CN109055252A - A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations and preparation method thereof - Google Patents
A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations and preparation method thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
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- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The present invention relates to a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations, it is characterised in that: the raw material of the complex microorganism preparations is vibrio alginolyticus and pseudomonas aeruginosa, and two kinds of bacterium mix post-fermentation finished product.In the present invention, using vibrio alginolyticus HA2 and pseudomonas aeruginosa YY24 as raw material, finished product after mixed fermentation, it is directly proportionally added in aeration tank when use, nitrification and denitrification process is carried out simultaneously, part nutriment needed for two kinds of bacterium provide growth each other, culture is not necessarily to special expensive inducing substance and condition is extremely simple, greatly reduce the cost of industrialized production, and it will limit the pH value that anti-nitration reaction rises water body when nitration reaction, keep the pH value of water body relatively stable, and it is high to the intracorporal ammonia nitrogen removal frank of water, action time is short, it will not influence fish, shrimp, the eco-activity of plant etc., it can be in common fresh water soil pond, batch production fresh water/seawater workshop, sanitary sewage, faintly acid water body, it is used in the exceeded water body of the nitrogen contents such as industrial wastewater.
Description
Technical field
The present invention relates to water body tri-state nitrogen processing technology field, especially a kind of heterotrophic nitrification-aerobic denitrification is compound micro-
Biological agent and preparation method thereof.
Background technique
Three kinds of states existing for N element in water: nitrate nitrogen (nitrate form), nitrite nitrogen (nitrite anions) and ammonium nitrogen
(ammonium root), these substances have a certain impact to the growth and development of material culture in water body, once reaching a certain concentration, then can support
It grows in object and stockpiles, influence the health of the mankind indirectly, or even material culture can be made dead.
Traditional water process theory thinks: the nitrification and denitrification two mutually independent processes that are removed by of ammonia nitrogen are realized
, since the requirement to environmental condition is different, the two processes cannot occur simultaneously, and can only list type carry out, i.e., nitrification is anti-
It should occur under aerobic condition, anti-nitration reaction then occurs under stringent anoxic or anaerobic condition.Under this theoretical direction,
Traditional biological denitrification process is all to separate anoxic zone (or anaerobic zone) with aerobic zone, such as A/O system.It is supplied oxygen in aerobic zone
Abundance, ammonia nitrogen are oxidized to nitrate nitrogen by nitrifying bacteria community, and then mixed liquor is generally back to forward type anoxic section；In anoxic item
Under part, denitrifying bacterium completes denitrification process using the organic matter in nitrate nitrogen and raw sewage, achievees the purpose that denitrogenation.It is above-mentioned
Denitrification process process procedure is more, and processing cost is high, the time is long, is unfavorable for controlling water body ammonia-nitrogen content rapidly, in rear-mounted anti-nitre
Change occasion because in mixed liquor organic matter shortage, generally also need manually to add carbon source.
With the development of bio-denitrification technology theory, it is de- that nitrification bacteria, aerobic denitrifying bacteria etc. are different from traditional biological
The discovery of nitrogen bacterium, so that heterotrophic nitrification-aerobic denitrification denitrogenation becomes reality.Biological denitrificaion work based on new denitrogenation theory
Skill is also emerged in large numbers one after another, wherein be widely applied using bacillus as the biological denitrification process of dominant bacteria in Japan, South Korea, it is close several
Year is also gradually applied in the Practical Projects such as the newly-built, reorganization and expansion of China's town sewage plant.
Summary of the invention
It is total the purpose of the present invention is to provide nitrification-denitrification two processes, bacterial strains can be carried out simultaneously under aerobic conditions
It is anti-that the OH- that the product of nitrification after life can be released directly as the substrate and denitrification of denitrification can partially compensate nitrification
Alkali consumed by answering simultaneously makes the metastable a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations of pH value in system.
The technical solution adopted by the present invention is that:
A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations, it is characterised in that: the complex microorganism preparations
Raw material is vibrio alginolyticus and pseudomonas aeruginosa, and two kinds of bacterium mix post-fermentation finished product.
