CN103373767A - Method for biologically denitrifying high-salinity sewage generated in production process of catalysts - Google Patents
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Abstract
The invention discloses a method for biologically denitrifying high-salinity sewage generated in the production process of catalysts. The method is implemented by adding nitrobacteria and denitrifiers with nitrites as electron acceptors to sewage, wherein the high-salinity sewage treatment temperature is 18-40 DEG C; the dissolved oxygen is 0.2-3mg/L; and the pH value is 7.5-8.5. The method adopts autotrophic bacteria and heterotrophic bacteria which are subjected to combination of superiority to serve as reinforced microorganisms for treating sewage, adopts different addition modes, can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and truly achieves biological denitrification of the high-salinity sewage generated in the production process of catalysts by being combined with high-efficiency devices.
Description
Technical field
The invention belongs to environmental engineering dirty water living creature processing technique field, be specifically related to a kind of high biological denitrification method that contains salt catalyst sewage.
Background technology
Ammonium salt and ammoniacal liquor are used in many places in the Catalyst Production process of petrochemical industry, therefore contain a large amount of ammonia nitrogens in the containing wastewater from catalyst of discharging.Although the ammonia nitrogen in the waste water can adopt the physico-chemical processes such as stripping stripping, ion-exchange, chemical oxidation to process, there are the problems such as by product secondary pollution and processing efficiency be low in these methods.By contrast, biological process is the better method of control water body ammonia and nitrogen pollution.
The traditional biological method is as the terminal technology of conventional sewage disposal, and is limited in one's ability when processing high ammonia nitrogen, low COD waste water, usually sacrifices load in the hope of realizing the qualified discharge of ammonia nitrogen pollutant in the waste water.Particularly when salt concn is higher in the sewage, can suppress microbial growth and reduce microbic activity, bring certain difficulty to biological treatment.Although all carried out repeatedly a large amount of improvement from aspects such as technique and sewage treatment structures, in sewage treatment process, play certain effect, but do not change owing to be responsible for the main body active sludge of ammonia nitrogen removal, so the removal effect of ammonia nitrogen still is not very desirable.The ammonia nitrogen problem that exceeds standard directly has influence on the comprehensive up to standard and normal production of Catalyst Production enterprise wastewater, becomes the bottleneck of restriction enterprise development, Ammonia Wastewater Treatment is become the primary environmental issue of Catalyst Production enterprise.Therefore the treatment technology of ammonia nitrogen becomes current study hotspot in the salt catalyst wastewater to research and develop economic, practical, safe containing, to protection of the environment, promote the well-being of mankind significant.
No matter be traditional or novel bio-denitrification technology, increasing biomass is one of effective means that improves sewage treating efficiency.Along with some novel, effective denitrification microorganisms, separated evaluation is out in succession such as allotrophic nitrobacteria, aerobic denitrifying bacteria, anaerobic ammonium oxidizing bacteria etc., biological reinforcing technology is widely applied, and people have carried out a large amount of research work to bacteria agent.
CN101302485A discloses a kind of heterotrophic nitrification microbial preparation, its cultural method and purposes, this microbial inoculum contain germ oligotrophy unit cell (
Stenotrophomonas maltophiliastrainDN 1.1) and pseudomonas putida (
Pseudomonas putida strainDN 1.2), ammonia nitrogen and the total nitrogen of this microbial inoculum in can the effective elimination water body can also be removed the COD in the organic waste water simultaneously, is applicable to high-concentration culture waste water and processes.This microbial inoculum is when processing ammonia nitrogen concentration and be the piggery wastewater of 455 ~ 600mg/L, and experiment moves to 94 ~ 95h, and the clearance of ammonia nitrogen in the waste water is reached 87% ~ 88%, and processing the water outlet ammonia-nitrogen content is 59 ~ 72mg/L; The total nitrogen of water inlet 790mg/L can be processed to 164mg/L after processing 95h, nitrogen removal rate is 79.2%.CN200910021020.7 discloses a kind of preparation method who falls the water quality modifying microecological preparation of ammonia nitrogen and nitrous acid nitrogen; relate to a strain Arthrobacter CGMCC No.1282 in this microbial inoculum, but the probiotics of this invention belongs to aquaculture technology and ecological environmental protection technical field.Result of use is limited in the denitrogenation processing of the high saline sewage that the mentioned microorganism microbial inoculum produces in the Catalyst Production process, need to be for suitable denitrogenation microbial inoculum and the method for wastewater treatment of high saliferous Catalyst Production sewage development.
