CN105248233A - Method of inoculating tea-oil tree seedlings with AM fungi and high-yield planting method for tea-oil trees - Google Patents

Method of inoculating tea-oil tree seedlings with AM fungi and high-yield planting method for tea-oil trees Download PDF

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CN105248233A
CN105248233A CN201510817353.5A CN201510817353A CN105248233A CN 105248233 A CN105248233 A CN 105248233A CN 201510817353 A CN201510817353 A CN 201510817353A CN 105248233 A CN105248233 A CN 105248233A
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oil tea
oil
tea
bacterium
inoculation
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CN105248233B (en
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全宏靖
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Abstract

The invention provides a method of inoculating tea-oil tree seedlings with AM fungi and a high-yield planting method for tea-oil trees. The method of inoculating the tea-oil tree seedlings with the AM fungi comprises the steps that root segments where the AM fungi are parasitic of leguminous plants as well as spore soil on the root segments are dug, smashed, sown beside roots of the tea-oil tree seedlings, earthed up and cultivated for four months or more to complete inoculation. By the adoption of the method, the tea-oil tree seedlings can be successfully inoculated with roots of the AM fungi, and the inoculation is lifelong and accompanies with growth of the tea-oil trees all the time; moreover, the method has a high inoculation rate of the AM fungi and provides a new way for high-yield planting of the tea-oil trees; the tea-oil trees are long in growth cycle, and the purpose of continuous high yield cannot be achieved unless soil fertility is maintained; in the whole production process of oil tea, wormcast and earthworms are selected and used as special fertilizer for a tea garden, which serves as a reasonable agriculture measure in oil tea production.

Description

The method of oil tea seedling inoculation AM bacterium and the high-yield planting method of oil tea
Technical field
The present invention relates to crop genetics field, in particular to the method for oil tea seedling inoculation AM bacterium and the high-yield planting method of oil tea.
Background technology
Tea oil tree is one of large woody oleiferous plants in the world four, and the high mountain of its growth in southern china subtropical zone and hilly country, be the senior oil plant of the distinctive a kind of pure natural of China, be a kind of evergreen, broad-leaved, long-lived ecological economy seeds, once plant, be benefited for many years.The seed of oil tea can extract oil (tea oil) edible, therefore named.Tea oil is a kind of green non-pollution product, and its unsaturated fatty acid content reaches more than 90%, is better than olive oil, is the senior edible vegetable oil of a kind of health type, and food and agricultural organization of united state emphasis is promoted.Its look clear taste is fragrant, nutritious, is rich in the active substance very useful to human body, as vitamin A, D, K, E, squalene, and the functional components such as Tea Polyphenols and beta carotene, tea oil is not containing aflatoxin, and storage tolerance is high-quality edible oil; Tea oil also can be used as lubricating oil, antirust oil for industry.Tea cake is agricultural chemicals, is again fertilizer, is also the raw material of industry, and the tea oil by-product that aqueous enzymatic method refines can make nuisanceless detergent liquid, protection water environment, capable of using as feed, avoids using antibiotic to enter cultivation industry.Visible, camellia oleiferaindustry chain length, comprehensive utilization value is high, oil tea collection ecological benefits, economic benefit and social benefit are, for the national grain and oil safety of maintenance, advance Integrated Development of The Mountainous Region, promote that peasant employment increases income, improve people ' s health situation, accelerate territory greening process and all have a very important role.But current cultivation method output is general lower, seriously constrains the extensive use of oil tea.
AM (group planting mycorhiza) bacterium can promote as the absorption to nutrient, improves the resistance of crop to low temperature, arid, the adverse circumstance such as saline and alkaline, and reduces the infection of soil-borne disease and nematode, thus raising output, oil recovery enhancement, therefore, receives the concern of people day by day.Strengthen and earthworm and wormcast are introduced the oil tea ecosystem, performance earthworm and wormcast are contributed the growth of oil tea.
