CN103373762B - Biological denitrification method for salt-containing sewage - Google Patents
Biological denitrification method for salt-containing sewage Download PDFInfo
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Abstract
The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg/L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.
Description
Technical field
The invention belongs to technical field of sewage, relate in particular to a kind of bioremediation of saline sewage.In particular to a kind of peculiar microorganism microbial inoculum that utilizes to complete removing of ammonia nitrogen, total nitrogen and CODcr, realize the industrialization, the commercialization that are used for brine waste process microbiobacterial agent.
Background technology
In recent years, along with the development of industrial or agricultural, more and more stricter to the emission limit set of trade effluent, diffusion water of garbage burying ground and municipal effluent, sewage disposal technology more and more comes into one's own, and particularly the qualified discharge of ammonia-containing water becomes a process difficult problem for field of Environment Protection.Although the ammonia nitrogen in waste water can adopt the physico-chemical processes such as stripping stripping, ion-exchange, chemical oxidation to process, there is by product secondary pollution and the problem such as processing efficiency is low in these methods.By contrast, biological process is the better method controlling water body ammonia and nitrogen pollution.
Traditional biological method as the terminal technology of conventional sewage process, process containing ammonia sewage time usually to sacrifice the qualified discharge that load realizes ammonia nitrogen pollutant in waste water.The inorganic salts that in sewage, content is lower plays the vital role promoting enzyme reaction, maintain membrane equilibrium and adjustment osmotic pressure in microorganism growth process.But can microbial growth be suppressed when salt concn is too high and reduce microbic activity, bringing certain difficulty to biological treatment.Although all carried out repeatedly a large amount of improvement from the aspect such as technique and sewage treatment structure, certain effect is played in sewage treatment process, but because the main body active sludge of responsible ammonia nitrogen removal does not change, so the removal effect of ammonia nitrogen is not still very desirable.Ammonia nitrogen excessive problem directly has influence on the overall up to standard of Catalyst Production enterprise wastewater and normal production, becomes the bottleneck of restriction enterprise development, Ammonia Wastewater Treatment is become to the primary environmental issue of Catalyst Production enterprise.Therefore research and develop economic, practical, safe saline sewage bio-denitrification technology, to protection of the environment, promote the well-being of mankind significant.
No matter be traditional microorganic adhesion type wastewater treatment structures or high-performance bio film processing system newly developed, if be responsible for the microbial host Autotrophic nitrification bacterium of denitrogenation.The rate of propagation of autotrophic bacteria self is slow, cannot compete with heterotrophic organism in the Sludge System of mixed culture, be difficult to obtain higher biomass, nitrification efficiency is low, causes that autotrophic microorganism denitrification system impact resistance is weak, nitrification is incomplete, nitrogen removal rate is low.So some denitrification microorganisms that are novel, better effects if, as allotrophic nitrobacteria, aerobic denitrifying bacteria etc. are found in succession.
Allotrophic nitrobacteria fast growth, cell yield be high, require that dissolved oxygen concentration is low, also strong to the adaptive faculty of environment, compared with autotrophic type nitrifier, although the speed ratio autotrophic bacteria of the heterotrophic bacterium oxidation ammonium salt of biomass is slow, the speed of its overall oxidation ammonium salt is slow unlike autotrophic bacteria.Some heterotrophic microorganism can under the condition lacking organic carbon source, the oxidation carrying out ammonia obtains the energy needed for growing, and also can carry out ammonia oxidation under organism existent condition, not obtain energy, be a kind of metabolic process, the oxidation of ammonia is not by organic restriction.Therefore allotrophic nitrobacteria receives much attention as a kind of novel denitrification microorganism.Domestic and international investigator conducts extensive research in Heterotrophic nitrifier screening, functional metabolism approach, enzyme and gene etc., but still only rest on the laboratory study stage at present, really nitrification bacteria is applied in Practical Project the example processing waste water actually rare.
