CN101928161B - Method for preparing direct seeding compression nutrition pot raw material - Google Patents
Method for preparing direct seeding compression nutrition pot raw material Download PDFInfo
- Publication number
- CN101928161B CN101928161B CN2010102716126A CN201010271612A CN101928161B CN 101928161 B CN101928161 B CN 101928161B CN 2010102716126 A CN2010102716126 A CN 2010102716126A CN 201010271612 A CN201010271612 A CN 201010271612A CN 101928161 B CN101928161 B CN 101928161B
- Authority
- CN
- China
- Prior art keywords
- preparation
- hours
- fermention medium
- composite fungus
- fungus agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a method for preparing a direct seeding compression nutrition pot raw material. The method comprises the following steps of: inoculating 8 to 12 percent of mycete compound microbial inoculum, 4 to 6 percent of yeast compound microbial inoculum, 6 to 10 percent of azotobacter compound microbial inoculum and 4 to 6 percent of bacillus subtilis compound microbial inoculum into a fermentation culture medium sequentially, and stirring the culture medium repeatedly; and after the fermentation is finished, drying at the temperature of between 40 and 45 DEG C under the vacuum of 0.1 MPa until the moisture is less than or equal to 8 percent, and packaging to obtain products. The direct seeding compression nutrition pot raw material prepared by the method contains bactericides and various nutritional components which are required by the seedling emergence and growth of sprouts, and can ensure the whole seedling and strong seedling of direct root system plants.
Description
Technical field
The present invention relates to a kind of preparation method of direct seeding compression nutrition pot raw material, the preparation method of the direct seeding compression nutrition pot raw material that particularly a kind of activeconstituents is made up of multiple microbiobacterial agent.
Background technology
For taproot system class plant (like cotton, rape etc.); In planting process, need transplant (asking letter to chat the reason of seedling replanting) to seedling in order to solve the big sowing difficulty of cultivated area, saving seed, raising output etc.; Because the transplanting process is lost seedling easily; And labour intensity is big, therefore in seedling raising process, constantly explores new seedling method.Late 1980s, " nutrition bowl seedling transplanting " technology has obtained widespread use in the cotton planting process.Report the technology that cotton nutrition is grown seedlings such as, Xu Xunyuan (" cotton nutrition is grown seedlings and cultivated technology in early complete neat strong sprout ", the 29th the 2nd phase of volume of " Jiangxi cotton " April in 2007), comprised the preparation of Nursery, the step of sowing and transplant seedlings etc.Yet should technology problems such as labour intensity is big, efficient is low, recruitment is many, cost height in production practice, have also been exposed.Therefore; Cotton Inst., Chinese Agricultural Academy has carried out the research of cotton substrate seedling transplantation technique in Tenth Five-Year Plan Period; And obtained breakthrough; This technology is compared with the nutrition pot seedling transplantation technique, and the breakthrough of four aspects is arranged, and the seedlings and soil of having corrected one's mistakes is a non-soil culture, the carrying agent of having corrected one's mistakes (being with soil) transplanting is transplanted for industrial seedling rearing, the manual work of correcting one's mistakes for the transplantation of seedlings of no carrying agent list, the single household of correcting one's mistakes disperse to grow seedlings is mechanization transplanting.Summarized the technology that the cotton substrate seedling is transplanted such as, people such as Yang Xingbai (" the cotton substrate seedling is transplanted the characteristics and management main points of new technology ", " 2007 the 6th phases of crop journal).Occurred in the demonstration that surviving rate is low, seedling-slowing stage long and problem such as cotton seedling is uneven yet should technology promote, had a strong impact on growing of cotton seedling, caused the decline of output of cotton and quality in the whole nation.
Chinese patent CN101058792A discloses a kind of highly effective straw decomposition composite flora; 14 kinds of bacterial classifications such as this composite flora is mould by Phanerochaete chrysosporium, Lentinus Edodes fungus, trichoderma harziarum, viride, healthy and free from worry wood, black mold, subtilis, feed genus bacillus, bacillus megaterium, candiyeast, distillery yeast, food-yeast, aztobacter sp, thermophilic actinomycete are formed; Xylogen, Mierocrystalline cellulose, semicellulose and other organic substances in the comprehensive degrading straw of ability, degradation rate is high.Yet the disclosed composite flora of the document only is used for decomposition composite flora, can not be used to need not the cotton seedling cultivation of seedling replanting.
Chinese patent CN101508601A discloses a kind of microbial organic fertilizer and preparation method thereof; Adopting agricultural byproducts tankage (grouts, fowl and animal excrement, stalk, rice bran, bone meal etc.) is raw material; Adopting known yeast and subtilis is beneficial microbe colony, produces bacterial classification through liquid fermenting, and one or both bacterial classifications of the heavy dose of inoculation of raw material are carried out solid fermentation; Become thoroughly decomposed after the multifunctional microbial organic fertilizer is processed in air-dry or oven dry.Yet the disclosed microbial organic fertilizer of the document only is used to improve quality of agricultural product and crop yield, and the physicochemical character of improving the soil reduces environmental pollution, can not be used to need not the cotton seedling cultivation of seedling replanting.
