CN109306328A - Saline-alkali tolerant bacterial manure microorganism be separately cultured and serial bacterial fertilizer products production technology - Google Patents
Saline-alkali tolerant bacterial manure microorganism be separately cultured and serial bacterial fertilizer products production technology Download PDFInfo
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- CN109306328A CN109306328A CN201710649490.1A CN201710649490A CN109306328A CN 109306328 A CN109306328 A CN 109306328A CN 201710649490 A CN201710649490 A CN 201710649490A CN 109306328 A CN109306328 A CN 109306328A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses the preparation methods of a kind of saline-alkali tolerant bacterial manure or saline-alkali tolerant bacterium product, it is characterized by: by saline-alkali tolerant growth promotion composite bacteria agent A and saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B with 1: (0.1-10) ratio mixes, take the microbial inoculum after mixing, the residual culture medium of agriculture with high salt is added and obtains bacterial manure or bacterium product, the composite bacteria agent includes: rihizobium japonicum, bacillus megaterium, bacillus cereus, bacillus subtilis, colloid bacillus cereus, bacillus licheniformis, Bu Shi Bacillus acidi lactici, bacillus amyloliquefaciens, bacillus pumilus, mostly viscous class gemma and Trichoderma harzianum composition.The bacterial manure or bacterium product is promoting plant growth or is improving the application in crop yield or planting soil improvement and in raising crop disease-resistant ability.The product forms solid bacterial manure, drying or gel starch bacterial manure, the bacterial manure of liquid form, agriculture maimed person material bacterial manure, turf bacterial manure, peat bacterial manure, suspend mode bacterial manure, pig, cattle and sheep chicken manure bacterial manure and forms kind of a clothing in conjunction with different bacterial manure carriers.
Description
Technical field
The present invention relates to the preparation method of a kind of saline-alkali tolerant bacterial manure or saline-alkali tolerant bacterium product, belong to microbe technology with
Production of manure technology.
Background technique
Bacterial manure is also known as bio-fertilizer, bio-feritlizer, bacterial fertilizer or Inoculant etc., containing a large amount of microorganism, in the soil
By the vital movement of microorganism, improve the nutritional condition of crop, participates in turn of the fixation of nitrogen in soil, matter and energy
Change, the formation of humus and decomposition, the release of nutrient.It is that artificial mode increases quantity of useful microbe into soil, it will be able to
The activity for enhancing Soil Microorganism, to improve the fertility of soil.
Bio-hormone stimulates plant growth.No matter microorganism in more spore bacterial manure in its fermentation process (can be regarded as micro- life
The self-reproduction process of object), or during vital movement in soil, can generate a large amount of using effects gibberellin and
The substances such as cytohormones, these substances can adjust the growth of the metabolism stimulation crop of crop after root system of plant contact,
To make crop generate effect of increasing production, inhibit and reduce the invasion and breeding chance, stimulation organic matter release nutrition of cause of disease
Microorganism.Furthermore it is possible to be designed by function bacterium, being formed improves environment, the bacterial manure of farmland pollution ecological management.
No.1 Central File in 2016 is emphasized " to establish the idea for developing that green agriculture is exactly protecting ecology, accelerate agriculture
The improvement of industry environment outstanding problem ".Currently, China is the country that fertilizer amount is most in the world, year fertilizer amount is more than 4.2 ×
1010kg accounts for the world 30% or so, and nitrogenous fertilizer this season utilization rate is averagely only 30% or so, and the 70% of phosphate fertilizer is consolidated by adsorption by soil
Surely the phosphate for being converted to slightly solubility is accumulated by soil, shows national 13 grain main products according to Ministry of Agriculture's survey data in 2004
It saves in 416,070,000 mu of arable lands, surpasses warning area up to 504.5 ten thousand mu, account for the 18.49% of the monitoring gross area；Exceeded area is polluted to reach
227.5 ten thousand mu, account for the 8.34% of the monitoring gross area.In addition, national sewage irrigation area is up to more than 5,400 ten thousand mu, every year because of heavy metal
10,000,000,000 kilograms of underproduction grain of pollution, pollutes 12,000,000,000 kilograms of exceeded grain, and adding up to economic loss is more than 20,000,000,000 yuan；More than 4000 ten thousand
People lives in irrigating region for a long time.The Farmland contamination of large area leads to effective Cultivated Land Area Decrease, and pollution of agricultural products is exceeded, agricultural
Integrated production capacity decline, region disease popularity, human health decline, agricultural and rural economy loss are huge.According to the preliminary statistics,
National annual 180~2,000,000 people of pathogenesis of cancer number, dead 140~1,500,000 people are exceeded with pollution of agricultural products to a certain extent
It is related.
It include that (chemical fertilizer and chemical pesticide are dirty for chemical contamination beyond the soil pollution that soil can undertake load
Dye), heavy metal pollution, field irrigation pollution, noxious waste pollution (white pollutions such as stalk, feces of livestock and poultry, mulch) and agriculture
Field ecological environment destruction.Soil acidification alkalization, promotes the release and increase of some hazardous contaminants in soil.It is unreasonable to apply
After nitrogenous fertilizer, the nitrogen of loss is accumulated in plant in the form of nitrate, is reduced the quality of food and feed, is led to agricultural product
Quality declines (especially Nitrate in Vegetables).Reach stable yields in order to kill insect, fungi and other pest and disease damages simultaneously and make
Pesticide, harmfulness is very big, and organic phosphorus high-toxic pesticide accounts for very big specific gravity in the pesticide in China, contains weight there are also some
Metal, persistent pesticide can pollute surface layer and deep soil, and stability is extremely strong, and all degradation needs many decades, Ge Bienong
Medicine is lost in environment in its composition containing heavy metals, about 85% pesticides such as Hg, As, Cu, Zn, wherein about 30% is diffused into
In atmosphere, surface water 20% is brought by agricultural drain and runoff, remains 30% in soil, enters underground water 5% with lower infiltration.