Summary of the invention
Although more biotechnological formulation is arranged on the market, all be not suitable for processing the saliferous ammonia-containing water that produces in the Catalyst Production process.For the deficiencies in the prior art, the invention discloses a kind of bioremediation of Catalyst Production saliferous ammonia-containing water, the method is as biological body with the nitrobacteria denitrification microorganism microbial inoculum close with growth conditions, make up rear salinity, ammonia nitrogen and a small amount of metal ion that reduces in the sewage with the Sewage Plant active sludge as microbe carrier, solve the saliferous ammonia-containing water that produces in the existing Catalyst Production process and process problem, make the sewage after the processing can qualified discharge.
Height of the present invention contains in the biological denitrification method of salt catalyst sewage, and sewage quality is characterized as: general 30 ~ 600 mg/L of ammonia nitrogen concentration, the COD(Cr method, as follows) concentration is 30 ~ 1000mg/L, specific conductivity 5 ~ 60ms/cm also contains the Cu of 2 ~ 50mg/L
2+, 2 ~ 100mg/L Zn
2+, the SS(suspended substance) and be 10 ~ 1000mg/L, pH6 ~ 10.The unloading phase operating process as follows: the temperature of sewage disposal is 18-40 ℃, and dissolved oxygen is 0.1 ~ 4 mg/L, is preferably 0.2 ~ 3mg/L, and pH is 7.0-9.0, is preferably 7.5-8.5; With sludge sewage as microbe carrier, being lower than under the 4000mg/L condition preferred activated sludge concentration at activated sludge concentration is to add nitrobacteria under 1000～3000mg/L condition, when nitrite nitrogen in the system account for total nitration product be preferably 50%～80% more than 50% the time add the denitrification microbial inoculum, when ammonia nitrogen and nitrate all are lower than 50 mg/L and preferably are lower than 25mg/L, unloading phase, finish, and enters the steady running operation.The denitrification microbial inoculum that the inventive method is used contain Staphylococcus cohnis (
Staphylococcus cohnii) FSDN-C, and
Arthrobacter creatinolyticusFDN-1 and
Flavobacterium mizutaiiAt least a among the FDN-2.Staphylococcus cohnis (
Staphylococcus cohnii) FSDN-C is preserved on July 14th, 2011 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address are No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcodes 100101), and deposit number is CGMCC NO.5062.
Arthrobacter creatinolyticusFDN-1 is preserved on March 11st, 2010 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address are No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcodes 100101), and deposit number is CGMCC NO.3657.
Flavobacterium mizutaiiFDN-2 is preserved on March 11st, 2010 that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address are No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcodes 100101), and deposit number is CGMCC NO.3659.
In the inventive method, sludge sewage can be from oil refining sewerage treatment plant or catalyzer sewage work or municipal wastewater treatment plant etc.
In the inventive method, the nitrobacteria that adds comprises Nitromonas and Nitrosomas, be preferably the nitrosification dominant microflora, account for the nitrobacteria more than 60% of nitrifier total quantity such as Nitrosomas, guarantee that the nitrite nitrogen of reaction system accounts for more than 50% of total nitration product.Nitrobacteria can adopt various existing schemes to cultivate, and also can adopt commercially available microbial inoculum, such as the nitrosification dominant microflora that can obtain according to CN201010221166.8.
