CN109609400A - A kind of circulating water culture system and its dedicated microecological microbial agent - Google Patents
A kind of circulating water culture system and its dedicated microecological microbial agent Download PDFInfo
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- CN109609400A CN109609400A CN201811442256.2A CN201811442256A CN109609400A CN 109609400 A CN109609400 A CN 109609400A CN 201811442256 A CN201811442256 A CN 201811442256A CN 109609400 A CN109609400 A CN 109609400A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 140
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 81
- 230000000813 microbial effect Effects 0.000 title claims abstract description 80
- 238000009360 aquaculture Methods 0.000 claims abstract description 47
- 244000144974 aquaculture Species 0.000 claims abstract description 47
- 229910002651 NO3 Inorganic materials 0.000 claims abstract description 43
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 43
- 241000589597 Paracoccus denitrificans Species 0.000 claims abstract description 25
- 241000168225 Pseudomonas alcaligenes Species 0.000 claims abstract description 22
- 241000206596 Halomonas Species 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 230000012010 growth Effects 0.000 claims abstract description 10
- 235000015097 nutrients Nutrition 0.000 claims abstract description 10
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 24
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 23
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 20
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 10
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 10
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 10
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 10
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 10
- 229960001484 edetic acid Drugs 0.000 claims description 10
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 10
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- 239000011565 manganese chloride Substances 0.000 claims description 10
- 235000002867 manganese chloride Nutrition 0.000 claims description 10
- 229940099607 manganese chloride Drugs 0.000 claims description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 9
- 229920002472 Starch Polymers 0.000 claims description 9
- 239000001110 calcium chloride Substances 0.000 claims description 9
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 9
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 9
- 239000008107 starch Substances 0.000 claims description 9
- 235000019698 starch Nutrition 0.000 claims description 9
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 8
- 239000011609 ammonium molybdate Substances 0.000 claims description 8
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 8
- 229940010552 ammonium molybdate Drugs 0.000 claims description 8
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000007791 liquid phase Substances 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 5
- 150000003839 salts Chemical group 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 26
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 14
- 238000009825 accumulation Methods 0.000 abstract description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 19
- 230000003213 activating effect Effects 0.000 description 16
- 238000000855 fermentation Methods 0.000 description 11
- 230000004151 fermentation Effects 0.000 description 11
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 9
- 239000002068 microbial inoculum Substances 0.000 description 9
- -1 phosphoric acid hydrogen Chemical class 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000252233 Cyprinus carpio Species 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 229920001155 polypropylene Polymers 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000100611 Halomonas denitrificans Species 0.000 description 3
- 241000276701 Oreochromis mossambicus Species 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 3
- 241001459308 Pseudomonas alcaliphila Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000006004 Quartz sand Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000035611 feeding Effects 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 238000004176 ammonification Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a kind of circulating water culture system and its dedicated microecological microbial agents.Microecological microbial agent includes Paracoccus denitrificans, denitrification Halomonas, Pseudomonas alcaligenes and the nutrient for maintaining bacterial growth.Circulating water culture system includes aquaculture pond, anoxic pond, aerobic tank and filtering ponds, and anoxic pond is located at the upstream in the downstream of aquaculture pond, aerobic tank.Test proves, microecological microbial agent prepared by the present invention is launched to the concentration that can be removed water body nitrate in anoxic pond and aerobic tank, while can reduce ammonia nitrogen, nitrite etc..It can be seen that circulating water culture system provided by the invention and its dedicated microecological microbial agent can not only improve the Nitrate Accumulation problem of circulating water cultivation mode, but also saves water source, reduces aquaculture cost, environmental sound.The present invention has important application value.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of circulating water culture system and its dedicated micro-ecological bacterial
Agent.
Background technique
20th century, Some European area start to develop circulating water cultivation mode due to water shortage and the raising of environmental requirement.
With the involvement of advanced environment project and factory management idea, circulating water cultivation mode is rapidly developed.China was in 90 years
In generation, begins setting up circulating water cultivation demonstration area, with economic development and urbanization process, circulating water cultivation mode with its high efficiency,
The advantages of low pollution, obtains the approval and support of country and enterprise gradually, is also in rapid development state now.In addition, China is right
The demand of aquatic products is incremented by always, and circulating water cultivation mode is considered as the important directions of Modern Fishery development, intensive, intelligence
The features such as change, high density, is better than existing extensive aquaculture model significantly.
However, that the harmful nitrogen substance of water body is mainly converted into toxicity is lower for circulating water cultivation mode method for treating water
Nitrate, due to not changing water for a long time, nitrate can run up to quite high concentration steadily in system, after cultivation object intake,
In vivo under microbial action, the nitrite being more toxic is generated, causes cultivation object to generate spasm, shock, body colour thin out
The problems such as.Circulating water cultivation mode mainly solves the problems, such as Nitrate Accumulation by changing water at present.In other fields, nitrate
Accumulation is mainly removed using ion-exchange and solid phase denitrification method.But ion-exchange higher cost, technology is not perfect,
And it is easy to be influenced by chloride ion, sulfate ion etc.；Solid phase denitrification method not only needs operator to add carbon source in due course, and
And requiring oxyty high, this has higher requirements to cultivating system operational management, while the nitrate of solid phase denitrification method
Removal effect is unstable, is easy to induce the problems such as nitrite concentration increases.Aquaculture field is difficult to bear both costs
High method.
