CN109971682A - A kind of EM bacterium solution for aquaculture and preparation method thereof and application - Google Patents
A kind of EM bacterium solution for aquaculture and preparation method thereof and application Download PDFInfo
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- CN109971682A CN109971682A CN201910257971.7A CN201910257971A CN109971682A CN 109971682 A CN109971682 A CN 109971682A CN 201910257971 A CN201910257971 A CN 201910257971A CN 109971682 A CN109971682 A CN 109971682A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention relates to a kind of EM bacterium solutions for aquaculture and preparation method thereof and application, the EM bacterium solution the preparation method comprises the following steps: according to parts by weight, it takes FTR-1,1-3 parts of 1-3 parts of lactobacillus acidophilus FTR-3,1-3 parts of streptococcus fecalis of FTR-2,2-4 parts of lactobacillus plantarum FTY-10,0.5-1 parts of bacillus coagulans of FTJ-1,0.5-1 parts of saccharomyces cerevisiae clostridium butyricum FTD-1 to mix, it is spare that strain is made；Gained strain 0.1-0.5% by weight percentage is inoculated into culture medium, inoculation mixed liquor is poured into fermentation cylinder for fermentation, EM bacterium solution finished product is made.The technical program utilizes physio-biochemical characteristics, the metabolic pathway difference of a variety of probiotics, water pollutant is quickly removed by the growth metabolism of various probiotics, inhibit harmful growth of pathogenic bacteria, promotes beneficial bacterium and the growth and breeding beneficial to algae in water, keep the good algae phase of water body and bacterium phase；The pollutant in water body is effectively decomposed simultaneously, and quick improvement water quality improves the immunity of aquatic livestock, promotes the growth of aquatic livestock, has the characteristics that ecological, environmental protective, effect is lasting.
Description
Technical field
The present invention relates to microbial fermentation culture technique field more particularly to a kind of EM bacterium solution for aquaculture and its
Production method and application.
Background technique
Microorganism is widely present in nature, is widely used in food, medical treatment, health care, herding, environmental protection, kind at present
In plant and aquaculture.Environmental protection, soil improvement, holding host health etc. are played an important role, therefore referred to as EM
Bacterium (effective microorganism).EM bacterium is the general name of a big quasi-microorganism, including bacillus, lactic acid bacteria, yeast
Bacterium etc. can be effectively improved water body environment, improve animal and plant disease resisting ability, promote crop, livestock and poultry, aquatic livestock growth.Simultaneously
It can also be applied to the fields such as livestock and poultry farm deodorization, refuse landfill deodorization.
EM bacterium wide variety, different manufacturers, the strain combination used is different, and training method is also different, production
Product effect is also different.EM bacterium function is so more, the strain selection of EM bacterium, culture medium prescription, culture process, application method etc.
The effect of being directly related to EM bacterium.
It is currently applied to the EM bacterium multiplicity of aquaculture, also irregular in effect, some prices are expensive.
CN106508761A provides a kind of method using the pool cultivated Penaeus Vannmei of probiotics cement, and this method requires complicated
Harsh use condition, all requires water Inversion phenomenon, the pH of breeding water body, improves using threshold.CN 108587966A
Provide a kind of application method of pure plant source EM bacterium culture medium and its fermented product in aquaculture, this method manufacture
Technique is cumbersome, and culture medium is expensive, improves application cost.104082345 A of CN provides a kind of dedicated EM bacterium powder of aquaculture
And its preparation method and application, this method incubation time is long, first carries out solid fermentation, dries pulverizing is worth into after fermentation
Product, complex manufacturing technology.
Summary of the invention
In order to overcome the above-mentioned deficiency of the prior art, the present invention provides a kind of EM bacterium solutions and its system for aquaculture
Make methods and applications, water pollutant is quickly removed by the growth metabolism of various probiotics, inhibits harmful growth of pathogenic bacteria, promote
Beneficial bacterium and the growth and breeding beneficial to algae in water inlet, keep the good algae phase of water body and bacterium phase.