Chinese patent CN101361451A discloses a kind of paddy plant material cupulate compartment tray and preparation method thereof; This seedling disk material is made up of straw powder, water-soluble biological latex, little fertile nutrient media soil, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, sterilant and the acidity regulator compatibility of Different Weight, pulverizes, mixes and the technological process preparation such as stir, hot-forming and accomplish through rice straw.Yet the disclosed paddy plant material cupulate compartment tray of the document does not relate to any microbiobacterial agent, is not to utilize microbial fermentation to prepare fertilizer, and it is many to prepare the required raw material type of this seedling dish, and consumption is big, and cost is high, is not suitable for the big production of chemurgy.
Direct seeding compression nutrition pot is that seed is placed in the compression nutrition bowl, with the directly live a kind of novel cotton planting type to the land for growing field crops of machinery, has saved and has planted the time-consuming operation of transplantation of seedlings, has greatly alleviated labour intensity.But, realize effective direct seeding compression nutrition pot cultivation, must rely on the fine direct seeding compression nutrition pot raw material.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of direct seeding compression nutrition pot raw material, the preparation method of the direct seeding compression nutrition pot raw material that a kind of activeconstituents is made up of multiple microbiobacterial agent is provided especially.Utilizing the direct seeding compression nutrition pot raw material of this method preparation to contain seedling emerges and grows required sterilant and various nutrition; Can guarantee taproot system class plant full stand and strong sprout; Solved that Nursery transplants that early send out strong sprout but labour intensity is big, substrate seedling is transplanted low but the contradiction that early sends out difficult strong sprout of labour intensity, concentrated the advantage of above-mentioned two kinds of seedling raising mannerses and remedied their deficiency.
In order to realize the object of the invention, the contriver has finally all of a sudden obtained a kind of preparation method of direct seeding compression nutrition pot raw material through lot of experiments, and its preparation technology's flow process is following:
Complex micro organism fungicide → activation
↓
Stalk → pulverizing → batching → disinfection tank → fermenting tank for fermentation → vacuum-drying → packing → raw produce
Particularly, the preparation method of direct seeding compression nutrition pot raw material of the present invention in turn includes the following steps:
1) in fermention medium, insert the mould composite fungus agent of 8-12%, 30 ℃ of condition bottom fermentations 36-48 hour stir fermention medium in the fermenting process, and earlier fermentation whenever stirred once at a distance from 8 hours, fermented after 24 hours, whenever stirred once at a distance from 4 hours;
2) in fermention medium, insert the yeast composite fungus agent of 4-6%, 28 ℃ of condition bottom fermentations 16-24 hour stir fermention medium in the fermenting process, whenever stirred once at a distance from 4 hours;
3) in fermention medium, insert the vinelandii composite fungus agent of 6-10%,, stir fermention medium in the fermenting process, whenever stirred once at a distance from 3 hours 32 ℃ of condition bottom fermentations 24-36 hour;
4) in fermention medium, insert the bacillus subtilis microbial agent of 4-6%,, stir fermention medium in the fermenting process, whenever stirred once at a distance from 6 hours 37 ℃ of condition bottom fermentations 16-24 hour; After the fermentation ends, under the vacuum tightness of 0.1Mpa, temperature 40-45 ℃, be dried to moisture content≤8%.
The preparation method of above-mentioned direct seeding compression nutrition pot raw material, by weight, said fermention medium is made up of the water of stalk 28%, ammonium sulfate 3%, urea 0.5%, potassium primary phosphate 1%, sal epsom 0.3%, calcium chloride 0.2% and surplus.Preferably, said crushed stalk is to the fineness of crossing the 30-60 mesh sieve.
The preparation method of above-mentioned direct seeding compression nutrition pot raw material, the activeconstituents of said mould composite fungus agent is made up of black mold (Aspergillusniger) ATCC 16404, aspergillus oryzae (Aspergillus oryzae) CICC41478, viride (Trichoderma viride) CCTCC AF93252, geotrichum candidum (Geotrichum candidum) CICC31418.Four kinds of mikrobes (black mold, aspergillus oryzae, viride and geotrichum candidum) difference single culture of forming the mould composite fungus agent; And process the single strain preparation through solid state fermentation and cryodrying, four kinds of mikrobe single strain preparations obtain the mould compound formulation according to certain mixed after evenly.Preferably; The preparation process of said mould composite fungus agent is: in PDA solid slant culture base, be inoculated into respectively in the PDA liquid nutrient medium through twice activatory black mold, aspergillus oryzae, viride, geotrichum candidum bacterial classification; At 30 ℃; Cultivate under the rotating speed 180rpm condition, make spore count reach 2.5-6.0 * 10
8Individual/mL promptly gets seed liquor, by the inoculum size of 5-10% said seed liquor is inoculated into the substratum proportion of composing to be: stalk 90%, wheat bran 6%, (NH
4)
2SO
42%, urea 1%, KH
2PO
40.6%, MgSO
40.2%, CaCl
20.2%, moisture content is in the solid-state fermentation culture medium of total solids content 60-70%, and at 30 ℃, every at a distance from the condition bottom fermentation that turned over Qu Yici in 2-3 hour, tunning is vacuum-drying to moisture≤10% under 40 ℃ of conditions, and spore content 2.5-4 * 10
9Individual/gram, be the single strain preparation; With said black mold, aspergillus oryzae, viride, geotrichum candidum single strain preparation 3: 2: 3 by weight: 1 ratio uniform mixing promptly got the mould composite fungus agent.
