CN102715004B - A kind of method that improves ornamental plant resistance - Google Patents
A kind of method that improves ornamental plant resistance Download PDFInfo
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- CN102715004B CN102715004B CN201210220663.5A CN201210220663A CN102715004B CN 102715004 B CN102715004 B CN 102715004B CN 201210220663 A CN201210220663 A CN 201210220663A CN 102715004 B CN102715004 B CN 102715004B
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000002689 soil Substances 0.000 claims abstract description 54
- 241000233866 Fungi Species 0.000 claims abstract description 47
- 239000000725 suspension Substances 0.000 claims abstract description 37
- 238000002347 injection Methods 0.000 claims abstract description 20
- 239000007924 injection Substances 0.000 claims abstract description 20
- 230000035784 germination Effects 0.000 claims abstract description 14
- 235000013311 vegetables Nutrition 0.000 claims abstract description 12
- 235000016709 nutrition Nutrition 0.000 claims abstract description 11
- 230000035764 nutrition Effects 0.000 claims abstract description 11
- 239000003415 peat Substances 0.000 claims abstract description 11
- 239000007921 spray Substances 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000006835 compression Effects 0.000 claims description 8
- 238000007906 compression Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 24
- 230000000694 effects Effects 0.000 description 9
- 238000011160 research Methods 0.000 description 4
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000000950 Hippophae rhamnoides Species 0.000 description 1
- 235000003145 Hippophae rhamnoides Nutrition 0.000 description 1
- 241001233115 Paxillus involutus Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Abstract
Improve a method for ornamental plant resistance, belong to ornamental plant resist technology field. Comprise the following steps: 1) prepare VA Mycorrhizal Fungi spore suspension; 2) vegetable seeds vernalization at 23-28 DEG C, when showing money or valuables one carries unintentionally 1/2 time, seed soaks 5-12min in VA Mycorrhizal Fungi suspension, then sow peat: Nutrition Soil volume ratio is that 3-5:1 cultivates in soil, after germination 60-80 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 2-6ml/ strain from nursery stock root 2-3cm place, then cover and compress injection place with cultivating soil, can transplant after being cultured to the 6-8 month, after transplanting, spray again VA Mycorrhizal Fungi spore suspension. A kind of above-mentioned method that improves ornamental plant resistance, has and promotes the synthetic of seed vitality, raising seedling mycorhiza, promotes seedling growth, and the multiple efficacies such as the resistance of raising plant, can effectively resist the generation of various diseases, have special efficacy to improving sapling resistance.
Description
Technical field
The invention belongs to ornamental plant resist technology field, be specially a kind of method that improves ornamental plant resistance.
Background technology
VA Mycorrhizal Fungi (mycorrhizalfungi) is a parasymbiosis fungi that can form with the tender part of the children of plant rhizosphere homobium in soil, has and improves resistance (comprising drought resistance, salt-resistance and disease resistance etc.), the promotion seedling growth of host plant and strengthen host to Cd2+Deng the contamination resistance of heavy metal. The Song Fu research such as by force thinks, the survival rate of Mycorrhizal afforestation on average, more than 95%, can reach the successful effect of once afforesting, and is an indispensable biotechnology in production of forestry. Tang Ming etc. investigate from mycorrhizal fungi resource the many semiarid zones of NORTHWEST CHINA, further investigate the drought resistance impact on plants such as sea-buckthorns from mycorrhizal fungi, and inquire into the mechanism that improves willow Canker-Resistance from branch VA Mycorrhizal Fungi, promoted to a certain extent the development and utilization of China's mycorhiza resource. As the typical Applying Ectomycorrhizal Fungi of one,Paxillusinvolutus(BatschexFr.) Fr. distribution is on the Northern Hemisphere very general, and inoculation is rear than being easier to form mycorhiza. Due toP.involutusCan effectively improve the anti-adversity of host plant, and promote the growth of host plant, exist both at home and abroadP.involutusDevelopment and utilization aspect carried out more research, and obtained larger progress. Quoreshi and Khasa research discovery, whenP.involutusWith bacteriumBurkholderiacepaciaWhile