To achieve the goals above, the present invention is achieved by the following technical solutions:
The first purpose of this invention is to propose a kind of EM bacterium solution for aquaculture, includes following opportunistic pathogen kind: thermophilic
Lactobacillus lactis FTR-1, lactobacillus plantarum FTR-2, streptococcus fecalis FTR-3, saccharomyces cerevisiae FTJ-1, bacillus coagulans FTY-10,
Clostridium butyricum FTD-1；The EM bacterium solution is prepared as follows to obtain: according to parts by weight, taking 1-3 parts of lactobacillus acidophilus
FTR-1,1-3 parts of FTJ-1,0.5-1 parts of saccharomyces cerevisiae of FTR-3,1-3 parts of streptococcus fecalis of FTR-2,2-4 parts of lactobacillus plantarum condensations
FTY-10,0.5-1 parts of clostridium butyricum FTD-1 mixing of bacillus, it is spare to be made EM strain；By gained EM strain percentage by weight
It is inoculated into culture medium than 0.1-0.5%, inoculation mixed liquor is poured into fermentation cylinder for fermentation, EM bacterium solution finished product is made.
Further, the culture medium be comprising 15-20g/L molasses, 5-10g/L ammonium sulfate, 0.5-1.5g/L magnesium sulfate,
The aqueous solution of 1-3g/L potassium dihydrogen phosphate, 1-3g/L dipotassium hydrogen phosphate.
Further, the culture medium is placed at 115 DEG C the 20min that sterilizes after the completion of preparation, spare after cooling.
Further, each opportunistic pathogen kind concentration are as follows: lactobacillus acidophilus 100,000,000,000/gram, lactobacillus plantarum 100,000,000,000/gram, excrement chain
Coccus 40,000,000,000/gram, saccharomyces cerevisiae 20,000,000,000/gram, bacillus coagulans 20,000,000,000/gram, clostridium butyricum 20,000,000,000/gram.
Further, the fermentation condition be 28-32 DEG C sealing and fermenting 4-6 days.
Second object of the present invention is to propose that any of the above-described EM bacterium solution for aquaculture is adjusting water
Produce the application in breeding water body.
Third object of the present invention is to propose the user of any of the above-described EM bacterium solution for aquaculture
Method, method particularly includes: the dosage of EM bacterium solution is calculated in per acre/rice water surface 2-3Kg ratio, it is dilute with 50-100 times of volume clear water
It releases, Direct Uniform is splashed in water body, and water temperature is at 15 DEG C or more.
Fourth object of the present invention is to propose the user of any of the above-described EM bacterium solution for aquaculture
Method, method particularly includes: EM bacterium solution is directly mixed with aquatic feeds in the ratio of 1-3wt%, is then fed.
