CN103373767B - Method for biologically denitrifying high-salinity sewage generated in production process of catalysts - Google Patents
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Abstract
The invention discloses a method for biologically denitrifying high-salinity sewage generated in the production process of catalysts. The method is implemented by adding nitrobacteria and denitrifiers with nitrites as electron acceptors to sewage, wherein the high-salinity sewage treatment temperature is 18-40 DEG C; the dissolved oxygen is 0.2-3mg/L; and the pH value is 7.5-8.5. The method adopts autotrophic bacteria and heterotrophic bacteria which are subjected to combination of superiority to serve as reinforced microorganisms for treating sewage, adopts different addition modes, can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and truly achieves biological denitrification of the high-salinity sewage generated in the production process of catalysts by being combined with high-efficiency devices.
Description
Technical field
The invention belongs to environmental engineering dirty water living creature processing technique field, be specifically related to a kind of high biological denitrification method containing salt catalyst sewage.
Background technology
In the Catalyst Production process of petrochemical industry, ammonium salt and ammoniacal liquor are used in many places, therefore in the containing wastewater from catalyst of discharge, contain a large amount of ammonia nitrogens.Although the ammonia nitrogen in waste water can adopt the physico-chemical processes such as stripping stripping, ion-exchange, chemical oxidation to process, there is by product secondary pollution and the problem such as processing efficiency is low in these methods.By contrast, biological process is the better method of controlling water body ammonia and nitrogen pollution.
Traditional biological method is as the terminal technology of conventional sewage disposal, limited in one's ability when processing high ammonia nitrogen, low COD waste water, usually sacrifices load to realizing the qualified discharge of ammonia nitrogen pollutant in waste water.When particularly salt concn is higher in sewage, can suppress microbial growth and reduce microbic activity, to biological treatment, bringing certain difficulty.Although all carried out repeatedly a large amount of improvement from aspects such as technique and sewage treatment structures, in sewage treatment process, play certain effect, but owing to being responsible for the main body active sludge of ammonia nitrogen removal, do not change, so the removal effect of ammonia nitrogen is not still very desirable.The ammonia nitrogen problem that exceeds standard directly has influence on the comprehensive up to standard and normal production of Catalyst Production enterprise wastewater, becomes the bottleneck of restriction enterprise development, Ammonia Wastewater Treatment is become to the primary environmental issue of Catalyst Production enterprise.Therefore researching and developing economic, practical, the safe treatment technology containing ammonia nitrogen in salt catalyst wastewater becomes current study hotspot, to protection of the environment, promote the well-being of mankind significant.
No matter be traditional or novel bio-denitrification technology, increasing biomass is one of effective means improving sewage treating efficiency.Along with some novel, effective denitrification microorganisms, as allotrophic nitrobacteria, aerobic denitrifying bacteria, anaerobic ammonium oxidizing bacteria etc., separated evaluation is out in succession, biological reinforcing technology is widely applied, and people have carried out a large amount of research work to bacteria agent.
CN101302485A discloses a kind of heterotrophic nitrification microbial preparation, its cultural method and purposes, this microbial inoculum contain germ oligotrophy unit cell (
stenotrophomonas maltophiliastraindN 1.1) and pseudomonas putida (
pseudomonas putida straindN 1.2), ammonia nitrogen and the total nitrogen of this microbial inoculum in can effective elimination water body can also be removed the COD in organic waste water simultaneously, is applicable to high-concentration culture waste water and processes.This microbial inoculum is when processing the piggery wastewater that ammonia nitrogen concentration is 455 ~ 600mg/L, and experiment moves to 94 ~ 95h, and the clearance of ammonia nitrogen in waste water is reached to 87% ~ 88%, and processing water outlet ammonia-nitrogen content is 59 ~ 72mg/L; After processing 95h, the total nitrogen of water inlet 790mg/L can be processed to 164mg/L, nitrogen removal rate is 79.2%.CN200910021020.7 discloses a kind of preparation method who falls the water quality modifying microecological preparation of ammonia nitrogen and nitrous acid nitrogen; in this microbial inoculum, relate to a strain Arthrobacter CGMCC No.1282, but the probiotics of this invention belongs to aquaculture technology and ecological environmental protection technical field.In the denitrogenation processing of the high saline sewage that mentioned microorganism microbial inoculum produces in Catalyst Production process, result of use is limited, need to develop suitable denitrogenation microbial inoculum and method of wastewater treatment for high saliferous Catalyst Production sewage.
