CN104973696B - Preparation technology of biological agent for restoring industrial sewage - Google Patents
Preparation technology of biological agent for restoring industrial sewage Download PDFInfo
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- CN104973696B CN104973696B CN201510415679.5A CN201510415679A CN104973696B CN 104973696 B CN104973696 B CN 104973696B CN 201510415679 A CN201510415679 A CN 201510415679A CN 104973696 B CN104973696 B CN 104973696B
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Abstract
The invention belongs to the technical field of biological environment protection, and discloses preparation technology of a microbial agent for restoring industrial sewage. The preparation technology comprises the following steps: step 1) preparation of an inocula; step 2) preparation of a composite algae liquid; step 3) preparation of a microbial carrier; and step 4) preparation of the microbial agent. The technology is simple and available, and the prepared microbial agent can effectively restore industrial sewage.
Description
Technical field
The invention belongs to technical field of biological environmental protection, and in particular to a kind of preparation work of reparation trade effluent microorganism formulation
Skill.
Background technology
Trade effluent refers to the waste water and waste liquid of generation in industrial processes, wherein containing the industrial production for outflowing with water
The pollutant produced in materials, intermediate product, byproduct and production process.Trade effluent complicated component, it is particularly substantial amounts of
Artificial-synthetic compound enters environment, and this kind of material is mainly alkanes, olefines, alicyclic hydrocarbon type, arene, artificial synthesized
Organic matter, especially macromolecular, difficult degradation, poisonous and harmful substance such as phenol, chlorophenol, cresols, nitrophenols etc., arene,
Cyanogen class, amine and ammonia nitrogen etc. enter environment with the waste water or solid waste of plant emissions, due to answering for these this body structures of material
The strange property of polygamy and biology, can not be decomposed by the microorganisms utilization in a short time, and traditional method of wastewater treatment is dirty with activity
Mud culture acclimated microorganism can not be removed effectively to these pollutants, and these materials are accumulated in the environment for a long time,
Very big pollution is caused to the ecological environment that we depend on for existence, very big harm is brought to the physical and mental health of the mankind.
Biological prosthetic at present is that trade effluent administers very potential approach, and it mainly includes phytoremediation, microorganism
Repair and microorganism-plant combined reparation.Microorganism remediation does not produce secondary pollution due to efficiency high, is research both at home and abroad
The focus of concern.Microorganism remediation is carried out, after not only needing to obtain efficient degrading bacteria, should also possess the carrier material of function admirable
Material, current application it is more be the crop material and inorganic material crushed, when carrying out the selection of carrier material, one, carrier material
There is the performance of absorption microorganism；2nd, microorganism normal growth can be supported to breed, it is impossible to produce toxic action to microorganism；3rd,
Can be created conditions for the performance of microbial degradation function.And in order to obtain high-performance bio preparation, the reasonable compatibility of microorganism and
Carrier prepares the difficult point and emphasis for belonging to research.
The content of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of reparation trade effluent microorganism formulation
Preparation technology.The present invention adopts the following technical scheme that realization：
A kind of preparation technology for repairing trade effluent microorganism formulation, it comprises the following steps：
Step 1) prepare microbial inoculum, step 2) prepare compound algae solution, step 3) prepare microbe carrier, step 4) prepare micro- life
Thing preparation.