The preparation method of above-mentioned direct seeding compression nutrition pot raw material, the activeconstituents of said yeast composite fungus agent is made up of cereuisiae fermentum (Saccharomyces cerevisiae) CICC31362, candida tropicalis (Candida tropicalis) CICC1779, Candida utilis (Candida utilis) ATCC9950.Three kinds of mikrobes (cereuisiae fermentum, candida tropicalis and Candida utilis) difference single culture of forming the yeast composite fungus agent; And process liquid single strain preparation through liquid fermenting, three kinds of mikrobe single strain preparations obtain the yeast compound formulation according to certain mixed after evenly.Preferably; The preparation process of said yeast composite fungus agent is: twice activatory cereuisiae fermentum of warp, candida tropicalis, Candida utilis bacterial classification inoculation are in the liquid nutrient medium that said perfect medium is housed in the solid slant culture base of the perfect medium of being made up of glucose 2%, yeast extract paste 1% and peptone 2%; At 28 ℃; Cultivate under the rotating speed 180rpm condition, make cell count reach 7-9 * 10
8Individual/mL promptly gets seed liquor; By the inoculum size of 4-6% said seed liquor is inoculated in the wort of sterilization that concentration is 8-15Bx, at 28 ℃, rotating speed 180rpm; Ventilation: be cultured to cell count 2.5-4.5 * 109/mL under the 0.3-1v/vmin condition, be the single strain preparation; Said cereuisiae fermentum, candida tropicalis, by volume 1: 1: 3 ratio uniform mixing of Candida utilis single strain preparation are promptly got the yeast composite fungus agent.
The preparation method of above-mentioned direct seeding compression nutrition pot raw material, the activeconstituents of said vinelandii composite fungus agent is made up of Bacillus foecalis alkaligenes (Alcaligenes faecalis) CICC23635 and blown-ball Azotobacter (Azotobacter chroococcum) CICC20603.Two kinds of mikrobes (Bacillus foecalis alkaligenes and blown-ball Azotobacter) difference single culture of forming the vinelandii composite fungus agent; And process liquid single strain preparation through liquid fermenting, two kinds of mikrobe single strain preparations obtain the vinelandii compound formulation according to certain mixed after evenly.Preferably; The preparation process of said vinelandii composite fungus agent is: in the solid slant culture base of Asbby nitrogen-free agar, be inoculated into respectively in the liquid nutrient medium that said Asbby nitrogen-free agar is housed through twice activatory Bacillus foecalis alkaligenes, blown-ball Azotobacter, at 32 ℃, cultivate under the rotating speed 180rpm condition; Make cell count reach 5-7 * 108/mL and promptly get seed liquor; Inoculum size by 6-8% is inoculated into said seed liquor in the Asbby nitrogen-free agar, at 32 ℃, and rotating speed 180rpm; Be cultured to cell count 3-5.5 * 109/mL under the air flow 0.5-1v/vmin condition, be the single strain preparation; Said Bacillus foecalis alkaligenes and by volume 1: 1 ratio uniform mixing of blown-ball Azotobacter single strain preparation are promptly got the vinelandii composite fungus agent.
The preparation method of above-mentioned direct seeding compression nutrition pot raw material; The preparation process of said subtilis (Bacillus subtilis) CICC23590 microbial inoculum is: twice activatory Bacillus subtilis strain of warp in the solid slant culture base of the basic medium of being made up of Carnis Bovis seu Bubali cream 0.3%, peptone 1% and NaCl 0.5%; Be inoculated in the liquid nutrient medium that said basic medium is housed,, cultivate under the rotating speed 150rpm condition at 37 ℃; Make cell count reach 2-3 * 109/mL and promptly get seed liquor; By the inoculum size of 4-6% said seed liquor being inoculated into substratum consists of: glucose 0.6g/100mL, and yeast powder 2g/100mL is in the fermention medium of Carnis Bovis seu Bubali cream 0.3g/100mL; At 37 ℃; Rotating speed 150rpm is cultured to cell count 22-32 * 109/mL under the air flow 0.3-0.7v/vmin condition, be bacillus subtilis microbial agent.
Compared with prior art, the preparation method of direct seeding compression nutrition pot raw material of the present invention has following beneficial technical effects:
1) preparation of direct seeding compression nutrition pot raw material employing stalk (like farm crop rhizome leaves such as cotton stalk, straw) is a raw material; Employing compound microorganism ferments technology; Break the compact crystalline structure that staple Mierocrystalline cellulose, semicellulose, xylogen combine and form in the stalk, make its loose expansion, be convenient to degraded and produce monose; Improved the content of protein (yeast body protein) in the product, nutritive ingredients such as the nitrogen in the raw material, phosphorus, potassium obtain good coordination.In addition, the subtilyne that produces in the subtilis thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, these active substances have the obvious suppression effect to the conditioned pathogen of pathogenic bacterium or autogenous infection.