Summary of the invention
The purpose of the present invention is the nitrate in removal aquaculture system.
The present invention protects a kind of microecological microbial agent first, may include Paracoccus denitrificans.
The microecological microbial agent may also include denitrification Halomonas and Pseudomonas alcaligenes.
The microecological microbial agent can be specifically made of denitrification Halomonas, Pseudomonas alcaligenes and Paracoccus denitrificans.Institute
It states in microecological microbial agent, denitrification Halomonas, Pseudomonas alcaligenes and the Colony Forming Unit of Paracoccus denitrificans (cfu) number
Than that can be (7 × 107-7×109) cfu:(5 × 107-5×109) cfu:(5 × 107-5×109) cfu (such as 7 × 108Cfu:(5 ×
107-5×109) cfu:(5 × 107-5×109) cfu or 7 × 108Cfu:5 × 108Cfu:5 × 108cfu)。
Any of the above-described microecological microbial agent may also include for maintaining bacterium (such as Paracoccus denitrificans, denitrification salt unit cell
Bacterium, Pseudomonas alcaligenes) growth nutrient.
It is described to be used to maintain the nutrient of bacterial growth to may include starch, ethylenediamine tetra-acetic acid, magnesium sulfate, phosphoric acid hydrogen two
Sodium, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulphate, manganese chloride, ammonium molybdate and cobalt chloride.
It is described to be used to maintain the nutrient of bacterial growth specifically be by starch, ethylenediamine tetra-acetic acid, magnesium sulfate, phosphoric acid hydrogen
Disodium, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulphate, manganese chloride, ammonium molybdate and cobalt chloride composition.
Any of the above-described microecological microbial agent specifically can by denitrification Halomonas, Pseudomonas alcaligenes, Paracoccus denitrificans,
Starch, ethylenediamine tetra-acetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulphate, manganese chloride, molybdenum
Sour ammonium and cobalt chloride composition.In the microecological microbial agent, denitrification Halomonas, Pseudomonas alcaligenes, Paracoccus denitrificans, shallow lake
Powder, ethylenediamine tetra-acetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulphate, manganese chloride, molybdic acid
The ratio of ammonium and cobalt chloride can be (7 × 107-7×109) cfu:(5 × 107-5×109) cfu:(5 × 107-5×109) cfu:
(720-880) mg:(45-55) mg:(0.009-0.011) mg:(27-33) mg:(1.8-2.2) mg:(27-33) mg:(0.09-
0.11) μ g:(0.027-0.033) ng:(0.018-0.022) ng:(0.018-0.022) ng:(0.018-0.022) ng (such as 7 ×
108Cfu:(5 × 107-5×109) cfu:(5 × 107-5×109) cfu:(720-880) mg:(45-55) mg:(0.009-
0.011) mg:(27-33) mg:(1.8-2.2) mg:(27-33) mg:(0.09-0.11) μ g:(0.027-0.033) ng:
(0.018-0.022) ng:(0.018-0.022) ng:(0.018-0.022) ng or 7 × 108Cfu:5 × 108Cfu:5 ×
108Cfu:800mg:50mg:0.01mg:30mg:2mg:30mg:0.1 μ g:0.03ng:0.02ng:0.02ng:0.02ng).
In the microecological microbial agent, the active constituent of the microecological microbial agent can be false single for denitrification Halomonas, production alkali
After born of the same parents bacterium and the fermented culture of Paracoccus denitrificans, or directly use or be concentrated use or the manufactured viable bacteria system through carrier adsorption
Product.
In the microecological microbial agent, the content of denitrification Halomonas can be (7 × 107-7×109) cfu/g (such as 7 ×
108cfu/g).The content of Pseudomonas alcaligenes can be (5 × 107-5×109) cfu/g (such as 5 × 108cfu/g).Paracoccus denitrificans
Content can be (5 × 107-5×109) cfu/g (such as 5 × 108cfu/g).The content of starch can be for (720-880) mg/g (such as
800mg/g).The content of ethylenediamine tetra-acetic acid can be (45-55) mg/g (such as 50mg/g).The content of magnesium sulfate can be (0.009-
0.011) mg/g (such as 0.01mg/g).The content of disodium hydrogen phosphate can be (27-33) mg/g (such as 30mg/g).The content of ferric sulfate
It can be (1.8-2.2) mg/g (such as 2mg/g).The content of potassium dihydrogen phosphate can be (27-33) mg/g (such as 30mg/g).Calcium chloride
Content can be (0.09-0.11) μ g/g (such as 0.1 μ g/g).The content of copper sulphate can be for (0.027-0.033) ng/g (such as
0.03ng/g).The content of manganese chloride can be (0.018-0.022) ng/g (such as 0.02ng/g).The content of ammonium molybdate can be
(0.018-0.022) ng/g (such as 0.02ng/g).The content of cobalt chloride can be (0.018-0.022) ng/g (such as 0.02ng/g).
