CN101701197A - Novel microorganism flora mixture and mixed nutrient medium thereof - Google Patents
Novel microorganism flora mixture and mixed nutrient medium thereof Download PDFInfo
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Abstract
The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.
Description
Technical field
The present invention relates to sewage treatment area, relate in particular to a kind of novel microorganism bacteria combing agent and substratum thereof.
Background technology
Current, China's problem of environmental pollution becomes increasingly conspicuous, and administers as water pollution and is just perplexing governments at all levels and all kinds of manufacturing enterprise.As everyone knows, with the method for physics (as salvaging) though can remove the part pollutent, but the processing to chemical pollutants such as ammonia nitrogen, nitrite and fowl and animal excrement etc. is difficult to prove effective, method with chemistry then easily causes secondary pollution, and physics with high costs, chemical treatment method are difficult to sewage is carried out advanced treatment.At present in China's environmental improvement engineering, all methods such as the activated sludge process that adopts, oxidation ditch, carborization more than 90%.These methods engineerings are complicated, investment is very big, working cost is high, and many areas, unit can not build or can not operate, and the mud of its generation is many in addition, has secondary pollution to solve again.Can not so taken very big financial resources, material resources, manpower, build up waste water treatment project and turn round or stagnant condition half more than 40% effectively to environment remediation.Along with science and technology development, microbiological manipulation is polluted technology and is come into one's own gradually in recent years, and is used widely in fields such as sewage disposals.Characteristics such as microorganism has that volume is little, surface-area is big, reproductivity is surprising, wide accommodation, effect are good.Sewage possesses the condition of microorganism growth breeding, thereby microorganism can obtain nutrient from sewage, degrades simultaneously and utilizes objectionable impurities, thereby sewage is purified.Microbial preparation mainly is made of organic microbial, and environment is safe from harm, and can also promote the rationalization and the high efficiency of biologic chain simultaneously.In developed countries such as the U.S., Japan and Europe, microbial preparation is widely used in the advanced treatment of wastewater of all trades and professions.China is since the just using microbe processing pollution twenties in last century, the scientific worker has also developed multiple microbial preparation, as disclosing a kind of complex micro organism fungicide among the Chinese patent literature CN 1189555C, but this type of complex microorganism preparations is failed all the time to break through and is difficult to expand rapidly that hyperplasia, microorganism are easily degraded, biological activity descends and the technical bottleneck of loss, is difficult to realize industrialization, commercialization such as microorganism.And the effect that using microbe is disposed of sewage largely depends on microbial numbers in the treatment system, composition and activity, existing this type of complex microorganism preparations bad adaptability, be difficult to bring back to life at short notice, breed, form the dominant microflora microbial film after entering water body, and subject range is little, and required time is long; And starting, dynamically cultivating and the dynamic operation management has inconvenience more, actual motion cost height, treatment effect be instability also.
Summary of the invention
The purpose of this invention is to provide a kind of novel microorganism flora mixed culture medium that can make microorganism species tame propagation rapidly, improve biological activity and improve the withstand load ability and effectively prevent biodeterioration, and a kind of novel microorganism bacteria combing agent that utilizes this substratum to inoculate and culture out is provided.
The technical scheme that technical solution problem of the present invention is adopted is:
According to the effect in the sewage material cycle transforms of the relation of biological and environmental pollutant, microorganism, the function and mechanism of of removal of microorganisms environmental pollutant, and take all factors into consideration alternate, the symbiotic relationship of each microorganism species, adopt suitable strain combinations, and the bacterial strain of the selected cultivation of foundation, add specific culture medium, improve the tolerance of dominant bacteria, cultivate, tame dominant microflora.
A kind of this mixed culture medium of novel microorganism flora mixed culture medium be by following raw material add wait to administer sewage or city integrated property sewage after, mixed fermentation to 80～88 ℃ and keep 3～6 days after, after filtration and filtrate:
Raw material weight per-cent
Plant powder 70～85%
Mineral substance 2～5%
Animal powder 2～15%
Organic acid 4～10%,
Described plant powder is the mixture of incense wood powder, rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, castor-oil plant shell powder; Described mineral substance is the mixture of Long Gu Powder, weathering stone flour, weathering brick powder, iron rust; Described animal powder is selected the mixture of shrimp shell meal, crab shell powder, oyster shells, people's hair powder, white glues powder for use; Described organic acid is selected the mixture of carboxylic acid, amino acid and Nucleotide for use; The weight ratio of used culture medium raw material gross weight and water is 1: 5～15.
