CN106222105A - A kind of process sanitary sewage complex microorganism preparations and preparation method thereof - Google Patents

A kind of process sanitary sewage complex microorganism preparations and preparation method thereof Download PDF

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CN106222105A
CN106222105A CN201610602466.8A CN201610602466A CN106222105A CN 106222105 A CN106222105 A CN 106222105A CN 201610602466 A CN201610602466 A CN 201610602466A CN 106222105 A CN106222105 A CN 106222105A
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peptone
saccharomyces cerevisiae
distilled water
accc10649
bacillus subtilis
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CN106222105B (en
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唐清池
闫青林
戴斌
郭银保
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Shenzhen Environmental Protection Technology Co., Ltd.
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唐清池
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast

Abstract

The invention belongs to microbial preparation field, be specifically related to a kind of process sanitary sewage complex microorganism preparations and preparation method thereof.Including Rhodopseudomonas palustris ACCC10649, saccharomyces cerevisiae ACCC20251, bacillus acidophilus ACCC11073, bacillus subtilis ACCC10619, Pseudomonas nitroreducens ACCC04184, acinetobacter lwoffii ACCC01091, candida tropicalis ACCC20004.The present invention is converted into simple inorganic matter by the decomposition of microorganism dirty Organic substance in water, makes sewage be efficiently treated.

Description

A kind of process sanitary sewage complex microorganism preparations and preparation method thereof
Technical field
The invention belongs to microbial preparation field, be specifically related to a kind of process sanitary sewage complex microorganism preparations and Preparation method.
Background technology
Along with human society degree improve constantly with socio-economic activity day by day frequent, problem of environmental pollution is also got over Coming the most serious, wherein the pollution of water body is especially prominent.Industrial wastewater and the discharge of sanitary sewage, be cause water pollution main Factor.Containing substantial amounts of inorganic matter and Organic substance in sanitary sewage.Inorganic matter such as chloride, sulfate, phosphate and sodium, potassium, The carbonate such as calcium, ferrum, Organic substance has cellulose, starch, fat, protein and carbamide etc..Draining into promotes in environment to swim plants Thing growth and amount reproduction, form red tide and wawter bloom.
Modern sewage disposal technology, can be divided at Physical, chemical method, physical-chemical process and biology by its action principle The big class of logos four.Owing to sewage processes without effective before entering city planting ductwork, and city planting ductwork sewage is entering sewage disposal It is not effectively treated before factory, causes sewage treatment plant's investment higher, and operating cost is huge yet.Bioremediation and tradition Processing method compare, the former has many irreplaceable advantages.Microbial bodies is small, of a great variety, and metabolism is vigorous, easily Variation, strong adaptability, effectively eliminate Organic substance, pathogen, noxious substance, remove stink, enhance the transparency, reduce colourity, Processing cost is cheap, the applicable face width to waste water quality, is not required to add dispensing agent, can avoid water quality is caused secondary pollution, be Process the ideal tools of sanitary sewage.
Summary of the invention
The present invention is to solve that in prior art, sewage disposal input cost is high, the deficiency of complex process, it is provided that at Yi Zhong Reason sanitary sewage complex microorganism preparations and preparation method thereof.
The present invention adopts the following technical scheme that realization:
A kind of process sanitary sewage complex microorganism preparations, including Rhodopseudomonas palustris (Rhodop seudanonas Palustris) ACCC10649, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis (Candida tropicalis) ACCC20004 Fermentation liquid, proportionally mix homogeneously, obtain complex micro organism fungicide.
