CN106045652A - Organic compound-inoculant insecticide-fertilizer for preventing and treating lepidoptera pests on paddy rice and application thereof - Google Patents
Organic compound-inoculant insecticide-fertilizer for preventing and treating lepidoptera pests on paddy rice and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention discloses an organic compound-inoculant insecticide-fertilizer for preventing and treating lepidoptera pests on paddy rice. The organic compound-inoculant insecticide-fertilizer comprises organic fertilizer and six biological compound inoculants which are composed of Trichoderma viride, Bacillus licheniformis, Streptomyces spirolavendulae, Bacillus subtilis, Bacillus thuringiensis and Candida. The organic compound-inoculant insecticide-fertilizer provided by the invention can improve the disease resistance and stress resistance of rice plants, prevent and treat lepidoptera pests, and prevent and control rice blast and sheath blight of paddy rice, and has an action period of about 40 days.
Description
Technical field
The invention belongs to agricultural technology field, particularly to a kind of organic composite bacterium for preventing and treating Oryza sativa L. lepidoptera pest
Medicine is fertile and applies, and this compound bacteria medicine fertilizer can be used for preventing and treating the Lepidoptera evils such as rice leaf folder, striped rice borer, pink rice borer, rice plant skipper
Worm, simultaneously prevention and control rice blast, banded sclerotial blight.
Background technology
Agricultural ecological balance is broken already at present, and the epoch relying on natural enemy to control rice pest have not existed, water
Rice produces has to rely on spraying pesticide control rice pest, to reach to protect the purpose of product volume increase.Show according to relevant test,
In the case of the agricultural operation measures such as the continuous rice cultivation in same plot, does not use any pesticide, fertilising are carried out as usual, nearly 20 years
Having only 2 years of rice harvest, per mu yield only more than 50 kilogram, remaining time is all that No kernels or seeds are gathered, as in a year of scarcity.This absolutely proves agricultural ecological
System is the most fragile, and ecological balance has been broken, and uses the act that pesticide control rice pest is the certainty of Rice Production.
Chemical pesticide has efficient insect pest preventing and controlling effect, but often has great potential hazard to ecological environment, therefore, uses life
Thing composite bacterial fertilizer preventing and treating Oryza sativa L. lepidoptera pest, is to realize ensureing "rice bag" program and to safeguard that Agro-ecology balances strategic
Doulbe-sides' victory behave.
Oryza sativa L. is to produce in the environment of hot and humid, and during growth, multiple pest and disease damage frequently occurs, accurately and efficiently
Preventing and treating rice pest is to ensure that the key measure of Oryza sativa L. high yield good harvest.For many years, high in organic phosphates, carbamates etc.
Poison pesticide is extensively used control rice pest, and saving of labor saves this, and a medicine is multiplex, but also bring kill rice grub natural enemy,
Polluted-water, destroy the side effect such as agroecological environment, and the residual of agricultural product has a strong impact on the health of human body.At whole world model
In enclosing, limiting and use high-toxic pesticide to become common recognition, low-toxin farm chemicals, micro-poison pesticide, the production of biological pesticide and use are the most luxuriantly
Rise, become the target of governments at all levels and the eager desire of consumers in general.But biological pesticide is expensive, as Oryza sativa L. makes
Using middle high-toxic pesticide, pesticide cost is only the 8% of Oryza glutinosa total income, prevents and treats number of times about 4 times, and uses biological pesticide, pesticide
Cost is the 15% of Oryza glutinosa total income, and preventing and treating number of times 6-8 time, the benefit of rice cultivation will be decreased obviously, and this is vast farmers
Be unwilling accept.
The direction of biological pesticide preventing and treating rice pest development beyond doubt, governments at all levels strengthen subsidy dynamics, widely popularize
Biological pesticide.Rice sheath blight disease all prevented and treated by the biological pesticides such as jingganmycin, cnidiadin, wax bud bacterium, bacillus subtilis, right
The lepidoptera pests such as preventing and treating rice leaf folder, pink rice borer, striped rice borer, yellow rice borer, rice plant skipper, currently mainly have Bacillus thuringiensis
(BT), but preventive effect is poor, and effect duration is short, expensive, and vast farmers is reluctant to use, currently mainly organic paddy rice, organic vegetables
Use on dish.Japan, Vietnam and some place of China, by promoting duck-raising enclosed paddy fields, reach to control the purpose of rice pest,
But rice-duck farming is under conditions of hot and humid, duck is easy to morbidity, and the phenomenon of duck death is very universal, and have is the most complete
Army is annihilated, although at present by vaccinating and introducing degeneration-resistant new product duck, the problem of the large quantities of death of duck solves the most substantially, but peasant
The mental status disliked trouble generally exists, and the rise of the price of labour power forces rice-duck farming can only make in organic paddy rice cultivation
With, general rice field rice-duck farming technology is difficult to push open.
