CN103980020A - Green biological organic fertilizer prepared from multifunctional rotting microbial inoculant and natural organic substance material - Google Patents
Green biological organic fertilizer prepared from multifunctional rotting microbial inoculant and natural organic substance material Download PDFInfo
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Abstract
The invention discloses a green biological organic fertilizer prepared from a multifunctional rotting microbial inoculant and a natural organic substance material. The fertilizer is prepared by the following steps: adding the multifunctional organic substance rotting microbial inoculant into the mixture of multiple natural organic substances, and sufficiently fermenting and rotting. The fertilizer is suitable for planting organic agricultural products. The rotting microbial inoculant comprises the following active components: Bacillus subtilis, Bacillus licheniformis, Sporotrichum thermophile, Aspergillus oryzae and Trichoderma viride; the natural organic substance material comprises lignite, ground phosphorite, bean pulp and wheat bran; and the weight ratio of the rotting microbial inoculant to the natural organic substance material is 1:100. In the fertilizer, the organic matter content is 50%, the total nutrient content is 9%, the amino acid content is 8%, and the effective viable count is 2*10<8>/g; the fertilizer is free from the hazards of heavy metals, disease ova and weed seeds; the fertilizer can restore the soil, fertilize the soil, enhance the disease tolerance, increase the yield and improve the quality; when the fertilizer is applied to planting cucumbers, the yield increase per mou is 7.47-26.93%, and the disease prevention effect is 51.1%; and when being applied to planting tomatoes, the fertilizer enhances the contents of total sugar, soluble solids and red pigment, and lowers the content of nitrates.
Description
Technical field
The invention belongs to the technical field that biofermentation technique fermentation natural organic matter material is produced green fertilizer, relate to the green bio fertilizer that the multi-functional decomposing microbial inoculum of a kind of use and natural organic matter material are produced.
Background technology
In the recent period, aspect food safety, there are many alarming problems in China, including agricultural-food, also there is a lot of problems, such as the excessive Practice for Pesticide Residue in Agricultural Products causing of spraying insecticide exceeds standard, the quality of agricultural product that excessive use chemical fertilizer causes declines, accumulation of mouthfeel variation and carcinogenic substance nitrite etc.Severe in the face of these but must solve problem time, by reducing or rationally ensureing that with agricultural chemicals and chemical fertilizer yield and quality is on the one hand, plant organic farm products as also seem very important of a kind of important solution.
The Organic farming mode of production is to utilize the Efficient Cycle of animal, plant, microorganism and 4 kinds of factor of productions of soil, do not break the mode of production of biological cycle chain, the principle of Organic farming is to produce under the closed circulation state of agriculture energy, and all processes all utilizes agricultural resource.A topmost difference of organic farm products and other agricultural-food is exactly that organic farm products absolute prohibition in process of manufacture uses the synthetic materials such as any agricultural chemicals, chemical fertilizer, hormone, and does not allow to use genetic engineering technique; Other agricultural-food allow these materials of limited use, and do not ban use of genetic engineering technique.
But, the plantation of the organic farm products nutrition that needs fertilizer the same as other agricultural-food, otherwise will be not long, also need disease and pest to prevent, otherwise will suffer disaster.Therefore,, in the situation that can not using chemical fertilizer and agricultural chemicals, select rational organic fertilizer and disease-resistant mode to seem particularly important.The organic fertilizer major part using at present in agricultural planting is produced with feces of livestock and poultry, these fertilizers contain a large amount of heavy metals, also carry some sex pheromones and worm's ovum, still from food safety aspect, all do not met the production requirement of Organic farming from organic farm products standard.At present, the fertilizer that can meet Organic farming plantation comprises natural phant fertilizer and microbial fertilizer (without genetically engineered), and the agricultural chemicals that can meet Organic farming plantation only has biological pesticide.
Summary of the invention
For above problem, special proposition the present invention.This invention is a kind of green bio fertilizer with natural nutrient, organic matter, disease resistance and active bio, this fertilizer is by adding a kind of multi-functional decomposing microbial inoculum in the multiple natural organic matter material mixing, produce and make through fully becoming thoroughly decomposed fermentation, be specially adapted to the plant development of organic farm products.