These pollutions cause in recent years, and China's food safety form is very severe, and the pollution level of some areas is serious, are more than country
III level standard.From causing the heavy metal type of China's Farmland Soil Pollution to include that Cd, As, Hg, Pb, Cu and its combined pollution are outstanding
To be obvious, wherein again exceeded the most prominent with Cd, some arable lands because Cd is exceeded and it is no longer cultivated to be forced, the arable land of heavy metal pollution and
Nearly 20,000,000 sq-km of product, accounts for about the 1/5 of China total cultivated area, wherein 10,000,000 squares of Industrial " three Waste " pollution arable land
Km, sewage irrigation is up to more than 330 ten thousand sq-kms.China every year because caused by heavy metal pollution grain drop in production weighed up to 10,000,000 t
The grain of metallic pollution is up to 12,000,000 t, and economic loss resulting from is more than 20,000,000,000 yuan of RMB.These grains are supported enough
40000000 or so population, and this pollution problem is got worse.In addition, there are also the pollution of the solid wastes such as stalk, the every annual output in China
The crop material of raw about 6.5 × 1011kg or so, has 4 one-tenth to fail to utilize, causes environmental pollution.Repair all kinds of pollutions or to it
Carrying out safe utilization is the important component in the work of China's ecological agriculture construction, is administered to farmland pollution extremely urgent.
Separating function, biocontrol water bacterial manure microorganism form functional, biocontrol water bacterial manure series of products industrialization productions
Technique is the method for the most effective nutritional condition and restoration of the ecosystem farmland pollution for improving crop.
Summary of the invention
Technical problem to be solved by the invention is to provide separation, culture and the series of a kind of saline-alkali tolerant bacterial manure microorganism
The industrialized manufacturing technique of bacterial manure microbial inoculum product.
1, the preparation method of a kind of saline-alkali tolerant bacterial manure or saline-alkali tolerant bacterium product, it is characterised in that: answer saline-alkali tolerant growth promotion
Combined bacteria agent A and saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B are with 1: (0.1-10) ratio mixes, and takes the microbial inoculum after mixing, and is added with high salt
The residual culture medium of agriculture obtains bacterial manure or bacterium product；
The residual culture medium 0.1-100g/L containing straw powder of the agriculture with high salt, NaCl 5-27g/L, meat soup (light), agar 1g/L,
Potato 10-200g/L, sucrose 1-30g/L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/L, dipotassium hydrogen phosphate 0.1-5g/L, ferment
Female powder 0.1-6g/L, PH7.5-9.5；
The active constituent of the saline-alkali tolerant growth promotion bacterium composite bacteria agent A is by rihizobium japonicum rihizobium japonicum
(Bradyrhizobium japonicum) CGMCC NO.1.2550, bacillus megaterium (Bacillus megaterium)
CGMCC NO.1.10466, bacillus cereus (Bacillus cereus) CGMCC NO. 1.10559, bacillus subtilis
(Bacillus subtilis) CGMCC NO.1.4255, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC
NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens
(Bacillus amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa)
CGMCC NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604 composition；
The active constituent of the saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B is by bacillus pumilus (Bacillus pimilus)
CGMCC NO.1.10291, bacillus licheniformis (Bacillus licheniformis) CGMCC NO. 1.884, colloid gemma
Bacillus (Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus
Buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC
NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa) CGMCC NO.1.4261 and Trichoderma harzianum
(Trichoderma harzianum) CGMCC NO.3.6604 composition.
2, according to the method described in claim 1, it is characterized by: soybean described in saline-alkali tolerant growth promotion composite bacteria agent A
Rhizobium (Bradyrhizobium japonicum) CGMCC NO.1.2550, bacillus megaterium (Bacillus
Megaterium) CGMCC NO.1.10466, bacillus cereus (Bacillus cereus) CGMCC NO.1.10559, withered
Careless bacillus (Bacillus subtilis) CGMCC NO.1.4255, colloid bacillus cereus (Bacillus
Mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus buchneri) CGMCC
NO.1.3114, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma
(Paenibacillus polymyxa) CGMCC NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC
The bacterium colony of NO.3.6604 forms living bacteria count (CFU) than being (4.3~6.5): (4.3~6.5): (4.3~6.5): (3.2~
5): (3.2~5): (3.2~5): (3.2~5).
Bacillus pumilus (Bacillus pimilus) CGMCC in the saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B
NO.1.10291, bacillus licheniformis (Bacillus licheniformis) CGMCC NO.1.884, colloid bacillus cereus
(Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus buchneri)
CGMCC NO.1.3114, it bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.1.936, glues more
Class gemma (Paenibacillus polymyxa) CGMCC NO.1.4261 and Trichoderma harzianum (Trichoderma
Harzianum) bacterium colony of CGMCC NO.3.6604 forms living bacteria count (CFU) than being (4.3~6.5): (4.3~6.5):
(4.3~6.5): (3.2~5): (3.2~5): (3.2~5): (3.2~5).