Moreover, the ratio of the vibrio alginolyticus and pseudomonas aeruginosa inoculation is that (optical density is for 1:2.31~5.52
OD600)。
Moreover, the entitled vibrio alginolyticus HA2 of the vibrio alginolyticus, belongs to vibrio alginnolyficus kind, Latin literary fame is known as
Vibrio alginolyticus, depositary institution are China General Microbiological culture presevation administrative center, deposit number CGMCC
No.9115, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Moreover, the entitled pseudomonas aeruginosa YY24 of the pseudomonas aeruginosa, belongs to P. aeruginosa strain, draw
Fourth literary fame is known as Pseudomonas aeruginosa, and depositary institution is China General Microbiological culture presevation administrative center, protects
Hiding number is CGMCC No.9114, and preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
It is a further object to provide a kind of preparation sides of heterotrophic nitrification-aerobic denitrification complex microorganism preparations
Method, it is characterised in that: it is seeded in culture medium after mixing vibrio alginolyticus and pseudomonas aeruginosa, it is complete after cultivating a period of time
At fermentation；
The entitled vibrio alginolyticus HA2 of the vibrio alginolyticus, belongs to vibrio alginnolyficus kind, Latin literary fame is known as Vibrio
Alginolyticus, depositary institution are China General Microbiological culture presevation administrative center, deposit number CGMCC
No.9115, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；
The entitled pseudomonas aeruginosa YY24 of the pseudomonas aeruginosa, belongs to P. aeruginosa strain, Latin
Entitled Pseudomonas aeruginosa, depositary institution are China General Microbiological culture presevation administrative center, and preservation is compiled
Number be CGMCC No.9114, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Moreover, two plants of bacterium of the vibrio alginolyticus and pseudomonas aeruginosa mix (optical density with the ratio of 1:2.31~5.52
For OD600), inoculated and cultured amount is 100~130 with 1~5% inoculation of culture volume, 23~35 DEG C of cultivation temperature, revolving speed
Turn, pH value is 4~9.
Moreover, incubation time is 20~30 hours, culture OD value is detected, has been fermented when OD value is to 2.15 ± 0.02
At.
Moreover, the culture medium includes KH2PO4、MgSO4·7H2O、Na2HPO4And microelement, adjust pH be 7.0~
7.5。
It is a further object to provide a kind of users of heterotrophic nitrification-aerobic denitrification complex microorganism preparations
Method, it is characterised in that: 1~3 ‰ complex microorganism preparations Jing Guo freeze-drying process are added in staying water, after addition
Carry out aeration oxygen replenishing.
Another object of the present invention is a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations in water body tri-state nitrogen
Application in processing.
The advantages and positive effects of the present invention are:
In the present invention, using vibrio alginolyticus HA2 and pseudomonas aeruginosa YY24 as raw material, finished product after mixed fermentation makes
Used time is directly proportionally added in aeration tank, while carrying out nitrification and denitrification process, and two kinds of bacterium provide growth institute each other
Part nutriment is needed, culture is not necessarily to special expensive inducing substance and condition is extremely simple, greatly reduces industrialized production
Cost, and will limit the pH value that anti-nitration reaction rises water body when nitration reaction, keep the pH value of water body relatively stable, and right
The intracorporal ammonia nitrogen removal frank of water is high, and action time is short, will not influence the eco-activity of fish, shrimp, plant etc., can be in common fresh water soil
Make in the exceeded water bodys of nitrogen contents such as pond, batch production fresh water/seawater workshop, sanitary sewage, faintly acid water body, industrial wastewater
With.
Specific embodiment
Below with reference to embodiment, the present invention is further described, following embodiments be it is illustrative, be not restrictive,
It cannot be limited the scope of protection of the present invention with following embodiments.
A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations, innovation of the invention are: the complex microorganism
The raw material of preparation is vibrio alginolyticus and pseudomonas aeruginosa, and two kinds of bacterium mix post-fermentation finished product.
The ratio of inoculation when above-mentioned vibrio alginolyticus and the pseudomonas aeruginosa are fermented is 1:2.31~5.52 (optical density
For OD600).
Wherein, the entitled Vibrio alginolyticus HA2 of the vibrio alginolyticus, belongs to vibrio alginnolyficus kind, Latin title
For Vibrio alginolyticus, depositary institution is China General Microbiological culture presevation administrative center, and deposit number is
CGMCC No.9115, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, April 30 2014 preservation time.Institute
The entitled pseudomonas aeruginosa YY24 for stating pseudomonas aeruginosa, belongs to P. aeruginosa strain, Latin literary fame is known as
Pseudomonas aeruginosa, depositary institution are China General Microbiological culture presevation administrative center, and deposit number is
CGMCC No.9114, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, April 30 2014 preservation time.
