CN101239847B - Liquid composite microbial fertilizer and its preparation method - Google Patents
Liquid composite microbial fertilizer and its preparation method Download PDFInfo
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Abstract
A liquid composite microorganism fertilizer and the preparation method thereof belong to the field of the microorganism fertilizer technique. The invention uses four microorganisms of bacillus subtilis, bacillus magaterium, silicate bacterium and Streptomyces Jingyingensis for mixed-zymolysis and is combined with soluble nitrogen, phosphorus and potassium to prepare the liquid composite microorganism fertilizer. The fertilizer can be used for all type of soil and crops, the application effect on vegetable, fruit is optimum, and the fertilizer has the effects of improving the soil structure, increasing the water-keeping and fertilizer-keeping capability of the soil, and better increasing the production and improving the crop quality.
Description
Technical field
The present invention relates to a kind of liquid composite microbial fertilizer and preparation method thereof, belong to the microbial fertilizer technical field.
Background technology
Bei Jielinke in 1891 have finished the pure culture of root nodule bacterium and vinelandii first, have begun the production and the application of microbial fertilizer, commercial rhizobium inoculant in 1895 appear on the market (commodity are called Nitrogen).30～forties of this century, all there have been Inoculant manufacturing enterprise in the U.S., Australia and Britain etc., nineteen thirty the sales volume of U.S.'s rhizobium inoculant reach 1,000,000 dollars.After the fifties in the world more than 70 country comprise China, begin one's study, produce and use rhizobium inoculant, and area enlarges constantly.Begin in the world the seventies beneficial microorganisms such as vinelandii, phosphate-solubilizing bacteria and potassium bacterium have been carried out a series of research work, a large amount of field tests show, vinelandii, phosphate-solubilizing bacteria and potassium bacterium can increase production and culture fertility, and the application of certain area has been arranged.China is in the world in agricultural application microorganism country the earliest, and ancient times, people just had when newly warding off field plantation leguminous plants, used the way of old field (leguminous plants field) soil moved in to improve the original.The existing nearly 50 years history of modern China's research using microbe fertilizer, what begin to use is the rhizobium leguminosarum Inoculant, in the period of the 1949-1955, the area that peanut rhizobium is used in the whole nation reaches 1,000 ten thousand mu according to statistics, 3,425 ten thousand mu of 1956-1985 accumulative totals.The fifties, introduce azotobacter, phosphorus bacteria and silicate bacteria from the Soviet Union, technical measures in " agricultural development programme " were once classified in the application of bacterial fertilizer in 1958 as.The sixties, China carried out the broad research and the application of " 5406 ", had obtained certain effect.Begin the research that some R﹠D institutions have carried out VA mycorhiza, vinelandii, phosphorus bacteria and silicate bacteria the seventies.Enter the nineties, the research of microbial fertilizer and application have started another climax.
Microbial fertilizer is defined as: the particular formulations that contains live microorganism, existing product is the microbial fertilizer of simple function mostly, such as: vinelandii, phosphate solubilizing bacteria or potassium solubilizing bacteria, its function be respectively nitrogen in the fixed air, decompose the indissoluble phosphorus in the soil or decompose potassium-containing compound in the soil.The independent use of these microorganisms all can be brought into play each self-applying, and part satisfies the demand of crop to nutritive elements such as nitrogen, phosphorus, potassium, uses mentioned microorganism fertilizer also can produce green food, and the protection environment is a kind of environmental friendly fertilizer.But its deficiency is: use above-mentioned three class fertilizer can only solve an aspect of plant desired nutritional separately.The researchist has also noticed this problem, has also studied, has used different technology and produce composite microbiological fertilizer in microbial fertilizer production, just a plurality of microbial strainss is combined with each other.Existing Chinese patent file CN1134408A (95104927.5), CN1191854A (98100645.0), CN1587218A (200410052666.8), CN1600751A (200410050683.8), CN1654435A (200410065948.1), CN1911870A (200610068499.5) adopts different microbial strainss, by cultivating respectively, after the fermentation, product is combined composite microbiological fertilizer in proportion, the subject matter that adopts existing processes to produce the microbial fertilizer existence is that the facility investment of the independent fermentation of each bacterial classification is big, production cycle is long, thereby production cost height, production technique are complicated.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of liquid composite microbial fertilizer and preparation method thereof is provided.
