CN109609400B - Circulating water culture system and special microecological bactericide thereof - Google Patents
Circulating water culture system and special microecological bactericide thereof Download PDFInfo
- Publication number
- CN109609400B CN109609400B CN201811442256.2A CN201811442256A CN109609400B CN 109609400 B CN109609400 B CN 109609400B CN 201811442256 A CN201811442256 A CN 201811442256A CN 109609400 B CN109609400 B CN 109609400B
- Authority
- CN
- China
- Prior art keywords
- water
- pond
- microecological
- nitrate
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 130
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 43
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 43
- 238000009360 aquaculture Methods 0.000 claims abstract description 59
- 244000144974 aquaculture Species 0.000 claims abstract description 59
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 46
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 41
- 241000589597 Paracoccus denitrificans Species 0.000 claims abstract description 25
- 241000168225 Pseudomonas alcaligenes Species 0.000 claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 10
- 241000100611 Halomonas denitrificans Species 0.000 claims abstract description 8
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 26
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 24
- 241000206596 Halomonas Species 0.000 claims description 20
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 20
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 10
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 10
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 10
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 10
- 229920002472 Starch Polymers 0.000 claims description 10
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 10
- 239000011609 ammonium molybdate Substances 0.000 claims description 10
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 10
- 229940010552 ammonium molybdate Drugs 0.000 claims description 10
- 239000001110 calcium chloride Substances 0.000 claims description 10
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 10
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 10
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 10
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 10
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 10
- 229960001484 edetic acid Drugs 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- 239000011565 manganese chloride Substances 0.000 claims description 10
- 235000002867 manganese chloride Nutrition 0.000 claims description 10
- 229940099607 manganese chloride Drugs 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- 239000008107 starch Substances 0.000 claims description 10
- 235000019698 starch Nutrition 0.000 claims description 10
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 9
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 9
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- 229960002713 calcium chloride Drugs 0.000 claims description 7
- 229960000355 copper sulfate Drugs 0.000 claims description 7
- 239000007791 liquid phase Substances 0.000 claims description 7
- 241001057811 Paracoccus <mealybug> Species 0.000 claims description 3
- 241001459308 Pseudomonas alcaliphila Species 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 230000003134 recirculating effect Effects 0.000 abstract description 30
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 14
- 238000002474 experimental method Methods 0.000 abstract description 11
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 5
- 238000009825 accumulation Methods 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 20
- 238000012360 testing method Methods 0.000 description 18
- 230000003213 activating effect Effects 0.000 description 14
- 238000000855 fermentation Methods 0.000 description 14
- 230000004151 fermentation Effects 0.000 description 14
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 10
- -1 polypropylene Polymers 0.000 description 9
- 239000004743 Polypropylene Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 7
- 229920001155 polypropylene Polymers 0.000 description 7
- 241000252233 Cyprinus carpio Species 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 6
- 241000276707 Tilapia Species 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- 238000005273 aeration Methods 0.000 description 5
- 239000012880 LB liquid culture medium Substances 0.000 description 4
- 239000003657 drainage water Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 239000003761 preservation solution Substances 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000006004 Quartz sand Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000588986 Alcaligenes Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 206010063385 Intellectualisation Diseases 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000551 dentifrice Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a circulating water culture system and a special microecological bactericide thereof. The microecological agents include Paracoccus denitrificans, Halomonas denitrificans, Pseudomonas alcaligenes and nutrient elements for maintaining the growth of bacteria. The circulating water culture system comprises a culture pond, an anoxic pond, an aerobic pond and a filter pond, wherein the anoxic pond is positioned at the downstream of the culture pond and at the upstream of the aerobic pond. Experiments prove that the microecological bactericide prepared by the invention can be put into an anoxic pond and an aerobic pond to remove nitrate in a water body and reduce the concentration of ammonia nitrogen, nitrite and the like. Therefore, the recirculating aquaculture system and the special microecological bactericide thereof provided by the invention can not only improve the nitrate accumulation problem of a recirculating aquaculture mode, but also save water sources, reduce the aquaculture cost and are harmless to the environment. The invention has important application value.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a circulating water aquaculture system and a special microecological bactericide thereof.
Background
In the 20 th century, a recirculating aquaculture mode began to develop in parts of europe due to water shortage and increased environmental requirements. With the integration of advanced environmental engineering technology and industrial management concept, the recirculating aquaculture mode develops rapidly. In China, a recirculating aquaculture demonstration area is established in the 90 s, and with economic development and urbanization progress, a recirculating aquaculture mode is gradually accepted and supported by the nation and enterprises with the advantages of high efficiency and low pollution, and is still in a rapidly developing state at present. In addition, the demand of China on aquatic products is increasing all the time, the circulating water culture mode is regarded as an important direction of the development of modern fishery, and the characteristics of intensification, intellectualization, high density and the like of the circulating water culture mode are greatly superior to the existing extensive culture mode.
