CN103484404A - Efficient repairing complex inoculant and application thereof - Google Patents
Efficient repairing complex inoculant and application thereof Download PDFInfo
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- CN103484404A CN103484404A CN201310430142.7A CN201310430142A CN103484404A CN 103484404 A CN103484404 A CN 103484404A CN 201310430142 A CN201310430142 A CN 201310430142A CN 103484404 A CN103484404 A CN 103484404A
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Abstract
The invention relates to the field of biology and in particular to an efficient repairing complex inoculant and application thereof. The complex inoculant provided by the invention comprises strains of rhodococcus, pseudomonas, saccharomyces and micrococcus luteus, where the preservation number of the rhodococcus is CGMCC No.3276; the preservation number of the pseudomonas is CGMCC No.3277; the preservation number of the saccharomyces is CGMCC No.3278; the preservation number of the micrococcus luteus is CGMCC No.3903. The complex inoculant has remarkable effects of water ecosystem restoration, water bloom prevention and deodorization, in addition, the removal rate of the complex inoculant to all organic matters reach more than 70 percent, and national policies of circular economy and energy conservation and emission reduction are met better. By using the complex inoculant, a large amount of running cost for various water areas can be saved, and recycling treatment to wastewater is achieved.
Description
Technical field
The present invention relates to the decomposing biological field, be specifically related to efficiently repair composite fungus agent and application thereof.
Background technology
The water body biologic chain is in the formation and development process, and consist of each period its specific Ecological Characteristics and fixing structure, thereby shows the ecological processes such as certain material cycle and flow of energy.When extraneous interference is weak, the water body biologic chain can show certain capacity of self-regulation, to maintain its intrinsic structure and function; When disturbing over its capacity of self-regulation, the biologic chain structure and function just there will be larger variation, the chain rupture link even occurs.The water body biologic chain that chain rupture occurs, its structure and function generally shows as that species diversity descends, the population regulating power is lost, the system primary productivity descends, the material cycle process is obstructed, structure of community is simplified and species composition proportional imbalance etc.
Several large difficult point that polluted-water reparation, purification high efficiency composition microbial inoculum technology exist: how to screen, separate, select the startup excellent, that microbial inoculum and ecosystem reparation are cultivated in combination, the input etc. of debugging, select a good opportunity.Science is used the efficient composite fungus agent of repairing, repair the combination of unit and mesophytia structure by biologic chain and pollute the application in controlling and administering at river water body, can effectively solve the with serious pollution environmental problem of eutrophication water, repair the aquatic ecosystem function.
It is nothing but Physical, chemical method and biological process that the improvement method of body eutrophication sums up.As cut dirt, water transfer, desilting etc., need the large-scale structures of building in Physical and chemical method, expense is high, although chemical method flocculation successful, chemical sludge output is large, and certain toxicity is arranged, and can cause secondary pollution.Due to the continuous release of Sediments nutritive substance, the increase of the various dried wet depositions in ground, Physical and chemical method can not fundamentally solve wawter bloom and the black smelly problem of water body.Therefore, along with the raising of environmental requirement and the development of Environmental Biotechnology, biological restoration is administered skill as a kind of low investment, high benefit, the convenient, flexible water pollution of operation.At present, the applied research of bacteria agent recovery technique has started to walk to develop and has been applied in the middle of production reality, some achievements in research have been obtained, but be mostly external import microbial inoculum, the reparation that how to take full advantage of China's autochthons and develop, the applied research of purification microbial inoculum technology on eutrophication water, report seldom.
There is following defect in existing recovery technique:
1, single artificial aeration's reoxygenation technology, although can effectively improve water quality in a short time, have temporary, unstable and the shortcoming such as cure the symptoms, not the disease, and denitrogenation dephosphorizing efficiency is lower.
2, single microorganism recovery technique.
By compound bio recovery technique of the present invention, not only solved wawter bloom and the black smelly problem in river lake, and greatly improved local ecotope.
Summary of the invention
In order to address the above problem, the present invention's research, utilize and find superior microorganism, progressively recovers the biologic chain ruptured, take full advantage of the biological self cleaning function of the Nature and the microbial technique in scientific research, allow the local ecosystem progressively become stable by current fragility.
Therefore, the purpose of this invention is to provide efficient reparation composite fungus agent.
A further object of the present invention is to provide the application of above-mentioned efficient reparation composite fungus agent.
