CN106938942A - Biological organic fertilizer with prevention and control pakchoi anthracnose function and preparation method thereof - Google Patents

Biological organic fertilizer with prevention and control pakchoi anthracnose function and preparation method thereof Download PDF

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Publication number
CN106938942A
CN106938942A CN201710037686.5A CN201710037686A CN106938942A CN 106938942 A CN106938942 A CN 106938942A CN 201710037686 A CN201710037686 A CN 201710037686A CN 106938942 A CN106938942 A CN 106938942A
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pakchoi
organic fertilizer
biological organic
anthracnose
prevention
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徐永平
张楠
李晓宇
王丽丽
陈岩
王佳宁
周通
贾藏藏
曲芳京
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Dalian University of Technology
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Dalian University of Technology
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Soil Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides a kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, raw material composition includes:Paenibacillus polymyxa, bacillus subtilis, animal wastes and plant residue are windrow and poly- γ glutamic acid solutions prepared by raw material.Present invention also offers the preparation method of above-mentioned biological organic fertilizer, the mixed bacteria liquid being made after the two kinds of Antagonistic Fungi culture activation of Paenibacillus polymyxa and bacillus subtilis is inoculated in the windrow prepared using animal wastes and plant residue as raw material, after the product that becomes thoroughly decomposed that fermentation is obtained, add poly- γ glutamic acid solutions, dry in the shade, the biological organic fertilizer with prevention and control pakchoi anthracnose function is made.Biological organic fertilizer produced by the present invention has significant control effect to pakchoi anthracnose;The simple production process of product of the present invention, extensively, production cost is low for raw material sources, is adapted to biological organic fertilizer of the large-scale production with prevention and control pakchoi anthracnose function.

Description

Biological organic fertilizer with prevention and control pakchoi anthracnose function and preparation method thereof
Technical field
The present invention relates to biological organic fertilizer field, there is prevention and control pakchoi anthracnose function more specifically to one kind Biological organic fertilizer and preparation method thereof.
Background technology
Pakchoi (Bok choy) is that herbaceous plant of the Cruciferae Brassica genus using leaf as product is worldwide important vegetable Dish crop, originates in Mediterranean and China, maximum with Chinese cultivated area, total output highest.Pakchoi anthracnose is by wishing This golden anthrax-bacilus (Colletotrichum higginsanum Sacc.) infect caused worldwide disease, and this disease is in gas Warm relatively low, humidity larger early spring and late fall morbidity are more serious, and having a humid climate, the area that cools is easier to prevalence.Pakchoi anthrax Disease is one of pakchoi Major Diseases, can cause the pakchoi significantly underproduction, and serious field almost has no harvest.
It is scale of being dissolved without enough farmlands, intensive simultaneously as China's aquaculture is separated with planting industry Substantial amounts of Stock raising waste produced by aquaculture, causes to have a strong impact on to ecological environment.And the chemical fertilizer amount of application in farmland Cumulative year after year, is increasingly highlighted to the contribution bottleneck of crop yield.
It is for the main prevention and controls of pakchoi anthracnose at present:Disease-resistant variety and chemical control, it is just for indivedual Kind effectively, and can destroy the ecological balance using chemical agent for a long time, cause rampant Minor diseases, medicament residual, environment dirty The problems such as dye.Therefore, with reference to disease-resistant variety seed selection and possess the preventing control method such as biological prevention and control of beneficial bacterium of antagonism function will It is the prevention and control trend of following soilborne fungal pathogens.
The content of the invention
It is an object of the invention to prepare a kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, using to small The antagonistic Bacillus that the pathogen of Chinese cabbage anthracnose has obvious inhibiting effect prepares biological organic fertilizer, can increase soil fertility, Prevention and control plant disease, reduces the applications of pesticide.
