CN105585133A

CN105585133A - Bio-denitrification method for high-salt-content wastewater discharged from catalyst production process

Info

Publication number: CN105585133A
Application number: CN201410585644.1A
Authority: CN
Inventors: 李宝忠; 高会杰; 孙丹凤; 郭志华
Original assignee: China Petroleum and Chemical Corp; Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Current assignee: China Petroleum and Chemical Corp; Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Priority date: 2014-10-28
Filing date: 2014-10-28
Publication date: 2016-05-18
Anticipated expiration: 2034-10-28
Also published as: CN105585133B

Abstract

The invention discloses a bio-denitrification method for high-salt-content wastewater discharged from a catalyst production process. In the method, active sludge from a wastewater treatment plant is employed as a carrier, and under the condition that MLSS is lower than 5000 mg/L, nitrifying bacteria and a microorganism growth promoter are added. When nitrite nitrogen in the system is more than 50% of total nitration products, a denitrifying bacteria agent and the microorganism growth promoter are added to the system. When the concentrations of ammonia nitrogen and total nitrogen are both lower than 50 mg/L, the starting step is finished and the system goes into stable operation. The microorganism growth promoter includes, by weight, 40-100 parts of metal salts and 5-30 parts of a polyamine substance, preferably 50-80 parts of the metal salts and 10-20 parts of the polyamine substance. The metal salts include calcium salt, copper salt, magnesium salt and/or ferrous salt. In the method, the nitrifying bacteria and the denitrifying bacteria agent are employed as biological main bodies, while the microorganism growth promoter is supplied for improving growth rate of microorganisms and improving wastewater treatment efficiency. The method solves a problem of treating the high-salt-content wastewater discharged from the catalyst production process in the prior art.

Description

The biological denitrification method of the high saline sewage of Catalyst Production process discharge

Technical field

The invention belongs to environmental project dirty water living creature processing technique field, be specifically related to a kind of biological denitrification method of high saline sewage of Catalyst Production process discharge.

Background technology

In the Catalyst Production process of petrochemical industry, many places are used ammonium salt and ammoniacal liquor, therefore in the containing wastewater from catalyst of discharge, contain a large amount of ammonia nitrogens. Although the ammonia nitrogen in waste water can adopt the physico-chemical processes such as stripping stripping, ion-exchange, chemical oxidation to process, there is accessory substance secondary pollution and the problem such as treatment effeciency is low in these methods. By contrast, bioanalysis is the better method of controlling water body ammonia and nitrogen pollution.

Traditional biological method is as the terminal technology of conventional sewage disposal, limited in one's ability in the time processing high ammonia nitrogen, low COD waste water, usually sacrifices load to realizing the qualified discharge of ammonia nitrogen pollutant in waste water. Particularly in the time that salinity is higher in sewage, can suppress microbial growth and reduce the activity of microorganism, bringing certain difficulty to biological treatment. Although all carried out repeatedly a large amount of improvement from the aspect such as technique and sewage treatment structure, in sewage disposal process, play certain effect, but because the main body activated sludge of being responsible for ammonia nitrogen removal does not change, so the removal effect of ammonia nitrogen is not still very desirable. The ammonia nitrogen problem that exceeds standard directly has influence on the comprehensive up to standard and normal production of Catalyst Production enterprise wastewater, becomes the bottleneck of restriction enterprise development, Ammonia Wastewater Treatment is become to the primary environmental issue of Catalyst Production enterprise. Therefore researching and developing economic, practical, the safe treatment technology containing ammonia nitrogen in salt catalyst wastewater becomes current study hotspot, to protection of the environment, promote the well-being of mankind significant.

No matter be traditional or novel bio-denitrification technology, increasing biomass is one of effective means improving sewage treating efficiency. Along with some novel, effective denitrification microorganisms, as allotrophic nitrobacteria, aerobic denitrifying bacteria, anaerobic ammonium oxidizing bacteria etc., separated qualification is out in succession, biological reinforcing technology is widely applied, and people have carried out a large amount of research work to bacteria agent.

