large amount of and organonitrogen are manured into soil, thereby improved the soil nutrient conversion rate greatly, made crop growth be in good nutritional status in the phase all the time; The viable count of phosphate-solubilizing bacteria reaches 5 * 10 in the product
7Individual.Above characteristic has proved absolutely the feasibility of the extraordinary phosphate solubilizing microorganism organic fertilizer that the technology of the present invention and production technique are made.
4, the field test of phosphate-solubilizing bacteria K3 bacterial strain biological organic fertilizer
The extraordinary phosphate solubilizing microorganism organic fertilizer that the present invention developed is to the yield increasing effect highly significant (seeing Table 2) of corn on the calcareous soil, if (table 2 is handled 3 and is deducted and handle 1 divided by handling 1 according to high by 27.1% only to use organic fertilizer with high nitrogen (organic carrier) maize grain yield comparison as shown in Table 2 on calcareous soil, multiply by 100% again), illustrate that this fertilizer has certain production-increasing function to corn yield.If only use phosphate-solubilizing bacteria liquid, the maize grain yield comparison is shone only high by 5.7% (table 2 is handled 4 and deducted processing 1 divided by handling 1, multiply by 100% again), illustrates that using the phosphate-solubilizing bacteria fermented liq uses the effect of increasing production of corn not remarkable separately.If use microorganism organic fertilizer (handling 5), then maize grain yield approaches local peasant's conventional fertilizer application output, and comparison is according to high by 43.2%.If use behind the microorganism organic fertilizer at a small amount of urea of compounding application and Repone K, then corn yield is the highest for all processing, (table 2 is handled 6 and is deducted processing 2 divided by handling 2 also to increase production 3.9% than local peasant's conventional fertilizer application, multiply by 100% again), by handle 6 with handle 2 relatively, draw every mu and use 100 kilograms of this microorganism organic fertilizers, it provides the crop effect of the plain nutrition of phosphorus to be equivalent to every mu and uses 75 kilograms of calcium superphosphate, 18 kilograms of urea and 3 kilograms of Repone K), the input cost that the peasant uses this microorganism organic fertilizer is 100 yuan, and the input cost of using above-mentioned chemical fertilizer is 120 yuan.
Table 2 phosphate solubilizing microorganism organic fertilizer of the present invention is to the yield increasing effect (field test) of corn on the calcareous soil
| Handle numbering | Handle and describe | Maize grain yield (kg/ mu) | Maize Absorption total phosphorus content (kg/ mu) |
| 1 | CK | 458.3±26.9 | 16.9±4.4 |
| 2 | Local peasant's conventional fertilizer application: urea 45kg/ mu+calcium superphosphate 75kg/ mu+Repone K 20kg/ mu | 692.8±32.4 | 25.3±3.9 |
| 3 | Use organic carrier (100kg/ mu) | 582.7±24.7 | 20.6±3.2 |
| 4 | Use phosphate-solubilizing bacteria liquid (using 5kg phosphate-solubilizing bacteria fermented liq for every mu) | 484.6±46.5 | 19.6±4.5 |
| 5 | Use microorganism organic fertilizer (100kg/ mu): organic carrier+phosphate-solubilizing bacteria liquid | 656.3±37.1 | 22.0±3.7 |
| 6 | Use microorganism organic fertilizer (100kg/ mu): organic carrier+phosphate-solubilizing bacteria liquid+urea 27kg/ mu+Repone K 17kg/ mu | 719.6±26.9 | 26.3±2.8 |
Annotate: all organic fertilizer, phosphate fertilizer and potash fertilizer is all disposable to be made base manure and is manured into soil, and urea divides and is manured into soil that (base manure 60% topdresses 20% for the first time, topdresses 20% for the second time for three times.)
Also find out from table 2, use that the total phosphorus content of Maize Absorption significantly increases behind this microorganism organic fertilizer, because this soil has soil total phosphorous height and the feature of available phosphorus very low (4.6mg/kg), use and accelerated the conversion rate that the soil unavailable phosphorus changes into available state phosphorus behind this microorganism organic fertilizer, corn systemic total phosphorus content in the time of infertility is significantly improved, thereby the plain nutrition of the phosphorus of having guaranteed corn makes the corn with high yield stable yields.
