CN110896964A - Seed dressing agent without pesticide residue and preparation method thereof - Google Patents
Seed dressing agent without pesticide residue and preparation method thereof Download PDFInfo
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- CN110896964A CN110896964A CN201911321624.2A CN201911321624A CN110896964A CN 110896964 A CN110896964 A CN 110896964A CN 201911321624 A CN201911321624 A CN 201911321624A CN 110896964 A CN110896964 A CN 110896964A
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Abstract
The invention provides a seed dressing agent without pesticide residue and a preparation method thereof, belonging to the field of biological products, wherein the seed dressing agent comprises 25-30 parts of metarhizium anisopliae, 25-30 parts of beauveria bassiana, 3000 parts of animal protein 2000-; the animal protein and the brown sugar provide nutrition for the beauveria bassiana, the metarhizium anisopliae and the microzyme in the fermentation process, some toxins can be generated in the fermentation process of the metarhizium anisopliae, and the metarhizium anisopliae has high insecticidal activity, and in addition, hypha and spores of the metarhizium anisopliae have high bactericidal activity; the beauveria bassiana can generate compounds such as toxins, hydrolytic enzymes, organic acids and the like in the fermentation process, can resist insects, and can also generate beauvericin, oosporine and calcium oxalate crystals, the substances can cause insect poisoning and disturb metabolism to cause death, in addition, the beauveria bassiana mainly infects host cells and forms specific sexual organisms after symbiosis with the host cells to play a drug effect, the beauveria bassiana can also induce apoptosis and the like, and can well prevent and treat underground insect pests.
Description
Technical Field
The invention relates to the technical field of biological products, in particular to a seed dressing agent without pesticide residues and a preparation method thereof.
Background
Seed dressing is a method for treating plant seedlings, and in practical application, the seed dressing in a narrow sense and a broad sense is a method for uniformly coating a layer of pesticide or trace fertilizer on the surface of seeds by mixing the seeds with the pesticide or the trace fertilizer; the seed dressing in a broad sense also comprises seed soaking, seed smothering, root soaking, root dipping and other seedling treatment methods besides the above contents.
The objects for dressing seeds are roots of seeds and fungus wood, and root tubers, bulbs, cuttings and the like which have the same effect with the seeds.
The seed dressing method can be divided into two methods of dry mixing and wet mixing. The powder or wettable powder can be mixed with seeds by dry mixing, namely, the sample powder is put into a seed mixer or other apparatuses and is directly mixed with the seeds uniformly. The wet mixing is to add water into the seed-dressing agent according to a certain proportion to prepare a liquid medicine, then spray the liquid medicine on the seeds, mix with a wooden shovel or a shovel to make the surface of the seeds uniformly coated with a layer of medicine film, and then dry the seeds in the air to obtain the seeds. The micro fertilizer for seed dressing is prepared through compounding water soluble trace element fertilizer into solution of certain concentration, spraying the solution onto seed, stirring to make the seed coated with one layer of solution, piling for 3-4 hr, and drying in the air for seeding.
The purpose of dressing seeds is to kill pests, sterilize and strengthen seedlings in general. The pesticide is used for dressing seeds to eliminate pests and pathogens carried on the surfaces and in the seeds, protect seedlings from being damaged by the pests in soil, and absorb the pesticide by seed bacteria to be guided to the overground part to prevent the pests from being damaged. The micro-fertilizer is used for dressing seeds, so that the defect that the soil can not meet the requirement of crops on trace elements can be overcome, and the cultivation of strong seedlings is promoted. Of course, the functions of pesticide dressing and micro-fertilizer dressing are not completely separated, and the pesticide dressing and the micro-fertilizer dressing have a very close relationship. The seed dressing by using pesticide is used for preventing and controlling plant diseases and insect pests, and is also an important measure for culturing strong seedlings; the strong bacteria and strong plants formed by micro-fertilizer seed dressing are also beneficial to improving the disease and insect resistance of the plants.
The patent of application No. CN200710185459.3 discloses a seed dressing agent and a preparation method thereof, the seed dressing agent is a seed dressing agent formed by combining active ingredients of abamectin, thiram and carbendazim, the weight ratio of the active ingredients is that the abamectin, the thiram and the carbendazim are 0.1-0.5: 15-35: 10-30, and a proper amount of absorption sustained release agent, emulsifier and auxiliary agent are added. The invention also provides a preparation method of the seed dressing agent. The seed dressing agent has strong complementarity among medicaments and remarkable synergism, can prevent and control various soil-borne and seed-borne diseases, and can efficiently kill underground pests such as root-knot nematodes, root-miners and the like. After the pesticide is used, the plant has developed root system, strong growth, strong disease resistance and obvious yield increase.