Specifically, comprise the following steps：
Step 1) prepare microbial inoculum：
During peanut shell and stalk put into pulverizer according to 2: 1 weight ratio, 100 mesh sieves are crossed after crushing, be combined
Powder；By bacillus megaterium, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultivated to concentration respectively7Individual/ml's
Bacterium solution, then mixes according to 2: 2: 1 volume ratio, obtains mixed bacteria liquid；By mixed bacteria liquid and composite powder according to 1: 1 weight
Than well mixed, obtain final product；
Step 2) prepare compound algae solution：
It is 1 × 10 that scenedesmus obliquus and chlorella are cultivated to concentration respectively5The algae solution of individual/ml, then according to 2: 1 volume
Than mixing, obtain final product；
Step 3) prepare microbe carrier：
(1) following each raw material for standby are taken according to weight portion, wherein, water 20-30 parts, lignite 10-12 parts, kaolin 6-8 parts,
Zeolite powder 5-7 parts, ammonium sulfate 3-5 parts, chitin 3-5 parts, silica white 1-2 parts；Preferably, the particle diameter of zeolite powder and silica white is equal
It is 200 mesh, the particle diameter of chitin is 50 mesh；
(2) lignite is crushed with disintegrating machine, is then added kaolin, be well mixed, be ground to the powder of 100 mesh,
As material 1；
(3) to the 1M calcium chloride solutions that identical weight is added in zeolite powder, stir, then 360 DEG C are calcined 3 hours,
Obtain material 2；
(4) by water, material 1, material 2, ammonium sulfate, chitin and silica white are added in stirred reactor successively, and 500
Turn/min stirring 10min, stand 30min, then 200 turns/min stirrings 3min, being finally placed in 60 DEG C of drying to moistures is
5% (parts by weight), obtain final product microbe carrier；
Step 4) prepare microorganism formulation：
Microbial inoculum, compound algae solution and microbe carrier are mixed according to 2: 1: 5 mass ratio, is stirred, 30 DEG C of dryings
It is 15% to water content, obtains final product.
Strain and algae used by the present invention can be commercially available from commercial sources such as collections.
Strain of the present invention and algae can obtain the bacterium solution or algae solution of required concentration by conventional cultural method,
This innovative point not of the invention, as space is limited, does not repeat one by one.
The beneficial effect that the present invention is obtained mainly includes：
The various strains that can form dominant microflora and algae are configured to high-performance bio system by microorganism formulation of the invention
Agent, reasonable compatibility between each microorganism, symbiosis is coordinated, mutually not antagonism, and biomass is big, and breeding is fast；Microorganism prepared by the present invention
Carrier, can not only expand the specific surface area of carrier, and with tensile strength it is big, be evenly distributed, specific surface area it is big, use the longevity
The features such as ordering long；The present invention by soak roasting etc. technique, increased the aperture number of carrier, improve surface roughness and
Specific surface area, enhances adsorption capacity；Different particle diameters is used for different material, the specific surface area of compound is increased；This
Inventing the carrier for providing can significantly improve the adhesion amount of microorganism, increase the biofilm biomass of overall attachment, micro- in reactive tank
Biological concentration is improved, and can reduce sludge yield；Anaerobism, anoxic and aerobic that the carrier that the present invention is provided is provided
The presence of various environment can promote nitration denitrification effect, while promoting the minimizing of sludge, be conducive to ammonia nitrogen in waste water
Deng the removal of pollutant.
Specific embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, below in conjunction with the application tool
Body embodiment, the technical scheme to the application is clearly and completely described, it is clear that described embodiment is only this Shen
Please a part of embodiment, rather than whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not having
There is the every other embodiment made and being obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
A kind of preparation technology for repairing trade effluent microorganism formulation, it comprises the following steps：
Step 1) prepare microbial inoculum, step 2) prepare compound algae solution, step 3) prepare microbe carrier, step 4) prepare micro- life
Thing preparation.
Specifically, comprise the following steps：
Step 1) prepare microbial inoculum：
During peanut shell and stalk put into pulverizer according to 2: 1 weight ratio, 100 mesh sieves are crossed after crushing, be combined
Powder；By bacillus megaterium, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultivated to concentration respectively7Individual/ml's
Bacterium solution, then mixes according to 2: 2: 1 volume ratio, obtains mixed bacteria liquid；By mixed bacteria liquid and composite powder according to 1: 1 weight
Than well mixed, obtain final product；
Step 2) prepare compound algae solution：
It is 1 × 10 that scenedesmus obliquus and chlorella are cultivated to concentration respectively5The algae solution of individual/ml, then according to 2: 1 volume
Than mixing, obtain final product；
Step 3) prepare microbe carrier：
(1) following each raw material for standby are taken according to weight portion, wherein, 20 parts of water, 10 parts of lignite, 6 parts of kaolin, zeolite powder 5
Part, 3 parts of ammonium sulfate, 3 parts of chitin, 1 part of silica white；Wherein, the particle diameter of zeolite powder and silica white is 200 mesh, chitin
Particle diameter is 50 mesh；
(2) lignite is crushed with disintegrating machine, is then added kaolin, be well mixed, be ground to the powder of 100 mesh,
As material 1；
(3) to the 1M calcium chloride solutions that identical weight is added in zeolite powder, stir, then 360 DEG C are calcined 3 hours,
Obtain material 2；
(4) by water, material 1, material 2, ammonium sulfate, chitin and silica white are added in stirred reactor successively, and 500
Turn/min stirring 10min, stand 30min, then 200 turns/min stirrings 3min, being finally placed in 60 DEG C of drying to moistures is
5% (parts by weight), obtain final product microbe carrier；
Step 4) prepare microorganism formulation：
Microbial inoculum, compound algae solution and microbe carrier are mixed according to 2: 1: 5 mass ratio, is stirred, 30 DEG C of dryings
It is 15% to water content, obtains final product.
