CN104351260A - Biological pesticide for killing aphids and preparation method for biological pesticide - Google Patents
Biological pesticide for killing aphids and preparation method for biological pesticide Download PDFInfo
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- CN104351260A CN104351260A CN201410556978.6A CN201410556978A CN104351260A CN 104351260 A CN104351260 A CN 104351260A CN 201410556978 A CN201410556978 A CN 201410556978A CN 104351260 A CN104351260 A CN 104351260A
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Abstract
The invention belongs to the technical field of biology, and particularly relates to a biological pesticide for killing aphids and a preparation method for the biological pesticide. The biological pesticide comprises the main components of nontoxic and harmless cinnamomum camphora steam and leaf extract (camphor), plant ash leachate, beauveria bassiana, metarrhizium anisopliae, yeasts and glucose, so that insecticidal effects of the biological pesticide are greatly improved, and organic nutrients are provided for plants to promote the growth of the plants. The application of the biological pesticide is relatively specific, and the biological pesticide is mainly used for damage caused by aphids to various plants, and also has unobvious prevention and control effects on other insect damage. The biological pesticide has the characteristics of nontoxicity, high efficiency, no contamination, no pollution and no residues, is an important means for realizing green ecology, and has broad development prospect.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of biopesticide and compound method of killing aphids.
Background technology
Aphid Species is various, and the world known about more than 4700 plants, and China just about has 1100 kinds.Its fertility is extremely strong, can breed 20-30 generation every year, and often huge for reproductive number.China, as large agricultural country, is extensively subject to the infringement of aphid simultaneously.But along with the development of agricultural industry, the hope green consumption that people are urgent again, the requirement particularly for the primary industry such as fruit, vegetables is strict especially.Many just because of Aphid Species, so the vegetation type of harm is also many.Current country carries out Green China, certainly also comprise urban afforestation, but aphid endangers for the view plant of urban afforestation equally huge.At present, the agricultural chemicals that people use is commonly chemical pesticide.Although the insecticidal effect of most phosphoramidite chemical agricultural chemicals is high, but easily cause more serious consequence in the normal region of chemical pesticide that uses, such as chemical pesticide residual harm people and animals, the poisoning that strong cytotoxic chemical agricultural chemicals causes, the pest resistance to insecticide often using chemical pesticide to cause strengthens.The variety of problems brought based on agriculturally using chemical pesticide, people start urgent to wish the not only material benefit of a kind of economy, effective nonhazardous but also the agricultural chemicals of environmental protection, improve agricultural product security sexual valence value.
Summary of the invention
The object of the present invention is to provide that a kind of insecticidal effect is high, with low cost, biopesticide killing aphids technology to environment and person poultry harmless.Use the method can greatly improve extensively by the agricultural product income effect of aphid insect damage, and ensure to reduce agriculturally to the pollution of environment.
The technical thought that the present invention takes is: by utilizing the symbiotic relation between aphid and ant, adds nontoxic camphor tree stem-leaf extract (camphor) and drive away ant in agricultural chemicals, makes it to remove symbiotic relation thus protection natural enemy of aphid; Reducing aphid immunocompetence by adding ash leachate destruction aphid surface protection tissue (triglyceride) in agricultural chemicals, providing nutrient to plant growth, Promoting plant growth simultaneously; By utilizing white muscardine fungi, parasitic relation between green muscardine fungus and aphid, in agricultural chemicals, add selectivity strong and to the broken ring aphid body tissue of the entomophagous parasite bacterium (white muscardine fungi, green muscardine fungus) of person poultry harmless, and make aphid dead; By utilizing saccharomycetic respiration principle, in agricultural chemicals, adding saccharomycete and a small amount of glucose, making saccharomycete decompose the honeydew of dead aphid body tissue and aphid secretion.The parts by weight wherein preparing each raw material needed for killing aphids biopesticide are: camphor 3-4 part, ash leachate 10-15 part, white muscardine fungi powder 0.2-0.3 part, green muscardine fungus powder 0.3-0.4 part, yeast powder 0.05-0.08 part, glucose pulvis 5-8 part.
