CN111393206A - Organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof - Google Patents
Organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
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- C—CHEMISTRY; METALLURGY
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Abstract
The invention discloses a composite bacteria-containing organic-inorganic biological complete-element bio-organic fertilizer and application thereof, wherein the composite bacteria-containing complete-element bio-organic fertilizer is prepared by mixing single-strain bio-organic fertilizers according to the ratio of 1:1:1 and then adding urea, calcium superphosphate and potassium sulfate in different proportions, the single-strain bio-organic fertilizer is prepared by adding acid-hydrolyzed amino acid into common organic fertilizers to increase exogenous nutrition and adjust pH, and then inoculating functional bacteria to perform solid fermentation, and the composite bacteria-containing complete-element bio-organic fertilizer contains more than or equal to 2 × 10 functional bacteria7CFU/g, the total nutrient of nitrogen, phosphorus and potassium is more than or equal to 8.0 percent, the mass fraction of organic matters (calculated by dry basis) is more than or equal to 30 percent, the pH value is 5.5-7.5, and the water content is less than or equal to 30 percent. The product can effectively promote the growth of corn and Chinese cabbage, improve the yield, has good growth promoting effect, and can also effectively increase the crop yield after replacing part of chemical fertilizers.
Description
Technical Field
The invention belongs to the field of biological fertilizers, and particularly relates to an organic-inorganic full-element biological organic fertilizer containing composite bacteria and application thereof.
Background
At present, 5900 million tons of nutrient resources are consumed by chemical fertilizers every year in China, which accounts for about 30 percent of the total consumption of the chemical fertilizers in the world, the utilization rate of chemical nitrogen fertilizers is only 30 percent, the loss rate is up to 60 percent, nonrenewable resources (coal and natural gas) for chemical fertilizer production are greatly consumed, and a series of outstanding problems of serious damage to soil structures, rapid reduction of organic matter content, serious unbalance of soil microbial community structures and the like are caused by long-term over-application of the chemical fertilizers and especially the chemical nitrogen fertilizers. Therefore, it is very important to develop new fertilizers to enhance soil activity and to improve crop yield sustainably. On the other hand, a large amount of livestock and poultry manure, crop straws, agricultural product processing waste, kitchen wastes, town sludge and the like are generated in China every year, and if the livestock and poultry manure, the crop straws, the agricultural product processing waste, the kitchen wastes, the town sludge and the like are not well utilized, the environment of China is polluted. How to research and develop novel fertilizers and reasonably apply the fertilizers becomes an important subject for agricultural development in China. However, the traditional organic fertilizer has low content of available nutrients, slow fertilizer efficiency, large application amount, large consumption of manpower and material resources in the manufacturing and application processes and low economic benefit. The biological fertilizer can improve soil fertility, stimulate and regulate crop growth, strengthen resistance and disease resistance and raise crop quality through the action of great amount of beneficial microbes. But because the fertilizer does not contain nutrient elements, the fertilizer is an indirect fertilizer and cannot completely replace chemical fertilizers and organic fertilizers. Therefore, the development of a novel fertilizer production technology which integrates quick fertilizer efficiency, long organic fertilizer efficiency and biological fertilizer efficiency improvement into a whole and the industrialization popularization and long-term use of the novel fertilizer production technology are urgently needed, and the purposes of improving the yield per unit, improving the soil structure, greatly improving the organic matter content of cultivated land and constructing a healthy and high-yield soil microbial community are further achieved. The novel all-element bio-organic fertilizer has the functions of activating soil and converting soil nutrients of biological bacteria, and has the characteristics of fertilizing soil by organic matters and improving soil and the quick-acting function of inorganic nutrients; the nutrient content of nitrogen, organic matters, soluble phosphorus and soluble potassium in the soil can be increased, the population and the quantity of beneficial microorganisms in the soil can be increased, and meanwhile, the disease resistance, drought resistance and metabolic function of crops can be enhanced; the comprehensive popularization and application of the fertilizer can continuously improve the utilization rate of the fertilizer; has positive effects on improving crop yield, improving crop quality and increasing the economic income of farmers. In addition, organic components in the complete biological organic fertilizer can effectively replace partial chemical nutrients, so that the application amount of the fertilizer is reduced, and the crop yield is stabilized or promoted. Meanwhile, the additional values of organic fertilizers and bio-organic fertilizers can be greatly improved by research and development of novel full-element bio-organic fertilizers, so that the development of enterprises of the type is further promoted, and the organic wastes are deeply recycled to protect the environment.