The present invention is directed to the problem that present composite microbiological fertilizer exists in producing, adopt the mixed fermentation technology, and compound chemical fertilizer, on composite microbiological fertilizer fermentation raw material, microbial strains and integral production technology, improve.
Summary of the invention
The present invention is with subtilis (Bacillus Subtilis), bacillus megaterium (Bacillus Megaterium), 4 kinds of microorganism mixed fermentations of silicate bacteria (BacillusMucilaginosus) and Jingyang streptomycete (Streptomyces jingyangensis) and compound solubility azophoska make liquid composite microorganism fertilizer, this fertilizer can be used for all soil types and farm crop, vegetables, effect the best on the fruit, it can improve Soil structure, improve the soil water-reataining fertilizer-keeping performance, have better volume increase and improve the effect of crop quality.
Detailed Description Of The Invention
A kind of liquid composite microorganism fertilizer is characterized in that, is mixed and is got by following raw material, is weight part:
The mixed fermentation liquid of 10～20 parts of four kinds of microorganisms, the mixed fermentation liquid of described four kinds of microorganisms is by subtilis, bacillus megaterium, silicate bacteria, Jingyang streptomycete mixed fermentation form;
40～90 parts of water;
10～20 parts of solubility azophoska.
Subtilis (Bacillus Subtilis) wherein, the culture presevation of Chinese agriculture microbial strains preservation administrative center number is withered-3; Bacillus megaterium (Bacillus Megaterium), the culture presevation of Chinese agriculture microbial strains preservation administrative center number is ACCC10011; Silicate bacteria (Bacillus Mucilaginosus), it is ACCC10013 that Chinese agriculture microbial strains preservation administrative center bacterial classification is protected minus sign; Jingyang streptomycete (Streptomyces jingyangensis), the culture presevation of Chinese agriculture microbial strains preservation administrative center number is ACCC40021; Above-mentioned bacterial classification all can be available from Chinese agriculture microbial strains preservation administrative center.
Preferably, viable bacteria amount is respectively in the mixed fermentation liquid of described four kinds of microorganisms: subtilis 50～7,000,000,000 milliliter
-1, bacillus megaterium 50～8,000,000,000 milliliter
-1, silicate bacteria 30～5,000,000,000 milliliter
-1, Jingyang streptomycete 10～2,000,000,000 milliliter
-1, four kinds of total viable bacteria amounts of bacterial classification are not less than 15,000,000,000 milliliters in the mixed fermentation liquid
-1
Described water purification meets drinking water standard.
The weight ratio of the nitrogenous fertilizer in the described solubility azophoska, phosphate fertilizer, potash fertilizer is (1～2): (0.5～1): (1～2);
In the described solubility azophoska, nitrogenous fertilizer is selected from one of urea, ammonium sulfate or combination, and phosphate fertilizer is selected from monoammonium phosphate, diammonium phosphate or combination, and potash fertilizer is selected from one of Repone K, vitriolate of tartar or combination.
