CN103232272B - A kind of compound organic microbial fertilizer and manufacture method thereof - Google Patents
A kind of compound organic microbial fertilizer and manufacture method thereof Download PDFInfo
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- CN103232272B CN103232272B CN201310167920.8A CN201310167920A CN103232272B CN 103232272 B CN103232272 B CN 103232272B CN 201310167920 A CN201310167920 A CN 201310167920A CN 103232272 B CN103232272 B CN 103232272B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of compound organic microbial fertilizer and manufacture method thereof, humic acid 5-30 weight part, agricultural crop straw charcoal 10-30 weight part or rice husk meal 20-30 weight part, animal excrement 10-30 weight part; Agricultural crop straw charcoal or rice husk meal are crushed to 80-100 order, and humic acid is crushed to 80-100 order, the sterilizing of animal excrement heating, drying, by for subsequent use as solid support material after said ratio mixing; Get 3-5 weight part mixed biologic preparation, with solid support material 800-900 weight part mixing and stirring for subsequent use, adjustment humidity 20-30%, enters to store pond sealed fermenting, temperature 10-50 DEG C, and fermentation 48-60 hour, takes out water content less than 18% granulation below 40 DEG C.This bacterial manure suppresses soil-borne disease and growth promoting effects effect significantly to having of the continuous cropping fields such as facility strawberry, and amount of increase in production reaches 10-30%, and early flowering result, amount of increase in production reaches 10-30%.
Description
Technical field
The present invention relates to the production technique of a kind of fertilizer and fertilizer, i.e. a kind of compound organic microbial fertilizer and manufacture method.
Background technology
Biological in effect agriculturally gradually be familiar with by people.Now existing more than 70 countries produce, apply and promote microbial fertilizer in the world, and current Ye You250Jia enterprise of China produces about few hundred thousand tonnes of microbial fertilizer per year and is applied to production.Though this can not compare with consumption with the fertilizer production same period, indeed start to play a role in agriculture production, achieve certain economic benefit and social effect, begun to take shape the regular suitability for industrialized production stage.The needs going deep into and apply along with research constantly expand the exploitation of new variety, and microbial fertilizer is now formed (1) is transformed to Non-legume plants fertilizer by leguminous crop Inoculant; (2) transformed to composite biological fertilizer by single Inoculant; (3) transformed to composite bacteria by single culture; (4) by simple function to Multifunctional Conversion; (5) by with producing to there being brood cell's bacterial classification to produce the trend such as conversion without brood cell's bacterial classification.Moreover, over nearly 20 years, many countries more recognize that microbial fertilizer is as the microbial preparation of living, its beneficial microorganism quantity and vital movement whether vigorous be the key of quality, be one of key of effect quality.For this reason, now existing many countries establish industry or national standard and corresponding mechanism to check the quality of the products.China has also formulated Ministry of Agriculture's standard and has set up microbial quality inspection center, and formally carries out product registration, detection to microorganism fertilizer material products in 1996 and provide the work such as production licence.
In recent years, China increased research dynamics under microbial fertilizer development situation.Believe the progress along with science, research and the needs of production development and the perfect of supervisory system, microbial fertilizer one develops surely healthy and orderlyly, and be its due effect of performance that gets agricultural profit, prospect will be boundless.
Microbial fertilizer is live body fertilizer, and a large amount of beneficial microorganism vital movements that its effect mainly contains by it have been come.Only have under these beneficial microorganisms are in vigorous breeding and metabolic situation, Substance Transformation and beneficial metabolic product could constantly be formed.Therefore, whether beneficial microorganism kind in microbial fertilizer, vital movement vigorous is the basis of its validity, and unlike other fertilizer be by the form of the principal elements such as nitrogen, phosphorus, potassium and how many based on.Just because of microbial fertilizer is preparation of living, so its fertilizer efficiency and number of viable, intensity and ambient environmental conditions closely related, comprising temperature, moisture, potential of hydrogen, nutritional condition and formerly live in indigenous microorganism repulsive interaction in soil and have certain influence, therefore will be noted when applying.