Plant Growth-promoting Rhizobacteria (PGPR) refers to a microorganism that is present in the microenvironment of the soil around the surface and root system of the Plant rhizosphere and can significantly promote the Growth of plants by direct or indirect means. However, the current market generally adopts a single-strain bio-organic fertilizer, the single strain generally has the defects of functional diversity deficiency, poor resistance load and the like, and the product is difficult to completely meet the requirements of various nutrient elements of crops. The characteristics of the diversity of the strains of the complex flora enable the complex flora to adapt to various habitats and coordinate with each other. In agriculture, the compound flora can improve soil fertility and accelerate the decomposition and transformation of soil nutrients, thereby achieving the effects of saving fertilizer and promoting the growth of plant roots. Therefore, the development of organic-inorganic full-element bio-organic fertilizer products containing compound bacteria becomes a hotspot and a difficulty in the current fertilizer field.
Disclosure of Invention
The invention aims to solve the defects of functional diversity deficiency and poor resistance load of a single strain, low content of effective nutrients, slow fertilizer efficiency, low economic benefit, no nutrient element contained in a biological fertilizer and the like of the traditional organic fertilizer, develop a compound bacterium-containing organic-inorganic full-element biological organic fertilizer product and clarify the application effect of the fertilizer.
The technical purpose of the invention is realized by the following technical scheme.
The organic-inorganic complete-element bio-organic fertilizer containing the composite bacteria is formed by mixing 88-92% of the composite bacteria bio-organic fertilizer, 1-3% of urea, 4-5% of calcium superphosphate and 1.5-3.5% of potassium sulfate by dry weight percentage, the sum of the dry weight percentages of the composite bacteria bio-organic fertilizer, the urea, the calcium superphosphate and the potassium sulfate is 100%, the composite bacteria bio-organic fertilizer is the composite bacteria bio-organic fertilizer containing bacillus amyloliquefaciens SQR9, pseudomonas Y2 and seawater bacillus L-60, the collection number of the bacillus amyloliquefaciens SQR9 is CGMCC NO.5808, the collection number of the pseudomonas Y2 is CGMCC NO.10242, the collection numbers of the bacillus marinus SQR 7-60 are CGMCC NO. 1025636.
As the optimization of the organic-inorganic complete biological organic fertilizer product containing the composite bacteria, the formula comprises the following components in terms of the mass of the complete biological organic fertilizer: 905 kg/ton of composite bacteria-containing bio-organic fertilizer, 30 kg/ton of urea, 40 kg/ton of calcium superphosphate and 25 kg/ton of potassium sulfate.
The composite bacteria-containing organic-inorganic all-element bio-organic fertilizer is preferably prepared by mixing a bio-organic fertilizer containing bacillus amyloliquefaciens SQR9, a bio-organic fertilizer containing pseudomonas Y2 and a bio-organic fertilizer containing bacillus marinus L-60 according to the dry weight mass ratio of 1: 0.8-1.5.
As further optimization of the organic-inorganic complete-element biological organic fertilizer containing the composite bacteria, a biological organic fertilizer containing Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808, a biological organic fertilizer containing Pseudomonas Y2 with the preservation number of CGMCC NO.10242 and a biological organic fertilizer containing Bacillus marinus L-60 with the preservation number of CGMCC NO.10285 are mixed according to the dry weight mass ratio of 1:1: 1.
As the optimization of the organic-inorganic full-element biological organic fertilizer containing the composite bacteria, the total nitrogen, phosphorus and potassium nutrients are more than or equal to 8.0 percent, the mass fraction (calculated by dry basis) of organic matters is more than or equal to 30 percent, the pH value is 5.5-7.5, and the water content is less than or equal to 30 percent.
As the optimization of the organic-inorganic full-element bio-organic fertilizer containing composite bacteria, the total content of functional bacteria is more than or equal to 2 × 107CFU/g, single bacterium content is more than or equal to 1 × 106CFU/gram.
