CN106145383A - A kind of solid union microbial bacterial agent administered for black and odorous water - Google Patents

A kind of solid union microbial bacterial agent administered for black and odorous water Download PDF

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Publication number
CN106145383A
CN106145383A CN201610580989.7A CN201610580989A CN106145383A CN 106145383 A CN106145383 A CN 106145383A CN 201610580989 A CN201610580989 A CN 201610580989A CN 106145383 A CN106145383 A CN 106145383A
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CN
China
Prior art keywords
black
bacterial agent
microbial bacterial
odorous water
water
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Pending
Application number
CN201610580989.7A
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Chinese (zh)
Inventor
宋晨
刘晓玲
宋永会
廖苗
黄宇华
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Chinese Research Academy of Environmental Sciences
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Chinese Research Academy of Environmental Sciences
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Priority to CN201610580989.7A priority Critical patent/CN106145383A/en
Publication of CN106145383A publication Critical patent/CN106145383A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/14—NH3-N
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour

Abstract

The invention discloses a kind of solid union microbial bacterial agent administered for black and odorous water, it is mainly used in improving river, the black smelly and eutrophication of water body in lake, by Pichia sp. (Pichia manshurica), pseudomonas (Pseudomonas) forms with anthropi (Ochrobactrum), Pichia sp. in seed liquor, pseudomonas and anthropi ratio are followed successively by 1 1.5: 1: 1 1.5, after solution culture fermentation produces, add protective agent mixing, by centrifugal, filter and lyophilization mode collects thalline (moisture content is within 2%), microbial biomass reaches 5 × 107～6 × 107cells/g.The composite bacteria agent capable of the present invention is cheap, and thalline reproduction speed is fast, can be effectively improved the black smelly water quality with eutrophication water, effectively reduce COD in water body, NH3N, TP, water body chroma and smelly degree.

Description

A kind of solid union microbial bacterial agent administered for black and odorous water
Technical field
The present invention relates to water-treatment technology field, be specifically related to a kind of solid union microorganism administered for black and odorous water Microbial inoculum.
Background technology
Within 2014, China Environmental State Bulletin shows, 423 trunk rivers of China, the 968 of 62 emphasis lakes (reservoir) In individual state control surface water monitoring section (some position), the bad five class water quality sections of Haihe basin account for about 20%, and many of which water body occurs Seasonal or property black smelly phenomenon all the year round.The black smelly phenomenon of urban river not only result in the degeneration of river original function, and And had a strong impact on orthobiosis of nearby residents, just becoming the water environmental problems that China becomes increasingly conspicuous.Chinese Government is in 2015 April in year promulgates " water prevention and cure of pollution action plan ", proposes the target of regulation city black and odorous river the most by stages.
Improvement for this kind of water body both at home and abroad generally uses the technology such as Coagulation Method, biological floating bed, artificial swamp, the most right Improvement in black and odorous water obtains significant effect.But, these technology time-consumingly long, cost high, to the demand of soil space relatively Greatly, it is therefore desirable to a kind of simpler technological means efficiently processes demand with the river heavily contaminated that reply is increasingly severe.Utilize Microbial bacterial agent processes heavily contaminated river and is increasingly subject to extensive concern in recent years.Its effectiveness is mainly reflected in: 1. microbial bacteria In agent, Biomass is high, and the living bacteria count under unit mass or concentration generally reaches up to ten thousand the most more than one hundred million, can quickly form advantage Function strain, accelerates the degraded to pollutant or removal rate；2. microbial bacterial agent especially composite bacteria agent capable contains several functions Various pollutant complicated in river are shown extremely strong environmental resistance and specific aim by microorganism；3. microbial bacteria is added Agent can improve indigenous microorganism structure of community in water body, improves water body self-purification ability.Relative to Coagulation Method, biological floating bed, artificial The technology such as wetland, this technology is the shortest, convenient and efficient, low cost, takes up an area few.
The invention discloses a kind of solid union microbial bacterial agent administered for black and odorous water, be mainly used in improving river Stream, the black smelly and eutrophication of water body in lake, composite bacteria agent capable is cheap, and thalline reproduction speed is fast, can be effectively improved black The smelly water quality with eutrophication water, effectively reduces COD in water body, NH3-N, TP, water body chroma and smelly degree.
Summary of the invention
The invention discloses a kind of solid union microbial bacterial agent administered for black and odorous water, be mainly used in improving river Stream, the black smelly and eutrophication of water body in lake.
Technical scheme is as follows:
Screen from environment, separate, purification obtains Pichia sp., pseudomonas and anthropi, and is each closing respectively Enrichment culture in suitable culture medium, it is thus achieved that the seed liquor of each strain, by the seed liquor of Pichia sp., pseudomonas and anthropi Mix in the ratio of 1-1.5: 1: 1-1.5 successively, then seed mixture liquid is placed in fermenting and producing in fluid medium, cultivate basigamy Side be the Semen Maydis powder of 1%, the soybean cake powder of 3% and the fish flour of 3%, add after fermented production 1% sodium alginate, 1% glycerol and 1% beta-schardinger dextrin-, collects thalline after centrifugal, lyophilization, and Fungal biodiversity reaches 5 × 107～6 × 107cells/g。
The invention has the beneficial effects as follows: composite bacteria agent capable is cheap, thalline reproduction speed is fast, can be effectively improved black smelly and rich The water quality of nutritive water, effectively reduces COD in water body, NH3-N, TP, water body chroma and smelly degree.
Detailed description of the invention
With the obvious Shahe of the smelly phenomenon of Beijing Black as objective for implementation.
Screen from Shahe, separate, purification obtains Pichia sp., pseudomonas and anthropi, and is each closing respectively Enrichment culture in suitable culture medium, it is thus achieved that the seed liquor of each strain, by the seed liquor of Pichia sp., pseudomonas and anthropi Mixing in the ratio of 1-1.5: 1: 1-1.5 successively, the clump count of unit ratio is 8 × 105～9 × 105cells/gcells/mL。 Seed mixture liquid is placed in fermenting and producing in fermentation production medium, and culture medium prescription is Semen Maydis powder, the soybean cake powder of 3% of 1% With the fish flour of 3%, at 25 DEG C, pH is 7, and dissolved oxygen (DO) is fermenting and producing under the conditions of 2-4mg/L, adds 1% alginic acid afterwards Sodium, 1% glycerol and 1% beta-schardinger dextrin-, then collect thalline, moisture content control after centrifugal under the conditions of 4000rpm/min, lyophilization System is within 2%, and now Fungal biodiversity reaches 5 × 107～6 × 107cells/g.Thalline is thrown in black and odorous water, throw in Amount is 1%, and at 25 DEG C, pH is 7, reacts 24h, COD in water body, NH under the conditions of 120rpm/min shaking flask3-N, TP clearance are respectively Being 56%～61%, 74%～80% and 83%～85%, colourity drops to 2～4 times from 32～64 times, and smelly degree intensity is from 4～5 Drop to 0～1.