CN101302485A discloses a kind of heterotrophic nitrification microbial preparation, its cultural method and purposes, this microbial inoculum contain germ oligotrophy unit cell (
stenotrophomonas maltophiliastraindN 1.1) and pseudomonas putida (
pseudomonas putida straindN 1.2), this microbial inoculum can ammonia nitrogen in effective elimination water body and total nitrogen, can also remove the COD in organic waste water simultaneously, be applicable to high-concentration culture waste water process.This microbial inoculum is when process ammonia nitrogen concentration is the piggery wastewater of 455 ~ 600mg/L, and experiment moves to 94 ~ 95h, reaches 87% ~ 88% to the clearance of ammonia nitrogen in waste water, and process water outlet ammonia-nitrogen content is 59 ~ 72mg/L; Can by the total nitrogen process of water inlet 790mg/L to 164mg/L after process 95h, nitrogen removal rate is 79.2%.CN200910021020.7 discloses a kind of preparation method falling the water quality modifying microecological preparation of ammonia nitrogen and cultured water, and the probiotics of this invention belongs to aquaculture technology and ecological environmental protection technical field.The result of use of mentioned microorganism microbial inoculum in process saliferous ammonia-containing water is limited, needs to contain the suitable microbial inoculum of ammonia sewage development for saliferous and improve water treatment method.
Summary of the invention
Biological reinforcing technology provides new approaches to sewage treatment area, but existing biotechnological formulation is all not suitable for the denitrogenation processing of saline sewage.The invention discloses a kind of biological denitrification method of saline sewage, employing is added the close denitrification microorganism of growth conditions, by synchronous nitration and denitrification technique, is solved the qualified discharge problem of ammonia nitrogen pollutant in saline sewage.
The biological denitrification method of saline sewage of the present invention comprises following content: in biochemical wastewater treatment system, add denitrogenation microbial inoculum, start Simultaneous Nitrification and denitrification biological denitrogenation treating processes, the temperature of sewage disposal is 18-40 DEG C, optimum temperuture is 25-35 DEG C, dissolved oxygen is 0.1 ~ 5 mg/L, being preferably 0.2 ~ 2mg/L, pH is 7.0-9.0, is preferably 7.5-8.5.In denitrogenation microbial inoculum containing Staphylococcus cohnis (
staphylococcus cohnii) FSDN-C,
arthrobacter creatinolyticusfDN-1 and
flavobacterium mizutaiione or both in FDN-2, simultaneously containing Paracoccus denitrificans (
paracoccus denitrificans) DN-3 and
methylobacterium phyllosphaeraeone or both in SDN-3, respectively at being preserved on July 14th, 2011 and on March 11st, 2010, " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City (postcode 100101) to five kinds of bacterial strains.FSDN-C deposit number is CGMCC NO.5062, and the preservation time is on July 14th, 2011; FDN-1 deposit number is CGMCC No.3657, and the preservation time is on March 11st, 2010; FDN-2 deposit number is CGMCC No.3659, and the preservation time is on March 11st, 2010; DN-3 deposit number is CGMCC No.3658, and the preservation time is on March 11st, 2010; SDN-3 deposit number is CGMCC No.3660, and the preservation time is on March 11st, 2010.Containing the conventional additives such as nutritive medium, preservation auxiliary agent in denitrogenation microbial inoculum, in denitrogenation microbial inoculum, the volume sum of above-mentioned thalline accounts for 10% ~ 60% of denitrogenation microbial inoculum cumulative volume, is preferably 20% ~ 50%.
In the inventive method, saline sewage water quality characteristic is: ammonia nitrogen concentration 30 ~ 600 mg/L, and COD(Cr method is as follows) concentration be 300 ~ 3000mg/L, BOD concentration is 200 ~ 2000 mg/L, saliferous massfraction is 0.5% ~ 5%, pH is 6 ~ 10.
In the inventive method, denitrogenation microbial inoculum contains allotrophic nitrobacteria and denitrifying bacterium, growth and breeding speed is fast, and in a short time can not spoil disposal after adding, therefore, denitrogenation microbial inoculum needs to add when in system, activated sludge concentration is between 2000 ~ 5000mg/L, if activated sludge concentration is greater than after 5000mg/L needs first spoil disposal and adds microbiobacterial agent again in biochemical wastewater treatment system.
In the inventive method, denitrogenation microbial inoculum need add in batches, added once every 2 ~ 5 days, until water outlet ammonia nitrogen concentration lower than 50 mg/L preferably lower than 15mg/L, total nitrogen concentration preferably also can steady running can stop adding lower than 25mg/L lower than 50 mg/L for more than one week, terminate the unloading phase of Simultaneous Nitrification and denitrification, enter the steady running operational phase.