In recent years, the great pest and disease damage of this area Oryza sativa L. is the lepidoptera pests such as situation occurred frequently, particularly rice leaf roller, from
Within 2008 the most continuous 6 years, occur greatly, bring serious hidden danger to Oryza sativa L. safety in production, along with high poison persistent pesticide gradually
Eliminating, the difficulty of the preventing and treating great pest and disease damage such as rice leaf roller, snout moths larva, by increasing, is used for preventing and treating Oryza sativa L. lepidoptera pest rice
Leaf-roller, efficient, low toxicity, the less-persistent pesticide of snout moths larva, optional kind is few.Therefore, the preventing and treating of NEW TYPE OF COMPOSITE bacterium medicine fertilizer is implemented
Oryza sativa L. lepidoptera pest technical research and application project is to adapt to the reality need of current rice disease and pest preventing and treating, for saving labour force
And cost, fertile medicine integration has become current research direction, but fertile medicine integration is mostly fertilizer and chemical herbicide at present
Integration, fertilizer and the integrated of chemical insecticide and sustained-release pesticides, application effect poor, there is no substantial progress.
Summary of the invention
It is an object of the invention to overcome above-mentioned weak point, it is provided that a kind of by rice root absorption organic composite biological bacterium
Secretions kill lepidoptera pest, strengthen paddy disease-resistant, anti-adversity ability, the long period effectively control rice leaf folder,
The lepidoptera pests such as striped rice borer, pink rice borer, rice plant skipper, reduce insect pest preventing and controlling number of times, reduce the usage amount of chemical pesticide, meet green
The production requirement of food.
It is an object of the invention to be accomplished by:
A kind of organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest is fertile, including fertilizer and six kinds of composite bacteria agent capables,
Wherein: biological compound fungi agent is by Hypocrea virens, bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, Su Yun
Gold bacillus and candidiasis composition.
In described composite bacteria agent capable, the weight proportion of various biological bacteria is: 1-1.8:1-1.8:1-1.8:1-1.8:1-1.8:
1-1.8。
Hypocrea virens/gram Han bacterium number 0.1 hundred million that above-mentioned organic composite bacterium medicine fertilizer uses；Bacillus licheniformis number Han bacterium
0.1 hundred million/gram, spiral Strepiomyces lavendulae/gram Han bacterium number 0.1 hundred million, bacillus subtilis/gram Han bacterium number 0.1 hundred million, false silk
Yeast/gram Han bacterium number 0.1 hundred million, Bacillus thuringiensis/gram Han bacterium number 0.1 hundred million.
Above-mentioned fertilizer is fowl and animal excrement and straw decomposing fermented product.
Fertilizer can be prepared by following steps: fowl and animal excrement and stalk are added zymocyte and is sufficiently mixed
After, cover tightly with Polypropylence Sheet, through 24 hours, heap Nei Wenduda 45 DEG C-50 DEG C, within 48 hours, temperature reached 65 DEG C-70 DEG C, 10-15
My god, the raw material after fermentation all becomes thoroughly decomposed, overstrike or pitchy, then cools down, is dried to water content 21%, fertilizer.
The part by weight of fertilizer and biological compound fungi agent is 98.5-99.1:1.5-0.9.
The preparation method that the above-mentioned organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest is fertile comprises the following steps:
A), biological bacteria separates: by Hypocrea virens, Bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis
Bacterium, Bacillus thuringiensis, candidiasis respectively enter fermentation tank breeding, then breeding biological bacteria are air-dried respectively, make raw
Thing bacterium is in a dormant state；
B), by Hypocrea virens, Bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, Su Yun gold bar
Bacterium, candidiasis and fertilizer fully mix and stir, and make organic compound bacteria medicine fertile.
