CN108441444B - Compound microbial agent suitable for black and odorous water treatment - Google Patents
Compound microbial agent suitable for black and odorous water treatment Download PDFInfo
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Abstract
The invention provides a compound microbial agent suitable for treatment of black and odorous water, which comprises compound bacteria and a compound enzyme preparation, wherein the compound bacteria comprise candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacillus, denitrifying bacillus, nitrosobacteria, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria, and the compound enzyme preparation comprises protease, amylase, lipase, sucrase and cellulase.
Description
Technical Field
The invention belongs to the technical field of environmental management, and particularly relates to a compound microbial agent for black and odorous water management, and a culture method and a use method thereof.
Background
The black and odorous water body is a biochemical phenomenon, when the water body suffers from serious organic pollution, the aerobic decomposition of organic matters leads the oxygen consumption rate in the water body to be greater than the reoxygenation rate, so that the water body is anoxic, the degradation of the organic matters is incomplete and the speed is reduced, odorous substances such as hydrogen sulfide, amine, ammonia, mercaptan and the like are generated in the anaerobic biodegradation process, and black and odorous substances such as FeS, MnS and the like are formed at the same time, so that the black and odorous water body is generated. The black and odorous water body is a serious water pollution phenomenon, so that the water body completely loses the use function and influences the landscape and the life and health of human beings.
At present, three methods are mainly used for treating black and odorous water. The first kind of black and odorous water body treating method is physical repair. At present, the main physical treatment methods comprise water conservancy projects such as sewage interception, water transfer, dredging and the like, mechanical algae removal, water diversion dilution, artificial flow generation and river aeration. The physical remediation has wide application range and can be used for various black and odorous water bodies, such as urban black and odorous water bodies and black and odorous water bodies in rivers and lakes. However, the treatment needs large-scale equipment and has huge engineering quantity. The second treatment method of black and odorous water is chemical remediation. Chemical remediation refers to a method for reducing or removing black and odorous substances in a water body by adding some chemical reagents into the black and odorous water body. The specific chemical repairing methods include chemical flocculation treatment technology, adding ferric salt to promote phosphorus precipitation, adding lime to denitrify and the like, and although the methods have quick effect and high efficiency, the methods are easy to cause secondary pollution. The third kind of black and odorous water is bioremediation. At present, the bioremediation technology is various and can be roughly divided into plant remediation, animal remediation and microbial remediation. The microbial remediation technology develops rapidly in recent years, and has become an effective means for solving the problem of complex environmental pollution with good economic and environmental benefits.
The composite microbial inoculum has high biological quantity, the effective bacteria number under unit mass or concentration is usually hundreds of millions, and the composite microbial inoculum can quickly become dominant functional strains in bottom mud of rivers and water bodies. Under the condition of aeration and oxygenation, functional microorganisms in the composite microbial agent can effectively promote decomposition or removal of toxic and harmful pollutants in river sediment and water, have extremely strong specificity and environmental tolerance on complex and various pollutants, can obviously improve the indigenous microbial community structure of the water, and improve the self-purification capacity of the water. However, the prior composite microbial inoculum for black and odorous water has lower biological activity and poorer treatment effect on black and odorous pollutants.
Disclosure of Invention
The invention aims to provide a compound microbial agent for treating black and odorous water, a culture method and a use method thereof, and aims to solve the problems that the existing compound microbial agent for treating black and odorous water is low in biological activity and poor in treatment effect of black and odorous pollutants.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a composite microbial agent for treating black and odorous water, which comprises composite bacteria and a composite enzyme preparation, wherein the composite bacteria comprise candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacillus, denitrifying bacillus, nitrosobacteria, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria, and the composite enzyme preparation comprises protease, amylase, lipase, sucrase and cellulase.
Preferably, the mass percentages of the strains and the complex enzyme preparation are as follows, wherein the total mass of the complex microbial agent is 100 percent: 3-5% of candida tropicalis, 3-5% of candida utilis, 3-5% of bacillus natto, 7-10% of bacillus subtilis, 5-8% of bacillus licheniformis, 8-12% of nitrobacillus, 7-10% of denitrifying bacillus, 5-12% of nitrosobacillus, 6-10% of acinetobacter, 3-5% of lactobacillus, 3-7% of aspergillus niger, 3-6% of aspergillus oryzae, 3-7% of photosynthetic bacteria and 3-6% of complex enzyme preparation.
