CN105494448A - Preparation method of biological preparation for preparing and curing vegetable nematode disease - Google Patents

Preparation method of biological preparation for preparing and curing vegetable nematode disease Download PDF

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Publication number
CN105494448A
CN105494448A CN201610022029.9A CN201610022029A CN105494448A CN 105494448 A CN105494448 A CN 105494448A CN 201610022029 A CN201610022029 A CN 201610022029A CN 105494448 A CN105494448 A CN 105494448A
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CN
China
Prior art keywords
chitin
vegetables
nematodosis
biologic
paecilomyces lilacinus
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Pending
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CN201610022029.9A
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Chinese (zh)
Inventor
朱述尧
高贵云
裴德修
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SHANDONG YIFENGYUAN BIOTECHNOLOGY CO Ltd
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SHANDONG YIFENGYUAN BIOTECHNOLOGY CO Ltd
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Priority to CN201610022029.9A priority Critical patent/CN105494448A/en
Publication of CN105494448A publication Critical patent/CN105494448A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

Abstract

The invention discloses a preparation method of a biological preparation for preparing and curing a vegetable nematode disease. The preparation method is characterized in that cultivation is conducted on paecilomyces lilacinus through a culture medium firstly, after the paecilomyces lilacinus is cultivated to be mature, chitin is added to conduct mixed cultivation, and the biological preparation for preparing and curing the vegetable nematode disease is prepared. By means of the preparation method of the biological preparation for preparing and curing the vegetable nematode disease, the paecilomyces lilacinus and the chitin are well combined together and do not need to be used separately, use is convenient, the cost is low, and the effect is good. The prepared biological preparation for preparing and curing the vegetable nematode disease has the advantages that the soil fertility is improved, the yield is increased, a harvest is achieved, the quality is improved, the labor is saved, stress resistance and disease resistance of crops can be strengthened, the amount of pesticide is reduced, pesticide residues of agricultural products are degraded, the soil quality is improved, nematodes are eliminated, harmful bacteria are resisted, worm eggs are destroyed, and the chance of pests to continue the ancestral line is eradicated.