Any of the above-described denitrification Halomonas concretely denitrification Halomonas (Halomonas
denitrificans)CGMCC 1.7484。
Any of the above-described Pseudomonas alcaligenes concretely Pseudomonas alcaligenes (Pseudomonas alcalophila)
CGMCC 1.8055。
Any of the above-described Paracoccus denitrificans concretely Paracoccus denitrificans (Paracoccus denitrificans)
ATCC13543。
Any of the above-described microecological microbial agent concretely following at least one microbial inoculums: 1) micro-ecological bacterial of nitrate is removed
Agent；2) microecological microbial agent of nitrate in water removal is removed；3) microecological microbial agent of nitrate in aquaculture system is removed.
The present invention also protects the method for preparing any of the above-described microecological microbial agent, can be by any of the above-described denitrification
Halomonas, Pseudomonas alcaligenes, Paracoccus denitrificans, starch, ethylenediamine tetra-acetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate,
Potassium dihydrogen phosphate, calcium chloride, copper sulphate, manganese chloride, ammonium molybdate and cobalt chloride are mixed according to the ratio, obtain the Tiny ecosystem
Microbial inoculum.
In the above-mentioned method for preparing microecological microbial agent, denitrification Halomonas, Pseudomonas alcaligenes and Paracoccus denitrificans can
First fermented and cultured prepares fermentation liquid respectively, then mixes again with other components according to the ratio.The culture of the fermented and cultured
Base can be LB liquid medium.The temperature of the fermented and cultured can be 35-39 DEG C (such as 35-37 DEG C, 37-39 DEG C, 35 DEG C, 37 DEG C
Or 39 DEG C).The time of the fermented and cultured can be 36-60h (such as 36-48h, 48-60h, 36h, 48h or 60h).
The present invention also protects the application of any of the above-described microecological microbial agent, can be a1)-a4) at least one: a1) go
Except nitrate；A2 nitrate in water removal) is removed；A3) the product of preparation removal nitrate；A4) production of nitrate in water removal is gone in preparation
Product.
In above-mentioned application, the removal nitrate or the mode for removing nitrate in water removal can be anaerobic denitrifying, good
At least one of oxygen denitrification and assimilation.
It is described that nitrate in water removal is gone concretely to remove nitrate in the water body of aquaculture in above-mentioned application.
The present invention also protects a kind of method for removing nitrate, it may include following steps: to the liquid phase body containing nitrate nitrogen
Any of the above-described microecological microbial agent is added in system, to remove the nitrate in liquid-phase system.
In the above method, the liquid phase of the liquid-phase system containing the nitrate nitrogen concretely nitrate nitrogen containing 30mg/L or more
System.
In the above method, the liquid-phase system can be the water body of aquaculture.
The present invention also protects a kind of circulating water culture system, including aquaculture pond, aerobic tank and filtering ponds；The recirculated water is supported
The system of growing may also include anoxic pond；The anoxic pond is located at the upstream in the downstream of aquaculture pond, aerobic tank.
In above-mentioned circulating water culture system, the volume of the anoxic pond can be 1/ (such as 1/3,1/ (3-5) of aquaculture pond volume
4 or 1/5).
In above-mentioned circulating water culture system, the anoxic pond can be made of at least one processing pond；Each processing pond can
Including pond body, inlet and outlet；One is located at the top of pond body side wall in inlet and outlet, another is located at pond body
The bottom of side wall.
In above-mentioned circulating water culture system, the processing pond can also be equipped with and fill for reducing the water process of hydraulic detention time
It sets.
The water treatment facilities for reducing hydraulic detention time concretely polypropylene honeycomb inclined plate or polypropylene bee
Nest inclined tube.
In above-mentioned circulating water culture system, the aerobic tank can dispose aerating system or equipped with spherical polyethylene filler (mesh
It is to keep aeration).
In above-mentioned circulating water culture system, the filtering ponds are equipped with filtering material (such as cobblestone, rubble, quartz sand).
In one embodiment of the invention, the anoxic pond is specifically made of 3 processing ponds.It is set in 3rd processing pond
Set polypropylene honeycomb inclined plate.
In another embodiment of the present invention, the anoxic pond is made of 1 processing pond, and poly- third is arranged in the processing pond
Alkene honeycomb inclined plate.
The method that the present invention also protects nitrate in removal aquaculture system, concretely S1) or S2) or S3).
