CN102057833A

CN102057833A - Application method of AM (Arhusclar Mycorrhiza) fungi in cultivation of tree peony

Info

Publication number: CN102057833A
Application number: CN2010105417573A
Authority: CN
Inventors: 吴计划
Original assignee: BEIJING TONGRENTANG ANHUI CHINESE MEDICINE RAW MATERIALS Co Ltd
Current assignee: BEIJING TONGRENTANG ANHUI CHINESE MEDICINE RAW MATERIALS Co Ltd
Priority date: 2010-11-12
Filing date: 2010-11-12
Publication date: 2011-05-18

Abstract

The invention belongs to the field of cultivation methods of tree peony and discloses the application method of AM (Arhusclar Mycorrhiza) fungi in the cultivation of tree peony. The application method comprises the following steps of: (1) material preparation; (2) substrate and holed plate sterilization; (3) inoculant preparation; (4) mycorrhiza seedling production; and (5) field transplanting and setting. When used in the cultivation of tree peony, the application method can improve the micro-ecological environment of tree peony rhizosphere, and especially has obvious alleviation effects on the aggravation of plant diseases and pest damages, untimely defoliation and other symptoms, which are caused by many-year continuous cropping, thereby achieving the dual purposes of improving the yield and the quantity of tree peony.

Description

The application method of AM mycorhiza bacterium in the tree peony cultivation

Technical field

The invention belongs to tree peony cultivation method field, be specifically related to the application method of a kind of AM mycorhiza bacterium in the tree peony cultivation.

Background technology

At present, traditional cultivation management method is continued to use in each big tree peony producing region of China basically.Although the part technology improves to some extent and innovates in recent years, totally there is not too big breakthrough.Tree peony main producing region particularly, because continuous cropping for many years, continuous cropping, soil is serious degradation, various damage by disease and insect have become the huge bottleneck that influence tree peony output and quality raising along with increasing of the farming time limit is more and more serious.At above problem, the tree peony expert proposed many schemes, cultivated as no soil for peony.Soilless culture is as the good mode of production of a kind of modernization, though confirm successfully feasiblely technically, distinct issues are that cost height, one-time investment are big; The managerial skills of also having relatively high expectations simultaneously, administrative staff must possess certain scientific knowledge, and this neither can both accomplish Anywhere.

Mycorhiza is the mutualistic symbiosis body that a class fungi and root system of plant form in the soil, extensively exists at natural world, and the AM mycorhiza is one of them main type.Discover that most cereal crops, oil crop, vegetable crop, fruit tree, medicinal plant, pasture crop and some other economic crops can form the AM mycorhiza.The AM mycorhiza can improve the survival rate of transplanted seedling, shortens nursery stage; Improve the absorption of plant, reduce applying quantity of chemical fertilizer phosphorus and other nutritive elements (as N, K, Ca, Mg, Cu, Fe, S, B etc.); Improve the tolerance of plant to adverse circumstance such as hot and cold, salt, alkali, heavy metal pollution etc.; Improve little ecological condition of rhizosphere, improve the resistance of plant, reduce root disease, and then reduce the use of part agricultural chemicals soil-borne disease; Bloom in advance, the result, promote fruit maturation, improve the output of crop, improve product quality etc.The inventor has invented the application method of a kind of AM mycorhiza bacterium in the tree peony cultivation for many years by AM mycorhiza bacterium being applied to the experiment and the research of tree peony cultivation.

Summary of the invention

The objective of the invention is to solve symptoms such as damage by disease and insect aggravation that tree peony causes because of continuous cropping for many years and untimely defoliation, thus output and the quality of raising tree peony.