However, the water treatment method in the recirculating aquaculture mode mainly converts harmful nitrogen-containing substances in the water into nitrate with low toxicity, and because water is not changed for a long time, the nitrate in the system can be stably accumulated to quite high concentration, and after being ingested by a culture object, nitrite with high toxicity is generated under the action of microorganisms in vivo, so that the culture object generates the problems of spasm, shock, body color fading and the like. At present, the problem of nitrate accumulation is mainly solved by changing water in a recirculating aquaculture mode. In other fields, nitrate accumulation is mainly removed by ion exchange and solid-phase denitrification. However, the ion exchange method has high cost and imperfect technology, and is easily affected by chloride ions, sulfate ions and the like; the solid-phase denitrification method not only needs operators to add carbon sources at proper time, but also requires that the dissolved oxygen concentration cannot be high, so that the method has high requirements on the operation management of a culture system, and simultaneously has the problems that the nitrate removal effect of the solid-phase denitrification method is unstable, the nitrite concentration is easily induced to rise and the like. These two costly approaches are difficult to withstand in the aquaculture field.
Disclosure of Invention
The invention aims to remove nitrate in aquaculture water.
The invention firstly protects a microecological agent which can comprise paracoccus denitrificans.
The microecological agent may also include Halomonas denitrificans and Pseudomonas alcaligenes.
The microecological microbial agent can be composed of denitrifying halomonas, pseudomonas alcaligenes and paracoccus denitrificans. Among the microecological agents, denitrifying halomonas, Pseudomonas alcaligenes and Deacetobacter xylinumThe colony forming unit (cfu) number ratio of Paracoccus azonians may be (7X 10)7-7×109)cfu：(5×107-5×109)cfu：(5×107-5×109) cfu (e.g. 7X 10)8cfu：(5×107-5×109)cfu：(5×107-5×109) cfu or 7X 108cfu：5×108cfu：5×108cfu)。
Any of the aforementioned microecological agents may further comprise nutrients for maintaining the growth of bacteria (e.g., paracoccus denitrificans, halomonas denitrificans, pseudomonas alcaligenes).
The nutrient elements for maintaining bacterial growth may include starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate, and cobalt chloride.
The nutrient elements for maintaining bacterial growth may be starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate and cobalt chloride.
Any one of the microecological agents can be specifically composed of denitrifying halomonas, pseudomonas alcaligenes, paracoccus denitrificans, starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate and cobalt chloride. In the microecological bactericide, the ratio of denitrifying halomonas, Pseudomonas alcaligenes, paracoccus denitrificans, starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate and cobalt chloride can be (7 × 10)7-7×109)cfu：(5×107-5×109)cfu：(5×107-5×109) cfu: (720-880) mg: (45-55) mg: (0.009-0.011) mg: (27-33) mg: (1.8-2.2) mg: (27-33) mg: (0.09-0.11) μ g: (0.027-0.033) ng: (0.018-0.022) ng: (0.018-0.022) ng: (0.018-0.022) ng (e.g. 7X 10)8cfu：(5×107-5×109)cfu：(5×107-5×109)cfu：(720-880)mg：(45-55)mg：(0.009-0.011)mg：(27-33) mg: (1.8-2.2) mg: (27-33) mg: (0.09-0.11) μ g: (0.027-0.033) ng: (0.018-0.022) ng: (0.018-0.022) ng: (0.018-0.022) ng or 7X 108cfu：5×108cfu：5×108cfu：800mg：50mg：0.01mg：30mg：2mg：30mg：0.1μg：0.03ng：0.02ng：0.02ng：0.02ng)。
In the microecological bactericide, the active ingredients of the microecological bactericide can be viable bacteria products prepared by fermenting and culturing denitrifying halomonas, pseudomonas alcaligenes and paracoccus denitrificans, or directly using or concentrating the use or adsorbing the product by a carrier.
The content of denitrifying halomonas in the microecological bactericide can be (7 x 10)7-7×109) cfu/g (e.g., 7X 10)8cfu/g). The content of Pseudomonas alcaligenes can be (5 × 10)7-5×109) cfu/g (e.g. 5X 10)8cfu/g). The content of Paracoccus denitrificans can be (5 × 10)7-5×109) cfu/g (e.g. 5X 10)8cfu/g). The starch content may be (720-880) mg/g (e.g., 800 mg/g). The content of ethylenediaminetetraacetic acid may be (45-55) mg/g (e.g., 50 mg/g). The magnesium sulfate may be present in an amount of (0.009-0.011) mg/g (e.g., 0.01 mg/g). The disodium phosphate may be present in an amount of (27-33) mg/g (e.g., 30 mg/g). The amount of ferric sulphate may be (1.8-2.2) mg/g (e.g. 2 mg/g). The content of monopotassium phosphate can be (27-33) mg/g (e.g., 30 mg/g). The calcium chloride may be present in an amount of (0.09-0.11) μ g/g (e.g., 0.1 μ g/g). The copper sulfate content may be (0.027-0.033) ng/g (e.g. 0.03 ng/g). The content of manganese chloride can be (0.018-0.022) ng/g (such as 0.02 ng/g). The ammonium molybdate may be present in an amount of (0.018-0.022) ng/g (e.g., 0.02 ng/g). The content of cobalt chloride can be (0.018-0.022) ng/g (such as 0.02 ng/g).
Any one of the above denitrifying Halomonas can be specifically denitrifying Halomonas (Halomonas dentiricans) CGMCC 1.7484.
Any one of the above Pseudomonas alcaligenes may specifically be Pseudomonas alcaligenes (CGMCC 1.8055).
Any of the aforementioned Paracoccus denitrificans may specifically be Paracoccus denitrificans (Paracoccus Denitrificans) ATCC 13543.
