CN101691549B - Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof - Google Patents
Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer produced by the same, belonging to the technique of agricultural intensive production. In the invention, two strains of antagonistic bacteria which have remarkable antagonistic effect on the pathogenic fungi of continuous cropping melon blastdisease (bacillus polymyxa NJY10 and bacillus subtilis NJY-IVI) are obtained by separation; the antagonistic bacteria is mixed with the organic fertilizer to be prepared into microorganism organic fertilizer, wherein the fertilizer comprises more than 1*108 antagonistic bacteria per gram, 4-5% of total nitrogen content, 6-10% of total nitrogen, phosphorous and potassium nutrient and 30-35% of organic matter content. Experiments show that after being fertilized into the soil, the microorganism organic fertilizer can lead the antagonistic bacteria to reproduce rapidly and form a superior group in the soil, the biological prevention and cure rate towards the melon blast disease in the continuous cropping soil with serious melon blast disease can reach over 80%; and in the soil without forming continuous cropping impediment, long-term use of the microorganism organic fertilizer of the invention can prevent and cure the occurrence of melon blast disease substantially.
Description
One, technical field
The present invention relates to prevent and treat the antagonism bacterium and the microorganism organic fertilizer thereof of continuous cropping melon blast disease, belong to the intensive agriculture production technology, be exclusively used in and overcome and eliminate the continuous cropping melon blast disease problem.
Two, technical background
Muskmelon all has cultivation all over the world, be the whole world one of ten big fruit, is that people generally like the fruit of eating raw of food all over the world.Distribution range to about 23 ° in south latitude, spreads all over five continents, wherein Zhong Guo cultivated area maximum from about 65 ° of north latitude.All there is extensive plantation various places, China north and south, and wherein the Northwest such as Xinjiang, Gansu is traditional producing region of thin skin muskmelon.Muskmelon disease and pest particularly muskmelon blight always is the major issue that influences its production, and protection ground facility planting type is progressively promoted since the nineties, and it is serious that the muskmelon blight more becomes.Continuous cropping melon blast disease is to be caused by pathogenic fungi (Fusarium oxysporum), just begins to occur when 2 batches of continuous croppings, and 3 batches of continuous croppings are serious all the more later on.Traditional chemical prevention and control method can cause negative impact to the environment and the mankind.Our result of study shows that the key issue of succession crop blight is not nutrient deficiency or imbalance, neither the soil secondary salinization problem, but the micro-flora in the soil changes and the intoxicating problem of secondary metabolite.
On the other hand, the feces of livestock and poultry behind crop material on-site incineration, the large-scale cultivations such as paddy rice, wheat, corn, rape has been thrown aside etc. not only severe contamination everywhere environment, also greatly having wasted can be as the raw material of fertilizer and biological organic fertilizer product; A large amount of nutrient resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened the ability of China's agricultural sustainable development significantly.The nutritive element how will take away because of crop harvesting from soil is returned in the soil to greatest extent and goes, and unique approach is made the organic commercial fertilizer material with these solid organic castoffs exactly and is manured into soil.If through the synthetic high-grade organic compost of thermophilic fermentation, as the carrier of function yeast, made microorganism organic fertilizer definite functions will have good application prospects again with these solid organic castoffs.
Three, summary of the invention
1. technical problem
The objective of the invention is to develop a kind of microorganism organic fertilizer that can prevent and treat continuous cropping melon blast disease, the efficient that overcomes or eliminate continuous cropping melon blast disease reaches more than 80%, make a large amount of industrialized agriculture soil and open country muskmelon continuous cropping obstacle soil obtain biological restoration, guarantee agriculture the shaping up that intensify.
2. technical scheme
Two strains can overcome or eliminate the antagonism bacteria NJY 10 and the NJY-IVI of continuous cropping melon blast disease, belong to bacillus polymyxa (Paenibacillus polymyxa) and subtilis (Bacillus subtilis) respectively, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 9th, 2009, the preserving number of NJY10 is CGMCCNO.3178, and the preserving number of NJY-IVI is CGMCC NO.3179.
The main biological characteristics of NJY10 is: Gram-positive (G
+), cell is shaft-like, hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, methyl red test feminine gender, Mierocrystalline cellulose decomposes negative, gelatin liquefaction positive, the nitrate reduction positive, indole test feminine gender, can utilize Citrate trianion, can produce ammonia, glucose fermentation produces sour aerogenesis, and xylose-fermenting, L-arabinose, seminose only produce acid.