For solving above technical problem, the application method of a kind of AM mycorhiza of the present invention bacterium in the tree peony cultivation may further comprise the steps:

1, raw material is prepared: clover seed, acaulospora specie and ground sacculus mould species, be that forest humus and the river sand mixture of 6:1 made matrix, coil in the cave with volume ratio;

2, matrix and cave dish sterilization: matrix is evenly admixed peat with 0.1% carbendazim solution, and covered with plastic film 1 day then with repeatedly drip washing of clear water, after drying, was sterilized 20 minutes to matrix for 132 ℃ with the autoclave sterilization pot continuously; The cave dish cleans up, and dries after 30 minutes with the immersion of 0.1% liquor potassic permanganate;

3, Inoculant preparation: with clover is host plant, in cultivation tray, spread matrix, AM bacterium, matrix, clover seed, matrix from the bottom up, water permeable back and guarantee that 25 ℃～30 ℃ of daytimes, night are more than l5 ℃, cultivate and after 3～5 months trefoil acrial part is all cut, the matrix mixing that will contain the clover root system of fungal spore, the outer mycelia of root, infection is mixed thoroughly, as Inoculant;

4, the mycorhiza seedling is produced: earlier matrix is loaded on the cave dish, water permeable then, extrude dark about 1 centimetre cave thereon, the Inoculant that accounts for matrix weight about 5% in the dish of cave is evenly spread thereon, will be planted in through 100 times of solution seed soakings of 40% formalin and with the Paeonia suffruticosa seed that clear water washes down then on the dish of cave, cover matrix on it, afterwards the cave dish is put into seedling cultivation greenhouse, 18-20 ℃ of seedling cultivation greenhouse mean temperature grown seedlings 45-60 days, height of seedling 3-5cm and gives birth to 3 leaflets;

5, field-transplanting and field planting: to field soil with mix bacterium sterilization of plastic mulching, making 20 cm layer of soil temperature reach 40 ℃ kept 7 days, plough then 30-40 centimetre dark, watered in airing 3-5 days permeable, with field planting in the field soil of the AM mycorhiza transplantation of seedlings in the greenhouse to the sterilization.

Above-mentioned a kind of AM mycorhiza bacterium is sterilized with the plastic mulching bacterium of mixing to field soil in described field-transplanting and the field planting process in the application method in the tree peony cultivation, can be to make 20 cm layer of soil temperature reach 37 ℃ to keep 20 days.

In the tree peony cultivation, use preceding method can improve the micro-ecological environment of tree peony rhizosphere, especially the remission effect of symptoms such as damage by disease and insect aggravation that tree peony is caused because of continuous cropping for many years and untimely defoliation is remarkable, thereby can reach the dual purpose of raising tree peony output and quality.

Embodiment

Acaulospora specie of the present invention, the sacculus mould species be to buy from Inst. of Plant Nutrition ﹠. Resource, Beijing City Academy of Agricultural ﹠. Fo, be used to carry out the target bacterial classification and expand numerous and Inoculant production.With volume ratio is that forest humus and the river sand mixture of 6:1 made matrix, with the pouring of Hoagland nutrient solution.

1, matrix and cave dish are carried out disinfection.Matrix is evenly admixed peat with 0.1% carbendazim solution, and covered with plastic film 1 day then with repeatedly drip washing of clear water, after drying, was sterilized 20 minutes venting naturally for 132 ℃ continuously to matrix with the autoclave sterilization pot; The cave dish cleans up the back and dries use with the immersion of 0.1% liquor potassic permanganate after 30 minutes.

2, Inoculant preparation.With clover is host plant, in cultivation tray, spread matrix, AM bacterium, matrix, clover seed, matrix from the bottom up, water permeable back and guarantee that 25 ℃～30 ℃ of daytimes, night are more than l5 ℃, cultivate after 3～5 months, trefoil acrial part is all cut and the clover root system that infects is cut into root segment about 2,3 centimetres with the scissors after the sterilization.This root segment and the matrix mixing that contains fungal spore, the outer mycelia of root are mixed thoroughly, as AM mycorhiza bacterium Inoculant.

