CN103374524A - Salt-tolerant microbial agent and preparation method thereof - Google Patents
Salt-tolerant microbial agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.
Description
Technical field
The invention belongs to the environmental microorganism field, relate in particular to a kind of wastewater treatment microbial microbial inoculum and preparation method thereof, this microbial inoculum can be finished removing of ammonia nitrogen, total nitrogen and CODcr, realizes being used for industrialization, the commercialization of wastewater treatment microbial microbial inoculum.
Background technology
Biological denitrificaion is to solve one of comparatively cost-effective method of nitrate pollution.No matter be traditional microorganic adhesion type wastewater treatment structures or high-performance bio film processing system newly developed, if be responsible for the microbial host Autotrophic nitrification bacterium of denitrogenation.The rate of propagation of autotrophic bacteria self is slow, can't compete with heterotrophic organism in the Sludge System of mixed culture, be difficult to obtain higher biomass, nitrification efficiency is low, causes that autotrophic microorganism denitrification system impact resistance is weak, nitrification is incomplete, nitrogen removal rate is low.So some denitrification microorganisms novel, better effects if are found in succession such as allotrophic nitrobacteria, aerobic denitrifying bacteria, anaerobic ammonium oxidizing bacteria etc.
Anaerobic ammonium oxidizing bacteria belongs to obligatory anaerobic bacteria, although be applied to have less energy-consumption in short distance nitration and the Anammox combination process, hang down sludge yield, hang down the characteristics such as cost of investment and working cost, it is too high that but the employing two-stage process is prone to the nitrite concentration of short distance nitration stage accumulation, and then the problem that microorganisms is suppressed, in different reactors, carry out respectively with Anammox owing to short distance nitration simultaneously, so that the investment of the capital cost of whole technique and floor space increase.If adopt one-level technique then owing to use the anaerobic ammonium oxidizing bacteria of obligate anaerobic to be in for a long time in the certain density aerobic environment, thereby reduced to a certain extent the activity of anaerobic ammonium oxidizing bacteria.So up to the present, short distance nitration-anaerobic ammoxidation technique engineering is in the world used and mainly contained everywhere: the sludge digestion liquid of Rotterdam, NED Dokhaven municipal wastewater treatment plant is processed engineering, Dutch Lichtenvoorde leather-making waste water is processed project, Mie Prefecture, Japan semiconductor machining wastewater treatment project and Dutch Olburgen potato processing waste water and processed project, does not also have large-scale promotion application.
Allotrophic nitrobacteria fast growth, cell yield be high, require dissolved oxygen concentration low, also strong to the adaptive faculty of environment, compare with the autotrophic type nitrifier, although the speed ratio autotrophic bacteria of the heterotrophic bacterium oxidation ammonium salt of unit biomass is slow, the speed of the oxidation ammonium salt that it is overall is slow unlike autotrophic bacteria.Some heterotrophic microorganism can lack under the condition of organic carbon source, the oxidation of carrying out ammonia obtains the required energy of growth, also can carry out ammonia oxidation under the condition that organism exists, and does not obtain energy, be a kind of metabolic process, the oxidation of ammonia is not subjected to organic restriction.Therefore allotrophic nitrobacteria receives much attention as a kind of novel denitrification microorganism.The investigator conducts extensive research at aspects such as heterotrophic nitrification microbe to screen, functional metabolism approach, enzyme and genes both at home and abroad, but still only rest at present the laboratory study stage, the example that really nitrification bacteria is applied to processing waste water in the Practical Project is actually rare.The aerobic denitrifying bacteria particularly research of nitrite type aerobic denitrifying bacteria has remedied the deficiency of short distance nitration-anaerobic ammoxidation technique, can directly use the short-cut nitrification and denitrification denitrogenation that the combination of heterotrophic nitrification and aerobic denitrifying bacteria realizes one-level technique.Therefore the dominant strain that growth conditions is close makes up and is prepared into microbial inoculum, the biological restoration that is added in the conventional Waste Water Treatment or is used for specific polluted-water can improve nitric efficiency, improve water quality, promote commercialization, the business-like process of denitrification microorganism microbial inoculum, also will be conducive to the further industrial application of the novel denitrification process such as short-cut nitrification and denitrification, synchronous nitration and denitrification.