The main biological characteristics of NJY-IVI is Gram-positive (G
+), cell is shaft-like, hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, methyl red test feminine gender, Mierocrystalline cellulose decomposes negative, gelatin liquefaction positive, the nitrate reduction positive, the indole test positive, can utilize Citrate trianion, can produce ammonia, glucose fermentation produces sour aerogenesis, and xylose-fermenting, L-arabinose, seminose only produce acid.
With above-mentioned can overcome or eliminate in the microorganism organic fertilizer that continuous cropping melon blast disease antagonism bacterium produced contain 1 * 10
8Antagonism bacteria NJY 10 or NJY-IVI that individual/g is above, or antagonism bacteria NJY 10 and NJY-IVI, total nitrogen content are that 4～5% (they being organonitrogen 90% or more), total nitrogen phosphorus potassium nutrition are 6～10%, organic content is 30～35%.Its production method comprises:
1) antagonism bacteria NJY 10 and NJY-IVI are inoculated into the PDA nutrient solution respectively, carry out liquid fermenting production respectively, the condition of fermentative production is: pH growth scope nature, 30～38 ℃ of culture temperature scopes, stirring velocity is 180～300 rev/mins, the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount 〉=5 * 10 in the fermented liquid
9Individual/ml.Used PDA nutrient solution compound method is, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling, add the 20g common sucrose after filtering in the filtrate, be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
2) with the antagonism bacterium with become thoroughly decomposed after pig manure and dish dregs of rice microorganism enzymolysis mixture carry out solid fermentation respectively, add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, general fermentation finished after 5 days, made the antagonism bacterial content reach 1 * 10
8Individual/as more than the g, to obtain the pig manure solid fungicide and the dish dregs of rice microorganism enzymolysis mixture solid microbial inoculum of antagonism bacteria NJY 10, obtain two kinds of solid fungicides of antagonism bacterium NJY-IVI equally.Pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=35%, and the organonitrogen quality is 1.2-2% than content, water content mass ratio 25-30%.
The microorganism enzymolysis preparation method of mixture of its Chinese food dregs of rice is, with preserving number is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material, regulate water content 55-65% and pH 6.0-7.5, carry out open solid fermentation, treat to begin immediately when leavening temperature rises to 50 ℃ turning, turning every day later on, make the heap temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation begins, the pH of stacking yard can constantly rise, during each turning with containing acid solution body adjusting water content and pH, make them maintain 55-65% and 6.0-7.0 respectively, spray with containing the acid solution body again when fermentation stops, be adjusted into about 5.0 until stacking yard pH, carry out cryodrying or natural airing again, be the microorganism enzymolysis mixture of the dish dregs of rice.
3) microorganism enzymolysis mixture two classes of pressing the pig manure and the dish dregs of rice are mixed antagonism bacteria NJY 10 solid fungicide and NJY-IVI solid fungicide with volume ratio respectively at 1: 1, obtain the solid mix bacterium agent of pig manure and the solid mix bacterium agent of dish dregs of rice microorganism enzymolysis mixture.
4) again with the single microbial inoculum of solid of pig manure, mix bacterium agent by volume 50-70% respectively with the single microbial inoculum of solid of dish dregs of rice microorganism enzymolysis mixture, mix bacterium agent is the ratio thorough mixing of 30-50% by volume, and after-ripening 2-3 days, turning is 2 times in the after-ripening process, at last the water content that is no more than under 60 ℃ the condition microbial organic fertilizer in temperature is evaporated to below 30%, obtain to prevent and treat the antagonism bacteria NJY 10 of continuous cropping melon blast disease respectively, the single bacteria microorganism fertilizer of NJY-IVI and antagonism bacteria NJY 10 and NJY-IVI mixing microorganisms fertilizer, packing dispatch from the factory and are the microorganism organic fertilizer of control continuous cropping melon blast disease.
The mentioned microorganism organic fertilizer can be exclusively used in the control continuous cropping melon blast disease.
3. beneficial effect
A kind of microorganism organic fertilizer and production method thereof that can overcome or eliminate continuous cropping melon blast disease of the present invention, utilize pig manure and dish dregs of rice microorganism enzymolysis aminoacid mixture, make microorganism organic fertilizer again with after antimicrobial fluid short of money is mixed, its product is compared with product in the market has following advantage:
1) contain in the fertilizer product the efficient bacterial strain of specificity that suppresses the growth of muskmelon blight fungal pathogens (NJY10, NJY-IVI), it suppresses effect highly significant, the content of this efficient bacterial strain reaches 1 * 10 in the fertilizer
8Individual/more than the g, energy formation advantage group after being manured into soil, and under in soil, surviving.