The volume ratio of microbial inoculum and the residual culture medium of agriculture with high salt after the mixing is 1-500: 1000.
3, the bacterial manure or bacterium product being prepared by method as claimed in claim 1 or 2.
4, bacterial manure as claimed in claim 3 or bacterium product are promoting plant growth or raising crop yield or planting soil to change
Application in good.
5, bacterial manure as claimed in claim 3 or bacterium product are improving the application in crop disease-resistant ability.
6, application according to claim 4 or 5, it is characterised in that: the crop is following any: rice,
Corn, beans, potato, highland barley, semen viciae fabae, wheat, oilseeds, turnip, big mustard, peanut, flax, hemp, sunflower, radish, Chinese cabbage,
Celery, leek, garlic, green onion, carrot, snake melon, lotus flower dish, jerusalem artichoke, sword bean, coriander, asparagus lettuce, chrysanthemum, capsicum, cucumber, tomato,
Caraway, pears, green plum, apple, peach, apricot, walnut, plum, cherry, strawberry, Chinese pear-leaved crabapple, jujube, sour pears, wild apricot, wild peach, mountain jujube, mountain
Cherry, sea-buckthorn, clover, corn, green manure, Chinese milk vetch, honeysuckle, ginseng, Radix Angelicae Sinensis, honeysuckle, peppermint and argy wormwood.
7, application according to claim 4 or 5, the disease are as caused by bacterium, fungi or actinomycotic infection.
Experiments have shown that the bacterial manure growing way in the application present invention is significantly better than application urea；Bacterial manure in the present invention facilitates disease-resistant.
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified
Conventional method.The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Used in the following example
Shewanella putrefaciens (Shewanella putrefacens) CGMCC NO.1.6515 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from Chinese Microbiological Culture Collection conservator
Meeting common micro-organisms center obtains the bacterial strain.
Bacillus megaterium (Bacillus megaterium) CGMCC NO.1.10466 is preserved in Chinese microorganism strain
Preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from China Committee for Culture Collection of Microorganisms
Common micro-organisms center obtains the bacterial strain.
Bacillus cereus (Bacillus cereus) CGMCC NO.1.10559 is preserved in Chinese microorganism strain preservation
Administration committee's common micro-organisms center (abbreviation CGMCC), the public can be common from China Committee for Culture Collection of Microorganisms
Microorganism center obtains the bacterial strain.
Bacillus licheniformis (Bacillus licheniformis) CGMCC NO.1.884 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from Chinese Microbiological Culture Collection conservator
Meeting common micro-organisms center obtains the bacterial strain.
Bacillus subtilis (Bacillus subtilis) CGMCC NO.1.4255 is preserved in Chinese microorganism strain guarantor
It hides administration committee's common micro-organisms center (abbreviation CGMCC), the public can be general from China Committee for Culture Collection of Microorganisms
Logical microorganism center obtains the bacterial strain.
Bacillus pumilus (Bacillus pimilus) CGMCC NO.1.10291 is preserved in Chinese microorganism strain guarantor
It hides administration committee's common micro-organisms center (abbreviation CGMCC), the public can be general from China Committee for Culture Collection of Microorganisms
Logical microorganism center obtains the bacterial strain.
It is micro- that bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.1.936 is preserved in China
Biological inoculum preservation administration committee's common micro-organisms center (abbreviation CGMCC), the public can be from Chinese Microbiological Culture Collection pipes
Reason committee common micro-organisms center obtains the bacterial strain.
Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604 is preserved in Chinese microorganism strain guarantor
It hides administration committee's common micro-organisms center (abbreviation CGMCC), the public can be general from China Committee for Culture Collection of Microorganisms
Logical microorganism center obtains the bacterial strain.
Bu Shi Bacillus acidi lactici (Lactobacillus buchneri) CGMCC NO.1.3114 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from Chinese Microbiological Culture Collection conservator
Meeting common micro-organisms center obtains the bacterial strain.
Colloid bacillus cereus (Bacillus mucilaginosus) CGMCC NO.1.3114 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from Chinese Microbiological Culture Collection conservator
Meeting common micro-organisms center obtains the bacterial strain.
Rihizobium japonicum (Bradyrhizobium japonicum) CGMCC NO.1.2550 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from Chinese Microbiological Culture Collection conservator
Meeting common micro-organisms center obtains the bacterial strain.
Mostly viscous class gemma (Paenibacillus polymyxa) CGMCC NO.1.4261 is preserved in Chinese microorganism strain
Preservation administration committee common micro-organisms center (abbreviation CGMCC), the public can be from China Committee for Culture Collection of Microorganisms
Common micro-organisms center obtains the bacterial strain.
Embodiment 1: the preparation of basic " saline-alkali tolerant composite flora ":
The present invention discards the saltern growth fluffy rhizosphere of alkali from Binhai New Area in Tianjin and takes native separate microorganism；More than a century
Under 352 plants of Age-old pine trees of Changbai Mountain beauty pine tree in rhizosphere vegetable mould, fertile black earth is taken, carries out microorganism fungus kind separation screening,
So that breeding useful plants grow microorganism；From soybean nodulation, microorganism fungus kind separation screening is carried out, so that breeding is beneficial to plant
Object grows microorganism；Like to take fertile black earth in the reed rhizosphere soil of late lake, carry out from the University Of Tianjin with healthy and strong reed for many years
Microorganism fungus kind separation screening, so as to breeding degradation environmental pollution and (separating screening method is shown in ginseng to useful plants growth microorganism
Examine document: azotobacter chroococcum, the production of bacillus megaterium composite bacterial fertilizer and application effect [J] Agriculture of Anhui science, 2010,
38 (28): 15705-15706；Selecting Bacterial Strains for Use in the Biocontrol of
Diseases Caused by Phytophthora capsici and Alternaria alternata in Sweet
Pepper Plants [J] Blologia Plantarum, 2003,47 (4): 569-574；Bacillus amyloliquefaciens fermentation training
The design of base and optimization [J] the Agriculture of Anhui science of fermentation condition are supported, 2010,38 (18): 9402-9405,9437).