The invention discloses for achieving the above object following technology contents:
A kind of green bio fertilizer, is characterized in that it is to be made up of multi-functional organic material composting bacteria fermentation natural organic matter material, and wherein the ratio of weight and number of multi-functional organic material composting microbial inoculum and natural organic matter material is 1:100; Green bio fertilizer living bacteria count is 2 × 10
8individual/g; The parts by weight of described organic material composting microbial inoculum are as follows:
Subtilis 1～3 Bacillus licheniformis 0.5～2
Sporotrichum thermophile 1～2 aspergillus oryzae 0.5～1
Viride 0.5～3;
The parts by weight of described natural organic matter material are as follows: 5～15 parts, 40～60 parts, brown coal, 20～40 parts, ground phosphate rock, 5～15 parts of dregs of beans and wheat bran.
Wherein brown coal organic content >=45, ground phosphate rock phosphorus pentoxide content >=30%, dregs of beans total nitrogen content >=5%, wheat bran is fresh not snake.
The present invention further discloses the production method of green green bio fertilizer, it is characterized in that being undertaken by following step:
(1) raw material and formula: fully mix taking by weight brown coal, ground phosphate rock, dregs of beans and wheat bran mixer, wherein brown coal account for 40～60 parts; Ground phosphate rock accounts for 20～40 parts; Dregs of beans accounts for 5～15 parts; Wheat bran accounts for 5～15 parts; Brown coal organic content >=45%, ground phosphate rock phosphorus pentoxide content >=30%, dregs of beans total nitrogen content >=5%, wheat bran is fresh not snake;
(2) add water: the weight ratio that is 1:1 by water and mixing raw material ratio of weight and number adds water, control water content 50～60%;
(3) add multi-functional organic material composting microbial inoculum: the ratio taking ratio of weight and number as 1:100 adds function organic material composting microbial inoculum with mixing raw material by multi-functional organic material composting microbial inoculum;
(4) specification of banking up: will regulate water and add the organic bank of mixing of multi-functional organic material composting microbial inoculum to grow up trapezoidal, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, heap is long to be determined according to place, and every heap volume is no less than 5 sides;
(5) control of turning time: after the fermentation of banking up starts, if fermentation initial stage temperature does not rise, as inadequate in moisture content, adjust moisture to recommendation ratio 50～60%, as excessive in moisture content, must turn over every day and smash once, increase oxygen content in buttress, impel microorganism Fast Growth, until heap temperature rises.In the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once, in order to killing harmful pathogenic micro-organism and the worm's ovums such as excrement enterobacteria and roundworm egg.When heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once.Turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, in the time that temperature exceedes 70 DEG C, need turn over and smash immediately, eliminate the inhibition of high temperature to microorganism growth by cooling, thereby impel the activity of a large amount of mesophilic bacterias, accelerate the process of compost.
(6) turning requires: turn in buttress process and will accomplish tune, even, broken; Adjusting is exactly original top section in buttress to be transferred to earth's surface become underclad portion, and underclad portion is transferred to top layer and is become top section; Even is exactly that buttress Raw is mixed to also inhomogeneous part, again mixes work; Broken is exactly that the massive zymolysis thing of formation is during the fermentation carried out to fragmentation work;
(7) fermentation termination control: heap temperature is identical with external temperature, moisture is while being down to below 30%, outward appearance chocolate, odorless, has completed the whole fermentation production process of green organic fertilizer, and the whole cycle is generally 15～20 days.
(8) fermented quality control: pulverize after having fermented, sieve, detect, weigh and pack, green organic manure products production completes; Wherein organic requirement of green organic manure reaches 50%, N, P, K total nutrient 9%, contains abundant amino acid, and aminoacids content reaches 8%, living bacteria count > 2 × 10
8individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
Green bio fertilizer of the present invention relates to multi-functional organic material composting microbial inoculum and natural organic matter material, both are indispensable, this fertilizer is in the mixture being combined by multiple natural organic matter material, to add after a kind of multi-functional organic material composting microbial inoculum, after abundant fermentation maturity, produce and obtain, be specially adapted to the plant development of organic farm products.
The present invention further discloses green bio fertilizer in the disease resistance that strengthens crop, reduces the injury of chemical fertilizer and agricultural chemicals, and raising output is improved crop quality and promoted the application that increases income aspect.
The multi-functional organic material composting microbial inoculum the present invention relates to, its activeconstituents comprises subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), sporotrichum thermophile (Sporotrichum thermophile Apinis), aspergillus oryzae (Aspergillus oryzae) and viride (Trichoderma viride).
The weight ratio of described subtilis, described Bacillus licheniformis, described sporotrichum thermophile, described aspergillus oryzae and described viride is 1～3:0.5～2:1～2:0.5～1:0.5～3.