8, the preparation side that the organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest according to claim 7 is fertile
Method, it is characterised in that after fowl and animal excrement and stalk are sufficiently mixed, cover tightly with Polypropylence Sheet, through 24 hours, temperature in heap
Reaching 45 DEG C-50 DEG C, within 48 hours, temperature reaches 65 DEG C-70 DEG C, and 10-15 days, the raw material after fermentation all became thoroughly decomposed, overstrike or black
Brown, then cool drying is to water content 21%, fertilizer.
Organic composite bacterium medicine of the present invention is fertile to be applied in preventing and treating Oryza sativa L. lepidoptera pest, and wherein, Oryza sativa L. lepidoptera pest is excellent
Select rice leaf roller, striped rice borer, pink rice borer, rice plant skipper.
Organic composite bacterium medicine of the present invention is fertile to be applied in prevention and preventing and treating rice fungus diseases evil, wherein, and rice fungus diseases evil
Preferably rice blast, banded sclerotial blight.
The occupation mode that organic composite bacterium medicine of the present invention is fertile is: incubate Sheng phase use 1 time at Oryza sativa L. lepidoptera pest ovum, every time
Mu executes 8-12 kilogram, in the case of keeping field water layer, is valid up to more than 40 day, use continuously 2 times (at the beginning of the first tenday period of a month in July and
Early August Mo), it is better than the preventive effect of routine administration 5-6 time, and has one fertile multiplex, environmentally friendly, with person poultry safety, saving of labor
Laborsaving pollution is few, small investment and output capacity is high, there is certain using value and DEVELOPMENT PROSPECT.
Strain group and Main Function mechanism that the present invention has screened is: Hypocrea virens, bacillus licheniformis, mainly makees
With being induction plant antibody protein, decomposing organic breeding for other strain at rice terrace provides nutrition；Streptomyces griseus spiral
Mutation, bacillus subtilis are preventing and treating rice fungus diseases evils；Bacillus thuringiensis, false screw bacterium, with the common effect of other strain
Under, being combined with fertilizer, under the ecological environmental condition of rice terrace, activate breeding, its secretions can effectively prevent and treat water cnaphalocrocis
The lepidoptera pests such as snout moth's larva, striped rice borer, pink rice borer, rice plant skipper, the most effectively prevention and control rice blast, banded sclerotial blight.Six kinds of biological floras
The rational proportion fallen, strain acts on down jointly kills Oryza sativa L. lepidoptera pest larva and ovum, the most effective prevention and control rice blast,
Banded sclerotial blight.
Microbial inoculum is sufficiently mixed by the present invention with organic matter, containing biological bacteria 0.5-0.6 hundred million/gram, and is in sufficient dormancy
In state, and keep being dried of bacterial manure, the content of organic matter 25%, provide nutrition and energy for strain in rice terrace breeding, promote
The Fast-propagation of strain and regeneration, reach to improve the purpose of preventive effect, guarantees that the drug effect phase was up to about 40 days simultaneously.
The present invention collects agricultural biological bacterium and more than 20 population of medical bio bacterium in continuous 5 years in 2010-2014, carries out many
Plant the field test of configuration biological bacteria combination, more than 100, annual experimental plot, Preliminary screening Hypocrea virens, lichens spore bar
Bacterium, spiral Strepiomyces lavendulae, bacillus subtilis, Bacillus thuringiensis, six probiotics groups of candidiasis, joined by difference
Ratio, is developed into compound bacteria medicine fertilizer with fertilizer, activates breeding under the ecological environment of rice terrace, and its secretions can effectively kill water
The larva of the lepidoptera pests such as rice leaf roller, striped rice borer, pink rice borer, rice plant skipper and ovum, the most effectively prevention and control rice blast, stricture of vagina
Rot, in the case of keeping field water layer, is valid up to more than 40 day, uses 2 times, and the preventive effect of bio-bacterial manure is better than conventional use
The preventive effect that medicine is 5-6 time.The demonstration of 2012-2014 field experiment and large-area applications effect are notable, have one fertile multiplex, to environment
Close friend, to person poultry safety, pollutes few, and small investment and output capacity are high, have certain practical value and DEVELOPMENT PROSPECT.