S1 aquaculture) is carried out using any of the above-described circulating water culture system, any of the above-described micro- life is added to water body
State microbial inoculum removes the nitrate in water body.
S2 aquaculture) is carried out using any of the above-described circulating water culture system, is added to anoxic pond and/or aerobic tank above-mentioned
Any microecological microbial agent removes the nitrate in water body.
S3 any of the above-described microecological microbial agent) is added to the water body of aquaculture, removes the nitrate in water body.
Above-mentioned S1) in, described " any of the above-described microecological microbial agent is added to water body " is concretely when the nitre in water body
Hydrochlorate nitrogen concentration reaches 30mg/L or more, and any of the above-described microecological microbial agent is added.
Above-mentioned S2) in, described " any of the above-described microecological microbial agent is added to anoxic pond and/or aerobic tank " concretely
When the nitrate nitrogen concentration in water body reaches 30mg/L or more, to anoxic pond and/or any of the above-described micro- life of aerobic tank addition
(any of the above-described microecological microbial agent can be activated first state microbial inoculum.Activation method are as follows: 1 mass parts are any of the above-described described micro-
Ecological microbial agent and 15 mass parts water (such as tap water or river water) mixing, stand 2h or more (such as 3h)).Any of the above-described micro- life
State microbial inoculum can put into anoxic pond and aerobic tank is fifty-fifty, can also only investment anoxic pond, can also only investment aerobic tank, can also be with
The ratio of investment anoxic pond and any of the above-described microecological microbial agent of aerobic tank is determined according to anoxic pond and the volume of aerobic tank.
Above-mentioned S3) in, described " any of the above-described microecological microbial agent is added to the water body of aquaculture " concretely when
Nitrate nitrogen concentration in the water body of aquaculture reaches 30mg/L or more, and any of the above-described microecological microbial agent is added.
The input amount of any of the above-described microecological microbial agent concretely 200-600g/m3Water body (such as 200-400g/m3Water
Body, 400-500g/m3Water body, 500-600g/m3Water body, 200g/m3Water body, 250g/m3Water body, 400g/m3Water body, 500g/m3
Water body or 600g/m3Water body).
To keep the removal effect of nitrate more lasting, can at regular intervals (such as 30 days) put into it is any of the above-described as described in
Microecological microbial agent.In order to increase advantage of the bacterial strain in microbiologic population in microecological microbial agent, any of the above-described micro- life is put into
State microbial inoculum can divide 1 time or more (such as 2 times) to carry out.In one embodiment of the invention, it proceeded as follows every 30 days: first throwing
Enter any of the above-described microecological microbial agent (input amount 500g/m3Water body), 3 days and then any of the above-described micro- life of investment
State microbial inoculum (input amount 250g/m3Water body).
Test proves that microecological microbial agent prepared by the present invention can play denitrification functions in aerobic and anaerobic state, passes through
Three kinds of anaerobic denitrifying, aerobic denitrification and assimilation modes remove water body nitrate, therefore can launch and become reconciled in anoxic pond simultaneously
Oxygen pond.Operation of the present invention is simple, at low cost simultaneously, and can reduce the dense of ammonia nitrogen, nitrite etc. simultaneously in removal nitrate
Degree.It is supported it can be seen that recirculated water can be improved using circulating water culture system provided by the invention and its dedicated microecological microbial agent
The Nitrate Accumulation problem of mode is grown, and saves water source, reduce aquaculture cost, environmental sound.Present invention optimizes circulations
Water aquaculture model has important application value.
Detailed description of the invention
Fig. 1 is the water cycle process of circulating water culture system.
Fig. 2 is the change of water quality of circulating water culture system in embodiment 3.
Specific embodiment
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.
Experimental method in following embodiments is unless otherwise specified conventional method.
Test material as used in the following examples is unless otherwise specified to buy from routine biochemistry reagent shop
It arrives.
Denitrification Halomonas (Halomonas denitrificans) CGMCC 1.7484 and production in following embodiments
Pseudomonas alcaligenes (Pseudomonas alcalophila) CGMCC 1.8055 is preserved in China General Microbiological culture presevation
Administrative center (abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode 100101), the public can be from China
General Microbiological Culture preservation administrative center obtains.Hereinafter, denitrification Halomonas (Halomonas
Denitrificans) 1.7484 abbreviation denitrification Halomonas of CGMCC, Pseudomonas alcaligenes (Pseudomonas
Alcalophila) 1.8055 abbreviation Pseudomonas alcaligenes of CGMCC.
Paracoccus denitrificans (Paracoccus denitrificans) ATCC 13543 in following embodiments is preserved in beauty
State's type culture collection warehousing (abbreviation ATCC, address: American Type Culture Collection (ATCC)
10801University Boulevard Manassas, VA 20110USA), the public can be from American Type Culture collection warehousing
Obtain the bacterial strain.Hereinafter, 13543 abbreviation denitrogenation pair of Paracoccus denitrificans (Paracoccus denitrificans) ATCC
Coccus.