It is the bacterial strain of ACCC 11089 that described subtilis specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.
It is the bacterial strain of ACCC 04249 that described Bacillus licheniformis specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.
It is the bacterial strain of ACCC 30346 that described sporotrichum thermophile specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.
It is the bacterial strain of ACCC 30155 that described aspergillus oryzae specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.
It is the bacterial strain of ACCC 30166 that described viride specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.
Multi-functional organic material composting microbial inoculum of the present invention integrates cellulose decomposition, protein decomposition, disease-resistant and bacterial manure several functions, add and mix after natural organic matter material, probiotics on the one hand decomposes the natural organic matter material that becomes thoroughly decomposed and forms the fertilizer of stable high nutrient, utilize organic materials to carry out self-reproduction on the one hand, in the fertilizer generating, make the fertilizer generating also there is disease-resistant and performance bacterial manure own disease resistance and bacterial manure function transplanting.
Green bio fertilizer of the present invention has various features, and first, raw materials for production are all made up of natural raw material, pollution-free; Secondly, natural organic matter and inorganic nutrients content are high, can meet the demand of organic grow nutrient; The 3rd, the sick worm's ovum carrying is considerably less, can reduce in a large number the generation of diseases and pests of agronomic crop; The 4th, there is a large amount of active beneficial microorganisms, can be disease-resistant, the conversion that can promote nutrient, can also improve Soil structure etc.; Finally, this fertilizer in conjunction with having manufactured a kind of three-in-one novel green fertilizer, had both been brought into play advantage separately by inorganic nutrients, organic nutrient and biophase, had overcome again shortcoming separately.
Green bio fertilizer of the present invention can promote soil remediation, and culture fertility strengthens the disease resistance of crop simultaneously, reduces the usage quantity of chemical fertilizer and agricultural chemicals, reduces and drops into, and improves output, improves crop quality, promotes to increase income.
Green bio fertilizer chocolate of the present invention, odorless, tasteless, easy to use.Organic matter reaches 50%, N, P, K total nutrient reaches 9%, contains abundant amino acid, and aminoacids content reaches more than 8%, living bacteria count > 2 × 10
8individual/g.Use this green organic fertilizer mu amount of increase in production 7.47～26.93%.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.Test materials used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.
embodiment 1:
the preparation of machine material decomposing microbial inoculum
Quantitative test in following examples, all arranges and repeats experiment, results averaged for three times.Following bacterial strain is all available from Chinese agriculture microbial strains preservation administrative center (Agricultural Culture Collection of China is called for short ACCC):
Subtilis (Bacillus subtilis): ACCC deposit number is 11089, is called for short ACCC11089;
Bacillus licheniformis (Bacillus licheniformis): ACCC deposit number is 04249, is called for short ACCC 04249;
Sporotrichum thermophile (Sporotrichum thermophile Apinis): ACCC deposit number is 30346, is called for short ACCC30346; Aspergillus oryzae (Aspergillus oryzae): ACCC deposit number is 30155, is called for short ACCC30155;
Viride (Trichoderma viride): ACCC deposit number is 30166, is called for short ACCC30166.
One, the preparation of substratum
1, potato, glucose agar medium (PDA)
200 grams of potatos removing skin, are cut into small pieces, and the 1000ml that adds water boils 30 minutes, elimination potato ball, filtrate is complemented to 1000ml, add 20 grams of glucose, 15 grams, agar, dissolves rear packing, 15 pounds of sterilizings 30 minutes, pH nature, does not add agar when obtaining liq substratum.
2, nutrition nutrient agar
Formed by peptone 5g, sodium-chlor 5g, extractum carnis 3g and distilled water 1000ml.PH7.0(solid medium adds agar 15-20g).
3, fungus solids fermention medium
Wheat bran 80g, dregs of beans 10g, (NH
4)
2sO
41.0g, KH
2pO
40.2g, MgSO
47H
200.05g, 80mL distilled water.
Two, the activation of bacterial classification
Subtilis and Bacillus licheniformis are seeded on nutrition nutrient agar substratum; Sporotrichum thermophile, aspergillus oryzae and viride are seeded on PDA substratum.
Subtilis, Bacillus licheniformis, sporotrichum thermophile, aspergillus oryzae and viride are carried out respectively to inclined-plane switching, access respective ramp substratum, activation culture 2-3 days.
