CN103409351A - Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same - Google Patents

Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same Download PDF

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CN103409351A
CN103409351A CN2013103605158A CN201310360515A CN103409351A CN 103409351 A CN103409351 A CN 103409351A CN 2013103605158 A CN2013103605158 A CN 2013103605158A CN 201310360515 A CN201310360515 A CN 201310360515A CN 103409351 A CN103409351 A CN 103409351A
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growth
organic fertilizer
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banana
njauyl
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沈其荣
黄启为
俞鲁
张楠
何欣
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Nanjing Agricultural University
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Nanjing Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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Abstract

The invention relates to a growth promoting strain used for promoting banana growth and a microbial organic fertilizer produced with the same, belonging to the intensive agriculture production technology. A growth promoting strain NJAUYL-25 is isolated and is inoculated into a microbial decomposition mixture of thoroughly decomposed pig manure composts and rapeseed meal to undergo solid fermentation to prepare the microbial organic fertilizer, namely the microbial organic fertilizer with the function of promoting banana growth. The fertilizer contains more than 1*108cfu/g of growth promoting bacteria and has total nitrogen content of 4-5% (more than 90% of organic nitrogen), total nitrogen, phosphorus and potassium nutrient of 6-10% and organic content not less than 45%. The test results indicate that after the microbial organic fertilizer, compared with chemical fertilizers which are only applied, is applied to the continuous cropping soil of bananas, the fresh weights and heights of banana seedlings are respectively increased by 1.89-5.77 times and 45.6-122.0% 30-50 days after the banana seedlings are planted. Banana growth can be effectively promoted by applying the bio-organic fertilizer in successive years.

Description

For promoting growth-promoting bacterial strain and the microorganism organic fertilizer thereof of Banana Growth
One, technical field
The present invention relates to, be used to promoting growth-promoting bacterial strain and the microorganism organic fertilizer thereof of Banana Growth, belong to the intensive agriculture production technical field.
Two, background technology
Banana is one of important fruit in the world, and tool, in high economic worth, occupies critical role in the development of the national economy of China.In recent years, in the banana planting process, long-term a large amount of chemical fertilizer drops into the edatope of having destroyed Banana Growth, and Content of available nutrient in soils is descended, the biological activity of soil degradation, and soil beneficial microorganism kind, quantity reduce, and cause the banana underproduction year after year.Therefore, exploitation promotes the new-type fertilizer of Banana Growth, significant for the production of cash crop.
On the other hand, China's crop material (paddy rice, wheat, corn etc.) on-site incineration, the feces of livestock and poultry after large-scale cultivation have been thrown aside etc. not only severe contamination everywhere environment, also greatly having wasted can be as the raw material of fertilizer and biological organic fertilizer product.A large amount of nutrients resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened significantly the ability of China's agricultural sustainable development.These solid organic castoffs being made to Commodity Organic Fertilizer and be manured into soil, is that the nutritive element from taking away because of crop harvesting soil is returned to the effective way in soil to greatest extent.These solid organic castoff thermophilic fermentations are prepared into to matured compost, then will have good application prospect used as the carrier of biological (the containing specific beneficial microorganism) organic fertilizer of preparation.
Biological organic fertilizer refers to the microorganism (growth-promoting, biological and ecological methods to prevent plant disease, pests, and erosion etc.) that will have specific function and mainly take plant and animal residues (as agricultural crop straw, feces of livestock and poultry etc.) for originating and through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited, having the fertilizer of microbial fertilizer and fertilizer effect concurrently.Its essential characteristic is to contain beneficial microorganism specific function, that show certain fertilizer effect, and these specific beneficial microorganism vital movements are key factors that biological organic fertilizer is better than other fertilizer.Large quantity research shows: in agriculture production biological organic fertilizer strengthening crop anti-adversity, crop nutrition content is provided, increase fertilizer efficiency, improve crop to the absorption of nutrient, regulate the aspect such as soil acidity or alkalinity and bringing into play positive effect.
This research is intended from screening growth-promoting bacterial strain in the banana plant root system of healthy growth the field of banana long term growth and rhizosphere soil; with matured compost and other suitable carriers, adopt again the microorganism organic fertilizer of suitable explained hereafter promotion Banana Growth, for the banana large-scale production provides technical products.
Three, summary of the invention
1. technical problem
The object of the present invention is to provide be used to promoting growth-promoting bacterial strain and the microorganism organic fertilizer thereof of Banana Growth, the growth-promoting function is obvious, can guarantee shaping up of intensive agricultural.