The preparation of embodiment 1, microecological microbial agent
One, the acquisition of fermentation liquid
1, the acquisition of denitrification Halomonas fermentation liquid
(1) 0.1mL denitrification Halomonas preservation liquid is inoculated in 200mLLB fluid nutrient medium, 37 DEG C, 200rpm oscillation
48h is cultivated, seed liquor is obtained.
(2) after completing step (1), the seed liquor is all inoculated in 40L LB liquid medium, 37 DEG C, 200rpm vibration
Culture 48h is swung, denitrification Halomonas fermentation liquid is obtained.
2, the acquisition of Pseudomonas alcaligenes fermentation liquid
(1) 0.1mL Pseudomonas alcaligenes preservation liquid is inoculated in 200mLLB fluid nutrient medium, 37 DEG C, 200rpm oscillation training
48h is supported, seed liquor is obtained.
(2) after completing step (1), the seed liquor is all inoculated in 40L LB liquid medium, 37 DEG C, 200rpm vibration
Culture 48h is swung, Pseudomonas alcaligenes fermentation liquid is obtained.
3, the acquisition of Paracoccus denitrificans fermentation liquid
(1) 0.1mL Paracoccus denitrificans preservation liquid is inoculated in 200mLLB fluid nutrient medium, 37 DEG C, 200rpm shaken cultivation
48h obtains seed liquor.
(2) after completing step (1), the seed liquor is all inoculated in 40L LB liquid medium, 37 DEG C, 200rpm vibration
Culture 48h is swung, Paracoccus denitrificans fermentation liquid is obtained.
Two, the preparation of microecological microbial agent
Denitrification Halomonas fermentation liquid, the Pseudomonas alcaligenes fermentation liquid, Paracoccus denitrificans fermentation that step 1 is obtained
Liquid and nutrient mixed powder are (by starch, ethylenediamine tetra-acetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, chlorine
Change calcium, copper sulphate, manganese chloride, ammonium molybdate and cobalt chloride composition) mixing, obtain microecological microbial agent.In microecological microbial agent, denitrification
The content of Halomonas is 7 × 108Cfu/g, the content of Pseudomonas alcaligenes are 5 × 108The content of cfu/g, Paracoccus denitrificans is
5×108Cfu/g, the content of starch are 800mg/g, and the content of ethylenediamine tetra-acetic acid is 50mg/g, and the content of magnesium sulfate is
0.01mg/g, the content of disodium hydrogen phosphate are 30mg/g, and the content of ferric sulfate is 2mg/g, and the content of potassium dihydrogen phosphate is 30mg/
G, the content of calcium chloride are 0.1 μ g/g, and the content of copper sulphate is 0.03ng/g, and the content of manganese chloride is 0.02ng/g, ammonium molybdate
Content be 0.02ng/g, the content of cobalt chloride is 0.02ng/g.
It should be noted that the content of beary metal in microecological microbial agent is low, mainly microorganism absorbs, and not will lead to cultivation
The heavy metals exceeding standard of object.
The use of embodiment 2, the foundation of circulating water culture system and microecological microbial agent in the circulating water culture system
One, the foundation of circulating water culture system
In traditional circulating water culture system, the water of aquaculture pond enters solid-liquid separation system, filters subsequently into aeration and biological
Pond, without independent specific anoxic zone.The present inventor passes through many experiments, establishes recirculated water as shown in Figure 1 and supports
Grow system (water cycle process are as follows: aquaculture pond is drained into anoxic pond, and anoxic pond is drained into aerobic tank, and aerobic tank is drained into
Filtering ponds, filtering ponds draining to aquaculture pond).Compared with traditional circulating water culture system, circulating water cultivation that the present invention establishes
System mainly sets up anoxic pond in aquaculture pond downstream, aerobic tank upstream；By detect water body quality timing or not timing to
The microecological microbial agent of the preparation of embodiment 1 is added in anoxic pond.Setting up for anoxic pond can reach two purposes, first is that solid conducive to water body
The precipitating of body pollution object, second is that making the carry out ammonification of the itrogenous organic substance in water body.
Anoxic pond volume is the 1/4 of aquaculture pond volume, positioned at the downstream of aquaculture pond, the upstream of aerobic tank.Specific structure is such as
Under: anoxic pond is respectively processing pond 1, processing pond 2 and processing pond 3 from upstream to downstream；The tank volume of processing pond 1 is anoxic pond
The 1/10 of volume, the top of the pond body side wall adjacent with aquaculture pond of processing pond 1 are provided with water inlet, the bottom of another pond body side wall
It is provided with water outlet (water inlet that the water outlet is also processing pond 2)；The tank volume of processing pond 2 is the 4/ of anoxic pond volume
10, the top of the pond body side wall adjacent with processing pond 3 of processing pond 2 is provided with water outlet, and (water outlet is also the water inlet of processing pond 3
Mouthful)；The tank volume of processing pond 3 is the 5/10 of anoxic pond volume, and the bottom of the pond body side wall adjacent with aerobic tank of processing pond 3 is set
It is equipped with water outlet.Polypropylene honeycomb inclined plate is also set up in processing pond 3, for shortening the sedimentation time, waterpower needed for reducing processing pond
Residence time.