The inventive method is at first thrown in the active sludge of sewage work in the sewage treatment structure to newly-started system, and the active sludge volumetric usage is for to add by the preferred 4000～10000mg/L of 3000～15000mg/L.Add nitrobacteria behind the vexed suitable time that exposes to the sun, DO is controlled at 3 ~ 5mg/L in the vexed process of exposing to the sun, general vexed exposing to the sun 24 ~ 168 hours.For the system that is moving, can be by sludge concentration in the regulation system to being lower than 5000mg/L, be preferably under the condition of 3000～5000 mg/L and add nitrobacteria.
In the inventive method, the nitrobacteria dosage is that 100 ~ 1000mg/L adds according to MLSS.After adding nitrobacteria, when accounting for 50% of total nitration product, nitrite nitrogen in the system can add the denitrification microbial inoculum when above.
Nitrobacteria can disposablely add also and can add in batches, can stop to add when ammonia nitrogen concentration is lower than 50mg/L in the waste water.The denitrification microbial inoculum need add in batches, preferably divides to add for 3 ~ 10 times, adds once every 1～7 day.Add by the volume ratio that per hour accounts for the amount of disposing of sewage 0.01% ~ 1% first, successively decrease one by one later on, each dosage successively decreases 30%～70% than the last time, when nitrate is lower than 25mg/L, stop to add.Two kinds of thalline also can intersect and add, add thalline after Sewage treatment systems can not spoil disposal in 3 months.For periodical operation technique, per hour the amount of disposing of sewage is the average treatment amount.
In the denitrification microbial inoculum that the inventive method is used, Staphylococcus cohnis FSDN-C colony colour is white, and the bacterial strain individuality is spherical, and without gemma, gramstaining is positive, and catalase is positive, and oxidase negative can utilize several kinds of carbon source.Arthrobacter FDN-1 colony colour is yellow, bacterial strain is individual is bar-shaped, without gemma, can move; Gramstaining is positive, and catalase is positive, and oxidase negative can utilize several kinds of carbon source.Shui Shi Flavobacterium FDN-2 colony colour is white, bacterial strain is individual is shaft-like, without gemma; Gramstaining is negative, and catalase is positive, and oxidase positive can be utilized several kinds of carbon source.
In the denitrification microbial inoculum that the inventive method is used, Staphylococcus cohnis FSDN-C, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 all can carry out denitrification denitrogenation take nitrite nitrogen as nitrogenous source under aerobic and anaerobic environment, can remove COD in denitrogenation.Wherein Staphylococcus cohnis FSDN-C can utilize several kinds of carbon source, has certain salt resistant character, can process high saline sewage.
In the denitrification microbial inoculum that the inventive method is used, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 can be wherein a kind of, also can be the mixing thalline of two kinds of arbitrary proportions, the thalline volume ratio of preferred Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 be 1:0.1 ~ 3." Staphylococcus cohnis FSDN-C " is 1:0.1 ~ 10 with the ratio of " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " two bacteroids, is preferably 1:0.2 ~ 5.(press the thalline volumeter, the thalline volume turns the thalline volume that centrifugation obtained after 5 minutes under the condition for cultivating afterwards at per minute 10,000, and is lower same).In the denitrification microbial inoculum of the present invention, can contain suitable additive, such as nutritive substance, preservation auxiliary agent etc., concrete additive types and consumption are well known to those skilled in the art.The volume sum of above-mentioned thalline accounts for 10%～60% of denitrification microbial inoculum cumulative volume in the denitrification microbial inoculum, is preferably 20%～50%.