Embodiment 2
A kind of preparation technology for repairing trade effluent microorganism formulation, it comprises the following steps：
Step 1) prepare microbial inoculum, step 2) prepare compound algae solution, step 3) prepare microbe carrier, step 4) prepare micro- life
Thing preparation.
Specifically, comprise the following steps：
Step 1) prepare microbial inoculum：
During peanut shell and stalk put into pulverizer according to 2: 1 weight ratio, 100 mesh sieves are crossed after crushing, be combined
Powder；By bacillus megaterium, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultivated to concentration respectively7Individual/ml's
Bacterium solution, then mixes according to 2: 2: 1 volume ratio, obtains mixed bacteria liquid；By mixed bacteria liquid and composite powder according to 1: 1 weight
Than well mixed, obtain final product；
Step 2) prepare compound algae solution：
It is 1 × 10 that scenedesmus obliquus and chlorella are cultivated to concentration respectively5The algae solution of individual/ml, then according to 2: 1 volume
Than mixing, obtain final product；
Step 3) prepare microbe carrier：
(1) following each raw material for standby are taken according to weight portion, wherein, 30 parts of water, 12 parts of lignite, 8 parts of kaolin, zeolite powder 7
Part, 5 parts of ammonium sulfate, 5 parts of chitin, 2 parts of silica white；Wherein, the particle diameter of zeolite powder and silica white is 200 mesh, chitin
Particle diameter is 50 mesh；
(2) lignite is crushed with disintegrating machine, is then added kaolin, be well mixed, be ground to the powder of 100 mesh,
As material 1；
(3) to the 1M calcium chloride solutions that identical weight is added in zeolite powder, stir, then 360 DEG C are calcined 3 hours,
Obtain material 2；
(4) by water, material 1, material 2, ammonium sulfate, chitin and silica white are added in stirred reactor successively, and 500
Turn/min stirring 10min, stand 30min, then 200 turns/min stirrings 3min, being finally placed in 60 DEG C of drying to moistures is
5% (parts by weight), obtain final product microbe carrier；
Step 4) prepare microorganism formulation：
Microbial inoculum, compound algae solution and microbe carrier are mixed according to 2: 1: 5 mass ratio, is stirred, 30 DEG C of dryings
It is 15% to water content, obtains final product.
Wherein, the bacillus megaterium is (Bacillus megatherium) CGMCC No：2267 (reference can be made to
CN101215532), Acinetobacter bauamnnii be (Acinetobacter baumanii) be ATCC 19606 (reference can be made to Infect
Immun.2012Mar；80(3)：1015-24), Sphingomonas are that (Sphingomonas sp.) CGMCC NO.4589 (can
Referring to CN102168054A), scenedesmus obliquus be (Scenedesmus obliquus) CGMCC No.8015 (reference can be made to
CN103484374A), chlorella is (Chlorella sp.) CGMCC NO：3037(CN101565675A).
Embodiment 3
The waste water dam of microorganism formulation prepared by the embodiment of the present invention 2：
Water sample is selected：Industrial wastewater with sewage treatment plant of city is as example；
Test group：Preparation prepared by the technique according to the embodiment of the present invention 2；Processing mode：Add 5g/ cubes within first day
Rice, then six days are stood, detection process the results are shown in Table 1.