In the inventive method, dosage adds denitrogenation microbial inoculum according to 0.1% ~ 10% of volume of disposing of sewage per hour first, successively successively decreases later, and the biomass at every turn added than the last time successively decreases 30% ~ 50%.Adding rear Sewage treatment systems can not spoil disposal in three months.For batch process reactor, volume of disposing of sewage per hour is average volume of disposing of sewage per hour in each treatment cycle.
In the microbiobacterial agent related in the inventive method, Staphylococcus cohnis FSDN-C colony colour is white, and bacterial strain individuality is in spherical, and without gemma, gramstaining is positive, and catalase is positive, and oxidase negative, can utilize several kinds of carbon source.Arthrobacter FDN-1 colony colour is yellow, bacterial strain is individual is bar-shaped, without gemma, can move; Gramstaining is positive, and catalase is positive, and oxidase negative, can utilize several kinds of carbon source.Shui Shi Flavobacterium FDN-2 colony colour is white, bacterial strain is individual is shaft-like, without gemma; Gramstaining is negative, and catalase is positive, and oxidase positive, can utilize several kinds of carbon source.Paracoccus denitrificans DN-3 bacterium colony is milk yellow; The individual ovalize of bacterial strain; Gramstaining is negative, and catalase is positive, oxidase positive; Cheap carbon source can be utilized.Methylobacterium SDN-3 bacterium colony is orange, is Gram-negative bacteria, and thalline is shaft-like, can move; Circular tiny, neat in edge is smooth; Catalase is positive, and oxidase positive, can utilize several kinds of carbon source.
Staphylococcus cohnis FSDN-C in microbial inoculum of the present invention, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2, Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 all can complete denitrification process under aerobic and anaerobic environment.Wherein Staphylococcus cohnis FSDN-C can be that nitrogenous source carries out denitrification denitrogenation with nitrite nitrogen, can remove COD while denitrogenation; Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 can be that nitrogenous source carries out denitrification denitrogenation with nitrite nitrogen, can remove COD while denitrogenation; Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 all can carry out heterotrophic nitrification-aerobic denitrification simultaneous denitrification using ammonia nitrogen as nitrogenous source, can remove COD while denitrogenation.
In microbiobacterial agent of the present invention, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2 can mix in any proportion but must comprise wherein a kind of, and Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 also can carry out mixing according to arbitrary proportion but must comprise wherein a kind of." Staphylococcus cohnis FSDN-C, " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " are 1:0.1 ~ 10:0.1 ~ 10 with the ratio of " Paracoccus denitrificans DN-3 and/or Methylobacterium SDN-3 " three bacteroids, are preferably 1:0.2 ~ 5:0.2 ~ 5.(by thalline volumeter, thalline volume be cultivate after under per minute 10,000 turns of conditions the thalline volume that obtain of centrifugation after 5 minutes, lower with).In microbiobacterial agent of the present invention, can containing suitable additive, as nutritive substance, preservation auxiliary agent etc., concrete additive types and consumption are well known to those skilled in the art.
The concrete preparation method of one of wastewater treatment microbial microbial inoculum of the present invention comprises following content:
1, Staphylococcus cohnis FSDN-C of the present invention, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2, the secondary coccus DN-3 and Methylobacterium SDN-3 of nitrogen are inoculated in respectively on solid medium and activate;
2, being inoculated in corresponding liquid medium respectively with connecing the bacterium colony that collarium makes even on plate, under temperature 25 ~ 35 DEG C, 150 ~ 240rpm aerobic condition, shaking cultivation 1 ~ 3 day to logarithmic phase, obtaining liquid bacterial agent seed liquor;
3, collect thalline by after above-mentioned seed liquor amplification culture, in the mixing of required ratio after filtering and concentrating, add the additive that required nutritive medium etc. is required, be microbiobacterial agent of the present invention.The seed liquor of often kind of bacterium can amplification culture separately, also FDN-1 seed liquor and FDN-2 seed liquor common amplification culture can be mixed in proportion, DN-3 seed liquor and SDN-3 seed liquor are mixed in proportion common amplification culture, also can be that amplification culture is carried out in the seed liquor mixing of four kinds of bacterium.