A kind of concrete preparation method of the microorganism denitrification microbial inoculum that the inventive method is used comprises following content:
1, Staphylococcus cohnis FSDN-C of the present invention, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are inoculated on the solid medium respectively activate;
2, scrape a ring bacterium liquid and be inoculated in the beef extract-peptone liquid medium with connecing collarium, concussion is cultivated 1 ~ 3 day to logarithmic phase under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition, obtains the liquid bacterial agent seed liquor;
3, thalline, the concentrated microbial inoculum that is the inventive method use will be collected after the above-mentioned seed liquor amplification culture.
The used nutrient solution of microbiobacterial agent amplification culture of the present invention can be autogamy or the actual nitrite nitrogen waste water that contains, and the nitrite nitrogen concentration in the nutrient solution is 100mg/L ~ 1000mg/L, carbon-nitrogen mass ratio 2:1 ~ 10:1; Culture condition is 15 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is lower than 1.0mg/L.
The used amplification culture reactor of the present invention can be various suitable structures, has good aeration and stirring system.The pH value does not need to regulate in the culturing process.
In the inventive method; the microbiobacterial agent that uses is liquid microbial inoculum or solid-state microbial inoculum; obtain the higher bacteria suspension of concentration behind the concentration of above-mentioned amplification culture; to can be prepared into liquid microbial inoculum after these bacteria suspensions adding nutritive mediums or nutritive medium and the protectant mixed solution; liquid microbial inoculum is generally 10～20g/L(MLSS in the concentration of MLSS and represents suspended sediment concentration), also can utilize the existing method in this area to be prepared into the microbial inoculum of dry powder.Can adopt anti-storage, container or the equipment antifreeze, that be convenient to materials such as transporting such as plastics bag, Plastic Bottle or plastic tank to pack.Packaging Method adopts the existing routine techniques in this area, and Packing Unit is that the microbial inoculum after 50 ~ 5000g/ bag (bag or bottle) packing can be preserved under room temperature or cold condition as the case may be.The room temperature preservation quality guaranteed period is 1 ~ 6 month, and the cryopreservation quality guaranteed period is 1 ~ 5 year, and the activity decreased rate is less than 10% after preserving.
The used concentration method of the present invention can not affect by centrifugal or filtration etc. the method for microbial activity.
Microbial inoculum preparation process of the present invention is at first carried out the thalline activation; Then carry out seed liquor and cultivate, will collect thalline after the seed liquor amplification culture, concentrate, pack and save backup at last.The microbiobacterial agent tolerance and the strong adaptability that obtain, good impact resistance, the removal load of total nitrogen is high, treatment effect is good.
The catalyzer saliferous that the present invention proposes contains the treatment process of ammonia sewage, mainly is to realize by the optimum combination that directly adds microorganism in nitrobacteria and denitrification microbial inoculum, the reinforcement sludge, the group status that changes denitrification microorganism.Substrate is worked in coordination, competed mutually to described different microorganism, the effect that ammonia nitrogen and TN removel rate are higher than any microorganism when using separately, particularly replenish can utilize the Staphylococcus cohnis FSDN-C of several kinds of carbon source after, strengthen the salt resistant character of Sewage treatment systems, improved adsorptivity and the flocculence of mud.Because so population effect is strong, wide to the scope of application of waste water quality to the withstand shock ability of waste water, when removing the pollutents such as ammonia nitrogen and COD, be conducive to the removal of salinity and metal ion, and can effectively reduce the turbidity of water outlet, obviously improve the treatment effect of sewage.
Embodiment
The nitrobacteria that embodiment uses is cultivated the nitrosification dominant microflora that obtains by CN201010221166.8 embodiment 1 method.Further specify by the following examples process and the effect of the inventive method.
The preparation of embodiment 1 denitrification microbial inoculum
1, Staphylococcus cohnis FSDN-C of the present invention, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 being inoculated in respectively on the beef extract-peptone solid medium in temperature is to activate in 30 ℃ of constant incubators, culture medium prescription is: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L, the agar of adding 2.0% wherein adds 0.5mL/L methyl alcohol in the Staphylococcus cohnis FSDN-C solid medium.The culture condition of bacterial strain is for being that 30 ~ 35 ℃, 150 ~ 240rpm concussion are cultured to logarithmic phase and can gather in the crops thalline for the preparation of microbiobacterial agent in temperature.