The preparation method of above-mentioned heterotrophic nitrification-aerobic denitrification complex microorganism preparations is: vibrio alginolyticus and verdigris is false
It is seeded in culture medium after monad mixing, after cultivating a period of time, completes fermentation；The vibrio alginolyticus and pseudomonas aeruginosa
Two plants of bacterium are mixed with the ratio of 1:2.31~5.52, and inoculated and cultured amount is with 1~5% inoculation of culture volume, cultivation temperature 23
~35 DEG C, revolving speed be 100~130 turns, pH value is 4~9.It is even more preferred that incubation time is 20~30 hours, then detection training
Object OD value is supported, fermentation is completed when OD value is to 2.15 ± 0.02.
The culture medium prescription are as follows: (g/L):
Tryptone 10；Yeast extract 5；NaCl 10；NH4Cl 0.5；Sodium citrate 5.66；Vickers salting liquid 50mL,
The seawater (NaCl 15) that salinity is 15 ‰ adjusts pH to 7.0.
Vickers salting liquid (g/L): K2HPO45.0；MgSO4·7H2O 2.5；FeSO4·7H2O 0.05；MnSO4·4H2O
0.05。
A kind of application method of heterotrophic nitrification-aerobic denitrification complex microorganism preparations is: 1 being added in staying water
~3 ‰ complex microorganism preparations Jing Guo freeze-drying process carry out aeration oxygen replenishing after addition.
The screening process of two kinds of bacterium is:
1.2012 years, in Tianjin, the sea-farming filtering ponds of Li Da seawater resources development corporation, Ltd. carried out adopting for water sample
Collection obtains two plant heights and imitates denitrifier through TanJin Agricultural College marine products institute Tianjin aquatic ecosystem and the screening of cultivation key lab.
2. carrying out the test of denitrification ability for 24 hours of two plants of bacterium, experimental condition is: with bacterium solution 1% in the case where testing room environmental
Ratio is seeded in the 250ml conical flask of 100ml, and obtained data are: pseudomonas aeruginosa YY24 is under the best growing condition
Ammonia nitrogen removal frank for 24 hours can reach 96.67%, and denitrification percent reaches 95.36%；Vibrio alginolyticus HA2 is under the best growing condition
Ammonia nitrogen removal frank for 24 hours is up to 97.24%, and denitrification percent is up to 90.92%.
By screening, determine that two plants of bacterium have the advantages that denitrification ability is strong and has carried out biological deposits.
The salinity that the compound formulation microorganism adapts to is very wide, is applicable in water body: light including common fresh water soil pond, batch production
The exceeded water body of the nitrogen contents such as water/seawater workshop, sanitary sewage, faintly acid water body, industrial wastewater.
NO- 2- N or NO- 3- N is the direct product of nitration reaction, but in NH+ 4While-N is degraded, entire biology is de-
Nitrogen process can't detect the product, shows nitrification and denitrification reaction while carrying out.In reaction process, two plants of bacterial strain symbiosis can
Product NO to generate some growth needed nutrient matter each other, during nitration reaction- 2- N and NO- 3- N can be directly as
The substrate of denitrification is reduced into N in conjunction with organic matter2.Because denitrifying bacterium growth and breeding is fast, therefore avoid nitration product
Inhibition of the accumulation of nitric acid, nitrous acid to nitration reaction, accelerates nitration denitrification process, and denitrification percent is apparently higher than other trainings
The method of supporting, denitrogenation fast speed.
Nitration reaction pH is 7.0~8.0, and basicity should maintain 70mg/L or more, therefore individually carry out nitration reaction, basicity
When inadequate, alkali should be also supplemented, and the OH released in two plants of bacterial strain symbiosis anti-nitration reactions-Nitration reaction can partially be compensated to be disappeared
The alkali of consumption, while part combines the H generated during nitration reaction+, pH value is relatively stable in maintenance system, is maintained at pH
7.0~7.5.
Vibrio alginolyticus, P. aeruginosa growth denitrogenation need (ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, simple organic with nitrogen source
Nitrogen), carbon source (sodium citrate), few calcium and magnesium manganese as microelement based on.
