CN105060950A - Liquid composite microbial fertilizer and preparation method thereof - Google Patents

Liquid composite microbial fertilizer and preparation method thereof Download PDF

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Publication number
CN105060950A
CN105060950A CN201510168009.8A CN201510168009A CN105060950A CN 105060950 A CN105060950 A CN 105060950A CN 201510168009 A CN201510168009 A CN 201510168009A CN 105060950 A CN105060950 A CN 105060950A
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liquid
brown sugar
mixed bacteria
bacteria liquid
bacillus
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陈丽花
刘民胜
张家皓
顾伟民
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Shanghai Institute of Technology
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Shanghai Institute of Technology
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The present invention discloses a liquid composite microbial fertilizer and a preparation method thereof, wherein a mixed strain comprising Bacillus megaterium, Bacillus mucilaginosus and Bacillus licheniformis is adopted as a strain and a fermentation culture medium adopting cheap fish powder as a main raw material is used to carry out liquid fermentation so as to finally prepare the liquid composite microbial fertilizer, wherein the liquid composite microbial fertilizer has the high live bacterial content, integrates effects of phosphorus dissolving, potassium dissolving, plant pathogenic bacteria resistance, heavy metal adsorption and the like, and further has effects of effective plant growth promoting and agricultural product quality improving, and the detection results show that the live bacterial number, the total nutrient, the mixed bacteria number, the pH value and other indexes meet the index requirements in NY/T798-2004. According to the present invention, the liquid composite microbial fertilizer is the liquid live composite microbial product with effects of soil nutrition improving and agricultural product yield increasing or agricultural product quality improving.

Description

A kind of liquid composite microorganism fertilizer and preparation method thereof
Technical field
The present invention relates to a kind of liquid composite microorganism fertilizer and preparation method thereof, microorganism belonging to genus technical field of fertilizers.
Background technology
The bio-feritlizer of narrow sense, be also called Inoculant, bacterial manure or microbial fertilizer, refer to that specified microorganisms and nutritive substance are composited, can provide, keep or improve plant nutrition, improve agricultural output or improve the living microorganism goods of quality of agricultural product, itself containing nutritive element, can not replace chemical fertilizer.
Now commercially ubiquitous bio-feritlizer is mainly divided into three kinds.The first is dry pulverulence.Microorganism is wherein in dormant state, and shortcoming is that (1) living bacteria count is lower than liquid microbe fertilizer living bacteria count; (2) this kind of fertilizer needs cost long time microorganism from rhombspar or dissolution of calcium carbonate with discharge, could have a strong impact on its result of use.The second is liquid form.An advantage of this kind of fertilizer is that microbe density is much higher than pulverous, but microorganism is not be in dormant state, and vie each other between multiple bacterial classification living space and nutritive substance, some microbial growths will be suppressed, and therefore the quality guaranteed period is shorter.The third bio-feritlizer is in starch form, and most of available bio-feritlizer all exists with starch form in the market.Microorganism, in starch or gel, is in dormant state in certain hour, can starch and gel be consumed gradually before contact soil.But because starch is all a kind of comparatively general nutritive ingredient for any one microorganism (no matter being invasive organism or non-pathogenic microorganisms), therefore, the bio-feritlizer of starch form has higher contaminative compared with other microbial fertilizers, affects its result of use.The kind of the bio-feritlizer of current China independent intellectual property right and have very large gap between performance and developed country, country will spend every year and a large amount of remit import bio-feritlizer outward.
