CN101438714A - Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof - Google Patents
Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof Download PDFInfo
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- CN101438714A CN101438714A CNA2008102200026A CN200810220002A CN101438714A CN 101438714 A CN101438714 A CN 101438714A CN A2008102200026 A CNA2008102200026 A CN A2008102200026A CN 200810220002 A CN200810220002 A CN 200810220002A CN 101438714 A CN101438714 A CN 101438714A
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- destruxins
- beauveria bassiana
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Abstract
The invention discloses a pesticide compounding beauveria bassiana fungus and destruxin. The pesticide is prepared by mixing the beauveria bassiana fungus, the destruxin and solvent, the concentration of live conidia of the beauveria bassiana fungus after mixing ranges from 10<7> to 10<11> conidia per liter, and the pesticidal effect is better when the concentration of the destruxin ranges from 10 mg/L to 100 mg/L. The preparation method for the pesticide comprises the following steps: preparing conidial suspension of the beauveria bassiana fungus, preparing destruxin stock solution, mixing the conidial suspension and the destruxin stock solution, and the like. The invention selects the beauveria bassiana fungus to be used with the destruxin in a mixing way for synergic action, and the pesticide has the completely new function mechanism different form the prior pesticide, has an excellent pesticidal effect particularly in poisoning and killing bemisia tabaci gennadius with serious harm, and meanwhile is environment-friendly.
Description
Technical field
The invention belongs to biological pesticide technical field, relate to a kind of beauveria bassiana fungi and destruxins mixed insecticide and preparation method thereof.
Background technology
The preventing and controlling of important agriculture and forestry insects such as Lepidoptera, Semiptera, Homoptera, coleoptera are extremely important.For example Bemisia tabaci Bemisia tabaci (Gennadius) belongs to Homoptera (Hopoptera) Aleyrodidae (Aleyrodidae), is a kind of global important pests.Along with the appearance of Type B Bemisia tabaci, Bemisia tabaci is serious day by day to the harm of field crop, vegetables and flowers etc. after the seventies in 20 century, and especially in recent years, Bemisia tabaci was broken out greatly in succession in all over the world.Bemisia tabaci was broken out in China different regions in succession since the initial stage nineties, crops such as the cucumber of especially seriously causing harm, tomato, eggplant, muskmelon and custard squash, and percent of loss can reach more than 70% when serious.Bemisia tabaci has that the bodily form is small, and population quantity is big, and host range is wide, and generation overlap is serious, easy characteristics such as diffusion, and the target locus gene of agricultural chemicals is easy to produce sudden change and obtains accumulating in the polypide, and heredity continues rapidly.Therefore, the pesticide resistance problem of Bemisia tabaci is very serious, main type insecticide such as organochlorine, organic phosphor, pyrethroid, carbamate, nicotinoids, insect growth regulator, IGR has been produced pesticide resistance at present.Biological control has become the technology of the essential development of control Bemisia tabaci.
Beauveria bassiana (Beauveris bassiana) is a kind of important insect pathogenic fungus, insects such as Homoptera, Lepidoptera, diptera, coleoptera, Hymenoptera there is infection ability, is mainly used in important agriculture and forestry insects such as control Lepidoptera, Semiptera, Homoptera, coleopteran pest.Beauveria bassiana is the same with other fungus insecticide, have free from environmental pollution, do not develop immunity to drugs and advantage such as lasting effectiveness, yet, use beauveria bassiana to prevent and treat insects such as Bemisia tabaci separately, its effect instability, be subjected to environmental influence very big, and it is slow to take effect.Therefore, the mix preparation of development fungi is a good method that overcomes its inherent defect.
Destruxins (destruxin) is an insect pathogenic fungus---a kind of cyclic depsipeptides antibiotic that green muscardine fungus (Metarhiziumanisopliae var.anisopliae) secretes to the host insect haemocoele in infection processs, destruxins has significant insecticidal effect, and has the brand-new mechanism of action that is different from existing insecticide, mainly act on the hemolymph immune system of insect, make it to lose the immune defense ability.Destruxins is easily degraded, noresidue, and toxicity is low, and the development and use destruxins has important economic implications.Yet when destruxins used separately, virulence was not high enough, and control efficiency is unsatisfactory.
Simultaneously, the long-term use with a kind of insecticide causes the pest resistance to insecticide development easily, and this has also constantly proposed new demand for development to mixed insecticide, and continually developing research agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method.
In sum, the applicant intends utilizing the synergistic effect of destruxins to entomogenous fungi, exploitation beauveria bassiana and destruxins mixed insecticide, and especially at the insecticide of Bemisia tabaci, the temporarily no at present technology of correlation technique is reported.
Summary of the invention
An object of the present invention is to overcome the deficiencies in the prior art, a kind of beauveria bassiana fungi and destruxins mixed insecticide are provided.
Another object of the present invention provides described method for producing insecticide.
