CN102391964B - Bacillus medium - Google Patents

Abstract

The invention provides a bacillus medium, which is added with components of red sugar, short bean sprout and the like and is suitable for the rapid growth of Bacillus pumilus and Paenibacillus polymyxa. The medium which can promote Bacillus pumilus and Paenibacillus polymyxa to rapidly enter the exponential growth phase allows the viable count of a fermentation broth to be averagely improved by more than 20%, the gemma proportion to be more than 90%, and the fermentation period to be shortened by 4-6h, so the product quality is greatly improved and the production cost is reduced.

Description

A kind of bacillus culture medium

Technical field

The present invention relates to a kind of microbiological culture media, specifically, relate to a kind of bacillus culture medium.

Background technology

For the industrialization deep liquid fermentation process of genus bacillus, it is on the low side that ubiquity fermentation level at present, and sporulation is inconsistent, the defectives such as unstable product quality.General take to adjust the processing condition such as temperature in the fermenting process, pressure, aeration-agitation more and improve the living bacteria count amount, or the brood cell of bacterial classification adopted pyroprocessing, make it in the industrial fermentation process, can form synchronously gemma, thereby improve quantity and the ratio of sporulation, shorten fermentation time, the very trouble but these method actually operatings are got up, and increased production cost.

Summary of the invention

The substratum that the purpose of this invention is to provide a kind of suitable bacillus pumilus, Paenibacillus polymyxa Fast Growth, quantity and the ratio of raising sporulation shorten fermentation time.

The scheme that the present invention takes is: the weight percent of each component is respectively among the present invention: corn steep liquor 0.7%, glucose 0.1%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, calcium carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2%(boil and get juice), water 96.17%.

The invention has the beneficial effects as follows that this substratum can impel bacillus pumilus, Paenibacillus polymyxa to enter fast logarithmic phase, the fermented liquid viable count on average can improve more than 20%, and the gemma ratio is greater than 90%.Can make fermentation period shorten 4-6 hour, thereby greatly improve quality product, reduce production cost.

Embodiment

Embodiment 1

Ferment with bacillus pumilus.With the following substratum fermentation of weight percentages of components.Corn steep liquor 0.7%, glucose 0.1%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, calcium carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2%(boil and get juice), water 96.17%.Adopt conventional zymotechnique in the fermentation system of 500L, to ferment,, fermented 23-24 hour up to 4,600,000,000 cfu/ml with the plate dilution assay method viable count, by microscopy gemma ratio greater than 90%.

Compare with the present invention and not add brown sugar, the viable count of the contrast substratum of short bean sprouts is 38.5cfu ∕ ml, and the 30 hours microscopy gemma ratios of fermenting only are 70-80%.

[0009]Embodiment 2

Ferment with Paenibacillus polymyxa.With the following substratum fermentation of weight percentages of components.Corn steep liquor 0.7%, glucose 0.1%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, calcium carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2%(boil and get juice), water 96.17%.Adopting conventional zymotechnique to ferment in the 500L fermentation system, is 1,200,000,000 cfu ∕ ml with the plate dilution assay method viable count, fermented 23-24 hour, by microscopy gemma ratio greater than 95%.

Compare with the present invention and not add brown sugar, the viable count of the contrast substratum of short bean sprouts is 9.6 hundred million cfu ∕ ml, and fermenting, microscopy gemma ratio is about 80% after 30 hours.

Claims (1)

1. bacillus culture medium, its component be by weight percentage:

Corn steep liquor 0.7%, glucose 0.1%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, calcium carbonate 0.1%, brown sugar 0.5%, bean sprout juice 2%, water 96.17%.