When operation, the draining of aquaculture pond flows into processing pond 1 by water inlet, is handled by the water outlet inflow of processing pond 1
Pond 2, flows into processing pond 3 by the water outlet of processing pond 2, flows into aerobic tank by the water outlet of processing pond 3.
Being provided with conducive to pollutants precipitatings such as fish excrement, residual feedings for multiple processing ponds of anoxic pond, provides multiplicity for microorganism
Environment.Wherein first processing pond has been mainly buffering transitional function, and subsequent processing pond is then formed and oxygen-micro-environmental hypoxia,
Pollutant is decomposed to form ammonia nitrogen, the lesser substance of short chain organic acid equimolecular quantity under microecological microbial agent effect.
Two, the use in the circulating water culture system that microecological microbial agent step 1 is established
1 mass parts microecological microbial agent is taken, 15 mass parts tap water or river water is added, 3h (purpose is activation) is stood, obtains
To activating solution.
The application method in circulating water culture system that microecological microbial agent step 1 is established is as follows:
1, the ammonia nitrogen concentration for the circulating water culture system established when step 1 is 2mg/L or less, nitrite nitrogen concentration is
1mg/L or less and nitrate nitrogen concentration put into activating solution in anoxic pond and aerobic tank, input amount is in 30mg/L or more respectively
500g microecological microbial agent/m3Breeding water body.
2, it completes the 3rd day after step 1, puts into activating solution respectively in anoxic pond and aerobic tank, input amount is the micro- life of 250g
State microbial inoculum/m3Breeding water body.
The purpose for adding microecological microbial agent is to increase advantage of the bacterial strain in microbiologic population in microecological microbial agent, to make
The removal effect of nitrate becomes apparent from also more longlasting.
3, complete step 1 after the 5th day, can it is appropriate remove anoxic pond and aerobic tank sludge, be conducive to recirculated water support
Grow the improvement of system water quality.
The application for the circulating water culture system that microecological microbial agent and embodiment 2 prepared by embodiment 3, embodiment 1 is established
1 mass parts microecological microbial agent is taken, 15 mass parts tap water or river water is added, 3h (purpose is activation) is stood, obtains
To activating solution.
One, using one
The present inventor establishes circulating water cultivation as described in example 2 in Longgang district Shenzhen city Hua Da agricultural laboratories
System.The circulating water culture system is made of culture zone and water treatment zone.Culture zone is made of two cylindrical aquaculture ponds, often
The volume of a aquaculture pond is 1m3.Water treatment zone is by 0.5m3Anoxic pond, 1m3Aerobic tank and 0.5m3Filtering ponds composition.It lacks
Oxygen pond is divided into three lattice, is corresponding in turn to processing pond 1, processing pond 2 and processing pond 3, and polypropylene honeycomb inclined plate is arranged in processing pond 3.It is aerobic
Pond is equipped with spherical polyethylene filler, keeps aeration.Filtering ponds place the filtering materials such as cobblestone, rubble.
1, it tests the 1st day, the Tilapia mossambica that investment weighs about 30g/ tail is cultivated.Cultivation density is 10kg/m3.Each cultivation
It is 300g/ days that forage volume is launched in pond.Aquaculture pond dissolved oxygen amount is 2-2.5mg/L.
2, it tests the 40th day, observes water body situation.The result shows that the solid waste such as the fish excrement of culture zone, residual feeding largely accumulate
Gather in anoxic pond, form of gathering is precipitating sludge and floating sludge；The water body of aerobic tank and filtering ponds is limpider.
3, during testing, ammonia nitrogen concentration, nitrite nitrogen concentration and the nitrate nitrogen concentration of aquaculture pond draining are detected respectively
(ammonia nitrogen, nitrite nitrogen, nitrate nitrogen detection method respectively according to Chinese environmental protection portion promulgate HJ535-2009, GB7493-
87 and GB7480-87 standard), and handled according to nitrate nitrogen concentration.
Test to 49 days: successively there is peak in the ammonia nitrogen concentration and nitrite nitrogen concentration of aquaculture pond draining.
Test the 50-70 days: the water quality of aquaculture pond draining starts to stablize, and ammonia nitrogen concentration is between 0-3mg/L, nitrite
Nitrogen concentration is substantially zeroed, nitrate nitrogen concentration persistent accumulation.