To reach above-mentioned purpose, the invention provides a kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, The biological organic fertilizer includes:Paenibacillus polymyxa, bacillus subtilis, animal wastes and plant residue are prepared for raw material Windrow and poly- γ glutamic acid solutions;
Wherein, described biological organic fertilizer is prepared as follows:By Paenibacillus polymyxa and bacillus subtilis two Plant the mixed bacteria liquid being made after Antagonistic Fungi culture activation and be inoculated in the windrow prepared using animal wastes and plant residue as raw material In, after the obtained product that becomes thoroughly decomposed that ferments, poly- γ glutamic acid solutions are added, are dried in the shade, being made has prevention and control pakchoi anthracnose function Biological organic fertilizer.
Under preferred embodiment, the mixed bacteria liquid is that Paenibacillus polymyxa bacterium solution is mixed with bacillus subtilis bacterium solution equal proportion Close;The cell concentration of the Paenibacillus polymyxa bacterium solution and bacillus subtilis bacterium solution is 108~109CFU·ml-1
The mass ratio of the animal wastes and plant residue is 3.5~4.5:1~1.5;
In terms of the ratio of the Antagonistic Fungi mixed bacteria liquid volume and the windrow quality, the Antagonistic Fungi mixed bacteria liquid connects The ratio of kind is 1~5ml bacterium solutions/100g windrows;
The poly- γ glutamic acid solutions added are 3.0~5.0 with the volume mass ratio of the composite fermentation product:100 (ml/g)。
Under preferred embodiment, the Paenibacillus polymyxa is Paenibacillus polymyxa (Paenibacillus polymyxa) ABTNL-X1;Bacillus subtilis is stated for bacillus subtilis (Bacillus subtilis) ABTNL-2+CGMCC+ NO.10038。
Living bacteria count >=5 × 10 in biological organic fertilizer made from the inventive method8CFUg-1, the content of organic matter >=45%.
Above-mentioned bacillus subtilis ABTNL-2 deposit numbers:CGMCC NO.10038, Paenibacillus polymyxa ABTNL-X1 It can be obtained from Chinese agriculture Microbiological Culture Collection administrative center, can also be by voluntarily isolated, except special bacterial strain situation Outside, both universal microbial inoculums are suitable for the present invention.
Present invention also offers a kind of preparation method of above-mentioned biological organic fertilizer, by Paenibacillus polymyxa and withered grass gemma The mixed bacteria liquid being made after two kinds of Antagonistic Fungi culture activation of bacillus is inoculated in using animal wastes and plant residue to be prepared as raw material Windrow in, after the obtained product that becomes thoroughly decomposed of fermenting, add poly- γ glutamic acid solutions, dry in the shade, being made has prevention and control pakchoi anthrax The biological organic fertilizer of sick function.
Further optimization, specific steps include:
S1, compost foundation:Mixed after animal wastes and plant residue are crushed, the animal wastes and plant residue Mass ratio is 3.5~4.5:1~1.5, control moisture content obtains windrow 50~65% in mixed windrow;
S2, prepare Antagonistic Fungi mixed bacteria liquid and poly- γ glutamic acid solutions:By two kinds of Antagonistic Fungi Paenibacillus polymyxas, withered grass After bacillus culture activation, obtain cell concentration and be 108~109CFU·ml-1Bacterium solution, two kinds of bacterium solution equal proportions are mixed Close, Antagonistic Fungi mixed bacteria liquid is made;
S3, inoculation Antagonistic Fungi mixed bacteria liquid:Antagonistic Fungi mixed bacteria liquid made from step S2 is inoculated into made from step S1 In windrow, progress, which is fermented, obtains composite fermentation product;
In terms of the ratio of the Antagonistic Fungi mixed bacteria liquid volume and the windrow quality, the Antagonistic Fungi mixed bacteria liquid connects The ratio of kind is 1~5ml bacterium solutions/100g windrows;
S4, prepare biological organic fertilizer:It is 200mgL that concentration is added into composite fermentation product made from step S3-1It is poly- γ glutamic acid solutions, are mixed, and it is 15~30% to dry in the shade to total moisture content, that is, obtains the life with prevention and control pakchoi anthracnose function Organic fertilizer;
The poly- γ glutamic acid solutions added are 3.0~5.0 with the volume mass ratio of the composite fermentation product:100 (ml/g)。
Animal wastes described in step S1 are pig manure, chicken manure or cow dung;The plant residue is withered grass, branch or the straw crushed Stalk.