Claims (8)

1. the solid union microbial bacterial agent administered for black and odorous water, be mainly used in improving river, water body in lake black Smelly and eutrophication, by Pichia sp. (Pichia manshurica), pseudomonas (Pseudomonas) and pale bar Bacterium (Ochrobactrum) forms, and in seed liquor, Pichia sp., pseudomonas are followed successively by 1-1.5 with anthropi ratio: 1: 1- 1.5, after solution culture fermentation produces, add protective agent mixing, collect thalline by centrifugal, filtration and lyophilization mode (moisture content is within 2%), microbial biomass reaches 5 × 107～6 × 107cells/g。
A kind of solid union microbial bacterial agent administered for black and odorous water the most according to claim 1, it is characterised in that Described microbial bacterial agent form is solid-state.
A kind of solid union microbial bacterial agent administered for black and odorous water the most according to claim 1, it is characterised in that Described microbial bacterial agent is Bacterial Complex, and functional microorganism is Pichia sp., pseudomonas and anthropi.
A kind of solid union microbial bacterial agent administered for black and odorous water the most according to claim 1, it is characterised in that In described seed liquor, Pichia sp., pseudomonas are followed successively by 1-1.5 with anthropi ratio: 1: 1-1.5.
A kind of solid union microbial bacterial agent administered for black and odorous water the most according to claim 1, it is characterised in that The described Semen Maydis powder that liquid culture based formulas is 1%, the soybean cake powder of 3% and the fish flour of 3%, Semen Maydis powder, soybean cake powder and fish flour Ratio be 1: 3: 3.
A kind of solid union microbial bacterial agent administered for black and odorous water the most according to claim 1, it is characterised in that Described protective agent formula is 1% sodium alginate, 1% glycerol and 1% beta-schardinger dextrin-, sodium alginate, glycerol and beta-schardinger dextrin- Ratio is 1: 1: 1.
A kind of solid union microbial bacterial agent administered for black and odorous water the most according to claim 1, it is characterised in that Described solid-state microbial inoculum microbial biomass reaches 5 × 107～6 × 107cells/g。
A kind of complex micro organism fungicide administered for black and odorous water the most according to claim 1, it is characterised in that described Microbial bacterial agent be used for administering black and odorous water and eutrophication water.
CN201610580989.7A 2016-07-22 2016-07-22 A kind of solid union microbial bacterial agent administered for black and odorous water Pending CN106145383A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610580989.7A CN106145383A (en) 2016-07-22 2016-07-22 A kind of solid union microbial bacterial agent administered for black and odorous water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610580989.7A CN106145383A (en) 2016-07-22 2016-07-22 A kind of solid union microbial bacterial agent administered for black and odorous water

Publications (1)

Publication Number Publication Date
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107937298A (en) * 2017-06-08 2018-04-20 博元生态修复（北京）有限公司 A kind of complex micro organism fungicide, its preparation method and its application method administered for black and odorous water
CN109609401A (en) * 2018-12-18 2019-04-12 山东省科学院生态研究所 A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof
CN110885773A (en) * 2019-08-20 2020-03-17 中国环境科学研究院 Method for treating or purifying black and odorous water quality

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CN104630105A (en) * 2015-02-02 2015-05-20 李捍东 Composite microbial flocculant and application thereof

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107937298A (en) * 2017-06-08 2018-04-20 博元生态修复（北京）有限公司 A kind of complex micro organism fungicide, its preparation method and its application method administered for black and odorous water
CN107937298B (en) * 2017-06-08 2020-08-21 博元生态修复（北京）有限公司 Compound microbial agent for treating black and odorous water body, and obtaining method and using method thereof
CN109609401A (en) * 2018-12-18 2019-04-12 山东省科学院生态研究所 A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof
CN111117905A (en) * 2018-12-18 2020-05-08 山东省科学院生态研究所 Pichia mansoni
CN109609401B (en) * 2018-12-18 2020-05-12 山东省科学院生态研究所 Compound microbial deodorant bacterial agent and preparation method and application thereof
CN111117905B (en) * 2018-12-18 2022-03-22 山东省科学院生态研究所 Pichia mansoni
CN110885773A (en) * 2019-08-20 2020-03-17 中国环境科学研究院 Method for treating or purifying black and odorous water quality
CN110885773B (en) * 2019-08-20 2021-10-15 中国环境科学研究院 Method for treating or purifying black and odorous water quality

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Application publication date: 20161123