Any one of the microecological bactericides can be specifically at least one of the following bactericides: 1) micro ecological bacteria agent for removing nitrate; 2) micro ecological bacteria agent for removing nitrate in water; 3) a microecological bactericide for removing nitrate in aquaculture water.
The invention also provides a method for preparing any one of the microecological bactericide, which can be used for preparing the microecological bactericide by mixing any one of the denitrifying halomonas bacterium, the alcaligenes pseudomonad, the paracoccus denitrificans, the starch, the ethylene diamine tetraacetic acid, the magnesium sulfate, the disodium hydrogen phosphate, the ferric sulfate, the potassium dihydrogen phosphate, the calcium chloride, the copper sulfate, the manganese chloride, the ammonium molybdate and the cobalt chloride according to the proportion.
In the method for preparing the microecological bactericide, the denitrifying halomonas, the pseudomonas alcaligenes and the paracoccus denitrificans can be fermented and cultured respectively to prepare fermentation liquor, and then the fermentation liquor is mixed with other components according to the proportion. The culture medium for fermentation culture can be LB liquid culture medium. The temperature of the fermentation culture can be 35-39 deg.C (such as 35-37 deg.C, 37-39 deg.C, 35 deg.C, 37 deg.C or 39 deg.C). The fermentation culture time can be 36-60h (such as 36-48h, 48-60h, 36h, 48h or 60 h).
The invention also protects the application of any one of the microecological agents, which can be at least one of a1) -a 4): a1) removing nitrate; a2) removing nitrate in water; a3) preparing a product from which nitrate is removed; a4) preparing a product for removing nitrate in water.
In the above application, the nitrate is removed or the nitrate in the water is removed by at least one of anaerobic denitrification, aerobic denitrification and assimilation.
In the application, the nitrate in the water can be removed specifically by removing nitrate in the water body of aquaculture.
The invention also provides a method for removing nitrate, which comprises the following steps: adding any one of the microecological agents into a liquid phase system containing nitrate nitrogen, thereby removing nitrate in the liquid phase system.
In the above method, the liquid phase system containing nitrate nitrogen may specifically be a liquid phase system containing 30mg/L or more of nitrate nitrogen.
In the method, the liquid phase system can be a water body for aquaculture.
The invention also discloses a circulating water culture system, which comprises a culture pond, an aerobic pond and a filter pond; the recirculating aquaculture system may further comprise an anoxic tank; the anoxic pond is positioned at the downstream of the culture pond and at the upstream of the aerobic pond.
In the above recirculating aquaculture system, the volume of the anoxic pond may be 1/(3-5) (e.g. 1/3, 1/4 or 1/5) of the volume of the aquaculture pond.
In the recirculating aquaculture system, the anoxic pond can consist of at least one treatment pond; each treatment tank can comprise a tank body, a water inlet and a water outlet; one of the water inlet and the water outlet is positioned at the upper part of the side wall of the tank body, and the other is positioned at the bottom of the side wall of the tank body.
In the above recirculating aquaculture system, the treatment tank may further be provided with a water treatment device for reducing hydraulic retention time.
The water treatment device for reducing the hydraulic retention time can be a polypropylene honeycomb inclined plate or a polypropylene honeycomb inclined pipe.
In the above-mentioned recirculating aquaculture system, the described aerobic pool can be equipped with aeration system or spherical polyethylene filler (for the purpose of keeping aeration).
In the above recirculating aquaculture system, the filter tank is filled with filter material (such as cobblestone, gravel, and quartz sand).
In one embodiment of the invention, the anoxic tank is composed of 3 treatment tanks. The 3 rd treatment pool is provided with a polypropylene honeycomb inclined plate.
In another embodiment of the invention, the anoxic tank consists of 1 treatment tank, and a polypropylene honeycomb inclined plate is arranged in the treatment tank.
The invention also discloses a method for removing nitrate in aquaculture water, which can be S1) or S2) or S3).
S1) adopting any one of the circulating water culture systems to carry out aquaculture, adding any one of the microecologics into the water body, and removing nitrate in the water body.
S2) adopting any one of the circulating water culture systems to carry out aquaculture, adding any one of the microecologics into the anoxic pond and/or the aerobic pond, and removing nitrate in the water body.
S3) adding any microecological bactericide into the aquaculture water body to remove nitrate in the water body.
In the above S1), the "adding any one of the microecological agents to the water body" may specifically be adding any one of the microecological agents when the nitrate nitrogen concentration in the water body reaches more than 30 mg/L.
In the above S2), the step of "adding any one of the microecologics into the anoxic tank and/or the aerobic tank" may specifically be that when the nitrate nitrogen concentration in the water body reaches to 30mg/L or more, any one of the microecologics is added into the anoxic tank and/or the aerobic tank (any one of the microecologics may be activated first). The activation mode is as follows: mixing 1 part by mass of any one of the microecological agents with 15 parts by mass of water (such as tap water or river water), and standing for 2 hours or more (such as 3 hours). The microecological bactericide can be put into half of the anoxic pond and half of the aerobic pond, or only the anoxic pond or only the aerobic pond, or the proportion of the microecological bactericide to be put into the anoxic pond and the aerobic pond can be determined according to the volumes of the anoxic pond and the aerobic pond.