Under preferred embodiment, Paenibacillus polymyxa described in step S2 is Paenibacillus polymyxa (Paenibacillus polymyxa)ABTNL-X1;Bacillus subtilis is stated for bacillus subtilis (Bacillus subtilis) ABTNL-2+ CGMCC+NO.10038。
Under preferred embodiment, the Liquid Culture basigamy that Antagonistic Fungi culture described in step S2 is activated is:Every liter of distilled water adds chlorine Change sodium 10.0g, tryptone 10.0g, dusty yeast 5.0g, through 121 DEG C, sterilize within 20 minutes.
It is further preferred that the condition fermented described in step S3 is:Material is placed in layering in compost container, and is uniformly sprinkled Spill and be sufficiently mixed after liquid bacterium solution progress microorganism aerobic fermentation, temperature rises therewith, reaches and observation is noted after 55~65 DEG C, when Turning is carried out when windrow temperature is decreased obviously first, and is kept the skin wet, windrow temperature is raised again, and temperature is obvious again During decline, turning again, and so on 2~3 times, until windrow temperature obtains composite fermentation product when being higher than within 5 DEG C of room temperature.
10 DEG C or so of temperature drop of the present invention is thought to be decreased obviously;The specific standards that the present invention keeps the skin wet are in compost Initial water is divided into 60%, and it is not fixed ratio subsequently to keep the skin wet, and can maintain fermentation.
Compost free from extraneous odour produced by the present invention, do not attract mosquito, percentage of seedgermination>80%th, worm's ovum content 0% meets country Standard.
The present invention technological innovation be:
1st, the present invention screens two obtained bacillus Paenibacillus polymyxa ABTNL-X1, withered grass from animal wastes Bacillus ABTNL-2 thalline can have the antagonism of competition nutrition to John Higgins anthrax-bacilus in itself, and can produce withered grass Rhzomorph, polymyxins, isoreactivity metabolite, it has obvious inhibitory action to various pathogens such as John Higgins anthrax-bacilus.
2nd, biological organic fertilizer prepared by the present invention can increase soil fertility, and be conducive to Zinc fractions crumb structure to improve Soil property, promotes plant seed germination and growth, strengthens plant stress-resistance ability, improves product quality, product is reached green Or organic standard.On this basis, moreover it is possible to prevention and control plant disease, the reduction applications of pesticide to a certain extent, play and achieve many things at one stroke Function.
3rd, the biological organic fertilizer for preparing of the present invention can improved soil, improve crop quality and can be by strengthening crop disease-resistant Property, suppress using microbial diversity the prevention and control such as pathogen and control soilborne fungal pathogens, and can provide nutrition for Antagonistic Fungi, Suppression by Antagonistic Fungi itself and its metabolite to pathogen reaches good prevention effect.
Preservation explanation
The preservation information of biological material specimens of the present invention:The microorganism (strain) for joining evidence is ABTNL-2, Classification And Nomenclature For bacillus subtilis (Bacillus subtilis), managed and entrusted by Chinese microorganism strain preservation on November 21st, 2014 Member can common micro-organisms center (abbreviation CGMCC) preservation, deposit number CGMCC NO.10038.CGMCC addresses are court of Beijing The positive institute 3 of area's North Star West Road 1.
Embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with Any mode limits the present invention.
The present invention provides following examples and comparative, illustrates the present invention to pakchoi anthrax by taking pakchoi anthracnose as an example The specific effect of the control effect of disease.
Embodiment 1
A kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, is made by the production method of following steps:
1st, the preparation of windrow
After the plant residue such as the animal wastes such as pig manure, chicken manure or cow dung and withered grass, branch or stalk is crushed in mass ratio 3.5:After 1 mixing, adjustment windrow moisture content is 60%, is saved backup.
