CN108441444A - A kind of complex micro organism fungicide administered suitable for black and odorous water - Google Patents

A kind of complex micro organism fungicide administered suitable for black and odorous water Download PDF

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CN108441444A
CN108441444A CN201810241666.4A CN201810241666A CN108441444A CN 108441444 A CN108441444 A CN 108441444A CN 201810241666 A CN201810241666 A CN 201810241666A CN 108441444 A CN108441444 A CN 108441444A
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black
micro organism
organism fungicide
complex micro
complex
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CN108441444B (en
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郑久存
陈惠明
赵新民
邓灿
陈伟锋
徐浩
侯志强
薛信恺
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Electric Environment Control Technology Co Ltd Jianshui
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Electric Environment Control Technology Co Ltd Jianshui
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Abstract

The present invention provides a kind of complex micro organism fungicides administered suitable for black and odorous water, the complex micro organism fungicide includes compound bacteria and complex enzyme formulation, wherein, the compound bacteria includes Candida tropicalis, candida utili bacterium, bafillus natto, bacillus subtilis, bacillus licheniformis, bacterium nitrobacter, denitrification bacillus, nitrosation bacillus, acinetobacter calcoaceticus, Bacillus acidi lactici, black-koji mould, aspergillus oryzae, photosynthetic bacteria, and the complex enzyme formulation includes protease, amylase, lipase, invertase, cellulase.

Description

A kind of complex micro organism fungicide administered suitable for black and odorous water
Technical field
The invention belongs to technical field of environmental management more particularly to a kind of composite microbial bacterias administered for black and odorous water Agent and its cultural method and application method.
Background technology
Black and odorous water is a kind of Biochemical Phenomena, when water body is by serious organic contamination, the aerobic decomposition of organic matter So that oxygen consumption rate is more than reoxygenation rate, cause water hypoxia, cause organic matter degradation not exclusively, speed slow down, anaerobism Biodegradation process generates the malodoring substances such as hydrogen sulfide, amine, ammonia, mercaptan, is formed simultaneously the atraments such as FeS, MnS, makes water body Occur black smelly.Black and odorous water is serious water pollution phenomenon, and water body is made to completely lose using function, and influences landscape and the mankind Life and health.
At present for the improvement of black and odorous water, mainly there is three classes method.The administering method of first kind black and odorous water is physics It repairs.Current main physical treatment method includes that cut the hydraulic engineerings such as dirt, water transfer, dredging and mechanical algae removement, diversion dilute It releases, artificial flowing, river aeration.The application range of peripheral doses is wider, and it is black smelly to can be used for Different Waters, such as the smelly water of urban black Body and river and lake black and odorous water.But such improvement needs large scale equipment, and quantities is huge.The improvement of second class black and odorous water Method is chemical remediation.Chemical remediation refers to that some chemical reagent are added in black and odorous water, causes water body to mitigate or remove The method of black sordes matter.Specific chemical repair method has chemical flocculation treatment technology, the precipitation that molysite promotion phosphorus is added, is added Lime denitrogenation etc., these methods are although quick, efficient, but easily cause secondary pollution.The improvement of third class black and odorous water Method is biological prosthetic.Bioremediation technology at this stage is more various, is broadly divided into phytoremediation, animal reparation, micro- life Object reparation.Wherein microorganism remediation technology is quickly grown in recent years, oneself is through all good as a kind of economic benefit and environmental benefit , solve complex environment pollution problem effective means.
Biomass is high in complex micro organism fungicide, and effective bacterium number under unit mass or concentration is typically up to more than one hundred million, can be with Quickly become advantage function strain in river bottom mud and water body.Under conditions of aeration oxygen replenishing, in complex micro organism fungicide Functional microorganism can effectively facilitate the decomposition or removal of the hazardous contaminant in river bottom mud and water body, to complicated and diversified Pollutant has extremely strong specificity and environmental resistance, can be obviously improved the indigenous microorganism structure of community of water body, improves Water body self-purification ability, relative to Physical and chemical method, which has efficient, convenient, advantage of lower cost, floor space It is small, it is pollution-free the advantages that.But currently used for the composite bacteria agent of black and odorous water, bioactivity is relatively low, the processing effect of black smelly pollutant Fruit is poor.
Invention content
The purpose of the present invention is to provide a kind of complex micro organism fungicides and its cultural method administered for black and odorous water And application method, it is intended to solve the composite bacteria agent for being currently used for black and odorous water, bioactivity is relatively low, the processing effect of black smelly pollutant The poor problem of fruit.