Purpose of the present invention realizes by following technical proposals:
A kind of beauveria bassiana fungi and destruxins mixed insecticide are provided, adopt beauveria bassiana fungi and destruxins to be mixed and form, described insecticide contains beauveria bassiana fungi conidium 10 alive
7～11Individual/L, contain destruxins 10～100mg/L.
The assistant agent of using that is mixed is the conventional solvent that uses, for example Tween 80, acetone or ethyl acetate equal solvent.
Described destruxins can be any homologue in the destruxins, or they are with the mixture of any ratio.
The present invention provides the preparation method of described beauveria bassiana fungi and destruxins mixed insecticide simultaneously, may further comprise the steps:
(1) preparation beauveria bassiana fungus conidium suspension;
(2) preparation destruxins stock solution;
(3) conidial suspension is mixed with the destruxins stock solution.
Conidial concentration of living in the described conidial suspension of step (1) is 10
10～12Individual/L.The beauveria bassiana fungus conidium can be prepared with 0.01～0.05% Tween 80 solution dilution.
The concentration of destruxins is greater than 100000mg/L in the described destruxins stock solution of step (2), and it is formulated with solvent acetone or acetic acid ethyl dissolution to get destruxins.
The described beauveria bassiana fungus conidium of step (3) suspension is that the described beauveria bassiana fungus conidium of above-mentioned steps (1) suspension is mixed by a certain percentage with the described destruxins stock solution of step (2) with mixing of destruxins stock solution, as long as make ultimate density remain beauveria bassiana fungi conidium 10 alive
7～11Individual/L, destruxins concentration is that 10～100mg/L gets final product.
Insecticide use of the present invention is that the mixed liquor that above-mentioned steps (3) prepares is sprayed on target crop, carries out the insect field control.
The invention has the beneficial effects as follows:
The invention provides a kind of new fungal organism insecticide, carry out biological control at the pesticide resistance problem of Bemisia tabaci.The present invention is by long-term a large amount of experimental study, select beauveria bassiana fungi and destruxins to use the pesticide resistance problem that synergy solves Bemisia tabaci with, by a large amount of experimental studies, insecticide after determining to be mixed guarantees preferable insecticidal effect, beauveria bassiana fungus conidium wherein and destruxins concentration.Described insecticide has the brand-new mechanism of action that is different from existing insecticide, and insecticidal effect is remarkable, and is especially remarkable at the serious Bemisia tabaci toxic action of harm, has eco-friendly characteristics simultaneously.Insecticide preparation method of the present invention is very simple, is easy to promote.
Embodiment
Always further describe the present invention below in conjunction with concrete enforcement.
Embodiment 1: the preparation of Synergistic insecticidal of the present invention
(1) get beauveria bassiana bacterium conidium, being mixed with concentration with 0.01～0.05% Tween 80 solution is 10
10～12The spore suspension of individual conidium/L alive;
(2) get destruxins, be mixed with the stock solution of effective ingredient concentration greater than 100000mg/L with acetone or ethyl acetate equal solvent;
(3) the described conidial suspension of above-mentioned steps (1) is mixed by a certain percentage with the described destruxins stock solution of step (2), make ultimate density remain conidium 10 alive
9～11Individual/L, destruxins 10～100mg/L.
Embodiment 2 Synergistic insecticidals of the present invention are to the Toxicity Determination experiment of agriculture and forestry insect
Present embodiment is main experimental subjects with Bemisia tabaci (Bemisia tabaci), when carrying out Toxicity Determination, make test plant with Japan (Hibiscus rosa-sinensisL), its potted plant seedling is educated in dependent insect cage, Bemisia tabaci adults is inserted dependent insect cage, treat after it was laid eggs one day it all to be driven out of, keep 26 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on Japan's blade grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, repeat 4 times, 4 days effect behind the investigation medicine, the calculation correction lethality is asked the regression equation of drug concentration logarithm-lethality probability value, and the comptibility test of the side of card, obtain LC then
50, calculate synergic ratio (SR) according to following formula, whether have synergistic effect: SR≤0.5, antagonism according to SR value criterion intermixture; SR=0.5～1.5, summation action; SR 〉=1.5 synergistic effects.
LC
50(theory)=(a+b)/(a/LC
50A+ b/LC
50B)
Synergic ratio (SR)=LC
50Theoretical value/LC
50Real value
A, B represent two kinds of medicament components respectively, and a, b are the ratio of A, B two components content in mixture.
Because experimental technique belongs to this area conventional method, experimental result is not given unnecessary details one by one, and part of test results sees Table 1, and beauveria bassiana conidium and destruxins mixed insecticide have remarkable insecticidal synergistic effect.
Table 1 beauveria bassiana conidium and destruxins are to the indoor virulence experimental result of Bemisia tabaci
CS: conidium, its LC
50Value unit is 10
6Individual/L; DA: destruxins A, its LC
50Value unit is mg/L.