Selected plant powder institute cellulose, xylogen and common plant cellulose, the wooden very big difference that have among the present invention, Mierocrystalline cellulose in the selected plant powder, content of lignin are than higher, at aspects such as fragrance quality its very big specificity is arranged, the plant powder of selected type can be used to cultivate the instructionization microorganism physiological function activity and withstand load ability, go stench ability, can cultivate instructionization and induce the variation of microbial enzyme, limpid, the no fishy smell of sewage, effluent quality of these bio-enzyme degradations by inducing instructionization is like mineral water and non-secondary pollution; The rotten wood powder mainly refers to the rotten wood powder that forms through the chemistry of micro-organisms effect under field conditions (factors) wherein include the strong degradation bacteria strains of strong, the anti-portative power of some vitality, introduces and cultivates propagation, can strengthen the degradation capability to sewage.
Long Gu Powder described in the present invention is meant the fossil meal of large-scale animal skeleton, and is cool in nature, detoxifcation life, anti-disease be used as medicine (seeing property of medicine opinion); Described weathering stone flour, weathering brick powder, land waste (awns stone), the brick powder of alkaline regional house, east, preferred Henan the foot of a wall, this area abounds with ground nitre (saltcake) can endure salt; They are weathering materials that natural weathering forms under microorganism participation effect, the class material is except that containing part calcium carbonate, also contain a large amount of saltpetre, sodium sulphate etc., this type of material can be used as medicine (seeing property of medicine opinion book), flavor is arduous, salty Great Cold, cure mainly abdomen account, extravesated blood, jaundice disease waiting time, be used to cultivate the physiological function activity of instructionization microorganism and anti-adversity ability (disease-resistant) and prevent that biological function from degenerating, and then strengthen degradation capability and the purification efficiency of flora various sewage.
Above-mentioned shrimp and crab shells powder claims chitin, shell element, chitin again, and especially chitinous content is higher all about 45%, far above the content of oyster shells 25%; Chitin is a kind of biological polymer of natural nitrogenous polyose that extensively is present in, it is a kind of biology cellulose of positively charged active group, it has special physicochemical property and biological activity, can effectively strengthen, reconcile the biological growth function, to preventing biological the degeneration Special Significance is arranged; Contain multiple amino acids and trace element in people's hair powder, the white glues powder is the deer horn rubber powder, the powder that the glue that boils for the angle of animal in deer family spotted deer or red deer grinds to form, people's hair powder, white glues powder be used as medicine (seeing property of medicine opinion), help life, can strengthen biological degeneration-resistant resistance against diseases, have vital role preventing biodeterioration.
Carboxylic acid (RCOOH) is a most important class organic acid, and in the saturated monocarboxylic acid, formic acid, acetate, propionic acid have strong tart flavour and pungency; Contain the corrupt stench of having of 4～9 C atoms, carboxylic acid can strengthen the anti-adversity ability of flora.Amino acid, Nucleotide, essential for microbial growth propagation, be to form DNA and RNA fundamental unit, in microorganism growth and biological metabolism, bringing into play important effect.
Municipal effluent is comprehensive sewage, promptly contain industrial sewage, also contain sanitary sewage etc., with municipal effluent allotment substratum material, fermentation is to filtration, be used further to cultivate the instructionization microorganism, can make microorganism species have more adaptability widely, extensively degradation capability, improve degradation speed and degradation efficiency, improve and water quality.Certainly also available treatment sewage is made corresponding substratum and is carried out specific aim cultivation, domestication.
Take all factors into consideration factors such as cost and domestication culture effect, the weight ratio of the incense wood powder in the described plant powder, rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, castor-oil plant shell powder is preferably 2: 2: 1: 4: 3: 2.
Described incense wood powder preferably originates in the mixture that northern Korean pine powder, Chinese pine powder preferably originate in southern Cortex cinnamomi camphorae powder, fragrant spring tree powder, and its optimum weight ratio is 2: 3: 2: 2.
Land waste, the weathering brick powder at the foundation place, old house of described weathering stone flour, alkalescence area, east, the preferred Henan of weathering brick powder or other zone similarity.
The optimum weight ratio of Long Gu Powder, weathering stone flour, weathering brick powder, iron rust is 2: 3: 3 in the described mineral: 5.
The weight ratio of shrimp shell meal, crab shell powder, oyster shells, people's hair powder, white glues powder is preferably 5: 4: 3 in the described plant powder: 2: 2.
