CN108118008A - Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application - Google Patents

Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application Download PDF

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CN108118008A
CN108118008A CN201611072924.8A CN201611072924A CN108118008A CN 108118008 A CN108118008 A CN 108118008A CN 201611072924 A CN201611072924 A CN 201611072924A CN 108118008 A CN108118008 A CN 108118008A
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microbial inoculum
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高会杰
郭志华
孙丹凤
赵胜楠
陈明翔
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China Petroleum and Chemical Corp
Sinopec Fushun Research Institute of Petroleum and Petrochemicals
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Sinopec Fushun Research Institute of Petroleum and Petrochemicals
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria

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Abstract

The present invention relates to a kind of salt tolerant high-effective microorganism microbial inoculum, secondary coccus is contained in microbial inoculum（Paracoccus sp.）Microbacterium is seen in FSTB 2, north（Microbacterium kitamiense）FSTB 4, Pseudomonas stutzeri（Pseudomonas stutzeri）At least one of FSTB 5, simultaneously containing marsh cock Salmonella（Kocuria palustris）FSDN A and Staphylococcus cohnis (Staphylococcus cohnii）FSDN C, and contain arthrobacterium（Arthrobacter creatinolyticus）FDN 1 and Shui Shi Flavobacteriums（Flavobacterium mizutaii）At least one of FDN 2.The invention also discloses the preparation method and application of above-mentioned microbial inoculum.The microbial bacterial agent is used directly for total nitrogen and COD in processing high slat-containing wastewater, and can also be added in all kinds of biochemical reaction structures improves microorganism group into the salt resistant character of microorganism system, improves the total nitrogen of system and COD removal efficiencies in optimization wastewater treatment.

Description

Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application
Technical field
The invention belongs to field of environment microorganism, microbial bacterial agent and its preparation side more particularly to a kind of Efficient salt-tolerant Method and application.The microbial inoculum can realize micro- life for high slat-containing wastewater processing with the COD and total nitrogen in efficient removal brine waste Industrialization, the commercialization of object microbial inoculum.
Background technology
From the industries such as oil, chemical industry, medicine and chemical fertilizer salt content higher than 1% high slat-containing wastewater generally contain compared with High organic pollution and nitrogen-containing pollutant, Pollutants in Wastewater, which is constantly discharged into environment, can cause serious pollution problem. The shortage problem getting worse of freshwater resources, the processing and its recycling of waste water are more valued by people.
The physically or chemically COD in method processing high slat-containing wastewater, it is of high cost and be likely to result in secondary pollution.Tradition is raw Object method has great advantage when handling Low-salinity waste water, but in high salt, high soda acid, high temperature or low temperature inverse ring border, microorganism Degradation capability will be suppressed.The problem of Facultative Halophiles technology is based on engineering field generates with being badly in need of, by science side Method tames out the advantage Facultative Halophiles of processing high slat-containing wastewater, which can be with its unique eucaryotic cell structure and material composition It is grown in the environment of higher salinity.Facultative Halophiles processing high slat-containing wastewater has the unrivaled advantage of conventional activated sludge process, And it is more at low cost than physical-chemical process, more apparent economic benefit and social benefit can be generated, can be that related wastewater treatment reaches Mark discharge provides technical support.