Also AM mycorhiza is not successfully inoculated into the relevant report of oil tea seedling at present.Also earthworm and wormcast are not introduced the report of the oil tea ecosystem at present.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of oil tea seedling to inoculate the method for AM bacterium, and AM mycorhiza can successfully be seeded on oil tea seedling by the method, and this inoculation is lifelong, and all the time with the growth of oil tea, and this inoculation method also has higher AM bacterium rate of vaccination.
The second object of the present invention is a kind of high-yield planting method providing oil tea, this implantation methods is owing to being vaccinated with AM bacterium, and in omnidistance production process, introduce wormcast and the use of this committed step of earthworm on oil tea, therefore the disease resistance power of tea oil tree is strong, high to the absorption ratio of nutrient component, output improves thereupon.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
The method of oil tea seedling inoculation AM bacterium, comprises the following steps:
Take leguminous plant parasitism and have the root segment of AM bacterium and related spore soil thereof, smash to pieces, be seeded in the root limit of oil tea seedling, blinding, cultivate more than four months, complete inoculation.
First, above-mentioned inoculation method is different from the method that other crop in conventional art inoculates AM bacterium.
Traditional method is directly seeded in target crop by pure AM bacterium, this method is selective to crop species, only has the AM bacterium of the successful seeded with pure of crop ability of the part kinds such as pulse family, its main cause is: AM fungi is selective, parasitic survival rate on variety classes host is different, therefore the inoculation rate on variety classes crop is different, and production effect is also just different.
And the present invention is in order to avoid above problem, it is root limit AM bacterium and the high host-leguminous plant of its survival rate being together seeded in oil tea seedling, the AM bacterium of using high viability on leguminous plant root segment infects oil tea shoot root portion, improve the infection rate of primary stage of inoculation, be mixed into the soil (i.e. spore soil) containing a large amount of AM spore simultaneously, in seeded process, breed a large amount of AM bacterium continuously, rhizobium leguminosarum, two inoculations like this, improve the infection rate of inoculation later stage AM mycorrhizal fungi.As can be seen here, the present invention is by maintaining continual high infection rate, being successfully seeded on oil tea shoot root by AM bacterium.
Secondly, physiological property due to leguminous plant itself determines that it carries rhizobium, and when therefore infecting oil tea seedling with it, the nitrogenous fertilizer that rhizobium can also be made to produce is that oil tea is used, obtaining the effect of killing two birds with one stone, providing another advantage for improving tea-oil tree yield.
In addition, under normal circumstances, the root segment of AM bacterium and the sowing of related spore soil thereof is had by parasitism to cultivate after oil tea seedling after four months, inoculate successfully, can row field planting again, if certainly obtain higher infection rate, also can extend planting time again, such as five months or Second Year are transplanted later again.
The plant that leguminous plant root segment used in above-mentioned inoculation method can adopt common cultivation method to cultivate is because fabaceous root itself is also spontaneous AM bacterium, just low with the postvaccinal infection rate of this plant.Also following special cultivation method can be adopted, the content with enrichment fabaceous root AM bacterium:
Cultivation one: preferably, described leguminous plant adopts following methods cultivation:
There is the leguminous plant root segment of root nodule broken by what preserve in advance, then itself and fabaceous seed of the same race are soaked in water jointly, until seed breaks chest, sow seed afterwards, results plant; Wherein, the leguminous plant root segment of root nodule is had to be fresh root segment or the root segment through drying described in.
Can make the AM bacterium that fabaceous seed infection is a large amount of so in advance, then constantly breed in the course of cultivation, then superpose seed and take root from the AM bacterium that bears, the shoot root portion AM bacterial content now cultivated is very high, enough meets inoculation demand.
The root segment of Rhizobium Inoculation both can adopt fresh, and also can adopt outmoded, both differences are: the root segment that upper one year gathers is convenient to preserve, and carries, and introduces the clover rhizobia of different regions, enriches root nodule germ plasm resource.Fresh gathers materials on the spot, and inoculation efficiency is high.
In addition, in above seeding process, note following item: bed surface after planting will be kept moistening, cause the seed of sprouting to bounce back and dead so as not to lack of water, sowing just unsuccessfully.For the cycle of cultivation, as long as leguminous plant growth situation is good, namely can be used to inoculate oil tea.Certainly, in order to can management be continued, analytical technology also can be adopted to detect the infection rate of leguminous plant root AM bacterium and rhizobium, as a reference.