Summary of the invention
Although there is more biotechnological formulation on market, be all not suitable for processing the saliferous ammonia-containing water producing in Catalyst Production process.For the deficiencies in the prior art, the invention discloses a kind of bioremediation of Catalyst Production saliferous ammonia-containing water, the method is to using the nitrobacteria denitrification microorganism microbial inoculum close with growth conditions as biological body, using Sewage Plant active sludge as rear salinity, ammonia nitrogen and a small amount of metal ion reducing in sewage of microbe carrier combination, solve the saliferous ammonia-containing water that produces in existing Catalyst Production process and process problem, make the sewage after processing can qualified discharge.
The present invention is high containing in the biological denitrification method of salt catalyst sewage, and sewage quality is characterized as: general 30 ~ 600 mg/L of ammonia nitrogen concentration, COD(Cr method, as follows) concentration is 30 ~ 1000mg/L, specific conductivity 5 ~ 60ms/cm, also contains the Cu of 2 ~ 50mg/L
2+, 2 ~ 100mg/L Zn
2+, SS(suspended substance) and be 10 ~ 1000mg/L, pH6 ~ 10.Unloading phase operating process as follows: the temperature of sewage disposal is 18-40 ℃, and dissolved oxygen is 0.1 ~ 4 mg/L, is preferably 0.2 ~ 3mg/L, and pH is 7.0-9.0, is preferably 7.5-8.5; Using sludge sewage as microbe carrier, activated sludge concentration lower than 4000mg/L condition under preferably activated sludge concentration be to add nitrobacteria under 1000～3000mg/L condition, in system nitrite nitrogen account for total nitration product more than 50% be preferably 50%～80% time add denitrification microbial inoculum, when ammonia nitrogen and nitrate are all lower than 50 mg/L during preferably lower than 25mg/L, unloading phase, finishes, and enters steady running operation.The denitrification microbial inoculum that the inventive method is used contain Staphylococcus cohnis (
staphylococcus cohnii) FSDN-C, and
arthrobacter creatinolyticusfDN-1 and
flavobacterium mizutaiiat least one in FDN-2.Staphylococcus cohnis (
staphylococcus cohnii) FSDN-C on July 14th, 2011 be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcodes 100101), deposit number is CGMCC NO.5062.
arthrobacter creatinolyticusfDN-1 on March 11st, 2010 be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcodes 100101), deposit number is CGMCC NO.3657.
flavobacterium mizutaiifDN-2 on March 11st, 2010 be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcodes 100101), deposit number is CGMCC NO.3659.
In the inventive method, sludge sewage can be from oil refining sewerage treatment plant or catalyzer sewage work or municipal wastewater treatment plant etc.
In the inventive method, the nitrobacteria adding comprises Nitromonas and Nitrosomas, be preferably nitrosification dominant microflora, as Nitrosomas accounts for more than 60% nitrobacteria of nitrifier total quantity, guarantee that the nitrite nitrogen of reaction system accounts for the more than 50% of total nitration product.Nitrobacteria can adopt various existing schemes to cultivate, and also can adopt commercially available microbial inoculum, as the nitrosification dominant microflora that can obtain according to CN201010221166.8.