A kind of preparation method processing sanitary sewage complex microorganism preparations, comprises the steps:
The first step, actication of culture
(1) solid medium actication of culture
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, the false unit cell of nitro reduction Bacterium (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the preservation strain of candida tropicalis (Candida tropicalis) ACCC20004, be inoculated in Inoculating needle In culture medium slant, cultivate 24-36h for 30 DEG C；
(2) fluid medium actication of culture
The preservation strain of Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, connects with Inoculating needle Planting on fluid medium, 30 DEG C of illumination cultivation 24-36h, intensity of illumination is 6000 lux；
Second step, seed fermentation
(1) oxygen consumption strain seed fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The activated spawn of (Candida tropicalis) ACCC20004 accesses in the seed culture medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h；
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the activated spawn of ACCC04184 accesses the seed culture medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649 needs illumination cultivation, illumination Intensity is 6000 lux, cultivation cycle 24-48h；
3rd step, liquid fermentation
(1) oxygen consumption strain liquid fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The seed of (Candida tropicalis) ACCC20004 accesses in the liquid fermentation medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h, obtain fermentation liquid；
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the seed of ACCC04184 accesses the liquid fermentation medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, and cultivation cycle 24-48h obtains fermentation liquid；
4th step, is mixed with complex micro organism fungicide
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the fermentation liquid of candida tropicalis (Candida tropicalis) ACCC20004, proportionally mix homogeneously, Obtain complex micro organism fungicide.
The ratio of each strain is, Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649: 10%-40%, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:10%-40%, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073:10%-40%, bacillus subtilis (Bacillus subtilis) ACCC10619:10%-40%, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184:10%- 40%, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091:10%-40%, candida tropicalis (Candida Tropicalis) ACCC20004:10%-40%.
The bacterium number of the fermentation liquid of each strain is: Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649≥5×108Cfu/mL, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251 >=5 × 106Cfu/mL, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073 >=5 × 108Cfu/mL, hay bud Spore bacillus (Bacillus subtilis) ACCC10619 >=5 × 108Cfu/mL, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) ACCC04184 >=5 × 108Cfu/mL, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091≥5×108Cfu/mL, candida tropicalis (Candida tropicalis) ACCC20004 >=5 × 106cfu/ mL。
Described in the first step, actication of culture solid medium is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:12 Brix. beerwort 1.0L, agar 15g, natural pH；Bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO4 0.02g, H2O·MnSO40.05g, CaCO320.0g, agar 15g, distilled water 1L, pH 6.8；Bacillus subtilis (Bacillus subtilis) ACCC10619: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, agar 20g, distilled water 1L, pH 7.0-7.2；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, beef Cream 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；Acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；Candida tropicalis (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, agar 15g, natural pH.
Described in the first step, actication of culture fluid medium is, Rhodopseudomonas palustris (Rhodop seudanonas Palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CaCl20.3g, distilled water 1L, pH 6.8-7.0。
Described in second step, seed fermentation oxygen consumption bacterium culture medium is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, candida tropicalis (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L are natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
Described in second step, seed fermentation anaerobic species culture medium is, Rhodopseudomonas palustris (Rhodop seudanonas Palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO4 0.5g,CcaCl20.3g, distilled water 1L, pH 6.8-7.0；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef Extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, PH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, beef Cream 10g, NaCl 5g, distilled water 1L, pH 7.0.
Described in 3rd step, liquid fermentation oxygen consumption bacterium culture medium is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, candida tropicalis (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L are natural PH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
Described in 3rd step, liquid fermentation anaerobic species culture medium is, Rhodopseudomonas palustris (Rhodop seuanonas Palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO4 0.5g,CcaCl20.3g, distilled water 1L , pH 6.8-7.0；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, cattle Meat extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, beef Cream 10g, NaCl 5g, distilled water 1L, pH 7.0.
The present invention in the effect of Guangxi Gold Han Wei bio tech ltd pilot scale is: test period is 3 days, and COD is intake For 1336mg/L, water outlet is 192mg/L, and degradation rate is 86%；Ammonia nitrogen water inlet is 331mg/L, and water outlet is 6mg/L, and degradation rate is 98%；Total phosphorus water inlet is 4.66mg/L, and water outlet is 0.15mg/L, and degradation rate is 97%；Being 2100 with colourity water inlet, water outlet is 60, Degradation rate is 97%, and test proves the present invention obvious processing effect to percolate.