The thalline activation of the Staphylococcus cohnis FSDN-C involved by microbiobacterial agent of the present invention and seed liquor are cultivated culture medium prescription used and are: extractum carnis: 5 ~ 10g/L, peptone: 8 ~ 15g/L, NaNO
2: 0.4 ~ 1.0g/L, methyl alcohol: 0.25 ~ 1.0mL/L.The thalline activation of the Arthrobacter FDN-1 involved by microbiobacterial agent of the present invention and Shui Shi Flavobacterium FDN-2 and seed liquor are cultivated culture medium prescription used and are: extractum carnis: 3 ~ 7g/L, peptone: 7 ~ 13g/L, NaNO
2: 0.8 ~ 1.5g/L, solid medium adds the agar of 1.5 ~ 2.5%.The thalline activation of the Paracoccus denitrificans DN-3 involved by microbiobacterial agent of the present invention and Methylobacterium SDN-3 and seed liquor are cultivated culture medium prescription used and are: ammonium sulfate: 0.1 ~ 0.5g/L, KNO
3: 0.5 ~ 1.0g/L, Soduxin 2 ~ 8g/L, methyl alcohol: 0.25 ~ 1.0mL/L, solid medium adds the agar of 2%; In addition also a small amount of iron ion and magnesium ion etc. is contained in nutrient solution.Culture condition is: temperature is 20 ~ 35 DEG C, 150 ~ 240rpm concussion is cultured to logarithmic phase and can gathers in the crops thalline for the preparation of microbiobacterial agent.
Microbiobacterial agent amplification culture of the present invention nutrient solution used can be autogamy or actual waste water, and the total nitrogen in nutrient solution and ammonia nitrogen concentration are 100mg/L ~ 1000mg/L, carbon-nitrogen mass ratio 5:1 ~ 15:1; Culture condition is temperature 15 DEG C ~ 35 DEG C; PH6.5 ~ 10.0; Dissolved oxygen is lower than 3.0mg/L.The present invention's amplification culture reactor used can be various suitable configurations, has good aeration and stirring system.In culturing process, pH value does not need to regulate.
The bacteria suspension that concentration is higher is obtained after the concentration of above-mentioned amplification culture; liquid microbial inoculum can be prepared into after these bacteria suspensions being added nutritive medium or nutritive medium and protectant mixed solution, the existing method in this area also can be utilized to be prepared into the microbial inoculum of dry powder.The for shelf-stable such as plastics bag, Plastic Bottle or plastic tank, antifreeze, the container of being convenient to the materials such as transport or equipment can be adopted to pack.Packaging Method adopts the existing routine techniques in this area, and Packing Unit is that the microbial inoculum after 50 ~ 5000g/ bag (bag or bottle) packaging can be preserved as the case may be under room temperature or cold condition.The room temperature preservation quality guaranteed period is 1 ~ 6 month, and the cryopreservation quality guaranteed period is 1 ~ 5 year, and after preserving, active reduced rate is less than 10%.
The present invention's concentration method used can not affect the method for microbial activity by centrifugal or filtration etc.
First microbial inoculum preparation process of the present invention carries out thalline activation; Then carry out seed liquor cultivation, finally will collect thalline after seed liquor amplification culture, concentrate, pack and save backup.The microbiobacterial agent tolerance obtained and strong adaptability, good impact resistance, the elimination capacity of total nitrogen is high, treatment effect good; Directly can be added in Waste Water Treatment or with active sludge and mix or biofilm aftertreatment waste water on various filler, use properties is good; Microbiobacterial agent resume speed through certain hour preservation is fast, and biological activity is high, can realize the industrialization of denitrification microorganism, commercialization.