In the above S3), the step of "adding any one of the microecological agents to the aquaculture water" may be specifically adding any one of the microecological agents when the nitrate nitrogen concentration in the aquaculture water reaches more than 30 mg/L.
The dosage of any one of the microecological agents can be specifically 200-3Water body (such as 200-3Water body, 400-3Water body, 500-3Water body, 200g/m3Water body, 250g/m3Water body, 400g/m3Water body, 500g/m3Water body or 600g/m3A body of water).
In order to make the nitrate removal effect more durable, any of the microecological agents can be added at regular intervals (e.g., 30 days). In order to increase the dominance of the strains in the microbial community in the microecological agent, the administration of any of the microecological agents can be carried out 1 or more times (e.g., 2 times). In bookIn one embodiment of the invention, the following operations are performed every 30 days: firstly, any one of the microecological microbial agents is added (the adding amount is 500 g/m)3Water body), 3 days later, adding any one of the microecological agents (the adding amount is 250 g/m)3A body of water).
Experiments prove that the micro-ecological bacteria agent prepared by the invention can play a role in denitrification in aerobic and anaerobic states, and can remove nitrate in water body by three modes of anaerobic denitrification, aerobic denitrification and assimilation, so that the micro-ecological bacteria agent can be put into an anoxic tank and an aerobic tank at the same time. Meanwhile, the method is simple to operate and low in cost, and can reduce the concentration of ammonia nitrogen, nitrite and the like while removing nitrate. Therefore, the recirculating aquaculture system and the special microecological bactericide thereof can improve the nitrate accumulation problem of a recirculating aquaculture mode, save water sources, reduce the aquaculture cost and are harmless to the environment. The invention optimizes the circulating water culture mode and has important application value.
Drawings
FIG. 1 shows a water circulation process of a recirculating aquaculture system.
FIG. 2 shows the water quality change of the recirculating aquaculture system of example 3.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention.
The experimental procedures in the following examples are conventional unless otherwise specified.
The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.
The denitrifying Halomonas (Halomonas dentifrices) CGMCC 1.7484 and Pseudomonas alcaligenes (Pseudomonas alcalophila) CGMCC 1.8055 in the following examples are both stored in China general microbiological culture Collection center (CGMCC for short, address: Beijing area sunward area, North Cheng Xilu No. 1, Japan, Zip code 100101), and are publicly available from the China general microbiological culture Collection center. Hereinafter, Halomonas denitrificans (Halomonas dentiricans) CGMCC 1.7484 is abbreviated as Halomonas denitrificans, and Pseudomonas alcaligenes (Pseudomonas alcalophila) CGMCC 1.8055 is abbreviated as Pseudomonas alcaligenes.
Paracoccus denitrificans (Paracoccus denitificas) ATCC13543 in the examples described below was deposited in American Type Culture Collection (ATCC for short, address: American Type Culture Collection (ATCC)10801University Boulevard Manassas, VA 20110USA), and was publicly available from the American Type Culture Collection. Hereinafter, Paracoccus denitrificans (Paracoccus Denitrificans) ATCC13543 is simply referred to as Paracoccus denitrificans.
Example 1 preparation of microecological Agents
First, obtaining fermentation liquor
1. Obtaining of fermentation liquor of denitrifying halomonas
(1) 0.1mL of the Halomonas denitrificans preservation solution is inoculated in a 200mLLB liquid culture medium and subjected to shaking culture at 37 ℃ and 200rpm for 48 hours to obtain a seed solution.
(2) And (3) after the step (1) is finished, completely inoculating the seed liquid into a 40L LB liquid culture medium, and performing shaking culture at 37 ℃ and 200rpm for 48 hours to obtain the zymotic fluid of the denitrifying halomonas.
2. Obtaining fermentation liquor of pseudomonas alcaligenes
(1) Inoculating 0.1mL of Pseudomonas alcaligenes preservation solution into 200mLLB liquid culture medium, and performing shake culture at 37 deg.C and 200rpm for 48h to obtain seed solution.
(2) And (2) after the step (1) is finished, completely inoculating the seed liquid into a 40L LB liquid culture medium, and carrying out shaking culture at 37 ℃ and 200rpm for 48h to obtain the fermentation liquid of the pseudomonas alcaligenes.
3. Obtaining fermentation liquor of paracoccus denitrificans
(1) 0.1mL of paracoccus denitrificans preservation solution is inoculated in 200mLLB liquid culture medium and is subjected to shaking culture at 37 ℃ and 200rpm for 48 hours to obtain seed solution.
(2) And (3) after the step (1) is finished, completely inoculating the seed liquid into a 40L LB liquid culture medium, and carrying out shaking culture at 37 ℃ and 200rpm for 48h to obtain paracoccus denitrificans fermentation liquid.
Preparation of microbial ecological agent
The fermentation liquor of the denitrifying halomonas obtained in the step one and the pseudomonas alcaligenes are fermentedMixing the fermentation solution, the paracoccus denitrificans fermentation solution and nutrient element mixed powder (consisting of starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate and cobalt chloride) to obtain the microecological bactericide. The content of denitrifying halomonas in the microecological agent is 7 × 108cfu/g, content of Pseudomonas alcaligenes of 5X 108cfu/g, content of paracoccus denitrificans 5 × 108cfu/g, the content of starch is 800mg/g, the content of ethylene diamine tetraacetic acid is 50mg/g, the content of magnesium sulfate is 0.01mg/g, the content of disodium hydrogen phosphate is 30mg/g, the content of ferric sulfate is 2mg/g, the content of monopotassium phosphate is 30mg/g, the content of calcium chloride is 0.1 mu g/g, the content of copper sulfate is 0.03ng/g, the content of manganese chloride is 0.02ng/g, the content of ammonium molybdate is 0.02ng/g, and the content of cobalt chloride is 0.02 ng/g.