Preferably, in the compound enzyme preparation, the protease, the amylase, the lipase, the sucrase and the cellulase are in parts by weight as follows: 6-12 parts of protease, 5-10 parts of amylase, 25-40 parts of lipase, 10-18 parts of sucrase and 10-20 parts of cellulase.
Preferably, the microbial biomass of the solid compound microbial agent reaches 1.6 × 109-2.0×109cells/g。
The invention also provides a culture method of the compound microbial agent for treating the black and odorous water body, which comprises the following steps:
providing 13 microbial strains and 5 enzymes according to the composition of the composite microbial agent for treating the black and odorous water body;
uniformly mixing each microbial strain and an enzyme component to form a compound microbial agent, mixing the compound microbial agent with a carrier, adding natural green juice, raw fish amino acid, sucrose nutrient salt and corn starch, uniformly mixing to obtain a thallus growth carrier, and naturally fermenting at the temperature of 28-35 ℃ for 3-5 days under a ventilation condition to form a loose white-gray compound microbial agent stock.
Preferably, the mass ratio of the composite microbial agent to the carrier is (15-20): (80-85), wherein the carrier is wheat bran and rice bran, and the mass ratio of the wheat bran to the rice bran in the carrier is 1: 1.
Preferably, the cell culture carrier is naturally fermented under the condition that the water content is 65-70%.
Preferably, the extraction method of the microbial strains in the compound microbial agent comprises the following steps:
and (2) screening and extracting the 13 high-efficiency microbial strains from the river sediment and the water body rich in the microorganisms, and culturing and domesticating the microbial strains to obtain the microbial strains.
The invention also provides a use method of the compound microbial agent for treating the black and odorous water body, which comprises the following steps:
providing the compound microbial agent for treating the black and odorous water body or the compound microbial agent prepared by the method;
mixing black odorous water with the compound microbial agent according to a mass ratio of 100:1, for example, 100kg of black odorous water is mixed with 1kg of compound microbial agent, and throwing the obtained mixed solution into the black odorous water body.
Preferably, in the step of throwing the mixed solution into the black and odorous water body, the throwing density of the microorganisms is 15-25g/m2。
Preferably, the microbial biomass of the solid compound microbial agent reaches 1.6 × 109-2.0×109cells/g。
The composite microbial agent for treating black and odorous water contains 13 microbial strains and 5 enzymes, and the 13 microbial strains are used as the 5 enzymesThe synergistic reaction can obviously improve the problem of stink and blackening of the water body, improve the transparency of the water body, reduce the chroma of the water body, the Chemical Oxygen Demand (COD) and the five-day Biochemical Oxygen Demand (BOD)5) Ammonia Nitrogen (NH)3-N) and Total Phosphorus (TP), decompose harmful substances such as hydrogen sulfide, cyanide, polycyclic aromatic hydrocarbon and the like, improve the ecological environment of a water body, promote aquatic plants and animals to absorb and utilize nutrient elements, and the compound microbial agent has the advantages of strong stability and specificity, synergistic symbiosis, high purification capacity, low manufacturing and operating cost, obvious effect and the like. In particular, the method comprises the following steps of,
firstly, the compound microbial agent can effectively decompose or remove organic pollutants in black and odorous water, namely Chemical Oxygen Demand (COD) and five-day Biochemical Oxygen Demand (BOD) in the water5) Ammonia Nitrogen (NH)3N) and Total Phosphorus (TP) removal rates are respectively 75-88%, 68-90%, 77-91% and 72-90%, the transparency of the water body is improved to 65cm, the odor intensity is reduced from 3-4 times to 0-1, and the chroma of the water body is reduced from 30-40 times to 2-4 times.