inoculation together, can improve significantly white poplarP.tremuloidesWith face cream poplarP.angustifoliaTo the absorption of the nutrient such as N and P, thus the growth that improves root system. The researchs such as Garbaye think,P.involutusThe mycorhiza forming can improve significantlyQuercuspetraeaWithQ.roburThe drought-resistant ability of tree. In addition,P.involutusRadioactive element Cs-137 is also had to very strong absorption, and can effectively improve the anti-Cu of host2+、Cd2+And Pb2+Deng the ability of heavy metal pollution. The conversion transport of the stable and energy of mycorhiza to forest ecosystem plays positive facilitation, and it is the special parasitics of one that plant and microorganism reach high unity. A large amount of mycorhiza application study both at home and abroad shows, mycorhiza can obviously promote the growth of host plant, improves seedling quality, promotes the absorption of host to nitrogen, phosphorus, potassium, strengthens stress resistance of plant, improves host plant rhizospheric environment etc. Whether this beneficial effect of mycorhiza can be given full play to, and is decided by that can correctness and the mycorrhizal fungi of mycorrhizal fungi selection effectively infect host plant. For different seeds, Bole capacitance, Relative turgidity of bark, peroxidase activity, polyphenol oxidase activity and PAL activity index and trees disease resistance or resistance have close relationship.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of technical scheme of the method that improves ornamental plant resistance, has cost low, and effect is remarkable, natural environmental-protective, the advantage such as without any side effects.
A kind of described method that improves ornamental plant resistance, is characterized in that comprising the following steps:
1) bacterium solution preparation
Take VA Mycorrhizal Fungi Bao ︰ distilled water=1-3:4-6 according to weight ratio, mix and make VA Mycorrhizal Fungi spore suspension;
2) Mycorrhizal processing
Vegetable seeds vernalization at 23-28 DEG C, when showing money or valuables one carries unintentionally 1/2 time, seed soaks 5-12min in VA Mycorrhizal Fungi spore suspension, then sow peat: Nutrition Soil volume ratio is that 3-5:1 cultivates in soil, after germination 60-80 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 2-6ml/ strain from nursery stock root 2-3cm place, then cover compression injection place with cultivating soil, be cultured to after the 6-8 month and can transplant to any soil and cultivate, after transplanting, in Yong Mei ㎡ soil, evenly spray 2-4L VA Mycorrhizal Fungi spore suspension again, after this normal cultivation.
A kind of described method that improves ornamental plant resistance, is characterized in that vegetable seeds germination temperature is 24-27 DEG C, preferably 25-26 DEG C; Soak time is 6-10min, preferably 7-9min.
A kind of described method that improves ornamental plant resistance, is characterized in that cultivating peat in soil: Nutrition Soil volume ratio is 4:1.
A kind of described method that improves ornamental plant resistance, it is characterized in that injecting the bacterium liquid time is 65-75 days after germination, injection bacterium liquid measure is 3-5ml/ strain.
A kind of described method that improves ornamental plant resistance, is characterized in that transplanting time is to cultivate after the 6-8 month, and bacterium liquid sprinkling amount is 2.5-3L/ ㎡.
Above-mentioned VA Mycorrhizal Fungi spore can directly be buied from the market.
A kind of above-mentioned method that improves ornamental plant resistance, taking distilled water as medium, be prepared into suspension bacteria liquid with VA Mycorrhizal Fungi spore, by the bacterium liquid processing to ornamental plant seed and seedling, have and promote the synthetic of seed vitality, raising seedling mycorhiza, promote seedling growth, the multiple efficacies such as the resistance of raising plant, can effectively resist the generation of various diseases, have special efficacy to improving sapling resistance. Its natural environmental-protective, without any pollution, and uses safety to environment, and effect is remarkable, with low cost, is adapted at ornamental plant plantation, transplants and cultivates upper application.
The percentage composition relating in present specification unless otherwise indicated, other the weight percentage that is pure material.
Detailed description of the invention
Now, in conjunction with embodiments of the invention, further illustrate beneficial effect of the present invention.