The microbial inoculum is applied to adjust water quality in aquaculture, in each component:
Lactobacillus acidophilus acid producing ability is strong, can decompose the small-molecule substances such as carbohydrate rapidly, produces the acid such as a large amount of lactic acid of acid
Property substance, can maintain water body pH stablize.Lactobacillus acidophilus is adjustable the balance of microbial flora simultaneously, enhances immunity of organism
Power；Lactobacillus plantarum can secrete a large amount of ectoenzyme, can the polluters such as protein, carbohydrate, fat in fast decoupled water body,
Ammonia nitrogen, The sub-salt are quickly reduced, is purified water.Secondly lactobacillus plantarum and its metabolite contain higher superoxide dismutase
Enzyme, can scavenging activated oxygen, enhance organism immunity of aquatic livestock, promote aquatic livestock fast-growth；Streptococcus fecalis can be with
A large amount of antibacterial substance is secreted, the growth of some pathogenic microorganisms can be inhibited, improves the immunity of aquatic livestock.Excrement chain simultaneously
Coccus can also generate the nutriments such as lactic acid, amino acid, vitamin during growth, promote the growth of aquatic livestock；It makes
Brewer yeast can secrete a large amount of ectoenzyme, can decompose the substances such as starch, the cellulose in water body rapidly, be translated into small point
Sub- substance allows algae, mushroom and the zooplankter in water body to be easy to absorb, promotes the life of Measures of Algae in Water Body mushroom and zooplankter
It is long, sufficient natural bait is provided for aquatic livestock；Bacillus coagulans secrete the enzyme systems such as a large amount of amylase, protease, fastly
Speed decomposes the polluters such as organic matter, the ammonia nitrogen in water body, purifies water；Bacillus coagulans during growth can be with
The antibacterial materials such as lipopeptid, polypeptide are generated, inhibits the growth of some pathogens in water body, improves the immunity of aquatic livestock；Butyric acid
Clostridium is Gram-positive anaerobic spore-bearing bacilli, and clostridium butyricum can secrete oligosaccharidase, polysaccharide is degraded to oligosaccharide, double
The intestinal beneficial bacteriums such as discrimination bacillus, lactic acid bacteria, enterococcus faecalis are utilized, conducive to the digestion and absorption of protein, fat in feed, thus
Promote the growth of beneficial bacterium, adjusts aquatic livestock intestinal eubiosis.Clostridium butyricum can make carbohydrate fermentation form a large amount of butyric acid, vinegar
Acid and short chain fatty acids, these short chain fatty acids such as lactic acid can maintain acidic environment, reduce the pH value of enteron aisle, prevent pathogen and
Spoilage organisms abnormality proliferation in enteron aisle reduces enterotoxin, promotes the growth of aquatic livestock, improve the immunity of aquatic livestock.
Therefore, compared with prior art, the beneficial effects of the present invention are:
(1) technical solution of the present invention utilizes lactobacillus acidophilus, lactobacillus plantarum, streptococcus fecalis, saccharomyces cerevisiae, condensation bud
The physio-biochemical characteristics of spore bacillus and clostridium butyricum, metabolic pathway difference, are quickly removed by the growth metabolism of various probiotics
Water pollutant inhibits harmful growth of pathogenic bacteria, promotes beneficial bacterium and the growth and breeding beneficial to algae in water, keeps the good algae of water body
Phase and bacterium phase；The pollutant in water body is effectively decomposed simultaneously, and quick improvement water quality improves the immunity of aquatic livestock, promotes water
Produce the growth of animal.Have the characteristics that ecological, environmental protective, effect is lasting.
(2) EM bacterium solution raw material of the invention acts synergistically science, has given full play to the characteristic and function of various bacterium, when use
It is easy to operate, equipment investment is few, raw material can inexpensively obtain, and is suitble to scale industrial production, is also suitble to the production of individual workship, family, can be with
Save a large amount of aquaculture costs.
Specific embodiment
It shows that example illustrates certain embodiments of the present invention, and should not be construed as limiting model of the invention
It encloses.Present disclosure can be improved from material, method and reaction condition simultaneously, all these improvement should all
It falls within spirit and scope of the invention.
The invention discloses a kind of EM bacterium solutions for aquaculture, include following opportunistic pathogen kind: lactobacillus acidophilus FTR-1,
Lactobacillus plantarum FTR-2, streptococcus fecalis FTR-3, saccharomyces cerevisiae FTJ-1, bacillus coagulans FTY-10, clostridium butyricum FTD-1；
The EM bacterium solution is prepared as follows to obtain: according to parts by weight, taking FTR-1,1-3 portions of plants of 1-3 parts of lactobacillus acidophilus
FTJ-1,0.5-1 parts of bacillus coagulans FTY-10 of FTR-2,2-4 parts of lactobacillus FTR-3,1-3 parts of streptococcus fecalis saccharomyces cerevisiae,
0.5-1 parts of clostridium butyricum FTD-1 mixing, it is spare to be made EM strain；Gained EM strain 0.1-0.5% by weight percentage is inoculated with
Into culture medium, inoculation mixed liquor is poured into fermentation cylinder for fermentation, EM bacterium solution finished product is made.