2nd, the preparation of mixed bacteria liquid and poly- γ glutamic acid solutions:
Cultivate Antagonistic Fungi Paenibacillus polymyxa ABTNL-X1, bacillus subtilis ABTNL respectively with fluid nutrient medium-2, Equal proportion is mixed, and mixed bacteria liquid is made;Preparation concentration is 200mgL-1Poly- γ glutamic acid solutions.
Wherein Liquid Culture basigamy is that sodium chloride 10.0g, tryptone 10.0g, dusty yeast 5.0g are dissolved in 1L distilled water In, through 121 DEG C, used after sterilizing in 20 minutes;Cell concentration is 10 in every kind of antagonism bacterium solution9CFU·mL-1
3rd, the foundation of compost and condition are controlled
By the mixed bacteria liquid prepared in step 2 with bacterium solution volume and windrow mass ratio (hereinafter referred to as volume mass ratio) 1.0% It is added in the windrow prepared in step 1.Layering placed and fully mixed after material, and liquid bacterium solution of uniformly splashing in compost container Close and carry out microorganism aerobic fermentation, temperature rises therewith, reaches and maintain 55~65 DEG C of high temperature;When windrow temperature occurs first Turning is carried out when being decreased obviously, and is kept the skin wet, windrow temperature is raised again, equally, when temperature is decreased obviously, and is turned over again Heap, and so on 2~3 times until windrow temperature close to or a little higher than room temperature, and compost free from extraneous odour, do not attract mosquito.Determine Its percentage of seedgermination>80%th, when worm's ovum content is 0%, compost maturity product is saved backup.
4th, the preparation of the biological organic fertilizer with prevention and control pakchoi anthracnose function
Fully mixed than the 3.0% poly- γ glutamic acid solutions of addition with volume mass into step 3 gained compost maturity product After dry in the shade to water content be 15~30%, living bacteria count>5×108CFU·g-1, the content of organic matter>45%, meet national mark It is accurate.By product grinding and sieving, powder is made, can need to be granulated according to business, being made has prevention and control pakchoi anthracnose The biological organic fertilizer of function.
Embodiment 2
A kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, is made by the production method of following steps:
1st, the preparation of windrow
After the plant residue such as the animal wastes such as pig manure, chicken manure or cow dung and withered grass, branch or stalk is crushed in mass ratio 3.5:After 1 mixing, adjustment windrow moisture content is 60%, is saved backup.
2nd, the preparation of mixed bacteria liquid and poly- γ glutamic acid solutions:
Cultivate Antagonistic Fungi Paenibacillus polymyxa ABTNL-X1, bacillus subtilis ABTNL-2 respectively with fluid nutrient medium, Equal proportion is mixed, and mixed bacteria liquid is made;Preparation concentration is 200mgL-1Poly- γ glutamic acid solutions.
Wherein Liquid Culture basigamy is that sodium chloride 10.0g, tryptone 10.0g, dusty yeast 5.0g are dissolved in 1L distilled water In, through 121 DEG C, used after sterilizing in 20 minutes;Cell concentration is 10 in every kind of antagonism bacterium solution8~109CFU·mL-1
3rd, the foundation of compost and condition are controlled
The mixed bacteria liquid prepared in step 2 is added with bacterium solution volume with windrow mass ratio (hereinafter referred to as volume mass ratio) 5% It is added in the windrow prepared in step 1.Layering is sufficiently mixed after placing material, and liquid bacterium solution of uniformly splashing in compost container Microorganism aerobic fermentation is carried out, temperature rises, reaches and maintain 55 DEG C of high temperature above therewith;When windrow temperature occurs substantially first Turning is carried out during decline, and is kept the skin wet, windrow temperature is raised again, equally, when temperature is decreased obviously, again turning, such as This reciprocal 2~3 times until windrow temperature close to or a little higher than room temperature, and compost free from extraneous odour, do not attract mosquito.Determine its seed Germination percentage>80%th, when worm's ovum content is 0%, compost maturity product is saved backup.