For achieving the above object, the technical solution adopted by the present invention is as follows：
One aspect of the present invention provides a kind of complex micro organism fungicide administered for black and odorous water, the composite microbial bacteria Agent includes compound bacteria and complex enzyme formulation, wherein the compound bacteria includes Candida tropicalis, candida utili bacterium, receives Beans bacillus, bacillus subtilis, bacillus licheniformis, bacterium nitrobacter, denitrification bacillus, nitrosation bacillus, acinetobacter calcoaceticus, Bacillus acidi lactici, black-koji mould, aspergillus oryzae, photosynthetic bacteria, the complex enzyme formulation include protease, amylase, lipase, sugarcane Carbohydrase and cellulase.
Preferably, it is the quality of each bacterial strain and complex enzyme formulation in terms of 100% by the gross mass of the complex micro organism fungicide Percentage composition is as follows：Candida tropicalis 3-5%, candida utili bacterium 3-5%, bafillus natto 3-5%, withered grass bud Spore bacillus 7-10%, bacillus licheniformis 5-8%, bacterium nitrobacter 8-12%, denitrification bacillus 7-10%, nitrosation bacillus 5- 12%, acinetobacter calcoaceticus 6-10%, Bacillus acidi lactici 3-5%, black-koji mould 3-7%, aspergillus oryzae 3-6%, photosynthetic bacteria 3-7%, Complex enzyme formulation 3-6%.
Preferably, in the complex enzyme formulation, protease, amylase, lipase, invertase, cellulase mass parts Number is as follows：6-12 parts of protease, 5-10 parts of amylase, 25-40 parts of lipase, 10-18 parts of invertase, 10-20 parts of cellulase.
Preferably, the microbial biomass of the solid union microbial bacterial agent reaches 1.6 × 109-2.0×109cells/g。
Another aspect of the present invention provides a kind of cultural method for the complex micro organism fungicide administered for black and odorous water, including Following steps：
13 kinds of microbial strains and 5 kinds are provided according to the composition of the above-mentioned complex micro organism fungicide administered for black and odorous water Enzyme；
After each microbial strains and enzyme component are uniformly mixed to form complex micro organism fungicide, by the composite microbial bacteria Agent is puddled with carrier, and the green juice of day favour, raw fish amino acid, sucrose nutrition salt, cornstarch is added, and is obtained after uniform mixed processing Thalli growth carrier, spontaneous fermentation 3-5 days under 28-35 DEG C of temperature, ventilation condition form the compound micro- of loose white-grey Bacteria agent original seed.
Preferably, it is (15-20) according to the complex micro organism fungicide and carrier quality ratio:(80-85), wherein The carrier is wheat bran and rice bran, and the mass ratio of wheat bran and rice bran is 1 in the carrier:1.
Preferably, the thalline culture carrier is subjected to spontaneous fermentation under conditions of moisture is 65-70%.
Preferably, the extracting method of microbial strains is in the complex micro organism fungicide：
It screened from river bottom mud and water body rich in microorganism, extract 13 kinds of efficient microbial strains described in obtaining, After carrying out culture and acclimation to microorganism fungus kind, the microbial strains are obtained.
Further aspect of the present invention provides a kind of application method for the complex micro organism fungicide administered for black and odorous water, including Following steps：
The composite microbial that the above-mentioned complex micro organism fungicide administered for black and odorous water or the above method prepare is provided Object microbial inoculum；
It is 100 in mass ratio to take black smelly water, the black smelly water and the complex micro organism fungicide:1 is mixed, such as 100kg is black, and smelly water matches 1kg complex micro organism fungicides, and obtained mixed liquor is shed into black and odorous water.
Preferably, the mixed liquor is shed in the step into black and odorous water, the density of shedding of microorganism is 15-25g/ m2。
Preferably, the microbial biomass of the solid union microbial bacterial agent reaches 1.6 × 109-2.0×109cells/g。
Provided by the present invention for the complex micro organism fungicide that black and odorous water is administered, contain 13 kinds of microbial strains and 5 Kind of enzyme, 13 kinds of microbial strains concerted reaction under the action of 5 kinds of enzymes, carry the problem of capable of being obviously improved water body smelly nigrescence High water transparency reduces water body chroma and COD (COD), five-day BOD (BOD5), ammonia nitrogen (NH3-N) With the content of total phosphorus (TP), the harmful substances such as decomposing hydrogen sulfide, cyanide, polycyclic aromatic hydrocarbon improve water ecological setting, promote water The absorption and utilization of plant and animal to nutrient, the complex micro organism fungicide have stability and specificity stronger, association Same symbiosis, detergent power is efficient, and making is low with operating cost, takes effect the advantages that apparent.Specifically,
First, the complex micro organism fungicide effectively can decompose or remove the organic pollution in black and odorous water, water body Middle COD (COD), five-day BOD (BOD5), ammonia nitrogen (NH3- N), the removal rate of total phosphorus (TP) be respectively 75- 88%, 68-90%, 77-91%, 72-90%, water transparency are improved to 65cm, and stench intensity drops to 0-1, water from 3-4 times Body colour degree drops to 2-4 times from 30-40 times.