The invention has the beneficial effects as follows:
1. it is not required to add dispensing agent, can avoid water quality is caused secondary pollution, be the ideal tools processing sanitary sewage；
2. microbial bodies is small, of a great variety, and metabolism is vigorous, easily makes a variation, strong adaptability, effectively eliminates Organic substance, cause of disease Body, noxious substance, remove stink, enhance the transparency, and reduces colourity；
3. processing cost is cheap, the applicable face width to waste water quality.
Detailed description of the invention
Embodiment 1: a kind of preparation method processing sanitary sewage complex microorganism preparations, comprises the steps:
The first step, actication of culture
(1) solid medium actication of culture
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, the false unit cell of nitro reduction Bacterium (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the preservation strain of candida tropicalis (Candida tropicalis) ACCC20004, be inoculated in Inoculating needle In culture medium slant, cultivate 24-36h for 30 DEG C.
The culture medium of described strain is: saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:12 Brix. beerwort 1.0L, agar 15g, natural pH；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO4 0.05g, CaCO320.0g, agar 15g, distilled water 1L, pH 6.8；Bacillus subtilis (Bacillus subtilis) ACCC10619: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, agar 20g, distilled water 1L, pH 7.0-7.2；Nitro reduces Pseudomonas (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, agar 15g, distilled water 1L, PH 7.0；Acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: peptone 5g, cattle Meat extract 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；；Candida tropicalis (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, agar 15g, natural pH.
(2) fluid medium actication of culture
The preservation strain of Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, connects with Inoculating needle Planting on fluid medium, 30 DEG C of illumination cultivation 24-36h, intensity of illumination is 6000 lux.
Described culture medium is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: ferment Female powder 3g, peptone 3g, 7H2O·MgSO40.5g, CcaCl20.3g, distilled water 1L, pH 6.8-7.0.
Second step, seed fermentation
(1) oxygen consumption strain seed fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The activated spawn of (Candida tropicalis) ACCC20004 accesses in the seed culture medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h.
The culture medium of described strain is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, torrid zone vacation Silk yeast (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: cattle Meat extract 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the activated spawn of ACCC04184 accesses the seed culture medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, cultivation cycle 24-48h.
The culture medium of described strain is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CcaCl20.3g, distilled water 1L, pH 6.8- 7.0；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, distilled water 1L, pH 7.0；
3rd step, liquid fermentation
(1) oxygen consumption strain liquid fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The seed of (Candida tropicalis) ACCC20004 accesses in the liquid fermentation medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h, obtain fermentation liquid.
The culture medium of described strain is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, torrid zone vacation Silk yeast (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: cattle Meat extract 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the seed of ACCC04184 accesses the liquid fermentation medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, and cultivation cycle 24-48h obtains fermentation liquid.
The culture medium of described strain is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO4 0.5g,CcaCl20.3g, distilled water 1L, pH 6.8- 7.0；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, distilled water 1L, pH 7.0.
4th step, is mixed with complex micro organism fungicide
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the fermentation liquid of candida tropicalis (Candida tropicalis) ACCC20004, proportionally mix homogeneously, Obtain complex micro organism fungicide.
Described ratio is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649:40%, Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:10%, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073:10%, bacillus subtilis (Bacillus subtilis) ACCC10619:10%, nitro are also Former pseudomonas (Pseudomonas nitroreducens) ACCC04184:10%, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091:10%, candida tropicalis (Candida tropicalis) ACCC20004:10%.
The bacterium number of the fermentation liquid of each described strain is: Rhodopseudomonas palustris (Rhodopseudomonas Palustris) ACCC10649 >=5 × 108Cfu/mL, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251 ≥5×106Cfu/mL, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073 >=5 × 108Cfu/mL, withered Grass bacillus cereus (Bacillus subtilis) ACCC10619 >=5 × 108Cfu/mL, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184 >=5 × 108Cfu/mL, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091 >=5 × 108Cfu/mL, candida tropicalis (Candida tropicalis) ACCC20004 >=5 ×106cfu/mL。
Embodiment 2: a kind of preparation method processing sanitary sewage complex microorganism preparations, comprises the steps:
The first step, actication of culture
(1) solid medium actication of culture
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, the false unit cell of nitro reduction Bacterium (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the preservation strain of candida tropicalis (Candida tropicalis) ACCC20004, be inoculated in Inoculating needle In culture medium slant, cultivate 24-36h for 30 DEG C.