Secondly, the compound microbial agent can reduce the content of heavy metals to a certain extent, such as the content of copper, zinc, mercury, cadmium and lead is respectively reduced by 55-76%, 64-80%, 40-60%, 60-75% and 60-80%. Thirdly, the compound microbial agent can effectively degrade the bottom mud pollutants of the river channel and reduce the amount of the bottom mud. The principle is as follows: the river sediment mainly comprises organic matters, inorganic minerals and a mobile phase, and the content of the organic matters in the black and odorous river sediment is usually 2-10%. The sediment organic matter mainly comprises difficultly degradable humus and degradable organic matter with strong oxygen consumption function, the microorganisms remove ammonia nitrogen and oxygen consumption organic matter in the sediment and the water body through nitrification and denitrification, meanwhile, a layer of brown oxidation layer is formed on a river mud-water interface, and the sediment is reduced together through the conversion function of a benthic micro-animal food chain enriched in the sediment oxidation layer.
In addition, the compound microbial agent does not generate ecological risks, and is derived from indigenous microorganisms in the bottom mud and the water body of the river channel, so that the structures of microbial communities on two banks of the river channel, the bottom mud and the water body can be improved to a certain extent, and the environmental remediation capability of the indigenous microorganisms is promoted.
The preparation method of the compound microbial agent for treating the black and odorous water body provided by the invention is mild in condition and simple to operate, and can realize large-scale production.
The application method of the compound microbial agent for black and odorous water treatment provided by the invention only needs to mix the black and odorous water and the compound microbial agent according to a specific proportion and directly throw the mixture into the black and odorous water, does not need large-scale equipment and a large amount of labor, and can greatly reduce the cost. In addition, the method is simple and easy to operate, the biological activity of the compound microbial agent is high, and the treatment effect of black and odorous pollutants is good.
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FIG. 1 is a schematic diagram of a complex microbial inoculant provided by an embodiment of the present invention.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
The embodiment of the invention provides a compound microbial agent for treating black and odorous water, which comprises compound bacteria and a compound enzyme preparation, wherein the compound bacteria comprise candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacillus, denitrifying bacillus, nitrosobacteria, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria, and the compound enzyme preparation comprises protease, amylase, lipase, sucrase and cellulase.
The compound microbial agent for treating black and odorous water provided by the embodiment of the invention contains 13 microbial strains and 5 enzymes, and the 13 microbial strains perform synergistic reaction under the action of the 5 enzymes, so that the problem of water body smelling and blackening can be obviously improved, the transparency of the water body is improved, the chromaticity of the water body, the Chemical Oxygen Demand (COD) and the five-day Biochemical Oxygen Demand (BOD) are reduced5) Ammonia Nitrogen (NH)3-N) and Total Phosphorus (TP), decompose harmful substances such as hydrogen sulfide, cyanide, polycyclic aromatic hydrocarbon and the like, improve the ecological environment of a water body, promote aquatic plants and animals to absorb and utilize nutrient elements, and the compound microbial agent has the advantages of strong stability and specificity, synergistic symbiosis, high purification capacity, low manufacturing and operating cost, obvious effect and the like. In particular, the method comprises the following steps of,
firstly, the compound microbial agent can effectively decompose or remove organic pollutants in black and odorous water, namely Chemical Oxygen Demand (COD) and five-day Biochemical Oxygen Demand (BOD) in the water5) Ammonia Nitrogen (NH)3N) and Total Phosphorus (TP) removal rates are respectively 75-88%, 68-90%, 77-91% and 72-90%, the transparency of the water body is improved to 65cm, the odor intensity is reduced from 3-4 times to 0-1, and the chroma of the water body is reduced from 30-40 times to 2-4 times.
Secondly, the compound microbial agent can reduce the content of heavy metals to a certain extent, such as the content of copper, zinc, mercury, cadmium and lead is respectively reduced by 55-76%, 64-80%, 40-60%, 60-75% and 60-80%. Thirdly, the compound microbial agent can effectively degrade the bottom mud pollutants of the river channel and reduce the amount of the bottom mud. The principle is as follows: the river sediment mainly comprises organic matters, inorganic minerals and a mobile phase, and the content of the organic matters in the black and odorous river sediment is usually 2-10%. The sediment organic matter mainly comprises difficultly degradable humus and degradable organic matter with strong oxygen consumption function, the microorganisms remove ammonia nitrogen and oxygen consumption organic matter in the sediment and the water body through nitrification and denitrification, meanwhile, a layer of brown oxidation layer is formed on a river mud-water interface, and the sediment is reduced together through the conversion function of a benthic micro-animal food chain enriched in the sediment oxidation layer.