The preparation method of the organic-inorganic complete-element biological organic fertilizer containing the composite bacteria comprises the steps of adding 15-20% (V/W) acidolysis amino acid solution into decomposed chicken manure compost, carrying out after-ripening for 3-5 days, turning the compost once every day during the after-ripening period to increase the pH to 5.5, respectively adding single microbial fermentation liquid with the mass of 10% of the dry weight of the compost, carrying out solid fermentation for 5 days, evaporating the water content of the solid fermentation product to below 30% under the condition that the temperature is not higher than 60 ℃ to obtain the single-bacterial biological organic fertilizer, uniformly mixing the prepared biological organic fertilizer containing Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808, the biological organic fertilizer containing Y pseudomonas 2 with the preservation number of CGMCC NO.10242 and the biological organic fertilizer containing Bacillus marianus L-60 with the preservation number of CGMCC NO.10285 according to the dry weight ratio of 1: 0.8-1.5: 0.5-1.5% of the dry weight of the composite bacteria organic fertilizer, and mixing the prepared biological organic fertilizer containing 3-92% of the potassium sulfate and the total weight of the biological organic fertilizer and the urea to 100% of the composite bacteria to the compound organic fertilizer.
The formula of the composite bacteria-containing biological organic fertilizer is formed by uniformly mixing 3 single-bacteria biological organic fertilizers according to the dry weight ratio of 1:1:1, wherein the total content of functional bacteria is more than or equal to 2 × 107CFU/g, single bacterium content is more than or equal to 1 × 106CFU/gram. The single-bacterium-containing bio-organic fertilizer is prepared by aerobic composting of chicken manure as a raw material to prepare decomposed compost, adding 15-20% (V/W) acidolysis amino acid solution into the decomposed compost, after-ripening for 3-5 days, turning over the compost once a day during the after-ripening period to increase the pH to 5.5, adding microbial fermentation broth, performing solid fermentation for 5 days, and performing solid fermentation at a temperature of not more than 60 DEG CThe water content of the solid fermentation product is evaporated to be below 30 percent to obtain a single-bacterium bio-organic fertilizer product, the pH of the final product is more than or equal to 5.5 and less than or equal to 7.5, the fermentation liquid is prepared by the following method that an NA culture medium is sterilized, then any strain in a composite bacterium combination is inoculated, then liquid fermentation is carried out, the temperature range of fermentation production is 30-37 ℃, the stirring speed is 170-200 r/min, the fermentation time is 24h, and the bacterium content in the fermentation liquid is more than or equal to 1 × 10 after fermentation9CFU/ml. the NA culture solution is prepared by adding 10g of anhydrous glucose, 5g of peptone, 0.5g of yeast powder and 3g of beef extract into sterile deionized water, diluting to 1000ml, keeping the pH natural, and sterilizing at 121 ℃ for 20min, taking preparation of a 1L culture medium as an example.
The preparation method of the amino acid hydrolysate is carried out by referring to an authorized patent, and the patent name is zero-pollution harmless treatment and high-added-value resource utilization process for livestock and poultry dead of diseases, and the authorization number is Z L201410429510.0.
Preferably, in the present invention, 15% (V/W) of the acid hydrolyzed amino acid solution is added to the prepared decomposed compost.
On the basis of the composite bacteria biological organic fertilizer, 90.5 percent of multi-strain biological organic fertilizer, 3 percent of urea, 4 percent of calcium superphosphate and 2.5 percent of potassium sulfate are respectively and uniformly mixed according to the proportion (dry weight percentage), and the water content of the solid mixture is evaporated to be below 30 percent under the condition that the temperature is not more than 60 ℃, thus obtaining the composite bacteria-containing organic-inorganic full-element biological organic fertilizer.
The full-element bio-organic fertilizer contains functional bacteria more than or equal to 2 × 107CFU/g, total nitrogen 3%, total phosphorus (P)2O5) 2.41% and total potassium (K)2O)2.84 percent, total nitrogen, phosphorus and potassium nutrients of 8.25 percent (more than or equal to 8.0 percent), the mass fraction of organic matters (calculated by dry basis) is more than or equal to 30 percent, the pH value is 5.5-7.5, and the water content is less than or equal to 30 percent.
The organic-inorganic full-element bio-organic fertilizer containing the compound bacteria is applied to promoting the growth of tomatoes, eggplants, corns and/or Chinese cabbages.