CN101302485A discloses a kind of heterotrophic nitrification microbial preparation, its cultural method and purposes, this microbial inoculum contain germ oligotrophy unit cell (
Stenotrophomonas maltophiliastrainDN 1.1) and pseudomonas putida (
Pseudomonas putida strainDN 1.2), ammonia nitrogen and the total nitrogen of this microbial inoculum in can the effective elimination water body can also be removed the COD in the organic waste water simultaneously, is applicable to high-concentration culture waste water and processes.This microbial inoculum is when processing ammonia nitrogen concentration and be the piggery wastewater of 455 ~ 600mg/L, and experiment moves to 94 ~ 95h, and the clearance of ammonia nitrogen in the waste water is reached 87% ~ 88%, and processing the water outlet ammonia-nitrogen content is 59 ~ 72mg/L; The total nitrogen of water inlet 790mg/L can be processed to 164mg/L after processing 95h, nitrogen removal rate is 79.2%.CN200910021020.7 discloses a kind of preparation method who falls the water quality modifying microecological preparation of ammonia nitrogen and nitrous acid nitrogen; relate to a strain Arthrobacter CGMCC No.1282 in this microbial inoculum, but the probiotics of this invention belongs to aquaculture technology and ecological environmental protection technical field.
Have following two problems in the existing short-cut nitrification and denitrification technique: (1) adopts two-stage process too high and to the microorganisms restraining effect at the nitrite concentration of short distance nitration stage accumulation; (2) adopt one-level technique because long-term aerobic environment can reduce the anaerobic ammonium oxidizing bacteria activity to a certain extent.
Summary of the invention
For the problem that prior art exists, the invention provides and a kind ofly can adopt microbiobacterial agent that one-level technique carries out denitrogenation and preparation method thereof.This microbiobacterial agent is except the problem that solves existing short-cut nitrification and denitrification existence, can be added to and improve the composition of the microorganism, raising nitric efficiency in all kinds of biochemical reaction structures, simultaneously the nitration reaction process can be effectively controlled, the organic pollutant in the waste water can also be effectively removed.
Salt-durable microbe microbial inoculum of the present invention contain Staphylococcus cohnis (
Staphylococcus cohnii) FSDN-C,
Arthrobacter creatinolyticusFDN-1 and
Flavobacterium mizutaiiAmong the FDN-2 one or both, contain simultaneously Paracoccus denitrificans (
Paracoccus denitrificans) DN-3 and
Methylobacterium phyllosphaeraeAmong the SDN-3 one or both, five kinds of bacterial strains are respectively at being preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcode 100101) on July 14th, 2011 and on March 11st, 2010.Staphylococcus cohnis (
Staphylococcus cohnii) the FSDN-C deposit number is CGMCC NO.5062,
Arthrobacter creatinolyticusThe FDN-1 deposit number is CGMCC No.3657,
Flavobacterium mizutaiiThe FDN-2 deposit number is CGMCC No.3659, Paracoccus denitrificans (
Paracoccus denitrificans) the DN-3 deposit number be CGMCC No.3658,
Methylobacterium phyllosphaeraeThe SDN-3 deposit number is CGMCC No.3660.
Wherein: Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 can be wherein a kind of, also can be the mixing thalline of two kinds of arbitrary proportions; Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 can be wherein a kind of, also can be the mixing thalline of two kinds of arbitrary proportions." Staphylococcus cohnis FSDN-C, " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " are 1:0.1 ~ 10:0.1～10 with the ratio of " Paracoccus denitrificans DN-3 and/or methyl bacillus SDN-3 " three bacteroids, are preferably 1:0.2 ~ 5:0.2～5.(press the thalline volumeter, the thalline volume turns the thalline volume that obtain of centrifugation after 5 minutes under the condition for cultivating afterwards at per minute 10,000, and is lower same).