A biopesticide compound method for killing aphids, comprises the following steps:
(1) the camphor tree branches and leaves in period short of rain in autumn are got, put into mortar after shredding to grind, steam distilled method is used after abundant grinding, the steam of camphor and camphor oil that has obtained is entered the dish type cooler of many rings again through wireway, the mixture of camphor and camphor oil and water can be obtained, by leaving standstill rear layering, remove camphor and the camphor oil mixture of gained after moisture, entered multiple method of steaming again and collected distillate under the condition of 155-200 DEG C, the crystalline solid of separating out after the distillate now collected cooling was crystallized camphor;
(2) get ash fully to mix with water, mixed weight ratio is 1: 1, and ash, after entering circadian immersion, filters gained supernatant and is leachate;
(3) white muscardine fungi powder, green muscardine fungus powder and yeast powder is taken by the weight of each component, mix adding pulvis without the white muscardine fungi powder activated, green muscardine fungus powder and yeast powder with biopesticide, obtain Mixed Microbes pulvis I, guarantee under the dry condition, avoid bacterial classification at medication front activating;
(4) take a small amount of glucose as above-mentioned actication of culture basic material by the weight of each component, glucose is made pulvis;
(5) crystallized camphor of gained, ash leachate, Mixed Microbes pulvis I, glucose pulvis are stirred by after the mixing of each component weight.Parts by weight are: camphor 3-4 part, ash leachate 10-15 part, white muscardine fungi powder 0.2-0.3 part, green muscardine fungus powder 0.3-0.4 part, yeast powder 0.05-0.08 part, glucose pulvis 5-8 part.
White muscardine fungi (Beauveria bassiana) in the present invention is stored in China typical culture collection center, and preservation bacterium number is CGMCC 3.03574.Green muscardine fungus (Metarhizium anisopliae) is stored in China General Microbiological Bacterial Strains Managing center, and preservation bacterium number is CGMCC 3.03676.Saccharomycete (Trichosporon capitatum) is stored in China typical culture collection center, and preservation bacterium number is CCTCC AY93140.Above bacterial classification is all bought in Hunan Yuyuan Biology Technology Co., Ltd.
Advantage of the present invention is:
1, avirulence: the camphor that the present invention adds, ash leachate, glucose pulvis all to people and animals' nonhazardous effect, not only substantially increase the insecticidal effect of medicine, but also provide nutrient, Promoting plant growth for plant.
2, nonstaining property: the camphor that the present invention adds is also volatile while playing desinsection anthelmintic action in the environment, can not cause harmful effect to environment; Ash leachate is alkalescent mixture, and its main component is potash, agriculturally not only can be used as concentrated fertilizer but also also having the effect of preventing damage by disease and insect, also can not produce harmful effect to environment; Glucose pulvis main component is glucose, for the respiratory raw material of most of microbe, the a small amount of glucose added in this agricultural chemicals is as saccharomycetic activation basic material, and after saccharomycete activation, the end product of decomposition glucose is carbonic acid gas and water, on environment also without impact; The Mixed Microbes added is the strong microorganism of selectivity, on environment also without impact.
3, noresidue: each component of agricultural chemicals, after medication, is all decomposed by the microorganisms or is absorbed by plants, without secondary products, microorganism when without also can natural death when parasitic source, to plant noresidue.
4, high efficiency: the present invention kills aphid mainly through the parasitization of white muscardine fungi and green muscardine fungus, belong to Biocidal method, meanwhile camphorating, mentioned microorganism can be assisted to colonize in smoothly in aphid body after ash leachate, thus reach the object of efficient killing aphids.