2) contain rich soluble organic carbon and organonitrogen in the fertilizer product, can provide very superior growing environment, greatly improved the ability that function is grown and brought into play to antagonism bacterium deciding in soil to function yeast.
3) product organophosphorus of the present invention and organonitrogen content are high especially, all reach 4%.One of this products material is a pig manure, and total phosphorous is very high, and biological effectiveness is high especially, need not to add any phosphatization fertilizer that contains in the microorganism organic fertilizer product of making, and uses to make behind this product crop spend phosphorus in seedling stage plain critical porion smoothly.By contrast, it is relatively poor to contain the phosphatization fertilizer artifact validity that is manured into soil; Organonitrogen can reach more than 4% (most of be amino acid and micromolecule polypeptide) in this product, has guaranteed the continual and steady supply of fertilizer nitrogen, make plant plant growth and anti-adversity ability strengthens greatly.
4) test-results shows, after using this product on the soil of continuous cropping muskmelon, can significantly reduce the sickness rate of blight, and the biological control rate reaches more than 80%, the continuous soil of using this fertilizer in 3 years, and the biological control rate of continuous cropping melon blast disease is higher.
Four, description of drawings
Fig. 1 antagonistic strain inhibition zone
Five, embodiment
(1) separation of bacterial strain and evaluation
Gather the plant and the surrounding soil that continuous cropping obstacle have taken place and serious muskmelon blight occurred, the sample cryopreservation adopts Kelman ' s TTC Agar selective medium bacterial isolate bacterium.Be indicator with muskmelon blight pathogenic bacteria then, separate the antagonism bacterium in the healthy plant rhizosphere soil from the piece of continuous cropping melon blast disease morbidity field.Finally sift out efficient antagonistic strain NJY10 again by pot experiment and field experiment again, NJY-IVI identifies to belong to and plants, and bacterial classification is-70 ℃ of preservations in 30% glycerine.Antagonistic strain NJY10, the NJY-IVI inhibition zone as shown in Figure 1.
Antagonism bacteria NJY 10 belongs to Paenibacillus polymyxa (Paenibacillus polymyxa), and its main biological characteristics is: Gram-positive (G
+), cell is shaft-like, hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, methyl red test feminine gender, Mierocrystalline cellulose decomposes negative, gelatin liquefaction positive, the nitrate reduction positive, indole test feminine gender, can utilize Citrate trianion, can produce ammonia, glucose fermentation produces sour aerogenesis, and xylose-fermenting, L-arabinose, seminose only produce acid.
Antagonism bacterium NJY-IVI belongs to subtilis (Bacillus subtilis), and its main biological characteristics is: Gram-positive (G
+), cell is shaft-like, hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, methyl red test feminine gender, Mierocrystalline cellulose decomposes negative, gelatin liquefaction positive, the nitrate reduction positive, the indole test positive, can utilize Citrate trianion, can produce ammonia, glucose fermentation produces sour aerogenesis, and xylose-fermenting, L-arabinose, seminose only produce acid.
(2) microbial inoculum production
1) antagonism bacteria NJY 10 and NJY-IVI are inoculated into the PDA nutrient solution respectively, carry out liquid fermenting production respectively, the condition of fermentative production is: pH growth scope nature, 30～38 ℃ of culture temperature scopes, stirring velocity is 180～300 rev/mins, the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount 〉=5 * 10 in the fermented liquid
9Individual/ml.Used PDA nutrient solution compound method is, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling, add the 20g common sucrose after filtering in the filtrate, be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min.
2) with the antagonism bacterium with become thoroughly decomposed after pig manure and dish dregs of rice microorganism enzymolysis mixture carry out solid fermentation respectively, add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, general fermentation finished after 5 days, made the antagonism bacterial content reach 1 * 10
8Individual/as more than the g, to obtain the pig manure solid fungicide and the dish dregs of rice microorganism enzymolysis mixture solid microbial inoculum of antagonism bacteria NJY 10, obtain two kinds of solid fungicides of antagonism bacterium NJY-IVI equally.Pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=35%, and the organonitrogen quality is 1.2-2% than content, water content mass ratio 25-30%.