The culture medium of described strain is.Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:12 Brix. beerwort 1.0L, agar 15g, natural pH；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO4 0.05g, CaCO320.0g, agar 15g, distilled water 1L, pH 6.8；Bacillus subtilis (Bacillus subtilis) ACCC10619: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, agar 20g, distilled water 1L, pH 7.0-7.2；Nitro reduces Pseudomonas (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；Acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: peptone 5g, cattle Meat extract 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；；Candida tropicalis (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, agar 15g, natural pH.
(2) fluid medium actication of culture
The preservation strain of Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, connects with Inoculating needle Planting on fluid medium, 30 DEG C of illumination cultivation 24-36h, intensity of illumination is 6000 lux.
Described culture medium is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: ferment Female powder 3g, peptone 3g, 7H2O·MgSO40.5g, CcaCl20.3g, distilled water 1L, pH 6.8-7.0.
Second step, seed fermentation
(1) oxygen consumption strain seed fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The activated spawn of (Candida tropicalis) ACCC20004 accesses in the seed culture medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h.
The culture medium of described strain is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, torrid zone vacation Silk yeast (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: cattle Meat extract 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the activated spawn of ACCC04184 accesses the seed culture medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, cultivation cycle 24-48h.
The culture medium of described strain is, Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO4 0.5g,CcaCl20.3g, distilled water 1L, pH 6.8- 7.0；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, distilled water 1L, pH 7.0；
3rd step, liquid fermentation
(1) oxygen consumption strain liquid fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The seed of (Candida tropicalis) ACCC20004 accesses in the liquid fermentation medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h, obtain fermentation liquid.
The culture medium of described strain is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, torrid zone vacation Silk yeast (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: cattle Meat extract 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the seed of ACCC04184 accesses the liquid fermentation medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, and cultivation cycle 24-48h obtains fermentation liquid.
The culture medium of described strain is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CaCl20.3g, distilled water 1L, pH 6.8-7.0； Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, distilled water 1L, pH 7.0.
4th step, is mixed with complex micro organism fungicide
Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the fermentation liquid of candida tropicalis (Candida tropicalis) ACCC20004, proportionally mix homogeneously, Obtain complex micro organism fungicide.
Described ratio is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649:20%, Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:10%, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073:20%, bacillus subtilis (Bacillus subtilis) ACCC10619:10%, nitro are also Former pseudomonas (Pseudomonas nitroreducens) ACCC04184:10%, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091:20%, candida tropicalis (Candida tropicalis) ACCC20004:10%.
The bacterium number of the fermentation liquid of each described strain is: Rhodopseudomonas palustris (Rhodop seudanonas Palustris) ACCC10649 >=5 × 108Cfu/mL, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251 ≥5×106Cfu/mL, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073 >=5 × 108Cfu/mL, withered Grass bacillus cereus (Bacillus subtilis) ACCC10619 >=5 × 108Cfu/mL, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184 >=5 × 108Cfu/mL, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091 >=5 × 108Cfu/mL, candida tropicalis (Candida tropicalis) ACCC20004 >=5 ×106cfu/mL。
Embodiment 3: a kind of preparation method processing sanitary sewage complex microorganism preparations, comprises the steps:
The first step, actication of culture
(1) solid medium actication of culture
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, the false unit cell of nitro reduction Bacterium (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the preservation strain of candida tropicalis (Candida tropicalis) ACCC20004, be inoculated in Inoculating needle In culture medium slant, cultivate 24-36h for 30 DEG C.