In high slat-containing wastewater process field, salt content is a key factor for influencing microbiological treatment waste water efficiency. Believe glad etc.（Biological reinforcing technology processing highly salt containing organic waste water [J] water technologies, 2008,8：66-70）Using biological reinforced Technical finesse highly salt containing organic waste water, using the dehydrogenation of the biological reinforced saponin waste water biological treatment system activated sludge of Facultative Halophiles Enzymatic activity significantly improves, and when system tolerance chlorine ion concentration is up to 2.8%, the removal rate of saponin waste water COD is 84.41%. CN201210130657.0, CN201210130644.3, CN201010536065.X and CN201210130658.5 are related to adopting With marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, denitrogenation pair ball Bacterium DN-3 and Methylobacterium SDN-3 is to ammonia nitrogen, total nitrogen and the COD in brine waste and catalytic cracking catalyst waste water The method for carrying out biological treatment.This method processing COD relies primarily on denitrification removing nitrite and realizes that processing capacity is limited, Application range is also somewhat limited.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of high-effective microorganism microbial inoculum of salt tolerant and preparation method thereof.It should Microbial bacterial agent is used directly for total nitrogen and COD in processing high slat-containing wastewater, can also be added to all kinds of biochemical reaction structures Building improves microorganism group into the salt resistant character of microorganism system, the total nitrogen and COD for improving system take off in optimization wastewater treatment in object Except rate.
Salt tolerant high-effective microorganism microbial inoculum of the present invention contains secondary coccus（Paracoccus sp.）Microbacterium is seen in FSTB-2, north （Microbacterium kitamiense）FSTB-4, Pseudomonas stutzeri（Pseudomonas stutzeri）In FSTB-5 At least one, simultaneously containing marsh cock Salmonella（Kocuria palustris）FSDN-A and Staphylococcus cohnis (Staphylococcus cohnii）FSDN-C, and contain arthrobacterium（Arthrobacter creatinolyticus）FDN-1 With Shui Shi Flavobacteriums（Flavobacterium mizutaii）At least one of FDN-2, wherein secondary coccus FSTB-2, north are shown in Microbacterium FSTB-4, Pseudomonas stutzeri FSTB-5 were preserved in " Chinese microorganism strain preservation pipe on June 1st, 2015 Reason committee common micro-organisms center ", deposit number are respectively CGMCC No.10938, CGMCC No.10939, CGMCC No.10940, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica.Secondary coccus FSTB-2 has applied for disclosure in CN201510737219.4, and has submitted preservation and survival proof；Microbacterium FSTB-4 is seen in north Disclosure is applied in CN201510737150.5, and has had submitted preservation and survival to prove；Pseudomonas stutzeri FSTB-5 exists Disclosure is applied in CN201510737176.X, and has had submitted preservation and survival to prove.Wherein marsh cock Salmonella FSDN-A, section Family name staphylococcus FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, in CN103103141A, CN103014128A, Disclosed in CN102465106A, CN102465105A, deposit number be respectively CGMCC NO.5061, CGMCC NO.5062, CGMCC No.3657、CGMCC No.3659。
In microbial bacterial agent of the present invention, " secondary coccus FSTB-2, north are shown in microbacterium FSTB-4, Pseudomonas stutzeri FSTB-5 At least one of ", " marsh cock Salmonella FSDN-A ", " Staphylococcus cohnis FSDN-C ", " arthrobacterium FDN-1 and Shui Shi is yellow The volume ratio of four bacteroids of at least one of bacillus FDN-2 " is 1-5:1:1:0.1-10.（By thalline stereometer, thalline volume To centrifuge obtained thalline volume after five minutes after culture under the conditions of 10,000 turns per minute, similarly hereinafter）.It is preferred that contain simultaneously Secondary coccus FSTB-2, Pseudomonas stutzeri FSTB-5, marsh cock Salmonella FSDN-A and Staphylococcus cohnis FSDN-C.
Further, in the microbial inoculum containing Paracoccus denitrificans (Paracoccus denitrificans) DN-3 and first Base shaft bacterium (Methylobacterium phyllosphaerae) at least one of SDN-3, wherein Paracoccus denitrificans DN-3 and For Methylobacterium SDN-3 disclosed in CN102465104A and CN102465103, deposit number is respectively CGMCC No.3658 and CGMCC No.3660, addition are to be with the volume ratio of above-mentioned 4 bacteroid：1-5:1:1:0.1-10:0.1-10.