Test the 71st day: the ammonia nitrogen concentration of aquaculture pond draining is 1.2mg/L, and nitrite nitrogen concentration is 0.2mg/L, nitric acid
Salt nitrogen concentration is 49.2mg/L.Since nitrate nitrogen concentration is higher, start to add at the microecological microbial agent of the preparation of embodiment 1
Reason.Specific processing mode are as follows: add 8kg activating solution to anoxic pond, 8kg activating solution is added to aerobic tank.
Test the 72nd day: the nitrate nitrogen concentration of aquaculture pond draining is 31.8mg/L (than the 71st day reduction 17.4mg/ of test
L), ammonia nitrogen concentration is 2.0mg/L (slightly rising than test the 71st day), and nitrite nitrogen concentration is 1.2mg/L (than test the 71st
It slightly rises).
Test the 73rd day and the 74th day: the nitrate nitrogen concentration of aquaculture pond draining is respectively 33.5mg/L and 36.3mg/L
(nitrate nitrogen accumulating rate and add almost the same before microecological microbial agent), ammonia nitrogen concentration are respectively 0.6mg/L and 1.9mg/L,
Nitrite nitrogen concentration is 0.2mg/L.It can be seen that water quality is not caused obviously to deteriorate and nitre after adding microecological microbial agent
Hydrochlorate quicks rebound.
It tests the 74th day: adding microecological microbial agent and handled.Specific processing mode are as follows: add 4kg activating solution to scarce
Oxygen pond, 4kg activating solution are added to aerobic tank.
Test the 75th day: the nitrate nitrogen concentration of aquaculture pond draining is 28.3mg/L, ammonia nitrogen concentration 0.8mg/L, nitrous
Hydrochlorate nitrogen concentration is 0.9mg/L.
Later every 30 days (as test the 101st day, test the 131st day and test the 161st day, by 8kg activating solution add to
Anoxic pond, 8kg activating solution are added to aerobic tank；The 104th day, test the 134th day and test the 164th day are tested, by 4kg activating solution
Add to anoxic pond, 4kg activating solution is added to aerobic tank) microecological microbial agent is added according to the method described above, and detect aquaculture pond row
Ammonia nitrogen concentration, nitrite nitrogen concentration and the nitrate nitrogen concentration of water.
The change of water quality of aquaculture pond draining is shown in Fig. 2: nitrate concentration declines after adding microecological microbial agent.Do not add Tiny ecosystem
The nitrate of microbial inoculum is then persistent accumulation.The result shows that add nitrate concentration after microecological microbial agent obtain it is a degree of
Control.
Detect the indexs such as the content of beary metal of Tilapia mossambica and growth, breeding, metabolism in whole process.The result shows that Rofe
The content of beary metal of fish is not exceeded, and the indexs such as growth, breeding, metabolism are completely normal, with the recirculated water for not adding microecological microbial agent
The Tilapia mossambica of cultivating system cultivation is compared without significant difference.
Two, using two
The present inventor establishes circulating water culture system as described in example 2 in Shenzhen roc new district.The circulation
Water cultivating system is the rectangle cement pit of a 3m × 5m × 0.6m (respectively corresponding length, width and height), and wherein half is as culture zone
(4.5m3), half is as water treatment zone (4.5m3)。
Water process region is equally divided into anoxic zone (1.5m with partition3), aerobic zone (1.5m3), filtering area (1.5m3).Anoxic
Polypropylene honeycomb inclined plate is arranged in area.Aerobic zone disposes aerating system, and dissolved oxygen is between 2-3mg/L.Filtering area launches quartz sand.
Hole in partition, the water body of culturing area flow through anoxic zone, aerobic zone and filtering area in order, extract filtering area water with water pump
Body adds to culturing area.
1, it tests the 1st day, the fancy carp that investment weighs about 96g/ tail is cultivated.Cultivation density is 5kg/4.5m3(i.e. culture zone
Put into 5kg fancy carp).It is 150g/ days that forage volume is launched in culture zone.
2, test the 80th day: the nitrate nitrogen concentration of water body is 75.4mg/L.Since nitrate nitrogen concentration is higher, start to throw
The microecological microbial agent for adding embodiment 1 to prepare is handled.Specific processing mode are as follows: 24kg activating solution is added to anoxic pond,
24kg activating solution is added to aerobic tank.
3, test the 81st day, the 82nd day and the 83rd day: the nitrate nitrogen concentration of water body is respectively 50.3mg/L, 52.7mg/L
And 53.9mg/L.
4, it tests the 83rd day: adding microecological microbial agent and handled.Specific processing mode are as follows: by 12kg activating solution add to
Anoxic pond, 12kg activating solution are added to aerobic tank.
5, test the 84th day, the 85th day and the 86th day: the nitrate nitrogen concentration of water body is respectively 38.4mg/L, 40.5mg/L
And 41.7mg/L, ammonia nitrogen concentration and nitrite concentration are between 0-2mg/L.
The result shows that fancy carp survival rate reaches 96%, water body nitrate nitrogen concentration is substantially reduced.