CN101302485A discloses a kind of heterotrophic nitrification microbial preparation, its cultural method and purposes, and this microbial inoculum can effectively remove ammonia nitrogen and the total nitrogen in water body, can also remove the COD in organic wastewater simultaneously, is applicable to high-concentration culture waste water processing. CN200910021020.7 discloses a kind of preparation method of the water quality modifying microecological preparation that falls ammonia nitrogen and nitrous acid nitrogen, and this invention probiotics belongs to aquaculture technology and ecological environmental protection technical field. In the denitrogenation processing of the high saline sewage that mentioned microorganism microbial inoculum produces in Catalyst Production process, result of use is limited, need to develop suitable denitrogenation microbial inoculum and method of wastewater treatment for high saliferous Catalyst Production sewage. CN201210102760.4 discloses the processing method of a kind of high salinity, high-concentration ammonia nitrogenous wastewater, and the method adopts the rear nitrobacteria of domestication, is applicable to processing ammonia nitrogen concentration 500mg/L, salt content 35g/L with interior high salinity waste water. CN201210130653.2 discloses a kind of high biological denitrification method containing salt catalyst sewage, and by adding in sewage, nitrobacteria and the denitrogenation microbial inoculum taking nitrite as electron acceptor realize. Ammonia nitrogen concentration and salt content that above-mentioned processing method can tolerate are limited, and need to add a large amount of denitrogenation microbial inoculums to overcome the inhibition of salt content to microbial activity.

Summary of the invention

For the deficiency of prior art existence, the invention provides a kind of biological denitrification method of high saline sewage of Catalyst Production process discharge. The method is using the nitrobacteria denitrogenation microbial inoculum close with growth conditions as biological body, employing is added growth of microorganism promoter and is improved microbial growth speed and improve sewage treating efficiency, has solved the processing problem of the high saliferous ammonia-containing water producing in existing Catalyst Production process.

The biological denitrification method of the high saline sewage of Catalyst Production process discharge of the present invention, comprise following content: using sludge sewage as microbe carrier, activated sludge concentration lower than 5000mg/L condition under, preferably activated sludge concentration is under 2000～4000mg/L condition, to add nitrobacteria and growth of microorganism promoter, when nitrite nitrogen in system accounts for the more than 50% of total nitration product, while being preferably 50%～80%, add denitrification microbial inoculum and growth of microorganism promoter, when ammonia nitrogen and total nitrogen concentration are all lower than 50mg/L, during preferably lower than 25mg/L, startup stage, finishes, enter steady running operation, described growth of microorganism promoter comprises slaine and polyamines, and wherein slaine is 40 ~ 100 weight portions, is preferably 50 ~ 80 weight portions, and polyamines is 5 ~ 30 weight portions, is preferably 10 ~ 20 weight portions, described slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt.

Slaine in growth of microorganism promoter of the present invention can be calcium salt, magnesium salts and mantoquita, wherein Ca²⁺、Mg²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (0.5 ~ 5), is preferably (8 ~ 12): (10 ~ 20): (1 ~ 4); Or calcium salt, ferrous salt and mantoquita, wherein Ca²⁺、Fe²⁺And Cu²⁺Mol ratio be (5 ~ 15): (1 ~ 8): (0.5 ~ 5), is preferably (8 ~ 12): (2 ~ 6): (1 ~ 4); Or calcium salt, magnesium salts, ferrous salt and mantoquita, wherein Ca²⁺、Mg²⁺、Fe²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (1 ~ 8): (0.5 ~ 5), is preferably (8 ~ 12): (10 ~ 20): (2 ~ 6): (1 ~ 4).

Calcium salt in growth of microorganism promoter of the present invention is CaSO₄Or CaCl₂, preferably CaSO₄; Magnesium salts is MgSO₄Or MgCl₂, preferably MgSO₄; Ferrous salt is FeSO₄Or FeCl₂, preferably FeSO₄; Mantoquita is CuSO₄Or CuCl₂, preferably CuSO₄. Polyamines in described growth of microorganism promoter is spermine, spermidine or both mixtures.