In microbial bacterial agent of the present invention, secondary coccus FSTB-2 colony colours are ecru, and bacterial strain individual is spherical, gram It dyes as feminine gender, oxidase positive, catalase is negative, and decomposable asymmetric choice net utilizes several kinds of carbon source, has nitrate reduction activity.North is seen micro- Bacillus FSTB-4 colony colours are shallower taupe brown, and bacterial strain is individual for rod-shaped, Gram-positive, oxidase negative, catalase The positive, decomposable asymmetric choice net utilize several kinds of carbon source, have nitrate reduction characteristic.Pseudomonas stutzeri FSTB-5 colony colours are shallow turmeric Color, bacterial strain individual are rod-shaped, Gram-negative, and oxidase negative contacts enzyme positive, has nitrate reduction performance, decomposable asymmetric choice net Utilize several kinds of carbon source.
In microbial bacterial agent of the present invention, microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 are seen in secondary coccus FSTB-2, north The efficient removal of COD in the brine waste of salt content 1.0wt%-5.0wt% can be applied individually to any.
In microbial bacterial agent of the present invention, suitable additive, such as nutriment, preservation auxiliary agent can be contained, specifically Additive types and dosage are well known to those skilled in the art.
The present invention also provides the preparation method of above-mentioned salt tolerant high-effective microorganism microbial inoculum, including herein below：
（1）Secondary coccus FSTB-2, north are seen that microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 are inoculated in FSTB solids respectively It is activated on culture medium；By marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Huang bar Bacterium FDN-2, Paracoccus denitrificans DN-3, Methylobacterium SDN-3 are inoculated on corresponding solid medium and are activated respectively；
（2）See microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 bacterium with make even secondary coccus FSTB-2 on plate, north of collarium is connect Fall and be inoculated in respectively in corresponding FSTB liquid mediums, made even marsh cock Salmonella FSDN-A on plate, Coriolis with collarium is connect Staphylococcus FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 bacterium colonies It is inoculated in respectively in corresponding liquid medium, 24-72 hours is cultivated under the conditions of 20-40 DEG C of temperature, 150-240rpm to right Number growth period obtains liquid bacterial agent seed liquor；
（3）Thalline will be collected after the amplification culture of above-mentioned seed liquor, mixed in required ratio, be that salt tolerant of the present invention is high Imitate microbial bacterial agent.
Microbacterium FSTB-4 and Pseudomonas stutzeri are seen in secondary coccus FSTB-2 involved by microbial bacterial agent of the present invention, north The thalline of FSTB-5 is activated is with FSTB Liquid Culture based formulas used in seed liquor culture：FeSO4•7H2O 25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/ L, pH value 6.0-8.0.Involved marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3, the activation of Methylobacterium SDN-3 thalline and culture medium used in seed liquor culture are CN103103141A, CN103014128A, CN102465106A, CN102465105A, CN102465104A and Culture medium disclosed in CN102465103.Solid medium is that 2% agar is added in aforesaid liquid culture medium.
In the present invention, secondary coccus FSTB-2, north are shown in the kind of tri- kinds of bacterium of microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 Sub- liquid is amplified culture after can individually amplifying culture or the seed liquor mixing of three kinds of bacterium.Marsh cock Salmonella The seed liquor of FSDN-A and Staphylococcus cohnis FSDN-C need individually amplification culture；Arthrobacterium FDN-1 and Shui Shi Flavobacterium The seed liquor of FDN-2 can individually amplify culture or the common amplification culture of the seed liquor mixing of two kinds of bacterium；Denitrogenation pair ball The seed liquor of bacterium DN-3 and Methylobacterium SDN-3 can individually amplify culture or the seed liquor mixing of two kinds of bacterium is common Amplification culture.
The culture solution that the amplification culture of bacterial strain involved by microbial bacterial agent of the present invention is used can be that saliferous is nitrogenous containing COD Fluid nutrient medium or saliferous is containing actual waste water nitrogenous COD, wherein salt content 0.5wt%-10wt%, COD（Cr methods, under Together）For 200-20000mg/L, total nitrogen 50-1000mg/L.The condition of culture be 20-40 DEG C of temperature, pH 6.0-8.0, Dissolved oxygen is 0.1-3.0mg/L.Amplification cultivation reactor used in the present invention can have good aeration and stirring system Structures.