Preferably, culture medium is to include 15-20g/L molasses, 5-10g/L ammonium sulfate, 0.5-1.5g/L magnesium sulfate, 1-3g/L
The aqueous solution of potassium dihydrogen phosphate, 1-3g/L dipotassium hydrogen phosphate.
Preferably, culture medium is placed at 115 DEG C the 20min that sterilizes after the completion of preparation, spare after cooling.
Preferably, fermentation condition be 28-32 DEG C sealing and fermenting 4-6 days.
The invention also discloses the above-mentioned EM bacterium solutions for aquaculture to adjust the application in aquaculture system.Specifically
There are two types of application methods, is respectively as follows:
(1) dosage of EM bacterium solution is calculated in per acre/rice water surface 2-3Kg ratio, is diluted with 50-100 times of volume clear water,
Direct Uniform is splashed in water body, and water temperature is at 15 DEG C or more.
(2) EM bacterium solution is directly mixed with aquatic feeds in the ratio of 1-3wt%, is then fed.
Illustrate below by way of specific embodiment, bacterial strain involved in embodiment is the limited public affairs of Wuhan Feng Tian biotechnology
Department is isolated from natural environment, and is subject to preservation, commercially available；Bacterium raw material involved in embodiment is Wuhan Feng Tiansheng
The microbial germ powder that object Science and Technology Ltd. sells；Preparation in embodiment 1-4 is to be compounded by a variety of bacterium powder and auxiliary material
Pulvis.
Embodiment 1:
1, the preparation of EM bacterium solution
(1) fluid nutrient medium is prepared
By concentration calculation: molasses 15g/L, ammonium sulfate 5g/L, magnesium sulfate 1g/L, potassium dihydrogen phosphate 2g/L, dipotassium hydrogen phosphate
1g/L, remaining is water.After the completion of preparation, the 20min that sterilizes is placed at 115 DEG C, it is spare after cooling.
(2) it is inoculated with
It takes lactobacillus acidophilus FTR-1 bacterium powder 1g, lactobacillus plantarum FTR-2 bacterium powder 1g, streptococcus fecalis FTR-3 bacterium powder 3g, make
Brewer yeast FTJ-1 bacterium powder 3g, 0 0.5g of bacillus coagulans FTY-1 bacterium powder, clostridium butyricum FTD-1 bacterium powder 0.75g mixing, are made
EM strain is spare；Strain is inoculated into the culture medium in 9.25Kg step (1).Wherein bacterium powder lactobacillus acidophilus original powder concentration is
100000000000/gram, lactobacillus plantarum original powder concentration is 100,000,000,000/gram, streptococcus fecalis original powder concentration is 40,000,000,000/gram, saccharomyces cerevisiae is former
Powder concentration be 20,000,000,000/gram, bacillus coagulans original powder concentration be 20,000,000,000/gram, clostridium butyricum original powder concentration be 20,000,000,000/gram.
(3) it ferments
Mixed-culture medium in step (2) is poured into fermentor, 28-32 DEG C be sealed by fermentation 4 days to get EM bacterium solution at
Product.
2, application method
(1) dosage of EM bacterium solution is calculated in per acre/rice water surface 2-3Kg ratio, is diluted with 50-100 times of clear water, directly
It uniformly splashes in water body, water pollutant improvement water quality of degrading can be played the role of at 15 DEG C of water temperature or more.
(2) EM bacterium solution is directly mixed with aquatic feeds in the ratio of 1-3wt%, is then fed.
Embodiment 2:
1, the preparation of EM bacterium solution
(1) fluid nutrient medium is prepared
By concentration calculation: molasses 17.5g/L, ammonium sulfate 7.5g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, phosphoric acid
Hydrogen dipotassium 3g/L, remaining is water.After the completion of preparation, the 20min that sterilizes is placed at 115 DEG C, it is spare after cooling.