Firstly, the material of above-mentioned 3 ground acquisition is formed " saline-alkali tolerant composite flora ".
In view of not increasing the metal ion burden of soil, culture medium of our autogamys based on degradable foodstuff.
Above-mentioned saline-alkali tolerant composite flora is taken, " agriculture is residual with the formation such as straw powder, meat soup, agar, potato, sucrose, brewer's wort, bean sprout juice
Culture medium " is cultivated (formula of culture medium is shown in embodiment 2), and main effect flora is formed, and is irrigated and (is filled in plant roots such as corns
Irrigate bacterial content 10 in liquid3-105Cfu/g), meanwhile, spray for the plant leaf blades such as corn (bacterial content 10 in spray liquid3-
105Cfu/g), the observation of the plant growths such as corn is carried out, it is found that this flora facilitates the plant growths such as corn.
Embodiment 2: the separation and identification of main effect bacterium
" saline-alkali tolerant composite flora " is taken to be diluted, with " the residual culture medium of agriculture ": with straw powder 0.1-100g/L, meat soup
(light), agar 1g/L, potato 10-200g/L, sucrose 1-30g/L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/L, phosphoric acid hydrogen two
Potassium 0.1-5g/L, yeast powder 0.1-6g/L, PH5-8.5 forms culture medium, after sterilized, is paved into plate, obtains monoclonal bacterial strain.
Experiment separates 121 plants of strain, wherein 69 plants of spore bacteria, and 20 plants of anaerobism spore bacteria, 10 plants of lactic acid bacteria, yeast
5 plants of bacterium, 3 plants of piece coccus, 4 plants of pseudomonad, 4 plants of actinomyces, 6 plants of mould.
It is expanded by 16SrDNA universal primer, send to sequencing company and be sequenced, identify 1 strain of table.
1 saline-alkali tolerant bacterial strain of table purifies screening and identification result
Embodiment 3: the preparation of bacterial manure carrier format and different conditions bacterial manure
There are three types of forms for bacterial manure carrier of the present invention, depending on customer demand:
(1) microorganism carrier in a dormant state: this form is easy to save, microbial spore or phanerozoic microorganism and white clouds
The material mixings such as stone or calcium carbonate or conch meal, living bacteria count are lower than liquid microbe fertilizer living bacteria count, the micro- life of disadvantage
Object needs to activate, and most of space is occupied by dolomite or carbonic acid calcium substance, needs to release microorganism by irrigation and rainwater
It releases, perhaps influences the using effect of fertilizer.
(2) dry or gel starch bacterial manure, most of available bio-feritlizers are deposited in the form of starch currently on the market
?.Microorganism can be in a kind of dormant state within the regular hour, and before they contact soil, will can gradually form sediment
Powder and gel consume, and the fertilizer of starch form has the advantages of bacterial strain keeps vitality, but may have higher pollution.
(3) bacterial manure of liquid form, advantage are microbe densities than powdered much higher, microbial cells activity height.
But microorganism is not in dormant state in this kind of fertilizer, and just vie each other living space and battalion between a variety of strains
Support substance.Therefore, the growth of some microorganisms will be suppressed.The difference of this kind of bio-feritlizer and powdered biological fertilizer is just
It is with the presence or absence of the effect of confronting with each other between microorganism.
Above 3 kinds of forms are all bacterial manure common types of the present invention, can according to demand be prepared carrier and following methods
Microbial inoculum mixture, which mixes, to be formulated.
Embodiment 4: saline-alkali tolerant growth promotion composite bacteria agent A bacterial screening, culture and its compounding
1 bacterial strain of table is used into " straw powder (or 5 centimetres or less particles after corruption) 0.1-100g/L, meat soup (light), horse respectively
Bell potato 10-200g/L, sucrose 1-30g/L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/L, dipotassium hydrogen phosphate 0.1-5g/L, yeast
For powder 0.1-6g/L, PH5-8.5 " fluid nutrient medium as bacterial manure carrier, fermented and cultured is in office when bacterium solution OD value reaches 0.5-1.2
What plant leaf blade, root largely spray, and spray plant group, control group is 17-30 and is detected, and cannot at least be less than 3 plants,
It is the more the better.Observation of plant growing state, record plant characterization.It selects by contrast, it is found that bacillus megaterium, waxy gemma
Bacillus, bacillus subtilis, bacillus amyloliquefaciens, Paenibacillus polymyxa, Trichoderma harzianum, colloid bacillus cereus, Soybean Root
Tumor bacterium and Bu Shi Bacillus acidi lactici etc. have apparent growth-promoting effect in bacterial manure carrier of the present invention, to plant, are this carrier bacteriums
Fertile saline-alkali tolerant growth promotion composite bacteria agent A main effect bacterium screening.