4th, the preparation of the biological organic fertilizer with prevention and control pakchoi anthracnose function
Fully mixed than the 3.0% poly- γ glutamic acid solutions of addition with volume mass into step 3 gained compost maturity product After dry in the shade to water content be 15~30%, living bacteria count>5×108CFU·g-1, the content of organic matter>45%, meet national mark It is accurate.By product grinding and sieving, powder is made, can need to be granulated according to business, being made has prevention and control pakchoi anthracnose The biological organic fertilizer of function.
Comparative example 1 (embodiment 2 lacks bacillus subtilis ABTNL-2)
A kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, is made by the production method of following steps:
1st, the preparation of windrow
After the plant residue such as the animal wastes such as pig manure, chicken manure or cow dung and withered grass, branch or stalk is crushed in mass ratio 3.5:After 1 mixing, adjustment windrow moisture content is 60%, is saved backup.
2nd, bacterium solution and the preparation of poly- γ glutamic acid solutions:
After fluid nutrient medium culture Antagonistic Fungi Paenibacillus polymyxa ABTNL-X1, bacterium solution is made;Preparing concentration is 200mg·L-1Poly- γ glutamic acid solutions.
Wherein Liquid Culture basigamy is that sodium chloride 10.0g, tryptone 10.0g, dusty yeast 5.0g are dissolved in 1L distilled water In, through 121 DEG C, used after sterilizing in 20 minutes;Cell concentration is 10 in antagonism bacterium solution8~109CFU·mL-1
3rd, the foundation of compost and condition are controlled
The bacterium solution prepared in step 2 is added to bacterium solution volume with windrow mass ratio (hereinafter referred to as volume mass ratio) 5% In the windrow prepared in step 1.Layering is sufficiently mixed progress after placing material, and liquid bacterium solution of uniformly splashing in compost container Microorganism aerobic ferments, and temperature rises therewith, reaches and maintain more than 55 DEG C of high temperature;Under windrow temperature occurs substantially first Turning is carried out during drop, and is kept the skin wet, windrow temperature is raised again, equally, when temperature is decreased obviously, again turning, so Reciprocal 2~3 times until windrow temperature close to or a little higher than room temperature, and compost free from extraneous odour, do not attract mosquito.Determine its seed hair Bud rate>80%th, when worm's ovum content is 0%, compost maturity product is saved backup.
4th, the preparation of the biological organic fertilizer with prevention and control pakchoi anthracnose function
Fully mixed than the 3.0% poly- γ glutamic acid solutions of addition with volume mass into step 3 gained compost maturity product After to dry in the shade to water content be 15~30%, and living bacteria count>5×108CFU·g-1, the content of organic matter>45%, meet country Standard.By product grinding and sieving, powder is made, can need to be granulated according to business, being made has prevention and control pakchoi anthrax The biological organic fertilizer of sick function.
Comparative example 2 (embodiment 2 lacks Paenibacillus polymyxa ABTNL-X1)
A kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, is made by the production method of following steps:
1st, the preparation of windrow
After the plant residue such as the animal wastes such as pig manure, chicken manure or cow dung and withered grass, branch or stalk is crushed in mass ratio 3.5:After 1 mixing, adjustment windrow moisture content is 60%, is saved backup.
2nd, bacterium solution and the preparation of poly- γ glutamic acid solutions:
After fluid nutrient medium culture Antagonistic Fungi bacillus subtilis ABTNL-2, bacterium solution is made;Preparing concentration is 200mg·L-1Poly- γ glutamic acid solutions.
Wherein Liquid Culture basigamy is that sodium chloride 10.0g, tryptone 10.0g, dusty yeast 5.0g are dissolved in 1L distilled water In, through 121 DEG C, used after sterilizing in 20 minutes;Cell concentration is 10 in antagonism bacterium solution8~109CFU·mL-1
3rd, the foundation of compost and condition are controlled
The bacterium solution prepared in step 2 is added to bacterium solution volume with windrow mass ratio (hereinafter referred to as volume mass ratio) 5% In the windrow prepared in step 1.Layering is sufficiently mixed progress after placing material, and liquid bacterium solution of uniformly splashing in compost container Microorganism aerobic ferments, and temperature rises therewith, reaches and maintain more than 55 DEG C of high temperature;Under windrow temperature occurs substantially first Turning is carried out during drop, and is kept the skin wet, windrow temperature is raised again, equally, when temperature is decreased obviously, again turning, so Reciprocal 2~3 times until windrow temperature close to or a little higher than room temperature, and compost free from extraneous odour, do not attract mosquito.Determine its seed hair Bud rate>80%th, when worm's ovum content is 0%, compost maturity product is saved backup.