A kind of preparation method of liquid composite microorganism fertilizer, step is as follows:
1. the preparation of mixed fermentation liquid
(1) subtilis, bacillus megaterium, silicate bacteria and 4 kinds of microbial strainss of Jingyang streptomycete are carried out shake-flask culture respectively, get subtilis bacterium liquid, bacillus megaterium bacterium liquid, silicate bacteria bacterium liquid and four kinds of bacterium liquid of Jingyang streptomycete bacterium liquid
(2) the subtilis bacterium liquid of step (1), bacillus megaterium bacterium liquid are respectively entered the seeding tank mixing fermentation culture by 3% inoculum size, zymocyte liquid I;
(3) the silicate bacteria bacterium liquid of step (1), Jingyang streptomycete bacterium liquid are respectively entered mixing fermentation culture behind the seeding tank by 3% inoculum size, zymocyte liquid II;
(4) with the zymocyte liquid II of the zymocyte liquid I of step (2) and step (3) respectively by 10%～, 20% inoculum size enters fermentor tank, the while mixed culture, 30 ℃ of culture temperature, cultivate 180～200 rev/mins of rotating speeds, cultivate ventilate 1: 1 (v: v/min), incubation time 48 hours, the inspection bacterium goes out jar and obtains mixed fermentation liquid;
2. water is joined in the above-mentioned mixed fermentation liquid by proportioning, mix evenly, get the aqueous solution of mixed fermentation liquid;
With the nitrogenous fertilizer of solubility, phosphate fertilizer, potash fertilizer by weight being (1～2): (0.5～1): (1～2) mix the solubility azophoska, pulverize, cross 80 mesh sieves;
4. with the aqueous solution of the mixed fermentation liquid of the solubility azophoska of step 3 and step 2 uniform mixing in proportion, all promptly get liquid composite microorganism fertilizer after the dissolving.This product can be packed to detect and dispatch from the factory.
The processing condition of the shake-flask culture of (1) and (2) seeding tank fermentation culture can be by state of the art in the above-mentioned steps 1.The present invention is preferably as follows condition:
(1) shake-flask culture: inject the 150ml substratum to the 500ml triangular flask; With the 5ml sterilized water bacterial classification on the inclined-plane is changed over to and to shake accordingly in the bottle, under 28～30 ℃, 200 rev/mins conditions, cultivate 24h.
(2) seeding tank fermentation culture: 30 ℃ of temperature, 180～200 rev/mins of rotating speeds of cultivation, 1: 1 (v: v/min) of cultivation ventilation; Incubation time 36 hours, 35 hours inspection bacterium.
Preferred all kinds of substratum is as follows, and per-cent wherein all is weight percentage: subtilis, bacillus megaterium seeding tank fermentation culture
Substratum: ammonium sulfate 0.2%
Glucose 0.286%
Potato starch 0.714%
Dipotassium hydrogen phosphate 0.030%
Sodium-chlor 0.020%
Repone K 0.020%
Sal epsom 0.080%
Manganous sulfate 0.003%
Ferrous sulfate 0.003%
Yeast powder 0.300%
Distilled water 98.00%
Silicate bacteria, Jingyang streptomycete seed jar fermentation culture
Substratum: soybean cake powder 0.5%
Sucrose 0.5%
Yeast powder 0.5%
Ammonium sulfate 0.01%
Sal epsom 0.08%
Dipotassium hydrogen phosphate 0.1%
Sodium-chlor 0.01%
Lime carbonate 0.1%
Ferrous sulfate 0.001%
Distilled water 98.2%
Four kinds of bacterium (mixing) produce a jar fermentation culture
Substratum: starch 3%
Soybean cake powder 1%
Dipotassium hydrogen phosphate 0.03%
Ammonium sulfate 0.1%
Sal epsom 0.05%
Iron(ic) chloride 0.001%
Lime carbonate 0.01%
Yeast powder 0.02%
Distilled water 95.8%
In the fermented liquid of the inventive method preparation, the viable bacteria amount reaches 15,000,000,000 milliliters
-1More than, bacillus megaterium 50～7,000,000,000 milliliter wherein
-1, subtilis 50～8,000,000,000 milliliter
-1, silicate bacteria 30～5,000,000,000 milliliter
-1, Jingyang streptomycete 10～2,000,000,000 milliliter
-1
Liquid composite microorganism fertilizer of the present invention, can be used for all soil types and farm crop, effect the best on vegetables, fruit, it can improve Soil structure, improve the soil water-reataining fertilizer-keeping performance, has better volume increase and improves the effect of crop quality.