Description

A kind of preparation method preventing and treating the biologic product of vegetables nematodosis
Technical field:
The present invention relates to a kind of preparation method of biologic product, particularly relate to a kind of preparation method preventing and treating the biologic product of vegetables nematodosis.
Background technology:
The main harm part of root knot nematode disease to vegetables is vegetables root, wherein the side root of vegetables and fibrous root the most vulnerable.Root of being injured produces the knob or root knot that differ in size, and root knot is just white, soft, after become fawn or dark brown, rough surface, sometimes split.Cutting root knot open, can see white pearl granule, is the female adult pest of root-knot nematode.Vegetables grow after infecting by root-knot nematode slowly, and blade is little and yellow, and when morbidity is heavy, plant is short and small, shaky or abnormal seeding, and when meeting high temperature and drought weather, plant is easily wilted even withered.Nemas often has generation, harm seriously in China local, almost often kind of crop has 1-2 kind nematodosis, and occurring area expands sometimes, endangers more and more heavier, become one of problem demanding prompt solution in agricultural production.
Biological control prevents and treats the prevention and controls most in root knot nematode disease with application prospect at present, meet environmental protection and health idea, but the domestic biological prevention and control agent for preventing and treating root knot nematode disease is considerably less, great majority are all also in development to the effective biological prevention and control agent of root-knot nematode, and field result of use less stable.The biological prevention and control agent of current use only have nematode must gram and Avermectin.And more in root knot nematode control is Avermectin, available Abamectin carries out ditch spread or spreads manuer in holes, but there is nearly all biopesticide all inevitably weakness as biological pesticide avermectin, namely root knot nematode disease control is not thorough, and field result of use less stable.
Summary of the invention:
The object of this invention is to provide the preparation method of the biologic product of the control vegetables nematodosis that the control of a kind of root knot nematode disease is thorough, field result of use is stable.
To achieve these goals, technical scheme of the present invention is: a kind of preparation method preventing and treating the biologic product of vegetables nematodosis, it is characterized in that: first by the cultivation of Paecilomyces lilacinus by medium, after cultivating maturation, add the biologic product obtaining control vegetables nematodosis after chitin carries out Mixed culture again.
The cultural method of described medium is: get raw material beef extract 5-6 part by weight, peptone 3-4 part, sugared 100-200 part, phosphorous acid 10-20 part, potassium 10-20 part; Above-mentioned raw materials is put into fermentation tank and add water to 1000 parts, pH value is adjusted to 6.5-7.5, be warmed to 110-130 DEG C and keep 20-28 hour, cool the temperature to 30-34 DEG C afterwards.
Described medium, comprises the raw material of following weight proportion: raw material beef extract 5-6 part, peptone 3-4 part, sugared 100-200 part, phosphorous acid 10-20 part, potassium 10-20 part.
Described Paecilomyces lilacinus drops into fermentation tank, and stir oxygenation and cultivate, the cultivation temperature that described Paecilomyces lilacinus is cultivated is 28-32 DEG C, pH value is 6.5-7.5, cultivate and cultivate ripe culture fluid in 5-7 days, afterwards by chitin and culture fluid according to weight ratio 4-6: 6-4 ratio mix, the mixed liquor making Paecilomyces lilacinus and chitin continues cultivation 10 days more than and namely obtains this preparation.
Described chitin adds culture fluid in the mode of chitin solution.Chitin solution be prepared as known technology, no longer describe.Chitin mixes with the ratio of culture fluid according to weight ratio 5: 5.
In normal agro-farming, microorganism such as mold fungi etc. enters in soil, the effect of good physical soil melioration can be obtained, because the breeding of microorganism, except a large amount of polysaccharose mucus can be secreted, impel soil granular outside the pale of civilization, and various gas can be discharged, comprise carbonic acid gas, methane gas, average containing 100,000,000 microbial bodies among every gram of soil, the gas of its discharge and air pressure are enough to loose soil property, promote soil porosity and the ploughing and weeding degree of depth, hyphomycetic amount reproduction in microorganism, the procreation of its mycelia contributes to soil melioration, edaphon directly affects except the effect of soil physical properties except this type of, it also can secrete a large amount of biochemical substances, comprise amino acid, nucleic acid, carbohydrate, active ferment, and for improving the environment of oneself existence, microbes secretes some to the inhibited material of other microbe survival or adopt certain methods, as swallowed, parasitic or secretion Fungicidal substance and lysozyme etc., this is antagonism, and namely the microorganism that can produce antagonism is referred to as antagonistic microbe.Edaphon, if