The culture medium of described strain is: saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:12 Brix. beerwort 1.0L, agar 15g, natural pH；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO4 0.05g, CaCO320.0g, agar 15g, distilled water 1L, pH 6.8；Bacillus subtilis (Bacillus subtilis) ACCC10619: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, agar 20g, distilled water 1L, pH 7.0-7.2；Nitro reduces Pseudomonas (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；Acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: peptone 5g, cattle Meat extract 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；；Candida tropicalis (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, agar 15g, natural pH.
(2) fluid medium actication of culture
The preservation strain of Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, connects with Inoculating needle Planting on fluid medium, 30 DEG C of illumination cultivation 24-36h, intensity of illumination is 6000 lux.
Described culture medium is, Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649: Yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CaCl20.3g, distilled water 1L, pH 6.8-7.0；
Second step, seed fermentation
(1) oxygen consumption strain seed fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The activated spawn of (Candida tropicalis) ACCC20004 accesses in the seed culture medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h.
The culture medium of described strain is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, torrid zone vacation Silk yeast (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: cattle Meat extract 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the activated spawn of ACCC04184 accesses the seed culture medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, cultivation cycle 24-48h.
The culture medium of described strain is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CaCl20.3g, distilled water 1L pH 6.8-7.0； Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, distilled water 1L, pH 7.0.
3rd step, liquid fermentation
(1) oxygen consumption strain liquid fermentation
Saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus subtilis (Bacillus Subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, candida tropicalis The seed of (Candida tropicalis) ACCC20004 accesses in the liquid fermentation medium of each strain, inoculum concentration 3%- 5%, cultivation temperature 30 DEG C, mixing speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h, obtain fermentation liquid.
The culture medium of described strain is, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, torrid zone vacation Silk yeast (Candida tropicalis) ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis (Bacillus subtilis) ACCC10619, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091: cattle Meat extract 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2；
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, Pseudomonas nitroreducens (Pseudomonas Nitroreducens) during the seed of ACCC04184 accesses the liquid fermentation medium of each strain, inoculum concentration 3%-5%, cultivate Temperature 30 DEG C, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649 needs illumination cultivation, and illumination is strong Degree is 6000 lux, and cultivation cycle 24-48h obtains fermentation liquid.
The culture medium of described strain is, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CaCl20.3g, distilled water 1L, pH 6.8- 7.0；Bacillus acidophilus (Lactobacillus acidophilus) ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, distilled water 1L, pH 7.0.
4th step, is mixed with complex micro organism fungicide
Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073, bacillus subtilis (Bacillus subtilis) ACCC10619, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184, acinetobacter lwoffii (Acinetobacter lwoffii) ACCC01091, the fermentation liquid of candida tropicalis (Candida tropicalis) ACCC20004, proportionally mix homogeneously, Obtain complex micro organism fungicide.
Described ratio is, Rhodopseudomonas palustris (Rhodop seudanonas palustris) ACCC10649: 20%, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251:20%, bacillus acidophilus (Lactobacillus Acidophilus) ACCC11073:10%, bacillus subtilis (Bacillus subtilis) ACCC10619:10%, nitro are also Former pseudomonas (Pseudomonas nitroreducens) ACCC04184:10%, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091:10%%, candida tropicalis (Candida tropicalis) ACCC20004:20%.