Has the advantages that:
the invention provides a preparation method of a compound bacteria-containing complete bio-organic fertilizer product, wherein the compound bacteria comprise bacillus amyloliquefaciens SQR9, pseudomonas Y2 and bacillus marinus L-60, and have remarkable growth promotion superiority compared with any single bacteria, and also have remarkable growth promotion superiority compared with a compound bacteria complete bio-organic fertilizer (pseudomonad Y2 is replaced by growth promotion strain 84) replacing one bacteria.
Biological material preservation information
SQR9, classified name Bacillus amyloliquefaciens, deposited in China general microbiological culture Collection center, with the deposit address of China academy of sciences microorganism institute No. 3, West Lu No.1, North Chen West Lu, No.1, in the area of Tokyo, Beijing, the date of deposition is 2012-2-27 days, and the number of deposition is CGMCC No. 5808. the strain is disclosed in Z L201710797445.0.
Y2, classified and named as Pseudomonas frederiksbergensis, which is preserved in China general microbiological culture Collection center, wherein the preservation address is the microbiological research institute of China academy of sciences No. 3, West Lu No.1 Hopkins, North American district, Beijing, the preservation date is 12-25 months in 2014, and the preservation number is CGMCC No. 10242. the strain is disclosed in Z L201510399056.3.
L-60, classified and named as Bacillus marinus aquimaris, which is preserved in China general microbiological culture Collection center, wherein the preservation address is the microbiological research institute of China academy of sciences No. 3, West Lu No.1 Hopkin, Naja, Beijing, the preservation date is 2015, 01 month and 07 day, and the preservation number is CGMCC No. 10285. the strain is disclosed in 201510982083.3.
Detailed Description
Example 1 preparation of organic-inorganic full-element bio-organic fertilizer containing composite bacteria:
inoculating three kinds of growth-promoting bacteria to the prepared liquid NA culture medium under aseptic condition,
bacillus amyloliquefaciens SQR9, Pseudomonas Y2, Bacillus marinus L-60, and 84 strains (see in the description) stored in a glycerin pipe of a refrigerator at-80 deg.CLiu Xiaoyu, master thesis, title of screening rhizosphere growth promoting strain and its compound microbial fertilizer research and fertilizer efficiency research, Nanjing agriculture university), marking and activating on NA solid culture medium, placing in 37 deg.C incubator overnight, lightly scraping single colony of SQR9, Y2, L-60 or 84 on a flat plate with inoculating loop, then respectively placing on NA liquid culture medium, then placing on 30 deg.C shaking table at 170rpm for 12h, then inoculating on liquid NA culture medium according to 0.1% (v/v), shaking for 36h to obtain SQR9, Y2, L-60 and 84 fermentation liquor (effective bacterial count is up to 10)9CFU·mL-1) And then standby.
Adding 15% (V/W) acid hydrolysis amino acid solution into the prepared decomposed chicken manure compost, uniformly mixing, balancing for 3-5 days, raising the pH value of the mixture pile to be more than 5.5, respectively inoculating the fermented microbial fermentation liquid into the mixture pile, fully mixing each pile, and then performing solid fermentation for 5 days, uniformly mixing SQR9, Y2 and L-60 or SQR9, Y2 and 84 in the dry weight ratio of 1:1:1 in the four single-bacterium bio-organic fertilizers prepared after the fermentation is completed, detecting the water content of the solid mixed fertilizer, if the water content is higher than 30%, evaporating the water content of the solid mixture to be lower than 30% under the condition that the temperature is not higher than 60 ℃, thus obtaining two multi-strain (3 strain) bio-organic fertilizers.
On the basis of the multi-strain biological organic fertilizer, the multi-strain biological organic fertilizer is respectively and uniformly mixed according to the mixture ratio (dry weight ratio) of 90.5 percent of multi-strain biological organic fertilizer, 3 percent of urea, 4 percent of calcium superphosphate and 2.5 percent of potassium sulfate, and the all-element biological organic fertilizer contains functional bacteria which are more than or equal to 2 × 107CFU/g, total nitrogen 3%, total phosphorus (P)2O5) 2.41% and total potassium (K)2O)2.84 percent, the mass fraction (calculated by dry basis) of organic matters is more than or equal to 30 percent, the pH value is 5.5-7.5, and the water content is less than or equal to 30 percent.