The inventive method to newly-started system first to the active sludge of throwing in sewage work in sewage treatment structure, active sludge volumetric usage for by 3000～15000mg/L preferably 4000～10000mg/L add.After the vexed suitable time that exposes to the sun, add nitrobacteria, in the vexed process of exposing to the sun, DO is controlled at 3 ~ 5mg/L, general vexed exposing to the sun 24 ~ 168 hours.For the system of moving, can be by sludge concentration in regulation system to lower than 5000mg/L, be preferably under the condition of 3000～5000 mg/L and add nitrobacteria.
In the inventive method, nitrobacteria dosage is that 100 ~ 1000mg/L adds according to MLSS.Add after nitrobacteria, in system, nitrite nitrogen accounts for 50% of total nitration product and can add denitrification microbial inoculum when above.
Nitrobacteria can disposablely add also and can add in batches, when in waste water, ammonia nitrogen concentration is lower than 50mg/L, can stop adding.Denitrification microbial inoculum need add in batches, preferably divides and adds for 3 ~ 10 times, every 1～7 day, adds once.By the volume ratio that accounts for the amount of disposing of sewage 0.01% ~ 1% per hour, add first, successively successively decrease, each dosage successively decreases 30%～70% than last time later, when nitrate stops adding during lower than 25mg/L.Two kinds of thalline also can intersect and add, add thalline after Sewage treatment systems can not spoil disposal in 3 months.For periodical operation technique, the amount of disposing of sewage per hour is average treatment amount.
In the denitrification microbial inoculum that the inventive method is used, Staphylococcus cohnis FSDN-C colony colour is white, and bacterial strain individuality is spherical, and without gemma, gramstaining is positive, and catalase is positive, and oxidase negative, can utilize several kinds of carbon source.Arthrobacter FDN-1 colony colour is yellow, bacterial strain is individual is bar-shaped, without gemma, can move; Gramstaining is positive, and catalase is positive, and oxidase negative, can utilize several kinds of carbon source.Shui Shi Flavobacterium FDN-2 colony colour is white, bacterial strain is individual is shaft-like, without gemma; Gramstaining is negative, and catalase is positive, and oxidase positive, can utilize several kinds of carbon source.
In the denitrification microbial inoculum that the inventive method is used, Staphylococcus cohnis FSDN-C, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 all can be take nitrite nitrogen and be carried out denitrification denitrogenation as nitrogenous source under aerobic and anaerobic environment, in denitrogenation, can remove COD.Wherein Staphylococcus cohnis FSDN-C can utilize several kinds of carbon source, has certain salt resistant character, can process high saline sewage.
In the denitrification microbial inoculum that the inventive method is used, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 can be wherein a kind of, also can be the mixing thalline of two kinds of arbitrary proportions, preferably the thalline volume ratio of Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 be 1:0.1 ~ 3." Staphylococcus cohnis FSDN-C " is 1:0.1 ~ 10 with the ratio of " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " two bacteroids, is preferably 1:0.2 ~ 5.(press thalline volumeter, thalline volume turns at per minute 10,000 the thalline volume that under condition, centrifugation obtained after 5 minutes after cultivating, lower same).In denitrification microbial inoculum of the present invention, can contain suitable additive, as nutritive substance, preservation auxiliary agent etc., concrete additive types and consumption are well known to those skilled in the art.In denitrification microbial inoculum, the volume sum of above-mentioned thalline accounts for 10%～60% of denitrification microbial inoculum cumulative volume, is preferably 20%～50%.
A kind of concrete preparation method of the microorganism denitrification microbial inoculum that the inventive method is used comprises following content:
1, Staphylococcus cohnis FSDN-C of the present invention, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are inoculated on solid medium and are activated respectively;
2, with connecing collarium, scrape a ring bacterium liquid and be inoculated in beef extract-peptone liquid medium, under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition, concussion is cultivated 1 ~ 3 day to logarithmic phase, obtains liquid bacterial agent seed liquor;
3, thalline, the concentrated microbial inoculum that is the inventive method use will be collected after above-mentioned seed liquor amplification culture.