In addition, the compound microbial agent does not generate ecological risks, and is derived from indigenous microorganisms in the bottom mud and the water body of the river channel, so that the structures of microbial communities on two banks of the river channel, the bottom mud and the water body can be improved to a certain extent, and the environmental remediation capability of the indigenous microorganisms is promoted.
In the embodiment of the invention, 13 bacteria (candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacillus, denitrifying bacillus, nitrosobacillus, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria) in the compound microbial agent are all derived from indigenous microorganisms, so that ecological risks are not generated, the structures of microbial communities on two sides of a river channel and in a water body can be improved to a certain extent, and the environmental remediation capability of the indigenous microorganisms is improved.
The candida tropicalis can degrade phenolic compounds and lipid compounds and remove ammonia nitrogen and nitrite nitrogen;
the candida utilis can assimilate nitrate by taking urea and nitric acid as nitrogen sources;
the bacillus natto can effectively decompose organic pollutants, degrade excrement and sludge, deodorize, reduce the generation of harmful substances such as nitrite, ammonia nitrogen, hydrogen sulfide and the like, supplement beneficial bacteria in soil and inhibit the growth and the propagation of the harmful bacteria;
the bacillus subtilis has a strong inhibiting effect on harmful microorganisms such as vibrio, escherichia coli, baculovirus and the like in water, can degrade toxic and harmful substances in water, can inhibit excessive propagation of harmful algae, can eliminate bottom siltation, can improve substrate and can relieve the problem of muddy water, and the bacillus subtilis has strong activities of protease, lipase, amylase, collagenase, glucanase and cellulase;
the bacillus licheniformis can effectively remove nitrite, ammonia nitrogen, hydrogen sulfide and the like in water, decompose toxic and harmful substances, and reduce biological oxygen consumption and chemical oxygen consumption; optimizing water environment, decomposing animal residue in water, degrading feces and eliminating odor; the concentration of harmful gases is reduced, and the emission of pathogenic bacteria is reduced;
the nitrifying bacillus can convert ammonia and nitrite in soil and water into nitrate, reduce the loss of nitrogen fertilizer in soil, reduce the pollution to the environment and eliminate the harm of nitrite in water;
the denitrifying bacillus can improve the denitrifying reaction efficiency, reduce nitrite in water to generate harmless nitrogen, keep the long-term stability of the nitrification of a water system, assist other microorganisms, better adapt to the water environment, consume nitrogen nutrition, inhibit excessive propagation of algae, purify the water, inhibit the growth of pathogenic bacteria, mud skin and moss in the water and improve the substrate;
the nitrosobacteria can oxidize ammonia into nitrous acid and further oxidized into nitric acid by nitrobacteria to form nitrate which is easily absorbed by plants, the two bacteria are in mutual beneficial symbiosis to promote nitrogen circulation and decomposition and conversion of toxic and harmful substances, and meanwhile, the nitrosobacteria can also gather phosphorus;
the acinetobacter can decompose phenolic compounds and polyphosphate;
the lactobacillus can obviously reduce the content of harmful substances such as ammonia nitrogen and the like in the water body, decompose fish residual baits, excrement and organic matters, improve the water body environment, inhibit the growth of escherichia coli and the like in the water body, adjust the algae phase balance, control harmful bacteria and algae, purify the water quality and promote the healthy growth of fishes and shrimps;
the aspergillus niger can crack macromolecular organic matters and pesticides, remove heavy metals such as Fe, Zn, Cd, Cu, Ni, Pb, Mn and the like in sludge, and improve the bottom mud structure of a river channel;
the aspergillus oryzae can strongly decompose protein, cellulose, hemicellulose, lignin and the like, deodorize, eliminate plant diseases and insect pests, inhibit or kill harmful bacteria and weed seeds, decompose and mineralize elements such as carbon, nitrogen, phosphorus, potassium, sulfur and the like so as to improve soil nutrients, promote the conversion of substances and energy in soil and form and decompose humus;
the photosynthetic bacteria can utilize organic matters, sulfides, ammonia and the like in the water body as hydrogen donors and carbon sources for photosynthesis to propagate in a large quantity, so that toxic substances such as nitrite and sulfides in the water body are reduced, and the photosynthetic bacteria also have strong decomposition and conversion capacity on toxic substances such as phenol and cyanogen.