2. technical scheme
For promoting the growth-promoting bacterial strain NJAUYL-25 of Banana Growth, for bacillus amyloliquefaciens belongs to (Bacillus amyloliquefaciens).On May 17th, 2013 was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and the culture presevation of NJAUYL-25 number is: CGMCC NO.7626.
The Main Biological of NJAUYL-25 bacterial strain is: thalline Gram-positive G+, cell are shaft-like, produce gemma, strictly aerobic, the Catalase determination positive, V.P. reacting positive, methyl red test feminine gender, glucose fermentation, sucrose, dextrin, N.F,USP MANNITOL, wood sugar, pectinose produce sour aerogenesis, fermentation fructose, maltose, rhamnosyl, inositol aerogenesis do not produce acid, nonfermented lactose, nitrate reductase enzyme positive, the Starch Hydrolysis positive, gelatin liquefaction positive.
Microorganism organic fertilizer with the growth-promoting bacterium of above-mentioned promotion Banana Growth produces, contain 1 * 10 in this microorganism organic fertilizer 8Above NJAUYL-25, the total nitrogen content mass ratio of cfu/g is 4-5%, and in full nitrogen, mass ratio is organonitrogen more than 90%, and total nitrogen phosphorus potassium nutrition mass ratio is 6-10%, organic content mass ratio>=45%.
The production method of described microorganism organic fertilizer is characterized by:
(1) growth-promoting bacterial strain NJAUYL-25 is carried out to liquid fermenting production, the condition of its fermentative production is: nutrient solution is the NA substratum, pH6.0-7.0, leavening temperature scope 30-35 ℃, stirring velocity is 180-300 rev/min, and fermentation time is 48hr., the bacteria containing amount of fermented liquid or gemma amount>=1 * 10 10Cfu/ml; NA nutrient solution compound method used is (the preparation 1L substratum of take is example): glucose 10g, and peptone 5g, extractum carnis 3g, yeast powder 0.5g, be settled to 1000ml, and the pH value is adjusted to 7.2-7.4,115 ℃ of sterilizing 30min.
The microorganism enzymolysis mixture of the pig manure that (2) will become thoroughly decomposed and the dish dregs of rice ratio of 6: 4 in mass ratio mixes.Again the fermented liquid of above-mentioned NJAUYL-25 is inoculated in this mixture and carries out solid fermentation by the amount of 50L/ ton, in fermenting process, turning every day is 1 time, and temperature is no more than 50 ℃, ferments 7 days, and after fermentation ends, the quantity of growth-promoting bacterial strain NJAUYL-25 all reaches 1 * 10 8More than cfu/g.Obtain the pig manure of growth-promoting bacterial strain NJAUYL-25 and the solids mixing microbial inoculum of dish dregs of rice microorganism enzymolysis mixture.Finally under temperature is no more than the condition of 60 ℃, the water content of microbial organic fertilizer is evaporated to below 30%, packing is dispatched from the factory and is the microbial organic fertilizer that can promote the banana plant growth; Wherein
The pig manure germination index become thoroughly decomposed used is more than 98%, quality of organic matter ratio content >=45%, and the organonitrogen quality is 1.2-2.0% than content, water content mass ratio 25-30%;
The microorganism enzymolysis preparation method of mixture of the dish dregs of rice is, germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid that is CGMCC NO.1543 by preserving number joins in dish dregs of rice raw material, regulate water content 55-65% and pH6.0-7.5, carry out open solid fermentation, when rising to 50 ℃, leavening temperature starts immediately turning, turning every day later, make to pile temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation starts, the pH of stacking yard can constantly rise, during each turning, regulate water content and pH with containing acid substance (liquid), make them maintain respectively 55-65% and 6.0-7.0, when stopping, fermentation with containing the acid solution body, sprays again, until stacking yard pH is adjusted into 5.0, carry out again cryodrying or natural airing, be the microorganism enzymolysis mixture of the dish dregs of rice, its germination index of Chicken Manure Compost after used becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content >=45%, and the organonitrogen quality is 2-3% than content, water content is 25-30%.
