CN102079674A - Microorganism organic fertilizer and preparation method thereof - Google Patents
Microorganism organic fertilizer and preparation method thereof Download PDFInfo
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- CN102079674A CN102079674A CN2010105588435A CN201010558843A CN102079674A CN 102079674 A CN102079674 A CN 102079674A CN 2010105588435 A CN2010105588435 A CN 2010105588435A CN 201010558843 A CN201010558843 A CN 201010558843A CN 102079674 A CN102079674 A CN 102079674A
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Abstract
The invention discloses a microorganism organic fertilizer, which consists of straw, flour, lime, potassium dihydrogen phosphate, water and liquid mixed strain (NEM), wherein the weight percentages of the straw, the flour, the lime, the potassium dihydrogen phosphate and the water are as follows: 65 to 75% by weight of straw, 12 to 15% by weight of flour, 1% by weight of lime, 0.5% by weight of potassium dihydrogen phosphate and 13.5 to 21.5% by weight of water, and the addition amount of the liquid mixed strain (NEM) accounts for 5 to 10% by weight of the total weight of the straw, the flour, the lime, the potassium dihydrogen phosphate and the water. According to the invention, plenty of phosphatase, protease, polypeptide amino and other substances are generated in mixed fermentation, the utilization rate of fertilizers is enhanced, the growth of plant root is simultaneously promoted so that the plant root absorbs more nutrients, the growing plant feeds a large number of nutrients back to soil through the root owing to the favorable development of the root, thereby promoting the microbial proliferation in soil, therefore, such a sound cycle facilitates soil improvement and achieves the effect of soil repair; furthermore, the microorganism organic fertilizer is simple in production process, low in cost and convenient for popularization and application.
Description
Technical field
The present invention relates to a kind of microbial organic fertilizer, relate to utilize microbial inoculant soil to carry out the technology of soil remediation specifically, promptly utilizing stalk is main carbon source, carries out multiple bacteria compound fermentation, produces high reactivity fertilizer and preparation method thereof.
Background technology
At present, in the agricultural planting of China, use chemical fertilizer in a large number, not only caused environmental pollution, and cause a large amount of energy consumptions.In utilizing the ecological agriculture of low-carbon economy, live by improving soil enzyme to the soil inoculation probiotics, improve the fertilizer utilization ratio, reduce the usage quantity of chemical fertilizer, be the important measures that current soil is repaired.It is active high that liquid bacterial agent has, and wieldy characteristics are that rehabilitating soil improves one of effective for repairing agent of soil organisms fertility.But its cost height, the popularization of this technology that has been difficult for the serious obstruction of problems such as preservation.A kind of Cheap highly effective be the microbial organic fertilizer of main material production with the steam puffed stalk, will solve this difficult problem.
Microbial organic fertilizer is that the vital movement with microorganism causes crop to obtain a kind of goods of specific fertilizer effect, is to use a kind of of fertilizer in the agriculture production.More Zao than China abroad to the research and the application of microbial organic fertilizer.Its principal item is the root nodule bacterium seed dressings.China's microbial organic fertilizer development is rapider over more than 10 year, but that root nodule bacterium are used is less, the fertilizer of other kind such as fixed nitrogen, separate big gun, potassium decomposing, gemma class preparation use more, batch production production mostly.The production of microbial organic fertilizer is new and high technology, and total sees, the production of microbial organic fertilizer is not only fermenting process, and the effective combination from strain improvement, rejuvenation, renewal even gene recombination and different strain all needs hi-tech.Comprise that also fermentation equipment is supporting, technology is reasonable, the stable and microorganism of production performance enters deciding behind the soil and grows ecological studies such as competition.To understand the relation between microorganism and crop varieties, soil fertility, the soil type in a word, reach the purpose of reasonable application of organic fertilizers.The formulation of microbial organic fertilizer has 2 kinds of liquid and solids, no matter any formulation all will have provide protection to microorganism, makes it as far as possible existence for a long time, successfully enters the soil breeding.Be protection Agro-ecology, nutrient fortified food safety, western countries begin to use more the microbial organic fertilizer that separates beneficial microorganism from soil, make after the seed selection enlarged culturing in agriculture production in recent years.Agricultural