The compound microbial agent has a biodegradation effect on complex organic pollutants, a synergistic effect among microorganisms exists, aromatic compounds have complex structures, the biodegradation reaction needs participation of various enzymes, and a single microbial bacterium has all required enzymes and high activity in a small amount, so that aspergillus niger, aspergillus oryzae and the like can produce various enzyme preparations (amylase, protease, pectinase and the like) and organic acids (citric acid, gluconic acid, gallic acid and the like), and common pesticide components such as methamidophos, DDT, trichlorfon, heptachlor and the like can be degraded through an enzymatic reaction by virtue of a synergistic mechanism, so that harmless decomposition of the complex organic pollutants is accelerated; similarly, in the process of decomposing and converting ammonia nitrogen into nitrogen gas among nitrosobacteria, nitrobacteria and denitrifying bacteria, a synergistic action mechanism also exists among the three microorganisms.
Preferably, the mass percentages of the 13 strains in the compound microbial agent and the compound enzyme preparation meet the following requirements, taking the total mass of the compound microbial agent as 100 percent: 3-5% of candida tropicalis, 3-5% of candida utilis, 3-5% of bacillus natto, 7-10% of bacillus subtilis, 5-8% of bacillus licheniformis, 8-12% of nitrobacillus, 7-10% of denitrifying bacillus, 5-12% of nitrosobacillus, 6-10% of acinetobacter, 3-5% of lactobacillus, 3-7% of aspergillus niger, 3-6% of aspergillus oryzae, 3-7% of photosynthetic bacteria and 3-6% of complex enzyme preparation.
In the embodiment of the invention, the compound microbial agent also contains a compound enzyme preparation, and specifically, the compound enzyme preparation comprises protease, amylase, lipase, sucrase and cellulase. The enzyme preparation is a biochemical product which is prepared by extracting enzyme generated in organism cells or tissues by a physical or chemical method, purifying and processing the enzyme, has catalytic activity, has high efficiency and specificity, and has activity under proper conditions (pH and temperature). The catalyst is mainly used for catalyzing various chemical reactions, and has the characteristics of high catalysis efficiency, mild action conditions, energy consumption reduction, chemical pollution reduction and the like. The enzyme preparation is used in the treatment of the black and odorous water body, and can promote the decomposition of organic pollutants by fungi, and the biological nitrogen and phosphorus removal and fixation.
The protease can catalyze and decompose protein into polypeptide amino acid, such as bacillus licheniformis can produce protease, and the catalytic action of the protease provided by the compound microbial agent can improve the reaction activity;
the amylase can catalyze and decompose starch into maltose, such as bacillus subtilis, aspergillus oryzae, aspergillus niger and the like can all produce amylase, and the catalytic action of the amylase provided by the compound microbial agent can improve the reaction activity of the amylase;
the lipase can catalyze and decompose fat into glycerol and fatty acid, such as candida tropicalis has good degradation performance on high-concentration grease wastewater, and the catalytic action of the lipase provided by the compound microbial agent can improve the reaction activity of the lipase;
the sucrase can catalyze and decompose sucrose molecules into glucose and fructose, participate in photosynthesis of plants, improve carbon source and energy for growth and propagation of animals and plants, can generate sucrase for example aspergillus niger and aspergillus oryzae, and can improve the reaction activity of the sucrase under the catalysis of the composite microbial agent;
the cellulase can simultaneously catalyze and degrade cellulose macromolecules, polysaccharides, proteins and lipids to generate glucose, is a multienzyme system with a synergistic effect of a plurality of enzymes, can eliminate anti-nutritional factors and promote biological growth, can generate cellulase such as aspergillus niger and aspergillus oryzae, and can improve the reaction activity of the cellulase under the catalysis of the cellulase provided by the compound microbial agent.
Preferably, the mass percentage of 5 enzyme preparations in the compound microbial agent satisfies the following conditions, based on 100% of the total mass of the compound enzyme preparation: 6-12% of protease, 5-10% of amylase, 25-40% of lipase, 10-18% of sucrase and 10-20% of cellulase
The compound microbial agent provided by the embodiment of the invention is used for synergistically treating the water body and the bottom mud of the river, so that the source of black and odorous water body is cut off, the recovery of a water body ecological system and the formation of an ecological chain are promoted, the self-cleaning capacity of the water body is improved, and the compound microbial agent is a compound microbial agent which can be continuously developed and is used for treating black and odorous water body.