Cultivation two: preferably, described leguminous plant adopts following methods cultivation: in the soil of fabaceous seedling described in the described fabaceous seed of sowing or field planting, apply AM bacterium, continue cultivation; And more than in cultivation, base fertilizer used contains phosphate fertilizer.
The method directly infects leguminous plant root with AM microbial inoculum, and phosphate fertilizer of will arranging in pairs or groups, phosphate fertilizer is conducive to infecting of AM, improves infection rate.Equally, for the cycle of cultivation, as long as leguminous plant growth situation is good, namely can be used to inoculate oil tea.
Preferably, base fertilizer used in this cultivation is also containing wormcast and/or charcoal powder.Wormcast, as fertilizer, is conducive to the breeding of AM.The carbonic acid gas of discharging into the atmosphere when charcoal powder Absorbable rod organic substance rots, and help plant effectively to store carbonic acid gas needed for its photosynthesis; And charcoal powder is loose porous, in alkalescent, it is the desirable family of microorganism, make AM mycelia can enter charcoal inside and produce spore, and underground imbedded by charcoal, can rehabilitating soil, culture fertility, namely charcoal is all favourable to plant, soil and microorganism, comprehensively can improve infection rate and the proliferative speed of AM.Wherein, during the combination of wormcast, charcoal powder and phosphate fertilizer, be good with 12:7:1 weight ratio.
Preferably, in the described fabaceous process of cultivation, the humus soil from oil tea root limit is applied.So both can provide abundant organic matter for plant growth and microbial reproduction, the growing environment of rhizobium, AM bacterium adaptation oil tea can be made again, for later stage two kinds and oil tea symbiotic co-existence provide advantage.
Preferably, described leguminous plant is clover.The AM bacterial classification class of clover root parasitism is many, and the outer mycelia of root of AM fungi can provide the phosphorus of 70% and the nitrogen of 30% to plant, sets up oil tea-Mycorrhizal Symbiosis system.Therefore stronger by its disease resistance power inoculating the kind that oil tea obtains, higher to the absorption ratio of nutrient component, output is higher.
Preferably, when cultivating oil tea seedling in described steps A, apply the base fertilizer mainly consisted of the following composition: by weight, 2.5-3.5 part wormcast, 4-6 part soil, 1.5-2.5 part charcoal powder, 0.01 part of phosphate fertilizer.The fertilizer of this ratio can promote that AM's infects speed and appeal, shortens transfer period, improves infection rate.
A high-yield planting method for oil tea, comprises the following steps:
The method of oil tea seedling inoculation AM bacterium mentioned above is utilized to cultivate oil tea seedling;
By the field planting of described oil tea seedling in field, carry out daily management, complete plantation, gather.
With mentioned above, owing to being vaccinated with AM bacterium in this implantation methods, therefore the disease resistance power of tea oil tree is strong, and high to the absorption ratio of nutrient component, output improves thereupon.Through investigating, compared with the oil tea seedling of common variety, implantation methods tea oil of the present invention annual production can improve about 30%, and every mu of oil produces 50-100 kilogram.
Preferably, base fertilizer used during described field planting is: wormcast, charcoal powder, and both weight ratios are 3.5-4.5:1.This ratio more meets the growth characteristics of oil tea, balances each other with its nutrient absorption property.In addition, adopt this base fertilizer, adaptable to weather of tea oil tree, except freezing season, plantation tea oil tree of can going up a hill the whole year.Certainly, above base fertilizer also can regularly apply at later stages, and fertilizer application frequency can see the daily record of oil tea whole year production, and also can disposable the wormcast of a year all be executed in oil tea ground autumn, oil tea plant next year opposite be executed, to promote that oil tea root system develops.
Preferably, described daily management adopts one or more in following six kinds of modes:
Mode one: be soaked in water and soak nettle cauline leaf more than 10 days, filters and obtains nettle slurry; Described nettle slurry is splashed on oil tea ground or tea oil tree; Nettle slurry had both increased soil fertilizer, Control pests again.Particularly use with the dusk morning of high temperature season, successful.