The patent of application No. CN201510774825.3 discloses a broom corn millet seed dressing agent, which comprises active ingredients, wherein the active ingredients comprise 12-18% of thiophanate-methyl, 1.5-6% of triadimefon, 0.25-1% of imidacloprid, 0.25-1% of methomyl and 2-8% of monopotassium phosphate, and the total mass of the broomrape dressing agent is respectively accounted by the triadimefon, the benazolin, the methomyl and the monopotassium phosphate. The millet seed dressing agent can effectively prevent millet smut, and simultaneously provides nutrient substances in the seed germination stage, so that the millet seedling stage is promoted to be uniform and strong, the agricultural character of the millet is improved, and the yield of the millet is increased.
Although the two patents can effectively prevent and treat underground insect pests and diseases, improve the agronomic characters of crops and increase the content, the two patents have the common defect that a part of pesticides directly or indirectly exist in crops and soil due to the fact that the seed dressing agent contains pesticides such as abamectin, thiram, carbendazim, thiophanate-methyl, triazolone, imidacloprid, methomyl and the like, the health of animals, plants and human beings is seriously threatened, and meanwhile, environmental pollution is caused.
Disclosure of Invention
Aiming at the defects that the existing seed dressing agent has pesticide residues, seriously influences the health of animals, plants and human beings and causes environmental pollution, the invention provides a seed dressing agent without pesticide residues and a preparation method thereof.
The seed dressing agent without pesticide residue comprises, by weight, 25-30 parts of metarhizium anisopliae, 25-30 parts of beauveria bassiana, 3000 parts of animal protein 2000-.
According to the technical scheme, the metarhizium anisopliae is very similar to penicillium in classification status, the metarhizium anisopliae grows well on a potato culture medium, a white bacterial colony is formed firstly, hypha branches are interwoven after 2-3 days to form a conidiophore, penicillium-shaped clustered conidia are grown on the conidiophore, the conidia are initially white, and the conidia are green or dark green when slowly maturing. The green muscardine fungus is broad-spectrum insect pathogenic bacteria, is harmless to people and livestock, is safe to natural enemy insects, and does not pollute the environment; beauveria bassiana is an entomogenous fungus of ascomycetes, the main species comprises Beauveria bassiana, Beauveria bassiana and the like, conidia are usually generated through asexual propagation, hypha has cross branches, the Beauveria bassiana mainly infects host cells and forms a characteristic sexual type organism after symbiosis with the host cells so as to play the drug effect, and the Beauveria bassiana can also induce apoptosis and the like; the yeast is a unicellular fungus, belongs to facultative anaerobes, contains rich substances such as protein, B vitamins, amino acid and the like, and can generate a large amount of energy for the utilization of metarhizium anisopliae and beauveria bassiana by fermentation under the aerobic condition; the animal protein and brown sugar provide nutrition for beauveria bassiana, metarhizium anisopliae and yeast in the fermentation process, toxins such as DtxA, DtxB2 and DtrxE can be generated in the fermentation process of the metarhizium bassiana, and the toxins have high insecticidal activity, and hypha and spores of the metarhizium bassiana in China also have high bactericidal activity; the beauveria bassiana can generate compounds such as toxins, hydrolytic enzymes, organic acids and the like in the fermentation process, can resist insects, can also generate beauvericin (non-ribosomal polypeptide toxin), oosporine (benzoquinone toxin) and calcium oxalate crystals, can cause insect poisoning, disturb metabolism and cause death, and can play a drug effect mainly by infecting host cells and forming specific sexual organisms after symbiosis with the host cells.
Preferably, the seed dressing agent comprises 28 parts of metarhizium anisopliae, 28 parts of beauveria bassiana, 2500 parts of animal protein, 250 parts of brown sugar and 18 parts of yeast in parts by weight.
Preferably, the paint also comprises water, and the water comprises 25000 and 28000 parts by weight.
More preferably, 26500 parts of water.
Preferably, the animal protein comprises one or more of fish protein, earthworm, yellow worm powder, oyster powder, shrimp powder and silkworm chrysalis powder.
Preferably, the spore content of Metarrhizium anisopliae is not less than 100 hundred million/gram.
Preferably, the spore content of beauveria bassiana is not less than 50 hundred million/gram.