The efficient composite fungus agent of repairing be take the aerobic-anaerobic microbe flora as main, not high to the demand of oxygen, still can breed rapidly, and become the dominant microflora in water body when water surrounding bottom dissolved oxygen is zero or very low.In this process, the variation of matter has occurred in microorganisms in water group, by anaerobism to facultative, aerobic transition.Amphimicrobe and aerobic flora are by the organic pollutant fast degradation in water body, and water quality progressively improves.Dissolved oxygen content in water body progressively improves, and has effectively suppressed activity and the fecundity thereof of anerobe.Water body apparent by black gradually to tawny, faint yellow, until to the transition of normal water body colour.It is zero or the aerobic bacteria slow difficult point of rising in value when extremely low that composite fungus agent has been captured Dissolved Oxygen in Water, has successfully realized quick startup and the steady running of biologic chain recovery technique in the aquatic ecosystem recovery project.
Reparation composite fungus agent according to the present invention comprises: Rhod (Rhodococcus) (deposit number CGMCC No.3276, open in CN101748091), Rhodopseudomonas (Pseudomonas) (open in deposit number CGMCC No.3277CN101748091), Saccharomycodes (Saccharomyces) (open in deposit number CGMCC No.3278CN101748091) and micrococcus sp (Micrococcus luteus) (deposit number CGMCC No.Open in 3903CN102329744A), the ratio of Rhod (Rhodococcus) Rhodopseudomonas (Pseudomonas) Saccharomycodes (Saccharomyces) micrococcus sp (Micrococcus luteus) four strain deodorant predominant bacterias in composite fungus agent is respectively 3:3:2:2.After they remove COD and are respectively 88.3%, 83.1%, 77.5%, 76.2% 4 strain bacterium combination, treatment effect is 91.6%; Remove that TN is respectively 72.4%, 65.2%, 55.3%, after 53.8%. tetra-strain bacterium combinations, treatment effect is 74.8%, as can be seen here, composite fungus agent has produced the effect of synergy aspect denitrogenating.
The present invention uses above-mentioned composite fungus agent to process black smelly river, the composite fungus agent substratum: yeast extract 10g/L, and extractum carnis 5g peptone 10g/L,, MgSO
47H
2o20g/L, KCL2g/L, NaCL5g/L, pH7.0; 25-35 ℃.
Composite fungus agent at the scene during enlarged culturing, is adopted to above-mentioned substratum, by the needed amount in scene, carry out enlarged culturing.The needed composite fungus agent in scene is added in combination biological restoration treatment system.The ratio that adds is relevant with COD concentration and the sewage quantity of sewage.Below divide three kinds of situations a) when COD concentration is less than 100mg/L, by the 1/100000(100,000 of system/) volumetric ratio added, and in the 100000m3 volume, adds 1m
3efficient reparation composite fungus agent.When b) COD concentration is less than 1000mg/L and is greater than 100mg/L, by the 1/10000-5/10000(ten thousand of system/5/10,000) volumetric ratio added, i.e. 10000m
3add 1m in volume
3-5m
3efficient reparation composite fungus agent.When c) COD concentration is greater than 1000mg/L, by 1/1000 volumetric ratio of system, added, in the 1000m3 volume, added 1m
3efficient reparation composite fungus agent.Simultaneously, regularly add the composite fungus agent substratum, make the growth that microbial inoculum is good at the biosystem energy and form microbial film, the unloading phase of having completed.
The unloading phase complete after, enter the domestication stage.That is: reduce gradually the amount that adds the composite fungus agent substratum and increase gradually black smelly river, until the dosage of composite fungus agent substratum is 0%, the amount of black smelly river water is increased to 100%.
After starting and having tamed, just enter whole biosystem and equipment debugging, operation, finally make effluent quality reach the landscape water water quality standard.
Technical scheme of the present invention has the following advantages:
(1) original position multi-point sampling method filters out many strains microorganism, the bacterium liquid poor stability that the single-point sampling is cultivated, the difficult point easily be hit have been overcome, to whole black and odorous river administer research play most important effect, be the core of aquatic ecosystem bioremediation technology.
(2), by a large amount of single factor and orthogonal experiment, realized that selecting of bacterial strain is excellent and grasped the growth conditions of efficient reparation composite fungus agent the best.