(2) it is inoculated with
It takes lactobacillus acidophilus FTR-1 bacterium powder 2g, lactobacillus plantarum FTR-2 bacterium powder 3g, streptococcus fecalis FTR-3 bacterium powder 2g, make
Brewer yeast FTJ-1 bacterium powder 2g, bacillus coagulans FTY-10 bacterium powder 0.75g, clostridium butyricum FTD-1 bacterium powder 1g mixing, are made EM
Strain is spare；Strain is inoculated into the culture medium in 4.30Kg step (1).Each strain concentration of bacterium powder is the same as embodiment 1.
(3) it ferments
Mixed-culture medium in step (2) is poured into fermentor, 28-32 DEG C be sealed by fermentation 4 days to get EM bacterium solution at
Product.
2, application method
(1) dosage of EM bacterium solution is calculated in per acre/rice water surface 2-3Kg ratio, is diluted with 50-100 times of clear water, directly
It uniformly splashes in water body, water pollutant improvement water quality of degrading can be played the role of at 15 DEG C of water temperature or more.
(2) EM bacterium solution is directly mixed with aquatic feeds in the ratio of 1-3wt%, is then fed.
Embodiment 3:
1, the preparation of EM bacterium solution
(1) fluid nutrient medium is prepared
By concentration calculation: molasses 20g/L, ammonium sulfate 10g/L, magnesium sulfate 1.5g/L, potassium dihydrogen phosphate 3g/L, phosphoric acid hydrogen two
Potassium 2g/L, remaining is water.After the completion of preparation, the 20min that sterilizes is placed at 115 DEG C, it is spare after cooling.
(2) it is inoculated with
It takes lactobacillus acidophilus FTR-1 bacterium powder 3g, lactobacillus plantarum FTR-2 bacterium powder 2g, streptococcus fecalis FTR-3 bacterium powder 4g, make
Brewer yeast FTJ-1 bacterium powder 1g, bacillus coagulans FTY-10 bacterium powder 1g, clostridium butyricum FTD-1 bacterium powder 0.5g mixing, are made EM bacterium
Kind is spare；Strain is inoculated into the culture medium in 2.3Kg step (1).Each strain concentration of bacterium powder is the same as embodiment 1.
(3) it ferments
Mixed-culture medium in step (2) is poured into fermentor, 28-32 DEG C be sealed by fermentation 4 days to get EM bacterium solution at
Product.
2, application method
(1) dosage of EM bacterium solution is calculated in per acre/rice water surface 2-3Kg ratio, is diluted with 50-100 times of clear water, directly
It uniformly splashes in water body, water pollutant improvement water quality of degrading can be played the role of at 15 DEG C of water temperature or more.
(2) EM bacterium solution is directly mixed with aquatic feeds in the ratio of 1-3wt%, is then fed.
Embodiment 4:
EM bacterium solution obtained by embodiment 1-3 is applied to Hubei Hanchuan river crab cultivation pond, by 3 kgs/acre of rice water surface ratios
Bacterium solution is watered 100 times of dilutions and directly splashed in cultivating pool by example.Six ponds are chosen, are arranged control group 3, experimental group 3.
Ammonia nitrogen, nitrite and visibility change with time in detection water body.The results are shown in Table 1.