In the microbiobacterial agent of the present invention, Staphylococcus cohnis FSDN-C colony colour is white, and the bacterial strain individuality is spherical, and without gemma, gramstaining is positive, and catalase is positive, and oxidase negative can utilize several kinds of carbon source.Arthrobacter FDN-1 colony colour is yellow, bacterial strain is individual is bar-shaped, without gemma, can move; Gramstaining is positive, and catalase is positive, and oxidase negative can utilize several kinds of carbon source.Shui Shi Flavobacterium FDN-2 colony colour is white, bacterial strain is individual is shaft-like, without gemma; Gramstaining is negative, and catalase is positive, and oxidase positive can be utilized several kinds of carbon source.Paracoccus denitrificans DN-3 bacterium colony is milk yellow; The individual ovalize of bacterial strain; Gramstaining is negative, and catalase is positive, oxidase positive; Can utilize cheap carbon source.Methyl bacillus SDN-3 bacterium colony is orange, is Gram-negative bacteria, and thalline is shaft-like, can move; Circular tiny, neat in edge is smooth; Catalase is positive, and oxidase positive can be utilized several kinds of carbon source.
In the microbiobacterial agent of the present invention, can also contain marsh cock Salmonella (
Kocuria palustris) FSDN-A, marsh cock Salmonella FSDN-A is preserved on July 14th, 2011 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center "; depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcode 100101), deposit number is CGMCC NO.5061.Marsh cock Salmonella FSDN-A accounts for the 10%-30% of total thalline volume in microbiobacterial agent.
Staphylococcus cohnis FSDN-C in the microbial inoculum of the present invention, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 all can finish denitrification process under aerobic and anaerobic environment.Wherein Staphylococcus cohnis FSDN-C can carry out denitrification denitrogenation take nitrite nitrogen as nitrogenous source, can remove COD in denitrogenation; Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 can carry out denitrification denitrogenation take nitrite nitrogen as nitrogenous source, can remove COD in denitrogenation; Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 all can carry out the heterotrophic nitrification-aerobic denitrification simultaneous denitrification as nitrogenous source with ammonia nitrogen, can remove COD in denitrogenation.
In the microbiobacterial agent of the present invention, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2 can mix in any proportion but must comprise wherein a kind ofly, and Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 also can mix according to arbitrary proportion but must comprise wherein a kind of.In the microbiobacterial agent of the present invention, can contain suitable additive, such as nutritive substance, preservation auxiliary agent etc., concrete additive types and consumption are well known to those skilled in the art.
A kind of concrete preparation method of wastewater treatment microbial microbial inoculum of the present invention comprises following content:
1, Staphylococcus cohnis FSDN-C of the present invention, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2, the secondary coccus DN-3 of nitrogen and methyl bacillus SDN-3 are inoculated on the solid medium respectively activate;
2, be inoculated in respectively in the corresponding liquid medium with connecing the bacterium colony that collarium makes even on the plate, concussion is cultivated 1 ~ 3 day to logarithmic phase under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition, obtains the liquid bacterial agent seed liquor;
3, will collect thalline after the above-mentioned seed liquor amplification culture, mix in required ratio after the filtering and concentrating, add the required required additives such as nutritive medium, be microbiobacterial agent of the present invention.The seed liquor of every kind of bacterium is amplification culture separately, also can the FDN-1 seed liquor and the FDN-2 seed liquor be mixed in proportion common amplification culture, DN-3 seed liquor and SDN-3 seed liquor are mixed in proportion common amplification culture, also can be that amplification culture is carried out in the seed liquor mixing of four kinds of bacterium.
The thalline activation of the Staphylococcus cohnis FSDN-C that microbiobacterial agent of the present invention is related and seed liquor are cultivated used culture medium prescription and are: extractum carnis: 5 ~ 10g/L, peptone: 8 ~ 15g/L, NaNO
2: 0.4 ~ 1.0g/L, methyl alcohol: 0.25 ~ 1.0mL/L.The thalline activation of the Arthrobacter FDN-1 that microbiobacterial agent of the present invention is related and Shui Shi Flavobacterium FDN-2 and seed liquor are cultivated used culture medium prescription and are: extractum carnis: 3 ~ 7g/L, peptone: 7 ~ 13g/L, NaNO
2: 0.8 ~ 1.5g/L, solid medium adds 1.5 ~ 2.5% agar.The thalline activation of the Paracoccus denitrificans DN-3 that microbiobacterial agent of the present invention is related and methyl bacillus SDN-3 and seed liquor are cultivated used culture medium prescription and are: ammonium sulfate: 0.1 ~ 0.5g/L, KNO
3: 0.5 ~ 1.0g/L, Soduxin 2 ~ 8g/L, methyl alcohol: 0.25 ~ 1.0mL/L, solid medium adds 2% agar; Also contain in addition a small amount of iron ion and magnesium ion etc. in the nutrient solution.Culture condition is: temperature is that 20 ~ 35 ℃, 150 ~ 240rpm concussion are cultured to logarithmic phase and can gather in the crops thalline for the preparation of microbiobacterial agent.