3. beneficial effect
The present invention compares and has following advantage with organic fertilizer product in the market:
1) in this fertilizer product, contain the specificity high efficient strain NJAUYL-25 that promotes Banana Growth, its growth-promoting effect highly significant.Test-results shows: use this microorganism organic fertilizer in the non-continuous cropping soil of banana, can increase or significantly increase the fresh weight of different varieties banana seedlings, with single, compare to fertilize, the banana seedlings fresh weight increases 86.1-125.2% (No. 1, agriculture section and coke breeze, Brazilian fragrant tooth any of several broadleaf plants slightly descend but without significant difference); With single organic fertilizer, process relatively, the banana seedlings fresh weight increases 44.5-134.5%.In the banana continuous cropping soil, use this microorganism organic fertilizer, the banana seedlings plantation, after 30-50 days, can significantly increase fresh weight and the plant height of banana seedlings, compares to fertilize with single, and banana seedlings fresh weight and plant height increase respectively 1.89-5.77 doubly and 45.6-122.0%; With single organic fertilizer, compare, banana seedlings fresh weight and plant height increase respectively 0.78-1.99 doubly and 33.1-59.5%.Use this biological organic fertilizer year after year and can effectively promote Banana Growth.
2) this fertilizer is fertilizer, contains abundant organic matter (30-35%), and organonitrogen content is 4%, and major part is amino acid and micromolecule polypeptide.This product total phosphorous is 4.0%, and biological effectiveness is high especially, uses after this product and can help crop to spend smoothly phosphorus element critical porion in seedling stage.
3) this fertilizer is biological bacterial strain preparation, the series of problems not do not brought because of the use of chemical pesticide, thereby be conducive to the nuisanceless production of vegetables, the peasant can or reduce the consumption of other chemical fertilizer, this not only can be the peasant and reduces expenses, and is conducive to the outlet of agricultural-food.Simultaneously, this microbial fertilizer increases production function in addition, can be the peasant and increases income.
4) this microbial organic fertilizer can be used at nutrition pot, also can use in land for growing field crops, not only has the function of fertilizer, and wherein contained growth-promoting bacterium is worked by multiple growth-promoting mechanism, thereby reaches the effect that promotes Banana Growth.
Four, accompanying drawing explanation
The morphological specificity of Fig. 1 growth-promoting bacterial strain NJAUYL-25
Biological preservation
Bacterial strain NJAUYL-25, for bacillus amyloliquefaciens belongs to (Bacillus amyloliquefaciens), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 17th, 2013, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, culture presevation number is: CGMCC NO.7626.
Five, embodiment
(1) separation of growth-promoting antagonistic bacterium strain
Growth-promoting bacterial strain NJAUYL-25 separates in the morbidity garden of the Le Dongxian of Hainan Province banana plantation.
(2) evaluation of growth-promoting bacterial strain
Growth-promoting bacterial strain NJAUYL-25 is accredited as bacillus amyloliquefaciens and belongs to (Bacillus amyloliquefaciens).
The Main Biological of NJAUYL-25 bacterial strain is: thalline Gram-positive G+, cell are shaft-like, produce gemma, strictly aerobic, the Catalase determination positive, V.P. reacting positive, methyl red test feminine gender, glucose fermentation, sucrose, dextrin, N.F,USP MANNITOL, wood sugar, pectinose produce sour aerogenesis, fermentation fructose, maltose, rhamnosyl, inositol aerogenesis do not produce acid, nonfermented lactose, nitrate reductase enzyme positive, the Starch Hydrolysis positive, gelatin liquefaction positive.
(3) microbial inoculum production
1, growth-promoting bacterial strain NJAUYL-25 is inoculated in the NA nutrient solution and carries out liquid fermenting production, the condition of its fermentative production is: pH6.0-7.0, and leavening temperature scope 30-35 ℃, stirring velocity is 180-300 rev/min, fermentation time is 48 hours., fermented liquid contain bacterium or gemma amount>=1 * 10 10Cfu/ml.
The microorganism enzymolysis mixture of the pig manure that 2, will become thoroughly decomposed and the dish dregs of rice ratio of 6: 4 in mass ratio mixes.Again the fermented liquid of above-mentioned NJAUYL-25 is inoculated in this mixture and carries out solid fermentation by the amount of 50L/ ton, in fermenting process, turning every day is 1 time, and temperature is no more than 50 ℃, ferments 7 days, and after fermentation ends, the quantity of growth-promoting bacterium NJAUYL-25 reaches 1 * 10 8More than cfu/g.Finally under temperature is no more than the condition of 60 ℃, the water content of microbial organic fertilizer is evaporated to below 30%, packing is dispatched from the factory and is the microbial organic fertilizer that can promote the banana plant growth.The pig manure germination index become thoroughly decomposed used is more than 98%, and organic content >=45%, organonitrogen content are 1.2-2.0%, water content 25-30%.