(3) broken through by the single creature recovery technique, proposed directly to render in contaminated water body; Or with build the common implementings such as artificial swamp, artificial aeration's reoxygenation, make microbial strains can adhere to for a long time procreation, finally recover the ecology of contaminated river course, water body in lake.And this technology is applied to the improvement of the control of actual wawter bloom and black and odorous river.
(4) wawter bloom of the present invention control deodorizing effect significantly outside, the clearances such as organism are all reached more than 70% simultaneously.Always know, compare with other treatment technologies, more meet the national policy of " recycling economy ", " energy-saving and emission-reduction ".It can be a large amount of running cost of waters, various places saving, realizes the recycling treatment of waste water.
The accompanying drawing explanation
Fig. 1 shows efficient reparation composite fungus agent preparation and the aquatic ecosystem water body is repaired, the cleaning project applicating flow chart.
Embodiment
Embodiment 1
(1) enrichment medium:
1. yeast: glucose 100g, peptone 7g, yeast extract paste 7g, KH2PO41g, MgSO40.5, distilled water 1L, pH is 4.0～6.5;
2. photosynthetic bacterium: NH4Cl0.1g, NaHCO
3(0.1g 5% aqueous solution, filtration sterilization is got 2ml and added), K
2hPO
40.02g, sodium acetate 0.3g, MgSO
47H
2o0.02g, NaCl0.2g, somatomedin 1ml, trace element solution 1ml, distilled water 97ml, pH7.0.
3. heterotroph nitrifier: beef peptone 15g/L, NaCL5g/L, pH7.0:25-35 ℃.
Isolation medium:
1. yeast: potato 200g(peeling, dig eye, clean section, claim 200g to put into the 1000ml tap water 30min that boils that simmers in water, double gauze filters, and filtrate is diluted with water to 1000ml), glucose 20g, lactic acid 5ml, agar 20g, distilled water 1000ml, pH is 4.0～6.5;
2. photosynthetic bacterium: NH4Cl0.1g, NaHCO
3(0.1g 5% aqueous solution, filtration sterilization is got 2ml and added), K
2hPO
4, 0.02g, sodium acetate 0.3g, MgSO
47H
2o0.02g, NaCl0.2g, somatomedin 1ml, trace element solution 1ml, distilled water 97ml, agar 20g, pH7.0.
3. heterotroph nitrifier: beef peptone 15g/L, NaCL5g/L, pH7.0: agar 20g/L, 25-35 ℃, pH is 7.0-7.2.
(2) efficiently repair composite fungus agent
The bacterial strain that composite fungus agent comprises is: bacterial strain is Rhod (Rhodococcus) (registering on the books and number CGMCC No.3276CN101748091 in the preservation center), Rhodopseudomonas (Pseudomonas) (registering on the books and number CGMCC No.3277CN101748091 in the preservation center), Saccharomycodes (Saccharomyces) (registering on the books and number CGMCC No.3278CN101748091 in the preservation center), micrococcus sp (Micrococcus luteus) (register on the books and number CGMCC No by the preservation center.3903CN102329744A）。After they remove COD and are respectively 88.3%, 83.1%, 77.5%, 76.2% 4 strain bacterium combination, treatment effect is 91.6%; Remove that TN is respectively 72.4%, 65.2%, 55.3%, after 53.8%. tetra-strain bacterium combinations, treatment effect is 74.8%, so this composite fungus agent has synergistic function.
What therefore in this research, finally use is to process black smelly river by the composite fungus agent by above-mentioned four strain bacterium combinations.Be the composite fungus agent that the black and odorous river river is administered.
Rhod (Rhodococcus CGMCC No.3276) Rhodopseudomonas (Pseudomonas CGMCCNo.3277) Saccharomycodes (Saccharomyces CGMCC No.3278) micrococcus sp (Micrococcus luteusCGMCC No.3903) ratio of four strain deodorant predominant bacterias in composite fungus agent is respectively 3:3:2:2.
The composite fungus agent substratum: yeast extract 10g/L, extractum carnis 5g peptone 10g/L,, MgSO
47H
2o20g/L, KCL2g/L, NaCL5g/L, pH7.0; 25-35 ℃.
(3) efficiently repair the on-the-spot enlarged culturing of composite fungus agent
Composite fungus agent at the scene during enlarged culturing, is adopted to the substratum of (2), by the needed amount in scene, carry out enlarged culturing.