Secondly, the complex micro organism fungicide can reduce the content of heavy metal to a certain extent, as copper, zinc, mercury, Cadmium, lead content decline 55-76%, 64-80%, 40-60%, 60-75%, 60-80% respectively.Again, the composite microbial Object microbial inoculum can effectively degrade mercury object, reduce bed mud amount.Its principle is：River bottom mud is mainly by organic matter, nothing Machine mineral and flowing phase composition, the content of organics in black-odor riverway bed mud is usually 2-10%.Bed mud organic matter is mainly by difficulty The humus of degradation and labile organic compound composition with strong oxygen consumption effect, microorganism acted on by nitrification and denitrification, Bed mud and the ammonia nitrogen in water body and oxygen consumption organic are removed, meanwhile, in river, water sludge interface forms one layer of brown oxidation layer, passes through The bottom for being enriched in bed mud oxide layer is dwelt the transformation of microfauna food chain, cuts down bed mud jointly.
In addition, the complex micro organism fungicide not will produce ecological risk, and due to deriving from river bottom mud and water body Indigenous microorganism promote so the structure of microbiologic population in river two sides, bed mud and water body can be improved to a certain extent Into the environment remediation ability of indigenous microorganism.
Provided by the present invention for the preparation method for the complex micro organism fungicide that black and odorous water is administered, mild condition, operation Simply, large-scale production may be implemented.
Provided by the present invention for black and odorous water administer complex micro organism fungicide application method, only need to will black smelly water with The complex micro organism fungicide mixes directly shed into black and odorous water in specific proportions, does not need large scale equipment, is also not required to It is largely artificial, cost can be greatly reduced.In addition, operation is simple for this method, and the biology of complex micro organism fungicide is living Property is higher, and the treatment effect of black smelly pollutant is preferable.
Description of the drawings
Fig. 1 is the pictorial diagram of complex micro organism fungicide provided in an embodiment of the present invention.
Specific implementation mode
In order to make technical problems, technical solutions and advantageous effects to be solved by the present invention be more clearly understood, below in conjunction with Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain The present invention is not intended to limit the present invention.
In the description of the present invention, it is to be understood that, term " first ", " second " are used for description purposes only, and cannot It is interpreted as indicating or implies relative importance or implicitly indicate the quantity of indicated technical characteristic.Define as a result, " the One ", the feature of " second " can explicitly or implicitly include one or more this feature.In the description of the present invention, The meaning of " plurality " is two or more, unless otherwise specifically defined.
An embodiment of the present invention provides a kind of complex micro organism fungicide administered for black and odorous water, the complex microorganisms Microbial inoculum includes compound bacteria and complex enzyme formulation, wherein the compound bacteria include Candida tropicalis, candida utili bacterium, Bafillus natto, bacillus subtilis, bacillus licheniformis, bacterium nitrobacter, denitrification bacillus, nitrosation bacillus, non-lever Bacterium, Bacillus acidi lactici, black-koji mould, aspergillus oryzae, photosynthetic bacteria, the complex enzyme formulation include protease, amylase, fat Enzyme, invertase, cellulase.
The complex micro organism fungicide provided in an embodiment of the present invention administered for black and odorous water, contains 13 kinds of microbial strains And 5 kinds of enzymes, 13 kinds of microbial strains concerted reaction under the action of 5 kinds of enzymes can be obviously improved asking for the smelly nigrescence of water body Topic improves water transparency, reduces water body chroma and COD (COD), five-day BOD (BOD5), ammonia nitrogen (NH3- N) and total phosphorus (TP) content, the harmful substances such as decomposing hydrogen sulfide, cyanide, polycyclic aromatic hydrocarbon, improve water ecology ring Border, promotes the absorption and utilization to nutrient of water plant and animal, which has stability and single-minded Property it is stronger, cooperate with symbiosis, detergent power is efficient, make it is low with operating cost, take effect the advantages that apparent.Specifically,
First, the complex micro organism fungicide effectively can decompose or remove the organic pollution in black and odorous water, water body Middle COD (COD), five-day BOD (BOD5), ammonia nitrogen (NH3- N), the removal rate of total phosphorus (TP) be respectively 75- 88%, 68-90%, 77-91%, 72-90%, water transparency are improved to 65cm, and stench intensity drops to 0-1, water from 3-4 times Body colour degree drops to 2-4 times from 30-40 times.
Secondly, the complex micro organism fungicide can reduce the content of heavy metal to a certain extent, as copper, zinc, mercury, Cadmium, lead content decline 55-76%, 64-80%, 40-60%, 60-75%, 60-80% respectively.Again, the composite microbial Object microbial inoculum can effectively degrade mercury object, reduce bed mud amount.Its principle is：River bottom mud is mainly by organic matter, nothing Machine mineral and flowing phase composition, the content of organics in black-odor riverway bed mud is usually 2-10%.Bed mud organic matter is mainly by difficulty The humus of degradation and labile organic compound composition with strong oxygen consumption effect, microorganism acted on by nitrification and denitrification, Bed mud and the ammonia nitrogen in water body and oxygen consumption organic are removed, meanwhile, in river, water sludge interface forms one layer of brown oxidation layer, passes through The bottom for being enriched in bed mud oxide layer is dwelt the transformation of microfauna food chain, cuts down bed mud jointly.