Embodiment 1
Mix and make VA Mycorrhizal Fungi spore suspension according to VA Mycorrhizal Fungi Bao ︰ distilled water=2:5 ratio, peat: Nutrition Soil=4:1 makes cultivation soil by volume again, then by vegetable seeds vernalization at 23 DEG C, put into VA Mycorrhizal Fungi spore suspension 1/2 time and soak 5min when seed shows money or valuables one carries unintentionally, then sow in cultivation soil, Deng germination after 60 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 6ml/ strain from nursery stock root 3cm place, and then cover compression injection place with cultivating soil, after being cultured to June, transplant to normal soil, after transplanting, in Yong Mei ㎡ soil, evenly spray 2L VA Mycorrhizal Fungi spore suspension again, after this normally cultivation.
Embodiment 2
Mix and make VA Mycorrhizal Fungi spore suspension according to VA Mycorrhizal Fungi Bao ︰ distilled water=3:5 ratio, peat: Nutrition Soil=3:1 makes cultivation soil by volume again, then by vegetable seeds vernalization at 28 DEG C, put into VA Mycorrhizal Fungi suspension 1/2 time and soak 12min when seed shows money or valuables one carries unintentionally, then sow in cultivation soil, Deng germination after 80 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 2ml/ strain from nursery stock root 3cm place, and then cover compression injection place with cultivating soil, after being cultured to August, transplant to normal soil, after transplanting, in Yong Mei ㎡ soil, evenly spray 4L VA Mycorrhizal Fungi spore suspension again, after this normally cultivation.
Embodiment 3
Mix and make VA Mycorrhizal Fungi spore suspension according to VA Mycorrhizal Fungi Bao ︰ distilled water=4:6 ratio, peat: Nutrition Soil=4:1 makes cultivation soil by volume again, then by vegetable seeds vernalization at 24 DEG C, put into VA Mycorrhizal Fungi suspension 1/2 time and soak 6min when seed shows money or valuables one carries unintentionally, then sow in cultivation soil, Deng germination after 65 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 3ml/ strain from nursery stock root 3cm place, and then cover compression injection place with cultivating soil, after being cultured to July, transplant to normal soil, after transplanting, in Yong Mei ㎡ soil, evenly spray 3L VA Mycorrhizal Fungi spore suspension again, after this normally cultivation.
Embodiment 4
Mix and make VA Mycorrhizal Fungi spore suspension according to VA Mycorrhizal Fungi Bao ︰ distilled water=2:5 ratio, peat: Nutrition Soil=5:1 makes cultivation soil by volume again, then by vegetable seeds vernalization at 25 DEG C, put into VA Mycorrhizal Fungi suspension 1/2 time and soak 7min when seed shows money or valuables one carries unintentionally, then sow in cultivation soil, Deng germination after 70 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 4ml/ strain from nursery stock root 3cm place, and then cover compression injection place with cultivating soil, after being cultured to 6.5 months, transplant to normal soil, after transplanting, in Yong Mei ㎡ soil, evenly spray 2.5L VA Mycorrhizal Fungi spore suspension again, after this normally cultivation.
Embodiment 5
Mix and make VA Mycorrhizal Fungi spore suspension according to VA Mycorrhizal Fungi Bao ︰ distilled water=2:5 ratio, peat: Nutrition Soil=4:1 makes cultivation soil by volume again, then by vegetable seeds vernalization at 26 DEG C, put into VA Mycorrhizal Fungi suspension 1/2 time and soak 8min when seed shows money or valuables one carries unintentionally, then sow in cultivation soil, Deng germination after 75 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 5ml/ strain from nursery stock root 3cm place, and then cover compression injection place with cultivating soil, after being cultured to 7.5 months, transplant to normal soil, after transplanting, in Yong Mei ㎡ soil, evenly spray 3.5L VA Mycorrhizal Fungi spore suspension again, after this normally cultivation.