Control group 1：Microbe carrier uses diatomite support；Microorganism compatibility and embodiment 2 are identical；Processing mode：Even
Continue after adding three days, it is daily 5g/ cubic metres, then six days are stood, detection process the results are shown in Table 1.
Control group 2：Microbe carrier is same as Example 2, is a difference in that, only adds three kinds of bacterium, but without algae；
Processing mode：5g/ cubic metres is added within first day, then stands six days, detection process the results are shown in Table 1.
Table 1
Conclusion：Microbe carrier of the present invention is twice the above than the specific surface area of conventional diatomite support, and microorganism is attached
Effect good, and input number of times and weight are reduced, long service life, without repeatedly dispensing, have saved cost, reduce dirt
The yield of mud.Microorganism formulation prepared by the present invention can effectively remove ammonia nitrogen in industrial wastewater, sulfide and benzene
Amine substance, is fully achieved discharge standard.
Although above having made detailed explanation to this case with generality explanation and specific embodiment, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
The modification or improvement made without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (2)
1. it is a kind of repair trade effluent microorganism formulation preparation technology, it is characterised in that the technique comprises the following steps：
Step 1) prepare microbial inoculum：
During peanut shell and stalk put into pulverizer according to 2: 1 weight ratio, 100 mesh sieves are crossed after crushing, obtain composite powder；
By bacillus megaterium, it is 1 × 10 that Acinetobacter bauamnnii and Sphingomonas are cultivated to concentration respectively7The bacterium solution of individual/ml,
Then mix according to 2: 2: 1 volume ratio, obtain mixed bacteria liquid；By mixed bacteria liquid and composite powder according to 1: 1 weight than mixed
Close uniform, obtain final product；
Step 2) prepare compound algae solution：
It is 1 × 10 that scenedesmus obliquus and chlorella are cultivated to concentration respectively5The algae solution of individual/ml, then mixes according to 2: 1 volume ratio
Close, obtain final product；
Step 3) prepare microbe carrier：
(1) following each raw material for standby are taken according to weight portion, wherein, water 20-30 parts, lignite 10-12 parts, kaolin 6-8 parts, zeolite
Powder 5-7 parts, ammonium sulfate 3-5 parts, chitin 3-5 parts, silica white 1-2 parts；
(2) lignite is crushed with disintegrating machine, is then added kaolin, be well mixed, be ground to the powder of 100 mesh, as
Material 1；
(3) to the 1M calcium chloride solutions that identical weight is added in zeolite powder, stir, then 360 DEG C are calcined 3 hours, obtain
Material 2；
(4) by water, material 1, material 2, ammonium sulfate, chitin and silica white are added in stirred reactor successively, 500 turns/
Min stirs 10min, stands 30min, then 200 turns/min stirrings 3min, and it is 5% to be finally placed in 60 DEG C of drying to moisture,
Obtain final product microbe carrier；
Step 4) prepare microorganism formulation：
Microbial inoculum, compound algae solution and microbe carrier are mixed according to 2: 1: 5 mass ratio, is stirred, 30 DEG C of dryings are to containing
Water is 15%, is obtained final product.
2. technique according to claim 1, it is characterised in that the particle diameter of the zeolite powder and silica white is 200 mesh, institute
The particle diameter for stating chitin is 50 mesh.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106587200A (en) * | 2016-12-22 | 2017-04-26 | 郑州丽福爱生物技术有限公司 | Sewage treatment agent for urban and rural polluted rivers, and preparation method thereof |
| CN108034624B (en) * | 2018-02-05 | 2021-04-27 | 厦门理工学院 | Biological agent for treating high-concentration ammonia nitrogen wastewater and preparation method thereof |
| CN108441444B (en) * | 2018-03-22 | 2020-09-25 | 中电建生态环境集团有限公司 | Compound microbial agent suitable for black and odorous water treatment |
| CN109110927B (en) * | 2018-08-24 | 2021-06-22 | 仲恺农业工程学院 | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella and acinetobacter |
| CN109231463A (en) * | 2018-09-12 | 2019-01-18 | 潘钕 | A kind of preparation and application of compound diatomite sewage-treating agent |
| CN111439845A (en) * | 2020-04-23 | 2020-07-24 | 天津市好曰子动物药业有限公司 | Composition for optimizing water body structure and preparation method thereof |
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