The weight ratio preferably of carboxylic acid, amino acid, Nucleotide is 1: 3: 4～5 in the described organic acid.
A kind of novel microorganism bacteria combing agent, be from aerobic flora, anaerobic bacteria flora, the oxygen flora of holding concurrently, to optimize following bacterial strain or mushroom respectively after amplification culture step by step, again with each inoculation in the described novel microorganism flora of claim 1 mixed culture medium, the microbial preparation of making 20～45 ℃ of following mixed culture domestications 15～30 days; Contain in unit volume under the condition of approximately equal bacterium number, each bacterial strain population inoculum size is in parts by volume:
Bacterial strain population parts by volume
5～20 parts in sickle-like bacteria
5～20 parts in actinomycetes
5～20 parts of aspergillus tubigensis
5～10 parts of football shirt bacterium
1～15 part of osculum campanularian
5～40 parts of photosynthetic bacterias
1～15 part of methanobacteria
5～20 parts of desulfovibrios
1～17 part of clostridium
5～20 parts of bacterioides
5～20 parts of thermophilic sporeformer
1～20 part of bacillus capsulatus
1～13 part of cyanobacteria
1～10 part of nitrifier
1～15 part of Pseudomonas fluorescens
5～30 parts in yeast
5～20 parts of Xanthomonas campestris,
Grow is 1: 20～40 into the volume ratio of bacterial strain population total amount and mixed culture medium.
Photosynthetic bacteria (Photosynthetic Bacteria) be a class with light as the energy, can under anaerobism illumination or aerobic dark condition, utilize the organism of occurring in nature, sulfide, ammonia etc. carry out photosynthetic microorganism as the hydrogen donor carbon source of holding concurrently, can decompose absorption oxygen in a large number, objectionable impurities such as nitrogen and thiocyanogen, the change objectionable impurities is innoxious substance, and can suppress the procreation of oxygen consumption germ, the a large amount of carbohydrates of synthesis secretion, amino acids, biologically active substances such as VITAMIN (hormone) can also decompose harmful substances in air, sewage there is very strong detergent power, it and actinomycetes, coordinative role is arranged between the microorganisms such as milk-acid bacteria, occurring in nature can be with the bacterium of photosynthesis production capacity, according to producing oxygen photosynthetic bacterium and non-oxygen-production photosynthetic bacterium different being divided into of their contained photosynthetic pigments and electron donor; Actinomycetes (Actinomycete) are aerobic bacteria, are the intermediaries of bacterium and mould, its growth adaptation temperature is 23～37 ℃, the amino acid that its picked-up photosynthetic bacteria produces, produce antibiotics, suppress pathogenic bacteria, bacterium such as harmful mould propagation desired substance (chitin) increases thereby suppress it, creates the environment of beneficial microorganism growth; Yeast (Yeast) can produce and promote bacterium splitted biologically active substance, and the secretory product of its generation can be by photosynthetic bacteria synthesizing amino acid and carbohydrate; Methanobacteria (Methanogensbacteria) is the anaerobic main bacteria seed, a large amount of methane of pollution degradation deposits yields, and nitrated clearance is the indicative biology of anaerobic degradation more than 85%～90% in anaerobic reactor; Football shirt bacterium (Sphaerotilus) football shirt bacterium is a kind of filamentous bacterium with typical structure, extensively be present in active sludge and the contaminated water body, its oxidisability height, can make full use of multiple organism degrades, particularly carbide is as nutrition, still growing multiplication is fast in little oxygen environment, and capacity of decomposition is strong, at 1000mm
2In can absorb 30% organism; Sickle-like bacteria (Fusarium) but at occurring in nature decomposition of cellulose degradation of organic substances, play a part certain to natural material cycle, at the biological eliminating oxynitride, biological degradation phenolic compound, prussiate and synthetic dyestuff, absorb, accumulate, there is great potential aspect such as degrading polycyclic aromatic hydrocarbons, and environment protection is played very important strength; Bacillus capsulatus, dendritic glue Pseudomonas, self energy forms flco, strengthens sediment, and materials such as benzene, phosphorus, phenol are had stronger capacity of decomposition, also can effectively improve sludge activity.