Three, the mutual antagonistic experiment between bacterial strain
Subtilis, Bacillus licheniformis, sporotrichum thermophile, aspergillus oryzae and viride after activation are carried out to the mutual antagonistic experiment between bacterial strain, and combination of two, is divided into into 20 kinds of array modes, three repetitions of each combination.
Adopt steel ring method to carry out bacteriostatic test, bacterial strain is cultivated in corresponding substratum to 2-3 days (190r/min, 28 DEG C) and made fermented liquid.Getting the wherein fermented liquid 0.1mL of a kind of bacterium (A) is applied on PDA flat board, then on the solid medium of each flat board, put the steel ring of two prior sterilizings, in each steel ring, add again the fermented liquid of any bacterium (B) outside the A bacterium of 0.25mL, each processing in triplicate, taking the plate that only connects A bacterium as contrast.Cultivate and the antibacterial situation of routine observation for 28 DEG C.
The results are shown in Table 1.
Antagonistic effect between table 1 bacterial classification
| Subtilis | Bacillus licheniformis | Sporotrichum thermophile | Aspergillus oryzae | Viride | |
| Subtilis | - | - | - | - | |
| Bacillus licheniformis | - | - | - | - | |
| Sporotrichum thermophile | - | - | - | - | |
| Aspergillus oryzae | - | - | - | - | |
| Viride | - | - | - | - |
Note: "-" represents antagonism reaction negative, "+" represents antagonism reacting positive
Test-results shows unrestraint effect between subtilis, Bacillus licheniformis, sporotrichum thermophile, aspergillus oryzae and 5 kinds of bacterium of viride, and antagonism reaction is all negative.
Three, the preparation of organic material composting microbial inoculum (representing with composite fungus agent below)
1, the cultivation of bacteria culture and fermentation
Wherein 2 strains are bacterial isolateses, they are subtilis and Bacillus licheniformis, slant medium and shake-flask culture base are all nutrition nutrient agar, when preparation solid medium, add 15 grams of agar, seeding tank and fermentation tank culture medium are soya-bean milk, the starch of 6.5kg, the 1kg glucose of 1000kg water, 8.75kg soybean, CaCO
3100g, FeCl
310g, pH7.0,, cultivates 48 hours at 28 DEG C～30 DEG C after 30 minutes through 121 DEG C of real tank sterilizings, and in every ml fermented liquid, bacterium number reaches 6 × 10
8individual/g.Then being adsorbed on the vermiculite of 1 part normal temperature by the fermented liquid of 3 parts of weight ratios respectively dries and obtains subtilis and Bacillus licheniformis single strain microbial inoculum.
2, the cultivation of fungi bacterium and fermentation
Fungal bacterial strain comprises sporotrichum thermophile, aspergillus oryzae and viride 3 fungal strains.Inclined-plane and shake-flask culture base are potato, glucose agar medium, and slant medium adds agar.Liquid nutrient medium is sub-packed in 500ml triangular flask after sterilizing, accesses each fungi slant strains, shaking table speed 180r/min, cultivates 48 hours for 28 DEG C～30 DEG C, and when occurring in shaking flask that density is large, mycelium pellet is obviously not muddy.
Fungal bacterial strain seeding tank and fermentation cylinder for fermentation substratum are the cooking liquor of starch, 1kg glucose and the 2kg potato of soya-bean milk, the 6.5kg of 1000kg water, 8.75kg soybean, pH7.0, through 121 DEG C of real tank sterilizings after 30 minutes, cultivate 48 hours at 28 DEG C～30 DEG C, after mycelium pellet that density is large appears in fermented liquid, be inoculated on fungus solids fermention medium, inoculum size 10%, 121 DEG C, 0.1MPa autoclaving 30min, 25 DEG C of solid fermentations are cultivated 48h.In solid-state fermentation tank, the loading amount of substratum is volume 70%, and sporotrichum thermophile, aspergillus oryzae and viride spore count reach respectively 6 × 10
8individual/g, 5 × 10
8individual/g and 5 × 10
8individual/g.
The subtilis of producing as stated above, described Bacillus licheniformis, described sporotrichum thermophile, described aspergillus oryzae and described viride list bacterial classification weight ratio are to make composite fungus agent after 1～3:0.5～2:1～2:0.5～1:0.5～3 mixing is mixed thoroughly, and composite fungus agent living bacteria count reaches 5 × 10
8individual/g.
embodiment 2:
the production of green bio fertilizer
Under certain site facility condition, there is Multi Role Aircraft material decomposing microbial inoculum by adding, mixing natural organic matter material generation biological degradation, chemical degradation and the physical change of the organic components such as rich cellulose and protein, produce CO
2, H
2o and NH
3deng gas release of heat, generate in a large number and can be transformed and absorb micromolecular inorganic and organic nutrient substance by crop, form stable physical structure and state, obtain green bio organic fertilizer product of the present invention.