Compared with prior art, the biological characteristics that the present invention occurs according to rice disease and pest, utilize biological composite bacterial manure master
Rice plant is disease-resistant, anti-adversity ability to strengthening to want group, prevents and treats Oryza sativa L. lepidoptera pest, effectively kills its larva and ovum, be to be
System learns thought establishment ecological regulation and control technology, creates one and is conducive to utilizing BIOLOGICAL CONTROL, and is unfavorable for that harmful organism is developed
Environmental condition.
The present invention is also equipped with following characteristic:
1, the present invention is according to Oryza sativa L. harmful organism biological inoculum system, whole process theories of ecosystem and technology, root
Carry out gain according to economic threshold values day product module type and press down evil, by regulation ecological factors and the integrated association to Oryza sativa L. management technique measure
Adjusting, the effect resonance making biological composite bacterial manure control Oryza sativa L. lepidoptera pest is amplified, thus decreases the number of times of insect pest preventing and controlling, subtracts
The light cost of rice disease and pest preventing and treating, protects farmland ecological environment, also makes peasant be eased from heavy physical work,
Be conducive to soil to farming expert collect neutralize agricultural modernization process, application organic-biological composite bacterial fertilizer strengthen paddy disease-resistant,
Anti-adversity ability, and there is fixed nitrogen, phosphorus decomposing, the effect of potassium decomposing, the activity of favourable soil microorganism, soil improvement is played good
Effect, promotes complex ecological agricultural system, efficiently solves the drawback of tradition Rice Cropping industry " low utilization, maximum discharge ".
2, the present invention has the innovation of pollution-free food production technology, utilizes the advance of its organic composite bacterium, practicality, changes
Becoming the mode of production of original Green Rice, produce for Green Rice industrialization, standardization is processed, and branding is sold and carried
For reliable technical support, thus improve constantly the economic benefit of rice cultivation.The present invention is new by developing rice disease and pest preventing and treating
Pattern, the technology producing green rice for innovation is put into effect.
3, the present invention has the innovation of fertile medicine integration.For saving labour force, current China means of agricultural production production industry starts
The upsurge of one fertile medicine integration, the medicine fertilizer kind at U.S.'s preventing and treating diseases and pests of agronomic crop has more than 200, and China's only preventing and treating
A kind of medicine of subterranean pest-insect is fertile.It is fertile that the present invention screens development of new organic composite biological bacterium medicine, is entirely the innovation of a kind of science and technology.
, ear manuer fertile in conjunction with rice tillering uses, and both can strengthen disease-resistant, anti-adversity ability, and also can prevent and treat Oryza sativa L. lepidoptera pest, Sheng Gongjie
This, one fertile (medicine) is multiplex.
4, the present invention has the innovation of pest control techniques pattern, and the well ridge the most either preventing and treating rice sheath blight disease is mould
Element, or the pesticide Bacillus thuringiensis (BT) etc. of preventing and treating Oryza sativa L. lepidoptera pest, be all to be watered to be sprayed on plant and blade, ooze
Thoroughly arrive in plant body, reach to prevent and treat the purpose of rice disease and pest.And the fertile application of novel organic composite biological bacterium medicine changes tradition
Rice disease and pest control models, the microorganism of dormancy is activated behind rice field by applying, a bacterium is equivalent to a factory, at water
Breeding rapidly in rice field and discharge substantial amounts of secretions, in conjunction with rice tillering, fertile or ear manuer, uses organic composite bacterium medicine fertilizer 1-2
Secondary, each mu executes 8-12 kilogram, relies on rice root to absorb its secretions, kills Oryza sativa L. lepidoptera pest, at rice growing season
Between only need to prevent and treat the disease pest such as 2-3 planthopper, banded sclerotial blight, rice blast, thus reduce and prevent and treat number of times and reach about 50%, chemical pesticide
Reducing 50-60%, mu pesticide cost declines 50-70 unit.
5, the utilization of biological bacteria, one is as Fertilizer application, has environmentally friendly, and beneficially the improvement of soil, overcomes
The benefits such as the obstacle that plant growth is caused by continuous cropping；Two is that biological bacteria development belongs to biological pesticide, sprays crop surface and can prevent
Curing the disease insect pest, as crop pest prevented and treated by the biological pesticides such as jingganmycin, wax bud bacterium, bacillus subtilis, Bacillus thuringiensis is biological
Pesticide control crops lepidoptera pest；Three is fertile medicine integration, and the bio-fertilizer of development is absorbed by crop root, both as fertile
Material uses, and can prevent and treat again diseases and pests of agronomic crop.