Growth of microorganism promoter of the present invention can also comprise inorganic acid azanol, and content is 0.5 ~ 15 weight portion, is preferably 2 ~ 10 weight portions. Described inorganic acid azanol is one or more in hydroxylamine hydrochloride, HAS or phosphatic hydroxylamine, is preferably HAS. Can be used as the adding in right amount of inorganic acid azanol azanol oxygen also the matrix of enzyme participate in directly nitrobacteria metabolic process, shorten enzymatic reaction process, simultaneously can accelerated cell growth as the activator of cell.

The water quality characteristic of high saline sewage of the present invention is: ammonia nitrogen concentration is generally 30 ~ 600mg/L, and COD(Cr method is lower same) be 30 ~ 1000mg/L, electrical conductivity is 5 ~ 60ms/cm, Cu²⁺Concentration is 2 ~ 50mg/L, Zn²⁺Concentration is 2 ~ 100mg/L, SS(suspension) be 10 ~ 1000mg/L, pH is 6 ~ 10.

In biological denitrification method of the present invention, sludge sewage can be from oil refining sewerage treatment plant, catalyst sewage treatment plant or municipal wastewater treatment plant etc. The temperature of controlling sewage disposal is 18-40 DEG C, is preferably 25-35 DEG C, and dissolved oxygen is 0.1 ~ 4.0mg/L, is preferably 0.2 ~ 3.0mg/L, and pH is 7.0-9.0, is preferably 7.5-8.5.

The nitrobacteria that the present invention adds comprises Nitromonas and Nitrosomas, and preferably Nitrosomas accounts for the more than 60% of nitrobacteria total quantity, ensures that the nitrite nitrogen of reaction system accounts for the more than 50% of total nitration product. Nitrobacteria can adopt various existing schemes to cultivate, and also can adopt commercially available microbial inoculum, as the nitrosation dominant microflora that can obtain according to CN201010221166.8.

The present invention use denitrification microbial inoculum contain Staphylococcus cohnis (Staphylococcuscohnii) FSDN-C, arthrobacterium (Arthrobactercreatinolyticus) FDN-1 and Shui Shi Flavobacterium (Flavobacteriummizutaii) at least one in FDN-2; Three kinds of bacterial strains are respectively on July 14th, 2011 and on March 11st, 2010 and be preserved in that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", deposit number is respectively CGMCCNO.5062, CGMCCNo.3657, CGMCCNo.3659. Above-mentioned thalline after can cultivating separately, mix in proportion more also can be directly Mixed culture in proportion, preferably direct Mixed culture in proportion.

Growth of microorganism promoter of the present invention need coordinate nitrobacteria and denitrification microbial inoculum to add simultaneously. The dosage of growth of microorganism promoter adds according to promoter concentration 10 ~ 40mg/L in sewage disposal system, and preferably 20 ~ 30mg/L adds. Nitrobacteria dosage is that 30 ~ 100mg/L adds according to MLSS. Add after nitrobacteria, can add denitrification microbial inoculum when nitrite nitrogen in system accounts for 50% of total nitration product when above. Nitrobacteria can disposablely add also and can add in batches, when ammonia nitrogen concentration in waste water can stop adding during lower than 50mg/L. Denitrification microbial inoculum need add in batches, preferably divides and adds for 3 ~ 10 times, adds once every 1～7 day. Add by the volume ratio that accounts for the amount of disposing of sewage 0.001% ~ 0.1% per hour first, successively successively decrease, each dosage successively decreases 30%～50% than last time later, when total nitrogen concentration stops adding during lower than 50mg/L. After adding thalline, sewage disposal system can not spoil disposal in 3 months. For intermittently operated technique, the amount of disposing of sewage per hour is average treatment amount.

The biological denitrification method of the high saline sewage of the Catalyst Production process discharge that the present invention proposes is mainly that the optimum organization by directly adding microorganism in growth of microorganism promoter cooperation nitrobacteria and denitrification microbial inoculum, reinforcement sludge, the group status of change denitrification microorganism are realized.