Along with improving constantly of living standards of the people, the especially requirement that improves quality of life of people, all comes production safety, non-harmful green food in develop actively green agriculture (sustainable agriculture) both at home and abroad.Produce requirement in green food process and (or limitation uses) chemical fertilizer, chemical pesticide and other chemical substance or need not be used as far as possible less.It requires that first fertilizer must be protected and promote subject to grow and improve quality; Secondly subject is not caused to produce and accumulation objectionable impurities; Three is have no adverse effects to ecotope.Microbial fertilizer meets above three principles substantially.In recent years, multiple-microorganism fertilizer is made with the bacterial classification with specific function by China, not only can relax or reduce pollution of agricultural products, and can improve the quality of agricultural-food.
In microbial fertilizer, beneficial microorganism can produce glucide, can improve soil aggregate, strengthens the physicals of soil and reduces the loss of soil particle, under certain conditions, can also participate in soil ulmin and be formed.So use microbial fertilizer can improve soil physical property, be conducive to increasing soil fertility.
Microbial-bacterial fertilizer is the agricultural fertilizer utilizing biotechnology production beneficial microorganism to make together with suitable carrier.Bio-bacterial manure provides this nutrient by the metabolism of microorganism for crop, and Crop Improvement living environment, even participates in Some Physiological Processes of Crops, strengthens resistance, improves product amount and quality.And pollution-free, without residual hazard, have a good application prospect., it is single that current bio-bacterial manure also exists bacterial classification, and problems such as acting on simple, involve great expense, the large transport of consumption is inconvenient, limits penetration and promotion.Visible in the patent literature, most bio-bacterial manure only has a bacterial classification.Some bacterial manure bacterial classifications are a lot, but support material is difficult to.Such as, many take coal gangue as the bacterial manure of carbon source carrier, and its supplemental nitrogen source carrier is but limited with wheat bran, chicken manure etc., and source seldom, is difficult to the needs meeting agriculture production.
Summary of the invention
The object of the invention is to provide a kind of compound organic microbial fertilizer and manufacture method thereof for the deficiencies in the prior art.
Above-mentioned purpose is realized by following technical scheme:
A manufacture method for compound organic microbial fertilizer, humic acid 5-30 weight part, agricultural crop straw charcoal 10-30 weight part or rice husk meal 20-30 weight part, animal excrement 10-30 weight part; Agricultural crop straw charcoal or rice husk meal are crushed to 80-100 order, and humic acid is crushed to 80-100 order, the sterilizing of animal excrement heating, drying, by for subsequent use as solid support material after said ratio mixing; Get 3-5 weight part mixed biologic preparation, with solid support material 800-900 weight part mixing and stirring for subsequent use, adjustment humidity 20-30%, enters to store pond sealed fermenting, temperature 10-50 DEG C, and fermentation 48-60 hour, takes out water content less than 18% granulation below 40 DEG C.
Described manufacture method, the preparation method of described mixed biologic preparation is: the peat composed of rotten mosses or other carriers that the fermented liquid of fermentation of bacillus subtilis liquid and bacillus polymyxa are added respectively 3 times of fermented liquid weight, uniform adsorption, then dry at 60 DEG C, and measure water content in good time, make water content control between 3-5%, be prepared into the microbial inoculum containing subtilis and Acarasiales genus bacillus living spores respectively; By saccharomycetic the fermented liquid peat composed of rotten mosses of 3 times of fermented liquid weight or other carriers, uniform adsorption, then dries, and measures water content in good time, make water content control between 8-10% at 40 DEG C, is prepared into the microbial inoculum containing spore of living; Then get above-mentioned three kinds of microbial inoculums mixing of equivalent weight respectively, make containing subtilis, bacillus polymyxa and saccharomycetic mixed biologic preparation.