experts generally believe; compare with traditional fertilizer; microbial organic fertilizer will be realized the Sustainable development of agriculture production having clear superiority aspect ecological protection, raising crop yield and the raising fertilizer utilization ratio, and China is necessary to greatly develop and promote microbial organic fertilizer.For guaranteeing that grain reaches the target of high yield, each generalized grain producing region of China generally relies on " chemical fertilizer " and " agricultural chemicals " two big " magic weapon " now, and such production model b referred to as " petrochemical industry agricultural ".Until now, " petrochemical industry agricultural " improving grain yield, solves China grain security aspect and brought into play crucial effect from founding the state.Yet along with chemical fertilizer and agricultural chemicals dispensing amount increase year by year, in recent years, we also begin to experience the negative impact that it brings agriculture production when enjoyment " petrochemical industry agricultural " brings benefit.If China continues by adding the target that large chemical fertilizer and agricultural chemicals input reach stable high yield, agroecological environment will be absorbed in said in the world " vicious circle of petrochemical industry agricultural " worse and worse so, finally is unfavorable for the stable of grain-production.How to jump out seeking on " petrochemical industry agricultural vicious circle " this problem, American-European developed country actively researches and develops and applies microbial organic fertilizer in agriculture production, when realizing " low input, high production, Sustainable development " target, improve quality of agricultural product again, repaired agroecological environment.As the nuisanceless organic fertilizer of indirect, use microbial organic fertilizer not only can bring into play the advantage of petrochemical industry agricultural, but also can overcome the drawback of petrochemical industry agricultural.Compare with traditional fertilizer, microbial organic fertilizer all has advantage on ecological protection, raising crop quality and raising fertilizer utilization ratio.
Summary of the invention
The present invention aims to provide a kind of microbial organic fertilizer and preparation method, stalk is formed the substratum and the carrier of functional flora, after microbial inoculum is manured into soil, come activating soil with the stalk vector for the plurality of movable center again, overcome the influence of indigenous family flora in the native soil, realized the purpose of rehabilitating soil; After the stalk detoxification, handle by microbial fermentation again, the toxin of stalk is thoroughly degraded, the fertilizer that makes can fertilizer for replacing chemical, has promotes growth, disease-resistant function simultaneously.
For achieving the above object, the technical scheme that adopts is: a kind of microbial organic fertilizer, form by stalk, flour, lime, potassium primary phosphate, water and liquid mixing bacterial classification NEM, the weight ratio of stalk, flour, lime, potassium primary phosphate and water is: stalk 65-70%, flour 12-15%, lime 1%, potassium primary phosphate 0.5%, water 13.5-21.5%, the add-on of liquid mixing bacterial classification NEM: the 5-10% that is stalk, flour, lime, potassium primary phosphate and water gross weight by weight.
Described liquid mixing bacterial classification NEM is made after fermentation culture by subtilis, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, photosynthetic bacteria and pantoea agglomerans; The weight ratio of each bacterial classification is: subtilis 7-9%, Candida utilis 7-9%, bacillus megaterium 7-9%, bacillusmusilaginosiengineering 7-9%, plant lactobacillus 15%, photosynthetic bacteria 15%, pantoea agglomerans 34-42%.
Substratum during seed tank culture is: brown sugar 5-8%, and dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, peptone 0.5%, surplus is a water, and initial pH value is transferred to 7.0.
The present invention, the cultural method of liquid mixing bacterial classification NEM is:
One, choose known bacterial classification: subtilis, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, photosynthetic bacteria and pantoea agglomerans, standby;
Two, the shaking culture of each bacterial classification:
A, the subtilis after will purifying are inserted triangular flask, shaking culture 24 hours from the inclined-plane;
B, the Candida utilis after will purifying insert triangular flask, shaking culture 48 hours from the inclined-plane;
C, the bacillus megaterium after will purifying are inserted triangular flask, shaking culture 24 hours from the inclined-plane;
D, the bacillusmusilaginosiengineering after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
E, the plant lactobacillus after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
F, the photosynthetic bacteria after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
G, the pantoea agglomerans after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane.