1, raw material and formula
Green bio organic fertilizer production raw material comprises brown coal, ground phosphate rock, dregs of beans and four kinds of natural organic materialss of wheat bran.By weight, brown coal account for 40～60%; Ground phosphate rock accounts for 20～40%; Dregs of beans accounts for 5～15%; Wheat bran accounts for 5～15%.
2, material choice
Brown coal organic content >=45%, ground phosphate rock phosphorus pentoxide content >=30%, dregs of beans total nitrogen content >=5%, wheat bran is fresh not snake.
3, proportioning raw materials
Take by weight 40～60% brown coal, 20～40% ground phosphate rock, 5～15% dregs of beans and 5～15% wheat bran.
4, the mixing of raw material
The brown coal that take by weight, ground phosphate rock, dregs of beans and wheat bran are fully mixed with mixer.
5, add water
By water: the weight ratio of mixing raw material 1:1 adds water, control water content in 50～60% left and right.
6, add multi-functional organic material composting microbial inoculum
By multi-functional organic material composting microbial inoculum: the weight ratio of mixing raw material 1:100 adds multi-functional organic material composting microbial inoculum.
7, the specification of banking up
To regulate water and add the organic bank growth of mixing of multi-functional organic material composting microbial inoculum trapezoidal, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, and heap is long to be determined according to place, and every heap volume is no less than 5 sides.
8, the control of turning time
After the fermentation of banking up starts, if fermentation initial stage temperature does not rise, as inadequate in moisture content, adjust moisture to recommendation ratio 50～60%, as excessive in moisture content, must turn over every day and smash once, increase oxygen content in buttress, impel microorganism Fast Growth, until heap temperature rises.In the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once, in order to killing harmful pathogenic micro-organism and the worm's ovums such as excrement enterobacteria and roundworm egg.When heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once.Turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, in the time that temperature exceedes 70 DEG C, need turn over and smash immediately, eliminate the inhibition of high temperature to microorganism growth by cooling, thereby impel the activity of a large amount of mesophilic bacterias, accelerate the process of compost.
9, turning requirement
Turn in buttress process and will accomplish tune, even, broken.Adjusting is exactly original top section in buttress to be transferred to earth's surface become underclad portion, and underclad portion is transferred to top layer and is become top section, to reach the object of abundant fermentation; Even is exactly that buttress Raw is mixed to also inhomogeneous part, again mixes work; Broken is exactly that the massive zymolysis thing of formation is during the fermentation carried out to fragmentation work.
10, fermentation termination control
Heap temperature is identical with external temperature, moisture is while being down to below 30%, outward appearance chocolate, and odorless, has completed the whole fermentation production process of green organic fertilizer, and the whole cycle is generally 15～20 days, and the time in winter is longer.
11, fermented quality control
After having fermented, pulverize, sieve, detect, weigh and pack, green organic manure products production completes.The organic requirement of green organic manure reaches 50%, N, P, K total nutrient 9%, contains abundant amino acid, and aminoacids content reaches 8%, living bacteria count > 2 × 10
8individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
Because described organic material composting microbial inoculum integrates cellulose decomposition, protein decomposition, disease-resistant and bacterial manure several functions, add and mix after natural organic matter material, probiotics on the one hand decomposes humus organic material material and forms the fertilizer of stable high nutrient, utilize organic materials to carry out self expanding propagation on the one hand, in the fertilizer generating, make the fertilizer generating there is equally the multiple performances such as disease-resistant and bacterial manure own disease-resistant and bacterial manure function transplanting.