The present invention adds the growth of microorganism promoter of special composition and proportioning in wastewater treatment process, make added nitrobacteria and denitrogenation microbial inoculum under the acting in conjunction of slaine, polyamines and inorganic acid azanol, realize the fast breeding of cell, can fast degradation substrate, and can improve the salt resistant character of cell and the stability of system, extend thalline service life. Being used in conjunction with of described different microorganism and growth promoter, can reduce the dosage of nitrobacteria and denitrogenation microbial inoculum, high salinity in the sewage producing in can tolerant catalyst production process, system strong shock resistance, can maintain the stable operation of system, effect when ammonia nitrogen and TN removel rate any microorganism when not adding promoter is used separately.

Detailed description of the invention

Further illustrate by the following examples process and the effect of the inventive method.

The nitrobacteria that embodiment uses cultivates by CN201010221166.8 embodiment 1 method the nitrosation dominant microflora obtaining. The denitrification microbial inoculum using adopts and carries out denitrifying microbial inoculum with nitrite described in CN201010536004.3, preferably contain simultaneously Staphylococcus cohnis (Staphylococcuscohnii) FSDN-C, arthrobacterium (Arthrobactercreatinolyticus) FDN-1 and Shui Shi Flavobacterium (Flavobacteriummizutaii) microbial inoculum prepared of tri-kinds of bacterial strains of FDN-2.

The preparation of embodiment 1 growth of microorganism promoter

The preparation method of growth of microorganism promoter of the present invention is: (1) prepares metal salt solution according to following composition and weight portion: slaine is 40 ~ 100 weight portions, be preferably 50 ~ 80 weight portions, described slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt; (2) use is front by 5 ~ 30 weight portions, and the polyamines that is preferably 10 ~ 20 weight portions joins in metal salt solution. Further, before use, can also add content is 0.5 ~ 15 weight portion, is preferably the inorganic acid azanol of 2 ~ 10 weight portions.

Adopt said method according to the growth of microorganism promoter of the ratio of table 1 promoter and four kinds of models of formula preparation, described promoter concentration is 0.5g/L.

The formula of table 1 promoter and ratio

The preparation of embodiment 2 denitrification microbial inoculums

1, Staphylococcus cohnis FSDN-C of the present invention, arthrobacterium FDN-1 and Shui Shi Flavobacterium FDN-2 being inoculated in respectively on beef extract-peptone solid medium is to activate in 30 DEG C of constant incubators in temperature, culture medium prescription is: beef extract: 5g/L, peptone: 10g/L, NaNO₂: 1g/L, add 2.0% agar, wherein in Staphylococcus cohnis FSDN-C solid medium, add 0.5mL/L methyl alcohol. The condition of culture of bacterial strain is for being that 30 ~ 35 DEG C, 150 ~ 240rpm concussion are cultured to logarithmic phase and can gather in the crops thalline for the preparation of microbial bacterial agent in temperature.

2, scrape a ring solid colony inoculation in beef extract-peptone liquid medium with connecing collarium, under 25 ~ 35 DEG C of temperature, 150 ~ 240rpm aerobic condition, concussion is cultivated 1 ~ 3 day to exponential phase, obtains liquid bacterial agent seed liquor; Culture medium prescription is: beef extract: 5g/L, peptone: 10g/L, NaNO₂: 1g/L, wherein adds 0.5mL/L methyl alcohol in Staphylococcus cohnis FSDN-C culture medium.

3, above-mentioned Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1 and Shui Shi Flavobacterium FDN-2 seed liquor are amplified respectively to cultivation according to 3:2:1,1:1:1 and the combination of 1:2:3 ratio in the reactor with good stirring system, nitrite nitrogen concentration in nutrient solution is 200mg/L ~ 800mg/L, carbon-nitrogen mass ratio 2:1 ~ 10:1; Condition of culture is 25～35 DEG C of temperature; PH is 6.5～10.0; Dissolved oxygen is lower than 1.0mg/L.

To cultivating through amplifying the liquid bacterial suspension A (seed liquor mixed proportion 3:2:1), the B(seed liquor mixed proportion 1:1:1 that obtain) and C(seed liquor mixed proportion 1:2:3) collect and concentrate, then add the nutrient solution of bacteria suspension two volumes. NO in every liter of nutrient solution₂ ^-、Fe^2＋、Mg^2＋、K^＋、Ca^2＋These five kinds of cationic mole of allocation ratios are 2000:5:20:20:15, wherein NO₂ ^-Mass concentration be 500mg/L, obtain denitrification microbial inoculum A, denitrification microbial inoculum B and denitrification microbial inoculum C, in denitrification microbial inoculum, the volume of thalline accounts for 30%.

