CN103305443B - Preparation and method for restoring ammonia nitrogen-containing industrial sewage - Google Patents
Preparation and method for restoring ammonia nitrogen-containing industrial sewage Download PDFInfo
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- CN103305443B CN103305443B CN201310241368.2A CN201310241368A CN103305443B CN 103305443 B CN103305443 B CN 103305443B CN 201310241368 A CN201310241368 A CN 201310241368A CN 103305443 B CN103305443 B CN 103305443B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 239000010865 sewage Substances 0.000 title claims abstract description 21
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title description 31
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 12
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 12
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims abstract description 12
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 12
- 241000186074 Arthrobacter globiformis Species 0.000 claims abstract description 11
- 241001509286 Thiobacillus denitrificans Species 0.000 claims abstract description 11
- 241000588813 Alcaligenes faecalis Species 0.000 claims abstract description 3
- 229940005347 alcaligenes faecalis Drugs 0.000 claims abstract description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 6
- 230000008439 repair process Effects 0.000 claims description 4
- 241001103617 Pseudomonas aeruginosa ATCC 15442 Species 0.000 claims description 3
- 241001135759 Sphingomonas sp. Species 0.000 claims description 2
- 238000011282 treatment Methods 0.000 abstract description 10
- 241000736131 Sphingomonas Species 0.000 abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 239000002351 wastewater Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 230000031018 biological processes and functions Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
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- 230000004151 fermentation Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 238000005273 aeration Methods 0.000 description 3
- 238000005660 chlorination reaction Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 239000008176 lyophilized powder Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000002068 microbial inoculum Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
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- 238000012258 culturing Methods 0.000 description 2
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- 230000003203 everyday effect Effects 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
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- 150000004676 glycans Chemical class 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
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- 239000000126 substance Substances 0.000 description 2
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- 239000002699 waste material Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
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- 238000000909 electrodialysis Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
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- 239000003337 fertilizer Substances 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
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- 238000003973 irrigation Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000001965 potato dextrose agar Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a preparation for restoring ammonia nitrogen-containing industrial sewage. The preparation is prepared from the following components: 100 parts of thiobacillus denitrificans, 80 parts of bacillus licheniformis, 60 parts of sphingomonas, 50 parts of phanerochaete chrysosporium, 40 parts of arthrobacter globiformis, 30 parts of alcaligenes faecalis, 10 parts of pseudomonas aeruginosa and 10 parts of bacillus cereus. The invention also discloses a preparation method of the preparation. The preparation disclosed by the invention is suitable for the treatment of high-ammonia nitrogen industrial sewage, is energy-saving and environment-friendly, and has better application prospects.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to preparation and method that a kind of reparation contains ammonia nitrogen industrial sewage.
Background technology
Industrial pollution water body refers to the waste water, sewage and the waste liquid that produce in Industrial processes, wherein containing the pollutent produced in the industrial production materials, intermediate product and the product that outflow with water and production process.Along with developing rapidly of industry, kind and the quantity of waste water rapidly increase, also increasingly extensive and serious to the pollution of water body, threaten the health and safety of the mankind.Therefore, for protection of the environment, the process of trade effluent is more even more important than the process of municipal effluent.
The composition of industrial sewage is more complicated, particularly a large amount of artificial-synthetic compound's entered environments, this kind of material mainly alkanes, ammonia nitrogen class, olefines, alicyclic hydrocarbon type, arene, phosphide etc., due to complicacy and the biological strange property of these these body structures of material, utilization can not be decomposed by the microorganisms at short notice, the microorganism of traditional method of wastewater treatment activated sludge culture can not be removed these pollutents effectively, these materials accumulate for a long time in the environment, the ecotope of depending on for existence to us causes very large pollution, very large harm is brought to the physical and mental health of the mankind.Quite a few industrial pollution enterprise of China would rather be punished also to be unwilling to invest and administer waste water, even if there have waste disposal plant to run to be also abnormal.Therefore, develop that a kind of construction investment is few, running cost is low, the sewage disposal technology of good treatment efficiency is extremely urgent.
According to the difference of ammonia nitrogen in sewage concentration, waste water can be divided into 3 classes: high-concentration ammonia nitrogenous wastewater (NH3-N > 500mg/l), intermediate concentration ammonia nitrogen waste water (NH3-N:50-500mg/l), Low Concentration Ammonia Containing Wastewater (NH3-N < 50mg/l).But the ammonia nitrogen waste water of high density has restraining effect to microbic activity, constrain biochemical process to its process application and effect, the degradation efficiency of biochemical system to organic pollutant can be reduced simultaneously, thus cause process water outlet to be difficult to reach requirement.The main method of removal ammonia nitrogen conventional in prior art has: Physical, chemical method, biological process.Physical is containing treatment technologies such as reverse osmosis, distillation, soil irrigations; Chemical method is containing treatment technologies such as ion-exchange, ammonia aeration, break point chlorination, burning, chemical precipitation, catalytic pyrolysis, electrodialysis, electrochemistry; Biological process is containing treatment technologies such as algal culture, biological nitration, immobilized biotechnologies.Method practical at present has: break point chlorination method, selective ion exchange method, ammonia aeration method, biological process and chemical precipitation method.Wherein, break point chlorination method, selective ion exchange method, ammonia aeration method are not suitable for large-scale industrialization application, and chemical process easily causes other to pollute, and cost is higher, and the method for the most economic environmental protection is at present biological process.