2) adopt the direct seeding compression nutrition pot plant cotton to compare with nutrition bowl seedling transplanting; Can improve the output of cotton seedling quality and cotton; Can significantly reduce the labour intensity of cotton planting again, realize the mechanized operation of cotton planting better, have beneficial technical effects and remarkable economic efficiency.Particularly, can find out, adopt its plant height MV of direct seeding compression nutrition pot plant cotton than the short 1.3cm of nutrition bowl seedling transplanting control group MV, its better effects if by the test-results of embodiment 9 tables 1.Test-results by table 2 can be found out, adopts its individual plant fruit branch of direct seeding compression nutrition pot plant cotton to count MV and Duos 0.8 than nutrition bowl seedling transplanting control group MV, more helps the cotton result.Test-results by table 3 can be found out, adopts its cotton of direct seeding compression nutrition pot plant cotton to become bell to count MV and Duos 2.4 than nutrition bowl seedling transplanting control group MV, can improve output of cotton.Test-results by table 4 can be found out, adopts the heavy MV of its cotton bell of direct seeding compression nutrition pot plant cotton than the high 0.38g of nutrition bowl seedling transplanting control group MV, can improve output of cotton.
Embodiment
Below further describe the present invention through preferred embodiment, yet the scope of the invention is not limited only to the following example.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
The preparation of embodiment 1 mould composite fungus agent
1) the preparation process of black mold, aspergillus oryzae, viride, four kinds of single strain preparations of geotrichum candidum
((peeling potatoes is cut into piece and boils 30min yam 200g, uses filtered through gauze then at PDA; Get juice); Sucrose 20g adds water and is settled to 1000mL) in the solid slant culture base through twice activatory black mold, aspergillus oryzae, viride, geotrichum candidum bacterial classification inoculation in the triangular flask that the PDA liquid nutrient medium is housed, at 30 ℃; Cultivate under the rotating speed 180rpm condition, make spore count reach 2.5-6.0 * 10
8Individual/mL promptly gets seed liquor, by the inoculum size of 5-10% seed liquor is inoculated into the substratum proportion of composing to be: stalk 90%,, wheat bran 6%, (NH
4)
2SO
42%, urea 1%, KH
2PO
40.6%, MgSO
40.2%, CaCl
20.2%, moisture content is in the solid-state fermentation culture medium of total solids content 60-70%, and at 30 ℃, every at a distance from the condition bottom fermentation that turned over Qu Yici in 2-3 hour, tunning vacuum-drying to moisture≤10% under 40 ℃ of conditions is the single strain preparation.Microbial inoculum miospore content 2.5-4 * 10
9Individual/gram.
2) preparation of mould (black mold, aspergillus oryzae, viride, geotrichum candidum) composite fungus agent
With above-mentioned steps 1) four kinds of single strain preparations of black mold, aspergillus oryzae, viride, geotrichum candidum of preparing 3: 2: 3 by weight: 1 ratio uniform mixing promptly gets the mould composite fungus agent.
The preparation of embodiment 2 yeast composite fungus agents
1) the preparation process of cereuisiae fermentum, candida tropicalis, three kinds of single strain preparations of Candida utilis
At perfect medium (glucose 2%; Yeast extract paste 1%; Peptone 2%, pH nature) in the solid slant culture base through twice activatory cereuisiae fermentum, candida tropicalis, Candida utilis bacterial classification inoculation in the liquid nutrient medium triangular flask that perfect medium is housed, at 28 ℃; Cultivate under the rotating speed 180rpm condition, make cell count reach 7-9 * 10
8Individual/mL promptly gets seed liquor, by the inoculum size of 4-6% seed liquor is inoculated in the wort of sterilization that concentration is 8-15Bx, and at 28 ℃, rotating speed 180rpm, air flow: be cultured to cell count 2.5-4.5 * 10 under the 0.3-1v/vmin condition
9Individual/mL, be the single strain preparation of three primary yeasts.
2) preparation of yeast (cereuisiae fermentum, candida tropicalis, Candida utilis) composite fungus agent
With above-mentioned steps 1) by volume 1: 1: 3 the ratio uniform mixing of three kinds of single strain preparations of cereuisiae fermentum, candida tropicalis, Candida utilis for preparing promptly gets the yeast composite fungus agent.
The preparation of embodiment 3 vinelandii composite fungus agents
1) the preparation process of Bacillus foecalis alkaligenes, two kinds of single strain preparations of blown-ball Azotobacter
At Asbby nitrogen-free agar (N.F,USP MANNITOL 10g, lime carbonate 5g, potassium primary phosphate 0.2g; Sal epsom 0.2g sodium-chlor 0.2g, calcium sulfate 0.1g, zero(ppm) water 1000mL; PH6.8-7.0) be inoculated in the liquid nutrient medium triangular flask that the Asbby nitrogen-free agar is housed through twice activatory Bacillus foecalis alkaligenes, blown-ball Azotobacter in the solid slant culture base; At 32 ℃, cultivate under the rotating speed 180rpm condition, make cell count reach 5-7 * 10
8Individual/mL promptly gets seed liquor, by the inoculum size of 6-8% seed liquor is inoculated in the Asbby nitrogen-free agar, and at 32 ℃, rotating speed 180rpm, air flow: be cultured to cell count 3-5.5 * 10 under the 0.5-1v/vmin condition
9Individual/mL, be the single strain preparations of two kinds of vinelandii.
2) preparation of vinelandii (Bacillus foecalis alkaligenes, blown-ball Azotobacter) composite fungus agent
With above-mentioned steps 1) by volume 1: 1 the ratio uniform mixing of two kinds of single strain preparations of Bacillus foecalis alkaligenes, blown-ball Azotobacter for preparing promptly gets the vinelandii composite fungus agent.