Adopt the bacterial manure that technique scheme is made, the experiment proved that, this bacterial manure suppresses soil-borne disease and growth promoting effects effect significantly to having of the continuous cropping fields such as facility strawberry, and amount of increase in production reaches 10-30%, early flowering result, amount of increase in production reaches 10-30%, the use of agricultural chemicals, chemical fertilizer, hormone can be reduced, avoid chemical pollution, improvement soil property, improve product quality, make product reach green or organic standard.Particularly have employed rice husk meal and animal excrement crop carrier, reduce further the cost of bacterial manure, the popularization and application for bacterial manure opens new approach.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1: get humic acid (total humic acid is greater than 40%) 5-10 weight part by weight, agricultural crop straw charcoal is (by agricultural crop straw through incomplete combustion, at the method for cooking with charcoal, stalk is made charcoal) 10-20 weight part, animal excrement 20-30 weight part.Agricultural crop straw powdered carbon is broken to 80-100 order, and humic acid is crushed to 80-100 order, the sterilizing of animal excrement heating, drying, by for subsequent use as solid support material after said ratio mixing.Get 3-5 weight part mixed biologic preparation, with solid support material 800-900 weight part mixing and stirring for subsequent use, adjustment humidity 20-30%, enters to store pond sealed fermenting, temperature 10-50 DEG C, and fermentation 48-60 hour, takes out water content less than 18% granulation below 40 DEG C.
Mixed biologic formulation process and method:
1. strains tested: subtilis, bacillus polymyxa, yeast.Subtilis is bought in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCCNo.6314, Paenibacillus polymyxa (Bacilluspolymyxa) is bought in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCCNO.1.224, and described yeast is commercially available conventional yeast saccharomyces cerevisiae.
2. for examination substratum LB substratum, PDA substratum.
3. the fermentation of subtilis and bacillus polymyxa
Respectively by the subtilis that activates on LB solid medium and bacillus polymyxa bacterial strain list bacterium colony, transfer in the volume that 5mLPDA liquid nutrient medium is housed be in the triangular flask of 20mL, in 30 DEG C, shaking culture 16h under 200rpm condition.By the nutrient solution (10mL) of upper step gained two bacterial strains, being all inoculated in the volume that 400mLPDA substratum is housed is in the triangular flask of 1000mL, in 30 DEG C, shaking culture 16h under 200rpm condition.The 400mL nutrient solution of gained is inoculated in be equipped with in the mechanical agitation type fermentor tank of 20LPDA substratum, fermentation condition is set as: dissolved oxygen 100%, stirring velocity 350rpm, leavening temperature 30 DEG C, fermentation time 48h, pH7.0-7.2 (making pH adjusting agent with 1MHCl and 1MNaOH).After fermentation is reached home, collect bacterium liquid for subsequent use.
4. saccharomycetic fermentation
By the yeast strain list bacterium colony activated on PDA solid medium, transfer in the volume that 5mLPDA liquid nutrient medium is housed be in the triangular flask of 20mL, in 25 DEG C, shaking culture 48h under 140rpm condition.By the nutrient solution (5mL) of upper step gained, being all inoculated in the volume that 400mLPDA substratum is housed is in the triangular flask of 1000mL, in 25 DEG C, shaking culture 48h under 140rpm condition.The 400mL nutrient solution of gained is inoculated in be equipped with in the mechanical agitation type fermentor tank of 20LPDA substratum, fermentation condition is set as: dissolved oxygen 80%, stirring velocity 200rpm, leavening temperature 25 DEG C, fermentation time 72h, pH7.0-7.2 (making pH adjusting agent with 1MHCl and 1MNaOH).After fermentation is reached home, collect bacterium liquid for subsequent use.
5. the preparation of microbial inoculum
The fermented liquid of fermentation of bacillus subtilis liquid and bacillus polymyxa is added respectively the peat composed of rotten mosses (or other carriers) uniform adsorption of 3 times of fermented liquid weight, then dry at 60 DEG C, and measure water content in good time, make water content control between 3-5%, be prepared into the microbial inoculum containing bacteria in viable gemma; By the peat composed of rotten mosses (or other carriers) uniform adsorption of saccharomycetic fermented liquid by 3 times of fermented liquid weight, then dry at 40 DEG C, and measure water content in good time, make water content control between 8-10%, be prepared into the microbial inoculum containing spore of living.Then get above-mentioned three kinds of microbial inoculums mixing of equivalent weight respectively, make containing subtilis, bacillus polymyxa and saccharomycetic mixed biologic preparation.