Preferably, the number of spores of the yeast is not less than 50 hundred million/g.
A preparation method of a seed dressing agent without pesticide residues comprises the following steps of adding green muscardine fungi, white muscardine fungi, animal protein, brown sugar and saccharomycetes into a fermentation tank according to the parts by weight, adding water, stirring uniformly, and carrying out aerobic fermentation at the temperature of 22-27 ℃ for 40-60 hours to obtain the seed dressing agent.
Preferably, the fermentation is carried out for 48 hours under the temperature of 25 ℃.
The seed dressing agent can be used for dressing seeds of peanuts, wheat, broad beans, corns, potatoes and the like, and preventing and controlling underground pests such as scarab beetles, weevils, wireworms, moth butterfly larvae, stinkbugs, aphids and the like.
In the technical scheme of the application, the metarhizium anisopliae, the beauveria bassiana and the yeast are all from the market.
Compared with the prior art, the invention has the beneficial effects that:
(1) the green muscardine fungi, the white muscardine fungi and the microzyme have reasonable proportion to form beneficial microorganism compound bacteria, can well prevent and treat underground insect pests, have no pathogenic effect on human and livestock, belong to weak allergic reaction sources, and do not cause mutation, carcinogenesis and teratogenesis;
(2) the animal protein and brown sugar provide nutrition for beauveria bassiana, metarhizium anisopliae and yeast in the fermentation process, toxins such as DtxA, DtxB2 and DtrxE can be generated in the fermentation process of the metarhizium bassiana, and the toxins have high insecticidal activity, and hypha and spores of the metarhizium bassiana in China also have high bactericidal activity; the beauveria bassiana can generate compounds such as toxins, hydrolytic enzymes, organic acids and the like in the fermentation process, can resist insects, and can also generate beauvericin (non-ribosomal polypeptide toxin), oosporine (benzoquinone toxoid) and calcium oxalate crystals, which can cause insect poisoning and disturb metabolism to death, in addition, the beauveria bassiana mainly infects host cells to form a specific sexual organism after symbiosis with the host cells so as to play a drug effect, and the beauveria bassiana can also induce apoptosis and the like;
(3) the yeast is a unicellular fungus, belongs to facultative anaerobes, contains rich substances such as protein, B vitamins, amino acid and the like, and can generate a large amount of energy for the utilization of metarhizium anisopliae and beauveria bassiana by fermentation under the aerobic condition;
(4) the method has the advantages of easy obtainment of various microorganisms, simple fermentation method, short period, capability of dressing seeds with various crop seeds and preventing and controlling soil insects, and wide application range.
(5) The prevention and control rate of various soil insects is high and reaches about 85 percent.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following detailed description of the present invention is provided with reference to specific embodiments.
Example 1
A seed dressing agent without pesticide residue comprises, by weight, 25 parts of metarhizium anisopliae, 25 parts of beauveria bassiana, 2000 parts of animal protein, 200 parts of brown sugar and 15 parts of microzyme; 25000 parts of water by weight; the animal protein comprises one or more of fish protein, earthworm, yellow worm powder, oyster powder, shrimp powder and silkworm pupa powder; the spore content of the metarhizium anisopliae is not less than 100 hundred million/g; the spore content of beauveria bassiana is not less than 50 hundred million/g; the number of spores of the yeast is not less than 50 hundred million/g.
A preparation method of a seed dressing agent without pesticide residues comprises the following steps of adding green muscardine fungus, white muscardine fungus, animal protein, brown sugar and saccharomycetes into a fermentation tank according to the parts by weight, adding water, stirring uniformly, and carrying out aerobic fermentation for 60 hours at 22 ℃.
Example 2
A seed dressing agent without pesticide residue comprises, by weight, 28 parts of Metarrhizium anisopliae, 28 parts of Beauveria bassiana, 2500 parts of animal protein, 250 parts of brown sugar, 18 parts of microzyme and 26500 parts of water; the animal protein comprises one or more of fish protein, earthworm, yellow worm powder, oyster powder, shrimp powder and silkworm pupa powder; the spore content of the metarhizium anisopliae is not less than 100 hundred million/g; the spore content of beauveria bassiana is not less than 50 hundred million/g; the number of spores of the yeast is not less than 50 hundred million/g.
A preparation method of a seed dressing agent without pesticide residues comprises the following steps of adding green muscardine fungi, white muscardine fungi, animal protein, brown sugar and saccharomycetes into a fermentation tank according to the parts by weight, adding water, stirring uniformly, and carrying out aerobic fermentation for 48 hours at 25 ℃ to obtain the seed dressing agent.