embodiment 3:
A kind of multi-functional organic material composting composite fungus agent, is formulated as follows:
The composition (by weight) of the multi-functional organic material composting microbial inoculum of table 2
| Title | Subtilis | Bacillus licheniformis | Sporotrichum thermophile | Aspergillus oryzae | Viride |
| （1） | 1.0 | 2.0 | 1.0 | 0.5 | 0.5 |
| （2） | 1.5 | 1.5 | 2.0 | 1.0 | 1.0 |
| （3） | 2.0 | 1.0 | 1.5 | 0.5 | 2.0 |
| （4） | 3.0 | 0.5 | 1.5 | 1.0 | 3.0 |
Wherein living bacteria count is 5 × 10
8individual/g;
The proportioning (percentage ratio by weight) of table 3 natural organic matter material
| Title | Brown coal (%) | Ground phosphate rock (%) | Dregs of beans (%) | Wheat bran (%) |
| （1） | 40 | 40 | 10 | 10 |
| （2） | 45 | 30 | 15 | 10 |
| （3） | 50 | 30 | 5 | 15 |
| （4） | 60 | 20 | 15 | 5 |
embodiment 4:
the using method of green bio fertilizer
Use green bio fertilizer, can increase the supply of plant nutrient, can alleviate again the excessive pollution that chemical fertilizer causes agricultural land soil and water surrounding of using; Both be improved the economic benefit of crops quality and increasing both production and income, had again significant ecological benefits and social benefit.To the protection of agricultural sustainable development and ecotope, and develop pollution-free, pollution-free green food and all have broad prospects.
Be applicable to various vegetables, melon and fruit and food crop, cash crop, and all kinds of crops such as tobacco, tealeaves, herbal medicine, flowers.Administration form is varied, and using method is simple and easy to do.
1, base manure
Use 100～300 kilograms for every mu, before ploughing, spread fertilizer over the fields and earth's surface, then following ploughs is evenly manured into soil and interiorly uses as base manure.
2, seed dressing
After seed is squirted, mix thoroughly with fertilizer, be all stained with fertilizer be advisable with every seed-coat, after seed dressing, dry in the shade (avoiding direct sunlight), sows immediately.3 kilograms of left and right of every mu of fertilizer application amount.Seed dressing is applicable to the crop of direct program request, as cotton, peanut, Chinese cabbage, radish, watermelon, muskmelon etc.
3, seed soaking
Some seed coats are thicker or plant the harder seed of shell, can adopt the method for seed soaking.Specific practice is in the ratio of 1:50, bio-feritlizer to be immersed in water through fully stirring and place 4 hours, gets supernatant liquor and soaks seed 12～24 hours, then through drying in the shade, can sow.3 kilograms of left and right of every mu of fertilizer application amount.
4, be stained with root
In the time that the crops such as vegetables are transplanted seedlings field planting, being stained with root is a kind of important application process.Specific practice be fertilizer and water in the ratio furnishing pasty state of 1:10, and stick in equably on seedling root field planting immediately, mu 5 kilograms of left and right of consumption.
5, fill with root
In the ratio of 1:25, fertilizer is mixed and stirred evenly with water, then pour into crop root, mu 5 kilograms of left and right of consumption.Fill with root and be applicable to fruit tree, vegetables and flowers etc.
6, spread manuer in holes
When dibbling or transplanting, all can take the method for spreading manuer in holes.For ease of fertilising, after fertilizer and fine earth can being mixed, put into every cave, and then transplanting or dibbling, mu 5 kilograms of left and right of consumption.
7, ditch spread
In field of vegetables, trench digging is sprinkled into fertilizer, then moves seedling or dibbling, and its advantage is to spread manuer in holes more simple and easy to doly, is applicable to transplant shallot, garlic etc., mu 8 kilograms of left and right of consumption.
8, spray
Be mainly used in fruit vegetables and fruit tree, tea tree etc.Before bearing fruit He before maturation, spray, can receive positive effect.Concrete grammar is in 1:50 ratio, fertilizer and water fully to be stirred and placed after 1～2 hour, gets supernatant liquor and sprays on crop blade face, and throw out can apply crop root.
9, for seedbed and Nutrition Soil
When raising rice seedlings, be sprinkling upon seedling bed surface by 30 kilograms, every mu of seedbed bio-fertilizer, then broadcast sowing seed rice, then cover fine earth and button mulch film; When grow seedlings of vegetable, with fertilizer preparation Nutrition Soil (1:500), can receive remarkable effect of increasing production.
10, for making compost
After fertilizer and farm manure are mixed in 1:500 ratio, bank up, both can make the farm manure fermentation of fully becoming thoroughly decomposed, can improve again the fertilizer efficiency of farm manure.