Example 2 potted plant growth promotion test for different single bacteria and composite bacteria
The pot experiment is carried out in a greenhouse of national center for engineering and technology of organic fertilizers (Yixing) and the tested tomato is a Shanghai tomato 903 without pathogenic bacteria, 7 treatments are set in the experiment, namely blank treatment CK, SQR9 bacterial liquid treatment SQR9, Y2 bacterial liquid treatment Y2, L60 bacterial liquid treatment L60, 84 bacterial liquid treatment 84, composite strain SQR9+ Y2+84 bacterial liquid treatment SQR9+ Y2+84, composite strain SQR9+ Y2+ L60 treatment SQR9+ Y2+ L60, and each treatment is carried out in eight times.
The SQR9+ Y2+ L60 treatment is higher than the blank treatment, each single bacterium treatment and the composite SQR9+ Y2+84 treatment in plant height and stem thickness, and has no difference in stem thickness in dry weight fresh weight of the plant, the SQR9+ Y2+ L60 treatment has difference with other treatments in dry weight fresh weight, wherein the difference with the 84 treatment and the blank treatment is significant, and the growth promoting effect of the composite bacterium SQR9+ Y2+ L60 is shown to be significantly better than that of each single bacterium and the SQR9+ Y2+84 composite bacterium.
TABLE 1 Effect of different treatments on tomato plant height Stem thickness
TABLE 2 Effect of different treatments on the dry and fresh weight of tomato plants
Example 3 Effect of different fertilization treatments on eggplant yield
The field experiment is from 3 months in 2019 to 7 months in 2019, the experimental site is a vegetable and flower scientific research institute located in Nanjing OF Wuchu West, and the experiment is totally provided with 6 treatments, namely treatment 1(CK) for completely not applying fertilizers, treatment 2(OF) for applying a common cow dung organic fertilizer to a base fertilizer and 7.4kg OF the common cow dung organic fertilizer in each cell, treatment 3(CF) for applying a chemical fertilizer to the base fertilizer and keeping the total nutrients consistent with treatment 2, treatment 4(BF1) for applying an SQR9 biological organic fertilizer to the base fertilizer and keeping the rest nutrients balanced with the chemical fertilizer and keeping the total nutrients consistent with treatment 2, treatment 5(BF2) for applying an SQR9+ Y2+ L60 biological organic fertilizer to the base fertilizer and keeping the rest nutrients balanced with the chemical fertilizer and treatment 2, and treatment 6(BF3) for applying an SQR9+ Y2+84 biological organic fertilizer and keeping the rest nutrients balanced with the base fertilizer and the total nutrients balanced with treatment 2 and keeping the total nutrients 8m OF each cell in each cell2And (4) performing conventional management. And (4) harvesting the mature eggplant, weighing and measuring the yield.
The growth promoting effect OF different treatments on eggplants is shown in table 1, the yield OF biological organic fertilizer treatment (BF1, BF2 and BF3) is increased by 118.10%, 126.21% and 80.34% respectively relative to blank treatment (CK), is increased by 52.95%, 58.64% and 26.48% respectively relative to common organic fertilizer treatment (OF), and is increased by 24.49%, 29.12% and 2.94% respectively relative to fertilizer treatment (CF), wherein the yield OF the biological organic fertilizer containing the composite bacteria SQR9+ Y2+ L60 applied to 5(BF2) base fertilizer is obviously superior to the yield OF the biological organic fertilizer containing the composite bacteria SQR9 applied to the base fertilizer and the yield OF the biological organic fertilizer containing the composite bacteria SQR9+ Y2+84 applied to the base fertilizer, and the growth promoting effect OF the composite bacteria R9+ Y2+ L60 is obviously superior to the yield OF single bacteria SQR9 and the composite bacteria SQR9+ Y2+ Y39.