Embodiment 3 insecticides of the present invention are to the field control effectiveness test of Bemisia tabaci
Tested in May, 2008 and on the greenhouse cultivation tomato, carry out, 22～30 ℃ of temperature, relative moisture 81～97%, sub-district area 30m
2, randomised block design repeats 4 times, and at Bemisia tabaci nymph peak period in 2 age spray medicine, every investigation in 2 days once, every sub-district is fixed and is checked 10 point (every about 10cm after the dispenser
2Leaf area), each point is no less than 100 nymphs, writes down the dead borer population of total borer population and (disease), the calculation correction lethality is made variance analysis and multiple range test.Result of the test sees that as table 2 control efficiency of Synergistic insecticidal of the present invention obviously is better than beauveria bassiana and the agent of destruxins list.
Several medicaments of table 2 contrast Bemisia tabaci (Bemisia tabaci) control efficiency
Claims (7)
1, a kind of beauveria bassiana fungi and destruxins mixed insecticide is characterized in that it being to adopt beauveria bassiana fungi, destruxins and solvent to be mixed to form, and described beauveria bassiana fungi conidial concentration alive is 10
7～11Individual/L, the concentration of described destruxins is 10～100mg/L.
2, the preparation method of described beauveria bassiana fungi of a kind of claim 1 and destruxins mixed insecticide is characterized in that may further comprise the steps:
(1) preparation beauveria bassiana bacterium conidial suspension;
(2) preparation destruxins stock solution;
(3) conidial suspension is mixed with the destruxins stock solution.
3, according to the preparation method of described beauveria bassiana fungi of claim 2 and destruxins mixed insecticide, conidial concentration that it is characterized in that living in the described conidial suspension of step (1) is 10
10～12Individual/L.
4, according to the preparation method of claim 2 or 3 described beauveria bassiana fungies and destruxins mixed insecticide, the preparation that it is characterized in that the described suspension of step (1) is that the beauveria bassiana fungus conidium is prepared with 0.01～0.05% Tween 80 solution dilution.
5, according to the preparation method of described beauveria bassiana fungi of claim 2 and destruxins mixed insecticide, the concentration that it is characterized in that destruxins in the described destruxins stock solution of step (2) is greater than 100000mg/L.
6, according to the preparation method of claim 2 or 5 described beauveria bassiana fungies and destruxins mixed insecticide, the preparation that it is characterized in that the described destruxins stock solution of step (2) is to get destruxins to dissolve formulated with acetone or ethyl acetate solvent.
7,, it is characterized in that the mixed proportion of described conidial suspension of step (3) and destruxins stock solution is 10 according to the conidium ultimate density of living according to the preparation method of described beauveria bassiana fungi of claim 2 and destruxins mixed insecticide
9～11Individual/L, destruxins ultimate density is that 10～100mg/L determines.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102200026A CN101438714B (en) | 2008-12-15 | 2008-12-15 | Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102200026A CN101438714B (en) | 2008-12-15 | 2008-12-15 | Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101438714A true CN101438714A (en) | 2009-05-27 |
| CN101438714B CN101438714B (en) | 2011-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102200026A Expired - Fee Related CN101438714B (en) | 2008-12-15 | 2008-12-15 | Compounding pesticide containing Beauveria bassiana and destruxins of Metarrhizium anisopliae and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532286A (en) * | 2011-10-20 | 2012-07-04 | 中国科学院微生物研究所 | Beauvericin synthesis relative protein system and coding gene cluster and application thereof |
| CN104351260A (en) * | 2014-10-14 | 2015-02-18 | 深圳文科园林股份有限公司 | Biological pesticide for killing aphids and preparation method for biological pesticide |
| CN106489995A (en) * | 2016-09-30 | 2017-03-15 | 河北素染地农业开发有限公司 | A kind of biopesticide synergist and preparation method thereof |
| CN107156149A (en) * | 2017-05-24 | 2017-09-15 | 宁夏天瑞产业集团现代农业有限公司 | A kind of high-efficiency plant protective agent and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100477920C (en) * | 2006-12-26 | 2009-04-15 | 华南农业大学 | Compounding insecticide containing destruxin and abamectin |
-
2008
- 2008-12-15 CN CN2008102200026A patent/CN101438714B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532286A (en) * | 2011-10-20 | 2012-07-04 | 中国科学院微生物研究所 | Beauvericin synthesis relative protein system and coding gene cluster and application thereof |
| CN102532286B (en) * | 2011-10-20 | 2014-05-28 | 中国科学院微生物研究所 | Beauvericin synthesis relative protein system and coding gene cluster and application thereof |
| CN104351260A (en) * | 2014-10-14 | 2015-02-18 | 深圳文科园林股份有限公司 | Biological pesticide for killing aphids and preparation method for biological pesticide |
| CN106489995A (en) * | 2016-09-30 | 2017-03-15 | 河北素染地农业开发有限公司 | A kind of biopesticide synergist and preparation method thereof |
| CN107156149A (en) * | 2017-05-24 | 2017-09-15 | 宁夏天瑞产业集团现代农业有限公司 | A kind of high-efficiency plant protective agent and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN101438714B (en) | 2011-08-17 |
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