Summary of the invention
The object of this invention is to provide a kind of repair containing ammonia nitrogen industrial sewage preparation and utilize said preparation to dispose of sewage the method for ammonia nitrogen.
Sewage disposal composite fungus agent of the present invention, reasonable compatibility between each bacterial classification, symbiosis is coordinated, mutually not antagonism, and its preparation method is easy, easy to implement the method, and it is easy and simple to handle, is beneficial to suitability for industrialized production.
The present invention adopts following technical scheme to realize: a kind of preparation repaired containing ammonia nitrogen industrial sewage, and the activeconstituents of said preparation comprises the raw material bacterium of following weight part:
Thiobacillus denitrificans 100 parts, Bacillus licheniformis 80 parts, Sphingomonas 60 parts, Phanerochaete chrysosporium 50 parts, Arthrobacter globiformis 40 parts, Bacillus foecalis alkaligenes 30 parts, pseudomonas aeruginosa 10 parts, bacillus cereus 10 parts.
1) described thiobacillus denitrificans (Thiobacillus denitrificans) specifically can be preferred ATCC25259 (such as reference APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May2007, p.3265-3271)
2) described Bacillus licheniformis (Bacillus licheniformis) specifically can be preferably CCTCC NO.M206082 (CN101037659A)
3) described Sphingomonas (Sphingomonas sp.) is preferably CGMCC NO.4589 (CN102168054A);
4) described Phanerochaete chrysosporium (Phanerochaete chrysosporium) preferably ATCC24725 (such as APPLIEDAND ENVIRONMENTAL MICROBIOLOGY, Feb1994, p709-714);
5) described Arthrobacter globiformis (Arthrobacter globiformi s) is preferably CCTCC NO:M209015 (CN101492649A)
6) described Bacillus foecalis alkaligenes (Alcaligenes faecalis) is preferably Bacillus foecalis alkaligenes ATCC 31555 (for example, see document Structural studies of an extracellular polysaccharide (S-130) elaborated byAlcaligenesATCC31555, Carbohydrate Research, 1986);
7) described pseudomonas aeruginosa (Pseudomonas aeruginosa) specifically can be pseudomonas aeruginosa ATCC 15442 (for example, see document Adaptation of Pseudomonas aeruginosa ATCC 15442 todidecyldimethylammonium bromide induces changes in membrane fatty acid compositionand in resistance of cells, Journal of Applied Microbiology, 2001);
8) described bacillus cereus (Bacillus cereus) specifically can be ATCC10876 (such as New J.Chem., 2007,31,748-755).
Bacterial classification of the present invention all can be bought from CGMCC, CCTCC and American Type Culture collection warehousing (ATCC) and obtain.
The existence form of composite fungus agent of the present invention is preferably the mixture of lyophilized powder, microbial inoculum or microbial inoculum and solid adjuvant material.When the existence form of described microbial inoculum is lyophilized powder, preparation method comprises and above-mentioned bacterium is prepared fermented liquid respectively, after mass ratio mixing, makes lyophilized powder by this area ordinary method.
Wherein each strain fermentating liquid preparation process is preferably in the following manner:
The independent enlarged culturing of various bacterial classification:
The training method of thiobacillus denitrificans: be inoculated into (Na on substratum
2s
2o
35H
2o 5g, KNO
32g, KH
2pO
42g, NaHCO
31g, MgCl
26H2O0.5g, FeSO47H2O0.01g NH
4cl0.5g, water 1000ml), 28-30 DEG C, makes primary inclined plane and cultivates, and in then secondary seed cultivation, mixing fermentation culture to product, viable count reaches 1 × 10
8individual/gram.
The cultivation of Bacillus licheniformis or bacillus cereus: first withered Bacillus licheniformis or bacillus cereus test tube kind are seeded on beef-protein medium, 28-30 DEG C, make primary inclined plane to cultivate, then be inoculated in triangular flask and do the cultivation of vibration secondary liquid, then proceed to liquid fermentation tank and do three grades of liquid culture, finally be inoculated on solid medium and do level Four cultivation, to product, viable count reaches 1 × 10
8individual/gram.