(4) startup of on-the-spot biological treatment system, domestication, debugging, operation
On-the-spot needed composite fungus agent in (3) is added in combination biological restoration treatment system.The ratio that adds is relevant with COD concentration and the sewage quantity of sewage.Below divide three kinds of situations a) when COD concentration is less than 100mg/L, by the 1/100000(100,000 of system/) volumetric ratio added, i.e. 100000m
3add 1m in volume
3efficient reparation composite fungus agent.When b) COD concentration is less than 1000mg/L and is greater than 100mg/L, by the 1/10000-5/10000(ten thousand of system/5/10,000) volumetric ratio added, i.e. 10000m
3add 1m in volume
3-5m
3efficient reparation composite fungus agent.When c) COD concentration is greater than 1000mg/L, by 1/1000 volumetric ratio of system, added, in the 1000m3 volume, added 1m
3efficient reparation composite fungus agent.Simultaneously, regularly add the composite fungus agent substratum, make the growth that microbial inoculum is good at the biosystem energy and form microbial film, the unloading phase of having completed.
The unloading phase complete after, enter the domestication stage.That is: reduce gradually the amount that adds the composite fungus agent substratum and increase gradually black smelly river, until the dosage of composite fungus agent substratum is 0%, the amount of black smelly river is increased to 100%.
After starting and having tamed, just enter whole biosystem and equipment debugging, operation, finally make effluent quality reach the landscape water water quality standard.
Embodiment 2
1, turn river (containing The Taiping Lake), northern moat (pine forest lock-paper mill ship lock) wawter bloom Prevention and control project
Turning river, northern moat COD in water body, nitrogen, phosphorus etc. exceeds standard, water bloom pollution is serious, in order to solve this type of Pollution abatement, 2012, the efficient reparation composite fungus agent of embodiment 1 enlarged culturing is added and removes the COD that exceeds standard in water body, nitrogen, phosphorus etc. and (for adding front COD, nitrogen, phosphorus, transparency, be respectively 67mg/L, 31.2mg/L, 2.26mg/L, 30cm, add rear COD, nitrogen, phosphorus, transparency and be respectively 23mg/L, 7.4mg/L, 0.18mg/L, 70cm), make this water bloom of water body obtain effectively controlling.
2, river, Nansha, Beijing administration project up to standard
The severe overweights such as the COD exceeded standard in the river body of Nansha, Beijing in 2011, nitrogen, phosphorus, wawter bloom is serious and with black smelly phenomenon.For this type of Pollution abatement, the reparation composite fungus agent of embodiment 1 enlarged culturing is added and removes the COD that exceeds standard in water body, nitrogen, phosphorus etc. and (for adding front COD, nitrogen, phosphorus, transparency, be respectively 130mg/L, 41.3mg/L, 3.8mg/L, 20cm, add rear COD, nitrogen, phosphorus, transparency and be respectively 35mg/L, 9.8mg/L, 0.22mg/L, 60cm), increase the dissolved oxygen of water body simultaneously, its ecosystem is repaired fast.
3, the black smelly improvement of Qinhuangdao merchant river valley
10 days-July 20 June in 2009, start to add the reparation composite fungus agent of embodiment 1 enlarged culturing, 1 time is thrown the bacterium amount is 5 tons.Repair composite fungus agent after the 4th day when adding, black and stink disappear substantially, and COD, TN detected, TP has all reduced more than 70%.River water body is progressively recovering its natural self-purification capacity.
4, the black smelly improvement research in the river in river in the Liaocheng of Shandong Province Chi
In the Liaocheng City Chi, river is the passage of the south water to north, due to this ground industrial sewage and a large amount of the entering of sanitary sewage, directly have influence on the water quality of the south water to north, for the black smelly problem in river that solves weak middle river, 10 days-July 20 June in 2009, added the efficient reparation composite fungus agent of embodiment 1 enlarged culturing after the 7th day, considerable change has occurred in black and odorous river too, the black smelly effective improvement that obtains, water-quality COD is reduced to 70≤mg/L by 223mg/L, subdue COD0.453t/d, subdue rate 68.6%, the river course stinked to high heaven progressively recovers the natural self-purification capacity of water body.
Claims (6)
1. a composite fungus agent, is characterized in that, described composite fungus agent comprises bacterial strain:
Rhodococcus (Rhodococcus), deposit number CGMCC No.3276;
Pseudomonas (Pseudomonas), deposit number CGMCC No.3277;
Yeast (Saccharomyces), deposit number CGMCC No.3278;
Micrococcus sp (Micrococcus luteus), deposit number CGMCC No.3903.