Embodiment 2: get humic acid (total humic acid is greater than 40%) 20-30 part by weight, rice husk meal 20-30 part and ight soil 20-30 part, rice husk meal is crushed to 80-100 order, humic acid is crushed to 80-100 order, the sterilizing of animal excrement heating, drying, by for subsequent use as solid support material after said ratio mixing.Get 3-5 part mixed biologic preparation (preparation method is with embodiment 1), with solid support material 800-900 part mixing and stirring for subsequent use, adjustment humidity 20-30%, enter to store pond sealed fermenting, temperature 10-50 DEG C, fermentation 45-60 hour, takes out water content less than 18% granulation below 40 DEG C.
Embodiment 3: get straw charcoal by weight (by agricultural crop straw through incomplete combustion, with the method for cooking of charcoal, stalk is made charcoal) 20-30 part, humic acid (total humic acid is greater than 40%) 20-30 part and animal excrement 10-20 part, stalk powdered carbon is broken to 80-100 order, humic acid is crushed to 80-100 order, the sterilizing of animal excrement heating, drying, by for subsequent use as solid support material after said ratio mixing.Get 3-5 part mixed biologic preparation, with solid support material 800-900 part mixing and stirring for subsequent use, adjustment humidity 20-30%, enters to store pond sealed fermenting, temperature 10-50 DEG C, and fermentation 45-60 hour, takes out water content less than 18% granulation below 40 DEG C.
Application example: the red cheek of strawberry cultivars, in peasant household's strawberry continuous cropping field, white rabbit town one, Jurong City, carry out in the strawberry greenhouse that former years, soil-borne disease weighed, within first 15 days, apply every mu of fermented rape cake 100 kilograms doing ridge, fermented manure every mu 2000 kilograms, N P and K Nitrogen, Phosphorus and Potassium 40 kilograms every mu, if test process: by embodiment 1, 2, 3 press mu consumption 10 kilograms respectively, in field planting first 7 days, face, ridge applies bio-bacterial manure of the present invention, another compound organic microbial fertilizer of not executing is for contrast, 4 process, each process 100 square metres, secondary repeats altogether, mu field planting 6000 strain, other control measures are consistent.First inflorescence fruiting period of strawberry investigation on January 20 verticillium diseased plant rate, grow, the situation such as number of results.
Table 1, bacteria agent are given birth to the disease-resistant of strawberry and promotion and are done sth. in advance the effect research of fruit picking
| Process | Verticillium diseased plant rate % | Preventive effect % | Plant height (cm) | Number of results on January 20 (individual) | Productive life |
| Embodiment 1 | 0.67 | 81％ | 21.56 | 15.65 | December 22 |
| Embodiment 2 | 1.04 | 70％ | 24.34 | 16.79 | December 19 |
| Embodiment 3 | 0.89 | 74％ | 23.54 | 15.86 | December 20 |
| Conventional fertilizer (CK) | 3，45 | ---- | 18.35 | 11.47 | December 28 |
Table one is found out, compound organic microbial fertilizer is compared with the contrast of not using, to verticillium preventive effect at 74-81%, plant height increases by 3.21 ~ 5.99cm, investigate first inflorescence number of results on December 20 and increase by 4.18 ~ 5.32, start to do sth. in advance 6 ~ 9 days collection period, therefore have obvious enhancing development and Early blooming and fruiting effect, can early yield be improved.