2 saline-alkali tolerant A group growth promotion bacterial manure strain of table
Saline-alkali tolerant growth promotion composite bacteria agent A group growth promotion bacterial manure strain is cultivated respectively in above-mentioned culture medium, and compounding forms A
It is rihizobium japonicum rihizobium japonicum described in class microbial inoculum (Bradyrhizobium japonicum) CGMCC NO. 1.2550, huge
Bacterium anthracoides (Bacillus megaterium) CGMCC NO.1.10466, bacillus cereus (Bacillus
Cereus) CGMCC NO.1.10559, bacillus subtilis (Bacillus subtilis) CGMCC NO.1.4255, colloid bud
Spore bacillus (Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus
Buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC
NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa) CGMCC NO.1.4261 and Trichoderma harzianum
The bacterium colony of (Trichoderma harzianum) CGMCC NO.3.6604 forms living bacteria count (CFU) than being (4.3~6.5)
: (4.3~6.5): (4.3~6.5): (3.2~5): (3.2~5): (3.2~5): the microbial inoculum of (3.2~5).Specifically finely match
Ratio, need to be because of the different plant of crop, vegetables, fruit tree and flowers etc. depending on.
The diseases prevention of embodiment 5:B group, microbes producing cellulase bacterial manure bacterial screening, culture and its compounding
1 bacterial strain of table is used into " straw powder (or 5 centimetres or less particles after corruption) 0.1-100g/L, meat soup (light), horse respectively
Bell potato 10-200g/L, sucrose 1-30g/L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/L, dipotassium hydrogen phosphate 0.1-5g/L, yeast
Powder 0.1-6g/L, PH5-8.5 " fluid nutrient medium makees fermented and cultured, when bacterium solution OD value reaches 0.3-2.5, any plant leaf blade,
Root largely sprays, and sprays plant group, control group is 17-30 and is detected, and cannot at least be less than 3 plants, The more the better.It sees
Plant is examined in the plantation growing state of pathogen, records plant pest characterization.It was found that bacillus pumilus, lichens bud
Spore bacillus, colloid bacillus cereus, Bu Shi Bacillus acidi lactici etc., bacillus amyloliquefaciens, Paenibacillus polymyxa, Trichoderma harzianum etc. exist
In bacterial manure carrier of the present invention, there are apparent diseases prevention, zymogenesis to plant, is this carrier bacterial manure saline-alkali tolerant diseases prevention producing enzyme compound bacteria
Agent B strain (being shown in Table 3).
3 B group saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent bacterial screening of table
Culture compounding forms bacillus pumilus to saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B strain respectively in above-mentioned culture medium
(Bacillus pimilus) CGMCC NO.1.10291, bacillus licheniformis (Bacillus licheniformis) CGMCC
NO.1.884, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici
(Lactobacillus buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa) CGMCC
The bacterium colony of NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604 form effective viable bacteria
Number (CFU) is than being (4.3~6.5): (4.3~6.5): (4.3~6.5): (3.2~5): (3.2~5): (3.2~5): (3.2
~5) microbial inoculum.
It is specific finely to match ratio, need to be because of the different plants of crop, vegetables, fruit tree and flowers etc. depending on.
Embodiment 6: functional, biocontrol water saline-alkali tolerant bacterial manure preparation
By A group saline-alkali tolerant growth promotion bacterial manure microbial inoculum and the diseases prevention of B group saline-alkali tolerant, microbes producing cellulase bacterial manure microbial inoculum with 1: 1 ratio
It mixes, the microbial inoculum after taking 1-500ml to mix, 1000ml is added, and " straw powder (or 5 centimetres or less particles after corruption) is with stalk
Powder 0.1-100g/L, meat soup (light), potato 10-200g/L, sucrose 1-30g/L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/
L, dipotassium hydrogen phosphate 0.1-5g/L, fermented and cultured obtains saline-alkali tolerant bacterial manure fermentation temperature in yeast powder 0.1-6 g/L, PH5-8.5 "
50 degree；It mixes with a large amount of stalks or animal excrements etc., the temperature of fermented and cultured are as follows: temperature rise period: is raised to from room temperature later
It is 50 DEG C, general only to need one day；Hot stage: it is needed 2 days from 50-70 DEG C general.Temperature-fall period: from high-temperature drop to 50 DEG C with
Under, general 15 days or so, stalk fertilizer process was basically completed at this time, and fertilizer can be applied directly.
Embodiment 7: range of functional, biocontrol water saline-alkali tolerant bacterial fertilizer products and bacterial manure carrier
(1) by " microbial inoculum product " in above-mentioned 6 and earthworm Fertilizer combination, solid bacterial manure is formed.
(2) " microbial inoculum product " in above-mentioned 6 is formed into the fertilizer in the form of starch, to keep bacterial manure bacterial strain vitality, preparation
At dry or gel starch bacterial manure.
(3) by " microbial inoculum product " in above-mentioned 6 and " straw powder 0.1-100g/L, meat soup (light), ammonium sulfate 0.1-5g/L, horse
Bell sweet potato starch 3-15g/L, dipotassium hydrogen phosphate 0.1-5g/L, yeast powder 0.1-6g/L, PH7.0 ", form the bacterial manure of liquid form.
(4) by the agricultures maimed person such as " microbial inoculum product " and stalk material in above-mentioned 6 (peanut cake perhaps soya-bean cake or cottonseed cake, or
Other defective materials of person or rice husk etc. septage or above-mentioned substance mixing) form solid bacterial manure.This bacterial manure also solves straw
The environmental pollutions such as stalk.
(5) " microbial inoculum product " in above-mentioned 6 is formed into solid bacterial manure in conjunction with turf.