containing enough antagonistic microbe, then the pathogen in soil even in plant corpus can reduce, and becomes extinct even, so can reduce the demand of agricultural chemicals.And effective bacterial parasite of Paecilomyces lilacinus root-knot nematode and white Cyst nematode ovum just, to the percentage of egg parasitism of root-knot nematode up to 60%-70%, usefulness is prevented and treated to multiple nematode, its host has root-knot nematode, Cyst nematode, golden nematode, golden nematode, is the most promising biological prevention and control agent of control root-knot nematode.The inhibiting mechanism of Paecilomyces lilacinus to root-knot nematode is after Paecilomyces lilacinus contacts with nematode egg capsule, in adhesive matrix, the mycelia of biocontrol microorganisms Paecilomyces lilacinus surrounds whole worm's ovum, mycelia end is thicker, because the activity of metabolite and mycosin enzyme makes chorion top layer break, Paecilomyces lilacinus microbial inoculum is pure microorganism spore preparation alive, there is the features such as efficient, spectrum, long-acting, safe, pollution-free, noresidue, can obvious stimulation plant growth.Around root system of plant, use Paecilomyces lilacinus microbial inoculum obviously can not only suppress nematode infection, and the growth of root system of plant and plant nutrition organ can be promoted, as seed dressing, spread manuer in holes during field planting, to the sprouting of seed and growth of seedling, there is facilitation, can realize that seedling is complete, Miao Lv, Miao Zhuan, generally can make crop yield more than 15%.In the incubation of Paecilomyces varioti, particularly in medium and Submerged fermentation process, this bacterium can produce abundant derivative, the similar heteroauxin product of the first, the growth of root system of plant and plant is promoted when its most significant physiological function is low concentration, therefore execute this bacterium at root system of plant and obviously can not only suppress nematode infection, and the growth of plant nutrition organ can be promoted, also there is facilitation to the Germination and growth of seed simultaneously, this bacterium can produce abundant chitinase, chitinase has degradation to chitin, it can promote the hatching of line eggs, improve paecilomycerol to the parasitic rate of nematode, in addition lysis enzyme can also be produced, dextranase and silk-fibroin enzyme, Paecilomyces lilacinus can also promote the dissolving of difficultly-soluble phosphates, the solubilizing effect of paecilomycerol can reach about 30%.
The derivative that chitin is chitin hexose four de-acetyl and generates, chitin forms chitin by the effect of de-acetyl, its composition is closely related with the epigenetic structure of microorganism, can stimulate when chitin appears at animal and plant cells epidermis and give the information of cell mistake, as being have alien enemy (pathogen) close to the immune system of Cuticle of cell thus active cell, the formation of active cell antibody, what enhance metabolism is vigorous simultaneously, make cell acute activation tackle the state of all microorganisms intrusion, accelerate metabolism.Activating immune system and metabolic vigorous, its result is exactly cause growth vigorous concerning animals and plants, immunity resistance against diseases improves, simultaneously activating immune system and metabolic vigorous, fungi can invade and facilitates worm's ovum and replace, also can excrete poison and play toxic action to nematode, the relation of Here it is chitin and microorganism is the strongest with chitin hexa polyose.Chitin has protonated ammonium, protonated ammonium and the electronegative cell membrane effect of bacterium, absorption and coagulation bacterium, and penetration cell wall enters in cell simultaneously, the metabolism of upset bacterium and synthesis and have antibacterial action.
Compared with prior art, tool of the present invention has the following advantages: the preparation method of the biologic product of control vegetables nematodosis of the present invention, Paecilomyces lilacinus and chitin are well combined, need not separately use, easy to use, cost is low, effective.By the biologic product of the control vegetables nematodosis obtained by this preparation method, there is enhancing fertility, volume increase good harvest, oil recovery enhancement, save artificial, can strengthen that crop is degeneration-resistant, resistance against diseases, reduce Pesticide use amount, degraded agricultural product medicine is residual, improve soil property, eliminate nematode, harmful bacterium of contending with, destroy worm's ovum, stop the chance that insect is produced a male heir to continue the family line.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1
Prevent and treat a preparation method for the biologic product of vegetables nematodosis, for first Paecilomyces lilacinus being passed through medium culture, adding again after cultivating maturation after chitin carries out Mixed culture and obtaining.