4th, the preparation of the biological organic fertilizer with prevention and control pakchoi anthracnose function
Fully mixed than the 3.0% poly- γ glutamic acid solutions of addition with volume mass into step 3 gained compost maturity product After to dry in the shade to water content be 15~30%, and living bacteria count>5×108CFU·g-1, the content of organic matter>45%, meet country Standard.By product grinding and sieving, powder is made, can need to be granulated according to business, being made has prevention and control pakchoi anthrax The biological organic fertilizer of sick function.
Effect of fertilizer is tested:
Totally 5 groups carry out the planting experiment of pakchoi, every group of 100 plants of crops by experiment point A, B, C, D and E, and set three Individual repetition.A groups apply biological organic fertilizer prepared by embodiment 1;B groups apply biological organic fertilizer prepared by embodiment 2;C and D components Not Shi Yong comparative example 3 and 4 prepare biological organic fertilizer;E groups are control group, and soil does not apply fertilizer and (applies equivalent soil).
The preparation of soil and seed:
The 121 DEG C of sterilizing 20min of fine and closely woven soil screened through 8 mesh stainless steel mesh are wherein influenceed into seed growth to remove Saved backup after various microorganisms.
300 complete, pakchoi seed of full seed, shape is chosen, is sterilized with liquor potassic permanganate (0.05%) Saved backup after being rinsed well repeatedly with water after 30min, and 24h is facilitated under the conditions of 37 DEG C.
Planting experiment:Added in planting groove and be uniformly blended into the soil of biological organic fertilizer (biological organic fertilizer is with 10thm-2 Dosage administration) sow afterwards, and bury seed with a certain amount of soil.Cultivated after the completion of operating above in constant incubator, Daily to carry out conventional management of watering, at 15~20 days, crop is in Seedling Stage.Foot is inoculated with to the planting soil of pakchoi seedling Pakchoi pathogen-John Higgins anthrax-bacilus of amount.It is inoculated with after germ, basic basic management is constant in 5~10 days, observed and recorded Ill strain number simultaneously calculates ratio.
The disease state of each group plant is counted,
Biological organic fertilizer prepared by the embodiment of table 1 and comparative example passes the control effect of plant disease to soil
Group Incidence of disease %
The biological organic fertilizer of A embodiments 1 3
The biological organic fertilizer of B embodiment 2 2
The biological organic fertilizer of C comparative examples 1 53
The biological organic fertilizer of D comparative examples 2 55
E control groups soil generation fertilizer 91
From the result in table 1, by applying biological organic fertilizer A groups and B group phases prepared by embodiment 1 and embodiment 2 Compared with the control group not applied fertilizer, pakchoi illness strain number is substantially on the low side, and prevention and control rate reaches more than 85%, and lacks any of which one During kind of Antagonistic Fungi, its control effect to soilborne fungal pathogens is substantially reduced.This shows biological organic fertilizer pair prepared by the present invention Pakchoi anthrax disease has effective control effect, and this is due to wherein there are a large amount of distinctive Paenibacillus polymyxas and withered The Inhibiting and protective function of careless bacillus and its metabolite to John Higgins anthrax-bacilus.The mixed bacteria liquid content of embodiment 2 is high, The incidence of disease is reduced, and more preferably, concentration is presented related in biological organic fertilizer prevention effect.In summary, the biological organic that prepared by the present invention The administration of fertilizer has significant control effect to pakchoi anthracnose disease, can be applied in agricultural production practice.