Excellent results of the present invention is as follows:
1, invention liquid composite microorganism fertilizer bacterial classification mixes together with fermentation, and production unit is few, and cost is low, is easy to realize;
2, the present invention has strengthened the effect of microbial fertilizer with the combining of multiple microorganism, and significantly improves fertilizer utilization ratio, effectively prevents and treats environmental pollution;
3. fertilizer of the present invention can significantly improve crop yield, improves quality of agricultural product;
4. fertilizer of the present invention has the effect of significantly improving the soil, and can improve soil sustainable use ability.
Embodiment
Below in conjunction with embodiment the present invention is further described, but be not limited thereto.If no special instructions, the per-cent in the raw material all is weight percentage.Described raw material is the commercially available prod, and soybean cake powder is crossed 80 mesh sieves, and the nitrogenous fertilizer of solubility, phosphate fertilizer, potash fertilizer are crossed 80 mesh sieves.
The preparation of the mixed fermentation liquid of 1: four kind of microorganism of embodiment
(1) cultivation of subtilis liquid
Substratum: potato (remove the peel, boil) 200 grams
Sucrose (white sugar or glucose) 20 grams
1000 milliliters of distilled water
pH 6.0～6.5
Agar 15～20 grams
Inject the 150ml substratum to the 500ml triangular flask; With the 5ml sterilized water bacterial classification on the inclined-plane is changed over to and to shake accordingly in the bottle, under 28～30 ℃, 200 rev/mins conditions, cultivate 24h.
(2) cultivation of bacillus megaterium liquid
Substratum: extractum carnis 5.0 grams
Peptone 5.0 grams
Sodium-chlor 5.0 grams
pH 7.2～7.4
1000 milliliters of distilled water
Agar 15～18 grams
Inject the 150ml substratum to the 500ml triangular flask; With the 5ml sterilized water bacterial classification on the inclined-plane is changed over to and to shake accordingly in the bottle, under 28～30 ℃, 200 rev/mins conditions, cultivate 24h.
(3) subtilis, bacillus megaterium seeding tank fermentation culture
Substratum: ammonium sulfate 0.2%
Glucose 0.286%
Potato starch 0.714%
Dipotassium hydrogen phosphate 0.030%
Sodium-chlor 0.020%
Repone K 0.020%
Sal epsom 0.080%
Manganous sulfate 0.003%
Ferrous sulfate 0.003%
Yeast powder 0.300%
Distilled water 98.00%
By subtilis liquid, bacillus megaterium liquid respectively is that 3% inoculum size enters mixed fermentation behind the seeding tank; : 30 ℃ of temperature, 180～200 rev/mins of rotating speeds of cultivation, 1: 1 (v: v/min) of cultivation ventilation; Incubation time 36 hours, 35 hours inspection bacterium.
(4) cultivation of silicate bacteria liquid
The culture presevation substratum
Sucrose 10 grams
K
2HPO
40.5 gram
MgSO
47H
2O 0.2 gram
CaCO
31 gram
FeCl
30.005 gram
Yeast powder 0.4 gram
1000 milliliters of distilled water
pH 7.0～7.2
Agar 15～18 grams
Shake-flask culture base (28～30 ℃, 36h, rotating speed are 200 rev/mins)
Sucrose 5 grams
Yeast extract paste 0.8 gram
Ammonium sulfate 0.1 gram
Sal epsom 0.8 gram
K
2HPO
41 gram
Sodium-chlor 0.1 gram
Lime carbonate 1 gram
pH 7.0～7.2
Inject the 150ml substratum to the 500ml triangular flask; With the 5ml sterilized water bacterial classification on the inclined-plane is changed over to and to shake accordingly in the bottle, under 28～30 ℃, 200 rev/mins conditions, cultivate 36h.
(5) substratum of Jingyang streptomycete
Substratum: Zulkovsky starch 20 grams
KNO
3 1.O
K
2HPO
40.5 gram
MgSO
47H
2O 0.5 gram
NaCl 0.5 gram
FeSO
47H
2O 0.01 gram
1000 milliliters of distilled water
pH 7.2～7.4
Agar 15～18 grams
Inject the 150ml substratum to the 500ml triangular flask; With the 5ml sterilized water bacterial classification on the inclined-plane is changed over to and to shake accordingly in the bottle, under 25～28 ℃, 200 rev/mins conditions, cultivate 48h.