The microorganism enzymolysis preparation method of mixture of its Chinese food dregs of rice is (public, see patent of invention ZL200610086126.0, a kind of organism preparation method of agricultural amino acid and fertilizer product thereof), germ oligotrophy unit cell (Stenotrophomonas maltophilia) bacterial strain 37-1 fermented liquid is joined in the dish dregs of rice raw material, regulate water content 55-65% and pH 6.0-7.5, carry out open solid fermentation, treat to begin immediately when leavening temperature rises to 50 ℃ turning, turning every day later on, make the heap temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation begins, the pH of stacking yard can constantly rise, during each turning with containing acid solution body adjusting water content and pH, make them maintain 55-65% and 6.0-7.0 respectively, spray with containing the acid solution body again when fermentation stops, be adjusted into about 5.0 until stacking yard pH, carry out cryodrying or natural airing again, be the microorganism enzymolysis mixture (containing amino acid whose mixing material) of the dish dregs of rice.
3) microorganism enzymolysis mixture two classes of pressing the pig manure and the dish dregs of rice are mixed antagonism bacteria NJY 10 solid fungicide and NJY-IVI solid fungicide with volume ratio respectively at 1: 1, obtain the solid mix bacterium agent of pig manure and the solid mix bacterium agent of dish dregs of rice microorganism enzymolysis mixture;
4) will contain single bacteria NJY 10 again, single bacterium NJY-IVI, the pig manure solid fungicide of mixed bacterium NJY10 and NJY-IVI by volume 50-70% respectively with contain single bacteria NJY 10, single bacterium NJY-IVI, the dish dregs of rice microorganism enzymolysis mixture solid microbial inoculum of mixed bacterium NJY10 and NJY-IVI is 30% ratio thorough mixing by volume, and after-ripening 2-3 days, turning is 2 times in the after-ripening process, at last the water content that is no more than under 60 ℃ the condition microbial organic fertilizer in temperature is evaporated to below 30%, and single bacteria microorganism fertilizer that acquisition can prevent and treat continuous cropping melon blast disease (contains antagonism bacteria NJY 10 respectively, NJY-IVI), mixing microorganisms fertilizer (containing antagonism bacteria NJY 10 and NJY-IVI).
Mixing microorganisms fertilizer (microorganism organic fertilizer of control continuous cropping melon blast disease) with above-mentioned antagonism bacterium is produced wherein contains 1 * 10
8Antagonism bacteria NJY 10 or NJY-IVI that individual/g is above, or antagonism bacteria NJY 10 and NJY-IVI, total nitrogen content are that 4～5% (they being organonitrogen 90% or more), total nitrogen phosphorus potassium nutrition are 6～10%, organic content is 30～35%.
(3) pot experiment
Choosing healthy rice soil sieves as growing seedlings soil.Nutrition pot adopts the disposable plastic water tumbler, and every alms bowl is adorned native 300g, and the microbial fertilizer amount of application is a 2g/100g soil.
The every basin dress of big basin 10kg cause of disease soil, cause of disease soil picks up from Anhui and the county's muskmelon continuous cropping booth (the blight sickness rate reaches more than 70%).The microbial organic fertilizer amount of application is 0.5% in the big basin, and promptly every basin adds the 50g microbial organic fertilizer, and fertilizer and cause of disease soil thorough mixing are even.Treat that the muskmelon seedling is transplanted into when growing to 3-4 sheet true leaf in the big basin alms bowl that muskmelon blight pathogenic bacteria sick soil is housed.8 processing are set altogether, and every processing repeats 10 times.This pot experiment and in March, 2008 to November planted for three seasons continuously in Yixing, Jiangsu Province engineering center greenhouse.
This pot experiment is handled as follows:
1, nutrition pot, big basin are all used equivalent chemical fertilizer (i.e. contrast)
2, nutrition pot is used common fertilizer, and big basin is executed the equivalent chemical fertilizer
3, nutrition pot is executed the single bacteria microorganism fertilizer of NJY10, and big basin is executed the equivalent chemical fertilizer
4, nutrition pot is executed the single bacteria microorganism fertilizer of NJY-IVI, and big basin is executed the equivalent chemical fertilizer
5, nutrition pot is executed NJY10 and NJY-IVI mixed bacterium microbial organic fertilizer, and big basin is executed the equivalent chemical fertilizer
6, nutrition pot is executed the equivalent chemical fertilizer, and big basin is executed NJY10 and NJY-IVI mixed bacterium microbial organic fertilizer
7, nutrition pot is executed common fertilizer, and big basin is executed NJY10 and NJY-IVI mixed bacterium microbial organic fertilizer
8, nutrition pot, big basin are all executed NJY10 and NJY-IVI mixed bacterium microbial organic fertilizer
Discovery blight disease plant is observed the plant incidence every day when transplanting 16 days, the record sickness rate, and gather plant sample and the soil sample respectively handled and take back lab analysis mensuration.