The used nutrient solution of microbiobacterial agent amplification culture of the present invention can be autogamy or actual waste water, and the total nitrogen in the nutrient solution and ammonia nitrogen concentration are 100mg/L ~ 1000mg/L, carbon-nitrogen mass ratio 5:1 ~ 15:1; Culture condition is 15 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is lower than 3.0mg/L.The used amplification culture reactor of the present invention can be various suitable structures, has good aeration and stirring system.The pH value does not need to regulate in the culturing process.
Obtain the higher bacteria suspension of concentration behind the concentration of above-mentioned amplification culture; with being prepared into liquid microbial inoculum after these bacteria suspensions adding nutritive mediums or nutritive medium and the protectant mixed solution, also can utilize the existing method in this area to be prepared into the microbial inoculum of dry powder.Can adopt anti-storage, container or the equipment antifreeze, that be convenient to materials such as transporting such as plastics bag, Plastic Bottle or plastic tank to pack.Packaging Method adopts the existing routine techniques in this area, and Packing Unit is that the microbial inoculum after 50 ~ 5000g/ bag (bag or bottle) packing can be preserved under room temperature or cold condition as the case may be.The room temperature preservation quality guaranteed period is 1 ~ 6 month, and the cryopreservation quality guaranteed period is 1 ~ 5 year, and the activity decreased rate is less than 10% after preserving.
The used concentration method of the present invention can not affect by centrifugal or filtration etc. the method for microbial activity.
Microbial inoculum preparation process of the present invention is at first carried out the thalline activation; Then carry out seed liquor and cultivate, will collect thalline after the seed liquor amplification culture, concentrate, pack and save backup at last.The microbiobacterial agent tolerance and the strong adaptability that obtain, good impact resistance, the removal load of total nitrogen is high, treatment effect is good; Can directly be added to mix in the Waste Water Treatment or with active sludge or on various fillers biofilm aftertreatment waste water, use properties is good; Microbiobacterial agent resume speed through the certain hour preservation is fast, and biological activity is high, can realize industrialization, the commercialization of denitrification microorganism.
Microbiobacterial agent of the present invention by Staphylococcus cohnis FSDN-C, Arthrobacter (
Arthrobacter creatinolyticus) FDN-1, Shui Shi Flavobacterium (
Flavobacterium mizutaii) FDN-2, Paracoccus denitrificans (
Paracoccus denitrificans) DN-3 and methyl bacillus (
Methylobacterium phyllosphaerae) SDN-3 bacterial strain composition.In the nitrogenous effluent treatment process, particularly adopt one-level technique to carry out in the biological denitrificaion treating processes, substrate is worked in coordination, competed mutually to these several microorganisms, the effect that ammonia nitrogen and TN removel rate are higher than any microorganism when using separately, because so population effect is strong, wide to the scope of application of waste water quality to the thalline of the withstand shock energy force rate purifying of waste water, can process and contain higher ammonia nitrogen and COD waste water, the denitrogenation of real realization short-cut nitrification and denitrification obviously improves water treatment effect.