Mode two: breed earthworm in oil tea ground, and described breeding fertilizer used is preferably: farmyard manure; Earthworm is exactly free loosening the soil, fertilising workman.Every earthworm is all the combination of a marvellous loosenning tiller and fertilizer processing factory.Earthworm is expanded numerous on the spot, and earthworm connects earthworm after how breeding and wormcast is placed in oil tea green manure ground.Soil great majority because of kind of oil tea be engineering wholely, growing surface is mostly the new soil of non-slaking, and nutrient content is low, in the new soil that these do not become thoroughly decomposed, repairs its ecosystem function with earthworm reacting ring.Because there being earthworm, therefore dead soil becomes fertile.Earthworm and wormcast are introduced the oil tea ecosystem, makes the oil tea ecosystem more stable.
Mode three: in inner room, oil tea ground every plantation rape, soya bean, clover, perennial ryegrass, winged bean, one or more in hybrid Chinese pennisetum, and regularly these green manure crops are cut blue or green earth backing; These are very environmental protection, the organic matter of low cost, humus, can optimize the ecosphere of Growth of Camellia oleifera.In addition, can also to dress seed green manuring with wormcast when cultivating above crop.
Mode four: to oil tea apply ferment soya bean slurry;
Soya bean slurry fertilizer is mainly for oil tea provides available nutrient, in oil tea rudiment, bloom, open up leaf season, the season of bearing fruit, oil tea nutrient consumption is large, need liquid manure more, pulling an oar after soybean soaking, to being watered after fermentation, splashing after becoming thoroughly decomposed in oil tea ground, to high potassium, middle nitrogen and low-phosphorous fertilizer that fruit transport carbohydrate needs, increase fruit fat content.
Mode five: apply EM bacterium to oil tea;
EM bacterium comparatively fast and stably can occupy the ecologic niche in soil, forms the advantage group of useful microbial bacteria, thus controls the breeding of pathogenic microorganism and the invasion and attack to crop.
Mode six: put Pollinating Insect in a suitable place to breed in oil tea ground, helps pollination of oil tea tree.
In addition, Na Shi can also be sprayed during Growth of Camellia oleifera and trimly send out, improve the photosynthesis of oil tea.
Compared with prior art, beneficial effect of the present invention is:
(1) successful incubation goes out a kind of oil tea seedling kind of AM bacterium height infection rate.
(2) cultivation method of a kind of AM bacterium of increasing is developed.
(3) a kind of Fertilizer Combination with microecosystem circulating ring feature is worked out: wormcast, charcoal powder, phosphate fertilizer.
(4) cultivation step such as water and fertilizer management, weeding deinsectization, pollination in oil tea plantation is optimized.
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturer suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
Embodiment 1
Oil tea is planted:
Nursery: have the clover root segment of root nodule broken fresh, then itself and clover seed are soaked in water, jointly until seed breaks chest, sow seed afterwards, simultaneously Wild Camellia oleifera Abel humus soil wormcast mix, be sowed at clover seed limit, water, until take its root segment and related spore soil thereof during seedling, smash to pieces, be seeded in the root limit of oil tea seedling, blinding, cultivate four months, complete inoculation, results oil tea seedling.
Wholely, field planting: select mountain region, apply fertilizer to the subsoil, the oil tea seedling cultivated is transplanted in mountain region by regulation seeding row spacing.
Management: regularly fertilising, weeding deinsectization.
Gather: seed of gathering, oil expression.
Embodiment 2
Oil tea is planted:
Nursery: in clover in first year season floriferous, dig clover root, will protect the root of root nodule especially, cleans, puts shady and cool ventilation place and dry in the shade.By the time clover sowing season, the grass roots originally having clover root nodule, shred with cutter, soak with cold boiling water, then clover seed is soaked wherein, be dipped to seed and break chest, sow seed afterwards, simultaneously Wild Camellia oleifera Abel humus soil wormcast mix, be sowed at clover seed limit, water, until take its root segment and related spore soil thereof during seedling, smash to pieces, be seeded in the root limit of oil tea seedling, blinding, cultivate four months, complete inoculation, results oil tea seedling.