The bacterium number of the fermentation liquid of each described strain is: Rhodopseudomonas palustris (Rhodop seudanonas Palustris) ACCC10649 >=5 × 108Cfu/mL, saccharomyces cerevisiae (Saccharomyces cerevisiae) ACCC20251 ≥5×106Cfu/mL, bacillus acidophilus (Lactobacillus acidophilus) ACCC11073 >=5 × 108Cfu/mL, withered Grass bacillus cereus (Bacillus subtilis) ACCC10619 >=5 × 108Cfu/mL, Pseudomonas nitroreducens (Pseudomonas nitroreducens) ACCC04184 >=5 × 108Cfu/mL, acinetobacter lwoffii (Acinetobacter Lwoffii) ACCC01091 >=5 × 108Cfu/mL, candida tropicalis (Candida tropicalis) ACCC20004 >=5 ×106cfu/mL。

Claims (10)

1. one kind processes sanitary sewage complex microorganism preparations, it is characterised in that: include Rhodopseudomonas palustris ACCC10649, saccharomyces cerevisiae ACCC20251, bacillus acidophilus ACCC11073, bacillus subtilis ACCC10619, nitro are also Former pseudomonas ACCC04184, acinetobacter lwoffii ACCC01091, the fermentation liquid of candida tropicalis ACCC20004, according to Ratio mix homogeneously, obtains complex micro organism fungicide.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 1, its feature exists In: comprise the steps:
The first step, actication of culture
(1) solid medium actication of culture
The reduction of saccharomyces cerevisiae ACCC20251, bacillus acidophilus ACCC11073, bacillus subtilis ACCC10619, nitro is false single Born of the same parents bacterium ACCC04184, acinetobacter lwoffii ACCC01091, the preservation strain of candida tropicalis ACCC20004, use Inoculating needle It is inoculated in culture medium slant, cultivates 24-36h for 30 DEG C；
(2) fluid medium actication of culture
The preservation strain of Rhodopseudomonas palustris ACCC10649, is inoculated on fluid medium with Inoculating needle, 30 DEG C of illumination cultivation 24-36h, intensity of illumination is 6000 lux；
Second step, seed fermentation
(1) oxygen consumption strain seed fermentation
Saccharomyces cerevisiae ACCC20251, bacillus subtilis ACCC10619, acinetobacter lwoffii ACCC01091, the false silk ferment in the torrid zone The activated spawn of female ACCC20004 accesses in the seed culture medium of each strain, inoculum concentration 3%-5%, cultivation temperature 30 DEG C, stirring Speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h；
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris ACCC10649, bacillus acidophilus ACCC11073, the work of Pseudomonas nitroreducens ACCC04184 Change in the seed culture medium that strain accesses each strain, inoculum concentration 3%-5%, cultivation temperature 30 DEG C, Rhodopseudomonas palustris ACCC10649 needs illumination cultivation, and intensity of illumination is 6000 lux, cultivation cycle 24-48h；
3rd step, liquid fermentation
(1) oxygen consumption strain liquid fermentation
Saccharomyces cerevisiae ACCC20251, bacillus subtilis ACCC10619, acinetobacter lwoffii ACCC01091, the false silk ferment in the torrid zone The seed of female ACCC20004 accesses in the liquid fermentation medium of each strain, inoculum concentration 3%-5%, cultivation temperature 30 DEG C, stirring Speed 130-180r/min, ventilation 0.5m3/ h, cultivation cycle 24-48h, obtain fermentation liquid；
(2) anaerobic species seed fermentation
Rhodopseudomonas palustris ACCC10649, bacillus acidophilus ACCC11073, the kind of Pseudomonas nitroreducens ACCC04184 Son accesses in the liquid fermentation medium of each strain, inoculum concentration 3%-5%, cultivation temperature 30 DEG C, Rhodopseudomonas palustris ACCC10649 needs illumination cultivation, and intensity of illumination is 6000 lux, and cultivation cycle 24-48h obtains fermentation liquid；
4th step, is mixed with complex micro organism fungicide
Rhodopseudomonas palustris ACCC10649, saccharomyces cerevisiae ACCC20251, bacillus acidophilus ACCC11073, bacillus subtilis Bacterium ACCC10619, Pseudomonas nitroreducens ACCC04184, acinetobacter lwoffii ACCC01091, candida tropicalis The fermentation liquid of ACCC20004, proportionally mix homogeneously, obtain complex micro organism fungicide.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: the ratio of each strain is, Rhodopseudomonas palustris ACCC10649:10%-40%, saccharomyces cerevisiae ACCC20251:10%-40%, Bacillus acidophilus ACCC11073:10%-40%, bacillus subtilis ACCC10619:10%-40%, Pseudomonas nitroreducens ACCC04184:10%-40%, acinetobacter lwoffii ACCC01091:10%-40%, candida tropicalis ACCC20004:10%- 40%。