Wastewater treatment microbial microbial inoculum of the present invention mainly is prepared from by the close microorganism of growth conditions, wherein culture condition and the growth velocity of Staphylococcus cohnis FSDN-C, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are close, culture condition and the growth velocity of Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 are close, so the ratio of each bacterial strain is not subjected to the impact of magnification in the mixed solution, is conducive to fast culture.This microbial inoculum can be added to composition, the reinforcement Biochemical Treatment that improves original microorganism in traditional waste water structures on the one hand, when newly-built wastewater treatment structures, can save denitrogenation structures series on the other hand, denitrification reaction is directly occurred in the one-level biological tank, reduce initial cost, reduced working cost.Staphylococcus cohnis FSDN-C can utilize several kinds of carbon source, have certain salt resistant character, can be applicable in the treating processes of high slat-containing wastewater, and this bacterial strain can be secreted the flocculence that a kind of material strengthens mud under environmental stimulus simultaneously.This has further widened the range of application of microbiobacterial agent.
Embodiment
The present invention proposes a kind of use in waste water treatment microbiobacterial agent and preparation method thereof.The liquid microbial inoculum fast growth of the present invention, collecting amount is large, and microbial inoculum has stronger tolerance and adaptability, has preferably shock resistance; Can directly be added in waste water treatment plant's active sludge and use, also can in suitable biochemical reactor, process the waste water that contains ammonia nitrogen in high density and COD.Can shorten system start time after coming into operation, widen the scope of application of waste water quality, can realize industrialization, the commercialization of wastewater treatment microbial microbial inoculum.
The preparation method of embodiment 1 microbiobacterial agent
1, thalline activation: the thalline activation of Staphylococcus cohnis FSDN-C and seed liquor are cultivated used culture medium prescription and are: extractum carnis: 6g/L, peptone: 8g/L, NaNO
2: 0.6g/L, methyl alcohol: 0.5mL/L; The activation culture based formulas of Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 is: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L, the agar of adding 2.0%; The activation culture based formulas of Paracoccus denitrificans DN-3 is: KNO
3: 1g/L, Soduxin: 8g/L, KH
2PO
4: 1g/L, FeCL
2: 0.5g/L, the agar of adding 2.0%; The activation culture based formulas of methyl bacillus SDN-3 is: ammonium sulfate: 0.5g/L, methyl alcohol: 0.75mL/L, KH
2PO
4: 1g/L, FeCL
2: 0.5g/L; Add 2% agar.Being placed on temperature after flat board coating evenly is to activate in 30 ℃ of constant incubators.
2, be inoculated in respectively in the corresponding liquid medium with the thalline that connects on the collarium scraping flat board, concussion is cultivated 1 ~ 3 day to logarithmic phase under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition, obtains the liquid bacterial agent seed liquor; Culture medium prescription need not add agar with the activation culture based formulas.
3, with above-mentioned Staphylococcus cohnis FSDN-C as the I seed liquor, above-mentioned Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are mixed as II seed liquor (thalline is numbered respectively II-1 and II-2 according to 1:1 and two kinds of ratio combinations of 0.5:1), above-mentioned Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 are mixed as III seed liquor (thalline is numbered respectively III-1 and III-2 according to 1:1 and two kinds of ratio combinations of 0.5:1), the I seed liquor, in in the II seed liquor arbitrary group or any microorganism and the III seed liquor arbitrary group or any microorganism mix according to 1:1:1 and two kinds of ratios of 1:2:3 carry out respectively amplification culture in having the reactor of good stirring system, ammonia nitrogen concentration in the nutrient solution is 200mg/L ~ 800mg/L, carbon-nitrogen mass ratio 5:1 ~ 10:1; Culture condition is 25 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is lower than 3.0mg/L.
To liquid bacterial suspension A (seed liquor I, seed liquor II-1 and seed liquor III-1 blending ratio 1:1:1), B(seed liquor I, seed liquor II-2 and the seed liquor III-2 blending ratio 1:2:3 that obtains through amplification culture), C(seed liquor I, seed liquor II-1 and seed liquor III-2 blending ratio 1:1:1), D(seed liquor I, Arthrobacter FDN-1 and seed liquor III-2 blending ratio 1:2:3), E(seed liquor I, seed liquor II-2 and Paracoccus denitrificans DN-3 blending ratio 1:1:3) collect, concentrated, then add the nutritive medium of bacteria suspension two volumes.NH in every liter of nutritive medium
4 +-N, Fe
2+, Mg
2+, K
+, Ca
2+These five kinds cationic mole allocation ratios are 2000:5:20:20:15, add behind the preserving agent of bacteria suspension volume 2.5% minute to install in the Plastic Bottle of 500ml again, put under-70 ℃ of conditions to save backup.