Three, with above-mentioned cultured each bacterial classification, get subtilis 7-9%, Candida utilis 7-9%, bacillus megaterium 7-9%, bacillusmusilaginosiengineering 7-9%, plant lactobacillus 15%, photosynthetic bacteria 15%, pantoea agglomerans 34-42% by weight, mix, form the bacterial classification mixed liquor I, after be linked into the ferment-seeded jar by weight;
Four, the substratum of seeding tank is: brown sugar 6%, and dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, peptone 0.5%, surplus is a water, and initial pH value is transferred to 7.0, temperature transfers to 121 ℃, sterilizes 30 minutes; During postcooling to 30 ℃, the gross weight of pressing material in the seeding tank inserts 4% bacterial classification mixed liquor I; Anaerobically fermenting 48-72 hour, rotating speed 180r/min, 32 ℃ of temperature when the pH value reaches 4.8, are fermentation termination when viable count reaches 1,500,000,000/ml, make liquid mixing bacterial classification NEM.
The present invention, described stalk is: a kind of in cornstalk, sorghum stalk, beans bar or the straw, or two or more combinations.
The present invention, described flour is: a kind of in Semen Maydis powder or the bean powder.
The best proportioning of stalk of the present invention is: be made up of cornstalk, sorghum stalk, beans bar and straw, the weight percent of each material is: cornstalk 62-65%, sorghum stalk 10%, beans bar 10%, straw 15-18%.
The best proportioning of flour of the present invention is: be made up of Semen Maydis powder and bean powder, the weight ratio of each material is: Semen Maydis powder 50-70%, bean powder 30-50%.
A kind of preparation method of microbial organic fertilizer, step is as follows:
Step 1, earlier straw powder is broken into the particle of 5-7mm, standby;
Step 2, get stalk 65-70%, flour 12-15%, lime 1%, potassium primary phosphate 0.5% by weight, mix and stirred 2 minutes, the back adds the water of 13.5-21.5%, mixes and stirs 3-5 minute;
Step 3, the compound that step 2 is made are sent in the pressurized vessel, send into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, and temperature rises to 160-180 ℃, sterilized 10 minutes, after material is sent into fermentation cabin;
After step 4, material were sent into fermentation cabin, by the 5-10% access liquid mixing bacterial classification NEM of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 72-96 hour, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
The pressurized vessel that the present invention adopts is the pressure-pot with pressurize of routine, pressurized vessel that preferably can the abrupt release material, as: the container of similar making puffed rice.
The liquid spawn that the present invention adopts, can also form by subtilis, Bacillus licheniformis, bacillus thuringiensis, actinomycetes, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, pantoea agglomerans and photosynthetic bacteria, the weight ratio of each bacterial classification is: subtilis 10%, Bacillus licheniformis 10%, bacillus thuringiensis 5%, actinomycetes 5%, Candida utilis 10%, bacillus megaterium 10%, bacillusmusilaginosiengineering 10%, plant lactobacillus 10%, pantoea agglomerans 20%, photosynthetic bacteria 10%.
The bacterial classification that the present invention adopts all is known commercially available bacterial classification, can pass through China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), and the China typical case cultivates preservation center (CCTCC) introduction and buys.Also can buy, be applied in the industrialization by screening in the Chinese Academy of Agricultural Sciences's microbial strains preservation center introduction.
Among the preparation method of the present invention, adopted cold water formula plane heating panel in the fermentation cabin, its frame mode is: the bottom of heating panel is a concave shape groove, active cold water is arranged in the groove, groove face is provided with the flat panel that can hold material, when material is put in when dispelling the heat on the heating panel, dispel the heat with cold water in the bottom, dispels the heat with cold wind in top.