Phosphorus plays an important role to growth and development of plants, but plant but can not directly absorb the most of organophosphorus in soil and inorganic phosphorus.After using phosphate fertilizer, due to the fixed action of soil, most of phosphorus Ca2+, Fe3+, Al3+ in soil are combined, and form insoluble phosphate, thus occur the phenomenons such as fertilizer efficiency decline, utilization ratio reduction.According to statistics, the phosphate fertilizer that China adds up to apply farmland is had an appointment and 76% is accumulated in soil and can not be utilized.Long-term a large amount of chemical fertilizer that uses obviously can cause the adverse consequencess such as soil compaction, the decline of soil water-retaining power, grassland degeneration. and phytase, nuclease and Phosphoric acid esterase etc. that phosphate solubilizing microorganism produces accelerate the decomposition of the organic phosphorus compounds such as phytic acid, nucleic acid, phosphatide, the organic acid of its secretion can carry out chelating with inorganic phosphorus, indissoluble phosphorus is made to transform into available phosphorus, the release of phosphorus element can be promoted, improve Plant P Nutrition. therefore, using phosphate solubilizing microorganism as bio-feritlizer, there is the advantages such as production cost is low, effect good, free from environmental pollution.Bacillus megaterium ( bacillusmegatenum) be a kind of root system of plant growth-promoting bacterium, be also the conventional bacterial classification in microbial fertilizer.It is that B. mucilaginocus is urged in a kind of phosphorus decomposing, and the ADSORPTION STATE organophosphorus that cannot directly utilize plant in soil, inorganic phosphorus have obvious Decomposition, can increase soil fertility; Promote the volume increase of crop; Can reduce the organonitrogen in water body, phosphorus, the prussiate that can also to a certain degree degrade in trade effluent, plays a role to the eubiosis in water body environment.Fermentation culture Quito of current bacillus megaterium adopts Semen Maydis powder, bean cake powder as main raw material.
China's Potassic fertilizer resources wretched insufficiency, country needs the inorganic potash fertilizer of a large amount of foreign exchange imports every year.Colloid bacillus cereus ( bacillusmucilaginosus) be a kind of bacterium decomposing silicate minerals, more domestic scholars also call silicate bacteria it, can decompose the ecosystem mineral of the aluminum silicate such as feldspar, mica, make insoluble K, P, Si etc. in soil change solubility into and utilize for plant-growth.Various bioactivators Promoting plant growth can also be produced simultaneously.A large amount of field experiment shows to be that the biological potassium fertilizer of major ingredient shows good effect of increasing production to various farm crop on potassium deficient soil with colloid bacillus cereus.
Bacillus licheniformis (Bacilluslicheniformis)can multiple antimicrobial substance be produced, have good restraining effect to some phytopathogens.The fungal disease of Bacillus licheniformis to plant has good inhibition.As withered in black shank, graw mold of tomato, cotton and verticillium etc.Bacillus licheniformis all has good restraining effect to Aspergillus albicans, aspergillus niger, Paecilomyces varioti.Bacillus licheniformis is also applied to environmental pollution prevention and control research, and as degradation of pyrethroid pesticide residue, the dead thalline of Bacillus licheniformis can also adsorbing heavy metal cadmium (Cr 6+), plumbous (Pb 2+).
China is an aquatic products big country.A large amount of tankage can be produced in the fish products course of processing, mainly comprise head, tail, squama, fin, bone, skin and internal organ etc., account for seafood heavy about 50%, not only the smell is awful and livestock does not eat for these waste of aquatic, mostly directly abandon into environment, cause the serious wasting of resources and environmental pollution, a huge sum of money place to go often will be spent in recent years to put.Make full use of fish pomace, be not only the important research direction that processing of aquatic products utilizes, and be the inevitable requirement improving ecological benefits and social benefit.Therefore, how to deal carefully with and to utilize waste of aquatic, making it enter the benign cycle of the ecosystem, become society problem demanding prompt solution.Current in China, one of utilization ways of fish processing fent is through simply proposing oil, drying, being ground into fish meal.
The most cost of investment of preparation method of present microorganism fertilizer is high, and the technical problem such as it is single to there is bacterial classification, and product quality is low.