11, multiple fertilizing method and repeatedly using
In crop time of infertility, can adopt multiple fertilizing method and repeatedly use, can obtain comparatively ideal effect of increasing production.During as cucumber seedling-raising, the soil of growing seedlings is joined biological organic fertilizer; When survival after transplant, spread manuer in holes or be stained with root biological organic fertilizer; After often plucking one batch of melon, spray the multiple fertilizing methods such as biological organic fertilizer and repeatedly use and combine.
embodiment 5:
the disease-resistant test in field of green bio fertilizer
1, test site
Chrysanthemum shop township, Wuqing District, Tianjin is experimental field meta-alkalescence garden loam.
2, varieties of plant: cucumber
3, field design
(1) spread fertilizer over the fields green bio fertilizer 200Kg/ mu when field planting; (2) spread fertilizer over the fields green bio fertilizer 200Kg/ mu+5kg/ mu green bio fertilizer when field planting and be stained with root; (3) contrast.
4, test-results
Table 4 green bio fertilizer field disease resisting effect
| Process | Sickness rate (%) | Relative control effect % | Per mu yield (Kg) | Stimulation ratio % |
| Process 1 | 17.9 | 51.1 | 4983 | 10.2 |
| Process 2 | 15.1 | 58.7 | 5088 | 12.5 |
| Contrast | 36.6 | - | 4521 | - |
Test-results shows, adds green bio fertilizer and has obvious disease resisting effect, processes 1 preventive effect and reaches 51.1%, processes 2 preventive effects and reaches 58.7%.
embodiment 6:
the field volume increase test of green bio fertilizer
one, green cucumber test
1, test site: Beichen District, Tianjin Xi Ditou group
2, trial crops and kind: yellow No. 2 of Tianjin
3, basic condition experimental field
30 old vegetable garden soils, loam, soil fertility belongs to higher level.0.7 mu of test area, community area 16m
2.
4, test design:
If 2 processing: process 1: conventional fertilizer application { contrast }; Process 2: conventional fertilizer application+green bio fertilizer (200 kgs/acre).
5, test-results
Impact and the Economic and Efficiency Analysis of table 5 green bio fertilizer on cucumber yield
The processing that green bio fertilizer is used in upper table explanation increases output 1014kg than contrast mu, and increasing degree is 26.93%.
two, tomato planting test
1, test site
Test is carried out after Ninghe County, Tianjin reed platform is thin, and test kind is tomato.
2, basic condition experimental field
Middle loamy texture moisture soil, community area 9m
2, the soil organism 2.10%, full nitrogen 0.080%, alkali-hydrolyzable nitrogen 105mgKg
-1, rapid available phosphorus 39mgKg
-1, available potassium 150 mgKg
-1, two ammonium 150 Kg/hm
2, urea 150 Kg/hm
2.
3, test design
If 2 processing: 1. contrast; 2. enrich green bio fertilizer (200 kgs/acre).Processing 3 repetitive sequences for two arranges.
4, test-results
(1) impact of green bio fertilizer on tomato Growth Character
The impact of table 6 green bio fertilizer on tomato Growth Character
Can find out, green bio fertilizer has improved tomato biological proterties, and plant height has increased 0.26m than contrast, and stem slightly increases 0.18cm, and chlorophyll improves 2.99%, and fruit diameter increases 1.59cm.
(2) impact of green bio fertilizer on tomato yield
The impact of table 7 green bio fertilizer on tomato yield
Tomato output per hectare increases 3.108T, and volume increase 7.47%, uses green bio fertilizer, not only volume increase, and have improvement soil, and improve crop quality, crop tamato fruit mouthfeel is good, and t value assay, reaches conspicuous level between processing.
embodiment 7:
impact on the quality test of green bio fertilizer
1, test site
Lan Zhouwan township, Manas County Lanzhou Wan Ercun
2, trial crops: tomato 87-5
3, basic condition experimental field
Loamy texture desert grey soil, soil fertility level is medium on the lower side.Conventional fertilizer application amount two ammonium 15 kg/mus, phosphate fertilizer 30kg/ mu, urea 30kg/ mu.
4, test design
If 3 processing: (1) conventional fertilizer application (contrast); (2) conventional fertilizer application+100kg/ mu green bio fertilizer bottom application; (3) the organic fat bottom application+5kg/ mu green bio fertilizer of conventional fertilizer application+100kg/ mu green bio is stained with root
5, test-results
The impact of table 8 green bio fertilizer on Tomato Quality
| Process | Total reducing sugar (%) | Soluble solid (%) | Haematochrome (mg/100g) | Nitrate (mg/kg) |
| Process 1 | 3.34 | 6.00 | 11.50 | 36.70 |
| Process 2 | 4.08 | 7.08 | 13.20 | 36.30 |
| Process 3 | 3.76 | 6.25 | 13.30 | 31.30 |
Experimental result shows, use total reducing sugar after green bio fertilizer, soluble solid, haematochrome content all high than contrast, and the content of objectionable impurities nitrate declined, and illustrates that using this fertilizer can improve the quality of tomato.