In addition, the complex micro organism fungicide not will produce ecological risk, and due to deriving from river bottom mud and water body Indigenous microorganism promote so the structure of microbiologic population in river two sides, bed mud and water body can be improved to a certain extent Into the environment remediation ability of indigenous microorganism.
In the embodiment of the present invention, 13 kinds of bacterium (Candida tropicalis, Candida utilis ferment in the complex micro organism fungicide Female bacterium, bafillus natto, bacillus subtilis, bacillus licheniformis, bacterium nitrobacter, denitrification bacillus, nitrosation bacillus, no Lever bacterium, Bacillus acidi lactici, black-koji mould, aspergillus oryzae, photosynthetic bacteria) indigenous microorganism is both originated from, it not will produce ecology not only Risk, and the structure of microbiologic population in river two sides and water body can be improved to a certain extent, improve indigenous microorganism Environment remediation ability.
The Candida tropicalis can degrading phenol, lipoid substance, remove ammonia nitrogen and nitrite nitrogen；
The candida utili bacterium can assimilate nitrate using urea and nitric acid as nitrogen source；
The bafillus natto can effective decomposing organic pollutant, degrade excrement and sludge, deodorization reduces harmful substance The generation of nitrite, ammonia nitrogen and hydrogen sulfide etc. supplements beneficial bacterium in soil, inhibits the growth and breeding of harmful bacteria；
The bacillus subtilis has very strong suppression to harmful microorganisms such as vibrios, Escherichia coli and baculovirals in water It makes and uses, the poisonous and harmful substance in degradation water inhibits the excessive multiplication of harmful algae, eliminates bottom siltation, improves substrate, subtract Light water turbidity problem, the bacterium have the work of stronger protease, lipase, amylase, glue enzyme, dextranase, cellulase Property；
The bacillus licheniformis can effectively remove nitrite, ammonia nitrogen and hydrogen sulfide in water etc., decompose poisonous and harmful Substance reduces biological oxygen consumption and chemical oxygen consumption (COC)；Optimize water body environment, decompose the residue of animal in water, excrement of degrading is eliminated Stink；Harmful gas concentration is reduced, the discharge of pathogen is reduced；
The bacterium nitrobacter can by soil and water body ammonia and nitrite be transformed into nitrate, reduce soil nitrogenous fertilizer damage It loses, reduces the pollution to environment, eliminate the harm of water nitrite；
The denitrification bacillus can improve anti-nitration reaction efficiency, and the nitrite in reductive water is allowed to generate harmless nitrogen Gas keeps the steady in a long-term of water body system nitrification, assists other microorganisms, preferably adapts to water body environment, consumes nitrogen Nutrition inhibits algae excessive propagation, purifying water body to inhibit the growth of pathogenic bacteria, mudcake, moss in water body, improves substrate；
It is nitrous acid that the nitrosation bacillus, which can aoxidize ammonia, and then is oxidized to nitric acid by bacterium nitrobacter, is formed and is easy quilt The nitrate of plant absorption, two kinds of bacterium mutualisms promote nitrogen cycle and poisonous and harmful substance decomposition and inversion, while nitrosation bar Bacterium can also poly- phosphorus；
The acinetobacter calcoaceticus can decompose phenolic compound, poly- phosphorus；
The Bacillus acidi lactici can be substantially reduced the content of the harmful substances such as water body ammonia nitrogen, decompose fish residual bait, excrement and Organic matter improves water body environment, inhibits the growth of Escherichia coli etc. in water body, adjusts algae and balances each other, and controls and is harmful to bacterium algae, purification Water quality promotes fish and shrimp healthy growth；
The black-koji mould can crack larger molecular organics, pesticide, remove Fe, Zn in sludge, Cd, Cu, Ni, Pb, The heavy metals such as Mn, river training bed mud structure；
The aspergillus oryzae can intensive decomposition protein, cellulose, hemicellulose, lignin etc., deodorization eliminates disease pest Evil inhibits or kills harmful bacteria and weed seed, decomposes the elements such as mineralising carbon, nitrogen, phosphorus, potassium, sulphur to improve soil nutrient, promotes Matter and energy converts in soil, the formation and decomposition of humus；
The photosynthetic bacteria can be simultaneous as photosynthetic hydrogen donor using organic matter, sulfide, ammonia in water body etc. Carbon source mass propagation, to reduce the noxious materials such as the nitrite in water body, sulfide, to noxious materials such as phenol, cyanogen There is stronger decomposition and inversion ability.