The microbial organic fertilizer that the present invention produces has more ecological dominance than chemical fertilizer.The microbial organic fertilizer usage quantity is few, and itself is nontoxic, does not have problem of environment pollution caused, and the use of chemical fertilizer has then caused serious edatope to pollute.For example, because a large amount of nitrogen fertilizer that uses, groundwater azotate content rises.In addition, ammonia-state nitrogen in the chemical fertilizer and phosphoric acid class material, because the drip washing and the microbial process of rainwater, many losses have been advanced in the rivers and lakes, cause the eutrophication of rivers and lakes.In addition, microbial organic fertilizer can also improve the crop rhizosphere niche, forms useful collarium border at crop rhizosphere, and the opposing germ is improved the crop disease-resistant ability.Microbial organic fertilizer more has superiority on raising crop yield and quality than chemical fertilizer.Microbial organic fertilizer can increase the profitable strain in the soil, the nutriment that is difficult to utilize in the help crop utilization soil, thus realize increasing both production and income.Find in the vegetable field experiment on ground such as Shandong, Henan that according to fertilizer inspection center of the Ministry of Agriculture microbial organic fertilizer can make agricultural-food volume increase 12%-20%.In addition, in recent years, be the increase of " food origin disease " of representative with cancer and subhealth state phenomenon, exceeding standard with environmental pollution and food drug residue has direct relation, and microbial organic fertilizer can play the effect that reduces pesticide residue.Microorganism can be improved the utilization ratio of fertilizer.The average utilization of traditional fertilizer only has 20%-30%, and the utilization ratio of phosphate fertilizer is 10%-20%, and the utilization ratio of potash fertilizer is 40%, and the utilization ratio of microbial organic fertilizer will be higher than these numerals.For example, the fixed nitrogen almost can all be absorbed by the beans tannin plant in the root nodule bacterium.Popularize the microorganism chemical fertilizer and can bring the tremendous economic advantage, for cost is saved in grain-production.At present, 1,200,000,000 tons of the usage quantity average out to of the annual chemical fertilizer of China, if microbial organic fertilizer output reaches 3% of fertilizer amount, usable floor area reaches 100,000,000 mu, can reduce by 120,000-500,000 tons of fertilizer amounts every year, produces more food 5,000,000,000-10,000,000,000 kilograms.In addition, microbial organic fertilizer has bigger product to throw ratio, and the fund of every mu of use only is the 60%-70% of chemical fertilizer, can significantly save cost for the peasant.
The present invention is on a large amount of careful market study bases, main according to State Statistics Bureau, State General Administration of Customs, national Department of Commerce, National Development and Reform Committee, national fertilizer Professional Committee, to the supply and demand of China's microbial organic fertilizer industry, the production problem of importing and exporting existence in situation, consumption and market, competition and the development and the defective of production technique, national conditions in conjunction with China, at the characteristics of microbial organic fertilizer, solve the method that stalk is produced microbial organic fertilizer.
The present invention also has following beneficial effect:
Select the functional bacterium mixed fermentation in the soil for use, make function yeast more stable its function of performance in mixed fermentation, and in mixed fermentation, produce a large amount of Phosphoric acid esterases, proteolytic enzyme, class materials such as polypeptide amino, promoted fertilizer utilization ratio, promoted the growth of roots of plants simultaneously, make it absorb more nutrient, because root development is good, growing plants feeds back to a large amount of nutriment of soil by root, has promoted the breeding of soil microorganisms again, this benign cycle, promoted soil improvement, reached the effect of rehabilitating soil, production technique is simple, cost is low, and is easy to utilize.
Stalk is quick-fried through vapour, after the sterilization, detoxification fully, handle by microbial fermentation again, the fertilizer of producing contains a large amount of microorganism isoreactivity materials, not only can fertilizer for replacing chemical, also has promotes growth simultaneously, disease-resistant function, microbial organic fertilizer can make the agricultural-food volume increase more than 20%, and the fixed nitrogen almost can all be utilized by plant absorbing in the root nodule bacterium, can increase the profitable strain in the soil, the nutriment that is difficult to utilize in the help crop utilization soil, thereby the realization increasing both production and income, the microbial organic fertilizer usage quantity is few, and itself is nontoxic, do not have problem of environment pollution caused, the use of chemical fertilizer has then caused serious edatope to pollute; Can overcome with cancer and subhealth state phenomenon is the increase of " food origin disease " of representative, reduces the problem that environmental pollution and food drug residue exceed standard, and microbial organic fertilizer can play the effect that reduces pesticide residue.
Embodiment
A kind of microbial organic fertilizer, form by stalk, flour, lime, potassium primary phosphate, water and liquid mixing bacterial classification NEM, the weight ratio of stalk, flour, lime, potassium primary phosphate and water is: stalk 65-70%, flour 12-15%, lime 1%, potassium primary phosphate 0.5%, water 13.5-21.5%, the add-on of liquid mixing bacterial classification NEM: the 5-10% that is stalk, flour, lime, potassium primary phosphate and water gross weight by weight.