Example 3
A seed dressing agent without pesticide residue comprises, by weight, 30 parts of metarhizium anisopliae, 30 parts of beauveria bassiana, 3000 parts of animal protein, 300 parts of brown sugar and 20 parts of microzyme; 28000 parts of water by weight; the animal protein comprises one or more of fish protein, earthworm, yellow worm powder, oyster powder, shrimp powder and silkworm pupa powder; the spore content of the metarhizium anisopliae is not less than 100 hundred million/g; the spore content of beauveria bassiana is not less than 50 hundred million/g; the number of spores of the yeast is not less than 50 hundred million/g.
A preparation method of a seed dressing agent without pesticide residues comprises the following steps of adding green muscardine fungi, white muscardine fungi, animal protein, brown sugar and saccharomycetes into a fermentation tank according to the parts by weight, adding water, stirring uniformly, and then carrying out aerobic fermentation for 40 hours at the temperature of 27 ℃ to obtain the seed dressing agent.
Test example 1
Mixing the seed dressing agent prepared in the embodiment 2 with wheat seeds, wherein the ratio of the seed dressing agent to the wheat seeds is 1: 50 according to the volume ratio, uniformly stirring, and naturally airing to sow; meanwhile, a control group is set, the control group is formed by equal amounts of sterile water and wheat seeds, after seed dressing, sowing is carried out after the seeds are naturally dried, and when the wheat seedlings grow to be 15cm high, the insect pest rate is counted, and the table 1 shows.
TABLE 1 Pest infestation rates of wheat soil pests
As shown in Table 1, the seed dressing agent in the embodiment 2 of the application has the advantages that the insect pest rate of wheat is 3% after seed dressing, the control group is as high as 15%, the release efficiency is 80%, and the control effect is good.
Test example 2
Mixing the seed dressing agent prepared in the embodiment 1 with peanut seeds, dressing the seeds according to the volume ratio of 1: 50 of the seed dressing agent to the peanut seeds, stirring uniformly, and naturally airing to sow; meanwhile, a control group is set, the control group is composed of the same amount of sterile water and peanut seeds, after seed dressing, the peanut seeds are naturally aired and sowed, and when the peanut seedlings grow to 10cm high, the insect pest rate is counted, and the result is shown in table 1.
TABLE 2 Pest infestation rates of peanut soil pests
As shown in Table 2, the seed dressing agent in the embodiment 1 of the application has the advantages that the pest damage rate of peanuts after seed dressing is 3%, the pest damage rate of the control group is as high as 20%, the seed release efficiency is 85%, and the control effect is good.
The above-mentioned embodiments only express the specific embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for those skilled in the art, without departing from the technical idea of the present application, several changes and modifications can be made, which are all within the protection scope of the present application.
Claims (10)
1. A seed dressing agent without pesticide residue is characterized in that: the seed dressing agent comprises 25-30 parts of metarhizium anisopliae, 25-30 parts of beauveria bassiana, 3000 parts of animal protein 2000-
And 20 parts.
2. A seed dressing agent free of pesticide residue according to claim 1, characterized in that: the seed dressing agent comprises, by weight, 28 parts of metarhizium anisopliae, 28 parts of beauveria bassiana, 2500 parts of animal protein, 250 parts of brown sugar and 18 parts of saccharomycetes.
3. A seed dressing agent free of pesticide residue according to claim 1 or 2, characterized in that: and water 25000 and 28000 parts by weight.
4. A seed dressing agent free of pesticide residue according to claim 3, characterized in that: 26500 parts of water.
5. A seed dressing agent free of pesticide residue according to claim 1 or 2, characterized in that: the animal protein comprises one or more of fish protein, Lumbricus, yellow worm powder, Concha Ostreae powder, shrimp powder, and pupa Bombycis powder.
6. A seed dressing agent free of pesticide residue according to claim 1 or 2, characterized in that: the spore content of Metarrhizium anisopliae is not less than 100 hundred million/g.
7. A seed dressing agent free of pesticide residue according to claim 1 or 2, characterized in that: the spore content of beauveria bassiana is not less than 50 hundred million/g.
8. A seed dressing agent free of pesticide residue according to claim 1 or 2, characterized in that: the number of spores of the yeast is not less than 50 hundred million/g.