Wherein, first obtained described medium is: get raw material beef extract 5-6 part by weight, peptone 3-4 part, sugared 100-200 part, phosphorous acid 10-20 part, potassium 10-20 part; Above-mentioned raw materials is put into fermentation tank and add water to 1000 parts, pH value is adjusted to 6.5-7.5, is warmed to 110-130 DEG C and keeps 20-28 hour, cool the temperature to 30-34 DEG C afterwards.Afterwards described Paecilomyces lilacinus is dropped into fermentation tank, and stir oxygenation and cultivate, the cultivation temperature that described Paecilomyces lilacinus is cultivated is 28-32 DEG C, pH value is 6.5-7.5, cultivate and cultivate ripe culture fluid in 5-7 days, afterwards by chitin and culture fluid according to weight ratio 4-6: 6-4 ratio mix, the mixed liquor making Paecilomyces lilacinus and chitin continues cultivation 10 days more than and namely obtains this preparation.Described chitin can adopt the mode of chitin solution to add culture fluid.
Embodiment 2
Prevent and treat a preparation method for the biologic product of vegetables nematodosis, for first Paecilomyces lilacinus being passed through medium culture, adding again after cultivating maturation after chitin carries out Mixed culture and obtaining.
Wherein, first obtained described medium is: get raw material beef extract 5 kilograms, peptone 3 kilograms, sugar 100 kilograms, 10 kilograms, phosphorous acid, 10 kilograms, potassium; Above-mentioned raw materials is put into fermentation tank and add water to 1000 kilograms, pH value is adjusted to 7, is warmed to 120 DEG C and keeps 24 hours, cool the temperature to 32 DEG C afterwards.Afterwards described Paecilomyces lilacinus is dropped into fermentation tank, described Paecilomyces lilacinus drops into fermentation tank, and stir oxygenation and cultivate, the cultivation temperature that described Paecilomyces lilacinus is cultivated is 32 DEG C, pH value is 7, cultivate and cultivate ripe culture fluid in 7 days, afterwards chitin mix with the ratio of culture fluid according to weight ratio 5: 5, namely the mixed liquor continuation cultivation of making Paecilomyces lilacinus and chitin is obtained this preparation in 12 days.Described chitin adds culture fluid in the mode of chitin solution.
Embodiment 3
Prevent and treat a preparation method for the biologic product of vegetables nematodosis, for first Paecilomyces lilacinus being passed through medium culture, adding again after cultivating maturation after chitin carries out Mixed culture and obtaining.
Wherein, first obtained described medium is: get raw material beef extract 6 kilograms, peptone 4 kilograms, sugar 200 kilograms, 20 kilograms, phosphorous acid, 20 kilograms, potassium; Above-mentioned raw materials is put into fermentation tank and add water to 1000 kilograms, pH value is adjusted to 7, be warmed to 130 DEG C and keep 28 hours, cool the temperature to 30 DEG C afterwards.Afterwards described Paecilomyces lilacinus is dropped into fermentation tank, described Paecilomyces lilacinus drops into fermentation tank, and stir oxygenation and cultivate, the cultivation temperature that described Paecilomyces lilacinus is cultivated is 30 DEG C, pH value is 7, cultivate and cultivate ripe culture fluid in 5 days, afterwards chitin mix with the ratio of culture fluid according to weight ratio 5: 5, namely the mixed liquor continuation cultivation of making Paecilomyces lilacinus and chitin is obtained this preparation in 11 days.Described chitin adds culture fluid in the mode of chitin solution.
The biologic product of the control vegetables nematodosis using this kind of preparation method to produce has following effect:
1, strengthen fertility: can nearly half consumption of substituted chemistry base manure, in production, Shaoshi fertilizer, significantly reduces production cost;
2, volume increase good harvest: there is spontaneous growth and regulate plain PGPR effect, can stratification, grow, bloom, result, make term in advance, raising output, increases income;
3, oil recovery enhancement: make vegetables fresh and tender, melon and fruit is sharp and clear, fragrant and sweet delicious, bright in colour, freshness increases, and improve quality of agricultural product, valency height is in great demand again;
4, save manually: can remove soil compaction, acidifying, root is prosperous healthy and strong, and cauline leaf stalwartness is anosis, reduce agricultural chemicals demand and cost of labor, protection peasant is healthy;
5, improve effects: the breeding that can promote Soil Probiotics group, reduces continuous cropping obstacle, increases land-use utility;
6, reduce or remit residual hazard: can strengthen that crop is degeneration-resistant, resistance against diseases, reduce Pesticide use amount, degraded agricultural product medicine is residual, pursues organic, green non-pollution standard;
7, improve soil property: can provide organic acid, decompose insoluble salt based component in soil, releasing is hardened, and reduces alkali, and soil strong acid is released in punching, removes acidifying and hardens and alkali problem;
8, nematode is eliminated: by the cooperation of actinomycetes and natural goods, materials and equipments, after weakening epidermis, then nematode can be eliminated through infecting;
9, to contend with harmful bacterium: soil ecology balance can be recovered, prevent the excessive growth of germ, avoid various disease and rotten;
10, worm's ovum is destroyed: worm's ovum shell in soil can be dissolved by organic matter, stop the chance that insect is produced a male heir to continue the family line.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made and also should be considered as protection scope of the present invention.