The foregoing is intended to be a preferred embodiment of the present invention, but protection scope of the present invention is not limited thereto, Any one skilled in the art in the technical scope of present disclosure, technique according to the invention scheme and its Inventive concept is subject to equivalent or change, should all be included within the scope of the present invention.

Claims (9)

1. a kind of biological organic fertilizer with prevention and control pakchoi anthracnose function, it is characterised in that raw material composition includes:More viscous class Bacillus, bacillus subtilis, animal wastes and plant residue are windrow and poly- γ glutamic acid solutions prepared by raw material;
Wherein, the raw material is handled by the following method:By the two kinds of Antagonistic Fungi cultures of Paenibacillus polymyxa and bacillus subtilis The mixed bacteria liquid being made after activation is inoculated in the windrow prepared using animal wastes and plant residue as raw material, what fermentation was obtained Become thoroughly decomposed after product, add poly- γ glutamic acid solutions, dry in the shade, the biological organic fertilizer with prevention and control pakchoi anthracnose function is made.
2. there is the biological organic fertilizer of prevention and control pakchoi anthracnose function according to claim 1, it is characterised in that described mixed Bacterium solution is closed to mix with bacillus subtilis bacterium solution equal proportion for Paenibacillus polymyxa bacterium solution;The Paenibacillus polymyxa bacterium solution 10 are with the cell concentration of bacillus subtilis bacterium solution8~109CFU·ml-1
The mass ratio of the animal wastes and plant residue is 3.5~4.5:1~1.5;
In terms of the ratio of the Antagonistic Fungi mixed bacteria liquid volume and the windrow quality, the inoculation ratio of the Antagonistic Fungi mixed bacteria liquid Example is 1~5ml bacterium solutions/100g windrows;
The poly- γ glutamic acid solutions added are 3.0~5.0 with the volume mass ratio of the composite fermentation product:100(ml/ g)。
3. the biological organic fertilizer with prevention and control pakchoi anthracnose function is stated according to claim 2, it is characterised in that described to glue more Series bacillus is Paenibacillus polymyxa (Paenibacillus polymyxa) ABTNL-X1;
Bacillus subtilis is stated for bacillus subtilis (Bacillus subtilis) ABTNL-2+CGMCC+NO.10038.
4. the preparation method of the biological organic fertilizer with prevention and control pakchoi anthracnose function described in claim 1, it is characterised in that The mixed bacteria liquid being made after the two kinds of Antagonistic Fungi culture activation of Paenibacillus polymyxa and bacillus subtilis is inoculated in animal In the windrow that excrement is prepared with plant residue for raw material, after the obtained product that becomes thoroughly decomposed that ferments, poly- γ glutamic acid solutions are added, it is cloudy It is dry, the biological organic fertilizer with prevention and control pakchoi anthracnose function is made.
5. there is the preparation method of the biological organic fertilizer of prevention and control pakchoi anthracnose function, its feature according to claim 4 It is that specific steps include:
S1, compost foundation:Mixed after animal wastes are crushed with plant residue, the quality of the animal wastes and plant residue Than for 3.5~4.5:1~1.5, control moisture content obtains windrow 50~65% in mixed windrow;
S2, prepare Antagonistic Fungi mixed bacteria liquid and poly- γ glutamic acid solutions:By two kinds of Antagonistic Fungi Paenibacillus polymyxas, withered grass gemma After bacillus culture activation, obtain cell concentration and be 108~109CFU·ml-1Bacterium solution, two kinds of bacterium solution equal proportions are mixed, system Obtain Antagonistic Fungi mixed bacteria liquid;
S3, inoculation Antagonistic Fungi mixed bacteria liquid:Antagonistic Fungi mixed bacteria liquid made from step S2 is inoculated into windrow made from step S1 In, progress, which is fermented, obtains composite fermentation product;
In terms of the ratio of the Antagonistic Fungi mixed bacteria liquid volume and the windrow quality, the inoculation ratio of the Antagonistic Fungi mixed bacteria liquid Example is 1~5ml bacterium solutions/100g windrows;
S4, prepare biological organic fertilizer:It is 200mgL that concentration is added into composite fermentation product made from step S3-1Poly- γ paddy Propylhomoserin solution, is mixed, and it is 15~30% to dry in the shade to total moisture content, that is, obtaining the biology with prevention and control pakchoi anthracnose function has Machine fertilizer;
The poly- γ glutamic acid solutions added are 3.0~5.0 with the volume mass ratio of the composite fermentation product:100(ml/ g)。
6. there is the preparation method of the biological organic fertilizer of prevention and control pakchoi anthracnose function, its feature according to claim 5 It is that animal wastes described in step S1 are pig manure, chicken manure or cow dung;The plant residue is withered grass, branch or the stalk crushed.