The saline sewage biological denitrification method that the present invention proposes, mainly by directly to add by Staphylococcus cohnis FSDN-C, Arthrobacter (
arthrobacter creatinolyticus) FDN-1, Shui Shi Flavobacterium (
flavobacterium mizutaii) FDN-2, Paracoccus denitrificans (
paracoccus denitrificans) DN-3 and Methylobacterium (
methylobacterium phyllosphaerae) the denitrogenation microbial inoculum of the bacterial strain such as SDN-3 composition realizes.Described different microorganism works in coordination, competes substrate mutually, after particularly supplementing Staphylococcus cohnis FSDN-C, enhances the salt resistant character of Sewage treatment systems, improves adsorptivity and the flocculence of mud.Because the scope of application of population effect to waste water quality is wide, strong to the withstand shock ability of saline sewage, while pollutent such as removal ammonia nitrogen and COD etc., significantly improve the treatment effect of sewage.
Embodiment
The bioremediation of a kind of saline sewage that the present invention proposes, liquid microbial inoculum fast growth used, collecting amount is large, and microbial inoculum has stronger tolerance and adaptability, has good shock resistance; Directly can be added in waste water treatment plant's active sludge and use, also can process saliferous containing ammonia sewage in suitable biochemical reactor.
the preparation method of embodiment 1 microbiobacterial agent
1, thalline activation: the thalline activation of Staphylococcus cohnis FSDN-C and seed liquor are cultivated culture medium prescription used and be: extractum carnis: 6g/L, peptone: 8g/L, NaNO
2: 0.6g/L, methyl alcohol: 0.5mL/L; The activation culture based formulas of Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 is: extractum carnis: 5g/L, peptone: 10g/L, NaNO
2: 1g/L, adds the agar of 2.0%; The activation culture based formulas of Paracoccus denitrificans DN-3 is: KNO
3: 1g/L, Soduxin: 8g/L, KH
2pO
4: 1g/L, FeCL
2: 0.5g/L, adds the agar of 2.0%; The activation culture based formulas of Methylobacterium SDN-3 is: ammonium sulfate: 0.5g/L, methyl alcohol: 0.75mL/L, KH
2pO
4: 1g/L, FeCL
2: 0.5g/L; Add 2% agar.It is activate in 30 DEG C of constant incubators that flat board is placed on temperature after coating evenly.
2, be inoculated in corresponding liquid medium respectively with the thalline connect on collarium scraping flat board, under temperature 25 ~ 35 DEG C, 150 ~ 240rpm aerobic condition, shake cultivation 1 ~ 3 day to logarithmic phase, obtain liquid bacterial agent seed liquor; Culture medium prescription, with activation culture based formulas, need not add agar.
3, using above-mentioned Staphylococcus cohnis FSDN-C as No. I seed liquor, above-mentioned Arthrobacter FDN-1 and Shui Shi Flavobacterium FDN-2 is mixed as No. II seed liquor (thalline is according to 1:1 and 0.5:1 two kinds of ratio combination numbering II-1 and II-2 respectively), above-mentioned Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 is mixed as No. III seed liquor (thalline is according to 1:1 and 0.5:1 two kinds of ratio combination numbering III-1 and III-2 respectively), No. I seed liquor, arbitrary group in No. II seed liquor or any one microorganism mix according to 1:1:1 and 1:2:3 two kinds of ratios with arbitrary group in No. III seed liquor or any one microorganism again and in the reactor with good stirring system, carry out amplification culture respectively, ammonia nitrogen concentration in nutrient solution is 200mg/L ~ 800mg/L, carbon-nitrogen mass ratio 5:1 ~ 10:1, culture condition is temperature 25 DEG C ~ 35 DEG C, pH6.5 ~ 10.0, dissolved oxygen is lower than 3.0mg/L.
To the liquid bacterial suspension A obtained through amplification culture (seed liquor I, seed liquor II-1 and seed liquor III-1 blending ratio 1:1:1), B(seed liquor I, seed liquor II-2 and seed liquor III-2 blending ratio 1:2:3), C(seed liquor I, seed liquor II-1 and seed liquor III-2 blending ratio 1:1:1), D(seed liquor I, Arthrobacter FDN-1 and seed liquor III-2 blending ratio 1:2:3), E(seed liquor I, seed liquor II-2 and Paracoccus denitrificans DN-3 blending ratio 1:1:3) collect, concentrated, then add the nutritive medium of bacteria suspension two volumes.NH in often liter of nutritive medium
4 +-N, Fe
2+, Mg
2+, K
+, Ca
2+these five kinds of cationic mole of allocation ratios are 2000:5:20:20:15, then are dispensed in the Plastic Bottle of 500ml after the preserving agent adding bacteria suspension volume 2.5%, save backup under putting-70 DEG C of conditions.
embodiment 2
Certain chemical plant saline sewage water quality characteristic is: ammonia nitrogen 60 ~ 100 mg/L, and COD(Cr method is as follows) 300 ~ 1000mg/L, BOD concentration is 200 ~ 700, saliferous massfraction is 2 ~ 5%, pH8.5.