TABLE 1 growth promotion of eggplant test yield
Example 4 test of growth promoting effect of corn field composite bacteria complete element bio-organic fertilizer replacing 15% nitrogen
The field test of the corn is carried out in the basic agricultural field of Hainan county in Nantong city from 4 months to 8 months in 2019, and the test is provided with four treatments of 1) CK without applying any fertilizer, 2) CF treatment and fertilizer application, and 3) BOFT treatment, wherein nitrogen in a biological organic fertilizer containing composite bacteria SQR9+ Y2+ L-60 full-element biological organic fertilizer is used for replacing 15% of nitrogen in the fertilizer treatment, the rest nutrients are applied with the fertilizer, the fertilizer application is carried out with the treatment of 2 and other nutrients, each treatment is repeated for 3 times, the length of each cell is 7.5m, the width of each cell is 4.75m, the area is 35.625m2, the fertilizer application mode is that 0.92kg of urea is applied to each cell except the CK treatment, the conventional management is carried out, and the yield is measured by the weight of harvested fruits after the.
The corn growth promoting effect is shown in the table 2, compared with the CK, the BOFT treatment yield is increased by 40.18%; CF increased by 10.43% compared to treated BOFT.
TABLE 2 corn growth promotion test yield
Example 5 growth promoting effect test of composite bacteria full-element bio-organic fertilizer in corn field
The field test OF corn is carried out in the basic farmland OF Haian county in Nantong city from 4 months to 8 months in 2019, and the test is set to four treatments, namely 1) CK treatment without applying any fertilizer, 2) OF treatment with applying common organic fertilizer and applying 10.68kg per cell, 3) CF treatment, chemical fertilizer application, nutrient fertilization such as 2 treatment, 4) BOF treatment, SQR9+ Y2+ L-60 full-element biological organic fertilizer containing compound bacteria and nutrient fertilization such as 2 treatment, 3 times OF treatment are repeated, each cell is 7.5m in length, 4.75m in width and 35.625m in area2The top dressing mode is that except CK treatment, 0.92kg of urea is applied to each cell and is managed conventionally. And (4) harvesting the mature corn, weighing and measuring the yield.
The corn growth promoting effect is shown in Table 3, and compared with the CK, the BOF treated by the method increases the yield by 65.07%; compared with CF, the BOF treated by the method is increased by 30.04%; compared with OF, the BOF treated by the method has 14.76 percent OF yield increase.
TABLE 3 corn growth promotion test yield
Example 6 experiment of growth promoting effect of Chinese cabbage composite bacteria complete bio-organic fertilizer replacing 15% nitrogen
The field test of Chinese cabbage is carried out in the basic agricultural field of Haian county in Nantong city from 8 months to 12 months in 2019, and the test is set to four treatments, namely 1) CK treatment without applying any fertilizer, 2) CF treatment and fertilizer application, 3) BOFT treatment, nitrogen in a biological organic fertilizer containing compound bacteria SQR9+ Y2+ L-60 full-element biological organic fertilizer is used for replacing 15% of nitrogen in the fertilizer treatment, the rest nutrients are applied with the fertilizer, and the fertilizer application and the treatment 2 and other nutrients are carried out, wherein each treatment is carried out for 3 times, each treatment is 7.5m in length, 4.75m in width and 35.625m in area2The top dressing mode is that except CK treatment, 0.92kg of urea is applied to each cell and is managed conventionally. And (4) harvesting the mature Chinese cabbage, weighing and measuring yield.
The growth promoting effect of the Chinese cabbage is shown in Table 4, compared with CK, the yield of treated BOFT is 204.67%; compared with CF, the BOFT treatment yield is increased by 22.52%.
TABLE 4 growth promotion of Chinese cabbage test yield
Example 7 growth promoting effect test of composite bacteria complete-element bio-organic fertilizer for Chinese cabbage field
The field test OF Chinese cabbage is carried out in the basic agricultural field OF Haian county in Nantong city from 8 months to 12 months in 2019, and the test is set to four treatments, namely 1) CK treatment without applying any fertilizer, 2) OF treatment with applying common organic fertilizer and applying 10.68kg per cell, 3) CF treatment, chemical fertilizer application, nutrient fertilization such as 2 treatment, 4) BOF treatment, SQR9+ Y2+ L-60 full-element bio-organic fertilizer containing compound bacteria and nutrient fertilization such as 2 treatment, 3 times OF treatment are repeated, each cell is 7.5m long, 4.75m wide and 35.625m area2The top dressing mode is that except CK treatment, 0.92kg of urea is applied to each cell and is managed conventionally. And (4) harvesting the mature Chinese cabbage, weighing and measuring yield.