Embodiment 3

Certain enterprise can produce the high ammonia nitrogen containing wastewater from catalyst of a large amount of saliferous in Catalyst Production process, and waste water quality is characterized as ammonia nitrogen concentration 400 ~ 600mg/L, and COD concentration is 200 ~ 500mg/L, and pH is 8.87, electrical conductivity 35 ~ 40ms/cm, Cu²⁺Concentration is 2 ~ 10mg/L, Zn²⁺Concentration is 10 ~ 20mg/L.

Adopt SBR PROCESS FOR TREATMENT, the temperature of controlling system for handling is 30 DEG C, and DO is 2 ~ 4mg/L, and pH value is 7.8. While going into operation first using the activated sludge of oil refining sewerage factory as kind of a mud, after throwing in, MLSS is 4000mg/L, be after vexed quick-fried 7 days, to start to add nitrobacteria under 2 ~ 5mg/L condition at DO, be that 30mg/L adds according to MLSS first, be that 20mg/L adds by MLSS again two days later, in adding nitrobacteria, add the promoter I of embodiment 1 according to promoter concentration 30mg/L in sewage disposal system. Operation two days later, intrasystem nitrite nitrogen accounts for 50% of total nitration product, now add the denitrification microbial inoculum A of embodiment 2, added once every 5 days, add by the volume ratio that accounts for the amount of disposing of sewage 0.05% per hour first, successively successively decrease, each dosage successively decreases 30% than last time, when adding denitrification microbial inoculum A, add promoter I according to promoter concentration 20mg/L in sewage disposal system at every turn later. Move that after 15 days, water outlet ammonia nitrogen concentration is lower than 25mg/L, total nitrogen concentration is lower than 40mg/L, and two kinds of thalline all stop adding. Continue operation a period of time, water outlet ammonia nitrogen concentration lower than 15mg/L, total nitrogen concentration lower than 25mg/L, COD concentration lower than 50mg/L, electrical conductivity is lower than 1500 μ s/cm, Cu²⁺Concentration is lower than 0.5mg/L, Zn²⁺Concentration, lower than 1.0mg/L, has realized the up to standard processing of saliferous containing ammonia sewage.

Embodiment 4

The existing sewage quality of certain catalyst plant is: ammonia nitrogen concentration 50 ~ 300mg/L, COD is 50 ~ 500mg/L, pH8.93, electrical conductivity 25 ~ 35ms/cm, Cu²⁺Concentration is 5 ~ 10mg/L, Zn²⁺Concentration is 15 ~ 25mg/L, also contains the nitrate of 400mg/L left and right simultaneously. Due to the impact of the metal ions such as high salt concentration and copper zinc, cause sewage can not realize processing up to standard. Adopt the inventive method to process, in the time that the MLSS in original system is 3000mg/L, add nitrobacteria, be that 40mg/L adds according to MLSS first, in adding nitrobacteria, add the promoter II of embodiment 1 according to promoter concentration 20mg/L in sewage disposal system, after moving one week, water outlet ammonia nitrogen concentration, lower than 25mg/L, can stop adding nitrobacteria and promoter. Continuing one week rear nitrous rate of operation is 60%, now add the promoter II of embodiment 1 and the denitrification microbial inoculum B of embodiment 2, in adding microbial inoculum at every turn according to sewage disposal system in promoter concentration 20mg/L add promoter, microbial inoculum first dosage is that the volume ratio that accounts for the amount of disposing of sewage 0.01% per hour adds, and adds once, each dosage successively decreases 50% than last time every 3 days. Move that after one month, water outlet ammonia nitrogen and total nitrogen concentration are all lower than 25mg/L, electrical conductivity is lower than 1500 μ s/cm, Cu²⁺Concentration is lower than 0.5mg/L, Zn²⁺Concentration is lower than 1.0mg/L, and in system, sludge character is good, has realized the effective processing of saliferous containing ammonia catalyst sewage.