Can find out from table 3, use the phosphate solubilizing microorganism organic fertilizer, through one season corn growth, the quantity of phosphate-solubilizing bacteria significantly increases in this soil, reaches 6 * 10
4Individual/g soil, illustrate that this phosphate solubilizing microorganism organic fertilizer can make external phosphate-solubilizing bacteria field planting and breeding in soil really, and play a role, behind the one season corn in the soil available phosphorus be 7.02mg/kg, and below 1,2, the 3 processing soil available phosphorus 3.5mg/kg, only, illustrate that the phosphorus decomposing effect of this microorganism organic fertilizer is remarkable for using half that the phosphate solubilizing microorganism organic fertilizer is handled.
Table 3 phosphate solubilizing microorganism organic fertilizer of the present invention is to the influence (pedotheque when pot experiment finishes) of phosphate-solubilizing bacteria on the calcareous soil and soil phosphorus content
| Handle numbering | Handle and describe | Phosphate-solubilizing bacteria (individual/g soil) | Available phosphorus content (mg/kg) | Total phosphorous (%) |
| 1 | CK | 2×10 1 | 2.89 | 0.143 |
| 2 | Use organic carrier (100kg/ mu) | 3×10 1 | 345 | 0.149 |
| 3 | Use phosphate-solubilizing bacteria liquid | 7×10 2 | 3.50 | 0.141 |
| 4 | Use microorganism organic fertilizer (100kg/ mu): organic carrier+phosphate-solubilizing bacteria liquid | 6×10 4 | 7.02 | 0.136 |
Annotate: all fertilizer are all disposable to be made base manure and is manured into soil, and trial crops is a corn.
Claims (6)
1, a kind of bacterium K3 bacterial strain of energy dissolving phosphoric acid calcium, it is characterized in that, this bacterial strain is Pseudomonas aeruginosa (Pseudomonas aeruginosa), is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on May 14th, 2007, and culture presevation number is CGMCC NO.2045.
2, the microorganism organic fertilizer of being produced with the described a kind of bacterium K3 bacterial strain that can dissolve soil calcium phosphate of claim 1.
3, according to the described microorganism organic fertilizer that can dissolve soil calcium phosphate of claim 2, it is characterized in that: contain 5 * 10 in this fertilizer
7The phosphate solubilizing microorganism quantity that individual/g is above; Total nitrogen content is 4%, wherein is organonitrogen more than 90%; Full potassium content 1～2%; Content of tatal phosphorus 1～2%; Organic content is 35%.
4, according to claim 2 or the 3 described microorganism organic fertilizers that can dissolve soil calcium phosphate, it is characterized in that, obtain by following production method:
1) phosphate-solubilizing bacteria K3 bacterial strain fermentation liquor is produced: select the described phosphate-solubilizing bacteria K3 of claim 1 bacterial strain for use, carry out liquid fermenting production, the condition of its fermentative production is: pH growth scope 5.0～8.0,25～45 ℃ of culture temperature scopes, liquid nutrient medium is that LB adds richness, fermentation period 18～30 hours, K3 bacterial strain bacteria containing amount 〉=1 * 10 in the fermented liquid that is obtained
10Individual/ml;
2) the organic carrier production of phosphate-solubilizing bacteria: " utilize agricultural solid residue to make the method and the fertilizer product thereof of organic fertilizer with high nitrogen " according to Chinese patent, the organic fertilizer with high nitrogen that the patent No.: ZL2004 1 0014773.1 described methods are produced is an organic carrier;
3) production of phosphate-solubilizing bacteria K3 bacterial strain organic fertilizer: above-mentioned organic carrier is added 5% phosphate-solubilizing bacteria K3 bacterial strain fermentation liquor by weight, behind the thorough mixing, placed under field conditions (factors) 3-5 days, be the phosphate solubilizing microorganism organic fertilizer product of acquisition.
5, according to the described phosphate solubilizing microorganism organic fertilizer of claim 4, phosphate solubilizing bacteria fermentative production optimum temps is 37.5 ℃ in its production method.