The preparation process of microbial bacterial agent of the present invention carries out thalline activation first, then carries out seed liquor culture, finally will Thalline is collected after seed liquor amplification culture, packs and saves backup.Above-mentioned amplification cultivates to obtain the higher dense bacterium solution of concentration can be with Use is added directly, can be prepared after can also these dense bacterium solutions being added in nutrient solution or nutrient solution and protectant mixed liquor Into liquid microbial inoculum, the existing method in this field can also be utilized to be prepared into the microbial inoculum of dry powder-shaped, as the case may be in room temperature or It is preserved under person's cryogenic conditions.
Salt tolerant high-effective microorganism microbial inoculum prepared by the present invention can be applied to the processing of various high slat-containing wastewaters, the salt of tolerance Content is 1wt%-10wt, and COD removal rates are up to more than 90%, and nitrogen removal rate is up to more than 95%.The microbial inoculum can be added directly useless It in water treatment system, can also be mixed with activated sludge as inoculum, biofilm can also post-process waste water on various fillers, The efficient removal of COD and total nitrogen suitable for high slat-containing wastewater, the saliferous higher especially suitable for nitrate nitrogen and nitrous nitrogen content give up Water.
Microbial bacterial agent of the present invention is shown in microbacterium FSTB-4, Pseudomonas stutzeri FSTB- by secondary coccus FSTB-2, north 5th, marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, denitrogenation pair ball A variety of bacterial strain compositions such as bacterium DN-3 and Methylobacterium SDN-3.In high-salt wastewater processing procedure, these types of microorganism mutually matches It closes, ammonia nitrogen, total nitrogen and COD removal rates are higher than effect when any microorganism is used alone, Salt-tolerant microbial agent pair The tolerance impact capacity of waste water is stronger than single bacterial strain, has widened the scope of application of waste water quality, improves Determination of Total Nitrogen in Waste Water and COD Treatment effect.
Mainly microorganism is prepared the microbial bacterial agent of the present invention similar in growth conditions, each bacterial strain in mixed liquor Ratio from the influence of amplification factor, be conducive to fast culture.The microbial inoculum is used directly for handling COD in high slat-containing wastewater And total nitrogen, the composition for improving original microorganism in traditional waste water structures can be also added to, strengthens Biochemical Treatment.
When for handling high-salt wastewater, some bacterial strains can be secreted viscous the microbial bacterial agent of the present invention by hypersaline environment stimulation Property substance, the flocculating property of sludge can be improved, beneficial bacterium is reduced and be lost in；The removal of calcium ions and magnesium ions is also helped, can reduce and remove The hard pharmaceutical use of calcium reduces the reagent cost during Halite water reuse.
Specific embodiment
The present invention proposes a kind of use in waste water treatment salt tolerant high-effective microorganism microbial inoculum and preparation method thereof.Microbial bacterial agent is grown Speed is fast, and collecting amount is big, and microbial inoculum has stronger salt-tolerant trait and adaptability, has preferable impact resistance；It can directly throw It is added in waste water treatment plant's activated sludge and uses, total nitrogen containing high concentration and COD can also be handled in suitable biochemical reactor Waste water.System start-up time can be shortened after coming into operation, widen the scope of application of waste water quality, realize the micro- life of wastewater treatment Industrialization, the commercialization of object microbial inoculum.
In the present invention, secondary coccus FSTB-2, north are shown in the FSTB used in microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 Liquid Culture based formulas is：FeSO4•7H2O 25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value 6.0-8.0；FSTB solid mediums are trained in liquid Support the agar that 20g/L is added in base.