The preparation of embodiment 4 bacillus subtilis microbial agents
At basic medium (Carnis Bovis seu Bubali cream 0.3%; Peptone 1%; NaCl 0.5%, pH nature) be inoculated in the liquid nutrient medium triangular flask that basic medium is housed, through twice activatory Bacillus subtilis strain in the solid slant culture base at 37 ℃; Cultivate under the rotating speed 150rpm condition, make cell count reach 2-3 * 10
9Individual/mL promptly gets seed liquor; By the inoculum size of 4-6% seed liquor being inoculated into substratum consists of: glucose 0.6g/100mL; Yeast powder 2g/100mL is in the fermention medium of Carnis Bovis seu Bubali cream 0.3g/100mL, at 37 ℃; Rotating speed 150rpm, air flow: be cultured to cell count 22-32 * 10 under the 0.3-0.7v/vmin condition
9Individual/mL, be bacillus subtilis microbial agent.
The preparation of embodiment 5 direct seeding compression nutrition pot raw materials
With crushed stalk to 30 purpose fineness, then by following proportional arrangement fermention medium (g/100g): stalk (butt): 28g, ammonium sulfate: 3g, urea: 0.5g, potassium primary phosphate: 1g, sal epsom: 0.3g, calcium chloride: 0.2g, water: 67g.After each component of substratum mixed, adopted steam sterilizings 20 minutes down at 121 ℃, be cooled to 40 ℃ subsequent use.Zymotechnique adopts stepwise fermentation; Its fermenting process is: the fs: in fermention medium, insert the mould composite fungus agent of 8% embodiment 1 preparation, behind the fermention medium uniform mixing, 30 ℃ of condition bottom fermentations 48 hours; Stir fermention medium in the fermenting process; Earlier fermentation whenever stirred once at a distance from 8 hours, fermented after 24 hours, whenever stirred once at a distance from 4 hours; Subordinate phase: fs fermentation is after 48 hours, in fermention medium, inserts the yeast composite fungus agent of 6% embodiment 2 preparations, 28 ℃ of condition bottom fermentations 20 hours, stirs fermention medium in the fermenting process, whenever stirs once at a distance from 4 hours; Phase III: subordinate phase fermentation is after 20 hours, in fermention medium, inserts the vinelandii composite fungus agent of 10% embodiment 3 preparations, 32 ℃ of condition bottom fermentations 28 hours, stirs fermention medium in the fermenting process, whenever stirs once at a distance from 3 hours; Stage: phase III fermentation is after 28 hours, in fermention medium, inserts the bacillus subtilis microbial agent of 4% embodiment 4 preparations, 37 ℃ of condition bottom fermentations 24 hours, stirs fermention medium in the fermenting process, whenever stirs once at a distance from 6 hours.After the fermentation ends, under the vacuum tightness of 0.1Mpa, temperature 40-45 ℃, be dried to moisture content≤8%, then the product after the packing.Through detecting, the protein contnt of direct seeding compression nutrition pot raw material product is 24-28%, and expansion rate of water absorption is 450-650%.
The preparation of embodiment 6 direct seeding compression nutrition pot raw materials
With crushed stalk to 60 purpose fineness, then by following proportional arrangement fermention medium (g/100g): stalk (butt): 28g, ammonium sulfate: 3g, urea: 0.5g, potassium primary phosphate: 1g, sal epsom: 0.3g, calcium chloride: 0.2g, water: 67g.After each component of substratum mixed, adopted steam sterilizings 20 minutes down at 121 ℃, be cooled to 40 ℃ subsequent use.Zymotechnique adopts stepwise fermentation; Its fermenting process is: the fs: in fermention medium, insert the mould composite fungus agent of 12% embodiment 1 preparation, behind the fermention medium uniform mixing, 30 ℃ of condition bottom fermentations 36 hours; Stir fermention medium in the fermenting process; Earlier fermentation whenever stirred once at a distance from 8 hours, fermented after 24 hours, whenever stirred once at a distance from 4 hours; Subordinate phase: fs fermentation inserted 4% yeast composite fungus agent after 36 hours in fermention medium, 28 ℃ of condition bottom fermentations 24 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 4 hours; Phase III: subordinate phase fermentation inserted 6% vinelandii composite fungus agent after 24 hours in fermention medium, 32 ℃ of condition bottom fermentations 30 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 3 hours; Stage: phase III fermentation inserted 6% bacillus subtilis microbial agent after 30 hours in fermention medium, 37 ℃ of condition bottom fermentations 16 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 6 hours.After the fermentation ends, under the vacuum tightness of 0.1Mpa, temperature 40-45 ℃, be dried to moisture content≤8%, then the product after the packing.Through detecting, the protein contnt of direct seeding compression nutrition pot raw material product is 24-28%, and expansion rate of water absorption is 450-650%.