The growth promoting effect of the Chinese cabbage is shown in Table 5, compared with BOF and CK, the BOF yield increase of the Chinese cabbage is 192.67% respectively; compared with OF, the BOF treatment yield is increased by 23.66%; compared with CF, the treated BOF is increased by 17.69%.
TABLE 5 growth promotion of Chinese cabbage test yield
The results show that the application of the full-element bio-organic fertilizer has obvious yield increasing effect on the corns and the Chinese cabbages, and the multi-strain full-element bio-organic fertilizer has good growth promoting effect on the corns and the Chinese cabbages.
Claims (10)
1. The organic-inorganic complete-element bio-organic fertilizer containing the composite bacteria is characterized by being formed by mixing 88-92% of composite bacteria bio-organic fertilizer, 1-3% of urea, 4-5% of calcium superphosphate and 1.5-3.5% of potassium sulfate by dry weight percentage, wherein the sum of the dry weight percentages of the composite bacteria bio-organic fertilizer, the urea, the calcium superphosphate and the potassium sulfate is 100%, the composite bacteria bio-organic fertilizer is the composite bacteria bio-organic fertilizer containing Bacillus amyloliquefaciens (SQR 9), Pseudomonas freudenreichiksgensi (Pseudomonas freudenreksgesii) Y2 and seawater Bacillus (Bacillus aquimaris) L-60, the collection number of the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR9 is CGMCC NO.5808, the collection number of the Pseudomonas amyloliquefaciens (Pseudomonas solanacesi) Y2 is CGMCC NO. 38760, and the collection number of the Pseudomonas amyloliquefaciens (Bacillus amyloliquefaciens) Y38732 is 38760-64-60.
2. The compound bacteria-containing organic-inorganic full-element bio-organic fertilizer as claimed in claim 1, wherein the composition of the compound bacteria-containing bio-organic fertilizer is 905 kg/ton, 30 kg/ton urea, 40 kg/ton calcium superphosphate and 25 kg/ton potassium sulfate.
3. The all-element bio-organic fertilizer as claimed in claim 1, characterized in that the composite bacteria bio-organic fertilizer is prepared by mixing a bio-organic fertilizer containing Bacillus amyloliquefaciens SQR9, a bio-organic fertilizer containing Pseudomonas Y2 and a bio-organic fertilizer containing Bacillus marinus L-60 according to a dry weight ratio of 1: 0.8-1.5.
4. The complete-element bio-organic fertilizer as claimed in claim 3, characterized in that the compound bacteria bio-organic fertilizer is prepared by mixing a bio-organic fertilizer containing Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808, a bio-organic fertilizer containing Pseudomonas Y2 with the preservation number of CGMCC NO.10242 and a bio-organic fertilizer containing Bacillus marinus L-60 with the preservation number of CGMCC NO.10285 according to the dry weight ratio of 1:1: 1.
5. The all-element bio-organic fertilizer according to claim 1, characterized in that the total nitrogen, phosphorus and potassium nutrients of the compound bacteria-containing organic-inorganic all-element bio-organic fertilizer are more than or equal to 8.0%, the mass fraction of organic matters is more than or equal to 30% on a dry basis, the pH value is 5.5-7.5, and the water content is less than or equal to 30%.
6. The full-element bio-organic fertilizer as claimed in claim 1, wherein the total content of functional bacteria in the organic-inorganic full-element bio-organic fertilizer containing composite bacteria is not less than 2 × 107CFU/g, single bacterium content is more than or equal to 1 × 106CFU/gram.
7. The preparation method of the compound bacteria-containing organic-inorganic complete organic fertilizer is characterized by comprising the steps of adding 15-20% (V/W) acidolysis amino acid solution into decomposed chicken manure compost, conducting after-ripening for 3-5 days, turning the compost once every day during the after-ripening period to increase the pH to 5.5, respectively adding single microbial fermentation liquid with the mass of 10% of the dry weight of the compost, conducting solid fermentation for 5 days, evaporating the water content of the solid fermentation product to below 30% under the condition that the temperature does not exceed 60 ℃ to obtain the single microbial bio-organic fertilizer, uniformly mixing the prepared bio-organic fertilizer containing Bacillus amyloliquefaciens SQR9 with the preservation number of CGMCC NO.5808, the bio-organic fertilizer containing Pseudomonas Y2 with the preservation number of CCNO.10242 and the bio-organic fertilizer containing seawater bacillus L-60 with the preservation number of CGMCC NO.10285 according to the mass ratio of 1: 0.8-1.5% to 1.5% of the dry weight of the compound bacteria-organic fertilizer, and mixing the prepared bio-organic fertilizer containing potassium sulfate with the total weight of the compound bacteria-urea according to the total weight of the compound bacteria-organic fertilizer of 1.88% of the compound calcium phosphate, wherein the total organic fertilizer is 100-urea and the total weight of the compound fertilizer is 100-urea.