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: the bacterium number of the fermentation liquid of each strain is: Rhodopseudomonas palustris ACCC10649 >=5 × 108Cfu/mL, saccharomyces cerevisiae ACCC20251≥5×106Cfu/mL, bacillus acidophilus ACCC11073 >=5 × 108Cfu/mL, bacillus subtilis ACCC10619≥5×108Cfu/mL, Pseudomonas nitroreducens ACCC04184 >=5 × 108Cfu/mL, acinetobacter lwoffii ACCC01091≥5×108Cfu/mL, candida tropicalis ACCC20004 >=5 × 106cfu/mL。
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: described in the first step, actication of culture solid medium is, saccharomyces cerevisiae ACCC20251:12 Brix. beerwort 1.0L, agar 15g, natural pH；Bacillus acidophilus ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, Glucose 5.0g, sodium acetate 5.0g, citric acid diamino 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O· MgSO40.02g, H2O·MnSO40.05g, CaCO320.0g, agar 15g, distilled water 1L, pH 6.8；Bacillus subtilis ACCC10619: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, agar 20g, distilled water 1L, pH 7.0-7.2；Nitro reduces Pseudomonas ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；Lu Shi is not Lever bacterium ACCC01091: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, agar 15g, distilled water 1L, pH 7.0；The false silk in the torrid zone Yeast ACCC20004:12 Brix. beerwort 1.0L, agar 15g, natural pH.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: described in the first step, actication of culture fluid medium is, Rhodopseudomonas palustris ACCC10649: yeast powder 3g, peptone 3g, 7H2O·MgSO40.5g, CaCl20.3g, distilled water 1L, pH 6.8-7.0.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: described in second step, seed fermentation oxygen consumption bacterium culture medium is, saccharomyces cerevisiae ACCC20251, candida tropicalis ACCC20004:12 Brix. beerwort 1.0L, natural PH；Bacillus subtilis ACCC10619, acinetobacter lwoffii ACCC01091: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: described in second step, seed fermentation anaerobic species culture medium is, Rhodopseudomonas palustris ACCC10649: yeast powder 3g, albumen Peptone 3g, 7H2O·MgSO40.5g, CcaCl20.3g, distilled water 1L pH 6.8-7.0；Bacillus acidophilus ACCC11073: cheese Peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid two Ammonia 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO3 20.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, Distilled water 1L, pH 7.0.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: described in the 3rd step, liquid fermentation oxygen consumption bacterium culture medium is, saccharomyces cerevisiae ACCC20251, candida tropicalis ACCC20004:12 Brix. beerwort 1.0L, natural pH；Bacillus subtilis ACCC10619, acinetobacter lwoffii ACCC01091: Carnis Bovis seu Bubali cream 10g, peptone 10g, NaCl 5g, distilled water 1L, pH 7.0-7.2.
A kind of preparation method processing sanitary sewage complex microorganism preparations the most according to claim 2, its feature exists In: described in the 3rd step, liquid fermentation anaerobic species culture medium is, Rhodopseudomonas palustris ACCC10649: yeast powder 3g, albumen Peptone 3g, 7H2O·MgSO40.5g, CcaCl20.3g, distilled water 1L, pH 6.8-7.0；Bacillus acidophilus ACCC11073: cheese Peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid two Ammonia 2.0g, tween Tween80 1.0g, K2HPO42.0g, 7H2O·MgSO40.02g, H2O·MnSO40.05g, CaCO3 20.0g, distilled water 1L, pH 6.8；Pseudomonas nitroreducens ACCC04184: peptone 5g, Carnis Bovis seu Bubali cream 10g, NaCl 5g, Distilled water 1L, pH 7.0.
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CN107136127A (en) * 2017-03-23 2017-09-08 北京百丰天下生物科技有限公司 Salt tolerant composite probiotics preparations and preparation method thereof
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