CN1397521A - Liquid-state composite bacterial microbe fertilizer and its preparing process - Google Patents
Liquid-state composite bacterial microbe fertilizer and its preparing process Download PDFInfo
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- CN1397521A CN1397521A CN 02138844 CN02138844A CN1397521A CN 1397521 A CN1397521 A CN 1397521A CN 02138844 CN02138844 CN 02138844 CN 02138844 A CN02138844 A CN 02138844A CN 1397521 A CN1397521 A CN 1397521A
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Abstract
A liquid-state compound microbial fertilizer is prepared from azotobacteria, bacillus megaterium, silicate bacteria and trace elements through physical and chemical induced mutation, mixing and diluting by water. Its advantages are high yield and quality of agricultural crops, and long quality guarantee period.
Description
Technical field:
The invention belongs to field of ecological agricultural, particularly a kind of liquid composite microbic bacterial fertilizer and production method thereof.
Background technology:
The microbial-bacterial fertilizer that current China bacterial manure factory produces, used bacterial classification is mainly the brown vinelandii in garden, huge gemma is planted bacterium, silicate bacteria etc., because the backwardness of production technique, selecting for use of bacterium culture medium or carrier is unreasonable, and its fixed nitrogen, phosphorus decomposing, ability of dissolving potassium are lower, and it is bigger to be subjected to the Effect of Environmental fluctuation, and adaptive capacity to environment is poor.Solid bacterial manure is when spraying use simultaneously, and method is comparatively loaded down with trivial details, peasant household's an urgent demand liquid-type bacterial manure listing.And current domestic liquid bacterial fertilizer adopts the direct can of fermented liquid, mineral oil front cover technology to produce more, the product maintenance phase is short, (be generally about 2 months, Ministry of Agriculture's standard is 6 months) the quality instability, only contain one or more effective bacterium in the product, only satisfy the requirement of crop to three big elements, all bacterial classifications are poor to adaptive capacity to environment simultaneously, when external environment changes, thalline fixed nitrogen, phosphorus decomposing, the ability of dissolving potassium problem such as can't play a role that descends.
Summary of the invention:
The objective of the invention is by means such as chemistry and physical mutagenesis, make viable bacteria keep longer, wide adaptability of phase, and take to select rational ligand complex mode to produce novel liquid composite microbic bacterial fertilizer various trace elements.
Technical solution of the present invention is achieved in that a kind of liquid composite microbic bacterial fertilizer, and the weight proportion of its three kinds of main active bacterial strains is as follows:
The brown vinelandii 50-60% in garden
Bacillus megaterium 20-30%
Silicate bacteria 20-25%
Its shared weight ratio of the composition of substratum is:
Sterilized water 90-94%
Corn steep liquor 0.1%
Brown sugar 1.0-4.5%
Sodium-chlor 0.05%
Lime carbonate 2-4%
Ethanol 1.0-1.2%
Acetic acid 1.0-1.2%
Vitamins C (V
C) 0.02%
Two butyl tetrahydroxy phenylmethylether (BHA) 50ppm that contract
The volume ratio scope of three kinds of useful bacterial strains and substratum is: 1: 2.8～1: 3.1.
The production method of liquid composite microbic bacterial fertilizer, its concrete making step is:
1) seed selection of bacterial strain:
A) collection of the brown vinelandii in garden, bacillus megaterium, three kinds of active bacterial strains of silicate bacteria is taken from the pure natural broad-leaf forest fully by beneath depth of humus layer;
B) each natural bacterial strain must be through purification, rejuvenation, chemomorphosis processing, UV treatment, plate isolation, and shake-flask culture, vitality test, mutagenic treatment etc. is several again takes turns circulation;
C) bacterial strain of Pei Yuing is a high osmotic pressure, in various, trace element selects rational complexing mode, can according to the Different Crop demand add various in, trace element, satisfy nutritional requirement comprehensively.
The concentrated solution of above-mentioned prefabricated brown vinelandii in garden, bacillus megaterium, silicate bacteria is quantitatively standby;
2) it is quantitatively standby to contain the sterile aqueous media of corn steep liquor, brown sugar, sodium-chlor, lime carbonate, ethanol, acetic acid, VC, BHA;
3) by above-mentioned 1) with 2) volume ratio be 1: 2.8～1: 3.1 two kinds of liquid mixing evenly and adjust PH=7.0～7.2;
4) small packages: the complex microorganism aqua that mixes is injected small package bag or Plastic Bottle by automatic packing machine and seal.