2, scrape a ring solid colony inoculation in the beef extract-peptone liquid medium with connecing collarium, concussion is cultivated 1 ~ 3 day to logarithmic phase under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition, obtains the liquid bacterial agent seed liquor; Culture medium prescription is: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L wherein adds 0.5mL/L methyl alcohol in the Staphylococcus cohnis FSDN-C substratum.
3, above-mentioned Staphylococcus cohnis FSDN-C, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 seed liquor are carried out respectively amplification culture according to 3:2:1,1:1:1 and the combination of 1:2:3 ratio in having the reactor of good stirring system, nitrite nitrogen concentration in the nutrient solution is 200mg/L ~ 800mg/L, carbon-nitrogen mass ratio 2:1 ~ 10:1; Culture condition is 25 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is lower than 1.0mg/L.
To liquid bacterial suspension A (seed liquor blending ratio 3:2:1), the B(seed liquor blending ratio 1:1:1 that obtains through amplification culture) and C(seed liquor blending ratio 1:2:3) collect concentratedly, then add the nutritive medium of bacteria suspension two volumes.NO in every liter of nutritive medium
2 -, Fe
2+, Mg
2+, K
+, Ca
2+These five kinds cationic mole allocation ratios are 2000:5:20:20:15, wherein NO
2 -Mass concentration be 500mg/L, obtain denitrification microbial inoculum A, denitrification microbial inoculum B and denitrification microbial inoculum C, in the denitrification microbial inoculum, the volume of thalline accounts for 30%.
Embodiment 2
The high ammonia nitrogen containing wastewater from catalyst of certain chemical plant saliferous, waste water quality is characterized as ammonia nitrogen concentration 400 ~ 600 mg/L, and COD concentration is 200 ~ 500mg/L, pH8.87, specific conductivity 35 ~ 40ms/cm, Cu
2+Concentration is that 2 ~ 10mg/L, concentration are Zn
2+10 ~ 20mg/L.Adopt the SBR art breading, when going into operation at first with the active sludge of oil refining sewerage factory as kind of a mud, MLSS is 3800mg/L after throwing in, and is to begin to add nitrobacteria after vexed quick-fried 7 days under 2 ~ 5mg/L condition at DO, is that 600mg/L adds according to MLSS first, be that 200mg/L adds by MLSS again two days later, move two days later, DO is 2 ~ 4mg/L, and the pH value is 7.8, intrasystem nitrite nitrogen accounts for 50% of total nitration product, adds the denitrification microbial inoculum A of embodiment 1 this moment.Added once every 5 days, add by the volume ratio that per hour accounts for the amount of disposing of sewage 0.1% first, successively decrease one by one later on, each dosage successively decreases 30% than the last time, move that the water outlet ammonia nitrogen concentration is lower than 25mg/L after 20 days, nitrate is lower than 20mg/L, and two kinds of thalline all stop to add.Continue operation for some time, water outlet ammonia nitrogen and total nitrogen concentration are all stable to be lower than 15mg/L, and COD concentration is lower than 50mg/L, and specific conductivity is lower than 1500 μ s/cm, Cu
2+Concentration is lower than 0.5mg/L, Zn
2+Concentration is lower than 1.0mg/L, has realized that saliferous contains the processing up to standard of ammonia sewage.