8. The preparation method of claim 7, wherein the single microorganism fermentation broth is prepared by sterilizing NA medium, inoculating with Bacillus amyloliquefaciens SQR9, Pseudomonas Y2, Bacillus marinus L-60, fermenting at 30-37 deg.C under stirring at 170-200 rpm for 24 hr to obtain fermentation broth with bacteria content of 1-1 × 109CFU/ml。
9. The preparation method of claim 7, wherein the compound bacteria-containing organic-inorganic full-element bio-organic fertilizer is prepared by uniformly mixing 90.5% of compound bacteria bio-organic fertilizer, 3% of urea, 4% of calcium superphosphate and 2.5% of potassium sulfate in terms of dry weight ratio on the basis of the compound bacteria bio-organic fertilizer, and evaporating the water content of the solid mixture to below 30% at a temperature of not more than 60 ℃.
10. The application of the composite bacteria-containing organic-inorganic full-element bio-organic fertilizer as defined in any one of claims 1-6 in promoting the growth of tomatoes, eggplants, corns and/or cabbages.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112794764A (en) * | 2021-02-03 | 2021-05-14 | 四川农业大学 | Complete-element biological organic fertilizer and preparation method thereof |
| CN112946239A (en) * | 2021-02-01 | 2021-06-11 | 中国农业科学院农业资源与农业区划研究所 | Method for measuring and calculating maximum manure amount allowed to be applied to soil based on soil phosphorus environment threshold |
| CN113372171A (en) * | 2021-06-21 | 2021-09-10 | 华强化工集团股份有限公司 | Full-element bio-organic fertilizer for corn and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104788143A (en) * | 2015-03-31 | 2015-07-22 | 南京农业大学 | Method for producing bio-organic fertilizer from acidolysis solution and decomposed manure and product |
| CN107686406A (en) * | 2017-09-20 | 2018-02-13 | 南京农业大学 | A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely |
| CN108610177A (en) * | 2018-06-22 | 2018-10-02 | 南京农业大学 | A kind of special trichoderma of banana crops first bio-organic fertilizer and its preparation method and application entirely |
| CN108727135A (en) * | 2018-06-22 | 2018-11-02 | 南京农业大学 | A kind of special trichoderma of perennial fruit tree first biological organic fertilizer and its application entirely |
-
2020
- 2020-03-31 CN CN202010242923.3A patent/CN111393206A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104788143A (en) * | 2015-03-31 | 2015-07-22 | 南京农业大学 | Method for producing bio-organic fertilizer from acidolysis solution and decomposed manure and product |
| CN107686406A (en) * | 2017-09-20 | 2018-02-13 | 南京农业大学 | A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely |
| CN108610177A (en) * | 2018-06-22 | 2018-10-02 | 南京农业大学 | A kind of special trichoderma of banana crops first bio-organic fertilizer and its preparation method and application entirely |
| CN108727135A (en) * | 2018-06-22 | 2018-11-02 | 南京农业大学 | A kind of special trichoderma of perennial fruit tree first biological organic fertilizer and its application entirely |
Non-Patent Citations (1)
| Title |
|---|
| 李静等: "含复合菌群生物育苗基质的研制及其育苗效果", 《南京农业大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112946239A (en) * | 2021-02-01 | 2021-06-11 | 中国农业科学院农业资源与农业区划研究所 | Method for measuring and calculating maximum manure amount allowed to be applied to soil based on soil phosphorus environment threshold |
| CN112794764A (en) * | 2021-02-03 | 2021-05-14 | 四川农业大学 | Complete-element biological organic fertilizer and preparation method thereof |
| CN113372171A (en) * | 2021-06-21 | 2021-09-10 | 华强化工集团股份有限公司 | Full-element bio-organic fertilizer for corn and preparation method thereof |
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