The complex micro organism fungicide is to the biodegradation of complicated organic pollution, and there are the collaboration works between microorganism With, since arene compounds are complicated, the participation of a variety of enzymes of generation needs of bio-degradation reactions, and single microorganism bacterium In vivo there are enzyme needed for whole and activity and high type it is few, and black-koji mould and aspergillus oryzae etc. can produce respectively Kind enzyme preparation (amylase, protease, pectase etc.), organic acid (citric acid, gluconic acid, gallic acid etc.), for common The farm chemical ingredients such as acephatemet, DDT, metrifonate, heptachlor can rely on synergistic mechanism, degraded, accelerated by enzymatic reaction Innoxious decomposition to complicated organic pollution；Equally, such as between nitrosation bacillus, bacterium nitrobacter and denitrification bacillus Ammonia nitrogen decomposition and inversion is the process of nitrogen, and there is also synergistic effect mechanism between three kinds of microorganisms.
Preferably, it is in terms of 100% 13 in the complex micro organism fungicide by the gross mass of the complex micro organism fungicide The mass percent of kind bacterium and complex enzyme formulation meets：The Candida tropicalis 3-5%, candida utili bacterium 3-5%, Bafillus natto 3-5%, bacillus subtilis 7-10%, bacillus licheniformis 5-8%, bacterium nitrobacter 8-12%, denitrification Bacillus 7-10%, nitrosation bacillus 5-12%, acinetobacter calcoaceticus 6-10%, Bacillus acidi lactici 3-5%, black-koji mould 3-7%, aspergillus oryzae Bacterium 3-6%, photosynthetic bacteria 3-7%, complex enzyme formulation 3-6%, the complex micro organism fungicide in above-mentioned content range can not only Ensure that each microbial inoculum and enzyme preparation ingredient play its respective effect, and is conducive to each component collaboration and plays said effect.
In the embodiment of the present invention, also contain complex enzyme formulation in the complex micro organism fungicide, specifically, the complex enzyme Preparation includes protease, amylase, lipase, invertase, cellulase.Enzyme preparation is Applied Physics or chemical method, will be given birth to The enzyme generated in object cell or tissue extracts, and the manufactured still bioid with catalytic activity after purified, processing Product have high efficiency, specificity active under suitable condition (pH and temperature).It is mainly used for being catalyzed various chemistry instead It answers, has the characteristics that high catalytic efficiency, action condition are mild, reduce energy consumption, reduce chemical contamination.It is used in black and odorous water processing Enzyme preparation, the effects that mushroom can be promoted to consolidate phosphorus to the decomposition of organic pollution and biological removal of nitrogen.
It is polypeptide amino acid that protein, which can be catalytically decomposed, in the protease, and such as bacillus licheniformis can generate protease, Its reactivity can be improved in the catalytic action for the protease that complex micro organism fungicide provides；
It is maltose, such as bacillus subtilis, aspergillus oryzae and black-koji mould that starch, which can be catalytically decomposed, in the amylase Deng can generate amylase, its reactivity can be improved in the catalytic action for the amylase that complex micro organism fungicide provides；
It is glycerine and aliphatic acid that fat, which can be catalytically decomposed, in the lipase, and such as Candida tropicalis is to high-concentration grease There is waste water preferable degradation property, the catalytic action for the lipase that complex micro organism fungicide provides its reactivity can be improved；
It is glucose sugar and fructose that sucrose molecule, which can be catalytically decomposed, in the invertase, participates in the photosynthesis of plant, is planted to be dynamic The growth of object, breeding improves carbon source and energy, black-koji mould, aspergillus oryzae etc. can generate invertase, complex microorganism Its reactivity can be improved in the catalytic action for the invertase that microbial inoculum provides；
The cellulase catalytic degradation cellulose macromolecule, polysaccharide, protein and lipid can generate glucose simultaneously, be A kind of multi-enzyme system to act synergistically by multiple enzymes, can eliminate anti-nutritional factors, promote biological growth, such as aspergillus niger Bacterium, aspergillus oryzae etc. can generate cellulase, it can be improved in the catalytic action for the cellulase that complex micro organism fungicide provides Reactivity.
Preferably, it is 5 kinds of enzymes in the complex micro organism fungicide in terms of 100% by the gross mass of the complex enzyme formulation The mass percent of preparation meets：Protease 6-12%, amylase 5-10%, lipase 25-40%, invertase 10-18%, fibre The plain enzyme 10-20% of dimension
Complex micro organism fungicide provided in an embodiment of the present invention carries out collaboration improvement to water body and river bottom mud, has cut off water The black smelly root of body promotes the formation of the recovery and ecological chain of water ecology system, improves the self-purification capacity of water body, be that one kind can The complex micro organism fungicide of sustainable development administered for black and odorous water.