Further preferably, the microbial biomass of the solid compound microbial agent reaches 1.6 × 109-2.0×109cells/g, thereby ensuring that the black and odorous water has higher biological activity in the treatment process.
The embodiment of the invention also provides a culture method of the compound microbial agent for treating the black and odorous water body, which comprises the following steps:
s01, providing 13 microbial strains and 5 enzyme preparations according to the composition of the compound microbial preparation for treating the black and odorous water body, and carrying out directional culture and domestication on the compound microbial preparation;
s02, mixing the composite microbial strain obtained in the step S01 with an enzyme preparation component to form a composite microbial agent, uniformly mixing and stirring the composite microbial agent and a carrier (wheat bran and rice bran), adding helioviride juice, raw fish amino acid, sucrose nutrient salt and corn starch, uniformly mixing to obtain a bacterial growth carrier, and naturally fermenting at the temperature of 28-35 ℃ for 3-5 days under a ventilation condition to obtain loose white-gray composite microbial agent stock seeds.
Specifically, in the step S01, the types and contents of 13 microbial strains and 5 enzyme preparations in the composite microbial agent for treating black and odorous water, and the weights of the 13 microbial strains and 5 enzyme preparations are as described above, and are not described herein again for brevity.
Preferably, the microbial strains in the compound microbial agent are extracted from the bottom mud and the water body of a river channel, and the extraction method comprises the following steps:
extracting river sediment and water rich in microorganisms, and performing enrichment culture, separation and purification to obtain 13 microbial strains;
and culturing and domesticating the microbial strains to obtain microbial strains.
Specifically, the 13 microbial strains can be obtained by enrichment culture of different river sediment and water, or can be obtained from the same river sediment and water sample, and more than two microbial strains are enriched, and the enrichment culture is specifically determined according to the composition and content of microorganisms in the adopted river sediment and water sample.
Specifically, the microbial strains are cultured by an artificially prepared culture medium, so that the microbial strains grow and reproduce or metabolite is accumulated in a specific environment. Meanwhile, materials or matrixes of the targeted environment are gradually added into the culture medium, so that the microbial strains gradually adapt to the targeted environment to achieve the purpose of domestication, and the microbial strains can improve or change components in the targeted environment.
Preferably, the method for culturing and acclimating the microbial strain comprises:
step one, inoculating 13 microbial strains and 5 enzyme preparations in the compound microbial agent into a culture medium according to the mass percentage of the compound microbial agent, wherein the inoculation amount of the compound microbial agent in the culture medium is 5-10% (volume ratio), the culture temperature is 28-35 ℃, the rotating speed of a shaking table is 150-200r/min, the culture time is 48-72h, and the number of bacteria reaches 109And (4) obtaining the compound microbial strain with the cell/ml or more. The formula of the liquid culture medium is as follows: 8-10g of peptone, 15-16g of sucrose, 2-4g of beef extract, 6-10g of soluble starch and 0.3-0.5g of CaCl2·2H2O，2-5g NaCl，0.2-0.5g KHCO3，1.5-3g NH4Cl，0.2-0.5g NH4NO2，0.5-1g KNO3，0.2-0.4g K2HPO4，0.5-1g KH2PO4，0.04-0.1gMgSO4·7H2O，0.03-0.08g CuCl2，0.01-0.03g ZnSO4·7H2O，0.01-0.02g CoSO4·7H2Dissolving O in 1000ml of double distilled water, adjusting pH to 7.0-7.5, heating to dissolve completely, packaging into 250ml conical bottles, sealing with gauze, autoclaving at 121 deg.C for 20min, cooling, and storing in 4 deg.C refrigerator.
And step two, after the culture medium inoculated with the compound microbial agent is cultured for 48 hours, adding black odorous water every 36 hours according to the volume ratio of the culture medium to the black odorous water (from the targeted treatment environment) of 6:1-10:1 for culturing for 72-144 hours. After domestication, the stock of the compound microorganism strains of the special black and odorous water body is obtained.