Embodiment 2 microbiobacterial agents are processed saliferous and are contained the ammonia nitrogen containing wastewater from catalyst
Certain chemical plant saliferous contains ammonia nitrogen containing wastewater from catalyst water quality characteristic: pH8.91, and specific conductivity is 5.8mS/cm, and COD is 530mg/L, ammonia nitrogen 237mg/L.In system's During Process of Long-term Operation, the ammonia nitrogen removal weak effect causes total nitrogen concentration up to 182mg/L, and the SVI of active sludge is greater than 200 mL/g, and settling property is relatively poor.Every day is according to the 1%(volume ratio) in system, add a certain amount of liquid bacterial suspension A, threw in altogether 10 days.SVI in the detection system moves month rear water outlet ammonia nitrogen of post analysis processing and is lower than 15mg/L less than 200mL/g, and total nitrogen is lower than 25mg/L.This shows that this microbiobacterial agent can improve the treatment effect of saliferous ammonia-containing water, can improve the settling property of mud simultaneously.
Claims (11)
1. salt-durable microbe microbial inoculum is characterized in that: microbiobacterial agent contain Staphylococcus cohnis (
Staphylococcus cohnii) FSDN-C, Arthrobacter (
Arthrobacter creatinolyticus) FDN-1 and Shui Shi Flavobacterium (
Flavobacterium mizutaii) among the FDN-2 one or both, contain simultaneously Paracoccus denitrificans (
Paracoccus denitrificans) DN-3 and methyl bacillus (
Methylobacterium phyllosphaerae) among the SDN-3 one or both, " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", deposit number are respectively CGMCC NO.5062, CGMCC No.3657, CGMCC No.3659, CGMCC No.3658, CGMCC No.3660 to five kinds of bacterial strains respectively at being preserved on July 14th, 2011 and on March 11st, 2010.
2. microbiobacterial agent according to claim 1 is characterized in that: Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are wherein a kind of, or the mixing thalline of two kinds of arbitrary proportions; Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 are wherein a kind of, or the mixing thalline of two kinds of arbitrary proportions.
3. microbiobacterial agent according to claim 1 and 2 is characterized in that: " Staphylococcus cohnis FSDN-C ", " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " are 1:0.1 ~ 10:0.1～10 with the volume ratio of " Paracoccus denitrificans DN-3 and/or methyl bacillus SDN-3 " three bacteroids.
4. microbiobacterial agent according to claim 1 and 2 is characterized in that: " Staphylococcus cohnis FSDN-C ", " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " are 1:0.2 ~ 5:0.2～5 with the volume ratio of " Paracoccus denitrificans DN-3 and/or methyl bacillus SDN-3 " three bacteroids.
5. microbiobacterial agent according to claim 1 is characterized in that: the Staphylococcus cohnis colony colour is white, and the bacterial strain individuality is spherical, and without gemma, gramstaining is positive, and catalase is positive, and oxidase negative can utilize several kinds of carbon source; Arthrobacter FDN-1 colony colour is yellow, bacterial strain is individual is bar-shaped, without gemma, can move, and gramstaining is positive, and catalase is positive, and oxidase negative can utilize several kinds of carbon source; Shui Shi Flavobacterium FDN-2 colony colour is white, bacterial strain is individual is shaft-like, without gemma; Gramstaining is negative, and catalase is positive, and oxidase positive can be utilized several kinds of carbon source; Paracoccus denitrificans DN-3 bacterium colony is milk yellow, the individual ovalize of bacterial strain, and gramstaining is negative, and catalase is positive, and oxidase positive can be utilized cheap carbon source; Methyl bacillus SDN-3 bacterium colony is orange, is Gram-negative bacteria, and thalline is shaft-like, can move, and circular tiny, neat in edge is smooth, and catalase is positive, and oxidase positive can be utilized several kinds of carbon source.