Described liquid mixing bacterial classification NEM is made after the ferment-seeded jar is cultivated by subtilis, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, photosynthetic bacteria and pantoea agglomerans.
The weight ratio that each bacterial classification adds is: subtilis 7-9%, Candida utilis 7-9%, bacillus megaterium 7-9%, bacillusmusilaginosiengineering 7-9%, plant lactobacillus 15%, photosynthetic bacteria 15%, pantoea agglomerans 34-42%.
Substratum during seed tank culture is: brown sugar 5-8%, and dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, peptone 0.5%, surplus is a water, and initial pH value is transferred to 7.0.
The cultural method of liquid mixing bacterial classification NEM is:
One, choose known bacterial classification: subtilis, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, photosynthetic bacteria and pantoea agglomerans, standby;
Two, with above-mentioned each bacterial classification shaking culture
A, the subtilis after will purifying are inserted triangular flask, shaking culture 24 hours from the inclined-plane;
B, the Candida utilis after will purifying insert triangular flask, shaking culture 48 hours from the inclined-plane;
C, the bacillus megaterium after will purifying are inserted triangular flask, shaking culture 24 hours from the inclined-plane;
D, the bacillusmusilaginosiengineering after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
E, the plant lactobacillus after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
F, the photosynthetic bacteria after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
G, the pantoea agglomerans after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
Three, with above-mentioned cultured each bacterial classification, get subtilis 7-9%, Candida utilis 7-9%, bacillus megaterium 7-9%, bacillusmusilaginosiengineering 7-9%, plant lactobacillus 15%, photosynthetic bacteria 15%, pantoea agglomerans 34-42% by weight, mix, form the bacterial classification mixed liquor I, after be linked into the ferment-seeded jar by weight;
Four, the substratum of seeding tank is: brown sugar 6%, dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, peptone 0.5%, surplus is a water, and initial pH value is transferred to 7.0, under 121 ℃ condition, sterilized 30 minutes, and during postcooling to 30 ℃, pressed 4% of seeding tank material gross weight and insert the bacterial classification mixed liquor I; Anaerobically fermenting 48-72 hour, rotating speed 180r/mi n, 32 ℃ of temperature when the pH value reaches 4.8, are fermentation termination when viable count reaches 1,500,000,000/ml, make liquid mixing bacterial classification NEM.
Described stalk is: a kind of in cornstalk, sorghum stalk, beans bar or the straw, or two or more combinations.
Described flour is: a kind of in Semen Maydis powder or the bean powder.
The best proportioning of described stalk is: be made up of cornstalk, sorghum stalk, beans bar and straw, the weight percent of each material is: cornstalk 62-65%, sorghum stalk 10%, beans bar 10%, straw 15-18%.
The best proportioning of described flour is: be made up of Semen Maydis powder and bean powder, the weight ratio of each material is: Semen Maydis powder 50-70%, bean powder 30-50%.