Embodiment 1
1. vibrio alginolyticus and pseudomonas aeruginosa are mixed with the ratio of 1:2.31；
2. culture medium composition is:
Culture medium prescription is (g/L): tryptone 10；Yeast extract 5；NaCl 10；NH4Cl 0.5；Sodium citrate
5.66；Vickers salting liquid 50mL, the seawater (NaCl 15) that salinity is 15 ‰ adjust pH to 7.0.
Vickers salting liquid (g/L): K2HPO45.0；MgSO4·7H2O 2.5；FeSO4·7H2O 0.05；MnSO4·4H2O
0.05。
3. being 100~130 in 25 degrees Celsius of temperature, revolving speed by the mixture of step 1 by 5% inoculation of culture volume
It is cultivated under conditions of rev/min, pH value is 7.0~7.5；
4. detecting culture OD value after culture 20 hours, when OD value is to 2.15 ± 0.02, fermentation is completed.
Embodiment 2
1. vibrio alginolyticus and pseudomonas aeruginosa are mixed with the ratio of 1:5.52；
2. culture medium composition is:
Culture medium prescription is (g/L): tryptone 10；Yeast extract 5；NaCl 10；NH4Cl 0.5；Sodium citrate
5.66；Vickers salting liquid 50mL, the seawater (NaCl 15) that salinity is 15 ‰ adjust pH to 7.0.
Vickers salting liquid (g/L): K2HPO45.0；MgSO4·7H2O 2.5；FeSO4·7H2O 0.05；MnSO4·4H2O
0.05。
3. being 100~130 in 35 degrees Celsius of temperature, revolving speed by the mixture of step 1 by 1% inoculation of culture volume
It is cultivated under conditions of rev/min, pH value is 7.0~7.5；
4. detecting culture OD value after culture 30 hours, when OD value is to 2.15 ± 0.02, fermentation is completed.
Embodiment 3
1. vibrio alginolyticus and pseudomonas aeruginosa are mixed with the ratio of 1:3；
2. culture medium composition is:
Culture medium prescription is (g/L): tryptone 10；Yeast extract 5；NaCl 10；NH4Cl 0.5；Sodium citrate
5.66；Vickers salting liquid 50mL, the seawater (NaCl 15) that salinity is 15 ‰ adjust pH to 7.0.
Vickers salting liquid (g/L): K2HPO45.0；MgSO4·7H2O 2.5；FeSO4·7H2O 0.05；MnSO4·4H2O
0.05。
3. being 100~130 in 30 degrees Celsius of temperature, revolving speed by the mixture of step 1 by 3% inoculation of culture volume
It is cultivated under conditions of rev/min, pH value is 7.0~7.5；
4. detecting culture OD value after culture 30 hours, when OD value is to 2.15 ± 0.02, fermentation is completed.
Embodiment 4
1. vibrio alginolyticus and pseudomonas aeruginosa are mixed with the ratio of 1:3.7；
2. culture medium composition is:
Culture medium prescription is (g/L): tryptone 10；Yeast extract 5；NaCl 10；NH4Cl 0.5；Sodium citrate
5.66；Vickers salting liquid 50mL, the seawater (NaCl 15) that salinity is 15 ‰ adjust pH to 7.0.
Vickers salting liquid (g/L): K2HPO45.0；MgSO4·7H2O 2.5；FeSO4·7H2O 0.05；MnSO4·4H2O
0.05。
3. being 100~130 in 30 degrees Celsius of temperature, revolving speed by the mixture of step 1 by 3% inoculation of culture volume
It is cultivated under conditions of rev/min, pH value is 7.0~7.5；
4. detecting culture OD value after culture 20 hours, when OD value is to 2.15 ± 0.02, fermentation is completed.
The effect that above-mentioned complex microorganism preparations are applied to when water body is handled can be referring to comparative test.
Comparative test:
The water body that experimental group, blank control group and positive controls use is that quantitative nitrogenous dirt is added in sterile water
Object is contaminated, 1 ‰ complex microorganism preparations are added in experimental group, 1 ‰ common ammonia nitrogen removal agent is added in positive controls,
Blank control group is not processed, by 12h, for 24 hours, after 36h, 48h, 72h distinguish measurement experiment group, positive controls and blank pair
According to total nitrogen in group (TN), ammonia nitrogen (TAN), nitrite nitrogen (NO2-N), nitrate nitrogen (NO3-N) content, concrete outcome is shown in Table 1~
4。
Table 1: total nitrogen (TN) content in water body
Table 2: ammonia nitrogen (TAN) content in water body
Table 3: nitrite nitrogen (NO in water body2-- N) content
Table 4: nitrate nitrogen in water body (NO3-- N) content
Conclusion: total nitrogen (TN), ammonia nitrogen (TAN), nitrous state by table 1,2,3,4 it is found that when 72 hours, in experimental group
Nitrogen (NO2-- N), nitrate nitrogen (NO3-- N) content is well below control group, and treatment effect is better than positive controls.