Wholely, field planting: select mountain region, apply fertilizer to the subsoil, the oil tea seedling cultivated is transplanted in mountain region by regulation seeding row spacing.
Management: regularly fertilising, weeding deinsectization.
Gather: seed of gathering, oil expression.
Embodiment 3
Oil tea is planted:
Camellia seedling: choose ground, applies the base fertilizer being mixed with farmyard manure (castor-oil plant cake fertilizer, tung oil tree cake fertilizer) and phosphate fertilizer (every kilogram of soil joins 40 milligrams of phosphate fertilizer) wherein, and sowing clover seed, applies AM bacterium simultaneously, continue cultivation in soil; Until take its root segment and related spore soil thereof during seedling, smash to pieces, be seeded in the root limit of oil tea seedling, blinding, cultivate four months, complete inoculation, results oil tea seedling.
Wholely, field planting: select mountain region, apply fertilizer to the subsoil, the oil tea seedling cultivated is transplanted in mountain region by regulation seeding row spacing.
Management: regularly fertilising, weeding deinsectization.
Gather: seed of gathering, oil expression.
Embodiment 4
Oil tea is planted:
Nursery: select the trefoil field of plantation, apply base fertilizer (wormcast, vermiculite, phosphorus, molydbenum fertilizer, weight ratio is 3.5:5.9:0.5:0.01) wherein,
Sowing clover seed, applies AM bacterium simultaneously, continues cultivation in soil; Simultaneously Wild Camellia oleifera Abel humus soil wormcast mix, be sowed at clover seed limit.Until take its root segment and related spore soil thereof during clover seedling, smash to pieces.Select the field of cultivating oil tea seedling, apply base fertilizer (wormcast, soil, charcoal powder, phosphate fertilizer), plantation oil tea seedling, is seeded in the root limit of oil tea seedling, blinding, cultivates four months, complete inoculation by the clover root segment smashed to pieces, results oil tea seedling.
Wholely, field planting: select mountain region, apply fertilizer to the subsoil (wormcast, charcoal powder, both weight ratios are 4:1), transplanted in mountain region by the oil tea seedling cultivated by regulation seeding row spacing.
Management: regularly fertilising, weeding deinsectization.
Gather: seed of gathering, oil expression.
Embodiment 5
Oil tea is planted:
Nursery: select the trefoil field of plantation, apply base fertilizer (wormcast, the cellular pellet coal dust burnt and phosphate fertilizer are with the combination of 6:13:1 weight ratio) wherein, sowing clover seed, applies AM bacterium simultaneously, continues cultivation in soil; Until take its root segment and related spore soil thereof during seedling, smash to pieces.Select the field of cultivating oil tea seedling, apply base fertilizer (wormcast, soil, charcoal powder, phosphate fertilizer form with the weight ratio of 3.5:4:2.5:0.01), plantation oil tea seedling, the clover root segment smashed to pieces is seeded in the root limit of oil tea seedling, blinding, cultivate four months, complete inoculation, results oil tea seedling.
Wholely, field planting: select mountain region, apply fertilizer to the subsoil (wormcast, charcoal powder, both weight ratios are 3.5:1), transplanted in mountain region by the oil tea seedling cultivated by regulation seeding row spacing.
Management:
Be soaked in water and soak nettle cauline leaf more than 10 days, filter and obtain nettle slurry; Described nettle slurry is splashed on oil tea ground or tea oil tree;
In oil tea ground, breed earthworm, and described breeding fertilizer used is: farmyard manure;
In inner room, oil tea ground every plantation rape, soya bean, and regularly these subsidiary crops are cut blue or green earth backing;
To oil tea apply ferment soya bean slurry;
EM bacterium is applied to oil tea;
Pollinating Insect is put in a suitable place to breed in oil tea ground.
Gather: seed of gathering, oil expression.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.