Embodiment 5

Certain chemical plant saliferous is containing ammonia containing wastewater from catalyst, and waste water quality is characterized as: ammonia nitrogen concentration 50 ~ 100mg/L, COD concentration is 50 ~ 200mg/L, pH8.97, electrical conductivity 15 ~ 25ms/cm, Cu²⁺Concentration is 5mg/L, Zn²⁺Concentration is 10mg/L. Adopt this waste water of SBR PROCESS FOR TREATMENT, due to the impact of the metal ions such as high salt concentration and copper zinc, cause moving year sewage and can not realize all the time processing up to standard. Adopting the inventive method to process, is that 30mg/L adds according to MLSS first, adds the promoter III of embodiment 1 in adding nitrobacteria according to promoter concentration 25mg/L in sewage disposal system. Operation two days later, intrasystem nitrite nitrogen accounts for 50% of total nitration product, now add the promoter III of embodiment 1 and the denitrification microbial inoculum C of embodiment 2, in adding microbial inoculum at every turn according to sewage disposal system in promoter concentration 25mg/L add promoter, microbial inoculum adds by the volume ratio that accounts for the amount of disposing of sewage 0.08% per hour, added once every 5 days, each dosage successively decreases 50% than last time. Move that after 20 days, water outlet ammonia nitrogen concentration is lower than 15mg/L, total nitrogen concentration is lower than 25mg/L, and electrical conductivity is lower than 1500 μ s/cm, Cu²⁺Concentration is lower than 0.5mg/L, Zn²⁺Concentration, lower than 1.0mg/L, has realized the up to standard processing of saliferous containing ammonia sewage.

Embodiment 6

Treatment process and operating condition are with embodiment 3, and difference is: growth of microorganism promoter adopts the promoter IV in embodiment 1. Move that after 15 days, water outlet ammonia nitrogen concentration is lower than 20mg/L, total nitrogen concentration is lower than 30mg/L, and two kinds of thalline all stop adding. Continue operation a period of time, water outlet ammonia nitrogen concentration lower than 10mg/L, total nitrogen concentration lower than 20mg/L, COD concentration lower than 45mg/L, electrical conductivity is lower than 1400 μ s/cm, Cu²⁺Concentration is lower than 0.5mg/L, Zn²⁺Concentration, lower than 1.0mg/L, has realized the up to standard processing of saliferous containing ammonia sewage.

Claims

1. the biological denitrification method of the high saline sewage of a Catalyst Production process discharge, it is characterized in that comprising following content: using sludge sewage as microbe carrier, add nitrobacteria and growth of microorganism promoter at activated sludge concentration under lower than 5000mg/L condition, when accounting for 50% of total nitration product, nitrite nitrogen in system adds denitrification microbial inoculum and growth of microorganism promoter when above, when ammonia nitrogen and total nitrogen concentration are during all lower than 50mg/L, startup stage, finishes, and enters steady running operation; Described growth of microorganism promoter comprises slaine and polyamines, and wherein slaine is 40 ~ 100 weight portions, and polyamines is 5 ~ 30 weight portions; Described slaine is made up of calcium salt, mantoquita, magnesium salts and/or ferrous salt.

2. method according to claim 1, it is characterized in that: be under 2000～4000mg/L condition, to add nitrobacteria and growth of microorganism promoter in sludge concentration, when nitrite nitrogen in system account for total nitration product 50%～80% time add denitrification microbial inoculum and growth of microorganism promoter; In described growth of microorganism promoter, slaine is 50 ~ 80 weight portions, and polyamines is 10 ~ 20 weight portions.

3. method according to claim 1 and 2, is characterized in that: the slaine in described growth of microorganism promoter is calcium salt, magnesium salts and mantoquita, wherein Ca²⁺、Mg²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (0.5 ~ 5); Or calcium salt, ferrous salt and mantoquita, wherein Ca²⁺、Fe²⁺And Cu²⁺Mol ratio be (5 ~ 15): (1 ~ 8): (0.5 ~ 5); Or calcium salt, magnesium salts, ferrous salt and mantoquita, wherein Ca²⁺、Mg²⁺、Fe²⁺And Cu²⁺Mol ratio be (5 ~ 15): (5 ~ 25): (1 ~ 8): (0.5 ~ 5).