A kind of preparation method of microbial organic fertilizer, step is as follows:
Step 1, earlier straw powder is broken into the particle of 5-7mm, standby;
Step 2, get stalk 65-70%, flour 12-15%, lime 1%, potassium primary phosphate 0.5% by weight, mix and stirred 2 minutes, the back adds the water of 13.5-21.5%, mixes and stirs 3-5 minute;
Step 3, the compound that step 2 is made are sent in the pressurized vessel, send into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, and temperature rises to 160-180 ℃, sterilized 10 minutes, after material is sent into fermentation cabin;
After step 4, material were sent into fermentation cabin, by the 5-10% access liquid mixing bacterial classification NEM of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 72-96 hour, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
Embodiment 1
Get cornstalk 65%, sorghum stalk 10%, beans bar 10%, straw 15% after the pulverizing by weight, mix, form the stalk compound, standby;
Get Semen Maydis powder 70%, bean powder 30% by weight, mix, form the flour compound, standby;
Get stalk compound 65%, flour compound 12%, lime 1%, potassium primary phosphate 0.5% by weight, mix and stirred 2 minutes, the back adds 21.5% water, mixes and stirs 3-5 minute, makes the fermentation compound;
The fermentation compound of making is sent in the pressurized vessel, sent into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, temperature rises to 180 ℃, sterilized 10 minutes, after the compound that will ferment send into fermentation cabin;
After the compound that will ferment is sent into fermentation cabin, insert liquid mixing bacterial classification NEM by 10% of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 72 hours, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
Embodiment 2
Get cornstalk 62%, sorghum stalk 10%, beans bar 10%, straw 18% after the pulverizing by weight, mix, form the stalk compound, standby;
Get Semen Maydis powder 50%, bean powder 50% by weight, mix, form the flour compound, standby;
Get stalk compound 70%, flour compound 15%, lime 1%, potassium primary phosphate 0.5% by weight,, to mix and stirred 2 minutes, the back adds 13.5% water, mixes and stirs 3-5 minute, makes the fermentation compound;
The fermentation compound of making is sent in the pressurized vessel, sent into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, temperature rises to 160 ℃, sterilized 10 minutes, after the compound that will ferment send into fermentation cabin;
After the compound that will ferment is sent into fermentation cabin, insert liquid mixing bacterial classification NEM by 5% of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 96 hours, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
Embodiment 3
Get cornstalk 65%, Semen Maydis powder 12%, lime 1%, potassium primary phosphate 0.5% by weight, mix and stirred 2 minutes, the back adds 21.5% water, mixes and stirs 3-5 minute, makes the fermentation compound;
The fermentation compound of making is sent in the pressurized vessel, sent into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, temperature rises to 180 ℃, sterilized 10 minutes, after the compound that will ferment send into fermentation cabin;
After the fermentation compound is sent into fermentation cabin, insert liquid mixing bacterial classification NEM by 6% of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 72 hours, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
Embodiment 4
Get disintegrating Chinese sorghum stalk 80%, straw 20% by weight, mix the back and form the stalk compound, standby;
Get stalk compound 65%, bean powder 12%, lime 1%, potassium primary phosphate 0.5% by weight, mix and stirred 2 minutes, the back adds 21.5% water, mixes and stirs 3-5 minute, makes the fermentation compound;
The fermentation compound of making is sent in the pressurized vessel, sent into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, temperature rises to 180 ℃, sterilized 10 minutes, after the compound that will ferment send into fermentation cabin;
After the fermentation compound is sent into fermentation cabin, insert liquid mixing bacterial classification NEM by 8% of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 72 hours, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
The result of use of trial employment period:
One: 2008 year in June ,-2010 of example on probation, to try out in the Luolong District Li Lou of Luoyang City, Henan Province village, farm crop are: wheat; Area: 10 mu; Test effect: reduce fertilizer amount 50%, volume increase 30%.
Example on probation two: 2009 years is tried out in the Zhi of Pingdingshan City, Henan Province sun town XiZhi Cun, and farm crop are: corn; Area: 10 mu; Test effect: reduce fertilizer amount 50%, volume increase 10%.
Example on probation three: 2009 years is tried out in Yichuan County, Luoyang City, Henan Province, and farm crop are: paddy rice; Area: 20 mu; Test effect: reduce fertilizer amount 50%, volume increase 30%.