Microbiobacterial agent amplification culture of the present invention nutrient solution used can be autogamy or actual in nitrite nitrogen waste water, and the nitrite nitrogen concentration in nutrient solution is 100mg/L ~ 1000mg/L, carbon-nitrogen mass ratio 2:1 ~ 10:1; Culture condition is 15 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is lower than 1.0mg/L.
The present invention's amplification culture reactor used can be various suitable structures, has good aeration and stirring system.In culturing process, pH value does not need to regulate.
In the inventive method; the microbiobacterial agent using is liquid microbial inoculum or solid-state microbial inoculum; after the concentration of above-mentioned amplification culture, obtain the bacteria suspension that concentration is higher; after being added to nutritive medium or nutritive medium and protectant mixed solution, these bacteria suspensions can be prepared into liquid microbial inoculum; liquid microbial inoculum is generally 10～20g/L(MLSS in the concentration of MLSS and represents suspended sediment concentration), also can utilize the existing method in this area to be prepared into the microbial inoculum of dry powder.Can adopt resistance to storage, container or the equipment antifreeze, that be convenient to the materials such as transportation such as plastics bag, Plastic Bottle or plastic tank to pack.Packaging Method adopts the existing routine techniques in this area, and Packing Unit is that the microbial inoculum after 50 ~ 5000g/ bag (bag or bottle) packing can be preserved as the case may be under room temperature or cold condition.The room temperature preservation quality guaranteed period is 1 ~ 6 month, and the cryopreservation quality guaranteed period is 1 ~ 5 year, and after preserving, activity decreased rate is less than 10%.
The present invention's concentration method used can not affect by centrifugal or filtration etc. the method for microbial activity.
First microbial inoculum preparation process of the present invention carries out thalline activation; Then carry out seed liquor cultivation, finally will after seed liquor amplification culture, collect thalline, concentrate, pack and save backup.The microbiobacterial agent tolerance and the strong adaptability that obtain, good impact resistance, the removal load of total nitrogen is high, treatment effect good.
The catalyzer saliferous that the present invention proposes, containing the treatment process of ammonia sewage, is mainly that the optimum combination by directly adding microorganism in nitrobacteria and denitrification microbial inoculum, reinforcement sludge, the group status of change denitrification microorganism are realized.Substrate is worked in coordination, competed mutually to described different microorganism, effect when ammonia nitrogen and TN removel rate are used separately higher than any microorganism, particularly supplement and can utilize after the Staphylococcus cohnis FSDN-C of several kinds of carbon source, strengthen the salt resistant character of Sewage treatment systems, improved adsorptivity and the flocculence of mud.Because so population effect is strong, wide to the scope of application of waste water quality to the withstand shock ability of waste water, when removing the pollutents such as ammonia nitrogen and COD, be conducive to the removal of salinity and metal ion, and can effectively reduce the turbidity of water outlet, obviously improve the treatment effect of sewage.
Embodiment
The nitrobacteria that embodiment is used cultivates by CN201010221166.8 embodiment 1 method the nitrosification dominant microflora obtaining.Further illustrate by the following examples process and the effect of the inventive method.
the preparation of embodiment 1 denitrification microbial inoculum
1, Staphylococcus cohnis FSDN-C of the present invention, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 being inoculated in respectively on beef extract-peptone solid medium is to activate in 30 ℃ of constant incubators in temperature, culture medium prescription is: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L, add 2.0% agar, wherein in Staphylococcus cohnis FSDN-C solid medium, add 0.5mL/L methyl alcohol.The culture condition of bacterial strain is for being that 30 ~ 35 ℃, 150 ~ 240rpm concussion are cultured to logarithmic phase and can gather in the crops thalline for the preparation of microbiobacterial agent in temperature.