The heavy metal content in the microecological bactericide is low, and the microecological bactericide is mainly absorbed by microorganisms, so that the heavy metal content of a cultured object cannot exceed the standard.
Example 2 establishment of a recirculating aquaculture System and use of Microecological Agents in the recirculating aquaculture System
First, establishment of recirculating aquaculture system
In the traditional recirculating aquaculture system, water in a culture pond enters a solid-liquid separation system and then enters an aeration biological filter, and an independent and definite anoxic zone does not exist. Through a large number of experiments, the inventor of the invention establishes a circulating water aquaculture system as shown in figure 1 (the water circulation process is that the water discharged from an aquaculture pond enters an anoxic pond, the water discharged from the anoxic pond enters an aerobic pond, the water discharged from the aerobic pond enters a filter pond, and the water discharged from the filter pond enters the aquaculture pond). Compared with the traditional circulating water culture system, the circulating water culture system established by the invention is mainly characterized in that an anoxic pond is arranged at the downstream of the culture pond and at the upstream of the aerobic pond; the microecological bactericide prepared in the embodiment 1 is added into the anoxic pond regularly or irregularly by detecting the quality of the water body. The anoxic pond can achieve two purposes, namely facilitating the precipitation of solid pollutants in the water body and ammoniating nitrogen-containing organic matters in the water body.
The volume of the anoxic pond is 1/4 of the volume of the culture pond, and the anoxic pond is positioned at the downstream of the culture pond and at the upstream of the aerobic pond. The concrete structure is as follows: the anoxic tank is respectively a treatment tank 1, a treatment tank 2 and a treatment tank 3 from upstream to downstream; the volume of the treatment tank 1 is 1/10 of the volume of the anoxic tank, the upper part of the side wall of the treatment tank 1 adjacent to the culture tank is provided with a water inlet, and the bottom of the other side wall of the treatment tank is provided with a water outlet (the water outlet is also the water inlet of the treatment tank 2); the volume of the treatment tank 2 is 4/10 of the volume of the anoxic tank, and the upper part of the side wall of the treatment tank 2 adjacent to the treatment tank 3 is provided with a water outlet (the water outlet is also the water inlet of the treatment tank 3); the volume of the treatment tank 3 is 5/10 of the volume of the anoxic tank, and the bottom of the side wall of the treatment tank 3 adjacent to the aerobic tank is provided with a water outlet. The treatment tank 3 is also provided with a polypropylene honeycomb inclined plate for shortening the settling time and reducing the hydraulic retention time required by the treatment tank.
During operation, the drainage of the culture pond flows into the treatment pond 1 through the water inlet, flows into the treatment pond 2 through the water outlet of the treatment pond 1, flows into the treatment pond 3 through the water outlet of the treatment pond 2, and flows into the aerobic pond through the water outlet of the treatment pond 3.
The arrangement of a plurality of treatment ponds of the anoxic pond is beneficial to the precipitation of pollutants such as fish manure, residual feed and the like, and provides various environments for microorganisms. The first treatment tank mainly plays a role in buffering and transition, the subsequent treatment tanks form a facultative-anoxic microenvironment, and pollutants are decomposed under the action of microecological bactericides to form substances with smaller molecular weight, such as ammonia nitrogen, short-chain organic acid and the like.
Use in circulating water aquaculture system established in step one by using microecologics
Taking 1 part by mass of microecological bactericide, adding 15 parts by mass of tap water or river water, and standing for 3h (for activation) to obtain an activation solution.
The use method of the circulating water culture system established in the step one of the microecological bactericide comprises the following steps:
1. when the ammonia nitrogen concentration of the recirculating aquaculture system established in the step one is below 2mg/L, the nitrite nitrogen concentration is below 1mg/L and the nitrate nitrogen concentration is above 30mg/L, respectively adding the activating solution into the anoxic pond and the aerobic pond, wherein the adding amount is 500g of microecological bactericide/m3Culture waterAnd (3) a body.
2. On the 3 rd day after the step 1 is finished, respectively adding the activating liquid into the anoxic tank and the aerobic tank, wherein the adding amount is 250g of microecological bactericide/m3And (5) cultivating a water body.
The purpose of supplementing the microecological bactericide is to increase the advantages of strains in the microecological bactericide in microbial communities, so that the nitrate removal effect is more obvious and more durable.
3. And (5) on the 5 th day after the step (1) is finished, sludge in the anoxic pond and the aerobic pond can be properly removed, and the improvement of the water quality of the recirculating aquaculture system is facilitated.
Example 3, microecological agent prepared in example 1 and application of recirculating aquaculture system established in example 2
Taking 1 part by mass of microecological bactericide, adding 15 parts by mass of tap water or river water, and standing for 3h (for activation) to obtain an activation solution.