Claims (6)
1. a green bio fertilizer, is characterized in that it is to be made up of multi-functional organic material composting bacteria fermentation natural organic matter material, and wherein the ratio of weight and number of multi-functional organic material composting microbial inoculum and natural organic matter material is 1:100; Green bio fertilizer living bacteria count is greater than 2 × 10
8individual/g; The parts by weight of described organic material composting microbial inoculum are as follows:
Subtilis 1～3 Bacillus licheniformis 0.5～2
Sporotrichum thermophile 1～2 aspergillus oryzae 0.5～1
Viride 0.5～3;
The parts by weight of described natural organic matter material are as follows: 5～15 parts, 40～60 parts, brown coal, 20～40 parts, ground phosphate rock, 5～15 parts of dregs of beans and wheat bran.
2. green bio fertilizer claimed in claim 1, wherein brown coal organic content >=45, ground phosphate rock phosphorus pentoxide content >=30%, dregs of beans total nitrogen content >=5%, wheat bran is fresh not snake.
3. the production method of green green bio fertilizer described in claim 1, is characterized in that being undertaken by following step:
(1) raw material and formula: the brown coal that take by weight, ground phosphate rock, dregs of beans and wheat bran are fully mixed with mixer, and wherein brown coal account for 40～60 parts; Ground phosphate rock accounts for 20～40 parts; Dregs of beans accounts for 5～15 parts; Wheat bran accounts for 5～15 parts; Brown coal organic content >=45%, ground phosphate rock phosphorus pentoxide content >=30%, dregs of beans total nitrogen content >=5%, wheat bran is fresh not snake;
(2) add water: the weight ratio that is 1:1 by water and mixing raw material ratio of weight and number adds water, control water content 50～60%;
(3) add multi-functional organic material composting microbial inoculum: the ratio taking ratio of weight and number as 1:100 adds multi-functional organic material composting microbial inoculum with mixing raw material by organic material composting microbial inoculum;
(4) specification of banking up: will regulate water and add the organic bank of mixing of multi-functional organic material composting microbial inoculum to grow up trapezoidal, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, heap is long to be determined according to place, and every heap volume is no less than 5 sides;
(5) control of turning time: after the fermentation of banking up starts, if fermentation initial stage temperature does not rise, as inadequate in moisture content, adjust moisture to recommendation ratio 50～60%, as excessive in moisture content, must turn over every day and smash once, increase oxygen content in buttress, impel microorganism Fast Growth, until heap temperature rises;
In the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once, in order to killing harmful pathogenic micro-organism and the worm's ovums such as excrement enterobacteria and roundworm egg;
When heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once;
Turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, in the time that temperature exceedes 70 DEG C, need turn over and smash immediately, eliminate the inhibition of high temperature to microorganism growth by cooling, thereby impel the activity of a large amount of mesophilic bacterias, accelerate the process of compost;
(6) turning requires: turn in buttress process and will accomplish tune, even, broken; Adjusting is exactly original top section in buttress to be transferred to earth's surface become underclad portion, and underclad portion is transferred to top layer and is become top section; Even is exactly that buttress Raw is mixed to also inhomogeneous part, again mixes work; Broken is exactly that the massive zymolysis thing of formation is during the fermentation carried out to fragmentation work;
(7) fermentation termination control: heap temperature is identical with external temperature, moisture is while being down to below 30%, outward appearance chocolate, odorless, has completed the whole fermentation production process of green organic fertilizer, and the whole cycle is generally 15～20 days;
(8) fermented quality control: pulverize after having fermented, sieve, detect, weigh and pack, green organic manure products production completes; Wherein organic requirement of green organic manure reaches 50%, N, P, K total nutrient 9%, contains abundant amino acid, and aminoacids content reaches 8%, living bacteria count > 2 × 10
8individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
4. the application of green bio fertilizer in Disease Resistance of Cucumber described in claim 1.
5. the application of green bio fertilizer aspect raising cucumber and tomato yield described in claim 1.
6. the application of green bio fertilizer aspect raising tomato quality described in claim 1.
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