(6) by conjunction with " microbial inoculum product " in above-mentioned 6 and flower soil etc., peat, solid bacterial manure is formed.
(7) " microbial inoculum product " in above-mentioned 6 is sprayed into condensation in the material mixings such as dolomite or calcium carbonate or conch meal, is formed
The solid bacterial manure of microorganism in a dormant state.
(8) " microbial inoculum product " and pig, cattle and sheep chicken manure etc. in above-mentioned 6 are combined, forms solid bacterial manure.
(9) " microbial inoculum product " in above-mentioned 6 is directly mixed and stirred with various seeds, forms kind of a clothing.
According to demand, functionality, the biocontrol water saline-alkali tolerant bacterial manure for not only forming above-mentioned series, can also form any form
Bacterial manure.No matter any product, guarantee quality it is compound even better than microbial-bacterial fertilizer national standard.(GB according to national standards
Living bacteria count specified in 20287-2006) is respectively as follows: liquid more than or equal to 200,000,000/ml, and pulvis is more than or equal to 200,000,000/g, particle
More than or equal to 100,000,000/g.
Embodiment 8: saline-alkali tolerant bacterial manure of the present invention facilitates growth, output increased
(1)-(8) kind carrier in " microbial inoculum product " in above-mentioned 6 and above-described embodiment 7 is mixed into the novel fertilizer being prepared
Material,
Experiments have shown that it is bright that application saline-alkali tolerant bacterial manure of the present invention can promote all plant growth production-increasing effects compared with conventional fertilizer application
Aobvious, plant includes cereal crops: rice, corn, beans, potato, highland barley, semen viciae fabae, wheat etc.；Oil crops: oilseeds, climing
Green, big mustard, peanut, flax, hemp, sunflower etc.；Vegetable crop: radish, Chinese cabbage, celery, leek, garlic, green onion, carrot, dish
Melon, lotus flower dish, jerusalem artichoke, sword bean, coriander, asparagus lettuce, chrysanthemum, capsicum, cucumber, tomato, caraway etc.；Fruit: pears, green plum, apple
The kinds such as fruit, peach, apricot, walnut, plum, cherry, strawberry, Chinese pear-leaved crabapple, jujube；Wild fruit: sour pears, wild apricot, wild peach, mountain jujube, mountain
Cherry, sea-buckthorn etc.；Forage crop: clover, corn, green manure, Chinese milk vetch etc.；Medicinal crop: honeysuckle, ginseng, Radix Angelicae Sinensis, gold
Honeysuckle flower, peppermint, argy wormwood etc..And this product avoids is excessively used the decline of soil fertility caused by chemical fertilizer, crop quality for a long time
It deteriorates, environment is contaminated.Novel organic fertilizer that is pollution-free, being conducive to culture soil soil fertility.Bacterium in saline-alkali tolerant bacterial manure of the present invention
The organic matter that strain metabolism generates is the basis of fertility, meanwhile, and be the matrix of beneficial microbe culture, the agriculture of selection is residual, food is residual,
Environmental contaminants etc. have obtained utilizing and degrading again, protect environment.
Application effect report by taking Chinese cabbage as an example.Chinese cabbage is cultivated in illumination box, one group of application bacterial manure of the present invention is right
According to a group application urea, it is experimental field vegetable garden sandy soil that two groups of fertilizer, which are routinely applied, is applied before the processing field planting of nutrition seedlings and soil
60% fertilizer amount, remaining 40% divides equally 3 parts, is sprayed with 10 times of water retting liquid in the phase of turning green, rosette state and balling initial stage blade face
Mist.Conventional fertilizer application is got used to the phase of turning green, rosette state by locality and is applied down for balling initial stage 3 times, observation organic fungi-manure to Chinese cabbage growth,
The influence of yield and quality.The preparation method of the present embodiment bacterial manure is to mix A class microbial inoculum and B class microbial inoculum with 1: 1 ratio, is taken mixed
Microbial inoculum after even is added the residual culture medium fermented and cultured of agriculture and obtains bacterial manure or bacterium product；
The residual culture medium 0.1-100g/L containing straw powder of the agriculture, meat soup (light), potato 10-200g/L, sucrose 1-30g/
L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/L, dipotassium hydrogen phosphate 0.1-5g/L and yeast powder 0.1-6g/L, PH5-8.5；
A class microbial inoculum rihizobium japonicum rihizobium japonicum (Bradyrhizobium japonicum) CGMCC NO.
1.2550, bacillus megaterium (Bacillus megaterium) CGMCC NO.1.10466, bacillus cereus
(Bacillus cereus) CGMCC NO.1.10559, bacillus subtilis (Bacillus subtilis) CGMCC
NO.1.4255, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici
(Lactobacillus buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa) CGMCC
The bacterium colony of NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604 form living bacteria count
(CFU) than being 4.3: 4.3: 4.3: 3.2: 3.2: 3.2: 3.2.
Bacillus pumilus (Bacillus pimilus) CGMCC NO.1.10291, bacillus licheniformis in B class microbial inoculum
(Bacillus licheniformis) CGMCC NO.1.884, colloid bacillus cereus (Bacillus mucilaginosus)
CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus buchneri) CGMCC NO.1.3114, solution starch bud
Spore bacillus (Bacillus amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma (Paenibacillus
Polymyxa) the bacterium of CGMCC NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604
It falls to form living bacteria count (CFU) than being 4.3: 4.3: 4.3: 3.2: 3.2: 3.2: 3.2.