The preparation of embodiment 7 direct seeding compression nutrition pot raw materials
With crushed stalk to 30-60 purpose fineness, then by following proportional arrangement fermention medium (g/100g): stalk (butt): 28g, ammonium sulfate: 3g, urea: 0.5g, potassium primary phosphate: 1g, sal epsom: 0.3g, calcium chloride: 0.2g, water: 67g.After each component of substratum mixed, adopted steam sterilizings 20 minutes down at 121 ℃, be cooled to 40 ℃ subsequent use.Zymotechnique adopts stepwise fermentation; Its fermenting process is: the fs: in fermention medium, insert the mould composite fungus agent of 10% embodiment 1 preparation, behind the fermention medium uniform mixing, 30 ℃ of condition bottom fermentations 42 hours; Stir fermention medium in the fermenting process; Earlier fermentation whenever stirred once at a distance from 8 hours, fermented after 24 hours, whenever stirred once at a distance from 4 hours; Subordinate phase: fs fermentation inserted 5% yeast composite fungus agent after 42 hours in fermention medium, 28 ℃ of condition bottom fermentations 20 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 4 hours; Phase III: subordinate phase fermentation inserted 8% vinelandii composite fungus agent after 20 hours in fermention medium, 32 ℃ of condition bottom fermentations 36 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 3 hours; Stage: phase III fermentation inserted 5% bacillus subtilis microbial agent after 36 hours in fermention medium, 37 ℃ of condition bottom fermentations 18 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 6 hours.After the fermentation ends, under the vacuum tightness of 0.1Mpa, temperature 40-45 ℃, be dried to moisture content≤8%, then the product after the packing.Through detecting, the protein contnt of direct seeding compression nutrition pot raw material product is 24-28%, and expansion rate of water absorption is 450-650%.
The preparation of embodiment 8 direct seeding compression nutrition pot raw materials
With crushed stalk to 30-60 purpose fineness, then by following proportional arrangement fermention medium (g/100g): stalk (butt): 28g, ammonium sulfate: 3g, urea: 0.5g, potassium primary phosphate: 1g, sal epsom: 0.3g, calcium chloride: 0.2g, water: 67g.After each component of substratum mixed, adopted steam sterilizings 20 minutes down at 121 ℃, be cooled to 40 ℃ subsequent use.Zymotechnique adopts stepwise fermentation; Its fermenting process is: the fs: in fermention medium, insert the mould composite fungus agent of 8% embodiment 1 preparation, behind the fermention medium uniform mixing, 30 ℃ of condition bottom fermentations 36 hours; Stir fermention medium in the fermenting process; Earlier fermentation whenever stirred once at a distance from 8 hours, fermented after 24 hours, whenever stirred once at a distance from 4 hours; Subordinate phase: fs fermentation inserted 5% yeast composite fungus agent after 36 hours in fermention medium, 28 ℃ of condition bottom fermentations 16 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 4 hours; Phase III: subordinate phase fermentation inserted 9% vinelandii composite fungus agent after 16 hours in fermention medium, 32 ℃ of condition bottom fermentations 32 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 3 hours; Stage: phase III fermentation inserted 5% bacillus subtilis microbial agent after 32 hours in fermention medium, 37 ℃ of condition bottom fermentations 20 hours, stirred fermention medium in the fermenting process, whenever stirred once at a distance from 6 hours.After the fermentation ends, under the vacuum tightness of 0.1Mpa, temperature 40-45 ℃, be dried to moisture content≤8%, then the product after the packing.Through detecting, the protein contnt of direct seeding compression nutrition pot raw material product is 24-28%, and expansion rate of water absorption is 450-650%.
Embodiment 9 direct seeding compression nutrition pot raw materials are to the influence of cotton effect of increasing production
Adopt plot experiment, soil is black earth, middle fertility, and physical features is smooth; Zone leader 10m, wide 5m, every interval is at a distance from 0.5m; Establish nutrition bowl seedling transplanting contrast and two processing of direct seeding compression nutrition pot in every district, repeat for totally 3 times, all take district's group arrangement at random between repetition and the processing.The nutrition bowl seedling transplanting technology is with reference to Xu Xunyuan, and cotton nutrition is grown seedlings and cultivated technology in morning in full strong sprout together, Jiangxi cotton, the 29th the 2nd phase of volume of April in 2007.The direct seeding compression nutrition pot technology adopts the direct seeding compression nutrition pot raw material of embodiment 5 preparations.
Select cotton No. 3 seeds with a collection of section for use, the field management condition is identical.Nutrition bowl seedling transplanting control group and direct seeding compression nutrition pot experimental group are sowed simultaneously, and after seedling grew, the nutrition bowl seedling transplanting control group was transplanted seedling to plot experiment Tanaka; Continuous observation 25 strains; Investigation plant height, individual plant fruit branch number, one-tenth bell number begin the branch sub-district from blow-of-cottons, pick flowers by stages; Subregion dries, and (4 flowers) bell of finally measuring in the compound sample is heavy.
Test-results and analysis:
1) table 1 is seen in two kinds of testing program cotton plant height result contrasts
Two kinds of testing program cottons of table 1 plant height
Test-results by table 1 can be found out, adopts its plant height MV of direct seeding compression nutrition pot plant cotton than the short 1.3cm of nutrition bowl seedling transplanting control group MV, its better effects if.
2) two kinds of testing program cotton individual plant fruit branches are counted result's contrast and are seen table 2
Two kinds of testing program cottons of table 2 individual plant fruit branch number
Test-results by table 2 can be found out, adopts its individual plant fruit branch of direct seeding compression nutrition pot plant cotton to count MV and Duos 0.8 than nutrition bowl seedling transplanting control group MV, more helps the cotton result.
3) two kinds of testing program cottons become bells to count result's contrast to see table 3
Two kinds of testing program cottons of table 3 become the bell number
Test-results by table 3 can be found out, adopts its cotton of direct seeding compression nutrition pot plant cotton to become bell to count MV and Duos 2.4 than nutrition bowl seedling transplanting control group MV, can improve output of cotton.