6. microbiobacterial agent according to claim 1, it is characterized in that: Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 all can finish denitrification process under aerobic and anaerobic environment; Wherein Staphylococcus cohnis FSDN-C can carry out denitrification denitrogenation take nitrite nitrogen as nitrogenous source, can remove COD in denitrogenation; Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 can carry out denitrification denitrogenation take nitrite nitrogen as nitrogenous source, can remove COD in denitrogenation; Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 all can carry out the heterotrophic nitrification-aerobic denitrification simultaneous denitrification as nitrogenous source with ammonia nitrogen, can remove COD in denitrogenation.
7. microbiobacterial agent according to claim 1 is characterized in that: contain nutritive substance, preservation auxiliary agent in the microbiobacterial agent.
8. microbiobacterial agent according to claim 1 is characterized in that: contain in the microbiobacterial agent marsh cock Salmonella (
Kocuria palustris) FSDN-A, marsh cock Salmonella FSDN-A is preserved on July 14th, 2011 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center "; deposit number is CGMCC NO.5061, marsh cock Salmonella FSDN-A accounts for the 10%-30% of total thalline volume in microbiobacterial agent.
9. the preparation method of the described microbiobacterial agent of the arbitrary claim of claim 1 to 8 comprises following content:
Staphylococcus cohnis FSDN-C, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and methyl bacillus SDN-3 are inoculated on the solid medium respectively and activate;
Be inoculated in respectively in the corresponding liquid medium with connecing the bacterium colony that collarium makes even on the plate, cultivate and obtain the liquid bacterial agent seed liquor;
To collect thalline after the above-mentioned seed liquor amplification culture, mix in required ratio after the filtering and concentrating, add required nutritive medium, be the salt-durable microbe microbial inoculum.
10. method according to claim 9 is characterized in that: the cultural method of step (2) liquid bacterial agent seed liquor obtains the liquid bacterial agent seed liquor for to cultivate 1 ~ 3 day to logarithmic phase under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition.
11. method according to claim 9 is characterized in that: the independent amplification culture of seed liquor of every kind of bacterium in the step (3); Perhaps FDN-1 seed liquor and FDN-2 seed liquor are mixed in proportion common amplification culture, and DN-3 seed liquor and SDN-3 seed liquor are mixed in proportion common amplification culture; Or amplification culture is carried out in the seed liquor of five kinds of bacterium mixing.
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Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103540555A (en) * | 2013-11-05 | 2014-01-29 | 中蓝连海设计研究院 | Method for preparing salt-tolerant microbial agent |
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| CN103540555A (en) * | 2013-11-05 | 2014-01-29 | 中蓝连海设计研究院 | Method for preparing salt-tolerant microbial agent |
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| CN108048361A (en) * | 2017-12-28 | 2018-05-18 | 中国农业大学 | Staphylococcus cohnis S154 and its application in biosynthesis nanometer selenium |
| CN108704938A (en) * | 2018-04-28 | 2018-10-26 | 江苏世邦生物工程科技有限公司 | Complex micro organism fungicide and preparation method thereof and the application in soil remediation with high salt |
| CN108840446A (en) * | 2018-06-28 | 2018-11-20 | 绍兴市海清环保科技有限公司 | A kind of mix bacterium agent for high salinity Industrial Waste Water Treatments |
| CN109402193A (en) * | 2018-11-08 | 2019-03-01 | 浙江海洋大学 | A method of flocculant is prepared using ocean cock Salmonella |
| CN111377544A (en) * | 2018-12-29 | 2020-07-07 | 中蓝连海设计研究院有限公司 | High-salinity wastewater treatment process utilizing halophilic bacteria YL5-2 |
| CN111377544B (en) * | 2018-12-29 | 2021-12-31 | 中蓝连海设计研究院有限公司 | High-salinity wastewater treatment process utilizing halophilic bacteria YL5-2 |
| CN112940995A (en) * | 2021-04-28 | 2021-06-11 | 武汉大学 | Arthrobacter capable of degrading n-eicosane and application thereof |
| CN113292159A (en) * | 2021-05-28 | 2021-08-24 | 复旦大学 | Enhanced CFBR (circulating fluidized bed reactor) process applied to high ammonia nitrogen-containing wig wastewater treatment |
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