Pseudomonas fluorescens (P.Fluorescens), Xanthomonas campestris (Xanthomonas oryzae), the ability that all has stronger thermotolerance, radioprotective, anti-chemicals is strong, the metabolism complexity is various, is the main bacteria seed of falling repugnant substance to playing a major role in the conversions such as carbon, nitrogen, thionin; Nitrifier is a kind of aerobic bacteria, can in the water of oxygen is arranged or in the gravel, grow, and in nitrogen cycle purification of water quality process, playing the part of important role, they comprise a kind of bacillus, coccus and the spiral type bacterium of form inequality type, a class that belongs to absolute self-operation microorganism comprises nitrous acid Pseudomonas (Nitrosomonas), nitric acid Pseudomonas (Nitrobacter); Bacteroideae (Bacteroides), gram negative bacilli, different in size, be polymorphism, thalline often has irregular expansion, can form pod membrane, no gemma, atrichia, obligate anaerobic has higher removal ability to starch based sewage; Cyanobacteria, prokaryotic organism, the water body purification ability is strong, and it is vigorous to grow in the sewage that nitrogen, phosphorus enrich, and degradation capability is strong, is the indicative biology of water nutritionization; Osculum campanularian (V.microstoma), original microorganism, Ciliata section, happiness is in the polluted water environment, and active high resistant power is stronger, can engulf unwanted bacteria in a large number, and clearance is 1000mm
270%, to self purification of water body degree height, be the indicative biology of biological sewage treatment; Desulfovibrio (Desulfovibriodesulfuricans), active high in anaerobic process, the removal pollutent of can effectively degrading is accelerated the nitrated degraded of anaerobism, improves and accelerates degradation speed and degradation efficiency; Bacillus capsulatus (Clostridium perfringens) is that anaerobism, unpowered, the thick bacillus of Gram-positive that can produce the brood cell are as judging that whether soil is by the indicator of fecal pollution and pollution level.
When above-mentioned novel microorganism bacteria combing agent uses, put into to wait to administer and carry out sewage disposal in the sewage and purify according to the weight ratio that practical situation can 3/10000ths to 8/10000ths.
The present invention has the following advantages and the high-lighting effect:
1. the creative allotment mixed culture based raw material of selecting constitutes, by mixed culture medium of the present invention cultivate, the domestication dominant microflora, can improve in the composition flora to the tolerance of poor environment.Domestication is to make microorganism species progressively adapt to certain specified conditions by the artificial measures, obtains to have the flora of higher tolerance and metabolic activity at last.By adaptive process, for the strange compound of microorganism can be induced the synthetic of essential degrading enzyme; Or owing to new enzyme system is set up in spontaneous mutation; Or significantly change its phenotype, and carry out self-metabolism and regulate, come the Degradation and Transformation pollutent.The present invention adds specified raw material material (as weathering stone flour, iron rust, rotten trees powder etc.) in the substratum medium, microbial community structure is changed towards the direction that is adapted to the sewage envrionment conditions, can strengthen biological physiology function and activity effectively, both improve biological rate of propagation, and can prevent the degeneration of microorganism species again effectively.
2. from aerobic flora, anaerobic bacteria flora, the oxygen flora of holding concurrently, choose the optimum combination bacterial strain, dissolve bacteria combing agent through mixed culture medium cultivation propagation of the present invention, instruction with flora combination advantage, its flora monolithic stability, be difficult for variation, adaptability is strong, has broken through that microorganism species in the common micro-organisms compound formulation is easily degraded, biological activity easily descends, hyperplasia is expanded technical bottleneck slowly.
3. have stronger metabolism and cometabolism ability between each bacterial strain in the novel microorganism bacteria combing agent of the present invention, much than the organism of difficult degradation, by microorganism different strain group's interaction, cometabolism changes its chemical structure; To a kind of organic substance, when a kind of flora can not be degraded, other flora can be coordinated degraded; This Synergistic degradation function and effect have improved greatly or have established the using value of this microorganism composition in environment protection, improvement pollution engineering, have widened Application Areas.
4. the novel microorganism bacteria combing agent of the inventive method being produced combines with traditional biological pollution treatment treatment facility and both economized on resources, can effectively improve its processing efficiency again, solution traditional technology operation stability is poor, required time long, an inefficient difficult problem.It is cheap that this efficient microbe bacteria combing agent adds expense, can ramp after rendering to it in sewage, breeding, can form microbially decontaminate film (existing complex microorganism preparations forms the time of microbial film needs more than 4 each months) generally speaking in 40～50 days, to polluting dirty clearance up to 96～98.5%, aquifer yield matter height, limpid tasteless, and nontoxic, non-secondary pollution significantly reduces sludge yield; This bacteria combing agent is applied to engineering when disposing of sewage annually only needs augmented injection 2～3 times, and filling in per 3～4 months once gets final product, and does not need successive to annotate, and operation cost greatly reduces.