Embodiment 3
The existing sewage quality of certain catalyst plant is: ammonia nitrogen concentration 50 ~ 300 mg/L, COD concentration is 50 ~ 500mg/L, pH8.93, specific conductivity 25 ~ 35ms/cm, Cu
2+Concentration is 5 ~ 10mg/L, Zn
2+Concentration is 15 ~ 25mg/L, also contains simultaneously the nitrate about 400mg/L.Because the cation recognitions such as high salt concentration and copper zinc cause sewage can not realize processing up to standard.Adopting the inventive method to process, when the MLSS in the original system is 4000mg/L, add nitrobacteria, is that 600mg/L adds according to MLSS first, and the water outlet ammonia nitrogen concentration is lower than 25mg/L after one week of operation, can stop to add nitrobacteria.Continue that the nitrous rate is 60% after one week of operation, beginning this moment according to dosage first is the denitrification microbial inoculum B that the volume ratio that per hour accounts for the amount of disposing of sewage 0.5% adds embodiment 1, adds once every 3 days, each dosage successively decreases 50% than the last time.Move that water outlet ammonia nitrogen and total nitrogen concentration all are lower than 25mg/L after one month, sludge character is good in the system, has realized that saliferous contains effective processing of ammonia catalyzer sewage.
Embodiment 4
Certain chemical plant saliferous contains the ammonia containing wastewater from catalyst, and waste water quality is characterized as ammonia nitrogen concentration 50 ~ 100 mg/L, and COD concentration is 50 ~ 200mg/L, pH8.97, specific conductivity 15 ~ 25ms/cm, Cu
2+Concentration is 5mg/L, Zn
2+Concentration is 10mg/L.Adopt this waste water of SBR art breading, because the cation recognitions such as high salt concentration and copper zinc cause moving year sewage and can not realize processing up to standard all the time.Adopting the inventive method to process, is that 300mg/L adds according to MLSS first, moves two days later, and DO is 2 ~ 4mg/L, and the pH value is 7.8, and intrasystem nitrite nitrogen accounts for 50% of total nitration product, adds the denitrification microbial inoculum C of embodiment 1 this moment.Add by the volume ratio that per hour accounts for the amount of disposing of sewage 0.8%5 first, added once every 5 days, each dosage successively decreases 50% than the last time.Move that the water outlet ammonia nitrogen concentration is lower than 15mg/L after 20 days, nitrate is lower than 15mg/L, and specific conductivity is lower than 1500 μ s/cm, Cu
2+Concentration is lower than 0.5mg/L, Zn
2+Concentration is lower than 1.0mg/L, has realized that saliferous contains the processing up to standard of ammonia sewage.
Claims (13)
1. one kind high biological denitrification method that contains salt catalyst sewage, the unloading phase operating process temperature be 18-40 ℃, dissolved oxygen is 0.1 ~ 4 mg/L, pH is 7.0-9.0; With sludge sewage as microbe carrier, be lower than under the 4000mg/L condition at activated sludge concentration and add nitrobacteria, add the denitrification microbial inoculum more than 50% when what nitrite nitrogen in the system accounted for total nitration product, when ammonia nitrogen and nitrate all are lower than 50 mg/L, unloading phase, finish, and enters the steady running operation; The denitrification microbial inoculum that uses contain Staphylococcus cohnis (
Staphylococcus cohnii) FSDN-C, and Arthrobacter (
Arthrobacter creatinolyticus) FDN-1 and Shui Shi Flavobacterium (
Flavobacterium mizutaii) at least a among the FDN-2, " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", deposit number are respectively CGMCC NO.5062, CGMCC NO.3657 and CGMCC NO.3659 to three kinds of bacterial strains respectively at being preserved on July 14th, 2011 and on March 11st, 2010.
2. method according to claim 1, it is characterized in that: sludge sewage is from oil refining sewerage treatment plant or catalyzer sewage work or municipal wastewater treatment plant.
3. method according to claim 1 is characterized in that: ammonia nitrogen concentration 30 ~ 600 mg/L of sewage, and COD concentration is 30 ~ 1000mg/L, specific conductivity 5 ~ 60ms/cm contains the Cu of 2 ~ 50mg/L
2+, 2 ~ 100mg/L Zn
2+, suspended substance is 10 ~ 1000mg/L, pH6 ~ 10.
