CN109730093A - A kind of aphid drives microbial inoculum and its preparation method and application - Google Patents
A kind of aphid drives microbial inoculum and its preparation method and application Download PDFInfo
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- CN109730093A CN109730093A CN201811629756.7A CN201811629756A CN109730093A CN 109730093 A CN109730093 A CN 109730093A CN 201811629756 A CN201811629756 A CN 201811629756A CN 109730093 A CN109730093 A CN 109730093A
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- aphid
- cgmcc
- rhodococcus
- microbial inoculum
- saccharomyces
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Abstract
The present invention relates to field of biological pesticide, and in particular to a kind of aphid drives microbial inoculum and its preparation method and application.It includes saccharomycete (Saccharomyces) CGMCC No.3278 and Rhodococcus sp (Rhodococcus) CGMCC No.3276 that the aphid, which drives microbial inoculum,.Aphid of the invention, which drives microbial inoculum, can be significantly reduced aphid disease incidence, while can also promote crop yield.
Description
Technical field
The present invention relates to field of biological pesticide, and in particular to a kind of aphid drives microbial inoculum and its preparation method and application.
Background technique
Aphid is important pests on the plants such as wheat and fruits and vegetables, since aphid occurs to be exceedingly fast with reproduction speed, not only directly
Feeding plant juice, while various plants virus is propagated, heavy losses are caused to wheat and fruits and vegetables Isoquant and quality.
At present in fact prevention and control of aphids mainly use chemical prevention because aphid reproduction speed is exceedingly fast, other methods by
In various reasons, it is difficult to complete efficiently quick disease and pest control.Its conventional application method of chemical prevention be aphid occur the phase into
Row complete stool is spraying, and side effect is not only direct killing natural enemy, pollution environment, and is depending on chemical pesticide unduly and using not
Cause to remain under reasonable status and exceeded seriously affects food safety.Therefore, in order to reduce pesticide initiation many serious adverses
It influences, there is an urgent need to develop non-environmental pollution control technologies for the prevention and treatment of the plant insects such as wheat and fruits and vegetables.
Summary of the invention
The object of the present invention is to provide a kind of aphids to drive microbial inoculum.
Another object of the present invention is to provide a kind of method for reducing aphid incidence.
Aphid according to the present invention drives microbial inoculum, including saccharomycete (Saccharomyces) CGMCC No.3278) He Hongqiu
Bacterium (Rhodococcus) CGMCC No.3276.
Aphid according to the present invention drives microbial inoculum, wherein saccharomycete (Saccharomyces) CGMCC No.3278) and red ball
The volume ratio of bacterium (Rhodococcus) CGMCC No.3276 is 2:1.
The present invention also provides the methods for preparing above-mentioned aphid drive microbial inoculum, and the method includes expanding culture yeasts bacterium
(Saccharomyces) CGMCC No.3278) and the step of Rhodococcus sp (Rhodococcus) CGMCC No.3276, wherein expand
The formula of big culture medium are as follows: sucrose 100g, peptone 7g, yeast extract 7g, KH2PO41g, MgSO40.5, distilled water 1L, pH 4.0
~6.5.
The present invention also provides a kind of methods for reducing aphid incidence, and the method includes spraying saccharomycete to plant complete stool
(Saccharomyces) CGMCC No.3278) and Rhodococcus sp (Rhodococcus) CGMCC No.3276 mixing liquid step
Suddenly.
The method according to the present invention for reducing aphid incidence, wherein saccharomycete (Saccharomyces)
It CGMCCNo.3278 is) 2:1 with the volume ratio of Rhodococcus sp (Rhodococcus) CGMCC No.3276.
The present invention also provides above-mentioned aphids to drive microbial inoculum for reducing the application of plant aphids disease incidence, wherein the plant
Object can be crops, vegetables and fruit crop.
The present invention also provides above-mentioned aphids to drive the application that microbial inoculum is used to improve crop yield, and the crops are jade
Rice, wheat, soybean and/or rice.
Aphid according to the present invention, which drives microbial inoculum, can be significantly reduced aphid disease incidence, while can also promote crop yield,
Welding does not form, and applies at low cost, and anthelminthic effect is significant.
Detailed description of the invention
The growing state that aphid of the invention drives wheat after microbial inoculum is sprayed in Fig. 1 display.