2. composite fungus agent according to claim 1, is characterized in that, described rhodococcus, pseudomonas, yeast, the micrococci ratio in composite fungus agent is respectively 3:3:2:2.
3. the application of the described composite fungus agent of claim 1 in polluted-water is repaired.
4. a polluted-water aquatic ecosystem restorative procedure, is characterized in that, said method comprising the steps of:
(1) enlarged culturing composite fungus agent claimed in claim 1;
(2) drop in polluted-water.
5. polluted water body repairing method according to claim 4, is characterized in that, the enlarged culturing composite fungus agent is used the composite fungus agent substratum: yeast extract 10g/L, and extractum carnis 5g peptone 10g/L,, MgSO
47H
2o20g/L, KCL2g/L, NaCL5g/L, pH7.0.
6. polluted water body repairing method according to claim 4, is characterized in that, in step (2),
When a) water body COD concentration is less than 100mg/L, by 1/100000 volumetric ratio of system, added the reparation composite fungus agent;
When b) water body COD concentration is less than 1000mg/L and is greater than 100mg/L, by the 1/10000-5/10000 volumetric ratio of system, added composite fungus agent;
When c) water body COD concentration is greater than 1000mg/L, by 1/1000 volumetric ratio of system, added composite fungus agent.
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| CN105198160A (en) * | 2015-09-25 | 2015-12-30 | 重庆淏园环保科技有限公司 | Method for treating domestic sewage by aid of complex bacteria |
| CN105820985A (en) * | 2016-05-20 | 2016-08-03 | 张能力 | Compound microbial agent for efficiently treating sewage and preparation method of compound microbial agent |
| CN106045217A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Biological combined treatment method for rural domestic sewage |
| CN106045060A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Method for purifying domestic sewage by combination of granular multiple species inoculant and water plants |
| CN106365322A (en) * | 2016-05-25 | 2017-02-01 | 路域生态工程有限公司 | Method for highly efficiently treating black stinking water |
| CN109730093A (en) * | 2018-06-04 | 2019-05-10 | 北京东方昊天生物技术研究所有限公司 | A kind of aphid drives microbial inoculum and its preparation method and application |
| CN110698010A (en) * | 2019-09-22 | 2020-01-17 | 北京东方昊天生物技术研究所有限公司 | Application of rhodococcus |
| CN112011485A (en) * | 2020-08-26 | 2020-12-01 | 中国农业科学院植物保护研究所 | Rhodococcus pyridinivorans Rp3 with deodorizing and growth promoting effects and application thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105198160A (en) * | 2015-09-25 | 2015-12-30 | 重庆淏园环保科技有限公司 | Method for treating domestic sewage by aid of complex bacteria |
| CN105820985A (en) * | 2016-05-20 | 2016-08-03 | 张能力 | Compound microbial agent for efficiently treating sewage and preparation method of compound microbial agent |
| CN106365322A (en) * | 2016-05-25 | 2017-02-01 | 路域生态工程有限公司 | Method for highly efficiently treating black stinking water |
| CN106045217A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Biological combined treatment method for rural domestic sewage |
| CN106045060A (en) * | 2016-07-20 | 2016-10-26 | 申昱环保科技股份有限公司 | Method for purifying domestic sewage by combination of granular multiple species inoculant and water plants |
| CN109730093A (en) * | 2018-06-04 | 2019-05-10 | 北京东方昊天生物技术研究所有限公司 | A kind of aphid drives microbial inoculum and its preparation method and application |
| CN109730093B (en) * | 2018-06-04 | 2021-07-20 | 北京东方昊天生物技术研究所有限公司 | Aphid repellent agent and preparation method and application thereof |
| CN110698010A (en) * | 2019-09-22 | 2020-01-17 | 北京东方昊天生物技术研究所有限公司 | Application of rhodococcus |
| CN112011485A (en) * | 2020-08-26 | 2020-12-01 | 中国农业科学院植物保护研究所 | Rhodococcus pyridinivorans Rp3 with deodorizing and growth promoting effects and application thereof |
| CN112011485B (en) * | 2020-08-26 | 2022-04-08 | 中国农业科学院植物保护研究所 | Rhodococcus pyridinivorans Rp3 with deodorizing and growth promoting effects and application thereof |
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