5. economy, social value height, novel microorganism bacteria combing agent of the present invention combines with traditional biological pollution treatment treatment facility and disposes of sewage, investment and running cost are low, and investment is 50～60% of a domestic popular oxidation ditch process engineering, can save 40～50% investment; 5 fens water per ton of per ton need of operating charges can be saved 0.5～2.5 yuan, also saves about 3 yuan/ton of water resources taxes simultaneously.
6. novel microorganism bacteria combing agent of the present invention is applied widely, can handle various trade effluents, city integrated waste water, also directly treatment and purification swag, lake, river make Heisui River paleness water, and do not have stench flavor, flow to and where where be purified to, effectively realize natural purification and restoration of the ecosystem.
Embodiment
Embodiment 1 laboratory city domestic sewage Processing Test
Test is handled used city domestic sewage and is derived from Zhengzhou City's sewage network, drops into novel microorganism bacteria combing agent 0.12kg/m to sewage
3, forming bio-film colonization after 15 days, the water quality clarification continues to drop into this microbe bacteria combing agent 0.02kg/m in the protection period
3Water quality detection before and after test is handled the results are shown in subordinate list 1.
Above-mentioned novel microorganism bacteria combing agent production stage is as follows:
(1) by weight percentage, (weight ratio of incense wood powder, rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, castor-oil plant shell powder is 1: 2: 2: 4 to get the plant powder; 3: 2) 70%, 5) 5%, after animal powder (weight ratio of shrimp shell meal, crab shell powder, oyster shells, people's hair powder, white glues powder is 4: 4: 3: 2: 2) 15%, organic acid (weight ratio of carboxylic acid, amino acid and Nucleotide is 1: 3: 4) 10% add city integrated property sewage by 1: 20 weight ratio (weight ratio of Long Gu Powder, weathering stone flour, weathering brick powder, iron rust is 2: 3: 3 to mineral substance:, mixed fermentation to 80 ℃ and keep 6 days after, make liquid nutrient medium after the filtration; Above-mentioned incense wood powder is Korean pine powder, Chinese pine powder, Cortex cinnamomi camphorae powder, fragrant spring tree powder with weight ratio 2: 3: 3: 2 mixture;
(2) will be step by step the following bacterial strain of amplification culture by volume part be inoculated in the above-mentioned mixed culture medium, 35 ℃ down in domestication after breeding 20 days, grow is 1: 20 into the volume ratio of bacterial strain population total amount and mixed culture medium:
5 parts in sickle-like bacteria, 20 parts in actinomycetes, 10 parts of aspergillus tubigensis, 10 parts of football shirt bacterium, 15 parts of osculum campanularians, 10 parts of photosynthetic bacterias, 5 parts of methanobacterias, 55 parts of desulfovibrios, 2 parts of clostridium, 5～20 parts of bacterioides, 5 parts of thermophilic bacilluss, 20 parts of bacillus capsulatuses, 5 parts of cyanobacterias, 2 parts of nitrifiers, 15 parts of Pseudomonas fluorescens, 30 parts in yeast, 5 parts of Xanthomonas campestris.
Embodiment 2 mixed industrial wastewater treatment tests
Get the mixed industrial waste water of a plurality of factories in suburbs, Zhengzhou discharging, drop into this novel microorganism bacteria combing agent 0.09kg/m to waste water
3, form bio-film colonization after 20 days, the water quality clarification, the water quality detection before and after handling the results are shown in subordinate list 2.