The technical essential that mentioned chemomorphosis is handled in the aforementioned production method is: (a) with bacterium liquid rare analyse to concentration be 180～2,200,000/ml; (b) uviolizing is 4～5 minutes, uses sun exposure again 5 minutes; (c) the basic guanidine concentration of treatment 1mg/ml of inferior pin, time 28-30 minute; (d) ethyl sulfate, concentration 1.0～1.2%, time 30-35 minute; (e) ethyl sulfate adds UV treatment, and UV treatment added ethyl sulfate (1%) and handled 15-20 minute after 5 minutes.
The product the key technical indexes of producing by said ratio is: viable count in the aqua 〉=8.0 hundred million/milliliter, PH=6.8-7.5.The thing of unpacking during use is poured in automiser spray and is converted that water is rare analyses 300 times and make foliage-spray or irritate root and use.
Advantage of the present invention mainly contains:
1) spawn activity greatly improves, one: the vigor of bacterial classification strengthens, and the bacterial strain through mutagenesis can make microorganism synthetic ammonia, organic acid pump the extracellular in good time, makes fixed nitrogen, phosphorus decomposing, the potassium decomposing of microorganism, and ability improves more than 20%; Its two, contain multiple amino acids in the novel bacterial, polysaccharide etc., these materials can promote the growth, resistance against diseases is strong; Its three, environmental compatibility improves, but deficient resistance, salt tolerant.
2) unit cost is low, and the liquid-type microbial inoculum adopts economic carrier compound, and its cost is basic and the solid type microbial inoculum is fair, but than freeze-dried formulation product price decline 20-30%.
3) function more comprehensively, application process is more flexible.Novel liquid bacterial agent also provides a large amount of promotion growth substances except that consolidating N, separate P, separating the K, as penicillin, indolylacetic acid, VITAMIN, amino acid etc., and compound various trace elements, can satisfy crop to nutrient demand.
4) compared with general foliage fertilizer low price 20-30%, and the quality raising, disease and pest reduces, and is the desirable fertilizer of producing green food or Organic food.
Embodiment:
Embodiment (one)
Use bacterium liquid 10ml of the present invention, rare analyse 300 times after, sprayed before the tea picking phase 15-20 days, sprayed 2-3 days, each 5-7 days at interval, can obtain the effect of increasing production of 11-13%.
Embodiment (two)
Use bacterium liquid 10ml of the present invention, dilute 300 times after, spray in blooming of fruit trees and the phase of bearing fruit and respectively spray 1 time, can obtain the effect of increasing production of 8-12%.
Claims (3)
1, a kind of liquid composite microbic bacterial fertilizer is characterized in that the weight proportion of three kinds of active bacterial strains is as follows:
The brown vinelandii 50-60% in garden
Bacillus megaterium 20-30%
Silicate bacteria 20-25%
Its shared weight ratio of the composition of substratum is:
Sterilized water 90-94%
Corn steep liquor 0.1%
Brown sugar 1.0-4.5%
Sodium-chlor 0.05%
Lime carbonate 2-4%
Ethanol 1.0-1.2%
Acetic acid 1.0-1.2%
Vitamins C (V
C) 0.02%
Two butyl tetrahydroxy phenylmethylether (BHA) 50ppm that contract
The volume ratio scope of three kinds of useful bacterial strains and substratum is: 1: 2.8～1: 3.1.
2, the method for the described liquid composite microbic bacterial fertilizer of production claim 1 is characterized in that producing concrete making step and is:
1) seed selection of bacterial strain: A) collection of the brown vinelandii in garden, bacillus megaterium, three kinds of active bacterial strains of silicate bacteria is taken from the pure natural broad-leaf forest fully by beneath depth of humus layer; B) each natural bacterial strain must be through purification, rejuvenation, chemomorphosis processing, UV treatment, plate isolation, and shake-flask culture, vitality test, mutagenic treatment etc. is several again takes turns circulation; C) bacterial strain of Pei Yuing is a high osmotic pressure, in various, trace element selects rational complexing mode, can according to the Different Crop demand add various in, trace element, satisfy nutritional requirement comprehensively.