Claims (4)
1. microbial organic fertilizer, form by stalk, flour, lime, potassium primary phosphate, water and liquid mixing bacterial classification NEM, the weight ratio of stalk, flour, lime, potassium primary phosphate and water is: stalk 65-70%, flour 12-15%, lime 1%, potassium primary phosphate 0.5%, water 13.5-21.5%, the add-on of liquid mixing bacterial classification NEM: the 5-10% that is stalk, flour, lime, potassium primary phosphate and water gross weight by weight;
Described stalk is: a kind of in cornstalk, sorghum stalk, beans bar or the straw, or two or more combinations;
Described flour is: a kind of in Semen Maydis powder or the bean powder;
Described liquid mixing bacterial classification NEM is made after fermentation culture by subtilis, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, photosynthetic bacteria and pantoea agglomerans; The weight ratio of each bacterial classification is: subtilis 7-9%, Candida utilis 7-9%, bacillus megaterium 7-9%, bacillusmusilaginosiengineering 7-9%, plant lactobacillus 15%, photosynthetic bacteria 15%, pantoea agglomerans 34-42%;
The cultural method of described liquid mixing bacterial classification NEM is:
One, choose known bacterial classification: subtilis, Candida utilis, bacillus megaterium, bacillusmusilaginosiengineering, plant lactobacillus, photosynthetic bacteria and pantoea agglomerans, standby;
Two, with each bacterial classification shaking culture:
A, the subtilis after will purifying are inserted triangular flask, shaking culture 24 hours from the inclined-plane;
B, the Candida utilis after will purifying insert triangular flask, shaking culture 48 hours from the inclined-plane;
C, the bacillus megaterium after will purifying are inserted triangular flask, shaking culture 24 hours from the inclined-plane;
D, the bacillusmusilaginosiengineering after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
E, the plant lactobacillus after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
F, the photosynthetic bacteria after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
G, the pantoea agglomerans after will purifying insert triangular flask, shaking culture 24 hours from the inclined-plane;
Three, with above-mentioned cultured each bacterial classification, get subtilis 7-9%, Candida utilis 7-9%, bacillus megaterium 7-9%, bacillusmusilaginosiengineering 7-9%, plant lactobacillus 15%, photosynthetic bacteria 15%, pantoea agglomerans 34-42% by weight, mix, form the bacterial classification mixed liquor I, after insert the ferment-seeded jar by weight;
Four, the substratum of seeding tank is: brown sugar 6%, and dregs of beans 1-2%, potassium primary phosphate 0.2%, sodium-chlor 0.15%, light calcium carbonate 1%, peptone 0.5%, surplus is a water, and initial pH value is transferred to 7.0, temperature transfers to 121 ℃, sterilizes 30 minutes; Postcooling to 30 ℃, back press 4% access bacterial classification mixed liquor I of seeding tank material gross weight; Anaerobically fermenting 48-72 hour, rotating speed 180r/mi n, 32 ℃ of temperature when the pH value reaches 4.8, are fermentation termination when viable count reaches 1,500,000,000/ml, make liquid mixing bacterial classification NEM.
2. a kind of microbial organic fertilizer according to claim 1, it is characterized in that: described stalk, be made up of cornstalk, sorghum stalk, beans bar and straw, the weight percent of each material is: cornstalk 62-65%, sorghum stalk 10%, beans bar 10%, straw 15-18%.
3. a kind of microbial organic fertilizer according to claim 1 is characterized in that: described flour, to form by Semen Maydis powder and bean powder, and the weight ratio of each material is: Semen Maydis powder 50-70%, bean powder 30-50%.
4. the preparation method of a kind of microbial organic fertilizer according to claim 1, step is as follows:
Step 1, earlier straw powder is broken into the particle of 5-7mm, standby;
Step 2, get stalk 65-70%, flour 12-15%, lime 1%, potassium primary phosphate 0.5% by weight, mix and stirred 2 minutes, the back adds the water of 13.5-21.5%, mixes and stirs 3-5 minute;
Step 3, the compound that step 2 is made are sent in the pressurized vessel, send into steam in pressurized vessel, make the air pressure in the pressurized vessel rise to 1.5-2Mpa, and temperature rises to 160-180 ℃, sterilized 10 minutes, after material is sent into fermentation cabin;