One, application one
The inventor of the invention establishes the recirculating aquaculture system in the Shenzhen Longsentry region Huada agricultural laboratory in the embodiment 2. The recirculating aquaculture system consists of a culture area and a water treatment area. The culture area consists of two cylindrical culture ponds, and the volume of each culture pond is 1m3. The water treatment area is 0.5m3Anoxic pond, 1m3And the aerobic pool is 0.5m3The filter tank. The anoxic tank is divided into three grids which correspond to the treatment tank 1, the treatment tank 2 and the treatment tank 3 in sequence, and the treatment tank 3 is provided with a polypropylene honeycomb inclined plate. The aerobic tank is filled with spherical polyethylene filler to keep aeration. The filtering material such as cobblestones, broken stones and the like is placed in the filtering tank.
1. On the 1 st day of the experiment, tilapia with the weight of about 30 g/tail is thrown for culture. The culture density is 10kg/m3. The feed amount put in each culture pond is 300 g/day. The dissolved oxygen in the culture pond is 2-2.5 mg/L.
2. And (5) observing the water body condition on the 40 th day of the test. The result shows that a large amount of solid wastes such as fish manure, residual feed and the like in the culture area are accumulated in the anoxic pond in the form of precipitated sludge and floating sludge; the water bodies of the aerobic tank and the filter tank are clear.
3. During the test, the ammonia nitrogen concentration, the nitrite nitrogen concentration and the nitrate nitrogen concentration of the effluent of the culture pond are respectively detected (the detection methods of the ammonia nitrogen, the nitrite nitrogen and the nitrate nitrogen are respectively according to HJ535-2009, GB7493-87 and GB7480-87 standards issued by the ministry of environmental protection of China), and the treatment is carried out according to the nitrate nitrogen concentration.
Test to 49 days: the ammonia nitrogen concentration and the nitrite nitrogen concentration of the drainage water of the culture pond have high peaks in sequence.
Test day 50-70: the quality of the discharged water of the culture pond is stable, the concentration of ammonia nitrogen is between 0 and 3mg/L, the concentration of nitrite nitrogen is basically zero, and the concentration of nitrate nitrogen is continuously accumulated.
Day 71 of the experiment: the ammonia nitrogen concentration of the drainage water of the culture pond is 1.2mg/L, the nitrite nitrogen concentration is 0.2mg/L, and the nitrate nitrogen concentration is 49.2 mg/L. The microecological agent prepared in example 1 was added for treatment since the nitrate nitrogen concentration was high. The specific treatment method comprises the following steps: 8kg of activating solution is added into an anoxic tank, and 8kg of activating solution is added into an aerobic tank.
Day 72 of the experiment: the concentration of nitrate nitrogen in the effluent of the culture pond is 31.8mg/L (17.4 mg/L lower than that of the 71 th day of the test), the concentration of ammonia nitrogen is 2.0mg/L (slightly higher than that of the 71 th day of the test), and the concentration of nitrite nitrogen is 1.2mg/L (slightly higher than that of the 71 th day of the test).
Test day 73 and 74: the nitrate nitrogen concentration of the effluent of the culture pond is 33.5mg/L and 36.3mg/L respectively (the nitrate nitrogen accumulation speed is basically consistent with that before the microecological bactericide is added), the ammonia nitrogen concentration is 0.6mg/L and 1.9mg/L respectively, and the nitrite nitrogen concentration is 0.2 mg/L. Therefore, after the microecological bactericide is added, the obvious deterioration of water quality and the quick rebound of nitrate are not caused.
Day 74 of the experiment: adding a microecological microbial agent for treatment. The specific treatment method comprises the following steps: 4kg of activating solution is added into an anoxic tank, and 4kg of activating solution is added into an aerobic tank.
Test day 75: the concentration of nitrate nitrogen in the drainage water of the culture pond is 28.3mg/L, the concentration of ammonia nitrogen is 0.8mg/L, and the concentration of nitrite nitrogen is 0.9 mg/L.
Then, adding the microecological bactericide according to the method every 30 days (such as the test day 101, the test day 131 and the test day 161, adding 8kg of the activating solution into the anoxic tank and 8kg of the activating solution into the aerobic tank, and the test day 104, the test day 134 and the test day 164, adding 4kg of the activating solution into the anoxic tank and 4kg of the activating solution into the aerobic tank), and detecting the ammonia nitrogen concentration, the nitrite nitrogen concentration and the nitrate nitrogen concentration of the drainage water of the culture tank.
The water quality change of the culture pond drainage is shown in figure 2: the nitrate concentration is reduced after the micro-ecological bacteria agent is added. Nitrate without adding microecological bactericide is continuously accumulated. The result shows that the nitrate concentration is controlled to a certain degree after the microecological bactericide is added.
And detecting the heavy metal content, growth, reproduction, metabolism and other indexes of the tilapia during the whole process. The result shows that the heavy metal content of the tilapia does not exceed the standard, the indexes of growth, reproduction, metabolism and the like are completely normal, and the tilapia has no obvious difference compared with the tilapia cultured by a recirculating aquaculture system without adding the microecological bactericide.