In system During Process of Long-term Operation, temperature is about 25 DEG C, dissolved oxygen be 0.1 ~ 4.5mg/L, pH is 7.8 ~ 8.2.Impact due to salt causes ammonia nitrogen removal weak effect in system, and after process, total nitrogen concentration is up to 150mg/L, and the SVI of active sludge is greater than 200 mL/g.Adopt the inventive method to add microbial inoculum in Sewage treatment systems, add microbial inoculum A first, add once later every three days according to 1.5% of handled sewage quantity in Sewage treatment systems, the biomass at every turn added than the last time successively decreases 30%.Complete and add rear system cloud gray model one week for 5 times, analyzing and testing water outlet ammonia nitrogen and nitrate all stop adding lower than two kinds of thalline.When not having spoil disposal in three months, water outlet total nitrogen concentration is greater than 85% lower than 25mg/L, COD clearance all the time, and the SVI of active sludge is 150 mL/g.As can be seen here, adopt this programme to achieve the biological denitrificaion process of saline sewage, improve the settling property of mud simultaneously.
embodiment 3
Certain chemical plant saline sewage water quality characteristic is: ammonia nitrogen 100 ~ 300 mg/L, and COD(Cr method is as follows) 500 ~ 600mg/L, BOD concentration is 200 ~ 300, saliferous massfraction is 1 ~ 2%, pH7.8.
In system During Process of Long-term Operation, temperature is about 28 DEG C, dissolved oxygen be 1.0 ~ 5.5mg/L, pH is 8.0 ~ 8.2.In system During Process of Long-term Operation, ammonia nitrogen removal frank only has 50%.Adopt the inventive method, add microbial inoculum C first, add once later every five days according to 5% of handled sewage quantity in Sewage treatment systems, the biomass at every turn added than the last time successively decreases 40%.Complete and add rear system cloud gray model one week for 6 times, analyzing and testing water outlet ammonia nitrogen removal frank reaches 85%, and two kinds of thalline all stop adding.When not having spoil disposal in three months, water outlet ammonia nitrogen and nitrogen removal rate are stabilized in more than 85% always, achieve the biological denitrificaion process of saline sewage.
embodiment 4
Certain chemical plant saline sewage water quality characteristic is: ammonia nitrogen concentration 400 ~ 500 mg/L, and COD(Cr method is as follows) concentration be about 2000mg/L, BOD concentration is about 1500, saliferous massfraction is 0.5 ~ 2%, pH8.7.In system During Process of Long-term Operation, temperature is about 24 DEG C, dissolved oxygen be 2.0 ~ 6.5mg/L, pH is 8.2 ~ 8.5.In system During Process of Long-term Operation, ammonia nitrogen removal frank only has 60%.Adopt the inventive method, add microbial inoculum E first, add once later every 3 days according to 8% of handled sewage quantity in Sewage treatment systems, the biomass at every turn added than the last time successively decreases 50%.Complete and add rear system cloud gray model one week for 8 times, analyzing and testing water outlet ammonia nitrogen removal frank reaches 90%, and two kinds of thalline all stop adding.When not having spoil disposal in three months, water outlet ammonia nitrogen and nitrogen removal rate are stabilized in more than 90% always, achieve the biological denitrificaion process of saline sewage.