In the present invention, using vibrio alginolyticus HA2 and pseudomonas aeruginosa YY24 as raw material, finished product after mixed fermentation makes
Used time is directly proportionally added in aeration tank, while carrying out nitrification and denitrification process, and two kinds of bacterium provide growth institute each other
Part nutriment is needed, culture is not necessarily to special expensive inducing substance and condition is extremely simple, greatly reduces industrialized production
Cost, and will limit the pH value that anti-nitration reaction rises water body when nitration reaction, keep the pH value of water body relatively stable, and right
The intracorporal ammonia nitrogen removal frank of water is high, and action time is short, will not influence the eco-activity of fish, shrimp, plant etc., can be in common fresh water soil
Make in the exceeded water bodys of nitrogen contents such as pond, batch production fresh water/seawater workshop, sanitary sewage, faintly acid water body, industrial wastewater
With.
Claims (10)
1. a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations, it is characterised in that: the original of the complex microorganism preparations
Material is vibrio alginolyticus and pseudomonas aeruginosa, and two kinds of bacterium mix post-fermentation finished product.
2. a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations according to claim 1, it is characterised in that: institute
The ratio for stating inoculation when vibrio alginolyticus and pseudomonas aeruginosa fermentation is 1:2.31~5.52.
3. a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations according to claim 1 or 2, it is characterised in that:
The entitled vibrio alginolyticus HA2 of the vibrio alginolyticus, belongs to vibrio alginnolyficus kind, Latin literary fame is known as Vibrio
Alginolyticus, depositary institution are China General Microbiological culture presevation administrative center, deposit number CGMCC
No.9115, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
4. a kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations according to claim 1 or 2, it is characterised in that:
The entitled pseudomonas aeruginosa YY24 of the pseudomonas aeruginosa, belongs to P. aeruginosa strain, Latin literary fame is known as
Pseudomonas aeruginosa, depositary institution are China General Microbiological culture presevation administrative center, and deposit number is
CGMCC No.9114, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
5. a kind of preparation method of heterotrophic nitrification-aerobic denitrification complex microorganism preparations, it is characterised in that: by vibrio alginolyticus and
It is seeded in culture medium after pseudomonas aeruginosa mixing, after cultivating a period of time, completes fermentation；
The entitled vibrio alginolyticus HA2 of the vibrio alginolyticus, belongs to vibrio alginnolyficus kind, Latin literary fame is known as Vibrio
Alginolyticus, depositary institution are China General Microbiological culture presevation administrative center, deposit number CGMCC
No.9115, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；
The entitled pseudomonas aeruginosa YY24 of the pseudomonas aeruginosa, belongs to P. aeruginosa strain, Latin title
For Pseudomonas aeruginosa, depositary institution is China General Microbiological culture presevation administrative center, and deposit number is
CGMCC No.9114, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
6. a kind of preparation method of heterotrophic nitrification-aerobic denitrification complex microorganism preparations according to claim 5, special
Sign is: two plants of bacterium of the vibrio alginolyticus and pseudomonas aeruginosa are mixed with the ratios of 1:2.31~5.52, inoculated and cultured amount with
1~5% inoculation of culture volume, 23~35 DEG C of cultivation temperature, revolving speed are 100~130 turns, pH value is 4~9.
7. a kind of preparation method of heterotrophic nitrification-aerobic denitrification complex microorganism preparations according to claim 5 or 6,
It is characterized by: incubation time is 20~30 hours, culture OD value is detected, fermentation is completed when OD value is to 2.15 ± 0.02.
8. a kind of preparation method of heterotrophic nitrification-aerobic denitrification complex microorganism preparations according to claim 7, special
Sign is: every liter of culture medium contains following by a gram component for weight calculation, comprising: tryptone 10, yeast extract 5, and NaCl 10,
NH4Cl 0.5, sodium citrate 5.66, Vickers salting liquid 50mL adjust pH to 7.0.