4) table 4 is seen in two kinds of heavy result's contrasts of testing program cotton bell
Two kinds of testing program cottons of table 4 bell is heavy
Test-results by table 4 can be found out, adopts the heavy MV of its cotton bell of direct seeding compression nutrition pot plant cotton than the high 0.38g of nutrition bowl seedling transplanting control group MV, can improve output of cotton.
The related tangerine bar of embodiment 10 this patents can be mixed with raw materials such as wood chip, peat soil, animal excrement, can improve the performance of compression nutrition bowl, therefore, relates to being mixed of these several types of raw materials and all belongs to the scope of this patent.
To sum up test-results is said; Adopt the direct seeding compression nutrition pot plant cotton to compare with nutrition bowl seedling transplanting; Can improve the output of cotton seedling quality and cotton; Can significantly reduce the labour intensity of cotton planting again, realize the mechanized operation of cotton planting better, have beneficial technical effects and remarkable economic efficiency.
Claims (4)
1. the preparation method of a direct seeding compression nutrition pot raw material is characterized in that: in turn include the following steps:
1) in fermention medium, insert the mould composite fungus agent of 8-12%, 30 ℃ of condition bottom fermentations 36-48 hour stir fermention medium in the fermenting process, and earlier fermentation whenever stirred once at a distance from 8 hours, fermented after 24 hours, whenever stirred once at a distance from 4 hours;
2) in fermention medium, insert the yeast composite fungus agent of 4-6%, 28 ℃ of condition bottom fermentations 16-24 hour stir fermention medium in the fermenting process, whenever stirred once at a distance from 4 hours;
3) in fermention medium, insert the vinelandii composite fungus agent of 6-10%,, stir fermention medium in the fermenting process, whenever stirred once at a distance from 3 hours 32 ℃ of condition bottom fermentations 24-36 hour;
4) in fermention medium, insert the bacillus subtilis microbial agent of 4-6%,, stir fermention medium in the fermenting process, whenever stirred once at a distance from 6 hours 37 ℃ of condition bottom fermentations 16-24 hour; After the fermentation ends, under the vacuum tightness of 0.1MPa, temperature 40-45 ℃, be dried to Shui Fen ≦ 8%;
By weight, said fermention medium is made up of the water of stalk 28%, ammonium sulfate 3%, urea 0.5%, potassium primary phosphate 1%, sal epsom 0.3%, calcium chloride 0.2% and surplus;
The activeconstituents of said mould composite fungus agent is made up of black mold, aspergillus oryzae, viride, geotrichum candidum;
The activeconstituents of said yeast composite fungus agent is made up of cereuisiae fermentum, candida tropicalis, Candida utilis;
The activeconstituents of said vinelandii composite fungus agent is made up of Bacillus foecalis alkaligenes and blown-ball Azotobacter.
2. the preparation method of direct seeding compression nutrition pot raw material as claimed in claim 1 is characterized in that: said crushed stalk is to the fineness of crossing the 30-60 mesh sieve.
3. the preparation method of direct seeding compression nutrition pot raw material as claimed in claim 1; It is characterized in that: the preparation process of said mould composite fungus agent is: in PDA solid slant culture base, be inoculated into respectively in the PDA liquid nutrient medium through twice activatory black mold, aspergillus oryzae, viride, geotrichum candidum bacterial classification; At 30 ℃; Cultivate under the rotating speed 180rpm condition, make spore count reach 2.5-6.0 * 10
8Individual/mL promptly gets seed liquor, by the inoculum size of 5-10% said seed liquor is inoculated into the substratum proportion of composing to be: stalk 90%, wheat bran 6%, (NH
4)
2SO
42%, urea 1%, KH
2PO
40.6%, MgSO
40.2%, CaCl
20.2%, moisture content is in the solid-state fermentation culture medium of total solids content 60-70%, and at 30 ℃, every at a distance from the condition bottom fermentation that turned over Qu Yici in 2-3 hour, tunning is vacuum-drying to moisture≤10% under 40 ℃ of conditions, and spore content 2.5-4 * 10
9Individual/gram, be the single strain preparation; With said black mold, aspergillus oryzae, viride, geotrichum candidum single strain preparation by weight the ratio uniform mixing of 3:2:3:1 promptly get the mould composite fungus agent.