6, according to claim 4 or 5 described phosphate solubilizing microorganism organic fertilizer, substratum LB adds richness and refers to add ammonium nitrate, sulphur ammonium and analysis for soybean powder in LB in its microbial liquid fermentation method for producing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100229114A CN100569704C (en) | 2007-05-25 | 2007-05-25 | A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100229114A CN100569704C (en) | 2007-05-25 | 2007-05-25 | A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101066897A CN101066897A (en) | 2007-11-07 |
| CN100569704C true CN100569704C (en) | 2009-12-16 |
Family
ID=38879618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100229114A Active CN100569704C (en) | 2007-05-25 | 2007-05-25 | A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100569704C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2453596C1 (en) * | 2010-12-29 | 2012-06-20 | Государственное научное учреждение Татарский научно-исследовательский институт агрохимии и почвоведения Российской академии сельскохозяйственных наук (ГНУ Татарский НИИАХП Россельхозакадемии) | Strain of pseudomonas brassicacearum used to obtain bacterial fertiliser for grain, vegetable and forage crops |
| ES2730111T3 (en) | 2011-02-07 | 2019-11-08 | Fertinagro Biotech Sl | Method of obtaining a biological fertilizer from apatite minerals through microorganisms and biological fertilizer thus obtained |
| CN102604860B (en) * | 2012-02-07 | 2013-05-08 | 南京林业大学 | Burkholderia multivorans WS FJ9 and application thereof to growth promotion of pine |
| CN102690763B (en) * | 2012-02-23 | 2014-01-01 | 西北大学 | Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof |
| CN103146610B (en) * | 2013-03-12 | 2014-08-20 | 南京农业大学 | Plant growth-promoting rhizobacteria and application thereof |
| CN105062910B (en) * | 2015-07-16 | 2018-04-03 | 南京林业大学 | One plant of willow rhizosphere phosphate-solubilizing bacteria Pseudomonas frederiksbergensis JW SD2 and its application |
| US20220033320A1 (en) | 2018-10-05 | 2022-02-03 | Fertinagro Biotech, S.L. | Method for obtaining a granulated phosphate fertiliser and phosphate fertiliser obtained |
| CN110702842A (en) * | 2019-09-26 | 2020-01-17 | 广西壮族自治区林业科学研究院 | Method for monitoring fertilizer loss rate |
| CN110616179B (en) * | 2019-11-13 | 2023-04-07 | 东莞市农业科学研究中心 | Pseudomonas aeruginosa DGNK-JL2 and application thereof |
-
2007
- 2007-05-25 CN CNB2007100229114A patent/CN100569704C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101066897A (en) | 2007-11-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100569704C (en) | A kind of K3 bacterial strain and microorganism organic fertilizer thereof that can dissolve soil calcium phosphate | |
| CN102992827B (en) | Active organic bacterial fertilizer and preparation method thereof | |
| CN103011961B (en) | Active organic bacterial manure and preparation method thereof | |
| CN101209933B (en) | Refined biological organic fertilizer and its preparing process | |
| CN104045469B (en) | A kind of biological organic fertilizer being exclusively used in saltings and its preparation method and application | |
| CN101463334B (en) | Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof | |
| CN1618772A (en) | High efficiency biological organic fertilizer and its production technology | |
| CN101239847B (en) | Liquid composite microbial fertilizer and its preparation method | |
| CN102701834A (en) | Biomass charcoal based probiotic preparation and preparation method thereof | |
| CN103232944A (en) | Microorganism bacterium agent for straw and excrement mixed composting | |
| CN100497267C (en) | Process of fermenting fruit dregs and stalk to produce fulvic acid bacterial manure | |
| CN104003804A (en) | Moisture-retaining and nitrogen-fixing compound microbial fertilizer and preparation method thereof | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN102951966A (en) | Production method for compound microbial manure | |
| CN101760202A (en) | Compound biological soil improver and preparation method thereof | |
| CN108863658A (en) | A kind of charcoal base organic fertilizer and preparation method | |
| CN107285925A (en) | A kind of method of utilization microorganism formulation stalk stack retting returning to the field | |
| CN112645746A (en) | Animal manure straw bio-organic fertilizer and preparation method thereof | |
| CN106699474A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| CN101648827A (en) | Fermented biological organic fertilizer and processing method thereof | |
| CN112062616A (en) | Decomposition agent for promoting in-situ returning of crop straws and application thereof | |
| CN111393206A (en) | Organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof | |
| CN102219575B (en) | Industrial method for producing bio-fertilizer inoculant by utilizing decayed cow dung as raw material | |
| CN103409351A (en) | Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same | |
| CN104817372A (en) | Special biological phosphorus fertilizer for cucumber, and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180810 Address after: 214200 Gaosheng town industrial agglomeration area, Yixing City, Wuxi, Jiangsu (Xu Jing Village) Patentee after: JIANGSU PENGYAO ENVIRONMENTAL ENGINEERING CONTRACTING CO., LTD. Address before: Nanjing City, Jiangsu province 210095 Weigang No. 1 Nanjing Agricultural University Department of science and technology Qian Baoying Patentee before: Nanjing Agricultural University |