Specific embodiment
Embodiment 1 prepares aphid and drives microbial inoculum
Saccharomycete (Saccharomyces) CGMCC No.3278 and Rhodococcus sp (Rhodococcus) CGMCC No.3276
Volume ratio be 2:1.The greasy clever bacteria agent of drive is produced with culture medium production expansion culture is expanded, expands the formula of culture medium
Are as follows: sucrose 100g, peptone 7g, yeast extract 7g, KH2PO41g, MgSO40.5, distilled water 1L, pH are 4.0~6.5.
And will be enlarged by culture and carry out 100 times of dilutions, it is transferred in sprayer after mixing evenly, direct complete stool is spraying.Root
Determine that the number of sprinkling, such as wheat generally spray 2-3 times according to plant growth characteristics.
The control for not applying any chemistry and biological pesticide is set, and setting only applies the control of chemical pesticide, applies CGMCC
The test group of No.3278 applies the test group of CGMCC No.3276, applies saccharomycete (Saccharomyces) CGMCC
The test group of No.3278 and Rhodococcus sp (Rhodococcus) CGMCC No.3276.
Test result shows that aphid of the invention drives microbial inoculum and reduces aphid disease incidence about 90% or more, increases production 10-
20%.Pesticide reduces aphid disease incidence about 60-70%.
Test result shows to apply saccharomycete (Saccharomyces) CGMCC No.3278 and Rhodococcus sp
(Rhodococcus) test group of CGMCC No.3276 has synergistic technical effect in terms of reducing aphid incidence.
10 days-May 20 April in 2017, aphid of the invention drive microbial inoculum and dilute 100 times, start complete stool and are sprayed, by spraying altogether
Meter 2 times, once produces 300 jin per acre by 1300 jin of per mu yield.The wheat other than 8 mu of experimental plots uses cypermethrin pesticide 3 at the same time
It is secondary, per acre 500 milliliters every time, direct complete stool sprinkling, 1000 jin of per mu yield.
The 5-8 month in 2017, the Zhao County the Yu Hebei pear place of production have been done not to pear jacketing experiment, but in aphid arriving value
Aphid is driven into microbial inoculum in advance before and dilutes 100 times, starts complete stool and is sprayed, amount to 4 times by spraying, 2800 jin of per mu yield, once produce 840 per acre
Jin.1960 jin of the per mu yield of bagging pear at the same time.
Claims (9)
1. a kind of aphid drives microbial inoculum, which is characterized in that it includes saccharomycete (Saccharomyces) CGMCC that the aphid, which drives microbial inoculum,
No.3278 and Rhodococcus sp (Rhodococcus) CGMCC No.3276.
2. aphid according to claim 1 drives microbial inoculum, which is characterized in that saccharomycete (Saccharomyces) CGMCC
The volume ratio of No.3278 and Rhodococcus sp (Rhodococcus) CGMCC No.3276 is 2:1.
3. a kind of method for preparing aphid and driving microbial inoculum, which is characterized in that the method includes expanding culture yeasts bacterium
(Saccharomyces) the step of CGMCC No.3278 and Rhodococcus sp (Rhodococcus) CGMCC No.3276, wherein expand
The formula of big culture medium are as follows: sucrose 100g, peptone 7g, yeast extract 7g, KH2PO41g, MgSO40.5, distilled water 1L, pH 4.0
~6.5.
4. a kind of method for reducing aphid incidence, which is characterized in that the method includes spraying saccharomycete to plant complete stool
(Saccharomyces) CGMCC No.3278) and Rhodococcus sp (Rhodococcus) CGMCC No.3276 mixing liquid step
Suddenly.
5. the method according to claim 4 for reducing aphid incidence, which is characterized in that saccharomycete
(Saccharomyces) CGMCC No.3278) with the volume ratio 2:1 of Rhodococcus sp (Rhodococcus) CGMCC No.3276.
6. aphid described in claim 1 drives microbial inoculum for reducing the application of plant aphids disease incidence.
7. application according to claim 6, which is characterized in that the plant is crops, vegetables and/or fruit crop.
8. benefit requires 1 aphid to drive the application that microbial inoculum is used to improve crop yield.
9. application according to claim 8, which is characterized in that the crops are corn, wheat, soybean and/or rice.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018105625604 | 2018-06-04 | ||
| CN201810562560 | 2018-06-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109730093A true CN109730093A (en) | 2019-05-10 |
| CN109730093B CN109730093B (en) | 2021-07-20 |
Family
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| CN201811629756.7A Active CN109730093B (en) | 2018-06-04 | 2018-12-29 | Aphid repellent agent and preparation method and application thereof |
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| CN109730093B (en) | 2021-07-20 |
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