It is further preferred that the microbial biomass of the solid union microbial bacterial agent reaches 1.6 × 109-2.0× 109Cells/g thereby may be ensured that in black and odorous water processing procedure there is higher bioactivity.
On the other hand the embodiment of the present invention provides a kind of culture for the complex micro organism fungicide administered for black and odorous water Method includes the following steps：
S01. 13 kinds of microbial strains are provided according to the composition of the above-mentioned complex micro organism fungicide administered for black and odorous water With 5 kinds of enzyme preparations, culture and domestication are oriented to complex micro organism fungicide；
It S02., will after complex microorganism bacterial strain and enzyme preparation component that S01 is obtained being mixed to form complex micro organism fungicide The complex micro organism fungicide is uniformly puddled with carrier (wheat bran, rice bran), and the green juice of day favour, raw fish amino acid, sucrose battalion is added Salt, cornstarch are supported, obtains thalli growth carrier after uniform mixed processing, the spontaneous fermentation under 28-35 DEG C of temperature, ventilation condition 3-5 days, obtain the complex micro organism fungicide original seed of loose white-grey.
Specifically, in above-mentioned steps S01,13 kinds of microbial bacterias in the complex micro organism fungicide administered for black and odorous water The type of strain and 5 kinds of enzyme preparations, content and the weight ratio of 13 kinds of microbial strains and 5 kinds of enzyme preparations are as described above, in order to Length is saved, details are not described herein again.
Preferably, microbial strains are extracted from river bottom mud, water body and are obtained in the complex micro organism fungicide, and are extracted Method is：
River bottom mud, water body of the extraction rich in microorganism, enriched culture obtain 13 kinds of microbial bacterias after isolating and purifying Kind；
The microorganism fungus kind is cultivated, after acclimation, microbial strains are obtained.
Specifically, 13 kinds of microorganism fungus kinds can be obtained by different river bottom muds, water body enrichment culture respectively, it can also It is obtained from same river bottom mud, water body sample, is enriched with two or more microorganism fungus kinds, with specific reference to the river bottom of use Microorganism group in mud, water body sample is determined at, content.
Specifically, being cultivated microorganism fungus kind by the culture medium manually prepared, it is made to give birth under particular circumstances Long breeding or accumulation metabolite.Meanwhile be gradually added into culture medium targeting environment material or matrix, make microorganism fungus kind by Targeting environment is gradually adapted to, achievees the purpose that domestication, to enable microorganism fungus kind to improve or change the ingredient in targeting environment.
Preferably, the microorganism fungus kind is cultivated, the method for acclimation is：
Step 1, by the complex micro organism fungicide 13 kinds of microorganism fungus kinds and 5 kinds of enzyme preparations according to composite microbial The mass percent of object microbial inoculum is inoculated into culture medium, and the inoculum concentration of culture medium shared by complex micro organism fungicide is 5-10% (bodies Product ratio), cultivation temperature is 28-35 DEG C, shaking speed 150-200r/min, incubation time 48-72h, and bacterium number reaches 109Cells/ml or more obtains complex microorganism bacterial strain.The Liquid Culture based formulas is：8-10g peptones, 15-16g sugarcanes Sugar, 2-4g beef extracts, 6-10g soluble starches, 0.3-0.5gCaCl2·2H2O, 2-5g NaCl, 0.2-0.5g KHCO3, 1.5-3g NH4Cl, 0.2-0.5g NH4NO2, 0.5-1g KNO3, 0.2-0.4g K2HPO4, 0.5-1g KH2PO4, 0.04-0.1g MgSO4·7H2O, 0.03-0.08g CuCl2, 0.01-0.03g ZnSO4·7H2O, 0.01-0.02g CoSO4·7H2O, dissolving Into 1000ml double distilled waters, pH to 7.0-7.5 is adjusted, is heated to being completely dissolved, in packing to 250ml conical flasks, uses gauze Sealing, it is cooling after 121 DEG C of high pressure sterilization 20min, it is stored for future use in 4 DEG C of refrigerators.
Step 2 after the medium culture 48h after inoculating complex microorganism microbial inoculum, (is come according to culture medium and black smelly water Derived from targeting Environment control) volume ratio be 6:1-10:1 ratio is added black smelly water every 36h and is cultivated, incubation time For 72-144h.After domestication, the original seed of the complex microorganism bacterial strain of exclusive black and odorous water is obtained.
In above-mentioned steps S02, obtained complex microorganism bacterial strain and enzyme preparation component are uniformly mixed to form composite microbial After object microbial inoculum, the complex micro organism fungicide is uniformly puddled with carrier.Wherein, it is preferred that according to the composite microbial bacteria Agent and carrier quality ratio are (15-20):(80-85), wherein the carrier is wheat bran and rice bran, and wheat in the carrier The mass ratio of bran and rice bran is 1:1, i.e., 50% is respectively accounted for, the complex micro organism fungicide and carrier (wheat bran, rice bran) is uniformly mixed It mixes.