(6) silicate bacteria, Jingyang streptomycete seed jar fermentation culture
Substratum: soybean cake powder 0.5%
Sucrose 0.5%
Yeast powder 0.5%
Ammonium sulfate 0.01%
Sal epsom 0.08%
Dipotassium hydrogen phosphate 0.1%
Sodium-chlor 0.01%
Lime carbonate 0.1%
Ferrous sulfate 0.001%
Distilled water 98.2%
By silicate bacteria liquid, Jingyang streptomycete liquid respectively is that 3% inoculum size enters mixed fermentation behind the seeding tank; 30 ℃ of temperature, 190 rev/mins of rotating speeds of cultivation, 1: 1 (v: v/min) of cultivation ventilation; Incubation time 36 hours, 35 hours inspection bacterium.
(7) four kinds of bacterium (mixing) produce a jar fermentation culture
Substratum: starch 3%
Soybean cake powder 1%
Dipotassium hydrogen phosphate 0.03%
Ammonium sulfate 0.1%
Sal epsom 0.05%
Iron(ic) chloride 0.001%
Lime carbonate 0.01%
Yeast powder 0.02%
Distilled water 95.8%
Making 4 class bacterium enter fermentor tank by the inoculum size that respectively is 10%～20% the bacterium liquid of step (3) and (6) cultivates simultaneously, 30 ℃ of culture temperature, 190 rev/mins of rotating speeds of cultivation, cultivation are ventilated: 1: 1 (v: v/min), cultivated 48 hours, the inspection bacterium goes out the fermented liquid that jar obtains four kinds of microorganisms.
The viable bacteria amount reaches 15,000,000,000 milliliters in the fermented liquid of these four kinds of microorganisms
-1More than, wherein bacillus megaterium is 7,000,000,000 milliliters
-1, 4,000,000,000 milliliters of subtilises
-1, 3,000,000,000 milliliters of silicate bacterias
-1, 1,000,000,000 milliliters of Jingyang streptomycetes
-1);
Embodiment 2: liquid composite microorganism fertilizer
10 kilograms of the fermented liquids of four kinds of microorganisms of embodiment 1 preparation, 90 kilograms of water purification are pulverized 12.28 kilograms of the solubility azophoska (wherein, 6.76 kilograms in ammonium sulfate, 1.32 kilograms in urea, 2.2 kilograms of diammonium phosphate, 2.0 kilograms of vitriolate of tartar) that 80 mesh sieves are crossed in the back.
Preparation method: water purification is joined in the fermented liquids of four kinds of microorganisms and mix, in the aqueous solution of the fermented liquid that the solubility azophoska is dissolved in four kinds of microorganisms, mix, can pack to detect and dispatch from the factory.
This fertilizer is suitable for field crop, facilities vegetable and outdoor vegetable.
Embodiment 3:
With embodiment 2, different is 10.84 kilograms of solubility azophoska, the consisting of of solubility azophoska: 7.64 kilograms in ammonium sulfate, 2.2 kilograms of diammonium phosphate, 2.0 kilograms of vitriolate of tartar.
Embodiment 4:
With embodiment 2, different is 7.7 kilograms of solubility azophoska, the consisting of of solubility azophoska: 3.5 kilograms in urea, 2.2 kilograms of diammonium phosphate, 2.0 kilograms of vitriolate of tartar.