Embodiment
1, a kind of biopesticide compound method of killing aphids mainly comprises the following steps:
(1) the camphor tree branches and leaves in period short of rain in autumn are got, put into mortar after shredding to grind, steam distilled method is used after abundant grinding, the steam of camphor and camphor oil that has obtained is entered the dish type cooler of many rings again through wireway, the mixture of camphor and camphor oil and water can be obtained, by leaving standstill rear layering, remove camphor and the camphor oil mixture of gained after moisture, entered multiple method of steaming again and collected distillate under the condition of 155-200 DEG C, the crystalline solid of separating out after the distillate now collected cooling was crystallized camphor;
(2) get ash fully to mix with water, mixed weight ratio is 1: 1, and ash, after entering circadian immersion, filters gained supernatant and is leachate;
(3) white muscardine fungi powder, green muscardine fungus powder and yeast powder is taken by the weight of each component, mix adding pulvis without the white muscardine fungi powder activated, green muscardine fungus powder and yeast powder with biopesticide, obtain Mixed Microbes pulvis I, guarantee under the dry condition, avoid bacterial classification at medication front activating;
(4) take a small amount of glucose as above-mentioned actication of culture basic material by the weight of each component, glucose is made pulvis;
(5) by camphor 3-4 part, ash leachate 10-15 part, Mixed Microbes pulvis I (white muscardine fungi powder 0.2-0.3 part, green muscardine fungus powder 0.3-0.4 part, yeast powder 0.05-0.08 part), stirs after the mixing of glucose pulvis 5-8 part.
Following examples are all carrying out according to embodiment of the present invention, and aphid insect damage prevention and treatment range includes but not limited to following examples.
Embodiment 1
On July 20th, 2014 chooses rose of Sharon aphid as experiment material, and the healthy aphid 500 of picking, isolates plant.Obtain 1000 times of liquid with after pulvis 10g of the present invention, liquor 10g thin up, within every two weeks, use the present invention once.Experimental result: after one month, rose of Sharon children browse haunts without ant, and Aphed population obviously reduces, and quantity is 24; Observe rose of Sharon growing state without exception; Aphid lethality is 95.2%.
Embodiment 2
On July 16th, 2014 chooses photinia glabra aphid as experiment material, and the healthy aphid 500 of picking, isolates plant.Obtain 1000 times of liquid with after pulvis 10g of the present invention, liquor 10g thin up, within every two weeks, use the present invention once.Experimental result: after one month, photinia glabra children browse haunts without ant, and Aphed population obviously reduces, and quantity is 21; Observe photinia glabra growing state without exception; Aphid lethality is 95.8%.
Embodiment 3
On July 22nd, 2014 chooses crape myrtle aphid as experiment material, and the healthy aphid 500 of picking, isolates plant.Obtain 1000 times of liquid with after pulvis 10g of the present invention, liquor 10g thin up, within every two weeks, use the present invention once.Experimental result: after one month, crape myrtle children browse haunts without ant, and Aphed population obviously reduces, and quantity is 26; Observe crape myrtle growing state without exception; Aphid lethality is 94.8%.
Embodiment 4
On July 21st, 2014 chooses vegetable aphid worm as experiment material, and the healthy aphid 500 of picking, isolates plant.Obtain 1000 times of liquid with after pulvis 10g of the present invention, liquor 10g thin up, within every two weeks, use the present invention once.Experimental result: after one month, vegetables children browse haunts without ant, and Aphed population obviously reduces, and quantity is 24; Observe vegetable growth situation without exception; Aphid lethality is 95.2%.
Claims (2)
1. a biopesticide compound method for killing aphids, is characterized in that following steps:
(1) the camphor tree branches and leaves in period short of rain in autumn are got, put into mortar after shredding to grind, steam distilled method is used after abundant grinding, the steam of camphor and camphor oil that has obtained is entered the dish type cooler of many rings again through wireway, the mixture of camphor and camphor oil and water can be obtained, by leaving standstill rear layering, remove camphor and the camphor oil mixture of gained after moisture, entered multiple method of steaming again and collected distillate under the condition of 155-200 DEG C, the crystalline solid of separating out after the distillate now collected cooling was crystallized camphor;
(2) get ash fully to mix with water, mixed weight ratio is 1: 1, and ash, after entering circadian immersion, filters gained supernatant and is leachate;
(3) white muscardine fungi powder, green muscardine fungus powder and yeast powder is taken by the weight of each component, mix adding pulvis without the white muscardine fungi powder activated, green muscardine fungus powder and yeast powder with biopesticide, obtain Mixed Microbes pulvis I, guarantee under the dry condition, avoid bacterial classification at medication front activating;
(4) take a small amount of glucose as above-mentioned actication of culture basic material by the weight of each component, glucose is made pulvis;
(5) stir after taking crystallized camphor, ash leachate, Mixed Microbes pulvis I, the mixing of glucose pulvis by each component.