Within 2012, in white rabbit town, Jurong City, demonstrated in 50 mu, the continuous cropping field of facility strawberry, demonstrate compound organic microbial fertilizer further and there is significant suppression soil-borne disease and growth promoting effects effect, yield positive results more than 5 days ahead of time, amount of increase in production reaches 10-30%, the use of agricultural chemicals, chemical fertilizer, hormone can be reduced, avoid chemical pollution, improvement soil property, improve product quality, make product reach green or organic standard.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (2)
1. a manufacture method for compound organic microbial fertilizer, is characterized in that, humic acid 5-30 weight part, agricultural crop straw charcoal 10-30 weight part or rice husk meal 20-30 weight part, animal excrement 10-30 weight part, agricultural crop straw charcoal or rice husk meal are crushed to 80-100 order, and humic acid is crushed to 80-100 order, the sterilizing of animal excrement heating, drying, by for subsequent use as solid support material after said ratio mixing, get 3-5 weight part mixed biologic preparation, with solid support material 800-900 weight part mixing and stirring for subsequent use, adjustment humidity 20-30%, enter to store pond sealed fermenting, temperature 10-50 DEG C, fermentation 48-60 hour, take out water content less than 18% granulation below 40 DEG C, the preparation method of described mixed biologic preparation is: the peat composed of rotten mosses or other carriers that the fermented liquid of fermentation of bacillus subtilis liquid and bacillus polymyxa are added respectively 3 times of fermented liquid weight, uniform adsorption, then dry at 60 DEG C, and measure water content in good time, water content is made to control between 3-5%, be prepared into the microbial inoculum containing subtilis and bacillus polymyxa living spores respectively, by saccharomycetic the fermented liquid peat composed of rotten mosses of 3 times of fermented liquid weight or other carriers, uniform adsorption, then dries, and measures water content in good time, make water content control between 8-10% at 40 DEG C, is prepared into the microbial inoculum containing spore of living, then get above-mentioned three kinds of microbial inoculums mixing of equivalent weight respectively, make containing subtilis, bacillus polymyxa and saccharomycetic mixed biologic preparation.
2. the compound organic microbial fertilizer prepared of manufacture method according to claim 1.
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| CN104230586A (en) * | 2014-08-22 | 2014-12-24 | 合肥四方磷复肥有限责任公司 | Strawberry compound organic fertilizer for accelerating growth cycle and preparation method thereof |
| CN104193536B (en) * | 2014-09-22 | 2016-03-23 | 广东省农业科学院农业资源与环境研究所 | A kind of charcoal base stability Chemical Mixed Fertilizer and preparation method thereof |
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| CN107371527B (en) * | 2017-08-08 | 2020-06-09 | 江苏丘陵地区镇江农业科学研究所 | Ecological prevention and control method for strawberry continuous cropping diseases |
| CN108101714A (en) * | 2018-02-11 | 2018-06-01 | 中国科学院东北地理与农业生态研究所 | A kind of millet special bio charcoal base organic fertilizer and preparation method thereof |
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| CN110437017A (en) * | 2019-09-10 | 2019-11-12 | 中国科学院生态环境研究中心 | A kind of method that feces of livestock and poultry produces microbial manure and controls agricultural non -point pollution |
| CN112341275A (en) * | 2020-11-11 | 2021-02-09 | 宁夏陈敷生态科技有限公司 | Good carrier capable of preserving agricultural microbial agent for long time |
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| US6878179B2 (en) * | 2001-12-31 | 2005-04-12 | Microbes, Inc. | Fertilizer compositions and methods of making and using same |
| CN101429059A (en) * | 2008-12-11 | 2009-05-13 | 张庆国 | Green organic agronomic crop microorganism bacterial manure and method of producing the same |
| CN101643718B (en) * | 2009-08-26 | 2011-04-13 | 宋彦耕 | Microbial strain agent and organic fertilizers fermented by same |
| CN101691549B (en) * | 2009-09-28 | 2011-02-02 | 南京农业大学 | Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof |
| CN102876604A (en) * | 2012-09-23 | 2013-01-16 | 江苏丘陵地区镇江农业科学研究所 | Bacillus subtilis DJ-6 and application thereof to prevention and treatment of strawberry disease |
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Effective date of registration: 20160408 Address after: 212400, Huayang village, Huayang Town, Jurong, Jiangsu, Zhenjiang Patentee after: Jurong Yada Biotechnology Co. Ltd. Address before: 212400 No. 112, Zhenning Hangzhou Road, Huayang, Jiangsu, Jurong Patentee before: Jiangsu Lvdun Plant Protection Pesticide Experiment Co., Ltd. |