Claims (5)
1. liquid composite microorganism fertilizer, it is characterized in that, mix and get by following raw material, be weight part: the mixed fermentation liquid of 10～20 parts of four kinds of microorganisms, the mixed fermentation liquid of described four kinds of microorganisms is by subtilis (Bacillus Subtilis), bacillus megaterium (Bacillus Megaterium), silicate bacteria (BacillusMucilaginosus), Jingyang streptomycete (Streptomyces jingyangensis) mixed fermentation form;
40～90 parts of water;
10～20 parts of solubility azophoska;
The mixed fermentation liquid of described four kinds of microorganisms prepares according to the following steps:
(1) subtilis, bacillus megaterium, silicate bacteria and 4 kinds of microbial strainss of Jingyang streptomycete are carried out shake-flask culture respectively, get subtilis bacterium liquid, bacillus megaterium bacterium liquid, silicate bacteria bacterium liquid and four kinds of bacterium liquid of Jingyang streptomycete bacterium liquid
(2) the subtilis bacterium liquid of step (1), bacillus megaterium bacterium liquid are respectively entered the seeding tank mixing fermentation culture by 3% inoculum size, zymocyte liquid I;
(3) the silicate bacteria bacterium liquid of step (1), Jingyang streptomycete bacterium liquid are respectively entered mixing fermentation culture behind the seeding tank by 3% inoculum size, zymocyte liquid II;
(4) the zymocyte liquid II of the zymocyte liquid I of step (2) and step (3) is respectively entered fermentor tank by 10%～20% inoculum size, the while mixed culture, 30 ℃ of culture temperature, cultivate 180～200 rev/mins of rotating speeds, cultivate and ventilate 1: 1v: v/min, incubation time 48 hours, the inspection bacterium goes out jar and obtains mixed fermentation liquid.
2. a kind of liquid composite microorganism fertilizer as claimed in claim 1 is characterized in that, viable bacteria amount is respectively in the mixed fermentation liquid of described four kinds of microorganisms: subtilis 50～7,000,000,000 milliliter
-1, bacillus megaterium 50～8,000,000,000 milliliter
-1, silicate bacteria 30～5,000,000,000 milliliter
-1, Jingyang streptomycete 10～2,000,000,000 milliliter
-1, four kinds of total viable bacteria amounts of bacterium are not less than 15,000,000,000 milliliters in the mixed fermentation liquid
-1
3. a kind of liquid composite microorganism fertilizer as claimed in claim 1 is characterized in that, the weight ratio of the nitrogenous fertilizer in the described solubility azophoska, phosphate fertilizer, potash fertilizer is 1～2: 0.5～1: 1～2.
4. a kind of liquid composite microorganism fertilizer as claimed in claim 1, it is characterized in that in the described solubility azophoska, nitrogenous fertilizer is selected from one of urea, ammonium sulfate or combination, phosphate fertilizer is selected from monoammonium phosphate, diammonium phosphate or combination, and potash fertilizer is selected from one of Repone K, vitriolate of tartar or combination.
5. the preparation method of the liquid composite microorganism fertilizer of a claim 1, step is as follows:
(1) preparation of mixed fermentation liquid
(1) with subtilis, bacillus megaterium,, silicate bacteria and 4 kinds of microbial strainss of Jingyang streptomycete carry out shake-flask culture respectively, subtilis bacterium liquid, bacillus megaterium bacterium liquid, silicate bacteria bacterium liquid and four kinds of bacterium liquid of Jingyang streptomycete bacterium liquid; Above-mentioned shake-flask culture adopts following condition: inject the 150ml substratum to the 500ml triangular flask; With the 5ml sterilized water bacterial classification on the inclined-plane is changed over to and to shake accordingly in the bottle, under 28～30 ℃, 200 rev/mins conditions, cultivate 24h;