The concentrated solution of above-mentioned prefabricated brown vinelandii in garden, bacillus megaterium, silicate bacteria is quantitatively standby;
2) it is quantitatively standby to contain the sterile aqueous media of corn steep liquor, brown sugar, sodium-chlor, lime carbonate, ethanol, acetic acid, VC, BHA;
3) by above-mentioned 1) with 2) volume ratio be 1: 2.8～1: 3.1 two kinds of liquid mixing evenly and adjust PH=7.0～7.2;
4) small packages: the complex microorganism aqua that mixes is injected small package bag or Plastic Bottle by automatic packing machine and seal.
3, the production method of liquid composite microbic bacterial fertilizer according to claim 2 is characterized in that the technical essential that chemomorphosis is handled is: (a) with bacterium liquid rare analyse to concentration be 180～2,200,000/ml; (b) uviolizing is 4～5 minutes, uses sun exposure again 5 minutes; (c) the basic guanidine concentration of treatment 1mg/ml of inferior pin, time 28-30 minute; (d) ethyl sulfate, concentration 1.0～1.2%, time 30-35 minute; (e) ethyl sulfate adds UV treatment, and UV treatment added ethyl sulfate (1%) and handled 15-20 minute after 5 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02138844 CN1397521A (en) | 2002-07-26 | 2002-07-26 | Liquid-state composite bacterial microbe fertilizer and its preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02138844 CN1397521A (en) | 2002-07-26 | 2002-07-26 | Liquid-state composite bacterial microbe fertilizer and its preparing process |
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| CN1397521A true CN1397521A (en) | 2003-02-19 |
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| CN 02138844 Pending CN1397521A (en) | 2002-07-26 | 2002-07-26 | Liquid-state composite bacterial microbe fertilizer and its preparing process |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372814C (en) * | 2005-06-20 | 2008-03-05 | 付锡臣 | Organic fertilizer prepared by using waste blackfungus culture medium and its preparation method and use |
| CN102153402A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Liquid complex microorganism bacterial manure and preparation method thereof |
| CN102391964A (en) * | 2011-11-12 | 2012-03-28 | 江苏天象生物科技有限公司 | Bacillus medium |
| CN102584453A (en) * | 2012-02-22 | 2012-07-18 | 中南林业科技大学 | Composite microorganism bacterial manure and preparation method thereof |
| CN104593303A (en) * | 2015-01-21 | 2015-05-06 | 威海金中元生物肥有限公司 | Liquid composite microbial agent and production method thereof |
| CN105060950A (en) * | 2015-04-10 | 2015-11-18 | 上海应用技术学院 | Liquid composite microbial fertilizer and preparation method thereof |
| WO2023210780A1 (en) * | 2022-04-28 | 2023-11-02 | 花王株式会社 | Plant growth promoter |
-
2002
- 2002-07-26 CN CN 02138844 patent/CN1397521A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372814C (en) * | 2005-06-20 | 2008-03-05 | 付锡臣 | Organic fertilizer prepared by using waste blackfungus culture medium and its preparation method and use |
| CN102153402A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Liquid complex microorganism bacterial manure and preparation method thereof |
| CN102391964A (en) * | 2011-11-12 | 2012-03-28 | 江苏天象生物科技有限公司 | Bacillus medium |
| CN102391964B (en) * | 2011-11-12 | 2013-03-13 | 江苏天象生物科技有限公司 | Bacillus medium |
| CN102584453A (en) * | 2012-02-22 | 2012-07-18 | 中南林业科技大学 | Composite microorganism bacterial manure and preparation method thereof |
| CN104593303A (en) * | 2015-01-21 | 2015-05-06 | 威海金中元生物肥有限公司 | Liquid composite microbial agent and production method thereof |
| CN104593303B (en) * | 2015-01-21 | 2018-02-13 | 威海金中元生物肥有限公司 | A kind of liquid complex micro organism fungicide and its production method |
| CN105060950A (en) * | 2015-04-10 | 2015-11-18 | 上海应用技术学院 | Liquid composite microbial fertilizer and preparation method thereof |
| WO2023210780A1 (en) * | 2022-04-28 | 2023-11-02 | 花王株式会社 | Plant growth promoter |
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