7. there is the preparation method of the biological organic fertilizer of prevention and control pakchoi anthracnose function, its feature according to claim 5 It is that Paenibacillus polymyxa described in step S2 is Paenibacillus polymyxa (Paenibacillus polymyxa) ABTNL- X1;
Bacillus subtilis is stated for bacillus subtilis (Bacillus subtilis) ABTNL-2+CGMCC+NO.10038.
8. there is the preparation method of the biological organic fertilizer of prevention and control pakchoi anthracnose function, its feature according to claim 5 It is that the Liquid Culture basigamy that Antagonistic Fungi culture described in step S2 is activated is:Every liter of distilled water adds sodium chloride 10.0g, pancreas egg White peptone 10.0g, dusty yeast 5.0g, through 121 DEG C, sterilize for 20 minutes.
9. there is the preparation method of the biological organic fertilizer of prevention and control pakchoi anthracnose function, its feature according to claim 5 It is that the condition fermented described in step S3 is:Layering is placed after material, and liquid bacterium solution of uniformly splashing fully in compost container Mixing carries out microorganism aerobic fermentation, and temperature rises therewith, reaches and observation is noted after 55~65 DEG C, when windrow temperature occurs first Turning is carried out when being decreased obviously, and is kept the skin wet, windrow temperature is raised again, when temperature is decreased obviously again, again turning, And so on 2~3 times, until windrow temperature obtains composite fermentation product when being higher than within 5 DEG C of room temperature.
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CN107668073A (en) * 2017-10-10 2018-02-09 云南农业大学 A kind of bacillus polymyxa biological agent and preparation method for preventing and treating plant root rot
CN108184554A (en) * 2018-01-29 2018-06-22 广东碧然美景观艺术有限公司 A kind of turf weed preventing control method
CN110437017A (en) * 2019-09-10 2019-11-12 中国科学院生态环境研究中心 A kind of method that feces of livestock and poultry produces microbial manure and controls agricultural non -point pollution
CN114262248A (en) * 2022-01-13 2022-04-01 宁波市农业科学研究院 Biological bacterial fertilizer for preventing and treating soft rot of pakchoi and preparation method thereof

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CN105218213A (en) * 2015-08-18 2016-01-06 大连理工大学 There is biological organic fertilizer of prevention and control soilborne fungal pathogens function and preparation method thereof

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CN101691549A (en) * 2009-09-28 2010-04-07 南京农业大学 Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof
CN104496580A (en) * 2014-11-30 2015-04-08 杨秋慧 Straw decomposition agent prepared from methane residues
CN105218213A (en) * 2015-08-18 2016-01-06 大连理工大学 There is biological organic fertilizer of prevention and control soilborne fungal pathogens function and preparation method thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107668073A (en) * 2017-10-10 2018-02-09 云南农业大学 A kind of bacillus polymyxa biological agent and preparation method for preventing and treating plant root rot
CN108184554A (en) * 2018-01-29 2018-06-22 广东碧然美景观艺术有限公司 A kind of turf weed preventing control method
CN110437017A (en) * 2019-09-10 2019-11-12 中国科学院生态环境研究中心 A kind of method that feces of livestock and poultry produces microbial manure and controls agricultural non -point pollution
CN114262248A (en) * 2022-01-13 2022-04-01 宁波市农业科学研究院 Biological bacterial fertilizer for preventing and treating soft rot of pakchoi and preparation method thereof

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