Embodiment 6
Mix and make VA Mycorrhizal Fungi spore suspension according to VA Mycorrhizal Fungi Bao ︰ distilled water=3:4 ratio, peat: Nutrition Soil=4:1 makes cultivation soil by volume again, then by vegetable seeds vernalization at 27 DEG C, put into VA Mycorrhizal Fungi suspension 1/2 time and soak 10min when seed shows money or valuables one carries unintentionally, then sow in cultivation soil, Deng germination after 72 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 3.5ml/ strain from nursery stock root 3cm place, and then cover compression injection place with cultivating soil, after being cultured to June, transplant to normal soil, after transplanting, in Yong Mei ㎡ soil, evenly spray 2.8L VA Mycorrhizal Fungi spore suspension again, after this normally cultivation.
Further illustrate beneficial effect of the present invention by test below.
Table 1 shows: sapling mycorhiza is synthesized embodiment 1-6 and seedling growth all has good facilitation effect, show and use this Mycorrhizal technology can improve the resistance of plant, strengthen its comprehensive Physiology and biochemistry ability, wherein, embodiment 1 is better than embodiment 2, embodiment 3 is better than embodiment 4, and embodiment 5 is better than embodiment 6.
Table 2 shows: embodiment 1-6 all has good facilitation effect to nursery stock Disease Resistance-Related Enzymes, and wherein, embodiment 1 is better than embodiment 2, and embodiment 3 is better than embodiment 4, and embodiment 5 is better than embodiment 6, is all significantly higher than control group.
Claims (6)
1. improve a method for ornamental plant resistance, it is characterized in that comprising the following steps:
1) bacterium solution preparation
Take VA Mycorrhizal Fungi spore: distilled water=1-3:4-6 according to weight ratio, mix and make VA Mycorrhizal Fungi spore suspension;
2) Mycorrhizal processing
Vegetable seeds vernalization at 23-28 DEG C, when showing money or valuables one carries unintentionally 1/2 time, seed soaks 5-12min in VA Mycorrhizal Fungi spore suspension, then sow peat: Nutrition Soil volume ratio is that 3-5:1 cultivates in soil, after germination 60-80 days, again at the VA Mycorrhizal Fungi spore suspension of using needle cylinder injection 2-6ml/ strain from nursery stock root 2-3cm place, then cover compression injection place with cultivating soil, be cultured to after the 6-8 month and can transplant to any soil and cultivate, after transplanting, in Zai Mei ㎡ soil, evenly spray 2-4L VA Mycorrhizal Fungi spore suspension, after this normal cultivation.
2. a kind of method that improves ornamental plant resistance as claimed in claim 1, is characterized in that vegetable seeds germination temperature is 24-27 DEG C, and soak time is 6-10min.
3. a kind of method that improves ornamental plant resistance as claimed in claim 1, is characterized in that vegetable seeds germination temperature is 25-26 DEG C, and soak time is 7-9min.
4. a kind of method that improves ornamental plant resistance as claimed in claim 1, is characterized in that cultivating peat in soil: Nutrition Soil volume ratio is 4:1.
5. a kind of method that improves ornamental plant resistance as claimed in claim 1, it is characterized in that injecting the bacterium liquid time is 65-75 days after germination, injection bacterium liquid measure is 3-5ml/ strain.
6. a kind of method that improves ornamental plant resistance as claimed in claim 1, is characterized in that transplanting time is to cultivate after the 6-8 month, and bacterium liquid sprinkling amount is 2.5-3L/ ㎡.
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| CN103828618B (en) * | 2014-03-26 | 2016-05-11 | 兰州大学 | A kind of method of utilizing AMF to improve wheat growth and drought-resistant ability |
| CN108668794A (en) * | 2018-05-25 | 2018-10-19 | 浙江吉瑞建设有限公司 | A kind of cultural method of Multifunctional garden landscape plant |
| CN110731217A (en) * | 2018-07-18 | 2020-01-31 | 东北林业大学 | method for improving tree transplanting survival rate by inoculating mycorrhizal fungi |
| CN112740949A (en) * | 2020-12-22 | 2021-05-04 | 西施生态科技股份有限公司 | Cultivation method capable of effectively improving growth performance of stress-resistant arbor seedling plants |
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