In this microorganism organic fertilizer (promoting the special microorganism fertilizer of Banana Growth), contain 1 * 10 8Above NJAUYL-25, the total nitrogen content mass ratio of individual/g is 4-5%, and in full nitrogen, mass ratio is organonitrogen more than 90%, and total nitrogen phosphorus potassium nutrition mass ratio is that 6-10%, organic content mass ratio are not less than 45%.
The preparation method of the microorganism enzymolysis mixture of its Chinese food dregs of rice is (public, see patent of invention ZL200610086126.0, a kind of organism preparation method of agricultural amino acid and fertilizer product thereof): germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid that is CGMCC NO.1543 by preserving number joins in dish dregs of rice raw material, regulate water content 55-65% and pH6.0-7.5, carry out open solid fermentation, when rising to 50 ℃, leavening temperature starts immediately turning, turning every day later, make to pile temperature and maintain all the time 35-50 ℃ (totally 5 days), after solid fermentation starts, the pH of stacking yard can constantly rise, during each turning, regulate water content and pH with containing acid substance (liquid), make them maintain respectively 55-65% and 6.0-7.0, when stopping, fermentation with containing the acid solution body, sprays again, until stacking yard pH is adjusted into 5.0 left and right, carry out again cryodrying or natural airing, be the microorganism enzymolysis mixture (containing amino acid whose mixing material) of the dish dregs of rice-dish dregs of rice compost.
(4) pot experiment
1, test design
Adopt feeding block seedlings raising test and pot experiment research to contain the impact of the microorganism organic fertilizer of growth-promoting bacterial strain NJAUYL-25 on the banana seedlings growth.
3 processing: CK are all established in test: chemical fertilizer (contrast, not application of organic fertilizers or microbial organic fertilizer); OF: the mixture mixed by a certain percentage for the fertilizer-pig manure in BIO3 and dish dregs of rice compost; BIO3: the microbial organic fertilizer that contains growth-promoting bacterial strain NJAUYL-25.
Nutrition pot test soil be red soil (the non-continuous cropping soil of banana), and soil loading amount is every alms bowl 300 grams, and fertilizer and microbial organic fertilizer rate of fertilizer application are by heavy 2% the using of soil, and the application quantity of nitrogen phosphorus potassium of chemical fertilizer processing is pressed the amount of application calculating gained of fertilizer." Brazilian fragrant tooth any of several broadleaf plants " every processing 80 alms bowls (wherein 65 alms bowls are to prepare to use seedling for pot experiment), every processing 25 alms bowls of " No. 1, agriculture section " and " dwarf banana ".Tissue cultured seedling gathered the banana seedlings of nutrition pot on the 30th day after transplanting, measure its total fresh weight.
Pot experiment soil is that (soil passes the serious banana continuous cropping soil of blight morbidity to red soil, and wherein the spore suspension concentration of Pathogen Causing Banana Fusarium Wilt Fusarium oxysporum reaches 10 5CFU/g), every basin fills native 10kg, and every processing is established 3 districts Zu,Ge district group and repeated 10 times.Fertilizer and microorganism organic fertilizer rate of fertilizer application are by heavy 0.5% the using of soil, and the application quantity of nitrogen phosphorus potassium that chemical fertilizer is processed is by the amount calculating gained of the nitrogen phosphorus potassium of bringing in the fertilizer of using.Feeding block seedlings raising the 30th day, choose " Brazilian fragrant tooth any of several broadleaf plants " seedling that growing way is consistent and together with nutrition pot, be transplanted in the basin alms bowl of pot experiment, 1 of every basin field planting, conventional water and fertilizer management.At the 30th day, the 40th day of pot experiment and the 50th day, gather respectively total fresh weight and the plant height that banana seedlings is measured plant.