Detect the indexs such as the content of beary metal of fancy carp and growth, breeding, metabolism in whole process.The result shows that fancy carp
Content of beary metal is not exceeded, and the indexs such as growth, breeding, metabolism are completely normal, with the circulating water cultivation for not adding microecological microbial agent
The fancy carp of system cultivation is compared without significant difference.
Claims (10)
1. a kind of microecological microbial agent comprising Paracoccus denitrificans.
2. microecological microbial agent as described in claim 1, it is characterised in that: the microecological microbial agent further includes denitrification salt unit cell
Bacterium and Pseudomonas alcaligenes.
3. microecological microbial agent as claimed in claim 1 or 2, it is characterised in that: the microecological microbial agent further includes for maintaining
The nutrient of bacterial growth.
4. microecological microbial agent as claimed in claim 3, it is characterised in that: described for maintaining the nutrient packet of bacterial growth
Include starch, ethylenediamine tetra-acetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, potassium dihydrogen phosphate, calcium chloride, copper sulphate, manganese chloride,
Ammonium molybdate and cobalt chloride.
5. microecological microbial agent as claimed in claim 4, it is characterised in that: in the microecological microbial agent, denitrification Halomonas,
Pseudomonas alcaligenes, Paracoccus denitrificans, starch, ethylenediamine tetra-acetic acid, magnesium sulfate, disodium hydrogen phosphate, ferric sulfate, biphosphate
Potassium, calcium chloride, copper sulphate, manganese chloride, ammonium molybdate and cobalt chloride ratio be (7 × 107-7×109) cfu:(5 × 107-5×
109) cfu:(5 × 107-5×109) cfu:(720-880) mg:(45-55) mg:(0.009-0.011) mg:(27-33) mg:
(1.8-2.2) mg:(27-33) mg:(0.09-0.11) μ g:(0.027-0.033) ng:(0.018-0.022) ng:(0.018-
0.022) ng:(0.018-0.022) ng.
Be a1 6. the application of any microecological microbial agent of claim 1 to 5)-a4) at least one: a1) removal nitrate；
A2 nitrate in water removal) is removed；A3) the product of preparation removal nitrate；A4) product of nitrate in water removal is removed in preparation.
7. a kind of method for removing nitrate includes the following steps: that claim 1 is added into the liquid-phase system containing nitrate nitrogen
To 5 any microecological microbial agents, the nitrate in liquid-phase system is removed.
8. a kind of circulating water culture system, including aquaculture pond, aerobic tank and filtering ponds, it is characterised in that: the circulating water cultivation
System further includes anoxic pond；The anoxic pond is located at the upstream in the downstream of aquaculture pond, aerobic tank.
9. circulating water culture system as claimed in claim 8, it is characterised in that: the anoxic pond is by least one processing pond group
At；Each processing pond includes pond body, inlet and outlet；One is located at the upper of pond body side wall in inlet and outlet
Portion, another is located at the bottom of pond body side wall.
10. remove aquaculture system in nitrate method, be S1) S2) or S3):
S1 aquaculture) is carried out using the circulating water culture system of claim 8 or 9, claim 1 to 5 is added to water body
Any microecological microbial agent removes the nitrate in water body；
S2 aquaculture) is carried out using the circulating water culture system of claim 8 or 9, is added to anoxic pond and/or aerobic tank
Any microecological microbial agent of claim 1 to 5 removes the nitrate in water body；
S3 any microecological microbial agent of claim 1 to 5) is added to the water body of aquaculture, removes the nitrate in water body.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071141A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Denitrified microbial agent as well as preparation method and application thereof |
| CN102911897A (en) * | 2012-10-11 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Cultural method for paracoccus denitrificans and application of same to purifying aquaculture water |
| CN203194328U (en) * | 2013-03-13 | 2013-09-18 | 福州开发区三水环保科技有限公司 | Integrated circulating water aquaculture purifier |
-
2018
- 2018-11-29 CN CN201811442256.2A patent/CN109609400B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071141A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Denitrified microbial agent as well as preparation method and application thereof |
| CN102911897A (en) * | 2012-10-11 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Cultural method for paracoccus denitrificans and application of same to purifying aquaculture water |
| CN203194328U (en) * | 2013-03-13 | 2013-09-18 | 福州开发区三水环保科技有限公司 | Integrated circulating water aquaculture purifier |
Non-Patent Citations (2)
| Title |
|---|
| HIROAKI UEMOTO ET AL.: "Closed water recirculating system for fish rearing equipped with bioreactor capable of simultaneous nitrification and denitrification", 《BIOLOGICAL SCIENCES IN SPACE》 * |
| 陈进斌 等: "好氧反硝化复合菌剂的研制及其在海水养殖废水处理中的应用", 《湖北农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115093021A (en) * | 2022-07-28 | 2022-09-23 | 宁波水思清环境科技有限公司 | Sewage treatment agent and preparation method thereof |
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