It is (15-20%) according to the complex micro organism fungicide and the carrier (wheat bran, rice bran) mass ratio:(80- 85%) ratio puddles the complex micro organism fungicide and the carrier (wheat bran, rice bran).Further, it is added compound micro- The green juice of day favour of bacteria agent quality 3%, 3% raw fish amino acid, 4% sucrose nutrition salt, 1% cornstarch, uniformly Thalli growth carrier is obtained after mixed processing.
The thalline culture carrier is subjected to spontaneous fermentation 3-5 days under preference temperature and ventilation condition, is obtained compound micro- The original seed of bacteria agent.Preferably, the thalline culture carrier is subjected to spontaneous fermentation under conditions of 28-35 DEG C.Preferably, The Bacteriophage vectors are subjected to spontaneous fermentation under conditions of moisture is 65-70%.Suitable temperature and humidity is beneficial to bacterium Strain mass propagation on carrier, forms specific microbe colony.
The fermented complex micro organism fungicide that can get loose white-grey, the pictorial diagram of the complex micro organism fungicide is such as Shown in Fig. 1.
The preparation method of the complex micro organism fungicide provided in an embodiment of the present invention administered for black and odorous water, condition temperature With, it is easy to operate, large-scale production may be implemented.
A kind of application method for the complex micro organism fungicide administered for black and odorous water of another further aspect of the embodiment of the present invention, packet Include following steps：
The composite microbial that the above-mentioned complex micro organism fungicide administered for black and odorous water or the above method prepare is provided Object microbial inoculum；
It is 100 in mass ratio to take black smelly water, the black smelly water and the complex micro organism fungicide:1 is mixed, such as 100kg is black, and smelly water matches 1kg complex micro organism fungicides, and obtained mixed liquor is shed into black and odorous water.
Preferably, the mixed liquor is shed in the step into black and odorous water, the density of shedding of microorganism is 15-25g/ m2。
The application method of the complex micro organism fungicide provided in an embodiment of the present invention administered for black and odorous water, only needing will be black Smelly water and the complex micro organism fungicide mix in specific proportions directly to be shed into black and odorous water, and large scale equipment is not needed, Also need not be largely artificial, cost can be greatly reduced.In addition, this method is simple and practicable, and the biology of complex micro organism fungicide Activity is higher, and the treatment effect of black smelly pollutant is preferable.
It is illustrated with reference to specific embodiment.
Embodiment 1
A kind of complex micro organism fungicide administered for black and odorous water, the complex micro organism fungicide include compound bacteria and answer Synthase preparation, the compound bacteria include Candida tropicalis, candida utili bacterium, bafillus natto, bacillus subtilis Bacterium, bacillus licheniformis, bacterium nitrobacter, denitrification bacillus, nitrosation bacillus, acinetobacter calcoaceticus, Bacillus acidi lactici, black-koji mould, rice Aspergillus, photosynthetic bacteria, the complex enzyme formulation include protease, amylase, lipase, invertase, cellulase, wherein The each component is 100% meter according to gross mass, respectively shared mass percentage such as following combination：Candida tropicalis 3-5%, candida utili bacterium 3-5%, bafillus natto 3-5%, bacillus subtilis 7-10%, bacillus licheniformis 5- 8%, bacterium nitrobacter 8-12%, denitrification bacillus 7-10%, nitrosation bacillus 5-12%, acinetobacter calcoaceticus 6-10%, Bacillus acidi lactici 3-5%, black-koji mould 3-7%, aspergillus oryzae 3-6%, photosynthetic bacteria 3-7%, complex enzyme formulation 3-6%.The complex enzyme system Agent component is 100% meter according to gross mass, respectively shared mass percentage such as following combination：Protease 6-12%, starch Enzyme 5-10%, lipase 25-40%, invertase 10-18%, cellulase 10-20%.
Embodiment 2
A kind of cultural method for the complex micro organism fungicide administered for black and odorous water, includes the following steps：
S11. the composition of the complex micro organism fungicide provided according to embodiment 1 provides 13 kinds of microbial strains and 5 kinds of enzymes；
S12. it is (15-20%) according to the complex micro organism fungicide and the carrier (wheat bran, rice bran) mass ratio:(80- 85%) ratio, wherein the mass ratio of wheat bran and rice bran is 1 in the carrier:1, by the complex micro organism fungicide and described Carrier (wheat bran, rice bran) is uniformly puddled.Further, the 3% green juice of day favour, 3% raw fish amino acid, 4% sucrose is added Nutritive salt, 1% cornstarch, obtain thalli growth carrier after uniform mixed processing.In ventilation, 28-35 DEG C, moisture 65- Spontaneous fermentation 3-5 days under conditions of 70% obtain being suitable for black and odorous water improvement hair complex micro organism fungicide.