2, with connecing collarium, scrape a ring solid colony inoculation in beef extract-peptone liquid medium, under 25 ~ 35 ℃ of temperature, 150 ~ 240rpm aerobic condition, concussion is cultivated 1 ~ 3 day to logarithmic phase, obtains liquid bacterial agent seed liquor; Culture medium prescription is: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L, wherein adds 0.5mL/L methyl alcohol in Staphylococcus cohnis FSDN-C substratum.
3, above-mentioned Staphylococcus cohnis FSDN-C, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 seed liquor are carried out respectively to amplification culture according to 3:2:1,1:1:1 and the combination of 1:2:3 ratio in having the reactor of good stirring system, nitrite nitrogen concentration in nutrient solution is 200mg/L ~ 800mg/L, carbon-nitrogen mass ratio 2:1 ~ 10:1; Culture condition is 25 ℃～35 ℃ of temperature; PH6.5～10.0; Dissolved oxygen is lower than 1.0mg/L.
To the liquid bacterial suspension A (seed liquor blending ratio 3:2:1), the B(seed liquor blending ratio 1:1:1 that obtain through amplification culture) and C(seed liquor blending ratio 1:2:3) collect and concentrate, then add the nutritive medium of bacteria suspension two volumes.NO in every liter of nutritive medium
2 -, Fe
2+, Mg
2+, K
+, Ca
2+these five kinds of cationic mole of allocation ratios are 2000:5:20:20:15, wherein NO
2 -mass concentration be 500mg/L, obtain denitrification microbial inoculum A, denitrification microbial inoculum B and denitrification microbial inoculum C, in denitrification microbial inoculum, the volume of thalline accounts for 30%.
embodiment 2
The high ammonia nitrogen containing wastewater from catalyst of certain chemical plant saliferous, waste water quality is characterized as ammonia nitrogen concentration 400 ~ 600 mg/L, and COD concentration is 200 ~ 500mg/L, pH8.87, specific conductivity 35 ~ 40ms/cm, Cu
2+concentration is that 2 ~ 10mg/L, concentration are Zn
2+10 ~ 20mg/L.Adopt SBR art breading, while going into operation, first using the active sludge of oil refining sewerage factory as kind of a mud, after throwing in, MLSS is 3800mg/L, at DO, is after vexed quick-fried 7 days, to start to add nitrobacteria under 2 ~ 5mg/L condition, is that 600mg/L adds first according to MLSS, by MLSS, be that 200mg/L adds again two days later, two days later, DO is 2 ~ 4mg/L in operation, and pH value is 7.8, intrasystem nitrite nitrogen accounts for 50% of total nitration product, now adds the denitrification microbial inoculum A of embodiment 1.Every 5 days, add once, by the volume ratio that accounts for the amount of disposing of sewage 0.1% per hour, add first, successively successively decrease, each dosage successively decreases 30% than last time later, move after 20 days water outlet ammonia nitrogen concentration lower than 25mg/L, nitrate is lower than 20mg/L, and two kinds of thalline all stop adding.Continue operation for some time, water outlet ammonia nitrogen and total nitrogen concentration are all stable, and lower than 15mg/L, COD concentration is lower than 50mg/L, and specific conductivity is lower than 1500 μ s/cm, Cu
2+concentration is lower than 0.5mg/L, Zn
2+concentration, lower than 1.0mg/L, has realized the up to standard processing of saliferous containing ammonia sewage.