Summary of the invention
An object of the present invention is that the cost of investment existing in the preparation process in order to solve mentioned microorganism fertilizer is high, bacterial classification is single, technical problems such as the finished product viable count is low, preservation term is short and a kind of liquid composite microorganism fertilizer is provided, the bacterial classification that this liquid composite microorganism fertilizer has that preparation cost is low, preparation process adopts be three kinds to improving soil nutrient, improve agricultural output or improve the bacterium that quality of agricultural product has a difference in functionality and mix, the liquid composite microorganism fertilizer product viable count of final gained is low, preservation term is long.
Two of object of the present invention is to provide the preparation method of above-mentioned a kind of liquid composite microorganism fertilizer.
Technical scheme of the present invention
A preparation method for liquid composite microorganism fertilizer, is prepared by a method comprising the following steps and forms:
(1), the preparation of fermention medium
First, by fish meal, Semen Maydis powder, brown sugar removal of impurities pulverizing;
Then count by weight percentage, i.e. fish meal: Semen Maydis powder: brown sugar is the ratio of 60-70%:15-30%:10-15%, the fish meal after above-mentioned removal of impurities also being pulverized, Semen Maydis powder, brown sugar mix, and obtain compound;
Then by weight calculating, the compound of above-mentioned gained: water is the ratio of 4-5:100, is added to the water by the compound of above-mentioned gained, mixes, obtain fermention medium;
(2), actication of culture
By weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for the ratio of 1-3:1-3:0.5-1.5, by bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)the mixed bacteria liquid that bacillus megaterium, colloid bacillus cereus and Bacillus licheniformis are mixed to get joins and mass percent concentration is housed is in the shaking flask of the brown sugar water of 0.5-1.0%, after mixing, control temperature is 28-36 DEG C, on shaking table, cultivates 1.0-2.0h under rotating speed 160-280rpm condition, obtains the mixed bacteria liquid of activation;
Mass percent concentration is the consumption of the brown sugar water of 0.5-1.0%, calculates by volume, i.e. mixed bacteria liquid: mass percent concentration is the brown sugar water of 0.5-1.0% is 3-7:100;
(3), by the mixed bacteria liquid of the activation of step (2) gained, calculate in mass ratio, namely the mixed bacteria liquid activated: fermention medium is the ratio of 3-10:100, the mixed bacteria liquid of activation is inoculated in fermention medium, then control pH6.0-8.0, temperature 28-36 DEG C, rotating speed 120-280rpm carries out fermentation 15-25h, obtains liquid composite microorganism fertilizer.
The liquid composite microorganism fertilizer of above-mentioned gained meets indices requirement in NY/T798-2004, and with spread plate counting, final mattress number of living reaches 0.5 × 10 8-6.4 × 10 9cfu/mL, living bacteria count>=0.5 × 10 8cfu/mL, bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)in the viable count of each effective bacterium be no less than 0.01 × 10 8cfu/mL, total nutrient (N+P 2o 5+ K 2o)>=4.0%, miscellaneous bacteria rate≤15%, pH3.0-8.0, temperature is refrigerate at 4-10 DEG C, validity period at least three months.
Beneficial effect of the present invention
A kind of liquid composite microorganism fertilizer of the present invention, due to the mixed bacteria liquid be made up of bacillus megaterium, colloid bacillus cereus and Bacillus licheniformis for bacterial classification, the fermention medium being main raw material with the fish meal of cheapness carries out liquid fermenting and is prepared from, therefore there is preparation method simple, the features such as cost of investment is low.
Further, a kind of liquid composite microorganism fertilizer of the present invention, viable bacteria content is high, effectively can realize the effects such as phosphorus decomposing, potassium decomposing, anti-phytopathogen, Adsorption of Heavy Metals in one, therefore can effective Promoting plant growth, raising quality of agricultural product function.
Further, a kind of liquid composite microorganism fertilizer of the present invention, the indices such as viable count, total nutrient, miscellaneous bacteria number, pH value wherein all meets indices requirement in NY/T798-2004 after testing after testing.
In sum, the liquid composite microorganism fertilizer of gained of the present invention a kind ofly can improve soil nutrient, improves agricultural output or improve the liquid live body composite microbial Tetramune of quality of agricultural product.