Second, apply second
The inventor of the invention establishes the recirculating aquaculture system in the Shenzhen Dapeng new region in the embodiment 2. The recirculating aquaculture system is a rectangular cement pond with the length of 3m multiplied by 5m multiplied by 0.6m (respectively corresponding to the length, the width and the height), and one half of the rectangular cement pond is used as an aquaculture area (4.5 m)3) Half as water treatment area (4.5 m)3)。
The water treatment area is divided into anoxic areas (1.5 m) by partition plates3) Aerobic zone (1.5 m)3) A filtering area (1.5 m)3). The anoxic zone is provided with a polypropylene honeycomb sloping plate. The aerobic zone is provided with an aeration system, and the dissolved oxygen is between 2 and 3 mg/L. And quartz sand is put in the filtering area. The partition board has holes, the water in the culture area flows through the anoxic area, the aerobic area and the filtering area in sequence, and the water in the filtering area is pumped by a water pump to be supplemented to the culture area.
1. On the 1 st day of the experiment, koi with the weight of about 96 g/tail is added for cultivation. The culture density is 5kg/4.5m3(namely 5kg of koi is put into the cultivation area). The feed amount put in the breeding area is 150 g/day.
2. Day 80 of the experiment: the nitrate nitrogen concentration of the water body is 75.4 mg/L. The microecological agent prepared in example 1 was added for treatment since the nitrate nitrogen concentration was high. The specific treatment method comprises the following steps: and adding 24kg of activating solution into the anoxic tank, and adding 24kg of activating solution into the aerobic tank.
3. Test day 81, 82 and 83: the nitrate nitrogen concentration of the water body is respectively 50.3mg/L, 52.7mg/L and 53.9 mg/L.
4. Day 83 of the experiment: adding a microecological microbial agent for treatment. The specific treatment method comprises the following steps: 12kg of activating solution is added into an anoxic tank, and 12kg of activating solution is added into an aerobic tank.
5. Test day 84, 85 and 86: the nitrate nitrogen concentration of the water body is respectively 38.4mg/L, 40.5mg/L and 41.7mg/L, and the ammonia nitrogen concentration and the nitrite concentration are both between 0 and 2 mg/L.
The result shows that the survival rate of the koi reaches 96 percent, and the nitrate nitrogen concentration of the water body is obviously reduced.
And detecting the heavy metal content, growth, reproduction, metabolism and other indexes of the fancy carp in the whole process. The result shows that the content of heavy metal of the fancy carp does not exceed the standard, the indexes of growth, reproduction, metabolism and the like are completely normal, and no obvious difference exists in comparison with the fancy carp cultured by a circulating water culture system without adding the micro-ecological bacteria agent.
Claims (9)
1. A microecological bactericide comprises Paracoccus denitrificans (Paracoccus Denitrificans) ATCC13543, Halomonas denitrificans (Halomonas Denitrificans) CGMCC 1.7484 and Pseudomonas alcaligenes (Pseudomonas alcalophila) CGMCC 1.8055.
2. The microecological agent according to claim 1, wherein: the microecological agent also comprises nutrient elements for maintaining the growth of bacteria.
3. The microecological agent according to claim 2, wherein: the nutrient elements for maintaining bacterial growth include starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate and cobalt chloride.
4. Such asThe microecological agent according to claim 3, wherein: in the microecological bactericide, the ratio of denitrifying Halomonas (Halomonas densitificans) CGMCC 1.7484, Pseudomonas alcaligenes (CGMCC 1.8055), Paracoccus denitrificans (Paracoccus densitificans) ATCC13543, starch, ethylene diamine tetraacetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulfate, manganese chloride, ammonium molybdate and cobalt chloride is (7 x 10)7-7×109）cfu：（5×107-5×109）cfu：（5×107-5×109）cfu：（720-880）mg：（45-55）mg：（0.009-0.011）mg：（27-33）mg：（1.8-2.2）mg：（27-33）mg：（0.09-0.11）μg：（0.027-0.033）ng：（0.018-0.022）ng：（0.018-0.022）ng：（0.018-0.022）ng。
5. Use of the microecological agent according to any one of claims 1 to 4, in at least one of a1) -a 4): a1) removing nitrate; a2) removing nitrate in water; a3) preparing a product from which nitrate is removed; a4) preparing a product for removing nitrate in water.
6. A method for removing nitrate comprising the steps of: adding the microecological agent as claimed in any one of claims 1 to 4 into a liquid phase system containing nitrate nitrogen, and removing nitrate in the liquid phase system.
7. A method for removing nitrate in aquaculture water, which is to adopt a circulating water aquaculture system to carry out aquaculture, and the microecological bactericide of any one of claims 1 to 4 is added into the water to remove the nitrate in the water;
the circulating water culture system comprises a culture pond, an aerobic pond, a filter pond and an anoxic pond; the anoxic pond is positioned at the downstream of the culture pond and at the upstream of the aerobic pond; the anoxic tank consists of at least one treatment tank; each treatment tank comprises a tank body, a water inlet and a water outlet; one of the water inlet and the water outlet is positioned at the upper part of the side wall of the tank body, and the other is positioned at the bottom of the side wall of the tank body.
8. A method for removing nitrate in aquaculture water, which is to adopt a circulating water aquaculture system to carry out aquaculture, and the microecological bactericide of any one of claims 1 to 4 is added into an anoxic pond and/or an aerobic pond to remove nitrate in the water;
the circulating water culture system comprises a culture pond, an aerobic pond, a filter pond and an anoxic pond; the anoxic pond is positioned at the downstream of the culture pond and at the upstream of the aerobic pond; the anoxic tank consists of at least one treatment tank; each treatment tank comprises a tank body, a water inlet and a water outlet; one of the water inlet and the water outlet is positioned at the upper part of the side wall of the tank body, and the other is positioned at the bottom of the side wall of the tank body.