3, the mycorhiza seedling is produced.The matrix of will sterilizing earlier is loaded on the cave dish, water permeable then, extrude dark about 1 centimetre cave thereon, the inoculation microbial inoculum that accounts for seedling medium weight about 5% in the dish of cave is evenly spread thereon, to be planted in through 100 times of solution seed soakings of 40% formalin and with the Paeonia suffruticosa seed that clear water washes down then on the dish of cave, cover sterilization matrix on it, the cave dish can be put into seedling cultivation greenhouse afterwards.18-20 ℃ of seedling cultivation greenhouse mean temperature, with about above point disk seedling growing 45-60 days tree peony root system and AM mycorhiza bacterium are formed and well infect symbiotic relation, height of seedling 3-5cm and gives birth to 3 leaflets, enter the seedling phase, obtain the tree peony cave cup fungi offspring of well-grown stalwartness.

4, the field-transplanting of tree peony mycorhiza seedling and field planting.Carry out the assorted bacterium sterilization of high temperature to transplanting soil, form airtight greenhouse, make 20 cm layer of soil temperature reach 40 ℃ and kept 7 days, perhaps make 20 cm layer of soil temperature reach 37 ℃ and kept 20 days, can effectively kill pests such as bacterium in the soil, root-knot nematode with plastic mulching.Sterilization is ploughed after finishing, plough the degree of depth and be advisable with 30-40 centimetre, in case the deep subsoil biology is translated into the face of land, water after airing 3-5 days permeable, with the field planting to the disinfection soil of the mycorhiza transplantation of seedlings in the greenhouse.

The mycorhiza transplantation of seedlings to because of the serious soil of continuous cropping tree peony soil-borne disease insect pest for many years, can be alleviated soil continuous cropping obstacle, make the tree peony growth be better than common seedling, and then improve the plant vigor, the accumulation that increases active ingredients such as photosynthetic yield and Paeonol; In addition, use the mycorhiza seedling to meet the needs of environmental protection agricultural, reduced applying quantity of chemical fertilizer, improved the rich water availability greatly.

Claims

1. the AM mycorhiza bacterium application method in the tree peony cultivation is characterized in that this method may further comprise the steps:

(1) raw material is prepared: clover seed, acaulospora specie, the sacculus mould species, be that forest humus and the river sand mixture of 6:1 made matrix, coil in the cave with volume ratio;

(2) matrix and cave dish sterilization: matrix is evenly admixed peat with 0.1% carbendazim solution, and covered with plastic film 1 day then with repeatedly drip washing of clear water, after drying, was sterilized 20 minutes to matrix for 132 ℃ with the autoclave sterilization pot continuously; The cave dish cleans up, and dries after 30 minutes with the immersion of 0.1% liquor potassic permanganate;

(3) Inoculant preparation: with clover is host plant, in cultivation tray, spread matrix, AM bacterium, matrix, clover seed, matrix from the bottom up, water permeable back and guarantee that 25 ℃～30 ℃ of daytimes, night are more than l5 ℃, cultivate and after 3～5 months trefoil acrial part is all cut, the matrix mixing that will contain the clover root system of fungal spore, the outer mycelia of root, infection is mixed thoroughly, as mycorhiza bacterium Inoculant;

(4) the mycorhiza seedling is produced: earlier matrix is loaded on the cave dish, water permeable then, extrude dark about 1 centimetre cave thereon, the Inoculant that accounts for matrix weight about 5% in the dish of cave is evenly spread thereon, will be planted in through 100 times of solution seed soakings of 40% formalin and with the Paeonia suffruticosa seed that clear water washes down then on the dish of cave, cover matrix on it, afterwards the cave dish is put into seedling cultivation greenhouse, 18-20 ℃ of seedling cultivation greenhouse mean temperature grown seedlings 45-60 days, height of seedling 3-5cm and gives birth to 3 leaflets;

(5) field-transplanting and field planting: to field soil with mix bacterium sterilization of plastic mulching, making 20 cm layer of soil temperature reach 40 ℃ kept 7 days, plough then 30-40 centimetre dark, watered in airing 3-5 days permeable, with field planting in the field soil of the AM mycorhiza transplantation of seedlings in the greenhouse to the sterilization.

2. application method as claimed in claim 1 is characterized in that in described field-transplanting and the field planting process field soil making 20 cm layer of soil temperature reach 37 ℃ and keeping 20 days with the plastic mulching bacterium sterilization of mixing.