Embodiment
Below by specific embodiment, the present invention is set forth further, but do not limit the present invention.
Bacillus megaterium used in various embodiments of the present invention ( bacillusmegatenum) deposit number: SIT4051, CGMCCNO.1.1870: be stored in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preservation organization address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode: 100101;
Colloid bacillus cereus ( bacillusmucilaginosus) deposit number: SIT4052, ACCCNO.10012: deposit number ACCCNO10012, be stored in Chinese agriculture Microbiological Culture Collection administrative center (AgriculturalCultureCollectionofChina, ACCC), preservation organization address: No.12 ,zhongguancun south street,Haidian District, Beijing Chinese agriculture Microbiological Culture Collection administrative center, postcode: 100081;
Bacillus licheniformis (Bacilluslicheniformis)deposit number: SIT4053, CGMCCNO.1.521: deposit number CGMCCNO1.521 is stored in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preservation organization address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode: 100101.
embodiment 1
A preparation method for liquid composite microorganism fertilizer, is prepared by a method comprising the following steps and forms:
(1), the preparation of fermention medium
First, by fish meal, Semen Maydis powder, brown sugar removal of impurities pulverizing;
Then count by weight percentage, i.e. fish meal: Semen Maydis powder: brown sugar is the ratio of 70%:15%:15%, the fish meal after above-mentioned removal of impurities also being pulverized, Semen Maydis powder, brown sugar mix, and obtain compound;
Then by weight calculating, the compound of above-mentioned gained: water is the ratio of 5:100, is added to the water by the compound of above-mentioned gained, mixes, obtain fermention medium;
(2), actication of culture
By weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for the ratio of 1:1:0.5, by bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)the mixed bacteria liquid 3mL be mixed to get joins and 100mL mass percent concentration is housed is in the 250mL Erlenmeyer flask of the brown sugar water of 0.5%, after mixing 28 DEG C, 1.0h cultivated by shaking table under rotating speed 160rpm, obtains the mixed bacteria liquid of activation;
Mass percent concentration is the consumption of the brown sugar water of 0.5%, calculates by volume, i.e. mixed bacteria liquid: mass percent concentration be 0.5% brown sugar water be 3:100;
(3), by the mixed bacteria liquid bacterial strain of the activation of step (2) gained, calculate in mass ratio, namely the mixed bacteria liquid activated: fermention medium is the ratio of 3:100, the mixed bacteria liquid of activation is inoculated in fermention medium, then control pH6.0, temperature 28 DEG C, rotating speed 120rpm carry out fermentation 15h, obtain liquid composite microorganism fertilizer.
With spread plate counting, in the liquid composite microorganism fertilizer of above-mentioned gained, final viable count reaches 0.5 × 10 8cfu/mL, bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)in the viable count of each effective bacterium be no less than 0.01 × 10 8cfu/mL.
Measure total nitrogen content according to GB/T8572 method, measure available phosphorus (P according to GB/T8573 method 2o 5) content, measure potassium (K according to GB/T8574 method 2o) content, result shows total nutrient (N+P 2o 5+ K 2o)>=4.0%.
Measure according to spread plate, miscellaneous bacteria rate≤15%.
Measure with neutral pH test paper, pH3.0-8.0, temperature is refrigerate at 4-10 DEG C, validity period at least three months.