The result shows (table 1), handle 1,2,3,4,5,6,7,8 muskmelon plant sickness rate are respectively 90%, 70%, 20%, 20%, 15%, 70%, 70%, 15%, no matter illustrating in the nutrition pot or using the microbial organic fertilizer of being made by the antagonism bacterium in the big basin separately to make the sickness rate of muskmelon blight descend, but use the effect of microbial organic fertilizer more obvious in the nutrition pot, use single bacteria microorganism fertilizer of NJY-IVI or the single bacteria microorganism fertilizer of NJY10 in the nutrition pot, control rate to the muskmelon blight all reaches 77.8%, and uses NJY10 in the nutrition pot, NJY-IVI mixed bacterium microbial organic fertilizer can reach 80% to the control rate to the muskmelon blight.Use all in the nutrition pot and in the big basin that the control rate to the muskmelon blight also reaches 80% behind the mixed bacterium microorganism organic fertilizer.It is best to control muskmelon blight effect to use mixed bacterium.
Nutrition pot or nutrition pot and big basin are all used the mixed bacterium microbial organic fertilizer can both obviously increase the plant biomass.Handling 1 (the big basin of nutrition pot is all used chemical fertilizer) plant biomass is the 10.4g/ strain, (nutrition pot is executed NJY10 and NJY-IVI mixed bacterium microbial organic fertilizer and handle 5, big basin is to fertilize), handle 8 (nutrition pot, big basin are all executed NJY10 and NJY-IVI mixed bacterium microbial organic fertilizer) plant biomass and be respectively 113.9g/ strain, 183.3g/ strain, the plant biomass has increased by 9.9,16.6 times respectively.Simultaneously, nutrition pot is used the single bacteria microorganism fertilizer of NJY10, NJY-IVI also can both obviously increase the plant biomass, and the plant biomass is respectively 114.73g/ strain, 93.68g/ strain, and the plant biomass has increased by 10 times respectively, 8 times.The result shows: nutrition pot, that big basin is all used mixed bacterium microbial organic fertilizer effect of increasing production is best.
Utilize the Fusarium oxysporum selective medium that pathogenic bacteria Fusarium oxysporum in muskmelon plants stems, root system, rhizosphere, the rhizosphere soil is counted (table 2,3), the result shows that plants stems when not using microbial organic fertilizer, root system, rhizosphere pathogenic bacteria quantity reach 10 respectively
4, 10
5More than, the plant sickness rate reaches more than 90%; And after nutrition pot used microbial organic fertilizer, the quantity of pathogenic bacteria in plant and soil was all 10
3, 10
4On the level, this shows that using microbial organic fertilizer can significantly reduce the quantity of pathogenic bacteria in soil, the plant, thereby improves the population structure of microorganism in the continuous cropping soil, plays the biological and ecological methods to prevent plant disease, pests, and erosion effect of control continuous cropping melon blast disease.
Table 1 muskmelon basin alms bowl test sickness rate and plant biomass
Table 2 plants stems, root system pathogenic bacteria quantity
Table 3 a table soil, the native pathogenic bacteria quantity of rhizosphere
Claims (6)
1. can prevent and treat the antagonism bacterium of continuous cropping melon blast disease, comprise bacterial strain NJY10 and bacterial strain NJY-IVI, belong to Paenibacillus polymyxa (Paenibacillus polymyxa) and subtilis (Bacillus subtilis) respectively, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 9th, 2009, the preserving number of NJY10 is CGMCC NO.3178, and the preserving number of NJY-IVI is CGMCC NO.3179;
The main biological characteristics of NJY10 is: Gram-positive G
+Cell is shaft-like, hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, methyl red test feminine gender, Mierocrystalline cellulose decomposes negative, gelatin liquefaction positive, the nitrate reduction positive, indole test feminine gender, can utilize Citrate trianion, can produce ammonia, glucose fermentation produces sour aerogenesis, and xylose-fermenting, L-arabinose, seminose only produce acid;
The main biological characteristics of NJY-IVI is Gram-positive G
+Cell is shaft-like, hydrogen peroxide enzyme positive, the starch hydrolysis positive, V.P. reacting positive, methyl red test feminine gender, Mierocrystalline cellulose decomposes negative, gelatin liquefaction positive, the nitrate reduction positive, the indole test positive, can utilize Citrate trianion, can produce ammonia, glucose fermentation produces sour aerogenesis, and xylose-fermenting, L-arabinose, seminose only produce acid.