9. a kind of application method of heterotrophic nitrification-aerobic denitrification complex microorganism preparations, it is characterised in that: in staying water
It is middle that 1~3 ‰ complex microorganism preparations Jing Guo freeze-drying process are added, aeration oxygen replenishing is carried out after addition.
10. a kind of application of heterotrophic nitrification-aerobic denitrification complex microorganism preparations in the processing of water body tri-state nitrogen.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112299566A (en) * | 2019-07-24 | 2021-02-02 | 天津农学院 | New method for removing nitrogen and phosphorus |
| CN113528382A (en) * | 2021-07-05 | 2021-10-22 | 中国成套设备进出口集团有限公司 | Construction and application of composite denitrification microbial inoculum capable of utilizing low-cost carbon source |
| CN114853182A (en) * | 2022-05-07 | 2022-08-05 | 福建工程学院 | Method for treating zinc ion pollution in water body based on microbial denitrification |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB847640A (en) * | 1957-07-31 | 1960-09-14 | Carves Simon Ltd | Improvements relating to the treatment of industrial effluents |
| CN102876616A (en) * | 2012-10-17 | 2013-01-16 | 北京大学 | Pseudomonas aeruginosa for denitrification and phosphorous removal under different dissolved oxygen conditions and application of pseudomonas aeruginosa |
| CN104480045A (en) * | 2014-12-17 | 2015-04-01 | 深圳市深港产学研环保工程技术股份有限公司 | Efficient aerobic denitrifying strain having heterotrophic nitrification function and application of strain |
| CN106047742A (en) * | 2016-04-18 | 2016-10-26 | 天津农学院 | Vibrio alginolyticus strain and application of same to denitrification of water body |
| CN106906170A (en) * | 2017-05-03 | 2017-06-30 | 上田环境修复股份有限公司 | Complex micro organism fungicide and its preparation method and application |
| CN107164276A (en) * | 2017-06-21 | 2017-09-15 | 北京大学 | One plant tolerance zinc ion toxicity pseudomonas aeruginosa and its application |
| CN107244742A (en) * | 2017-05-10 | 2017-10-13 | 上田环境修复股份有限公司 | A kind of method of the microbial degradation of pollutant in trade effluent |
-
2017
- 2017-11-13 CN CN201711113925.7A patent/CN109055252A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB847640A (en) * | 1957-07-31 | 1960-09-14 | Carves Simon Ltd | Improvements relating to the treatment of industrial effluents |
| CN102876616A (en) * | 2012-10-17 | 2013-01-16 | 北京大学 | Pseudomonas aeruginosa for denitrification and phosphorous removal under different dissolved oxygen conditions and application of pseudomonas aeruginosa |
| CN104480045A (en) * | 2014-12-17 | 2015-04-01 | 深圳市深港产学研环保工程技术股份有限公司 | Efficient aerobic denitrifying strain having heterotrophic nitrification function and application of strain |
| CN106047742A (en) * | 2016-04-18 | 2016-10-26 | 天津农学院 | Vibrio alginolyticus strain and application of same to denitrification of water body |
| CN106906170A (en) * | 2017-05-03 | 2017-06-30 | 上田环境修复股份有限公司 | Complex micro organism fungicide and its preparation method and application |
| CN107244742A (en) * | 2017-05-10 | 2017-10-13 | 上田环境修复股份有限公司 | A kind of method of the microbial degradation of pollutant in trade effluent |
| CN107164276A (en) * | 2017-06-21 | 2017-09-15 | 北京大学 | One plant tolerance zinc ion toxicity pseudomonas aeruginosa and its application |
Non-Patent Citations (1)
| Title |
|---|
| IMELDA JOSEPH、V CHANDRIKA: "Biodegradation of phenol using bacteria from different brackishwater habitats", 《INDIAN JOURNAL OF MARINE SCIENCES》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112299566A (en) * | 2019-07-24 | 2021-02-02 | 天津农学院 | New method for removing nitrogen and phosphorus |
| CN113528382A (en) * | 2021-07-05 | 2021-10-22 | 中国成套设备进出口集团有限公司 | Construction and application of composite denitrification microbial inoculum capable of utilizing low-cost carbon source |
| CN114853182A (en) * | 2022-05-07 | 2022-08-05 | 福建工程学院 | Method for treating zinc ion pollution in water body based on microbial denitrification |
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