The thalline of marsh cock Salmonella FSDN-A is activated in the present invention is with the culture medium prescription used in seed liquor culture：Ox Meat extract:10g/L, peptone:15g/L, NaNO2:1.0g/L, KNO3:0.8g/L.The thalline of Staphylococcus cohnis FSDN-C is lived Change and be with the culture medium prescription used in seed liquor culture：Beef extract:10g/L, peptone:15g/L, NaNO2:1.0g/L, first Alcohol:1.0mL/L.Culture medium prescription used in the thalline activation and seed liquor culture of arthrobacterium FDN-1 and Shui Shi Flavobacterium FDN-2 For：Beef extract:5g/L, peptone:10g/L, NaNO2:1g/L, solid medium add in 1.5 ~ 2.5% agar.Denitrogenation pair ball The Liquid Culture based formulas of bacterium DN-3 is：KNO3:1g/L, sodium succinate:8g/L, KH2PO4:1g/L, FeCL2:0.5g/L.Methyl The Liquid Culture based formulas of bacillus SDN-3 is：Ammonium sulfate:0.5g/L, methanol:0.75mL/L, KH2PO4:1g/L, FeCL2: 0.5g/L.Above-mentioned solid medium needs to add in 20g/L agar.
The preparation method of 1 microbial bacterial agent of embodiment
（1）Secondary coccus FSTB-2, north are seen that microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 are inoculated in FSTB solids respectively It is activated on culture medium；By marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Huang bar Bacterium FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 are inoculated on corresponding solid medium and are activated respectively；Coating Temperature is placed on after uniformly to be activated in 35 DEG C of constant incubators.
（2）See microbacterium FSTB-4 and Pseudomonas stutzeri FSTB- with make even secondary coccus FSTB-2 on plate, north of collarium is connect 5 bacterium colonies are inoculated in respectively in corresponding FSTB fluid nutrient mediums, are made even marsh cock Salmonella FSDN-A on plate, section with collarium is connect Family name staphylococcus FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 bacterium Fall and be inoculated in respectively in corresponding fluid nutrient medium, shake culture 48 hours to logarithm is given birth under the conditions of 30 DEG C of temperature, 150rpm For a long time, liquid bacterial agent seed liquor is obtained.
（3）Microbacterium is shown in secondary coccus FSTB-2, north respectively in the reactor with good aeration and stirring condition FSTB-4, Pseudomonas stutzeri FSTB-5, marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacteriums FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 seed liquors are amplified culture, and COD is dense in culture solution It spends for 2000mg/L, total nitrogen concentration 100mg/L, salt content 2.5wt%, pH value 8.0.Condition of culture is：Temperature is 30 DEG C, dissolved oxygen 2.0-3.0mg/L when incubation time 72 is small, is derived from the dense bacterium solution of nine plants of bacterium.
The dense bacterium solution obtained by amplifying culture is collected, and is prepared according to the ratio described in table 1, specifically such as Shown in table 1.
The composition and ratio of 1 Salt-tolerant microbial agent of table
2 microbial bacterial agent of embodiment handles brine waste
The high slat-containing wastewater that certain chemical enterprise generates, salt content 2.5wt%, COD 2500mg/L, ammonia nitrogen 20mg/L, total nitrogen For 150mg/L, Ca2+Concentration 700mg/L, Mg2+Concentration 500mg/L.Occur COD in During Process of Long-term Operation and total nitrogen removal efficiency is bright Aobvious variation situation, water outlet are unsatisfactory for emission request.The micro- life of salt tolerant for adding the preparation of embodiment 1 by the 1% of wastewater treatment volume daily Object microbial inoculum is launched 15 days altogether, and treatment effect is as shown in table 2.