Detailed description of the invention
Below in conjunction with specific embodiment, screened by probiotics and compound bacteria medicine fertilizer is developed and field control effectiveness test demonstration comes
Further appreciate that the present invention, develop in conjunction with probiotics screening in 3 years and compound bacteria medicine fertilizer and imitate experiment and demonstration, example with field medicine (fertile)
Property explanation and help to be further appreciated by essence of the present invention, but probiotics screening and compound bacteria medicine fertilizer are developed and are tried with field medicine (fertile) effect
Test demonstration and large-area applications illustrates that the present invention, do not represent whole technical schemes under present inventive concept, the most should not
Understanding the restriction to technical scheme, the technical characteristic in other words with same or analogous technique effect is simple
Change or replace and all belong to protection scope of the present invention.
Embodiment 1
The preparation that organic composite bacterium medicine is fertile:
1, biological bacteria separates: by Hypocrea virens, bacillus subtilis, Bacillus thuringiensis, candidiasis, lichens spore
Bacillus, spiral Strepiomyces lavendulae respectively enter fermentation tank breeding, then breeding biological bacteria are air-dried respectively, make biological bacteria
In a dormant state.
2, organic gather: the organic matters such as fowl and animal excrement are added after zymocyte is sufficiently mixed, cover tightly with Polypropylence Sheet, through 24
Hour heap Nei Wenduda about 50 DEG C, within 48 hours, temperature can rise to about 70 DEG C, and 10-15 days, the raw material after fermentation was the most rotten
Ripe, worm's ovum, grass seed and source of disease bacterium in raw material are killed entirely, raw material overstrike or pitchy, the organic cooling after fermentation
Be dried to water content 21%, fermentation material standby.
3, by Hypocrea virens, Bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, Bacillus thuringiensis,
Candidiasis, fertilizer are fully mixed and stirred by the part by weight of 1:1:1:1:1:1:594, are developed into particle medicinal fertilizer.Then seal,
Packaging gets product.In this medicine fertilizer: containing biological bacteria 0.6 hundred million/gram (wherein, Hypocrea virens/gram Han bacterium number 0.1 hundred million, lichens
Bacillus cereus/gram Han bacterium number 0.1 hundred million, spiral Strepiomyces lavendulae, containing bacterium number 0.1 hundred million/gram, bacillus subtilis number Han bacterium
0.1 hundred million/gram, candidiasis/gram Han bacterium number 0.1 hundred million, Bacillus thuringiensis/gram Han bacterium number 0.1 hundred million).
Wherein, Hypocrea virens, Bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, candida mycoderma
Bacterium, Bacillus thuringiensis are outsourcing microbial inoculum, have new company, Shandong green moral ground biological from Yancheng of Jiangsu Province god biotechnology slightly respectively
Science and technology has new company, grossness Di Fei industry company limited of Nanning City to buy, and fertilizer is local fowl and animal excrement and straw decomposing fermentation
Thing.
Hypocrea virens, bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, Bacillus thuringiensis, false silk
Yeast will produce secretion antibiotic after application in a large number, and rice disease and insect pest are played prevention and control effect.
The organic composite bacterium medicine fertilizer that example 2 below-4 is used all prepares according to embodiment 1 method.
Embodiment 2 field of the present invention medicine (fertile) effect wide field trial (2012)
Experimental condition and method: test site tests (Peng Zhuan village, Xin Jie street) base in Jiangsu Province's Yixing City regionality disease pest
Ground is implemented, and soil property is yellow soil, pH value 6.6, and front stubble is Semen Tritici aestivi, and planting type is water direct seeding, June 5 sowing date, and kind is military
Fortune round-grained rice 23.If 4 process: 1. respectively execute fertile 10 kgs/acre of organic composite bacterium medicine on July 5 and August 10；2. executed July 5
Fertile 10 kgs/acre of machine compound bacteria medicine；3. fertile 10 kgs/acre of organic composite bacterium medicine was executed on August 3；4. conventional pesticide compares for 6 times,
Great Qu area 333.3m2.Separately setting and do not execute organic-biological bacterium medicine fertilizer with insecticide as check plot (CK), plot area is 33.3m2,
Irregularly observe the control worm effect to lepidoptera pest, period of maturation examination tree characteristics after executing organic composite bacterium medicine fertilizer, calculate and increase
Producing effect, result of the test refers to table 1.