Growth and yield observation:
The Chinese cabbage growth for applying bacterial manure treatment region of the present invention is very fast, and yield is higher.Investigation result is shown, applies bacterium of the present invention
Fertile growing way is significantly better than application urea.
The saline-alkali tolerant bacterial manure of the present invention of table 4 is to Chinese cabbage growth, the influence of yield
| Treatment region | Plant height (cm) | Leaf width (cm) | The number of blade | Weight per plant (kg) |
| Junf1 (bacterial manure) | 35.1±0.30 | 49.7±0.50 | 15.7±0.30 | 1.9±0.23 |
| Junf2 (bacterial manure) | 37.0±0.80 | 48.5±0.70 | 18.9±0.40 | 1.7±0.10 |
| Junf3 (bacterial manure) | 33.8±0.40 | 46.2±0.60 | 22.8±0.30 | 1.8±0.20 |
| Ns1 (urea) | 31.5±0.40 | 39.5±0.30 | 16.2±0.20 | 1.4±0.30 |
| Ns2 (urea) | 34.6±0.50 | 40.2±0.50 | 14.5±0.40 | 0.9±0.15 |
| Ns3 (urea) | 29.5±0.60 | 42.1±0.60 | 13.8±0.50 | 0.8±0.23 |
Saline-alkali tolerant bacterial manure of the present invention is significantly better than urea control group in terms of the cell production of harvest, reaches significant difference water
It is flat.
Influence of the saline-alkali tolerant bacterial manure of the present invention to Agronomic Characters In Corn is also very big, in table 5 after 2 kinds of fertilization mode processing
Corn plant height, Ear height, stem thickness, root long, plant dry mass and Fresh Yuxincao are gradually increased with corn growth, and Ear height is insufficient
The half of plant height.Plant in three breeding times, plant height, the single plant for applying bacterial manure and control group processing plant are green
The number of sheets, root long, dry mass, Fresh Yuxincao apply bacterial manure obviously than control without significant difference, but in tasseling stage, pustulation period in the jointing stage
Group is there are advantage, and significant difference.Stem thickness, lateral root number apply bacterial manure equal table compared with the control group within three growth periods of corn
Reveal significant difference, Ear height then in the tasseling stage and pustulation period that it occurs, applies bacterial manure group than control group plant fringe position
High 7-9cm, and significant difference.
5 Different Fertilization mode of table Agronomic Characters In Corn within corn each breeding time compares
Embodiment 9: saline-alkali tolerant bacterial manure of the present invention facilitate prevention by pythium spp, Rhizoctonia solani Kuhn, sickle-like bacteria, bread mold,
Phytopathy caused by the pathogens such as column spore is mould, sclerotinite, Sclerotium rolfsii is fallen ill
By the bacterial manure prepared in embodiment 8 and implement the new-type fertilizer that the mixing of 1-8 kind carrier is prepared in 7, test knot
Fruit shows that compared with conventional fertilizer application, the pre- preventing disease and pest of all test plants can be made by applying bacterial manure of the present invention, and the third effect is obvious.It is receiving
The later period is obtained, all plants have disease-resistant, effect of increasing production, and the plant includes: cereal crops: rice, corn, beans, potato, blueness
Highland barley, semen viciae fabae, wheat etc.；Oil crops: oilseeds, turnip, big mustard, peanut, flax, hemp, sunflower etc.；Vegetable crop:
Radish, Chinese cabbage, celery, leek, garlic, green onion, carrot, snake melon, lotus flower dish, jerusalem artichoke, sword bean, coriander, asparagus lettuce, chrysanthemum, capsicum, Huang
Melon, tomato, caraway etc.；Fruit: the product such as pears, green plum, apple, peach, apricot, walnut, plum, cherry, strawberry, Chinese pear-leaved crabapple, jujube
Kind；Wild fruit: sour pears, wild apricot, wild peach, mountain jujube, downy cherry fruit, sea-buckthorn etc.；Forage crop: clover, corn, green manure, purple cloud
English etc.；Medicinal crop: honeysuckle, ginseng, Radix Angelicae Sinensis, honeysuckle, peppermint, argy wormwood etc..It is reachable to the field efficacy of bacterial wilt
71~96%, with morbidity control group highest rate of growth up to 493%；To the soil-borne diseases such as damping-off and capsicum epidemic disease also have compared with
Good preventive and therapeutic effect.
Saline-alkali tolerant bacterial manure of the present invention facilitates plant disease-resistant, and soil microbe quantity is that reflection soil quality is horizontal and healthy
The important indicator of degree, these microorganisms participate in substance circulation and energy metabolism.Experiment takes plant rhizosphere 0-20cm native
Earth measures the quantity of its bacterium, fungi, actinomyces, and the results are shown in Table 6, and applying bacterial manure changed dramatically bacterium in soil, true
The quantity of bacterium, actinomyces, and it is different to different microbiological effect effects.Compared with the control, apply bacterial manure after, bacterium and
Population of Actinomycetes has increased separately 302.65% and 64.47%, and quantities of soil microbe increases 274.56%, and difference is aobvious
It writes.And fungi applies bacterial manure group and is lower than control group, compared with the control, soil fungi quantity reduces 67.9%.