2, test-results
(1) feeding block seedlings raising test
Feeding block seedlings raising test-results (table 1) shows: during feeding block seedlings raising, with use chemical fertilizer relatively, use the growth (fresh weight increases respectively 86.1% and 125.2%) that the microbial organic fertilizer that contains growth-promoting bacterial strain NJAUYL-25 can significantly promote " No. 1, agriculture section " and fly " dwarf banana " Banana Seedlings, upgrowth situation and the chemical fertilizer processing of seedling remain basically stable for " Brazilian fragrant tooth any of several broadleaf plants ".With single organic fertilizer material, compare, the growth-promoting effect of microbial organic fertilizer is more remarkable, and " Brazilian fragrant tooth any of several broadleaf plants ", " anti-No. 1 of agriculture " and the fresh weight of " dwarf banana " seedling increase respectively 44.5%, 134.5% and 96.3%.
Table l uses the impact on different varieties banana seedlings fresh weight of the microbial organic fertilizer that contains growth-promoting bacterial strain NJAUYL-25
(nutrition bowl seedling transplanting the 30th day)
Figure BSA0000094000770000061
Annotate: CK: list to fertilize; OF: the mixture mixed by a certain percentage for the fertilizer-pig manure in BIO3 and dish dregs of rice compost;
BIO3: for containing the biological organic fertilizer of NJAUYL-25.Above data all mean mean value ± standard deviation (n=3), and the letter representation significance of difference after numeral reaches p<0.05 (Duncan), lower same.
(2) pot experiment
Results from pot experiment test (table 2) shows: with single, with single organic fertilizer, compare to fertilize, use the microbial organic fertilizer that contains growth-promoting bacterial strain NJAUYL-25 and can effectively alleviate the plant morbidity and significantly promote its growth.Measure respectively at the 30th, 40 and 50 days of pot experiment that " " fresh weight of plant and plant height, compare with single Brazilian fragrant tooth any of several broadleaf plants to fertilize, and using the plant fresh weight that biological organic fertilizer processes increases respectively 1.89-5.77 times and 45.6-122.0% with plant height; With single organic fertilizer, compare, that uses the biological organic fertilizer processing increases respectively 0.78-1.99 doubly and 33.1-59.5% for examination plant fresh weight and plant height.
Table 2 is used the microbial organic fertilizer that contains growth-promoting bacterial strain NJAUYL-25 and blight is connected to the impact of doing Brazilian fragrant tooth any of several broadleaf plants growth of seedling in soil
Figure BSA0000094000770000062
3, patent of the present invention is from promoting Banana Seedlings this key issue of growing to set about (table 1, table 2), develop a kind of microorganism organic fertilizer product that can significantly promote Banana Growth, test-results shows: use this microorganism organic fertilizer in the non-continuous cropping soil of banana, can increase or significantly increase the fresh weight of different varieties banana seedlings, with single, compare to fertilize, the banana seedlings fresh weight increases 86.1-125.2% (No. 1, agriculture section and coke breeze, Brazilian fragrant tooth any of several broadleaf plants slightly descend but without significant difference); With single organic fertilizer, process relatively, the banana seedlings fresh weight increases 44.5-134.5%.In the banana continuous cropping soil, use this microorganism organic fertilizer, the banana seedlings plantation, after 30-50 days, can significantly increase fresh weight and the plant height of banana seedlings, compares to fertilize with single, and banana seedlings fresh weight and plant height increase respectively 1.89-5.77 doubly and 45.6-122.0%; With single organic fertilizer, compare, banana seedlings fresh weight and plant height increase respectively 0.78-1.99 doubly and 33.1-59.5%.The mechanism of action of this fertilizer product be in this fertilizer product effective carbon source and effectively nitrogenous source to the fauna of the function stem in product, cultivate good condition be provided, make it to set up rapidly ecology and the food chain thereof of external source beneficial functions bacterial strain; On the other hand, in this fertilizer product, contain organonitrogen and the organophosphorus of high level, these nutritive substances are very favourable to the growth of banana, greatly improved vertical seedling rate and the anti-adversity ability of banana crops.

Claims (4)

1. be used to promoting the growth-promoting bacterial strain of Banana Growth, this bacterial strain NJAUYL-25, for bacillus amyloliquefaciens belongs to (Bacillus amyloliquefaciens), on May 17th, 2013 was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and culture presevation number is: CGMCC NO.7626.
The Main Biological of NJAUYL-25 bacterial strain is: thalline Gram-positive G+, cell are shaft-like, produce gemma, strictly aerobic, the Catalase determination positive, V.P. reacting positive, methyl red test feminine gender, glucose fermentation, sucrose, dextrin, N.F,USP MANNITOL, wood sugar, pectinose produce sour aerogenesis, fermentation fructose, maltose, rhamnosyl, inositol aerogenesis do not produce acid, nonfermented lactose, nitrate reductase enzyme positive, the Starch Hydrolysis positive, gelatin liquefaction positive.