After step 4, material were sent into fermentation cabin, by the 5-10% access liquid mixing bacterial classification NEM of fermentation materials gross weight in the fermentation cabin, anaerobism was cultivated 72-96 hour, 37 ℃ of culture temperature when the pH value is 4.5-5.0, are fermentation termination when viable count reaches 2,000,000,000/ml, deliver from godown, pack makes product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010558843.5A CN102079674B (en) | 2010-11-25 | 2010-11-25 | Microorganism organic fertilizer and preparation method thereof |
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Cited By (11)
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| CN102391964A (en) * | 2011-11-12 | 2012-03-28 | 江苏天象生物科技有限公司 | Bacillus medium |
| CN102488120A (en) * | 2011-12-28 | 2012-06-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN103524222A (en) * | 2013-10-12 | 2014-01-22 | 李春练 | Rice biological fertilizer and production method thereof |
| CN103773717A (en) * | 2013-12-30 | 2014-05-07 | 中北大学 | High-efficiency microbial fertilizer |
| CN104694422A (en) * | 2015-02-11 | 2015-06-10 | 齐齐哈尔大学 | Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons |
| CN104946550A (en) * | 2014-05-12 | 2015-09-30 | 山东黎昊源生物工程有限公司 | Leaf-protection biological strain repair agent |
| CN105950185A (en) * | 2016-07-12 | 2016-09-21 | 中国科学院地理科学与资源研究所 | Environment-friendly soil remediation agent as well as preparation method and application thereof |
| CN106045652A (en) * | 2016-01-27 | 2016-10-26 | 宜兴市天信生态种养专业合作社 | Organic compound-inoculant insecticide-fertilizer for preventing and treating lepidoptera pests on paddy rice and application thereof |
| CN106495820A (en) * | 2016-01-13 | 2017-03-15 | 中弘新缘生物科技（天津）有限公司 | A kind of preparation method of concentrated type composite microbiological fertilizer |
| CN107253890A (en) * | 2017-05-27 | 2017-10-17 | 镇江鑫源达园艺科技有限公司 | A kind of soil complex repairation agent |
| CN108996711A (en) * | 2018-09-11 | 2018-12-14 | 江苏远山生物技术有限公司 | A kind of compound micro-ecological preparation used for aquiculture and preparation method thereof containing clostridium butyricum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391964A (en) * | 2011-11-12 | 2012-03-28 | 江苏天象生物科技有限公司 | Bacillus medium |
| CN102391964B (en) * | 2011-11-12 | 2013-03-13 | 江苏天象生物科技有限公司 | Bacillus medium |
| CN102488120A (en) * | 2011-12-28 | 2012-06-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN102488120B (en) * | 2011-12-28 | 2013-02-13 | 广西东园生态农业科技有限公司 | Preparation method of fermenting ensilaged feed |
| CN103524222B (en) * | 2013-10-12 | 2016-01-20 | 广东兆晖生物科技有限公司 | A kind of Rice biological fertilizer and production method thereof |
| CN103524222A (en) * | 2013-10-12 | 2014-01-22 | 李春练 | Rice biological fertilizer and production method thereof |
| CN103773717A (en) * | 2013-12-30 | 2014-05-07 | 中北大学 | High-efficiency microbial fertilizer |
| CN104946550A (en) * | 2014-05-12 | 2015-09-30 | 山东黎昊源生物工程有限公司 | Leaf-protection biological strain repair agent |
| CN104694422A (en) * | 2015-02-11 | 2015-06-10 | 齐齐哈尔大学 | Separated pantoea ananatis HYL01 strain and application thereof in promotion of root growth of watermelons |
| CN106495820A (en) * | 2016-01-13 | 2017-03-15 | 中弘新缘生物科技（天津）有限公司 | A kind of preparation method of concentrated type composite microbiological fertilizer |
| CN106045652A (en) * | 2016-01-27 | 2016-10-26 | 宜兴市天信生态种养专业合作社 | Organic compound-inoculant insecticide-fertilizer for preventing and treating lepidoptera pests on paddy rice and application thereof |
| CN106045652B (en) * | 2016-01-27 | 2019-10-25 | 宜兴市天信生态种养专业合作社 | A kind of organic composite bacterium medicine fertilizer and its application for preventing and treating rice lepidoptera pest |
| CN105950185A (en) * | 2016-07-12 | 2016-09-21 | 中国科学院地理科学与资源研究所 | Environment-friendly soil remediation agent as well as preparation method and application thereof |
| CN105950185B (en) * | 2016-07-12 | 2019-08-02 | 中国科学院地理科学与资源研究所 | A kind of environment protection type soil renovation agent and preparation method thereof, application |
| CN107253890A (en) * | 2017-05-27 | 2017-10-17 | 镇江鑫源达园艺科技有限公司 | A kind of soil complex repairation agent |
| CN108996711A (en) * | 2018-09-11 | 2018-12-14 | 江苏远山生物技术有限公司 | A kind of compound micro-ecological preparation used for aquiculture and preparation method thereof containing clostridium butyricum |
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