9. A method for preparing a seed dressing agent free of pesticide residues according to any one of claims 1 to 8, characterized in that: comprises the following steps of adding the green muscardine fungi, the white muscardine fungi, the animal protein, the brown sugar and the microzyme into a fermentation tank according to the weight parts, adding water, stirring uniformly, and then carrying out aerobic fermentation for 40-60 hours at the temperature of 22-27 ℃ to obtain the green muscardine fungus.
10. A method for preparing a seed dressing agent without pesticide residue according to any of claim 9, characterized in that: performing aerobic fermentation at 25 deg.C for 48 hr.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112262723A (en) * | 2020-11-05 | 2021-01-26 | 青海省农林科学院 | Whole-course biological prevention and control method for diseases and insect pests of broad beans |
| CN112400625A (en) * | 2020-11-19 | 2021-02-26 | 河北师范大学 | Green land plant fertilization management method |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0748216A (en) * | 1993-08-04 | 1995-02-21 | Fuji Giken Kk | Material for suppressing soil vermin |
| CN1395839A (en) * | 2002-05-13 | 2003-02-12 | 中国农业科学院生物防治研究所 | Preparing process and usage of muscardine insecticide |
| CN1463604A (en) * | 2002-06-03 | 2003-12-31 | 杨爱民 | Pest control agent comprising multiple micro-organism |
| CN101338279A (en) * | 2008-05-07 | 2009-01-07 | 浙江大学 | Hyphomycetes mite-killing preparation |
| CN101671212A (en) * | 2009-09-28 | 2010-03-17 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN104351260A (en) * | 2014-10-14 | 2015-02-18 | 深圳文科园林股份有限公司 | Biological pesticide for killing aphids and preparation method for biological pesticide |
| CN107372615A (en) * | 2017-07-27 | 2017-11-24 | 吉林省利泽生物科技有限公司 | A kind of seed dressing and its preparation method and application |
| CN108330071A (en) * | 2017-01-19 | 2018-07-27 | 中国科学院上海生命科学研究院 | Have Strain of Beauveria bassiana and its control of insect application of insect gut infection effect |
| WO2019229624A1 (en) * | 2018-05-27 | 2019-12-05 | The State Of Israel, Ministry Of Agriculture & Rural Development Agricultural Research Organization | Single cell encapsulation via pickering emulsion for bio-pesticides application |
-
2019
- 2019-12-19 CN CN201911321624.2A patent/CN110896964A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0748216A (en) * | 1993-08-04 | 1995-02-21 | Fuji Giken Kk | Material for suppressing soil vermin |
| CN1395839A (en) * | 2002-05-13 | 2003-02-12 | 中国农业科学院生物防治研究所 | Preparing process and usage of muscardine insecticide |
| CN1463604A (en) * | 2002-06-03 | 2003-12-31 | 杨爱民 | Pest control agent comprising multiple micro-organism |
| CN101338279A (en) * | 2008-05-07 | 2009-01-07 | 浙江大学 | Hyphomycetes mite-killing preparation |
| CN101671212A (en) * | 2009-09-28 | 2010-03-17 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN104351260A (en) * | 2014-10-14 | 2015-02-18 | 深圳文科园林股份有限公司 | Biological pesticide for killing aphids and preparation method for biological pesticide |
| CN108330071A (en) * | 2017-01-19 | 2018-07-27 | 中国科学院上海生命科学研究院 | Have Strain of Beauveria bassiana and its control of insect application of insect gut infection effect |
| CN107372615A (en) * | 2017-07-27 | 2017-11-24 | 吉林省利泽生物科技有限公司 | A kind of seed dressing and its preparation method and application |
| WO2019229624A1 (en) * | 2018-05-27 | 2019-12-05 | The State Of Israel, Ministry Of Agriculture & Rural Development Agricultural Research Organization | Single cell encapsulation via pickering emulsion for bio-pesticides application |
Non-Patent Citations (2)
| Title |
|---|
| 洪坚平 等编著: "《农业微生物资源的开发与利用》", 31 August 2000, 中国林业出版社 * |
| 牛天贵 等编著: "《食用菌消费指南》", 31 May 2000, 农村读物出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112262723A (en) * | 2020-11-05 | 2021-01-26 | 青海省农林科学院 | Whole-course biological prevention and control method for diseases and insect pests of broad beans |
| CN112262723B (en) * | 2020-11-05 | 2023-06-23 | 青海省农林科学院 | Whole-course biological prevention and control method for broad bean diseases and insect pests |
| CN112400625A (en) * | 2020-11-19 | 2021-02-26 | 河北师范大学 | Green land plant fertilization management method |
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Application publication date: 20200324 |