In the liquid composite microorganism fertilizer of i.e. gained of the present invention, living bacteria count, total nutrient, miscellaneous bacteria rate, pH value indices all meet indices requirement in NY/T798-2004 after testing.
embodiment 2
A preparation method for liquid composite microorganism fertilizer, is prepared by a method comprising the following steps and forms:
(1), the preparation of fermention medium
First, by fish meal, Semen Maydis powder, brown sugar removal of impurities pulverizing;
Then count by weight percentage, i.e. fish meal: Semen Maydis powder: brown sugar is the ratio of 65%:20%:15%, the fish meal after above-mentioned removal of impurities also being pulverized, Semen Maydis powder, brown sugar mix, and obtain compound;
Then by weight calculating, the compound of above-mentioned gained: water is the ratio of 4:100, is added to the water by the compound of above-mentioned gained, mixes, obtain fermention medium;
(2), actication of culture
By weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for the ratio of 3:3:1.0, by bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)the 4ml mixed bacteria liquid be mixed to get joins and 100ml mass percent concentration is housed is in the brown sugar water mixing tank of 0.8%, after stirring and evenly mixing, then control temperature is that 32 DEG C of hybrid bacterial strains making to be made up of bacillus megaterium, colloid bacillus cereus and Bacillus licheniformis carry out activation 1.5h, obtains the mixed bacteria liquid of activation;
Mass percent concentration is the consumption of the brown sugar water of 0.8%, calculates by volume, i.e. mixed bacteria liquid: mass percent concentration be 0.8% brown sugar water be 4:100;
(3), by the mixed bacteria liquid of the activation of step (2) gained, calculate in mass ratio, namely the mixed bacteria liquid activated: fermention medium is the ratio of 10:100, the mixed bacteria liquid that 10ml activates is inoculated in the shaking flask of the 250ml that 100ml fermention medium is housed, then control pH7.0, temperature 32 DEG C, rotating speed 180rpm carry out fermentation 15h, obtain liquid composite microorganism fertilizer.
With spread plate counting, in the liquid composite microorganism fertilizer of above-mentioned gained, final viable count reaches 3.2 × 10 9cfu/mL, i.e. living bacteria count>=0.5 × 10 8cfu/mL, bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)in the viable count of each effective bacterium be no less than 0.01 × 10 8cfu/mL.
Measure total nitrogen content according to GB/T8572 method, measure available phosphorus (P according to GB/T8573 method 2o 5) content, measure potassium (K according to GB/T8574 method 2o) content, result shows total nutrient (N+P 2o 5+ K 2o)>=4.0%.
Measure according to spread plate, miscellaneous bacteria rate≤15%.
Measure with neutral pH test paper, pH3.0-8.0, temperature is refrigerate at 4-10 DEG C, validity period at least three months.
In the liquid composite microorganism fertilizer of i.e. gained of the present invention, living bacteria count, total nutrient, miscellaneous bacteria rate, pH value indices all meet indices requirement in NY/T798-2004 after testing.
embodiment 3
A preparation method for liquid composite microorganism fertilizer, is prepared by a method comprising the following steps and forms:
(1), the preparation of fermention medium
First, by fish meal, Semen Maydis powder, brown sugar removal of impurities pulverizing;
Then count by weight percentage, i.e. fish meal: Semen Maydis powder: brown sugar is the ratio of 60%:30%:10%, the fish meal after above-mentioned removal of impurities also being pulverized, Semen Maydis powder, brown sugar mix, and obtain compound;
Then by weight calculating, the compound of above-mentioned gained: water is the ratio of 5:100, is added to the water by the compound of above-mentioned gained, mixes, obtain fermention medium;
(2), actication of culture
By weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for the ratio of 3:3:1.5, by bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)the 7ml mixed bacteria liquid be mixed to get joins and 100ml mass percent concentration is housed is in the brown sugar water mixing tank of 1.0%, after stirring and evenly mixing, then control temperature is that 36 DEG C of hybrid bacterial strains making to be made up of bacillus megaterium, colloid bacillus cereus and Bacillus licheniformis carry out activation 0.5h, obtains the mixed bacteria liquid of activation;
Mass percent concentration is the consumption of the brown sugar water of 1.0%, calculates by volume, i.e. mixed bacteria liquid: mass percent concentration be 1.0% brown sugar water be 7:100;
(3), by the mixed bacteria liquid of the activation of step (3) gained, calculate in mass ratio, namely the mixed bacteria liquid activated: fermention medium is the ratio of 5:100, the mixed bacteria liquid that 5ml activates is inoculated in the shaking flask of the 250ml that 50ml fermention medium is housed, the mixed bacteria liquid of activation is inoculated in fermention medium, then control pH8.0, temperature 36 DEG C, rotating speed 280rpm carry out fermentation 25h, obtain liquid composite microorganism fertilizer.