(2) the subtilis bacterium liquid of step (1), bacillus megaterium bacterium liquid are respectively entered the seeding tank mixing fermentation culture by 3% inoculum size, zymocyte liquid I; Above-mentioned seeding tank fermentation culture adopts following condition: 30 ℃ of temperature, 180～200 rev/mins of rotating speeds of cultivation, cultivation ventilate 1: 1v: v/min; Incubation time 36 hours, 35 hours inspection bacterium; Above-mentioned seeding tank fermention medium is as follows:
Ammonium sulfate 0.2%
Glucose 0.286%
Potato starch 0.714%
Dipotassium hydrogen phosphate 0.030%
Sodium-chlor 0.020%
Repone K 0.020%
Sal epsom 0.080%
Manganous sulfate 0.003%
Ferrous sulfate 0.003%
Yeast powder 0.300%
Distilled water 98.00%;
(3) the silicate bacteria bacterium liquid of step (1), Jingyang streptomycete bacterium liquid are respectively entered mixing fermentation culture behind the seeding tank by 3% inoculum size, zymocyte liquid II; Above-mentioned seeding tank fermentation culture adopts following condition: 30 ℃ of temperature, 180～200 rev/mins of rotating speeds of cultivation, cultivation ventilation 1: 1 v: v/min; Incubation time 36 hours, 35 hours inspection bacterium; Above-mentioned seeding tank fermention medium is as follows:
Soybean cake powder 0.5%
Sucrose 0.5%
Yeast powder 0.5%
Ammonium sulfate 0.01%
Sal epsom 0.08%
Dipotassium hydrogen phosphate 0.1%
Sodium-chlor 0.01%
Lime carbonate 0.1%
Ferrous sulfate 0.001%
Distilled water 98.2%;
(4) the zymocyte liquid II of the zymocyte liquid I of step (2) and step (3) is respectively entered fermentor tank by 10%～20% inoculum size, the while mixed culture, 30 ℃ of culture temperature, cultivate 180～200 rev/mins of rotating speeds, cultivate 1: 1 v: the v/min that ventilates, incubation time 48 hours, the inspection bacterium goes out jar and obtains mixed fermentation liquid; It is as follows that above-mentioned four kinds of bacterium mix production jar fermention medium:
Starch 3%
Soybean cake powder 1%
Dipotassium hydrogen phosphate 0.03%
Ammonium sulfate 0.1%
Sal epsom 0.05%
Iron(ic) chloride 0.001%
Lime carbonate 0.01%
Yeast powder 0.02%
Distilled water 95.8%;
(2) water is joined in the above-mentioned mixed fermentation liquid by proportioning, mix evenly, get the aqueous solution of mixed fermentation liquid;
(3) with nitrogenous fertilizer, phosphate fertilizer, the potash fertilizer of solubility be by weight 1～2: 0.5～1: 1～2 mix the solubility azophoska, pulverize, cross 80 mesh sieves;
(4) with the aqueous solution of the mixed fermentation liquid of the solubility azophoska of step (three) and step (two) uniform mixing in proportion, all promptly get liquid composite microorganism fertilizer after the dissolving.
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| CN1600751A (en) * | 2004-10-26 | 2005-03-30 | 大连华夏生物产业有限公司 | Industrialization manufacturing method of double effect composite microbial manure |
| CN1654435A (en) * | 2004-12-25 | 2005-08-17 | 南通宏通生物科技有限公司 | Composite microbial manure and method for production thereof |
| CN1911870A (en) * | 2006-09-01 | 2007-02-14 | 山东省农业科学院高新技术研究中心 | Liquid composite microorganism fertilizer and its preparation method |
-
2008
- 2008-03-07 CN CN2008100147354A patent/CN101239847B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134408A (en) * | 1995-04-28 | 1996-10-30 | 王厚德 | Composite microbial fertilizer |
| CN1191854A (en) * | 1998-02-25 | 1998-09-02 | 北京乐泰然经济技术咨询有限责任公司 | Composite microbial fertilizer and its production process |
| CN1566038A (en) * | 2003-06-25 | 2005-01-19 | 中国科学院大连化学物理研究所 | Biological inorganic complex fertilizer |
| CN1587218A (en) * | 2004-07-09 | 2005-03-02 | 上海绿乐生物科技有限公司 | Composite microbial fertilizer containing humic acid decomposing bacteria |
| CN1600751A (en) * | 2004-10-26 | 2005-03-30 | 大连华夏生物产业有限公司 | Industrialization manufacturing method of double effect composite microbial manure |
| CN1654435A (en) * | 2004-12-25 | 2005-08-17 | 南通宏通生物科技有限公司 | Composite microbial manure and method for production thereof |
| CN1911870A (en) * | 2006-09-01 | 2007-02-14 | 山东省农业科学院高新技术研究中心 | Liquid composite microorganism fertilizer and its preparation method |
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| CN101239847A (en) | 2008-08-13 |
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