The training method of pseudomonas aeruginosa or Bacillus foecalis alkaligenes: pseudomonas aeruginosa or Bacillus foecalis alkaligenes are first on substratum, and 28-30 DEG C, makes primary inclined plane and cultivate, and in then secondary seed cultivation, mixing fermentation culture to product, viable count reaches 1.0 × 10
8individual/gram, described medium component is: NH
4cl 1.0g, CH
3cOONa 3.5g, MgCl
20.1g, CaCl
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000ml, pH7.2.
The training method of Sphingomonas: first Sphingomonas is seeded on ox yeast extract paste protein culture medium substratum, 28-30 DEG C, then makes viable count in level liquid seed culture, secondary liquid seed culture and fermentor cultivation to product and reaches 1.0 × 10
8individual/gram.
The training method of Phanerochaete chrysosporium or Arthrobacter globiformis: first Phanerochaete chrysosporium is inoculated on potato dextrose agar, 28-30 DEG C, make primary inclined plane to cultivate, then be inoculated in triangular flask and do the cultivation of vibration secondary liquid, then proceed to liquid fermentation tank and do three grades of liquid culture, finally be inoculated on solid medium and do level Four cultivation, to product, viable count reaches 1 × 10
8individual/gram.
Be uniformly mixed by described weight part by the bacterium of above-mentioned cultivation, then carry out cryodrying, drying temperature is 20-30 DEG C, and after dry, water content is 20%; Finally carry out packing and get final product.
Note, in above-mentioned steps, strain expanded culture and the method for preparing solid fungicide are not unique, and those skilled in the art can select suitable substratum and enlarged culturing method according to general knowledge, make viable count reach 10
8individual/gram, and conveniently prepare the method preparation of solid fungicide.
Composite fungus agent of the present invention is by the various bacterial classification that can form dominant microflora, be mixed with high-efficiency microorganism preparation, be rich in the Waste Water Treatment of ammonia nitrogen by a certain amount of being added to, accelerate the degraded of microbe, to improve the biological treatment efficiency of system, ensure system stable operation.Its pollutent containing ammonia nitrogen has the microorganism of excellent degradation capability, reasonable compatibility between each bacterial classification, and symbiosis is coordinated, mutually not antagonism, and active high, biomass is large, and breeding is fast.Composite fungus agent of the present invention is suitable for Industrial Waste Water Treatments, can improve the process water yield and water quality treatment, reduces working cost, promotes qualified discharge.
Embodiment
By adopting the mode of specific embodiment, the present invention is further elaborated below, but it should not be construed the restriction to core initiative spirit of the present invention.
Embodiment 1
Repair the preparation containing ammonia nitrogen industrial sewage, said preparation is prepared from by the raw material bacterium of following weight part:
Thiobacillus denitrificans 100 parts, Bacillus licheniformis 80 parts, Sphingomonas 60 parts, Phanerochaete chrysosporium 50 parts, Arthrobacter globiformis 40 parts, Bacillus foecalis alkaligenes 30 parts, pseudomonas aeruginosa 10 parts, bacillus cereus 10 parts.
The preparation process of preparation: the raw material bacterium of cultivation is uniformly mixed by weight, then carries out cryodrying, drying temperature is 20-30 DEG C, and after dry, water content is 20%; Finally carry out packing and get final product.
Industrial application: by said preparation for the treatment of ammonia nitrogen in high density industrial sewage (NH3-N > 500mg/L), add 10 grams, preparation by every cubic metre of sewage at every turn, add 1 every day, add 10 days continuously; After one month, the clearance of the ammonia nitrogen of sewage can reach more than 99%.
Embodiment 2
Repair the preparation containing ammonia nitrogen industrial sewage, the activeconstituents of said preparation comprises the raw material bacterium of following weight part:
Thiobacillus denitrificans 100 parts, Bacillus licheniformis 80 parts, Sphingomonas 60 parts, Phanerochaete chrysosporium 50 parts, Arthrobacter globiformis 40 parts, Bacillus foecalis alkaligenes 30 parts, pseudomonas aeruginosa 10 parts, bacillus cereus 10 parts;
Wherein, thiobacillus denitrificans is ATCC25259, Bacillus licheniformis is CCTCC NO.M206082, Sphingomonas is CGMCC NO.4589, Phanerochaete chrysosporium is ATCC 24725, and Arthrobacter globiformis is CCTCC NO:M209015, and Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes ATCC 31555, pseudomonas aeruginosa is ATCC 15442, and bacillus cereus is ATCC 10876.
The preparation process of preparation: the raw material bacterium of cultivation is uniformly mixed by weight, then carries out cryodrying, drying temperature is 20-30 DEG C, and after dry, water content is 20%; Finally carry out packing and get final product.