The treatment effect of the different salt tolerant microbial inoculums of table 2
As shown in Table 2, after the combination microbial inoculum prepared using the present invention is handled, effluent COD concentration is consistently lower than 60mg/L, and water outlet is total Nitrogen concentration is consistently lower than 40mg/L, while Ca2+And Mg2+Removal rate is all higher than 20%, and salt content also reduces 19%.And similary Under the conditions of be higher than using the microbial inoculum or effluent COD concentration of non-invention combination higher than 100mg/L or total nitrogen concentration 100mg/L, it is impossible to realize that total nitrogen and COD meet emission request simultaneously, compared with microbial inoculum of the present invention, Ca2+And Mg2+And salt content It is not substantially reduced.It can be seen that microbial bacterial agent of the present invention can improve the treatment effect of brine waste, brine waste is realized Advanced treating, while the salt content of waste water and calcium ions and magnesium ions concentration can be reduced.

Claims (13)

1. a kind of microbial bacterial agent of Efficient salt-tolerant, it is characterised in that contain secondary coccus（Paracoccus sp.）FSTB-2, north See microbacterium（Microbacterium kitamiense）FSTB-4, Pseudomonas stutzeri（Pseudomonas stutzeri） At least one of FSTB-5, simultaneously containing marsh cock Salmonella（Kocuria palustris）FSDN-A and Coriolis grape ball Bacterium (Staphylococcus cohnii）FSDN-C, and contain arthrobacterium（Arthrobacter creatinolyticus） FDN-1 and Shui Shi Flavobacteriums（Flavobacterium mizutaii）At least one of FDN-2, wherein secondary coccus FSTB-2, North sees that microbacterium FSTB-4, Pseudomonas stutzeri FSTB-5 were preserved in " Chinese microorganism strain guarantor on June 1st, 2015 Hide administration committee's common micro-organisms center ", deposit number is respectively CGMCC No.10938, CGMCC No.10939, CGMCC No.10940；Wherein marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN- 2 deposit number is respectively CGMCC NO.5061, CGMCC NO.5062, CGMCC No.3657, CGMCC No.3659.
2. microbial inoculum according to claim 1, it is characterised in that：In the microbial inoculum, " microbacterium is seen in secondary coccus FSTB-2, north At least one of FSTB-4, Pseudomonas stutzeri FSTB-5 ", " marsh cock Salmonella FSDN-A ", " Staphylococcus cohnis FSDN-C ", " at least one of arthrobacterium FDN-1 and Shui Shi Flavobacteriums FDN-2 ", the volume ratio of four bacteroids is 1-5:1:1: 0.1-10。
3. microbial inoculum according to claim 1 or 2, it is characterised in that：Contain secondary coccus FSTB-2 in the microbial inoculum simultaneously, apply Family name pseudomonad FSTB-5, marsh cock Salmonella FSDN-A and Staphylococcus cohnis FSDN-C.
4. microbial inoculum according to claim 1 or 2, it is characterised in that：Contain Paracoccus denitrificans in the microbial inoculum (Paracoccus denitrificans) DN-3 and Methylobacterium (Methylobacterium phyllosphaerae) At least one of SDN-3, deposit number are respectively CGMCC No.3658 and CGMCC No.3660, and addition is and above-mentioned 4 The volume ratio of bacteroid is：1-5:1:1:0.1-10:0.1-10.
5. microbial inoculum according to claim 1, it is characterised in that：It is helped in the microbial inoculum containing suitable nutriment and preservation Agent.
6. the preparation method of any salt tolerant high-effective microorganism microbial inoculums of claim 1-4, it is characterised in that including herein below：
（1）Secondary coccus FSTB-2, north are seen that microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 are inoculated in FSTB solids respectively It is activated on culture medium；By marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, arthrobacterium FDN-1, Shui Shi Huang bar Bacterium FDN-2, Paracoccus denitrificans DN-3, Methylobacterium SDN-3 are inoculated on corresponding solid medium and are activated respectively；
（2）See microbacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 bacterium colonies with make even secondary coccus FSTB-2, north on plate of collarium is connect It is inoculated in corresponding FSTB liquid mediums, is made even marsh cock Salmonella FSDN-A, Coriolis grape on plate with collarium is connect respectively Coccus FSDN-C, arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 bacterium colonies difference It is inoculated in respective liquid culture solution, 24-72 hours is cultivated under 20-40 DEG C of temperature, 150-240rpm to exponential phase, is obtained Obtain liquid bacterial agent seed liquor；
（3）Thalline will be collected after the amplification culture of above-mentioned seed liquor, mixed in required ratio, be that salt tolerant of the present invention is high Imitate microbial bacterial agent.