Above-mentioned novel microorganism bacteria combing agent production stage is as follows:
(1) by weight percentage, get plant powder (incense wood powder, the rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, the weight ratio of castor-oil plant shell powder is 2: 2: 1: 4: 3: 2) 80%, mineral substance (Long Gu Powder, the weathering stone flour, weathering brick powder, the weight ratio of iron rust is 2: 3: 3: 5) 3%, animal powder (shrimp shell meal, crab shell powder, oyster shells, people's hair powder, the weight ratio of white glues powder is 5: 4: 3: 2: 2) 10%, organic acid (carboxylic acid, the weight ratio of amino acid and Nucleotide is 1: 3: 4～5) 7% add this mixed industrial waste water by 1: 13 weight ratio after, mixed fermentation to 85 ℃ and keep 4 days after, make liquid nutrient medium after the filtration; Above-mentioned incense wood powder is Korean pine powder, Chinese pine powder, Cortex cinnamomi camphorae powder, fragrant spring tree powder with weight ratio 2: 3: 2: 2 mixture;
(2) will be step by step the following bacterial strain of amplification culture by volume part be inoculated in the above-mentioned mixed culture medium, 25 ℃ down in domestication after breeding 15 days, grow is 1: 30 into the volume ratio of bacterial strain population total amount and mixed culture medium:
15 parts in sickle-like bacteria, 15 parts in actinomycetes, 10 parts of aspergillus tubigensis, 8 parts of football shirt bacterium, 9 parts of osculum campanularians, 20 parts of photosynthetic bacterias, 8 parts of methanobacterias, 16 parts of desulfovibrios, 9 parts of clostridium, 10 parts of bacterioides, 10 parts of thermophilic bacilluss, 10 parts of bacillus capsulatuses, 7 parts of cyanobacterias, 5 parts of nitrifiers, 8 parts of Pseudomonas fluorescens, 20 parts in yeast, 12 parts of Xanthomonas campestris.
The new prosperous Paper Co., Ltd in embodiment 3 Xinmi City recycled writing paper sewage treatment project
Ton surplus the said firm's year production boxboard 17000,1000～1500 tons of day effluents, the waste water COD content 1800～2100mg/L that is discharged in its production process, BOD
5Mean value is up to 568mg/L, adopt the present invention in conjunction with artificial swamp paper waste to be carried out advanced treatment in February, 2006, its treatment process is: throw in microbial inoculum and carry out pre-treatment in raw wastewater, enter the coagulative precipitation tank post precipitation through grid again, enter the aeration tank aerobic oxidation then, add again and add the processing of effectively compound microbial bacteria combing agent, the processing of last supernatant liquor introducing artificial swamp.Build up from test 40 days up to standard of the water outlet of intaking, the microbially decontaminate film forms, and water outlet exceeds the quata up to standard.Checking and accepting the total clearance of COD through monitoring is 95.7%, and the BOD clearance is 93.8%, sees table 3 for details.
Above-mentioned novel microorganism bacteria combing agent production stage is as follows:
(1) by weight percentage, get plant powder (incense wood powder, the rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, the weight ratio of castor-oil plant shell powder is 3: 2: 1: 4: 3: 2) 85%, mineral substance (Long Gu Powder, the weathering stone flour, weathering brick powder, the weight ratio of iron rust is 2: 3: 3: 5) 2%, animal powder (shrimp shell meal, crab shell powder, oyster shells, people's hair powder, the weight ratio of white glues powder is 5: 4: 3: 2: 2) 5%, organic acid (carboxylic acid, the weight ratio of amino acid and Nucleotide is 1: 3: 4～5) 8% add city integrated property sewage by 1: 5～20 weight ratio after, mixed fermentation to 80 ℃ and keep 6 days after, make liquid nutrient medium after the filtration; Above-mentioned incense wood powder is Korean pine powder, Chinese pine powder, Cortex cinnamomi camphorae powder, fragrant spring tree powder with weight ratio 3: 3: 2: 2 mixture;
(2) will be step by step the following bacterial strain of amplification culture by volume part be inoculated in the above-mentioned mixed culture medium, 40 ℃ down in domestication after breeding 25 days, grow is 1: 20 into the volume ratio of bacterial strain population total amount and mixed culture medium:
20 parts in sickle-like bacteria, 20 parts in actinomycetes, 20 parts of aspergillus tubigensis, 10 parts of football shirt bacterium, 15 parts of osculum campanularians, 5～40 parts of photosynthetic bacterias, 15 parts of methanobacterias, 20 parts of desulfovibrios, 17 parts of clostridium, 20 parts of bacterioides, 20 parts of thermophilic bacilluss, 20 parts of bacillus capsulatuses, 13 parts of cyanobacterias, 10 parts of nitrifiers, 15 parts of Pseudomonas fluorescens, 30 parts in yeast, 20 parts of Xanthomonas campestris.