Claims (6)

1. prevent and treat a preparation method for the biologic product of vegetables nematodosis, it is characterized in that: first by the cultivation of Paecilomyces lilacinus by medium, after cultivating maturation, add the biologic product obtaining control vegetables nematodosis after chitin carries out Mixed culture again.
2. a kind of preparation method preventing and treating the biologic product of vegetables nematodosis according to claim 1, it is characterized in that: the cultural method of described medium is: get raw material beef extract 5-6 part by weight, peptone 3-4 part, sugared 100-200 part, phosphorous acid 10-20 part, potassium 10-20 part; Above-mentioned raw materials is put into fermentation tank and add water to 1000 parts, pH value is adjusted to 6.5-7.5, be warmed to 110-130 DEG C and keep 20-28 hour, cool the temperature to 30-34 DEG C afterwards.
3. a kind of preparation method preventing and treating the biologic product of vegetables nematodosis according to claim 1, is characterized in that: described medium, comprises the raw material of following weight proportion: raw material beef extract 5-6 part, peptone 3-4 part, sugar 100-200 part, phosphorous acid 10-20 part, potassium 10-20 part.
4. a kind of preparation method preventing and treating the biologic product of vegetables nematodosis according to claim 1, it is characterized in that: described Paecilomyces lilacinus drops into fermentation tank, and stir oxygenation and cultivate, cultivation temperature is 28-32 DEG C, pH value is 6.5-7.5, cultivate and cultivate ripe culture fluid in 5-7 days, afterwards by chitin and culture fluid according to weight ratio 4-6: 6-4 ratio mix, namely the mixed liquor continuation cultivation more than 10 days making Paecilomyces lilacinus and chitin obtains this preparation.
5. a kind of preparation method preventing and treating the biologic product of vegetables nematodosis according to claim 1, is characterized in that: described chitin adds culture fluid in the mode of chitin solution.
6. a kind of preparation method preventing and treating the biologic product of vegetables nematodosis according to claim 1, is characterized in that: described chitin mixes with the ratio of culture fluid according to weight ratio 5: 5.
CN201610022029.9A 2016-01-07 2016-01-07 Preparation method of biological preparation for preparing and curing vegetable nematode disease Pending CN105494448A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105901038A (en) * 2016-05-23 2016-08-31 方有厉 Plant insecticide for preventing and treating vegetable root nematodes and preparation method thereof
CN111903714A (en) * 2019-05-10 2020-11-10 漯河市盛粮生物科技有限公司 Preparation method of microbial agent for preventing and treating wheat root nematodes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1439270A (en) * 2002-02-20 2003-09-03 汪洪 Preparing method for lilacinin against nematoda eggs
CN101422168A (en) * 2008-12-23 2009-05-06 北京市农林科学院 New method for controlling rot knot nematode disease of protected vegetable
CN104719345A (en) * 2015-03-31 2015-06-24 青州伟格生物科技有限公司 Preparation method of biological preparation for preventing and controlling vegetable nematode disease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1439270A (en) * 2002-02-20 2003-09-03 汪洪 Preparing method for lilacinin against nematoda eggs
CN101422168A (en) * 2008-12-23 2009-05-06 北京市农林科学院 New method for controlling rot knot nematode disease of protected vegetable
CN104719345A (en) * 2015-03-31 2015-06-24 青州伟格生物科技有限公司 Preparation method of biological preparation for preventing and controlling vegetable nematode disease

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105901038A (en) * 2016-05-23 2016-08-31 方有厉 Plant insecticide for preventing and treating vegetable root nematodes and preparation method thereof
CN105901038B (en) * 2016-05-23 2018-09-14 方有历 Prevent the vegetable insecticide and preparation method thereof of vegetable root nematode
CN111903714A (en) * 2019-05-10 2020-11-10 漯河市盛粮生物科技有限公司 Preparation method of microbial agent for preventing and treating wheat root nematodes

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Application publication date: 20160420