4. the preparation method of direct seeding compression nutrition pot raw material as claimed in claim 1; It is characterized in that: the preparation process of said vinelandii composite fungus agent is: in the solid slant culture base of Asbby nitrogen-free agar, be inoculated into respectively in the liquid nutrient medium that said Asbby nitrogen-free agar is housed through twice activatory Bacillus foecalis alkaligenes, blown-ball Azotobacter; At 32 ℃; Cultivate under the rotating speed 180rpm condition, make cell count reach 5-7 * 10
8Individual/mL promptly gets seed liquor, by the inoculum size of 6-8% said seed liquor is inoculated in the Asbby nitrogen-free agar, and at 32 ℃, rotating speed 180rpm is cultured to cell count 3-5.5 * 10 under the air flow 0.5-1v/vmin condition
9Individual/mL, be the single strain preparation; With said Bacillus foecalis alkaligenes and blown-ball Azotobacter single strain preparation by volume the ratio uniform mixing of 1:1 promptly get the vinelandii composite fungus agent; The prescription of described Asbby nitrogen-free agar is: N.F,USP MANNITOL 10g, lime carbonate 5g, potassium primary phosphate 0.2g, sal epsom 0.2g, sodium-chlor 0.2g, calcium sulfate 0.1g, zero(ppm) water 1000ml, pH6.8-7.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102716126A CN101928161B (en) | 2010-09-03 | 2010-09-03 | Method for preparing direct seeding compression nutrition pot raw material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102716126A CN101928161B (en) | 2010-09-03 | 2010-09-03 | Method for preparing direct seeding compression nutrition pot raw material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101928161A CN101928161A (en) | 2010-12-29 |
| CN101928161B true CN101928161B (en) | 2012-11-14 |
Family
ID=43367649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102716126A Expired - Fee Related CN101928161B (en) | 2010-09-03 | 2010-09-03 | Method for preparing direct seeding compression nutrition pot raw material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101928161B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102276301B (en) * | 2011-06-02 | 2013-05-22 | 武汉金禾科技发展有限公司 | Straw decomposing agent and production method thereof |
| CN103026920A (en) * | 2012-12-29 | 2013-04-10 | 山东坤基沃业生物科技股份有限公司 | Fully degradable seedling-growing integrated nutritional bowl and production method thereof |
| CN103518560A (en) * | 2013-04-17 | 2014-01-22 | 武志学 | Ecological environment-friendly degradation seedling growing nutrition pot |
| CN103348881B (en) * | 2013-07-09 | 2015-03-11 | 德宏后谷咖啡有限公司 | Coffee grounds seedling raising matrix nutrition pot and manufacturing method thereof |
| CN104067884A (en) * | 2014-07-18 | 2014-10-01 | 扬州大学 | Rape potted-seedling cultivation method |
| CN107242045B (en) * | 2017-06-08 | 2021-01-01 | 内蒙古农业大学 | Degradable seedling raising pot with plant growth promoting effect and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618772A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | High efficiency biological organic fertilizer and its production technology |
| CN1965638A (en) * | 2005-11-15 | 2007-05-23 | 方立成 | Compact type substrate seedling nursery culture pan |
| CN101712566A (en) * | 2009-11-10 | 2010-05-26 | 陕西省科学院酶工程研究所 | Preparation method of soil moisturizing bio-fertilizer |
-
2010
- 2010-09-03 CN CN2010102716126A patent/CN101928161B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618772A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | High efficiency biological organic fertilizer and its production technology |
| CN1965638A (en) * | 2005-11-15 | 2007-05-23 | 方立成 | Compact type substrate seedling nursery culture pan |
| CN101712566A (en) * | 2009-11-10 | 2010-05-26 | 陕西省科学院酶工程研究所 | Preparation method of soil moisturizing bio-fertilizer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101928161A (en) | 2010-12-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2479253B1 (en) | Antagonistic bacteria for preventing and treating panama wilt disease of continuously planted banana and microorganism organic fertilizer thereof | |
| CN101659933B (en) | Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof | |
| CN101691549B (en) | Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof | |
| CN101870608B (en) | Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof | |
| CN101941851A (en) | Technology and process for preparing biochemical humic acid by using kitchen waste | |
| CN101165008B (en) | Vegetable residual strain microorganism-earthworm multiple step inoculation conversion method | |
| CN103966150A (en) | Crop straw decomposing inoculant and using method and effect thereof | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN103382134A (en) | Fermentation method of Agaricus bisporus (Lange) Sing cultivation matrix | |
| CN104788244A (en) | Organic fertilizer and preparation method thereof | |
| CN103382139A (en) | Agaricus bisporus (Lange) Sing culture medium | |
| CN104016745B (en) | microbial organic fertilizer and preparation method thereof | |
| CN101928161B (en) | Method for preparing direct seeding compression nutrition pot raw material | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
| CN105248233A (en) | Method of inoculating tea-oil tree seedlings with AM fungi and high-yield planting method for tea-oil trees | |
| CN107445694A (en) | A kind of composite bio-chemical preparation and its applied in straw-returning | |
| CN105481486A (en) | Method for producing trichoderma bio-organic fertilizer from straw and filtrated mud and obtained product | |
| CN110066748A (en) | A kind of complex microorganism and microbial inoculum and its application comprising the complex microorganism | |
| CN101659931B (en) | Antagonistic bacteria preventing and removing continuous cropping cucumber rhizoctonia rot and microbial organic fertilizer thereof | |
| CN103642782A (en) | Method for preparing straw-decomposing inoculant capable of inhibiting soil-borne diseases | |
| CN106396862A (en) | Disease controlling microbial fertilizer special for tuberous crops and preparation method thereof | |
| CN104844285A (en) | Method for preparing bio-organic fertilizer for improving immunity of cherry tomatoes | |
| CN110591937B (en) | Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, method and application | |
| CN106348888B (en) | A method of trichoderma as biological organic fertilizer is produced using shallot Folium Allii fistulosi waste |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: CROP INSTITUTE, HUNAN ACADEMY OF AGRICULTURAL SCIE Effective date: 20120807 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20120807 Address after: 430068 Wuchang, Hubei Province, South Lake, Li Jia Dun special No. 1 Applicant after: Hubei Industry University Co-applicant after: Crop Institute, Hunan Academy of Agricultural Sciences Address before: 430068 Wuchang, Hubei Province, South Lake, Li Jia Dun special No. 1 Applicant before: Hubei Industry University |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121114 Termination date: 20130903 |