With spread plate counting, in the liquid composite microorganism fertilizer of above-mentioned gained, final viable count reaches 6.4 × 10 9cfu/mL, i.e. living bacteria count>=0.5 × 10 8cfu/mL, bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)in the viable count of each effective bacterium be no less than 0.01 × 10 8cfu/mL.
Measure total nitrogen content according to GB/T8572 method, measure available phosphorus (P according to GB/T8573 method 2o 5) content, measure potassium (K according to GB/T8574 method 2o) content, result shows total nutrient (N+P 2o 5+ K 2o)>=4.0%.
Measure according to spread plate, miscellaneous bacteria rate≤15%.
Measure with neutral pH test paper, pH3.0-8.0, temperature is refrigerate at 4-10 DEG C, validity period at least three months.
In the liquid composite microorganism fertilizer of i.e. gained of the present invention, the indices such as living bacteria count, total nutrient, miscellaneous bacteria number, pH value all meet indices requirement in NY/T798-2004 after testing.
application Example
The liquid composite microorganism fertilizer of embodiment of the present invention 1-3 gained is used for the planting process of wild rice stem, the single microorganism fertilizer of the glad board of water paddy of producing with production base, national organic industry alliance East China Yancheng City god microbial strains Science and Technology Ltd. is for contrast: in planting process, carried out every 30-40 days respectively spraying (each 5 liters/mu after emerging, dilute with water 20 times uses afterwards), within first 15 days, stop using in results, compare with control group, the fertilizer of fertilizer 20-40% can be saved for every mu when the not underproduction, finally after testing, the liquid composite microorganism fertilizer of gained of the present invention can be drawn and contrast microbial fertilizer used, in phosphorus decomposing, potassium decomposing, anti-phytopathogen, Adsorption of Heavy Metals, the aspects such as preventing pollution have greater advantages.
In sum, a kind of liquid composite microorganism fertilizer of the present invention, with by bacillus megaterium, the mixed bacteria liquid of colloid bacillus cereus and Bacillus licheniformis composition is bacterial classification, the fermention medium being main raw material with the fish meal of cheapness carries out liquid fermenting and is prepared from, preparation method is simple, cost is low, in the liquid composite microbiological fertilizer of final gained, viable bacteria content is high, effectively can realize phosphorus decomposing, potassium decomposing, anti-phytopathogen, the effects such as Adsorption of Heavy Metals are in one, and the living bacteria count in gained composite microbiological fertilizer, total nutrient, miscellaneous bacteria number and pH value indices all meet indices requirement in NY/T798-2004 after testing.Therefore the liquid composite microbiological fertilizer of gained a kind ofly can improve soil nutrient, improves agricultural output or improve the liquid live body composite microbial Tetramune of quality of agricultural product.
Above said content be only the present invention conceive under basic explanation, and according to any equivalent transformation that technical scheme of the present invention is done, all should protection scope of the present invention be belonged to.

