CN102391964A - Bacillus medium - Google Patents

Abstract

The invention provides a bacillus medium, which is added with components of red sugar, short bean sprout and the like and is suitable for the rapid growth of Bacillus pumilus and Paenibacillus polymyxa. The medium which can promote Bacillus pumilus and Paenibacillus polymyxa to rapidly enter the exponential growth phase allows the viable count of a fermentation broth to be averagely improved by more than 20%, the gemma proportion to be more than 90%, and the fermentation period to be shortened by 4-6h, so the product quality is greatly improved and the production cost is reduced.

Description

A kind of bacillus culture medium

Technical field

The present invention relates to a kind of microbiological culture media, specifically, relate to a kind of bacillus culture medium.

Background technology

For the industriallization deep liquid fermentation process of genus bacillus, it is on the low side that ubiquity fermentation level at present, and gemma forms inconsistent, defectives such as unstable product quality.General take to adjust processing condition such as temperature in the fermenting process, pressure, aeration-agitation more and improve the living bacteria count amount; Or the brood cell of bacterial classification adopted pyroprocessing; Make it in the industrial fermentation process, can form gemma synchronously, thereby improve quantity and the ratio that gemma forms, shorten fermentation time; The very trouble but these method actually operatings are got up, and increased production cost.

Summary of the invention

The purpose of this invention is to provide a kind of suitable bacillus pumilus, the Paenibacillus polymyxa substratum of growth fast, improve quantity and ratio that gemma forms, shorten fermentation time.

The scheme that the present invention takes is: the weight percent of each component is respectively among the present invention: steeping water 0.7%, glucose 0.1%, potassium hydrogenphosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, lime carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2% (boil and get juice), water 96.17%.

The invention has the beneficial effects as follows that this substratum can impel bacillus pumilus, Paenibacillus polymyxa to get into logarithmic phase fast, the fermented liquid viable count on average can improve more than 20%, and the gemma ratio is greater than 90%.Can make fermentation period shorten 4-6 hour, thereby improve quality product greatly, reduce production cost.

Embodiment

Embodiment 1

Ferment with bacillus pumilus.With the following substratum fermentation of weight percentages of components.Steeping water 0.7%, glucose 0.1%, potassium hydrogenphosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, lime carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2% (boil and get juice), water 96.17%.Adopt conventional zymotechnique in the fermentation system of 500L, to ferment, up to 4,600,000,000 cfu/ml, fermented 23-24 hour with the plate dilution assay method viable count, through microscopy gemma ratio greater than 90%.

Compare with the present invention and not add brown sugar, the viable count of the contrast substratum of short bean sprouts is 38.5cfu ∕ ml, and the 30 hours microscopy gemma ratios of fermenting are merely 70-80%.

 

[0009]Embodiment 2

Ferment with Paenibacillus polymyxa.With the following substratum fermentation of weight percentages of components.Steeping water 0.7%, glucose 0.1%, potassium hydrogenphosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, lime carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2% (boil and get juice), water 96.17%.Adopt conventional zymotechnique in the 500L fermentation system, to ferment, using the plate dilution assay method viable count is 1,200,000,000 cfu ∕ ml, fermented 23-24 hour, through microscopy gemma ratio greater than 95%.

Compare with the present invention and not add brown sugar, the viable count of the contrast substratum of short bean sprouts is 9.6 hundred million cfu ∕ ml, and fermenting, microscopy gemma ratio is about 80% after 30 hours.

Claims (2)

1. bacillus culture medium, its component be by weight percentage:

Steeping water 0.7%, glucose 0.1%, potassium hydrogenphosphate 0.2%, ammonium sulfate 0.03%, sal epsom 0.05%, yeast extract paste 0.05%, peptone 0.1%, lime carbonate 0.1%, brown sugar 0.5%, short bean sprouts 2%.

2. a kind of bacillus culture medium as claimed in claim 1 is characterized in that: the short-and-medium bean sprouts of this substratum is boiled and is got juice.