2. the microorganism organic fertilizer of producing with the described growth-promoting bacterial strain of claim 1, contain respectively 1.0 * 10 in this microorganism organic fertilizer 8The NJAUYL-25 that cfu/g is above, the total nitrogen content mass ratio is 4-5%, and in full nitrogen, mass ratio is organonitrogen more than 90%, and total nitrogen phosphorus potassium nutrition mass ratio is 6-10%, organic content mass ratio>=45%.
3. the production method of the described microorganism organic fertilizer of claim 2 is characterized by:
(1) growth-promoting bacterial strain NJAUYL-25 claimed in claim 1 is inoculated into to the NA nutrient solution and carries out liquid fermenting production, the condition of its fermentative production is: pH6.0-7.0, leavening temperature scope 30-35 ℃, stirring velocity is 180-300 rev/min, fermentation time is 48 hours, fermented liquid contain bacterium or gemma amount>=1 * 10 10Cfu/ml; NA nutrient solution compound method used is, glucose 10g, and peptone 5g, extractum carnis 3g, yeast powder 0.5g, be settled to 1000ml, and the pH value is adjusted to 7.2-7.4,115 ℃ of sterilizing 30min;
The microorganism enzymolysis mixture of the pig manure that (2) will become thoroughly decomposed and the dish dregs of rice ratio of 6: 4 in mass ratio mixes, again the fermented liquid of above-mentioned NJAUYL-25 is inoculated in this mixture and carries out solid fermentation by the amount of 50L/ ton, in fermenting process, turning every day is 1 time, temperature is no more than 50 ℃, fermented 7 days, after fermentation ends, the quantity of growth-promoting bacterial strain NJAUYL-25 reaches 1 * 10 8More than cfu/g; Obtain the pig manure of growth-promoting bacterial strain NJAUYL-25 and the solids mixing microbial inoculum of dish dregs of rice microorganism enzymolysis mixture, finally under temperature is no more than the condition of 60 ℃, the water content of microbial organic fertilizer is evaporated to below 30%, packing is dispatched from the factory and is the microbial organic fertilizer that can promote the banana plant growth; Wherein:
The pig manure germination index become thoroughly decomposed used is more than 98%, quality of organic matter ratio content >=45%, and the organonitrogen quality is 1.2-2.0% than content, water content mass ratio 25-30%;
The microorganism enzymolysis preparation method of mixture of the dish dregs of rice is, germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid that is CGMCC NO.1543 by preserving number joins in dish dregs of rice raw material, regulate water content 55-65% and pH6.0-7.5, carry out open solid fermentation, when rising to 50 ℃, leavening temperature starts immediately turning, turning every day later, make to pile temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation starts, the pH of stacking yard can constantly rise, during each turning, regulate water content and pH with containing the acid solution body, make them maintain respectively 55-65% and 6.0-7.0, when stopping, fermentation with containing the acid solution body, sprays again, until stacking yard pH is adjusted into 5.0, carry out again cryodrying or natural airing, be the microorganism enzymolysis mixture of the dish dregs of rice.
4. the application of the described microorganism organic fertilizer of claim 2 or 3 in promoting Banana Growth.
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CN109251097A (en) * 2018-10-26 2019-01-22 湖北源清生物科技股份有限公司 A kind of biology compound organic fertilizer
CN109265246A (en) * 2018-10-26 2019-01-25 湖北源清生物科技股份有限公司 A kind of disease resistance biological organic fertilizer
CN109897806A (en) * 2019-04-15 2019-06-18 南京农业大学 One plant of bacillus megaterium for promoting the growth of crop tolerance to salt alkali and salt-soda soil microbial fertilizer special and application
CN109897806B (en) * 2019-04-15 2022-05-10 南京农业大学 Bacillus megaterium for promoting saline-alkali tolerant growth of crops, special microbial fertilizer for saline-alkali soil and application
CN116218741A (en) * 2023-04-04 2023-06-06 云南省农业科学院农业环境资源研究所 Stenotrophomonas maltophilia Sz-2 and application thereof
CN116218741B (en) * 2023-04-04 2023-09-26 云南省农业科学院农业环境资源研究所 Stenotrophomonas maltophilia Sz-2 and application thereof

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Application publication date: 20131127