Claims (6)

1. a preparation method for liquid composite microorganism fertilizer, is characterized in that comprising the steps:
(1), the preparation of fermention medium
First, by fish meal, Semen Maydis powder, brown sugar removal of impurities pulverizing;
Then count by weight percentage, i.e. fish meal: Semen Maydis powder: brown sugar is the ratio of 60-70%:15-30%:10-15%, the fish meal after above-mentioned removal of impurities also being pulverized, Semen Maydis powder, brown sugar mix, and obtain compound;
Then by weight calculating, the compound of above-mentioned gained: water is the ratio of 4-5:100, is added to the water by the compound of above-mentioned gained, mixes, obtain fermention medium;
(2), actication of culture
By weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for the ratio of 1-3:1-3:0.5-1.5, by bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)the mixed bacteria liquid be mixed to get joins and mass percent concentration is housed is in the shaking flask of the brown sugar water of 0.5-1.0%, and after mixing, control temperature is 28-36 DEG C, on shaking table, cultivates 1.0-2.0h under the condition of rotating speed 160-280rpm, obtains the mixed bacteria liquid of activation;
Described bacillus megaterium ( bacillusmegatenum), deposit number: SIT4051, CGMCCNO.1.1870;
Described colloid bacillus cereus ( bacillusmucilaginosus), deposit number: SIT4052, ACCCNO.10012;
Described Bacillus licheniformis (Bacilluslicheniformis), deposit number: SIT4053, CGMCCNO.1.521;
Mass percent concentration is the consumption of the brown sugar water of 0.5-1.0%, calculates by volume, i.e. mixed bacteria liquid: mass percent concentration is the brown sugar water of 0.5-1.0% is 3-7:100;
(3), by the mixed bacteria liquid of the activation of step (2) gained, calculate in mass ratio, namely the mixed bacteria liquid activated: fermention medium is the ratio of 3-10:100, the mixed bacteria liquid of activation is inoculated in fermention medium, then control pH6.0 ~ pH8.0, temperature 28-36 DEG C, rotating speed 120-280rpm carries out fermentation 15h ~ 25h, obtains liquid composite microbiological fertilizer.
2. the preparation method of a kind of liquid composite microorganism fertilizer as claimed in claim 1, is characterized in that counting by weight percentage in step (1), i.e. fish meal: Semen Maydis powder: brown sugar is 70%:15%:15%;
By weight calculating, the compound of gained: water is 5:100;
In step (2) by weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for 1:1:0.5;
Calculate by volume, mass percent concentration is the consumption of the brown sugar water of 0.5%, by mixed bacteria liquid: mass percent concentration be 0.5% brown sugar water be 3:100;
In step (3), calculate in mass ratio, the mixed bacteria liquid namely activated: fermention medium is the ratio of 3:100, is inoculated in fermention medium by the mixed bacteria liquid of activation, then control pH6.0, temperature 28 DEG C, rotating speed 120rpm carry out fermentation 15h.
3. the preparation method of a kind of liquid composite microorganism fertilizer as claimed in claim 1, is characterized in that counting by weight percentage in step (1), i.e. fish meal: Semen Maydis powder: brown sugar is 65%:20%:15%;
By weight calculating, the compound of gained: water is 4:100;
In step (2) by weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for 3:3:1.0;
Calculate by volume, mass percent concentration is the consumption of the brown sugar water of 0.8%, by mixed bacteria liquid: mass percent concentration be 0.8% brown sugar water be 4:100;
In step (3), calculate in mass ratio, the mixed bacteria liquid namely activated: fermention medium is the ratio of 10:100, is inoculated in fermention medium by the mixed bacteria liquid of activation, then control pH7.0, temperature 32 DEG C, rotating speed 180rpm carry out fermentation 15h.
4. the preparation method of a kind of liquid composite microorganism fertilizer as claimed in claim 1, is characterized in that counting by weight percentage in step (1), i.e. fish meal: Semen Maydis powder: brown sugar is 60%:30%:10%;
By weight calculating, the compound of above-mentioned gained: water is 5:100;
In step (2) by weight calculate, namely bacillus megaterium ( bacillusmegatenum): colloid bacillus cereus ( bacillusmucilaginosus): Bacillus licheniformis (Bacilluslicheniformis)for 3:3:1.5;
By volume percentage calculation, mass percent concentration is the consumption of the brown sugar water of 1.0%, by mixed bacteria liquid: mass percent concentration be 1.0% brown sugar water be 7:100;
In step (3), calculate in mass ratio, the mixed bacteria liquid namely activated: fermention medium is the ratio of 5:100, is inoculated in fermention medium by the mixed bacteria liquid of activation, then control pH8.0, temperature 36 DEG C, rotating speed 280rpm carry out fermentation 25h, obtain liquid composite microorganism fertilizer.