Claims (11)
1. the biological denitrification method of a saline sewage, it is characterized in that comprising following content: in biochemical wastewater treatment system, add denitrogenation microbial inoculum, start Simultaneous Nitrification and denitrification biological denitrogenation treating processes, the temperature of sewage disposal is 18-40 DEG C, dissolved oxygen is 0.1 ~ 5 mg/L, pH is 7.0-9.0; In described denitrogenation microbial inoculum containing Staphylococcus cohnis (
staphylococcus cohnii) FSDN-C, Arthrobacter (
arthrobacter creatinolyticus) FDN-1 and Shui Shi Flavobacterium (
flavobacterium mizutaii) one or both in FDN-2, simultaneously containing Paracoccus denitrificans (
paracoccus denitrificans) DN-3 and Methylobacterium (
methylobacterium phyllosphaerae) one or both in SDN-3, five kinds of bacterial strains respectively at being preserved on July 14th, 2011 and on March 11st, 2010 " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", deposit number is respectively CGMCC NO.5062, CGMCC No.3657, CGMCC No.3659, CGMCC No.3658, CGMCC No.3660; FSDN-C deposit number is CGMCC NO.5062, and the preservation time is on July 14th, 2011; FDN-1 deposit number is CGMCC No.3657, and the preservation time is on March 11st, 2010; FDN-2 deposit number is CGMCC No.3659, and the preservation time is on March 11st, 2010; DN-3 deposit number is CGMCC No.3658, and the preservation time is on March 11st, 2010; SDN-3 deposit number is CGMCC No.3660, and the preservation time is on March 11st, 2010.
2. method according to claim 1, is characterized in that: the temperature of sewage disposal is 25-35 DEG C, and dissolved oxygen is 0.2 ~ 2mg/L, pH is 7.5-8.5.
3. method according to claim 1, is characterized in that: containing nutritive medium and preservation auxiliary agent in denitrogenation microbial inoculum, in denitrogenation microbial inoculum, the volume sum of thalline accounts for 10% ~ 60% of denitrogenation microbial inoculum cumulative volume.
4. method according to claim 1, is characterized in that: saline sewage water quality characteristic is, ammonia nitrogen concentration 30 ~ 600 mg/L, and COD concentration is 300 ~ 3000mg/L, BOD concentration is 200 ~ 2000 mg/L, and saliferous massfraction is 0.5% ~ 5%, pH is 6 ~ 10.
5. method according to claim 1, is characterized in that: denitrogenation microbial inoculum adds when in biochemical treatment system, activated sludge concentration is between 2000 ~ 5000mg/L.
6. method according to claim 1 or 5, it is characterized in that: denitrogenation microbial inoculum need add in batches, added once every 2 ~ 5 days, until water outlet ammonia nitrogen concentration is lower than 50 mg/L, total nitrogen concentration also can add lower than 50 mg/L in steady running more than one week stopping, terminate the unloading phase of Simultaneous Nitrification and denitrification, enter the steady running operational phase.
7. method according to claim 6, it is characterized in that: water outlet ammonia nitrogen concentration is lower than 15mg/L, total nitrogen concentration is lower than 25mg/L and energy steady running more than one week stopping add denitrogenation microbial inoculum, terminates, enter the steady running operational phase unloading phase of Simultaneous Nitrification and denitrification.
8. method according to claim 6, is characterized in that: the dosage first of denitrogenation microbial inoculum adds according to 0.1% ~ 10% of volume of disposing of sewage per hour, successively successively decreases later, and the biomass at every turn added than the last time successively decreases 30% ~ 50%.
9. the method according to claim 1 or 3, it is characterized in that: in denitrogenation microbial inoculum, Arthrobacter FDN-1, Shui Shi Flavobacterium FDN-2 mixes in any proportion but must comprise wherein a kind of, and Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 carries out mixing according to arbitrary proportion but must comprise wherein a kind of; " Staphylococcus cohnis FSDN-C, " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " are 1:0.1 ~ 10:0.1 ~ 10 with the volume ratio of " Paracoccus denitrificans DN-3 and/or Methylobacterium SDN-3 " three bacteroids.
10. method according to claim 9, is characterized in that: " Staphylococcus cohnis FSDN-C, " Arthrobacter FDN-1 and/or Shui Shi Flavobacterium FDN-2 " are 1:0.2 ~ 5:0.2 ~ 5 with the volume ratio of " Paracoccus denitrificans DN-3 and/or Methylobacterium SDN-3 " three bacteroids.
11. methods according to claim 3, is characterized in that: containing nutritive medium and preservation auxiliary agent in denitrogenation microbial inoculum, in denitrogenation microbial inoculum, the volume sum of thalline accounts for 20% ~ 50% of denitrogenation microbial inoculum cumulative volume.