Influence of the 6 Different Fertilization mode of table to three categories microorganism in corn soil
| Processing mode | Bacterium/105cfu·g-1 | Fungi/103cfu·g-1 | Actinomyces/105cfu·g-1 | Microbial count/105cfu·g-1 |
| Bacterial manure | 24.95 ± 0.05* | 0.37 ± 0.005* | 1.23 ± 0.12* | 34.52 ± 0.52* |
| Control | 9.68±0.02 | 0.82±0.001 | 0.85±0.07 | 9.67±0.23 |
Claims (7)
1. the preparation method of a kind of saline-alkali tolerant bacterial manure or saline-alkali tolerant bacterium product, it is characterised in that: by saline-alkali tolerant growth promotion compound bacteria
Agent A and saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B are with 1: (0.1-10) ratio mixes, and takes the microbial inoculum after mixing, it is residual that agriculture with high salt is added
Culture medium obtains bacterial manure or bacterium product；
The residual culture medium 0.1-100g/L containing straw powder of the agriculture with high salt, NaCl 5-27g/L, meat soup (light), agar 1g/L, Ma Ling
Potato 10-200g/L, sucrose 1-30g/L, bean powder 1-15g/L, ammonium sulfate 0.1-5g/L, dipotassium hydrogen phosphate 0.1-5g/L, yeast powder
0.1-6g/L, PH7.5-9.5；
The active constituent of the saline-alkali tolerant growth promotion bacterium composite bacteria agent A is by rihizobium japonicum (Bradyrhizobium
Japonicum) CGMCC NO.1.2550, bacillus megaterium (Bacillus megaterium) CGMCC NO.1.10466,
Bacillus cereus (Bacillus cereus) CGMCC NO.1.10559, bacillus subtilis (Bacillus subtilis)
CGMCC NO.1.4255, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi lactic acid
Bacillus (Lactobacillus buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens (Bacillus
Amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa) CGMCC
NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604 composition；
The active constituent of the saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B is by bacillus pumilus (Bacillus pimilus)
CGMCC NO.1.10291, bacillus licheniformis (Bacillus licheniformis) CGMCC NO.1.884, colloid gemma
Bacillus (Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus
Buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC
NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa) CGMCC NO.1.4261 and Trichoderma harzianum
(Trichoderma harzianum) CGMCC NO.3.6604 composition.
2. according to the method described in claim 1, it is characterized by: soybean nodulation described in saline-alkali tolerant growth promotion composite bacteria agent A
Bacterium (Bradyrhizobium japonicum) CGMCC NO.1.2550, bacillus megaterium (Bacillus megaterium)
CGMCC NO.1.10466, bacillus cereus (Bacillus cereus) CGMCC NO.1.10559, bacillus subtilis
(Bacillus subtilis) CGMCC NO.1.4255, colloid bacillus cereus (Bacillus mucilaginosus) CGMCC
NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus buchneri) CGMCC NO.1.3114, bacillus amyloliquefaciens
(Bacillus amyloliquefaciens) CGMCC NO.1.936, mostly viscous class gemma (Paenibacillus polymyxa)
The bacterium colony of CGMCC NO.1.4261 and Trichoderma harzianum (Trichoderma harzianum) CGMCC NO.3.6604 are formed effectively
Viable count (CFU) is than being (4.3~6.5): (4.3~6.5): (4.3~6.5): (3.2~5): (3.2~5): (3.2~5):
(3.2~5).
Bacillus pumilus (Bacillus pimilus) CGMCC in the saline-alkali tolerant diseases prevention producing enzyme composite bacteria agent B
NO.1.10291, bacillus licheniformis (Bacillus licheniformis) CGMCC NO.1.884, colloid bacillus cereus
(Bacillus mucilaginosus) CGMCC NO.1.3114, Bu Shi Bacillus acidi lactici (Lactobacillus buchneri)
CGMCC NO.1.3114, it bacillus amyloliquefaciens (Bacillus amyloliquefaciens) CGMCC NO.1.936, glues more
Class gemma (Paenibacillus polymyxa) CGMCC NO.1.4261 and Trichoderma harzianum (Trichoderma
Harzianum) bacterium colony of CGMCC NO.3.6604 forms living bacteria count (CFU) than being (4.3~6.5): (4.3~6.5):
(4.3~6.5): (3.2~5): (3.2~5): (3.2~5): (3.2~5).
The volume ratio of microbial inoculum and the residual culture medium of agriculture with high salt after the mixing is 1-500: 1000.
3. the bacterial manure or bacterium product that be prepared by method as claimed in claim 1 or 2.
4. bacterial manure as claimed in claim 3 or bacterium product are promoting plant growth or are improving in crop yield or planting soil improvement
Application.
5. bacterial manure as claimed in claim 3 or bacterium product are improving the application in crop disease-resistant ability.
6. application according to claim 4 or 5, it is characterised in that: the crop is following any: rice, corn,
Beans, potato, highland barley, semen viciae fabae, wheat, oilseeds, turnip, big mustard, peanut, flax, hemp, sunflower, radish, Chinese cabbage, celery,
Leek, garlic, green onion, carrot, snake melon, lotus flower dish, jerusalem artichoke, sword bean, coriander, asparagus lettuce, chrysanthemum, capsicum, cucumber, tomato, caraway,
Pears, green plum, apple, peach, apricot, walnut, plum, cherry, strawberry, Chinese pear-leaved crabapple, jujube, sour pears, wild apricot, wild peach, mountain jujube, downy cherry fruit,
Sea-buckthorn, clover, corn, green manure, Chinese milk vetch, honeysuckle, ginseng, Radix Angelicae Sinensis, honeysuckle, peppermint and argy wormwood.
7. application according to claim 4 or 5, the disease is as caused by bacterium, fungi or actinomycotic infection.
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