5. as described in as arbitrary in claim 1-4, the preparation method of liquid composite microorganism fertilizer, is characterized in that the shaking flask liquid amount in step (2) is 40%.
6. a kind of liquid composite microorganism fertilizer obtained in preparation method as described in as arbitrary in claim 1-4, is characterized in that viable count reaches 0.5 × 10 8-6.4 × 10 9cfu/mL, living bacteria count>=0.5 × 10 8cfu/mL, bacillus megaterium ( bacillusmegatenum), colloid bacillus cereus ( bacillusmucilaginosus) and Bacillus licheniformis (Bacilluslicheniformis)in the viable count of each effective bacterium be no less than 0.01 × 10 8cfu/mL, total nutrient (N+P 2o 5+ K 2o)>=4.0%, miscellaneous bacteria rate≤15%, pH3.0-8.0, temperature is refrigerate at 4-10 DEG C, validity period at least three months.
CN201510168009.8A 2015-04-10 2015-04-10 Liquid composite microbial fertilizer and preparation method thereof Pending CN105060950A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108101646A (en) * 2017-12-29 2018-06-01 林立森 A kind of preparation method of flowers fruits and vegetables biological organic Liquid Fertilizer
CN108383596A (en) * 2018-04-03 2018-08-10 山东黎昊源生物工程有限公司 A kind of liquid microbe fertilizers and preparation method thereof instead of chemical fertilizer
CN108968767A (en) * 2018-08-15 2018-12-11 北京奥合兴科技有限公司 Microorganism padding closet system
CN113481119A (en) * 2021-06-25 2021-10-08 山东土木启生物科技有限公司 Compound microbial agent for improving phosphorus utilization rate

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1397521A (en) * 2002-07-26 2003-02-19 福建省尤溪县绿地生物制品有限公司 Liquid-state composite bacterial microbe fertilizer and its preparing process
CN101239847A (en) * 2008-03-07 2008-08-13 山东省农业科学院土壤肥料研究所 Liquid composite microbial fertilizer and its preparation method
CN102153402A (en) * 2010-12-15 2011-08-17 沈阳科丰牧业科技有限公司 Liquid complex microorganism bacterial manure and preparation method thereof
CN102391023A (en) * 2011-08-15 2012-03-28 北京华瑞康田生物科技有限公司 Production process for high-activity granular biologic organic fertilizer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1397521A (en) * 2002-07-26 2003-02-19 福建省尤溪县绿地生物制品有限公司 Liquid-state composite bacterial microbe fertilizer and its preparing process
CN101239847A (en) * 2008-03-07 2008-08-13 山东省农业科学院土壤肥料研究所 Liquid composite microbial fertilizer and its preparation method
CN102153402A (en) * 2010-12-15 2011-08-17 沈阳科丰牧业科技有限公司 Liquid complex microorganism bacterial manure and preparation method thereof
CN102391023A (en) * 2011-08-15 2012-03-28 北京华瑞康田生物科技有限公司 Production process for high-activity granular biologic organic fertilizer

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108101646A (en) * 2017-12-29 2018-06-01 林立森 A kind of preparation method of flowers fruits and vegetables biological organic Liquid Fertilizer
CN108383596A (en) * 2018-04-03 2018-08-10 山东黎昊源生物工程有限公司 A kind of liquid microbe fertilizers and preparation method thereof instead of chemical fertilizer
CN108968767A (en) * 2018-08-15 2018-12-11 北京奥合兴科技有限公司 Microorganism padding closet system
CN113481119A (en) * 2021-06-25 2021-10-08 山东土木启生物科技有限公司 Compound microbial agent for improving phosphorus utilization rate
CN113481119B (en) * 2021-06-25 2024-05-28 山东土木启生物科技有限公司 Composite microbial agent for improving phosphorus utilization rate

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