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| CN103374525B (en) * | 2012-04-29 | 2014-08-20 | 中国石油化工股份有限公司 | Wastewater treating microbial agent and preparation method thereof |
| CN103374524B (en) * | 2012-04-29 | 2014-08-20 | 中国石油化工股份有限公司 | Salt-tolerant microbial agent and preparation method thereof |
| CN105621610B (en) * | 2014-10-28 | 2018-01-16 | 中国石油化工股份有限公司 | A kind of saline sewage simultaneous nitrification-denitrification denitrogenation method |
| CN105621611B (en) * | 2014-10-28 | 2018-02-09 | 中国石油化工股份有限公司 | A kind of quick start method of ammonia-containing water short-cut nitrification and denitrification |
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| CN104560812B (en) * | 2014-12-30 | 2017-03-15 | 南京农业大学 | The secondary coccus of one facultative anaerobic arsenic oxidation and its application |
| CN106630172B (en) * | 2015-11-04 | 2019-09-10 | 中国石油化工股份有限公司 | A kind of processing method of epoxychloropropane waste water |
| CN106635859B (en) * | 2015-11-04 | 2020-03-17 | 中国石油化工股份有限公司 | Open culture method and application of paracoccus FSTB-2 |
| CN106635860B (en) * | 2015-11-04 | 2019-07-12 | 中国石油化工股份有限公司 | A kind of Salt-tolerant microbial agent and its preparation method and application |
| CN106635858B (en) * | 2015-11-04 | 2020-03-17 | 中国石油化工股份有限公司 | Paracoccus and culture application thereof |
| CN106746159B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Treatment method of antibiotic production wastewater |
| CN106745793B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Advanced treatment method of salt-containing sewage |
| CN106754450B (en) * | 2015-11-19 | 2020-12-08 | 中国石油化工股份有限公司 | Denitrifying microorganism culture promoter and preparation method and application thereof |
| CN106745727B (en) * | 2015-11-19 | 2020-10-16 | 中国石油化工股份有限公司 | Denitrification method for ammonia-containing wastewater discharged in acrylic fiber production process |
| CN108117225A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | A kind of processing method of high slat-containing wastewater |
| CN108117221B (en) * | 2016-11-29 | 2021-01-05 | 中国石油化工股份有限公司 | Treatment method of reverse osmosis concentrated water |
| CN108117220A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | A kind of integrated conduct method of high slat-containing wastewater |
| CN110551655A (en) * | 2019-09-05 | 2019-12-10 | 北京星皓互动科技有限公司 | composite biological agent, preparation method thereof and application thereof in printing and dyeing textile sewage treatment |
| CN116814483B (en) * | 2023-06-15 | 2024-07-16 | 黄河三角洲京博化工研究院有限公司 | Efficient anoxic denitrification strain and application thereof |
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| JP2005177695A (en) * | 2003-12-22 | 2005-07-07 | Nishihara Neo Co Ltd | Wastewater treatment system |
| KR100722929B1 (en) * | 2006-07-28 | 2007-05-30 | 이인형 | Advanced treatment method of ethanolamine-wastewater based on coupled process with physicochemical and biological unit operations |
| CN101724583B (en) * | 2008-10-29 | 2012-05-30 | 中国石油化工股份有限公司 | Method for producing microorganisms for processing ammonia-containing wastewater |
| CN101899401B (en) * | 2009-05-25 | 2013-07-24 | 中国石油化工股份有限公司 | Microbial agent for treating ammonia-containing waste water and preparation method thereof |
| CN102040281B (en) * | 2009-10-21 | 2014-12-31 | 中国石油化工股份有限公司 | Method for culturing denitrogenized granular sludge |
| CN102311166B (en) * | 2010-07-07 | 2013-05-01 | 中国石油化工股份有限公司 | Method for realizing shortcut nitration of high ammonia nitrogen wastewater |
| CN102241459B (en) * | 2011-06-01 | 2012-10-10 | 北京大学 | Method for reinforcing adsorption-biodegradation (AB) denitrification technology by use of heterotrophic nitrification-aerobic denitrification bacteria |
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