4. method according to claim 3, is characterized in that: when described slaine is calcium salt, magnesium salts and mantoquita, and Ca²⁺、Mg²⁺And Cu²⁺Mol ratio be (8 ~ 12): (10 ~ 20): (1 ~ 4); Or when calcium salt, ferrous salt and mantoquita, Ca²⁺、Fe²⁺And Cu²⁺Mol ratio be (8 ~ 12): (2 ~ 6): (1 ~ 4); Or when calcium salt, magnesium salts, ferrous salt and mantoquita, Ca²⁺、Mg²⁺、Fe²⁺And Cu²⁺Mol ratio be (8 ~ 12): (10 ~ 20): (2 ~ 6): (1 ~ 4).

5. according to the method described in claim 1,2 or 3, it is characterized in that: the calcium salt in described growth of microorganism promoter is CaSO₄Or CaCl₂; Magnesium salts is MgSO₄Or MgCl₂; Ferrous salt is FeSO₄Or FeCl₂; Mantoquita is CuSO₄Or CuCl₂, preferably CuSO₄; Polyamines is spermine, spermidine or both mixtures.

6. method according to claim 1 and 2, is characterized in that: described growth of microorganism promoter can also comprise inorganic acid azanol, and content is 0.5 ~ 15 weight portion.

7. method according to claim 6, is characterized in that: described inorganic acid azanol is one or more in hydroxylamine hydrochloride, HAS or phosphatic hydroxylamine, and content is 2 ~ 10 weight portions.

8. method according to claim 1, is characterized in that: the water quality characteristic of described high saline sewage is: ammonia nitrogen concentration is generally 30 ~ 600mg/L, and COD is 30 ~ 1000mg/L, and electrical conductivity is 5 ~ 60ms/cm, Cu²⁺Concentration is 2 ~ 50mg/L, Zn²⁺Concentration is 2 ~ 100mg/L, and SS is 10 ~ 1000mg/L, and pH is 6 ~ 10.

9. method according to claim 1, is characterized in that: sludge sewage is from oil refining sewerage treatment plant, catalyst sewage treatment plant or municipal wastewater treatment plant; The temperature of controlling sewage disposal is 18-40 DEG C, is preferably 25-35 DEG C, and dissolved oxygen is 0.1 ~ 4.0mg/L, is preferably 0.2 ~ 3.0mg/L, and pH is 7.0-9.0, is preferably 7.5-8.5.

10. method according to claim 1, is characterized in that: the nitrobacteria adding comprises Nitromonas and Nitrosomas, and wherein Nitrosomas accounts for the more than 60% of nitrobacteria total quantity.

11. methods according to claim 1, is characterized in that: denitrification microbial inoculum contain Staphylococcus cohnis (Staphylococcuscohnii) FSDN-C, arthrobacterium (Arthrobactercreatinolyticus) FDN-1 and Shui Shi Flavobacterium (Flavobacteriummizutaii) at least one in FDN-2, three kinds of bacterial strains are respectively on July 14th, 2011 and on March 11st, 2010 and be preserved in that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", deposit number is respectively CGMCCNO.5062, CGMCCNo.3657, CGMCCNo.3659.

12. methods according to claim 1, is characterized in that: the dosage of growth of microorganism promoter adds according to promoter concentration 10 ~ 40mg/L in sewage disposal system; Nitrobacteria dosage is that 30 ~ 100mg/L adds according to MLSS, when ammonia nitrogen concentration in waste water stops adding during lower than 50mg/L; Denitrification microbial inoculum divides and adds for 3 ~ 10 times, added once every 1～7 day, add by the volume ratio that accounts for the amount of disposing of sewage 0.001% ~ 0.1% per hour first, successively successively decrease later, each dosage successively decreases 30%～50% than last time, after total nitrogen concentration stops adding during lower than 50mg/L, adds thalline, sewage disposal system can not spoil disposal in 3 months.