2. the microorganism organic fertilizer of using the described antagonism bacterium of claim 1 to produce.
3. microorganism organic fertilizer according to claim 2 is characterized in that: contain 1 * 10 in this fertilizer
8Antagonism bacteria NJY 10 that individual/g is above and NJY-IVI, full nitrogen quality than content be 4～5%, mass ratio is that organonitrogen, total nitrogen phosphorus potassium nutrition mass ratio are 6～10%, the quality of organic matter ratio content is 30～35% 90% or more in the full nitrogen.
4. the production method of claim 2 or 3 described microorganism organic fertilizers comprises:
1) antagonism bacteria NJY 10 and NJY-IVI are inoculated into the PDA nutrient solution respectively, carry out liquid fermenting production respectively, the condition of fermentative production is: 30～38 ℃ of culture temperature scopes, stirring velocity are 180～300 rev/mins, the fermentation middle and later periods forms gemma, makes to contain bacterium or gemma amount 〉=5 * 10 in the fermented liquid
9Individual/ml, used PDA nutrient solution compound method is, is example with preparation 1L substratum: be cut into small pieces to be put in the water after peeling with the 200g potato and boil, boil 30min after the boiling, add the 20g common sucrose after filtering in the filtrate, be settled to 1000ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20min;
2) with the antagonism bacterium with become thoroughly decomposed after pig manure and the microorganism enzymolysis mixture of the dish dregs of rice carry out solid fermentation respectively, add antimicrobial fluid 50L short of money in the SOLID ORGANIC material per ton, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, general fermentation finished after 5 days, made the antagonism bacterial content reach 1 * 10
8Individual/as more than the g, to obtain the pig manure solid fungicide of antagonism bacteria NJY 10 and the dish dregs of rice microorganism enzymolysis mixture solid microbial inoculum of NJY10, obtain two kinds of solid fungicides of antagonism bacterium NJY-IVI equally;
The microorganism enzymolysis preparation method of mixture of its Chinese food dregs of rice is, with preserving number is that germ oligotrophy unit cell (Stenotrophomonas maltophilia) the bacterial strain 37-1 fermented liquid of CGMCC NO.1543 joins in the dish dregs of rice raw material, regulate water content 55-65% and pH 6.0-7.5, carry out open solid fermentation, treat to begin immediately when leavening temperature rises to 50 ℃ turning, turning every day later on, make the heap temperature maintain all the time 35-50 ℃ totally 5 days, after solid fermentation begins, the pH of stacking yard can constantly rise, during each turning with containing acid solution body adjusting water content and pH, make them maintain 55-65% and 6.0-7.0 respectively, spray with containing the acid solution body again when fermentation stops, be adjusted into about 5.0 until stacking yard pH, carry out cryodrying or natural airing again, be the microorganism enzymolysis mixture of the dish dregs of rice;
3) microorganism enzymolysis mixture two classes of pressing the pig manure and the dish dregs of rice are mixed antagonism bacteria NJY 10 solid fungicide and NJY-IVI solid fungicide with volume ratio respectively at 1: 1, obtain the solid mix bacterium agent of pig manure and the solid mix bacterium agent of dish dregs of rice microorganism enzymolysis mixture;
4) again with the solid mix bacterium agent of the pig manure solid mix bacterium agent ratio thorough mixing of 30-50% by volume of 50-70% and dish dregs of rice microorganism enzymolysis mixture by volume, and after-ripening 2-3 days, turning is 2 times in the after-ripening process, at last the water content that is no more than under 60 ℃ the condition microbial organic fertilizer in temperature is evaporated to below 30%, acquisition can prevent and treat the antagonism bacteria NJY 10 and the NJY-IVI mixing microorganisms fertilizer of continuous cropping melon blast disease, and packing is dispatched from the factory and is the microorganism organic fertilizer of control continuous cropping melon blast disease.
5. according to the production method of the described microorganism organic fertilizer of claim 4, step 2 wherein) the pig manure germination index after used the becoming thoroughly decomposed is more than 98%, quality of organic matter ratio content 〉=35%, the organonitrogen quality is 1.2-2% than content, water content mass ratio 25-30%.
6. claim 2 or the 3 described microorganism organic fertilizers application in the control continuous cropping melon blast disease.
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