Embodiment 4 mayors of Xinmi City win Paper Co., Ltd's recycled writing paper sewage treatment project
The said firm's main products is a boxboard, and a year designed productive capacity is 11000～20000 tons, 800～1000 tons of day effluents.The waste water COD that is discharged in its production process
CrMean value is up to 1700mg/L, BOD
5Mean value is up to 611mg/L, adopt in April, 2005 the present invention paper waste to be carried out advanced treatment in conjunction with " northern vertical subsurface flow formula artificial swamp technological system ", its treatment process is: after raw wastewater enters the processing of the biochemical pool, throw microbial inoculum and enter contact-oxidation pool, under the effect of aerobic microbiological, organism in oxidation, the degrading waste water, contact-oxidation pool water outlet gravity flow enters inclined-plate clarifying basin and carries out precipitation process, and supernatant liquor is handled from flowing to artificial swamp.Pending paper waste pH value is transferred to 6～8, quantitatively throw in effectively compound microbial bacteria combing agent 0.08g/m at contact-oxidation pool, artificial swamp water inlet respectively
3, the proliferate enough rapidly of input microbial strain group energy, and depend on wetland dielectric material carrier fast, in 45 days, form stable microbial film network test water outlet success, move so far, effluent quality is COD 57mg/L, and becomes better and better.
Xinmi City's Bureau for Environmental Protection monitoring that carries out an acceptance inspection on June 26th, 2006; and invite Environmental Protection Agency of provinces and cities to participate in examination; monitoring by inspection; influent waste water COD content is 800～1500mg/L; water outlet COD is 57mg/L; BOD is 22mg/L, and clearance COD, BOD are 96.6%, 96.4% (monitoring data sees Table 4).
Above-mentioned novel microorganism bacteria combing agent production stage is as follows:
The following bacterial strain of amplification culture step by step by volume part is inoculated in the mixed culture medium described in the embodiment 1,20 ℃ down in domestication after breeding 30 days, grow is 1: 20 into the volume ratio of bacterial strain population total amount and mixed culture medium.
5 parts in sickle-like bacteria, 5 parts in actinomycetes, 5 parts of aspergillus tubigensis, 5 parts of football shirt bacterium, 1 part of osculum campanularian, 5 parts of photosynthetic bacterias, 1 part of methanobacteria, 5 parts of desulfovibrios, 1 part of clostridium, 5 parts of bacterioides, 5 parts of thermophilic bacilluss, 1 part of bacillus capsulatus, 1 part of cyanobacteria, 1 part of nitrifier, 1 part of Pseudomonas fluorescens, 5 parts in yeast, 5 parts of Xanthomonas campestris.
Embodiment 5 Xinxiang City Hailin, Yuanyang starch factory sewage treatment project
800～1000 tons of this factory's day effluents, DOD content is 12000mg/L, adopt artificial wetland technology system in the north and biological flora composition of the present invention that factory effluent is carried out advanced treatment, A/O technology is adopted in pre-treatment, be 39.5mg/L (see Table 5) through the monitoring water outlet COD of Xinxiang City Environmental Protection Agency in June, 2008, clearance is 98.7%, and its water outlet has been used to breed fish.
Used biological flora composition and substratum are identical with embodiment 1.
Table 1 novel microorganism bacteria combing agent treatment effect (embodiment 1)
Table 2 novel microorganism bacteria combing agent treatment effect (embodiment 2)
Table 3 each processing unit clearance is checked and accepted and is analyzed (embodiment 3)
Table 4 each processing unit clearance is checked and accepted and is analyzed (embodiment 4)
Table 5 environmental monitoring station analytical results (embodiment 5)
Claims (8)
1. a novel microorganism flora mixed culture medium is characterized in that, this mixed culture medium be by following raw material add wait to administer sewage or city integrated property sewage after, mixed fermentation to 80～88 ℃ and keep 3～6 days after, after filtration and filtrate:
Raw material weight per-cent
Plant powder 70～85%
Mineral substance 2～5%
Animal powder 2～15%
Organic acid 4～10%,
Described plant powder is the mixture of incense wood powder, rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, castor-oil plant shell powder; Described mineral substance is the mixture of Long Gu Powder, weathering stone flour, weathering brick powder, iron rust; Described animal powder is selected the mixture of shrimp shell meal, crab shell powder, oyster shells, people's hair powder, white glues powder for use; Described organic acid is selected the mixture of carboxylic acid, amino acid and Nucleotide for use; The weight ratio of used culture medium raw material gross weight and water is 1: 5～15.
2. according to the described novel microorganism flora of claim 1 mixed culture medium, it is characterized in that the weight ratio of incense wood powder, rotten wood powder, peanut hull meal, Semen Sesami Nigrum shell powder, black soya bean shell powder, castor-oil plant shell powder is 2: 2: 1 in the described plant powder: 4: 3: 2.