Claims (10)

1. the method for oil tea seedling inoculation AM bacterium, is characterized in that, comprise the following steps:
Take leguminous plant parasitism and have the root segment of AM bacterium and related spore soil thereof, smash to pieces, be seeded in the root limit of oil tea seedling, blinding, cultivate more than four months, complete inoculation.
2. the method for oil tea seedling inoculation AM bacterium according to claim 1, it is characterized in that, described leguminous plant adopts following methods cultivation:
There is the leguminous plant root segment of root nodule broken by what preserve in advance, then itself and fabaceous seed of the same race are soaked in water jointly, until seed breaks chest, sow seed afterwards, results plant; Wherein, the leguminous plant root segment of root nodule is had to be fresh root segment or the root segment through drying described in.
3. the method for oil tea seedling inoculation AM bacterium according to claim 1, it is characterized in that, described leguminous plant adopts following methods cultivation: in the soil of cultivation, apply AM bacterium and the base fertilizer containing phosphate fertilizer.
4. the method for oil tea seedling inoculation AM bacterium according to claim 3, it is characterized in that, described base fertilizer is also containing wormcast and/or charcoal powder.
5. the method for the oil tea seedling inoculation AM bacterium according to Claims 2 or 3, is characterized in that, in the described fabaceous process of cultivation, apply the humus soil from oil tea root limit.
6. the method for oil tea seedling inoculation AM bacterium according to claim 1, it is characterized in that, described leguminous plant is clover.
7. the method for oil tea seedling inoculation AM bacterium according to claim 1, is characterized in that, when cultivating oil tea seedling in described steps A, apply the base fertilizer mainly consisted of the following composition: by weight, 2.5-3.5 part wormcast, 4-6 part soil, 1.5-2.5 part charcoal powder, 0.01 part of phosphate fertilizer.
8. the high-yield planting method of oil tea, is characterized in that, comprise the following steps:
The method of the oil tea seedling inoculation AM bacterium described in any one of claim 1-7 is utilized to cultivate oil tea seedling;
By the field planting of described oil tea seedling in field, carry out daily management, complete plantation, gather.
9. the high-yield planting method of oil tea according to claim 8, is characterized in that, base fertilizer used during described field planting is: wormcast, charcoal powder, and both weight ratios are 3.5-4.5:1.
10. the high-yield planting method of oil tea according to claim 8, is characterized in that, described daily management adopt in following six kinds of modes one or more:
Mode one: be soaked in water and soak nettle cauline leaf more than 10 days, filters and obtains nettle slurry; Described nettle slurry is splashed on oil tea ground or tea oil tree;
Mode two: breed earthworm in oil tea ground, and described breeding fertilizer used is preferably: farmyard manure;
Mode three: in inner room, oil tea ground every plantation rape, soya bean, clover, perennial ryegrass, winged bean, one or more in hybrid Chinese pennisetum, and regularly these subsidiary crops are cut blue or green earth backing;
Mode four: to oil tea apply ferment soya bean slurry;
Mode five: apply EM bacterium to oil tea;
Mode six: put Pollinating Insect in a suitable place to breed in oil tea ground.
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CN105993459A (en) * 2016-05-20 2016-10-12 安徽省天瑞园生态农业科技有限公司 Planting method for loofah
CN108781958A (en) * 2018-04-27 2018-11-13 铜仁市万山区黔东优质油茶种植有限公司 A kind of oil tea implantation methods
CN109280670A (en) * 2017-07-21 2019-01-29 中国科学院上海生命科学研究院 Regulation fatty acid synthesis gene and the method for promoting leguminous plant Mycorrhizal Symbiosiss
CN109644705A (en) * 2017-10-11 2019-04-19 贵州省茶叶研究所 A kind of tea shoot Mycorrhizal cutting propagation new method
CN114375640A (en) * 2022-01-19 2022-04-22 贵州省生物研究所 Method for promoting growth of camellia oleifera seedlings by using dark-color endophytic fungi
CN115316182A (en) * 2022-08-18 2022-11-11 广西壮族自治区农业科学院 Cultivation method for applying drip irrigation tea trees by using soybean milk tail pulp

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