The complex micro organism fungicide administered in black and odorous water that embodiment 2 is cultivated is 1 in mass ratio with black smelly water:100 Ratio uniformly mixed, by obtained mixed liquor with 15-25g/m2Density of shedding shed into black and odorous water, processing knot Fruit is as shown in table 1 below.
Table 1
Index COD(mg/L) BOD5(mg/L) NH3-N(mg/L) TP(mg/L)
Background values 241 51.7 21.3 3.6
Processing costs 33.7 11.4 2.3 0.43
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.

Claims (10)

1. a kind of complex micro organism fungicide administered for black and odorous water, which is characterized in that the complex micro organism fungicide includes Compound bacteria and complex enzyme formulation, wherein the compound bacteria includes Candida tropicalis, candida utili bacterium, natto gemma Bacillus, bacillus subtilis, bacillus licheniformis, bacterium nitrobacter, denitrification bacillus, nitrosation bacillus, acinetobacter calcoaceticus, lactic acid bar Bacterium, black-koji mould, aspergillus oryzae, photosynthetic bacteria, the complex enzyme formulation include protease, amylase, lipase, invertase, Cellulase.
2. the complex micro organism fungicide administered as described in claim 1 for black and odorous water, which is characterized in that with described compound The gross mass of microbial bacterial agent is counted for 100%, and the mass percentage of each bacterial strain and complex enzyme formulation is as follows：Candida tropicalis Bacterium 3-5%, candida utili bacterium 3-5%, bafillus natto 3-5%, bacillus subtilis 7-10%, bacillus licheniformis 5-8%, bacterium nitrobacter 8-12%, denitrification bacillus 7-10%, nitrosation bacillus 5-12%, acinetobacter calcoaceticus 6-10%, lactic acid bar Bacterium 3-5%, black-koji mould 3-7%, aspergillus oryzae 3-6%, photosynthetic bacteria 3-7%, complex enzyme formulation 3-6%.
3. the complex micro organism fungicide administered as claimed in claim 2 for black and odorous water, which is characterized in that the complex enzyme In preparation, protease, amylase, lipase, invertase, the mass fraction of cellulase are as follows：6-12 parts of protease, amylase 5-10 parts, 25-40 parts of lipase, 10-18 parts of invertase, 10-20 parts of cellulase.
4. the complex micro organism fungicide as described in any one of claims 1-3 administered for black and odorous water, which is characterized in that institute The microbial biomass for stating solid union microbial bacterial agent reaches 1.6 × 109-2.0×109cells/g。
5. a kind of cultural method for the complex micro organism fungicide administered for black and odorous water, which is characterized in that include the following steps：
It is micro- according to 13 kinds of composition offer for being used for the complex micro organism fungicide that black and odorous water is administered described in claim any one of 1-4 Biological bacterial strain and 5 kinds of enzymes；
After each microbial strains and enzyme component are uniformly mixed to form complex micro organism fungicide, by the complex micro organism fungicide with Carrier is puddled, and the green juice of day favour, raw fish amino acid, sucrose nutrition salt, cornstarch is added, and thalline is obtained after uniform mixed processing Growing carrier, spontaneous fermentation 3-5 days under 28-35 DEG C of temperature, ventilation condition form the complex microorganism of loose white-grey Microbial inoculum original seed.
6. the cultural method for the complex micro organism fungicide administered as claimed in claim 5 for black and odorous water, which is characterized in that It is (15-20) according to the complex micro organism fungicide and carrier quality ratio:(80-85), wherein the carrier be wheat bran and Rice bran, and the mass ratio of wheat bran and rice bran is 1 in the carrier:1.
7. the cultural method for the complex micro organism fungicide administered as claimed in claim 5 for black and odorous water, which is characterized in that The thalline culture carrier is subjected to spontaneous fermentation under conditions of moisture is 65-70%.
8. such as the cultural method for the complex micro organism fungicide that claim 4-7 any one of them is administered for black and odorous water, It is characterized in that, the extracting method of microbial strains is in the complex micro organism fungicide：
It screened from river bottom mud and water body rich in microorganism, extract 13 kinds of efficient microbial strains described in obtaining, to micro- After biological inoculum carries out culture and acclimation, the microbial strains are obtained.
9. a kind of application method for the complex micro organism fungicide administered for black and odorous water, which is characterized in that include the following steps：
The complex micro organism fungicide administered for black and odorous water described in claim any one of 1-4 is provided or claim 5-8 appoints The complex micro organism fungicide that one the method prepares；
It is 100 in mass ratio to take black smelly water, the black smelly water and the complex micro organism fungicide:1 is mixed, mixed by what is obtained Liquid is closed to shed into black and odorous water.
10. the application method for the complex micro organism fungicide administered as claimed in claim 9 for black and odorous water, feature exist In, the mixed liquor is shed in the step into black and odorous water, microorganism shed density be 15-25g/m2。
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