2. the biopesticide preparation method of a kind of killing aphids according to claim 1, it is characterized in that: each composition weight number of biopesticide is camphor 3-4 part, ash leachate 10-15 part, white muscardine fungi powder 0.2-0.3 part, green muscardine fungus powder 0.3-0.4 part, yeast powder 0.05-0.08 part, glucose pulvis 5-8 part.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106258594A (en) * | 2016-08-25 | 2017-01-04 | 周宁县绿绿山农林综合开发有限公司 | Taxus mairei culture medium and breeding method |
| CN106818911A (en) * | 2016-12-31 | 2017-06-13 | 新昌县迪斯曼科技有限公司 | Public nuisance-free agricultural chemicals containing microorganism of green and preparation method thereof |
| CN107156149A (en) * | 2017-05-24 | 2017-09-15 | 宁夏天瑞产业集团现代农业有限公司 | A kind of high-efficiency plant protective agent and preparation method thereof |
| CN107494626A (en) * | 2017-07-24 | 2017-12-22 | 张剑秋 | One kind is used for non-harmful botanical pesticide of afforestation and preparation method thereof |
| CN108713469A (en) * | 2018-04-23 | 2018-10-30 | 临武县农业综合服务中心 | Linwu glycan cultural method |
| CN109042189A (en) * | 2018-07-27 | 2018-12-21 | 上海环垦生态科技股份有限公司 | A kind of implantation methods reducing Paris polyphylla disease |
| CN109730093A (en) * | 2018-06-04 | 2019-05-10 | 北京东方昊天生物技术研究所有限公司 | A kind of aphid drives microbial inoculum and its preparation method and application |
| CN110896964A (en) * | 2019-12-19 | 2020-03-24 | 河南农贝得农业科技有限公司 | Seed dressing agent without pesticide residue and preparation method thereof |
| CN112514917A (en) * | 2020-12-03 | 2021-03-19 | 黑龙江沪生农业发展有限公司 | Composite microbial pesticide and preparation method thereof |
| CN113712029A (en) * | 2021-09-06 | 2021-11-30 | 四川宸煜林业开发有限责任公司 | Pinene-containing plant protection additive and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258594A (en) * | 2016-08-25 | 2017-01-04 | 周宁县绿绿山农林综合开发有限公司 | Taxus mairei culture medium and breeding method |
| CN106818911A (en) * | 2016-12-31 | 2017-06-13 | 新昌县迪斯曼科技有限公司 | Public nuisance-free agricultural chemicals containing microorganism of green and preparation method thereof |
| CN107156149A (en) * | 2017-05-24 | 2017-09-15 | 宁夏天瑞产业集团现代农业有限公司 | A kind of high-efficiency plant protective agent and preparation method thereof |
| CN107494626A (en) * | 2017-07-24 | 2017-12-22 | 张剑秋 | One kind is used for non-harmful botanical pesticide of afforestation and preparation method thereof |
| CN108713469A (en) * | 2018-04-23 | 2018-10-30 | 临武县农业综合服务中心 | Linwu glycan cultural method |
| CN109730093A (en) * | 2018-06-04 | 2019-05-10 | 北京东方昊天生物技术研究所有限公司 | A kind of aphid drives microbial inoculum and its preparation method and application |
| CN109730093B (en) * | 2018-06-04 | 2021-07-20 | 北京东方昊天生物技术研究所有限公司 | Aphid repellent agent and preparation method and application thereof |
| CN109042189A (en) * | 2018-07-27 | 2018-12-21 | 上海环垦生态科技股份有限公司 | A kind of implantation methods reducing Paris polyphylla disease |
| CN110896964A (en) * | 2019-12-19 | 2020-03-24 | 河南农贝得农业科技有限公司 | Seed dressing agent without pesticide residue and preparation method thereof |
| CN112514917A (en) * | 2020-12-03 | 2021-03-19 | 黑龙江沪生农业发展有限公司 | Composite microbial pesticide and preparation method thereof |
| CN113712029A (en) * | 2021-09-06 | 2021-11-30 | 四川宸煜林业开发有限责任公司 | Pinene-containing plant protection additive and preparation method thereof |
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