In step S02, the obtained complex microbial strain and the enzyme preparation components are uniformly mixed to form a complex microbial agent, and then the complex microbial agent and the carrier are uniformly mixed and stirred. Preferably, the composite microbial agent and the carrier (wheat bran and rice bran) are uniformly mixed according to the mass ratio of (15-20) to (80-85), wherein the carrier is wheat bran and rice bran, the mass ratio of the wheat bran to the rice bran in the carrier is 1:1, namely, the wheat bran and the rice bran account for 50% respectively.
And mixing the compound microbial agent and the carrier (wheat bran and rice bran) according to the mass ratio of the compound microbial agent to the carrier (wheat bran and rice bran) of (15-20%) to (80-85%). Further, 3% of composite microbial agent, 3% of natural green juice, 3% of raw fish amino acid, 4% of sucrose nutrient salt and 1% of corn starch are added, and the mixture is uniformly mixed to obtain the thallus growth carrier.
And naturally fermenting the thallus culture carrier for 3-5 days under the conditions of proper temperature and ventilation to obtain the stock of the compound microbial agent. Preferably, the cell culture carrier is naturally fermented at 28 to 35 ℃. Preferably, the bacterial cell carrier is naturally fermented under the condition that the water content is 65-70%. The proper temperature and humidity can be favorable for the mass propagation of the strains on the carrier to form specific microbial colonies.
The loose white-grey compound microbial agent can be obtained through fermentation, and the physical diagram of the compound microbial agent is shown in figure 1.
The preparation method of the compound microbial agent for treating the black and odorous water provided by the embodiment of the invention has the advantages of mild conditions and simple operation, and can realize large-scale production.
In another aspect, the embodiment of the invention provides a use method of a compound microbial agent for black and odorous water treatment, which comprises the following steps:
providing the compound microbial agent for treating the black and odorous water body or the compound microbial agent prepared by the method;
mixing black odorous water with the compound microbial agent according to a mass ratio of 100:1, for example, 100kg of black odorous water is mixed with 1kg of compound microbial agent, and throwing the obtained mixed solution into the black odorous water body.
Preferably, in the step of throwing the mixed solution into the black and odorous water body, the throwing density of the microorganisms is 15-25g/m2。
The application method of the compound microbial agent for black and odorous water treatment provided by the embodiment of the invention only needs to mix the black and odorous water and the compound microbial agent according to a specific proportion and directly throw the mixture into the black and odorous water, does not need large-scale equipment and a large amount of labor, and can greatly reduce the cost. In addition, the method is simple and easy to implement, the biological activity of the compound microbial agent is higher, and the treatment effect of black and odorous pollutants is better.
The following description will be given with reference to specific examples.
Example 1
The compound microbial agent comprises compound bacteria and a compound enzyme preparation, wherein the compound bacteria comprise candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrifying bacillus, denitrifying bacillus, nitrosobacteria, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria, and the compound enzyme preparation comprises protease, amylase, lipase, sucrase and cellulase, wherein the total mass of the components is 100%, and the components respectively account for the following compositions in percentage by mass: 3-5% of candida tropicalis, 3-5% of candida utilis, 3-5% of bacillus natto, 7-10% of bacillus subtilis, 5-8% of bacillus licheniformis, 8-12% of nitrobacillus, 7-10% of denitrifying bacillus, 5-12% of nitrosobacillus, 6-10% of acinetobacter, 3-5% of lactobacillus, 3-7% of aspergillus niger, 3-6% of aspergillus oryzae, 3-7% of photosynthetic bacteria and 3-6% of complex enzyme preparation. The compound enzyme preparation comprises the following components in percentage by mass based on 100% of the total mass: 6-12% of protease, 5-10% of amylase, 25-40% of lipase, 10-18% of sucrase and 10-20% of cellulase.
Example 2
A culture method of a compound microbial agent for black and odorous water treatment comprises the following steps:
s11, providing 13 microbial strains and 5 enzymes according to the composition of the compound microbial agent provided in the embodiment 1;
s12, uniformly mixing and stirring the composite microbial agent and the carrier (wheat bran and rice bran) according to the mass ratio of the composite microbial agent to the carrier (wheat bran and rice bran) of 15-20% to 80-85%, wherein the mass ratio of the wheat bran to the rice bran in the carrier is 1: 1. Further, 3% of Tianhuolu juice, 3% of raw fish amino acid, 4% of sucrose nutrient salt and 1% of corn starch are added, and the thalli growth carrier is obtained after uniform mixing treatment. Naturally fermenting for 3-5 days under the conditions of ventilation, 28-35 ℃ and 65-70% of water content to obtain the compound microbial agent suitable for treating black and odorous water.