Industrial application: by the ammonia nitrogen in high density industrial sewage (NH3-N > 500mg/L) of said preparation for the treatment of Linyi City Linshu County fertilizer plant, add 10 grams, preparation by every cubic metre of sewage at every turn, add 1 every day, add 10 days continuously; After one month, said preparation possesses the effect preferably removing ammonia nitrogen, and treatment effect is as following table 1:
Table 1
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (1)
1. repair the preparation containing ammonia nitrogen industrial sewage, said preparation is prepared from by the raw material bacterium of following weight part:
Thiobacillus denitrificans 100 parts, Bacillus licheniformis 80 parts, Sphingol single-cell 60 parts, Phanerochaete chrysosporium 50 parts, Arthrobacter globiformis 40 parts, Bacillus foecalis alkaligenes 30 parts, Pseudomonas aeruginosa 10 parts, bacillus cereus 10 parts;
Described thiobacillus denitrificans (Thiobacillus denitrificans) is ATCC25259, described Bacillus licheniformis (Bacillus licheniformis) is CCTCC NO.M206082, described Sphingol single-cell (Sphingomonas sp.) is CGMCC NO.4589, described Phanerochaete chrysosporium (Phanerochaete chrysosporium) is ATCC 24725, described Arthrobacter globiformis (Arthrobacter globiformis) is CCTCC NO:M209015, described Bacillus foecalis alkaligenes (Alcaligenes faecalis) is Bacillus foecalis alkaligenes ATCC 31555, described Pseudomonas aeruginosa (Pseudomonas aeruginosa) is pseudomonas aeruginosa ATCC 15442, described bacillus cereus (Bacillus cereus) is ATCC 10876.
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| CN103937712B (en) * | 2014-04-04 | 2016-10-05 | 华南理工大学 | A kind of Bacillus licheniformis and application thereof |
| CN104059864B (en) * | 2014-05-26 | 2016-08-24 | 绍兴柯桥江滨水处理有限公司 | One strain Bacillus cereus and preparation method thereof, application |
| CN107099483B (en) * | 2017-06-26 | 2020-09-18 | 杭州富阳佳畅机械有限公司 | Composite biological agent and application thereof in treatment of mercury-containing wastewater |
| CN108034624B (en) * | 2018-02-05 | 2021-04-27 | 厦门理工学院 | Biological agent for treating high-concentration ammonia nitrogen wastewater and preparation method thereof |
| CN110386676A (en) * | 2019-08-12 | 2019-10-29 | 东莞市台腾环保材料科技有限公司 | A kind of double tank up flow type biological filter tank systems and its method for handling organic matter |
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| CN102020359B (en) * | 2010-11-01 | 2012-07-04 | 湖南大学 | Method for removing cadmium and dichlorophenol in wastewater simultaneously by utilizing P.chrysosporium |
| CN102168054B (en) * | 2011-02-24 | 2012-12-12 | 南京大学 | Sphingomonas strain and application thereof in water treatment |
| CN102492642B (en) * | 2011-12-20 | 2013-04-17 | 江南大学 | Denitrifying strain with nitrifying function, strain-containing water body improver of multiple active microorganisms and preparation method of water body improver |
| CN102583775B (en) * | 2012-02-14 | 2013-05-15 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
| CN102864108B (en) * | 2012-09-26 | 2014-03-12 | 马小杰 | Microbial inoculum for rehabilitating nitrotoluene polluted soil and application method thereof |
| CN102864109B (en) * | 2012-09-26 | 2014-02-05 | 李丽萍 | Preparation for trinitrotoluene-contained sewage treatment and using method of preparation |
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Address after: 535000 C unit, C unit, Golden Coast, No. 52, 52 Yongfu West Avenue, Qinbei District, the Guangxi Zhuang Autonomous Region Patentee after: Yu Meixiang Address before: The two district of Shandong Province in 250011 Lixia District of Ji'nan city Zhengjue Temple District 3 Building 3 unit 603 Patentee before: Yu Meixiang |
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Address after: The two district of Shandong Province in 250011 Lixia District of Ji'nan city Zhengjue Temple District 3 Building 3 unit 603 Patentee after: Yu Meixiang Address before: 535000 C unit, C unit, Golden Coast, No. 52, 52 Yongfu West Avenue, Qinbei District, the Guangxi Zhuang Autonomous Region Patentee before: Yu Meixiang |
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Effective date of registration: 20221216 Address after: Room 515, block a, innovation building, shuangyueyuan Road, high tech Zone, Linyi City, Shandong Province Patentee after: SHANDONG TIANZHI INFORMATION TECHNOLOGY Co.,Ltd. Address before: No. 603, Unit 3, Building 3, Zone 2, Zhengjuesi Community, Lixia District, Jinan, Shandong 250011 Patentee before: Yu Meixiang |