1 breeding water body water quality detection result of table (ammonia nitrogen, NO2- unit are mg/L, and transparency unit is cm)
As it can be seen from table 1 the experimental group in embodiment has apparent degradation to ammonia nitrogen and nitrite, water quality is saturating
Lightness be improved significantly, very low level can be dropped in third day ammonia nitrogen and The sub-salt.Each component in embodiment in EM bacterium can
To decompose the polluters such as the organic matter in water body, ammonia nitrogen rapidly, algae is converted by polluter and is easy the nutrients absorbed
Matter can promote the growth of algae, and water body algae lover, water quality is tender to feel well.Water body algae lover, algae can secrete vitamin, ammonia again
The substances such as base acid promote the more preferable growth of mushroom, reach bacterium phase algae and balance each other.The ammonia nitrogen and nitrite of control group be not obvious
It reduces, transparency is not also obviously improved.Due to some function bacteriums of shortage in control group, cause pollutant that cannot decompose in time,
Water body algae phase bacterium is mutually bad, water turbidity, and transparency is high.So the EM bacterium of aquaculture is used in embodiment obtained in 1-3
Liquid can be used to improve culture environment of aquatic products, improve culture efficiency.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of EM bacterium solution for aquaculture, which is characterized in that include following opportunistic pathogen kind: lactobacillus acidophilus FTR-1, plant
Lactobacillus FTR-2, streptococcus fecalis FTR-3, saccharomyces cerevisiae FTJ-1, bacillus coagulans FTY-10, clostridium butyricum FTD-1；The EM
Bacterium solution is prepared as follows to obtain: according to parts by weight, taking FTR-1,1-3 parts of plant cream bars of 1-3 parts of lactobacillus acidophilus
FTR-2,2-4 parts of bacterium FTR-3,1-3 parts of streptococcus fecalis saccharomyces cerevisiae FTJ-1,0.5-1 parts of bacillus coagulans FTY-10,0.5-1
Part clostridium butyricum FTD-1 mixing, it is spare to be made EM strain；Gained EM strain 0.1-0.5% by weight percentage is inoculated into training
It supports in base, inoculation mixed liquor is poured into fermentation cylinder for fermentation, EM bacterium solution finished product is made.
2. a kind of EM bacterium solution for aquaculture according to claim 1, which is characterized in that the culture medium be comprising
15-20g/L molasses, 5-10g/L ammonium sulfate, 0.5-1.5g/L magnesium sulfate, 1-3g/L potassium dihydrogen phosphate, 1-3g/L dipotassium hydrogen phosphate
Aqueous solution.
3. a kind of EM bacterium solution for aquaculture according to claim 2, which is characterized in that the culture medium is being prepared
After the completion, the 20min that sterilizes is placed at 115 DEG C, it is spare after cooling.
4. a kind of EM bacterium solution for aquaculture according to claim 1, which is characterized in that each opportunistic pathogen kind concentration are as follows:
Lactobacillus acidophilus 100,000,000,000/gram, lactobacillus plantarum 100,000,000,000/gram, streptococcus fecalis 40,000,000,000/gram, saccharomyces cerevisiae 20,000,000,000/gram, it coagulates
Knot bacillus 20,000,000,000/gram, clostridium butyricum 20,000,000,000/gram.
5. a kind of EM bacterium solution for aquaculture according to claim 1, which is characterized in that the fermentation condition is
28-32 DEG C sealing and fermenting 4-6 days.
6. any EM bacterium solution for aquaculture of claim 1-5 is adjusting the application in aquaculture system.
7. the application method of any EM bacterium solution for aquaculture of claim 1-5, which is characterized in that specific method
Are as follows: the dosage of EM bacterium solution is calculated in per acre/rice water surface 2-3Kg ratio, is diluted with 50-100 times of volume clear water, Direct Uniform
It splashes in water body, water temperature is at 15 DEG C or more.
8. the application method of any EM bacterium solution for aquaculture of claim 1-5, which is characterized in that specific method
Are as follows: EM bacterium solution is directly mixed with aquatic feeds in the ratio of 1-3wt%, is then fed.
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Cited By (4)
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| CN111316941A (en) * | 2020-03-03 | 2020-06-23 | 中国水产科学研究院珠江水产研究所 | Water-regulating disease-preventing bio-base for breeding freshwater breeding animal seedlings |
| CN111793575A (en) * | 2020-06-03 | 2020-10-20 | 惠州学院 | Complex microbial inoculant and application thereof in aquaculture |
| CN113402034A (en) * | 2021-05-27 | 2021-09-17 | 福州一诺维生物科技有限公司 | Intestinal flora regulator for aquaculture and preparation method thereof |
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