embodiment 3
The existing sewage quality of certain catalyst plant is: ammonia nitrogen concentration 50 ~ 300 mg/L, COD concentration is 50 ~ 500mg/L, pH8.93, specific conductivity 25 ~ 35ms/cm, Cu
2+concentration is 5 ~ 10mg/L, Zn
2+concentration is 15 ~ 25mg/L, also contains the nitrate of 400mg/L left and right simultaneously.Impact due to metal ions such as high salt concentration and copper zinc, causes sewage can not realize processing up to standard.Adopting the inventive method to process, when the MLSS in original system is 4000mg/L, add nitrobacteria, is that 600mg/L adds according to MLSS first, moves one week rear water outlet ammonia nitrogen concentration lower than 25mg/L, can stop adding nitrobacteria.Continue operation after one week nitrous rate be 60%, now starting according to dosage is first the denitrification microbial inoculum B that the volume ratio that accounts for the amount of disposing of sewage 0.5% per hour adds embodiment 1, adds once, dosage successively decreases 50% than last time at every turn every 3 days.Move that after one month, water outlet ammonia nitrogen and total nitrogen concentration are all lower than 25mg/L, in system, sludge character is good, has realized the effective processing of saliferous containing ammonia catalyzer sewage.
embodiment 4
Certain chemical plant saliferous is containing ammonia containing wastewater from catalyst, and waste water quality is characterized as ammonia nitrogen concentration 50 ~ 100 mg/L, and COD concentration is 50 ~ 200mg/L, pH8.97, specific conductivity 15 ~ 25ms/cm, Cu
2+concentration is 5mg/L, Zn
2+concentration is 10mg/L.Adopt this waste water of SBR art breading, the impact due to metal ions such as high salt concentration and copper zinc, causes moving year sewage and can not realize processing up to standard all the time.Adopting the inventive method to process, is that 300mg/L adds according to MLSS first, and two days later, DO is 2 ~ 4mg/L in operation, and pH value is 7.8, and intrasystem nitrite nitrogen accounts for 50% of total nitration product, now adds the denitrification microbial inoculum C of embodiment 1.By the volume ratio that accounts for the amount of disposing of sewage 0.8%5 per hour, add first, every 5 days, add once, each dosage successively decreases 50% than last time.Move that after 20 days, water outlet ammonia nitrogen concentration is lower than 15mg/L, nitrate is lower than 15mg/L, and specific conductivity is lower than 1500 μ s/cm, Cu
2+concentration is lower than 0.5mg/L, Zn
2+concentration, lower than 1.0mg/L, has realized the up to standard processing of saliferous containing ammonia sewage.
Claims (16)
1. a high biological denitrification method containing salt catalyst sewage, unloading phase operating process temperature be 18-40 ℃, dissolved oxygen is 0.1 ~ 4 mg/L, pH is 7.0-9.0; Using sludge sewage as microbe carrier, at activated sludge concentration, add nitrobacteria under lower than 4000mg/L condition, what in system, nitrite nitrogen accounted for total nitration product more than 50% adds denitrification microbial inoculum, when ammonia nitrogen and nitrate are all lower than 50 mg/L, unloading phase, finishes, and enters steady running operation; The denitrification microbial inoculum using contain Staphylococcus cohnis (
staphylococcus cohnii) FSDN-C, and Arthrobacter (
arthrobacter creatinolyticus) FDN-1 and Shui Shi Flavobacterium (
flavobacterium mizutaii) at least one in FDN-2, three kinds of bacterial strains be preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", wherein Staphylococcus cohnis (
staphylococcus cohnii) the FSDN-C preservation time is on July 14th, 2011, deposit number is CGMCC NO.5062; Arthrobacter (
arthrobacter creatinolyticus)the FDN-1 preservation time, deposit number was CGMCC NO.3657 in order to be on March 11st, 2010; Shui Shi Flavobacterium (
flavobacterium mizutaii) the FDN-2 preservation time is on March 11st, 2010, deposit number is CGMCC NO.3659.
2. method according to claim 1, is characterized in that: sludge sewage is from oil refining sewerage treatment plant or catalyzer sewage work or municipal wastewater treatment plant.
3. method according to claim 1, is characterized in that: ammonia nitrogen concentration 30 ~ 600 mg/L of sewage, COD concentration is 30 ~ 1000mg/L, specific conductivity 5 ~ 60ms/cm, the Cu that contains 2 ~ 50mg/L
2+, 2 ~ 100mg/L Zn
2+, suspended substance is 10 ~ 1000mg/L, pH6 ~ 10.