3. according to the described novel microorganism flora of claim 1 mixed culture medium, it is characterized in that described incense wood powder is the mixture of Korean pine powder, Chinese pine powder, Cortex cinnamomi camphorae powder, fragrant spring tree powder, its weight ratio is 2: 3: 2: 2.
4. according to any described novel microorganism flora of the claim mixed culture medium of claim 1 to 3, it is characterized in that described weathering stone flour, weathering brick powder are selected land waste, the weathering brick powder at foundation place, alkaline regional house, east, Henan for use.
5. according to the described novel microorganism flora of claim 4 mixed culture medium, it is characterized in that the weight ratio of Long Gu Powder, weathering stone flour, weathering brick powder, iron rust is 2: 3: 3 in the described mineral: 5.
6. according to the described novel microorganism flora of claim 5 mixed culture medium, it is characterized in that the weight ratio of shrimp shell meal, crab shell powder, oyster shells, people's hair powder, white glues powder is 5: 4: 3 in the described plant powder: 2: 2.
7. according to the described novel microorganism flora of claim 6 mixed culture medium, it is characterized in that the weight ratio of described carboxylic acid, amino acid, Nucleotide is 1: 3: 4～5.
8. novel microorganism bacteria combing agent, it is characterized in that, it is to optimize following bacterial strain or mushroom respectively from aerobic flora, anaerobic bacteria flora, the oxygen flora of holding concurrently after enlarged culturing step by step, again it is inoculated in the described novel microorganism flora of claim 1 mixed culture medium made microbial preparation after 20～45 ℃ of following mixed culture are tamed 15～30 days; Contain in unit volume under the condition of approximately equal bacterium number, each bacterial strain population inoculum size is in parts by volume:
Bacterial strain population parts by volume
5～20 parts in sickle-like bacteria
5～20 parts in actinomycetes
5～20 parts of aspergillus tubigensis
5～10 parts of football shirt bacterium
1～15 part of osculum campanularian
5～40 parts of photosynthetic bacterias
1～15 part of methanobacteria
5～20 parts of desulfovibrios
1～17 part of clostridium
5～20 parts of bacterioides
5～20 parts of thermophilic sporeformer
1～20 part of bacillus capsulatus
1～13 part of cyanobacteria
1～10 part of nitrifier
1～15 part of Pseudomonas fluorescens
5～30 parts in yeast
5～20 parts of Xanthomonas campestris,
Grow is 1: 20～40 into the volume ratio of bacterial strain population total amount and mixed culture medium.
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Cited By (6)
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| CN105039234A (en) * | 2015-07-10 | 2015-11-11 | 中国海洋大学 | Microbial complex flora for eliminating sulfide in sediments |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
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| CN108718579A (en) * | 2018-04-19 | 2018-11-02 | 郭婷 | A kind of soil remediation method that resource utilization is high |
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| JP2004248618A (en) * | 2003-02-21 | 2004-09-09 | Hoomaa Clean Kk | Bacterial group symbiotically living with fungus used for treating organic material and its application |
| CN101029298A (en) * | 2006-12-11 | 2007-09-05 | 河南亚神环保科技有限公司 | Production of efficient microbe bacteria combing agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105039234A (en) * | 2015-07-10 | 2015-11-11 | 中国海洋大学 | Microbial complex flora for eliminating sulfide in sediments |
| CN105039234B (en) * | 2015-07-10 | 2019-02-01 | 中国海洋大学 | It is a kind of for eliminating the microbial composite bacteria group of sulfide in deposit |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
| CN106754514B (en) * | 2016-12-23 | 2019-08-13 | 钟华 | A kind of sustainable dredging removes the microbial bacterial agent preparation method of black and odorous water |
| CN108502963A (en) * | 2018-04-02 | 2018-09-07 | 苏斌 | A kind of sewage-treating agent and its method for handling black and odorous water |
| CN108611099A (en) * | 2018-04-19 | 2018-10-02 | 郭婷 | A kind of soil-repairing agent that resource utilization is high |
| CN108718579A (en) * | 2018-04-19 | 2018-11-02 | 郭婷 | A kind of soil remediation method that resource utilization is high |
| CN114409095A (en) * | 2022-01-13 | 2022-04-29 | 湖南省环境保护科学研究院 | Method for treating aquaculture tail water by using immobilized photosynthetic bacteria flora |
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