Preventive effect computing formula:
Conclusion: control worm effect is notable.Result it can be seen that organic composite biological bacterium medicine fertile to phosphorus wing order rice leaf roller,
Pink rice borer, rice plant skipper have specially good effect, and the fertile metabolite produced in the reproductive process of field of this biological bacteria medicine, to lepidopterous larvae, ovum
Having direct killing effect and superinfection insecticidal action, so medicine can last till next from generation to generation, investigation display is once (July 5)
Executing fertile 10 kilograms of organic composite bacterium medicine, within 30 days, control worm effect reaches 97.6-100%, and final (70 days) control worm effect reaches 77.4-
82.6%；Once (August 1) execute fertile 10 kilograms of organic composite bacterium medicine, final (45 days) control worm effect reaches 95.4-97.9%.Two
Secondary (July 5 and August 10), mu respectively executed fertile 10 kilograms of organic composite bacterium medicine, and control worm effect maintains 97.1-100% always.
Obvious effect of increasing production.Execute organic composite bacterium medicine fertilizer once (10 kgs/acre) respectively on July 5 and August 1 with conventional
Pesticide is compared for 6 times, increases production 7.5%, 10.6% respectively.Use the volume increase 14.3% of secondary, with conventional fertilizer application not medication (CK) phase
Ratio, uses once (July 5, August 1) and increases production 15.0%, 18.3% respectively, uses the increasing in secondary (July 5 and August 1)
Produce 22.3%.
Embodiment 3 field of the present invention medicine (fertile) effect wide field trial demonstration (2013)
Test method: experiment and demonstration place is implemented in Chen Qiao village, Yixing City Zhou Tie town, Jiangsu Province, and soil property is hargil, PH6.4,
The content of organic matter is medium, and front stubble is Semen Tritici aestivi, and planting type is water direct seeding, rice varieties force fortune round-grained rice 23, and sowing date is June 2,
The row's of filling facility and water conservation condition are preferable, carry out different time with embodiment organic composite bacterium medicine fertilizer and once execute that (medicine) is fertile and secondary is executed
Medicine fertilizer experiment and demonstration, sets 4 process: 1. 10 public affairs respectively used by organic composite bacterium medicine fertile (July 12) and (August 9) as requested
Pound/acre；2. organic composite bacterium medicine fertile (July 12) uses 10 kgs/acre；3. 10 public affairs used by organic composite bacterium medicine fertile (August 9)
Pound/acre；4. conventional 6 medications compare, and great Qu demonstrates area 667m2；Separately set and do not execute organic composite bacterium medicine fertilizer and insecticide work
Check plot (CK), plot area 33m2, irregularly observe the control worm effect to lepidoptera pest after using organic composite bacterium medicine fertilizer,
Last period of maturation examination tree characteristics, calculates effect of increasing production.Great Qu demonstration result refers to table 2.
Preventive effect computing formula:
Preventive effect (%)=do not execute worm amount (CK)-treatment region worm amount × 100% that (medicine) is fertile
Do not execute the worm amount (CK) that (medicine) is fertile
Conclusion: control worm effect is notable.From 2013 years organic composite bacterium medicine fertilizer preventing and treating Oryza sativa L. lepidoptera pest experiment and demonstration,
Result can it is further seen that, organic composite bacterium medicine fertilizer Lepidoptera rice leaf roller, pink rice borer, rice plant skipper are had specially good effect, by executing
Enter and the microorganism of dormancy activated behind rice field, breed rapidly in rice terrace and discharge substantial amounts of secretions, to lepidopterous larvae,
Ovum has direct killing effect and superinfection insecticidal action, so drug effect can last till next from generation to generation, and investigation display further,
Executing fertile 10 kilograms of organic composite bacterium medicine on July 12, within 20 days, control worm effect reaches 98.0%-100%, final (63 days) control worm effect
80.3-90.1%.Executing composite organic bacterial fertilizer 10 kilograms August 9, final (36 days) control worm effect reaches 95.6-98.5%；7
Respectively executing fertile 10 kilograms of organic composite bacterium medicine on the moon 12 and August 9, control worm effect maintains 98.2-100% always.