7. according to the method described in claim 6, it is characterized in that：Microbacterium is seen in secondary coccus FSTB-2 involved by microbial inoculum, north The thalline of FSTB-4 and Pseudomonas stutzeri FSTB-5 are activated is with FSTB Liquid Culture based formulas used in seed liquor culture： FeSO4•7H2O 25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef Cream 5g/L, peptone 10g/L, pH value 6.0-8.0；Involved marsh cock Salmonella FSDN-A, Staphylococcus cohnis FSDN-C, Arthrobacterium FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3, the activation of Methylobacterium SDN-3 thalline and seed liquor culture Used culture medium for CN103103141A, CN103014128A, CN102465106A, CN102465105A, Culture medium disclosed in CN102465104A and CN102465103；Solid medium is to add in 2% in aforesaid liquid culture medium Agar.
8. according to the method described in claim 6, it is characterized in that：Microbacterium FSTB-4 and Amur are seen in secondary coccus FSTB-2, north The seed liquor of tri- kinds of bacterium of pseudomonad FSTB-5 is amplified training after individually amplifying culture or the seed liquor mixing of three kinds of bacterium It supports；The seed liquor of marsh cock Salmonella FSDN-A and Staphylococcus cohnis FSDN-C need individually amplification culture；Arthrobacterium FDN-1 Individually amplify culture or the common amplification culture of the seed liquor mixing of two kinds of bacterium with the seed liquor of Shui Shi Flavobacteriums FDN-2；It is de- The seed liquor of nitrogen pair coccus DN-3 and Methylobacterium SDN-3 individually amplify culture or the seed liquor mixing of two kinds of bacterium is common Amplification culture.
9. according to the method described in claim 6, it is characterized in that：Culture solution used is saliferous containing Liquid Culture nitrogenous COD Base or saliferous are containing actual waste water nitrogenous COD, wherein salt content 0.5wt%-10wt%, COD 200-20000mg/L, Total nitrogen is 50-1000mg/L.
10. according to the method described in claim 6, it is characterized in that：The condition of culture is 20-40 DEG C of temperature, and pH is 6.0-8.0, dissolved oxygen 0.1-3.0mg/L.
11. according to the method described in claim 6, it is characterized in that：Amplification culture obtains the higher dense bacterium solution of concentration can be straight It connects and adds use, can be prepared into after can also these dense bacterium solutions being added in nutrient solution or nutrient solution and protectant mixed liquor Liquid microbial inoculum can also utilize the existing method in this field to be prepared into the microbial inoculum of dry powder-shaped, be protected under room temperature or cryogenic conditions It deposits.
12. the application of microbial bacterial agent described in claim 1, it is characterised in that：The Salt-tolerant microbial agent can be applied to respectively The processing of kind of high slat-containing wastewater, the salt content of tolerance are 1wt%-10wt, and COD removal rates are up to more than 90%, and nitrogen removal rate is up to 95% More than.
13. the application of microbial bacterial agent described in claim 12, it is characterised in that：The microbial inoculum can be added directly wastewater treatment It in system, can also be mixed with activated sludge as inoculum, biofilm can also post-process waste water on various fillers, be suitable for Nitrate nitrogen and the higher brine waste of nitrous nitrogen content.
CN201611072924.8A 2016-11-29 2016-11-29 Microbial bacterial agent of Efficient salt-tolerant and its preparation method and application Pending CN108118008A (en)

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