Uniformly mixing the compound microbial agent cultured in the example 2 and used for treating black odorous water according to the mass ratio of 1:100, and mixing the obtained mixed solution at the ratio of 15-25g/m2The disposal density of (2) was thrown into the black and odorous water body, and the treatment results are shown in table 1 below.
TABLE 1
| Index (I) | COD(mg/L) | BOD5(mg/L) | NH3-N(mg/L) | TP(mg/L) |
| Background value | 241 | 51.7 | 21.3 | 3.6 |
| Processing value | 33.7 | 11.4 | 2.3 | 0.43 |
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. The compound microbial agent for treating the black and odorous water is characterized by comprising compound bacteria and a compound enzyme preparation, wherein the compound bacteria comprise candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacillus, denitrifying bacillus, nitrosobacteria, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria, and the compound enzyme preparation comprises protease, amylase, lipase, sucrase and cellulase;
based on the total mass of the compound microbial agent as 100%, the mass percentage of each strain and the compound enzyme preparation are as follows: 3-5% of candida tropicalis, 3-5% of candida utilis, 3-5% of bacillus natto, 7-10% of bacillus subtilis, 5-8% of bacillus licheniformis, 8-12% of nitrobacillus, 7-10% of denitrifying bacillus, 5-12% of nitrosobacillus, 6-10% of acinetobacter, 3-5% of lactobacillus, 3-7% of aspergillus niger, 3-6% of aspergillus oryzae, 3-7% of photosynthetic bacteria and 3-6% of a complex enzyme preparation;
in the compound enzyme preparation, protease, amylase, lipase, sucrase and cellulase are in parts by weight as follows: 6-12 parts of protease, 5-10 parts of amylase, 25-40 parts of lipase, 10-18 parts of sucrase and 10-20 parts of cellulase.
2. The complex microbial inoculant according to claim 1, wherein the microbial biomass of the solid complex microbial inoculant is up to 1.6 × 109-2.0×109cells/g。
3. A culture method of a compound microbial agent for black and odorous water treatment is characterized by comprising the following steps:
providing 13 microbial strains and 5 enzymes according to the composition of the compound microbial agent for black and odorous water body treatment of claim 1 or 2;
uniformly mixing each microbial strain and an enzyme component to form a compound microbial agent, mixing the compound microbial agent with a carrier, adding natural green juice, raw fish amino acid, sucrose nutrient salt and corn starch, uniformly mixing to obtain a thallus growth carrier, and naturally fermenting at the temperature of 28-35 ℃ for 3-5 days under a ventilation condition to form a loose white-gray compound microbial agent stock.
4. The method for culturing the composite microbial inoculant for black and odorous water treatment according to claim 3, wherein the mass ratio of the composite microbial inoculant to the carrier is (15-20) to (80-85), wherein the carrier is wheat bran and rice bran, and the mass ratio of the wheat bran to the rice bran in the carrier is 1: 1.
5. The method for culturing a complex microbial inoculant for black and odorous water remediation according to claim 3 wherein the bacterial culture carrier is subjected to natural fermentation at a moisture content of 65-70%.
6. The method for culturing the complex microbial inoculant for black and odorous water treatment according to any one of claims 3 to 5, wherein the microbial strains in the complex microbial inoculant are extracted by the following steps:
and (2) screening and extracting the 13 high-efficiency microbial strains from the river sediment and the water body rich in the microorganisms, and culturing and domesticating the microbial strains to obtain the microbial strains.
7. A use method of a compound microbial agent for black and odorous water treatment is characterized by comprising the following steps:
providing the compound microbial agent for black and odorous water body treatment according to claim 1 or 2 or the compound microbial agent prepared by the method according to any one of claims 3 to 6;
mixing black odorous water with the composite microbial agent according to a mass ratio of 100:1, and throwing the obtained mixed liquid into the black odorous water body.
8. The method of using the composite microbial inoculant for black and odorous water treatment of claim 7, wherein in the step of scattering the mixed liquid into the black and odorous water, the scattering density of the microbes is 15-25g/m2。
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