Effect of increasing production is obvious: execute respectively on July 12 and August 91 organic composite bacterium medicine fertile 10 kgs/acre with conventional 6 times
Medication is compared, and increases production 3.15%, 3.45% respectively, uses the volume increase 7.8% of 2 times.With conventional dispenser, without Lepidoptera medicine (CK)
Compare, increase production 8.3%, 9.0% respectively, use the volume increase 13.2% of 2 times.
Embodiment 4 field of the present invention medicine fertilizer efficiency large-area applications (2014)
Test method: Demonstration Application place: Demonstration Application base, Yixing City Fang Qiao town, Jiangsu Province is implemented, and soil property is hargil,
PH6.5, the content of organic matter is medium, and front stubble is Semen Tritici aestivi, and planting type is transplanting, the machine transplanting of rice on June 12, kind south round-grained rice 46.If 3
Process (including CK): 1. fertile 10 kgs/acre of organic composite bacterium medicine；2. conventional 5 medications compare；The most do not execute organic composite bacterium medicine
Fertile and insecticide compares (CK), Demonstration Application area 1100 mu, irregularly observes Lepidoptera after executing organic composite bacterium medicine fertilizer
Insect control worm effect, in using latter 20 days, 40 days investigation control worm effects, examine or check tree characteristics before receiving rice, calculate effect of increasing production.Show
Model is applied the results detailed in Table 3.
Preventive effect computing formula
Conclusion: from large-area applications as a result, it was confirmed that Lepidoptera rice leaf roller, big can effectively be killed by organic composite bacterium medicine fertilizer
Snout moth's larva, rice plant skipper larva and ovum, investigation display, composite organic bacterial fertilizer 10 kilograms was executed August 3, within 20 days, control worm effect reaches 96.5-
99.5%, final (40 days) control worm effect is all more than 95%.Thus decrease insect pest preventing and controlling number of times, alleviate insect pest preventing and controlling
Cost, protects farmland ecological environment.Enhance paddy disease-resistant, anti-adversity ability simultaneously, and there is fixed nitrogen, phosphorus decomposing, the work of potassium decomposing
With, be conducive to volume increase.Period of maturation examines or check, and uses fertile 10 kgs/acre of organic composite bacterium medicine for 1 time August 3, with conventional 5 medications
Compare, increase production 9.54%, volume increase 17.5% compared with CK.
Claims (10)
1. one kind fertile for preventing and treating the organic composite bacterium medicine of Oryza sativa L. lepidoptera pest, it is characterised in that include fertilizer and six kinds
Biological compound fungi agent, wherein: biological compound fungi agent is talked purple ash streptomycete, hay by Hypocrea virens, bacillus licheniformis, spiral
Bacillus cereus, Bacillus thuringiensis and candidiasis composition.
Organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest the most according to claim 1 is fertile, it is characterised in that institute
In the composite bacteria agent capable stated, the weight proportion of various biological bacteria is: 1-1.8:1-1.8:1-1.8:1-1.8:1-1.8:1-1.8.
Organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest the most according to claim 1 and 2 is fertile, it is characterised in that
Described Hypocrea virens/gram Han bacterium number 0.1 hundred million, Bacillus licheniformis/gram Han bacterium number 0.1 hundred million, spiral Strepiomyces lavendulae,
Containing bacterium number 0.1 hundred million/gram, bacillus subtilis/gram Han bacterium number 0.1 hundred million, candidiasis/gram Han bacterium number 0.1 hundred million, Su Yun
Gold bacillus/gram Han bacterium number 0.1 hundred million.
Organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest the most according to claim 1 is fertile, it is characterised in that institute
The ammonium humate stated is fowl and animal excrement and straw decomposing fermented product.
Organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest the most according to claim 4 is fertile, it is characterised in that have
Machine is fertile through the following steps that prepare: after fowl and animal excrement and stalk being sufficiently mixed, covers tightly with Polypropylence Sheet, passes through
24 hours, heap Nei Wenduda 45 DEG C-50 DEG C, within 48 hours, temperature reached 65 DEG C-70 DEG C, and 10-15 days, the raw material after fermentation was the most rotten
Ripe, overstrike or pitchy, then cool down, be dried to water content 21%, fertilizer.
Organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest the most according to claim 1 is fertile, it is characterised in that have
Fertile and composite bacteria agent capable the part by weight of machine is 98.5-99.1:0.9-1.5.
7. the preparation method of the organic composite bacterium medicine fertilizer for preventing and treating Oryza sativa L. lepidoptera pest described in claim 1, its feature
It is that the method comprises the following steps:
A), biological bacteria separates: by Hypocrea virens, Bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, Soviet Union
Cloud gold bacillus, candidiasis enter fermentation tank breeding, then breeding biological bacteria are air-dried respectively, make biological bacteria be in not
Dormancy state；
B), by Hypocrea virens, Bacillus licheniformis, spiral Strepiomyces lavendulae, bacillus subtilis, Bacillus thuringiensis, vacation
Silk yeast and fertilizer, by fully mixing and stirring, make organic compound bacteria medicine fertile.
The preparation method that organic composite bacterium medicine for preventing and treating Oryza sativa L. lepidoptera pest the most according to claim 7 is fertile, its
After being characterised by being sufficiently mixed fowl and animal excrement and stalk, cover tightly with Polypropylence Sheet, through 24 hours, heap Nei Wenduda 45
DEG C-50 DEG C, within 48 hours, temperature reaches 65 DEG C-70 DEG C, and 10-15 days, the raw material after fermentation all became thoroughly decomposed, overstrike or dark brown
Color, then cool drying is to water content 21%, fertilizer.
9. described in claim 1-6 any one claim organic composite bacterium medicine fertile at preventing and treating Oryza sativa L. squama wing: mesh insect
In application, wherein, the preferred rice leaf roller of Oryza sativa L. lepidoptera pest, striped rice borer, pink rice borer, rice plant skipper.
10. the organic composite bacterium medicine fertilizer described in claim 1-6 any one claim is at prevention and preventing and treating rice fungus diseases
Application in evil, wherein, rice fungus diseases evil preferred rice blast, banded sclerotial blight.
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| CN108477222A (en) * | 2018-03-19 | 2018-09-04 | 叶虹 | A kind of biocontrol agent and its preparation process for preventing lepidopterous insect pest |
| CN108496659A (en) * | 2018-03-19 | 2018-09-07 | 叶虹 | The technique that lepidopterous insect pest is prevented using composite bacteria agent |
| CN110724534A (en) * | 2019-10-11 | 2020-01-24 | 云南探花农业科技开发有限公司 | Powdery biological soil conditioner |
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| CN102775202A (en) * | 2012-08-24 | 2012-11-14 | 江西龙驰生物工程有限公司 | Novel microbial organic fertilizer with anti-actinomyces active compound bacteria and preparation method thereof |
| CN103483020A (en) * | 2013-09-13 | 2014-01-01 | 成都天星农业科技有限公司 | Bio-organic fertilizer and method for preparing bio-organic fertilizer which prevents and treats soil insects |
| CN105061078A (en) * | 2015-08-18 | 2015-11-18 | 崔明华 | Health bio-organic fertilizer and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102079674A (en) * | 2010-11-25 | 2011-06-01 | 张培举 | Microorganism organic fertilizer and preparation method thereof |
| CN102775202A (en) * | 2012-08-24 | 2012-11-14 | 江西龙驰生物工程有限公司 | Novel microbial organic fertilizer with anti-actinomyces active compound bacteria and preparation method thereof |
| CN103483020A (en) * | 2013-09-13 | 2014-01-01 | 成都天星农业科技有限公司 | Bio-organic fertilizer and method for preparing bio-organic fertilizer which prevents and treats soil insects |
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Cited By (3)
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| CN108477222A (en) * | 2018-03-19 | 2018-09-04 | 叶虹 | A kind of biocontrol agent and its preparation process for preventing lepidopterous insect pest |
| CN108496659A (en) * | 2018-03-19 | 2018-09-07 | 叶虹 | The technique that lepidopterous insect pest is prevented using composite bacteria agent |
| CN110724534A (en) * | 2019-10-11 | 2020-01-24 | 云南探花农业科技开发有限公司 | Powdery biological soil conditioner |
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