4. method according to claim 1 is characterized in that: the unloading phase operating process dissolved oxygen be 0.2 ~ 3mg/L, pH is 7.5-8.5.
5. method according to claim 1 is characterized in that: be to add nitrobacteria under 1000～3000mg/L condition at activated sludge concentration.
6. method according to claim 1 is characterized in that: when nitrite nitrogen in the system account for total nitration product 50%～80% the time add the denitrification microbial inoculum.
7. method according to claim 1, it is characterized in that: the nitrobacteria that adds comprises Nitromonas and Nitrosomas, and Nitrosomas accounts for the nitrobacteria more than 60% of nitrifier total quantity, and the nitrite nitrogen of reaction system accounts for more than 50% of total nitration product.
8. method according to claim 1, it is characterized in that: the nitrobacteria dosage is that 100 ~ 1000mg/L adds according to MLSS.
9. method according to claim 1, it is characterized in that: nitrobacteria is disposable to be added, and perhaps adds in batches, stops to add when ammonia nitrogen concentration is lower than 50mg/L in the waste water; The denitrification microbial inoculum adds in batches, preferably divides to add for 3 ~ 10 times, adds once every 1～7 day.
10. according to claim 1 or 9 described methods, it is characterized in that: the denitrification microbial inoculum adds by the volume ratio that per hour accounts for the amount of disposing of sewage 0.01% ~ 1% first, successively decrease one by one later on, each dosage successively decreases 30%～70% than the last time, when nitrate is lower than 25mg/L, stop to add.
11. method according to claim 1 is characterized in that: in the denitrification microbial inoculum, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are that the thalline volume ratio is the mixing thalline of 1:0.1 ~ 3.
12. method according to claim 1 is characterized in that: " Staphylococcus cohnis FSDN-C " is 1:0.1 ~ 10 with the ratio of " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " two bacteroids, is preferably 1:0.2 ~ 5.
13. method according to claim 1 is characterized in that: in the denitrification microbial inoculum, contain nutritive substance and preservation auxiliary agent, the volume sum of thalline accounts for 10%～60% of denitrification denitrogenation microbial inoculum cumulative volume, is preferably 20%～50%.
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| WO2021077452A1 (en) * | 2019-10-24 | 2021-04-29 | 南京大学 | Pseudomonas balearica and application thereof in treating landfill leachate membrane concentrated solution |
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| CN103374524A (en) * | 2012-04-29 | 2013-10-30 | 中国石油化工股份有限公司 | Salt-tolerant microbial agent and preparation method thereof |
| CN103374524B (en) * | 2012-04-29 | 2014-08-20 | 中国石油化工股份有限公司 | Salt-tolerant microbial agent and preparation method thereof |
| CN104560859A (en) * | 2015-01-26 | 2015-04-29 | 鹭滨环保科技（上海）有限公司 | Staphylococcus pasteuri, preparation method thereof and application in sewage treatment. |
| CN104560859B (en) * | 2015-01-26 | 2018-03-16 | 鹭滨环保科技（上海）股份有限公司 | A kind of Staphylococcus pasteuri and preparation method thereof and the application in sewage is handled |
| CN106745793A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of deep treatment method of saline sewage |
| CN106745793B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Advanced treatment method of salt-containing sewage |
| WO2021077452A1 (en) * | 2019-10-24 | 2021-04-29 | 南京大学 | Pseudomonas balearica and application thereof in treating landfill leachate membrane concentrated solution |
| CN111285473A (en) * | 2019-12-28 | 2020-06-16 | 北京翰祺环境技术有限公司 | Method for promoting quick start of denitrification and decarburization system by using high-salt-resistant strain |
| CN111285473B (en) * | 2019-12-28 | 2022-03-08 | 北京翰祺环境技术有限公司 | Method for promoting quick start of denitrification and decarburization system by using high-salt-resistant strain |
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