9. A method for removing nitrate in aquaculture water, which is to add the microecological bactericide of any one of claims 1 to 4 into the aquaculture water to remove the nitrate in the water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811442256.2A CN109609400B (en) | 2018-11-29 | 2018-11-29 | Circulating water culture system and special microecological bactericide thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811442256.2A CN109609400B (en) | 2018-11-29 | 2018-11-29 | Circulating water culture system and special microecological bactericide thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109609400A CN109609400A (en) | 2019-04-12 |
| CN109609400B true CN109609400B (en) | 2021-10-15 |
Family
ID=66005755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811442256.2A Active CN109609400B (en) | 2018-11-29 | 2018-11-29 | Circulating water culture system and special microecological bactericide thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109609400B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115093021B (en) * | 2022-07-28 | 2022-12-30 | 宁波水思清环境科技有限公司 | Sewage treatment agent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071141A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Denitrified microbial agent as well as preparation method and application thereof |
| CN102911897A (en) * | 2012-10-11 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Cultural method for paracoccus denitrificans and application of same to purifying aquaculture water |
| CN203194328U (en) * | 2013-03-13 | 2013-09-18 | 福州开发区三水环保科技有限公司 | Integrated circulating water aquaculture purifier |
-
2018
- 2018-11-29 CN CN201811442256.2A patent/CN109609400B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071141A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Denitrified microbial agent as well as preparation method and application thereof |
| CN102911897A (en) * | 2012-10-11 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Cultural method for paracoccus denitrificans and application of same to purifying aquaculture water |
| CN203194328U (en) * | 2013-03-13 | 2013-09-18 | 福州开发区三水环保科技有限公司 | Integrated circulating water aquaculture purifier |
Non-Patent Citations (2)
| Title |
|---|
| Closed water recirculating system for fish rearing equipped with bioreactor capable of simultaneous nitrification and denitrification;Hiroaki Uemoto et al.;《Biological Sciences in Space》;19991231;第13卷(第4期);第341-347页 * |
| 好氧反硝化复合菌剂的研制及其在海水养殖废水处理中的应用;陈进斌 等;《湖北农业科学》;20170630;第56卷(第11期);第2049-2051页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109609400A (en) | 2019-04-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103896407B (en) | A kind of quick startup, biofilm carbon antimicrobial composition process for purifying water | |
| CN103373759B (en) | Method for denitrifying wastewater generated in process of producing FCC (fluid catalytic cracking) catalyst | |
| CN104030441B (en) | Three-phase biologic fluidized-bed and the sewage water treatment method of a kind of horizontal | |
| CN103373762A (en) | Biological denitrification method for salt-containing sewage | |
| CN101343119B (en) | Zeolite-acorus calamus artificial wet land sewage treatment system and treatment method | |
| CN110902948B (en) | Rural distributed domestic sewage treatment process and system for strengthening phosphorus removal and recovery | |
| AU2018263286B2 (en) | Water treatment method | |
| CN103373769B (en) | Method for biologically purifying industrial circulating water | |
| CN103373767B (en) | Method for biologically denitrifying high-salinity sewage generated in production process of catalysts | |
| CN209872514U (en) | Composite ecological reactor based on A2O | |
| CN105585133A (en) | Bio-denitrification method for high-salt-content wastewater discharged from catalyst production process | |
| CN109609400B (en) | Circulating water culture system and special microecological bactericide thereof | |
| CN208234621U (en) | The multistage composite biofilm reactor in situ for administering black and odorous water | |
| CN203938524U (en) | A kind of horizontal is three-phase biologic fluidized-bed | |
| CN105600945A (en) | Method for treating sewage by utilizing compound bacteria | |
| CN109055252A (en) | A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations and preparation method thereof | |
| CN107487938A (en) | A kind of rural sewage treatment process | |
| CN109161513B (en) | Sphingobacterium and application thereof | |
| CN116924605A (en) | Mine acid wastewater ecological treatment system and treatment method thereof | |
| CN103373761B (en) | Method for treating industrial circulating water | |
| CN116395861A (en) | Active self-sustaining liquid denitrification bacteria-containing material and preparation method and application thereof | |
| CN109678302A (en) | A kind of dystopy governing system of eutrophic lake | |
| CN109942154A (en) | The method and system of the simultaneous aerobic FBC technical treatment sewage that is coupled of biology | |
| CN103373768B (en) | Method for reducing biological slime in circulating water system | |
| CN106754453B (en) | Enrichment culture method of function-enhanced denitrification microorganisms |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240401 Address after: 518000, 5th floor, Building 11, Beishan Industrial Zone, No. 146 Beishan Road, Yangang Community, Yantian Street, Yantian District, Shenzhen City, Guangdong Province Patentee after: Shenzhen Huayouyi Biotechnology Co.,Ltd. Country or region after: China Address before: 518116 No. 7, Pengfei Road, Pengcheng community, Dapeng office, Dapeng new area, Shenzhen, Guangdong Patentee before: SHENZHEN HUADA AGRICULTURAL APPLICATION Research Institute Country or region before: China |