4. method according to claim 1, is characterized in that: unloading phase operating process dissolved oxygen be 0.2 ~ 3mg/L, pH is 7.5-8.5.
5. method according to claim 1, is characterized in that: at activated sludge concentration, be to add nitrobacteria under 1000～3000mg/L condition.
6. method according to claim 1, is characterized in that: in system nitrite nitrogen account for total nitration product 50%～80% time add denitrification microbial inoculum.
7. method according to claim 1, is characterized in that: the nitrobacteria adding comprises Nitromonas and Nitrosomas, and Nitrosomas accounts for more than 60% nitrobacteria of nitrifier total quantity, and the nitrite nitrogen of reaction system accounts for the more than 50% of total nitration product.
8. method according to claim 1, is characterized in that: nitrobacteria dosage is that 100 ~ 1000mg/L adds according to MLSS.
9. method according to claim 1, is characterized in that: nitrobacteria is disposable to be added, or adds in batches, when in waste water, ammonia nitrogen concentration is lower than 50mg/L, stops adding; Denitrification microbial inoculum adds in batches, every 1～7 day, adds once.
10. according to the method described in claim 1 or 9, it is characterized in that: denitrification microbial inoculum adds by the volume ratio that accounts for the amount of disposing of sewage 0.01% ~ 1% per hour first, successively successively decrease later, each dosage successively decreases 30%～70% than last time, when nitrate stops adding during lower than 25mg/L.
11. methods according to claim 1, is characterized in that: in denitrification microbial inoculum, Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 are that thalline volume ratio is the mixing thalline of 1:0.1 ~ 3.
12. methods according to claim 1, is characterized in that: " Staphylococcus cohnis FSDN-C " is 1:0.1 ~ 10 with the ratio of " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " two bacteroids.
13. methods according to claim 1, is characterized in that: in denitrification microbial inoculum, contain nutritive substance and preservation auxiliary agent, the volume sum of thalline accounts for 10%～60% of denitrification denitrogenation microbial inoculum cumulative volume.
14. methods according to claim 9, is characterized in that: denitrification microbial inoculum divides and adds for 3 ~ 10 times.
15. methods according to claim 12, is characterized in that: " Staphylococcus cohnis FSDN-C " is 1:0.2 ~ 5 with the ratio of " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " two bacteroids.
16. methods according to claim 13, is characterized in that: in denitrification microbial inoculum, the volume sum of thalline accounts for 20%～50% of denitrification denitrogenation microbial inoculum cumulative volume.
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| CN104560859B (en) * | 2015-01-26 | 2018-03-16 | 鹭滨环保科技（上海）股份有限公司 | A kind of Staphylococcus pasteuri and preparation method thereof and the application in sewage is handled |
| CN106745793B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Advanced treatment method of salt-containing sewage |
| CN110699285B (en) * | 2019-10-24 | 2020-07-14 | 南京大学 | Palyalisma and application thereof in treating landfill leachate membrane concentrated solution |
| CN111285473B (en) * | 2019-12-28 | 2022-03-08 | 北京翰祺环境技术有限公司 | Method for promoting quick start of denitrification and decarburization system by using high-salt-resistant strain |
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| CN102153199A (en) * | 2011-01-26 | 2011-08-17 | 北京工业大学 | Device and method for short-range denitrification biological treatment of high-salt wastewater |
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| CN101565239A (en) * | 2009-05-23 | 2009-10-28 | 无锡绿水之源生物科技有限公司 | Method for preparing composite heterotrophic nitrobacteria water quality modifying solid powder or granule |
| CN102153199A (en) * | 2011-01-26 | 2011-08-17 | 北京工业大学 | Device and method for short-range denitrification biological treatment of high-salt wastewater |
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